Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| a comparative survey of the prevalence of human parasites found in fresh vegetables sold in supermarkets and open-aired markets in accra, ghana. | consuming raw vegetables offers essential nutrients that one may not get when such vegetables are usually cooked. however, eating them raw may pose a great risk for transmissions of pathogens. such risks may be influenced by the sources of the vegetables and washing techniques used. the aim of the study was to compare the prevalence and diversity of parasitic pathogens associated with vegetables sold at the two types of markets in ghana and compare effectiveness of various washing techniques. | 2014 | 25424391 |
| efficacy of wash solutions in recovering cyclospora cayetanensis, cryptosporidium parvum, and toxoplasma gondii from basil. | parasitic diseases can be acquired by ingestion of contaminated raw or minimally processed fresh produce (herbs and fruits). the sensitivity of methods used to detect parasites on fresh produce depends in part on the efficacy of wash solutions in removing them from suspect samples. in this study, six wash solutions (sterile e-pure water, 3% levulinic acid-3% sodium dodecyl sulfate, 1 m glycine, 0.1 m phosphate-buffered saline, 0.1% alconox, and 1% hcl-pepsin) were evaluated for their effectivene ... | 2014 | 25198596 |
| [prevalence of intestinal microsporidia and other intestinal parasites in hiv positive patients from maracaibo, venezuela]. | to detect the presence of microsporidia and other enteric parasites in patients with hivaids of the autonomous services university hospital of maracaibo (sahum), where there are no previous studies in this field. | 2014 | 24652209 |
| burden of major diarrheagenic protozoan parasitic co-infection among amoebic dysentery cases from north east india: a case report. | intestinal diarrheagenic polyparasitic infections are among the major public health concerns in developing countries. here we examined stool specimens by microscopy, dna dot blot and polymerase chain reaction (pcr) to evaluate the co-infection of four principal protozoans among amoebic dysentery cases from northeast indian population. the multiplex pcr confirmed entamoeba histolytica (8.1%), entamoeba dispar (4.8%) and mixed infection of both the parasites (3.4%) in 68 of 356 stool specimens tha ... | 2015 | 26099490 |
| multicenter evaluation of the biofire filmarray gastrointestinal panel for etiologic diagnosis of infectious gastroenteritis. | the appropriate treatment and control of infectious gastroenteritis depend on the ability to rapidly detect the wide range of etiologic agents associated with the disease. clinical laboratories currently utilize an array of different methodologies to test for bacterial, parasitic, and viral causes of gastroenteritis, a strategy that suffers from poor sensitivity, potentially long turnaround times, and complicated ordering practices and workflows. additionally, there are limited or no testing met ... | 2015 | 25588652 |
| expert elicitation as a means to attribute 28 enteric pathogens to foodborne, waterborne, animal contact, and person-to-person transmission routes in canada. | enteric illness contributes to a significant burden of illness in canada and globally. understanding its sources is a critical step in identifying and preventing health risks. expert elicitation is a powerful tool, used previously, to obtain information about enteric illness source attribution where information is difficult or expensive to obtain. thirty-one experts estimated transmission of 28 pathogens via major transmission routes (foodborne, waterborne, animal contact, person-to-person, and ... | 2015 | 25835810 |
| cyclospora cayetanensis infection in transplant traveller: a case report of outbreak. | cyclospora cayetanensis is a protozoan parasite causing intestinal infections. a prolonged course of infection is often observed in immunocompromised individuals. in europe, less than 100 cases of c. cayetanensis infection have been reported to date, almost all of which being diagnosed in individuals after travelling abroad. | 2015 | 26249024 |
| zoonotic enteric parasites transmitted from dogs in egypt with special concern to toxocara canis infection. | this work aimed to study the role played by dogs in transmitting zoonotic enteric parasites to humans in egypt and to analyze the risk factors associated with the occurrence of such infection in dogs. serodiagnosis of anti-toxocara immunoglobulin g (igg) antibodies among human beings as well as analyzing risk factors predispose to toxocara canis infection in human beings are another objectives of this study. | 2015 | 27047182 |
| draft genome sequences from cyclospora cayetanensis oocysts purified from a human stool sample. | the parasite cyclospora cayetanensis causes foodborne diarrheal illness. here, we report draft genome sequences obtained from c. cayetanensis oocysts purified from a human stool sample. the genome assembly consists of 865 contigs with a total length of 44,563,857 bases. these sequences can facilitate the development of subtyping tools to aid outbreak investigations. | 2015 | 26586880 |
| cyclospora infection among school children in kathmandu, nepal: prevalence and associated risk factors. | the intestinal coccidian protozoa cyclospora cayetanensis has emerged as an important cause of parasitic diarrhea among children living in developing countries. this study aimed to determine the prevalence of cyclospora among the school children of kathmandu with reference to various associated risk factors. | 2015 | 26865822 |
| identification and morphologic and molecular characterization of cyclospora macacae n. sp. from rhesus monkeys in china. | cyclospora spp. in nonhuman primates are most closely related to cyclospora cayetanensis, an emerging human pathogen causing outbreaks of cyclosporiasis in north america. studies thus far indicate the possible existence of host specificity in cyclospora spp. in this study, 411 fecal specimens from free-range rhesus monkeys (macaca mulatta) were collected and examined for cyclospora by sequence analysis of the small subunit rrna gene. a novel cyclospora species was identified in 28 (6.8%) specime ... | 2015 | 25673080 |
| 2013 multistate outbreaks of cyclospora cayetanensis infections associated with fresh produce: focus on the texas investigations. | the 2013 multistate outbreaks contributed to the largest annual number of reported us cases of cyclosporiasis since 1997. in this paper we focus on investigations in texas. we defined an outbreak-associated case as laboratory-confirmed cyclosporiasis in a person with illness onset between 1 june and 31 august 2013, with no history of international travel in the previous 14 days. epidemiological, environmental, and traceback investigations were conducted. of the 631 cases reported in the multista ... | 2015 | 25865140 |
| a linear mitochondrial genome of cyclospora cayetanensis (eimeriidae, eucoccidiorida, coccidiasina, apicomplexa) suggests the ancestral start position within mitochondrial genomes of eimeriid coccidia. | the near complete mitochondrial genome for cyclospora cayetanensis is 6184 bp in length with three protein-coding genes (cox1, cox3, cytb) and numerous lsrdna and ssrdna fragments. gene arrangements were conserved with other coccidia in the eimeriidae, but the c. cayetanensis mitochondrial genome is not circular-mapping. terminal transferase tailing and nested pcr completed the 5'-terminus of the genome starting with a 21 bp a/t-only region that forms a potential stem-loop. regions homologous to ... | 2015 | 25812835 |
| cyclospora cayetanensis travels in tap water on italian trains. | tap water samples from the toilets of an italian national railway train were collected over a period of 10 months and tested for the presence of cyclospora cayetanensis (c. cayetanensis) using evagreen® real-time polymerase chain reaction (rt-pcr) assay coupled with high resolution melting (hrm) analysis for protozoan detection and oocyst quantification. c. cayetanensis positive samples were detected in march, april, and may 2013, with the number of oocysts of 4, 5, and 11 per liter, respectivel ... | 2015 | 25719480 |
| cyclospora infection linked to travel to mexico, june to september 2015. | cyclospora cayetanensis was identified in 176 returned travellers from the riviera maya region of mexico between 1 june and 22 september 2015; 79 in the united kingdom (uk) and 97 in canada. uk cases completed a food exposure questionnaire. this increase in reported cyclospora cases highlights risks of gastrointestinal infections through travelling, limitations in cyclospora surveillance and the need for improved hygiene in the production of food consumed in holiday resorts. | 2015 | 26536814 |
| prevalence of intestinal protozoa among saudi patients with chronic renal failure: a case-control study. | it has been hypothesized that chronic renal failure (crf) predisposes patients to infection with intestinal protozoa. we tested this hypothesis with a matched case-control study to determine the prevalence of these protozoa and their diarrhea associated symptoms among 50 patients with crf (cases) from taif, western saudi arabia. fifty diarrheal patients without crf were recruited in the study as controls. participants were interviewed by a structured questionnaire and stool samples were collecte ... | 2015 | 26491455 |
| epidemiology of cyclospora species in humans in malatya province in turkey. | cyclospora species are rare among other coccidia parasites and can cause recurrent gastroenteritis. cyclospora spp. can infect reptiles, insects, rodents, and mammals. | 2015 | 26421126 |
| detection of emergence cyclospora cayetanensis in a hiv(+) / aids patient with diarrhea from tehran: a case report. | coccidian protozoa of cyclospora cayetanensis are obligate intracellular apicomplexan parasites that infect the mucosal epithelium of the small intestine of immunocompetent and immunocompromised persons. a 25- years old woman from around, tehran with complaint of faintness and fatigue with hiv positive/aids confirmed eight months ago was admitted in imam khomeini hospital, tehran, iran in 2014. the patient suffered from intestinal and lung symptoms like watery diarrhea, flu-like symptoms. the st ... | 2015 | 26258100 |
| molecular detection of cyclospora in water, soil, vegetables and humans in southern italy signals a need for improved monitoring by health authorities. | to date, in europe, there is scant information on the occurrence of cyclospora in water from treatment plants and in humans, and no data are available on soil or fresh plant products. here, we undertook the first molecular survey of cyclospora in multiple biological matrices collected from the apulia region of southern italy. samples of irrigation water from four municipal treatment plants, eight different types of vegetables or fruit (cucumber, lettuce, fennel, celery, tomato, melon, endive and ... | 2015 | 26188495 |
| genetic similarities between cyclospora cayetanensis and cecum-infecting avian eimeria spp. in apicoplast and mitochondrial genomes. | cyclospora cayetanensis is an important cause for diarrhea in children in developing countries and foodborne outbreaks of cyclosporiasis in industrialized nations. to improve understanding of the basic biology of cyclospora spp. and development of molecular diagnostic tools and therapeutics, we sequenced the complete apicoplast and mitochondrial genomes of c. cayetanensis. | 2015 | 26152563 |
| molecular characterization of clinical isolates of cyclospora cayetanensis from patients with diarrhea in india. | cyclospora cayetanensis is an intestinal coccidian protozoan that has emerged as an important cause of both epidemic and endemic protracted diarrhea worldwide. though humans appear to be the only natural hosts; the role of animals as natural reservoir is uncertain but of increasing concern. the present study aimed to study the prevalence of coccidian in different groups such as immunocompromised, clinically apparent immunocompetent and healthy individuals. also, the study isolates were assessed ... | 2015 | 26068334 |
| the complete mitochondrial genome of the foodborne parasitic pathogen cyclospora cayetanensis. | cyclospora cayetanensis is a human-specific coccidian parasite responsible for several food and water-related outbreaks around the world, including the most recent ones involving over 900 persons in 2013 and 2014 outbreaks in the usa. multicopy organellar dna such as mitochondrion genomes have been particularly informative for detection and genetic traceback analysis in other parasites. we sequenced the c. cayetanensis genomic dna obtained from stool samples from patients infected with cyclospor ... | 2015 | 26042787 |
| infections by intestinal coccidia and giardia duodenalis. | the coccidians cryptosporidium spp, cyclospora cayetanensis, and cystoisospora belli and the flagellate giardia duodenalis are pathogenic protozoa associated with gastrointestinal manifestations. diagnosis relies heavily on microscopy, and although ova-and-parasite examinations can detect giardia and cystoisospora, cryptosporidium and cyclospora often require specific diagnostic requests. approved non-microscopy methods are available for giardia and cryptosporidium, although negative results are ... | 2015 | 26004650 |
| detection of cyclospora in captive chimpanzees and macaques by a quantitative pcr-based mutation scanning approach. | cyclospora is a protistan parasite that causes enteritis in several species of animals including humans. the aim of this study was to investigate the presence of cyclospora in captive non-human primates. | 2015 | 25972100 |
| multilocus sequence typing tool for cyclospora cayetanensis. | because the lack of typing tools for cyclospora cayetanensis has hampered outbreak investigations, we sequenced its genome and developed a genotyping tool. we observed 2 to 10 geographically segregated sequence types at each of 5 selected loci. this new tool could be useful for case linkage and infection/contamination source tracking. | 2016 | 27433881 |
| prevalence of enteric protozoan oocysts with special reference to sarcocystis cruzi among fecal samples of diarrheic immunodeficient patients in iran. | the genus sarcocystis is not usually considered as an important enteric pathogen in immune compromised patients. it might be expected that species for which humans are the final host (sarcocystis hominis and sarcocystis suihominis as well as possibly others) would be encountered increasingly often in immunodeficient persons. this study aimed to address how to detect and differentiate sarcocystis oocysts and/or sporocysts from enteric protozoans in the diarrheal samples of immunodeficient patient ... | 2016 | 27417091 |
| evaluation of library preparation methods for illumina next generation sequencing of small amounts of dna from foodborne parasites. | illumina library preparation methods for ultra-low input amounts were compared using genomic dna from two foodborne parasites (angiostrongylus cantonensis and cyclospora cayetanensis) as examples. the ovation ultralow method resulted in libraries with the highest concentration and produced quality sequencing data, even when the input dna was in the picogram range. | 2016 | 27553132 |
| marked seasonality of cyclospora cayetanensis infections: ten-year observation of hospital cases, honduras. | document seasonality occurrence and epidemiologic characteristics of cyclospora cayetanensis infections during a 10-year period from patients consulting at the university hospital, honduras. | 2016 | 26847438 |
| wide genetic variations at 18s ribosomal rna locus of cyclospora cayetanensis isolated from egyptian patients using high resolution melting curve. | a variable clinical picture of cyclosporiasis including gastrointestinal tract (git) symptomatic or asymptomatic beside extraintestinal consequences suggests a possibility of heterogenicity of cyclospora cayetanensis. the present work aimed to explore the possibility of genetic variation of c. cayetanensis using high-resolution melting (hrm) curve of polymerase chain reaction (pcr) amplified 18s rrna genes. dnas extracted from the stool samples of 70 cyclosporiasis patients were amplified and sc ... | 2016 | 27041342 |
| cyclospora cayetanensis, opportunistic protozoan parasite, in van province, turkey: a report of seven cases. | the aim of this study is to report seven cyclospora cayetanensis (c.cayetanensis) cases determined in yuzuncu yil university medical faculty parasitology laboratory, van province, turkey. in the study native-lugol, formalin-ethyl acetate and modified acid-fast staining methods were performed to stool samples of the patients sent from outpatient clinics of the hospital. all of the patients infected with c. cayetanensis were older than 15 years. in our cases there were not a history of internation ... | 2016 | 27905288 |
| replication and maintenance of the plasmodium falciparum apicoplast genome. | members of the phylum apicomplexa are responsible for many devastating diseases including malaria (plasmodium spp.), toxoplasmosis (toxoplasma gondii), babesiosis (babesia bovis), and cyclosporiasis (cyclospora cayetanensis). most apicomplexans contain a unique and essential organelle called the apicoplast. derived from an ancient chloroplast, the apicoplast replicates and maintains a 35 kilobase (kb) circular genome. due to its essential nature within the parasite, drugs targeted to proteins in ... | 2016 | 27338018 |
| comparative genomics reveals cyclospora cayetanensis possesses coccidia-like metabolism and invasion components but unique surface antigens. | cyclospora cayetanensis is an apicomplexan that causes diarrhea in humans. the investigation of foodborne outbreaks of cyclosporiasis has been hampered by a lack of genetic data and poor understanding of pathogen biology. in this study we sequenced the genome of c. cayetanensis and inferred its metabolism and invasion components based on comparative genomic analysis. | 2016 | 27129308 |
| comparative sequence analysis of cyclospora cayetanensis apicoplast genomes originating from diverse geographical regions. | cyclospora cayetanensis is an emerging coccidian parasite that causes endemic and epidemic diarrheal disease called cyclosporiasis, and this infection is associated with consumption of contaminated produce or water in developed and developing regions. food-borne outbreaks of cyclosporiasis have occurred almost every year in the usa since the 1990s. investigations of these outbreaks are currently hampered due to lack of molecular epidemiological tools for trace back analysis. the apicoplast of c. ... | 2016 | 27899155 |
| molecular appraisal of intestinal parasitic infection in transplant recipients. | diarrhoea is the main clinical manifestation caused by intestinal parasitic infections in patients, with special reference to transplant recipients who require careful consideration to reduce morbidity and mortality. further, molecular characterization of some important parasites is necessary to delineate the different modes of transmission to consider appropriate management strategies. we undertook this study to investigate the intestinal parasitic infections in transplant recipients with or wi ... | 2016 | 27934806 |
| neglected waterborne parasitic protozoa and their detection in water. | outbreak incidents raise the question of whether the less frequent aetiological agents of outbreaks are really less frequent in water. alternatively, waterborne transmission could be relevant, but the lack of attention and rapid, sensitive methods to recover and detect the exogenous stages in water may keep them under-recognized. high quality information on the prevalence and detection of less frequent waterborne protozoa, such as cyclospora cayetanensis, toxoplasma gondii, isospora belli, balan ... | 2016 | 27281375 |
| intestinal parasitic infections in relation to cd4(+) t cell counts and diarrhea in hiv/aids patients with or without antiretroviral therapy in cameroon. | intestinal parasitic infections (ipi) are a major public health concern in hiv/aids patients particularly in resource-limited settings of sub-saharan africa. studies investigating the relationship between intestinal parasitic infections and cd4(+) t cell counts and diarrhea in hiv/aids patients with or without antiretroviral therapy in the region are not readily available hence the need to perform this study. | 2016 | 26754404 |
| multiplex-touchdown pcr to simultaneously detect cryptosporidium parvum, giardia lamblia, and cyclospora cayetanensis, the major causes of traveler's diarrhea. | this study aimed to develop a multiplex-touchdown pcr method to simultaneously detect 3 species of protozoan parasites, i.e., cryptosporidium parvum, giardia lamblia, and cyclospora cayetanensis, the major causes of traveler's diarrhea and are resistant to standard antimicrobial treatments. the target genes included the cryptosporidium oocyst wall protein for c. parvum, glutamate dehydrogenase for g. lamblia, and 18s ribosomal rna (18s rrna) for c. cayetanensis. the sizes of the amplified fragme ... | 2016 | 27853120 |
| detection and prevalence of protozoan parasites in ready-to-eat packaged salads on sale in italy. | to investigate the prevalence of protozoan contamination by giardia duodenalis, cryptosporidium spp., toxoplasma gondii and cyclospora cayetanensis, in 'ready to eat' (rte) salads on sale in italy, 648 packages were purchased from industrial and local brands. nine individual packages from each brand were collected per month, pooled and subjected to microscopy and molecular analyses. microscopic examination of 864 slides detected cryptosporidium spp. but also blastocystis hominis and dientamoeba ... | 2017 | 28648295 |
| simultaneous molecular detection of cryptosporidium and cyclospora from raw vegetables in korea. | cryptosporidium and cyclospora are well-known coccidian protozoa that can cause waterborne and foodborne diarrheal illnesses. there have been a few reports regarding contamination in different vegetables with cryptosporidium, but no data are available regarding the sources of cyclospora infections in korea. in the present study, we collected 6 kinds of vegetables (perilla leaves, winter-grown cabbages, chives, sprouts, blueberries, and cherry tomatoes) from july 2014 to june 2015, and investigat ... | 2017 | 28506035 |
| [intestinal coccidiosis in children admitted to a hospital in peru and comparison of two methods for detecting cryptosporidium spp]. | a cross-sectional study was done between october 2014 and june 2015 to estimate the frequency of infection due to intestinal coccidiosis in children admitted to a hospital in peru, and compare the modified acid-fast staining procedure with the enzyme-linked immunoassay (elisa) method for the detection of cryptosporidium spp. coccidia were detected using the modified acid-fast staining procedure and the cryptosporidium elisa method (kit r-biopharm) on seriated stool samples. out of a total of 325 ... | 2017 | 28327845 |
| first report of tunisian coastal water contamination by protozoan parasites using mollusk bivalves as biological indicators. | in order to establish seawater contamination by emerging protozoan parasites, we used qpcr to molecularly characterize and evaluate the parasitic burden of giardia duodenalis, cryptosporidium spp., toxoplasma gondii, and cyclospora cayetanensis in 1255 wild bivalve mollusks collected along the tunisian coasts. t. gondii, g. duodenalis and c. cayetanensis were detected in 6.9% (99% ci=1.6-12.2%) pools of ruditapes decussatus. none of the samples were found positive to cryptosporidium spp.; 6.6% p ... | 2017 | 28168979 |
| evaluation of an improved u.s. food and drug administration method for the detection of cyclospora cayetanensis in produce using real-time pcr. | cyclospora cayetanensis is a protozoan parasite that causes human diarrheal disease associated with the consumption of fresh produce or water contaminated with c. cayetanensis oocysts. in the united states, foodborne outbreaks of cyclosporiasis have been linked to various types of imported fresh produce, including cilantro and raspberries. an improved method was developed for identification of c. cayetanensis in produce at the u.s. food and drug administration. the method relies on a 0.1% alcono ... | 2017 | 28590822 |
| [cyclospora cayetanensis]. | 2017 | 28394980 | |
| multilocus sequence typing and clonal population genetic structure of cyclospora cayetanensis in humans. | to investigate the prevalence of cyclospora cayetanensis in a longitudinal study and to conduct a population genetic analysis, fecal specimens from 6579 patients were collected during the cyclosporiasis - prevalent seasons in two urban areas of central china in 2011-2015. the overall incidence of c. cayetanensis infection was 1·2% (76/6579): 1·6% (50/3173) in zhengzhou and 0·8% (26/3406) in kaifeng (p 0·05). all the isolates clustered in the c. cayetanensis clade based on the small subunit ribos ... | 2017 | 28697813 |
| the prevalence of cyclospora cayetanensis and cryptosporidium spp. in turkish patients infected with hiv-1. | opportunistic infections such as cryptosporidiosis and cyclosporiasis are commonly encountered in patients with acquired immunodeficiency syndrome (aids). we investigated the existence of opportunistic protozoans that significantly affect the quality of life in hiv-1 infected patients using conventional and molecular methods. the study group comprised 115 hiv-1 positive patients. in the identification of cyclospora cayetanensis and cryptosporidium, the formol-ether precipitation method was used ... | 2017 | 28682770 |