Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| prediction of fungal growth and ochratoxin a production by aspergillus ochraceus on irradiated barley grain as influenced by temperature and water activity. | ochratoxin a (ota) is a secondary metabolite of aspergillus and penicillium species, including aspergillus ochraceus, a species that can be found in stored cereal grains such as barley. the objective of this study was to determine the effects of water activity (a(w), 0.80-0.99), temperature (10, 20, 30 degrees c), and a. ochraceus isolate differences on radial growth and ota production in irradiated barley grains. the three isolates showed optimal conditions for growth and ochratoxin a productio ... | 2004 | 15240077 |
| genetic relationships among brazilian strains of aspergillus ochraceus based on rapd and its sequences. | ochratoxin a (oa) is a mycotoxin that has been found in coffee beans and coffee beverages. its toxicological profile includes carcinogenicity, nephrotoxicity, and immunotoxicity. aspergillus ochraceus is the major species responsible for oa production in brazilian coffee beans. the genetic relationships among 25 a. ochraceus strains collected from brazilian coffee-bean samples were determined based on rapd and internal transcribed spacer (its) sequence data. the isolates were resolved into 2 dis ... | 2004 | 15644917 |
| cloning and expression of clt genes encoding milk-clotting proteases from myxococcus xanthus 422. | the screening of a gene library of the milk-clotting strain myxococcus xanthus 422 constructed in escherichia coli allowed the description of eight positive clones containing 26 open reading frames. only three of them (clta, cltb, and cltc) encoded proteins that exhibited intracellular milk-clotting ability in e. coli, saccharomyces cerevisiae, and pichia pastoris expression systems. | 2004 | 15466588 |
| chemical composition and in vitro antimicrobial activities of the essential oils from endemic psiadia species growing in mauritius. | the composition of the leaf oils, obtained by hydrodistillation, of five endemic psiadia species of the asteraceae family were studied by gc/ms on both polar and non-polar columns. the analysis showed that the volatile components of the oils were made up essentially of monoterpenes, sesquiterpenes, aliphatics and other shikimic acid derivatives. with respect to the non-volatile components, great variations were observed: p. lithospermifolia contained (e)-isoasarone (51.5%); p. penninervia: eugen ... | 2004 | 15516729 |
| modelling of effects of water activity and temperature on germination and growth of ochratoxigenic isolates of aspergillus ochraceus on a green coffee-based medium. | influence of water activity (0.75-0.99 a(w)) and temperature (10, 20 and 30 degrees c) on germination and mycelial growth on green coffee extract agar medium of three ochratoxigenic isolates of aspergillus ochraceus was studied. optimal conditions for germination and growth were observed at 0.95-0.99 a(w) and 20-30 degrees c for the three isolates. minimum a(w) level for germination was 0.80, and 0.85 for mycelial growth. at marginal a(w) and temperature levels assayed, the lag phases prior to g ... | 2005 | 15617796 |
| ochratoxin a-induced dna damage in human fibroblast: protective effect of cyanidin 3-o-beta-d-glucoside. | ochratoxin a (ota), a mycotoxin produced by aspergillus ochraceus and other moulds, has recently received growing attention because of its carcinogenic, teratogenic and nephrotoxic properties in both humans and farm animals. nevertheless, with regard to the mechanism of toxicity, the data in the literature are inconclusive. the aim of our work was to verify in human fibroblasts treated with different ota dosages the involvement of oxidative pathway in the damage mechanism of this mycotoxin and t ... | 2005 | 15629238 |
| extraction of milk-clotting enzyme produced by solid state fermentation of aspergillus oryzae. | studies on the extraction of milk-clotting enzyme after solid-state fermentation (ssf) of wheat bran by a local strain of aspergillus oryzae ls1 were done. the extraction of the enzyme was found to be depended on different parameters like nature of extractant, soaking time, temperature etc. from different inorganic and organic extractants, calcium chloride (0.05%) and glycerol (40%) were found to be the best solvents for leaching out milk-clotting enzyme. the optimum volume of calcium chloride w ... | 2005 | 16450841 |
| incidence of toxigenic fungi and ochratoxin a in dried fruits sold in brazil. | a total of 117 dried fruit samples (black sultanas, white sultanas, dates, dried plums, dried figs and apricots) from different origins were analysed both for toxigenic fungi and for the presence of ochratoxin a. amongst the fungi found, aspergillus niger was predominant, with 406 isolates, of which 15% were ochratoxin a producers. they were followed by a. ochraceus, with 15 isolates and 87% ochratoxigenics, and a. carbonarius, with only five isolates of which 60% were ochratoxin a producers. th ... | 2005 | 16356890 |
| influence of volatile compounds produced by yeasts predominant during processing of coffea arabica in east africa on growth and ochratoxin a (ota) production by aspergillus ochraceus. | the effects of volatile compounds produced during coffee processing by pichia anomala, p. kluyveri and hanseniaspora uvarum on growth of aspergillus ochraceus and production of ochratoxin a (ota) were studied. on malt extract agar (mea) and on coffee agar (ca), exposure of a. ochraceus to the gaseous phase of malt yeast glucose peptone (mygp) plates inoculated with p. anomala, p. kluyveri and h. uvarum inhibited fungal growth, with the two pichia spp. showing the strongest effect. the main ester ... | 2005 | 16240461 |
| inhibitory activity of phosphates on molds isolated from foods and food processing plants. | six commercial phosphates were evaluated for inhibition of the growth of 17 molds isolated from food sources. the assays were performed at neutral and natural (without ph adjustment) ph values, and the molds were streaked on plate count agar with added phosphates. phosphate concentrations of 0.1, 0.3, 0.5, 1.0, and 1.5% (wt/vol) were used, and the mic was determined. the resistance of molds to phosphates depended on the species. at a neutral ph, aspergillus ochraceus and fusarium proliferatum we ... | 2005 | 16300093 |
| conditions of formation of ochratoxin a in drying, transport and in different commodities. | the major species of fungi responsible for ochratoxin production (ota) in a range of commodities are penicillium verrucosum, aspergillus ochraceus and aspergillus section nigri, especially a. carbonarius. p. verrucosum is particularly important in northern europe where damp cooler conditions occur and where drying regimes need to be efficient and effective for preventing post-harvest contamination with ota. a. ochraceus can infect cereals including barley, maize, coffee, cocoa and edible nuts. a ... | 2005 | 16332616 |
| circular dichroism analysis of destruxins from metarhizium anisopliae. | destruxins are secondary metabolites secreted by metarhizium anisopliae [y. kodaira, toxic substances to insects, produced by aspergillus ochraceus and oopsra destructor, agric. biol. chem., 25 (1961) 261-262. d.w. roberts, toxins from the entomogenous fungus metarhizium anisoplaie: isolation from submerged cultures, j. invertebr. pathol., 14 (1969) 82-88. d.w. roberts, toxins from the entomogenic fungi in microbial control of pest and plant disease, academic press, new york, 1981, pp441-464.]. ... | 2005 | 15656942 |
| effect of water activity and temperature on mycelial growth and ochratoxin a production by isolates of aspergillus ochraceus on irradiated green coffee beans. | aspergillus ochraceus as a fungal contaminant and ochratoxin a (ota) producer plays an important role in coffee quality. temperature and water activity (a(w)) significantly influence mycelial growth and ota production by isolates of a. ochraceus on green coffee beans. maximum mycelial growth was found at 30 degrees c and 0.95 to 0.99 a(w). a marked decrease in growth rate was observed when temperature and a(w) were reduced. at 0.80 a(w), mycelial growth occurred only at 30 and 20 degrees c for o ... | 2005 | 15690814 |
| volatile compounds of aspergillus strains with different abilities to produce ochratoxin a. | volatile compounds emitted by aspergillus strains having different abilities to produce ochratoxin a were investigated. thirteen strains of aspergillus ochraceus, three belonging to the a. ochraceus group, and eight other species of aspergillus were examined for their abilities to produce volatile compounds and ochratoxin a on a wheat grain medium. the profiles of volatile compounds, analyzed using spme, in all a. ochraceus strains, regardless of their toxeginicity, were similar and comprised ma ... | 2005 | 15740058 |
| determination of ochratoxin a in wine by liquid chromatography tandem mass spectrometry after combined anion-exchange/reversed-phase clean-up. | ochratoxin a (ota) is a mycotoxin produced by aspergillus ochraceus and penicillium verrucosum. it has been found and analysed in several foods and feeds. owing to its toxicity and occurrence in food and feed, the european community has issued directives and some countries have their own regulations for ota contents in food, feed and beverages. this work describes a method for the determination of ota in mulled and red wine. it is based on combined anion exchange/reversed-phase clean-up and was ... | 2005 | 15770471 |
| characterization and regulation of new secondary metabolites from aspergillus ochraceus m18 obtained by uv mutagenesis. | uv irradiation of aspergillus ochraceus nrrl 3174 conidia led to stable mutations in ochratoxin and penicillic-acid pathways. these mutants, especially m18, produced an unexpectedly large number of new metabolites. two new compounds were purified by tlc and hplc and their chemical structures were determined. they are 2,10-dimethyl 4-hydroxy-6-oxo-4-undecen-7-yne (1) and 4-(3-methyl-2- butenyl) oxy 1-phenyl acetic acid (2). compound 1 is very active against gram-positive bacteria, such as staphyl ... | 2005 | 15782235 |
| biotransformation and nephrotoxicity of ochratoxin b in rats. | ochratoxin b (otb), a secondary metabolite of aspergillus ochraceus, is the nonchlorinated analogue of the mycotoxin ochratoxin a (ota), which is one of the most potent renal carcinogens in rodents. despite the closely related structure, otb is considered to be of much lower toxicity. ota is poorly metabolized and slowly eliminated, and this may play an important role in ota toxicity, carcinogenicity, and organ specificity. since little is known regarding biotransformation and renal toxicity of ... | 2005 | 15963343 |
| pcr detection assays for the ochratoxin-producing aspergillus carbonarius and aspergillus ochraceus species. | two pcr assays have been developed to detect aspergillus carbonarius and aspergillus ochraceus, considered the main sources of ochratoxin a (ota) contaminating commodities, particularly grapes, coffee and derivatives, in warm climates. the species specific primers have been designed on the basis of its (internal transcribed spacers of rdna units) sequence comparisons obtained from aspergillus strains and have been tested in a number of strains from different origins and hosts. these pcr assays, ... | 2005 | 15967531 |
| two primer pairs to detect ota producers by pcr method. | fungi contaminating foods and feeds may produce many mycotoxins including ochratoxin a (ota). early and rapid detection of potential ota producing fungi is important to reduce the negative impacts of ota. in this study, two pcr specific primer pairs, aolc35-12l/aolc35-12r and aootal/aootar, were designed from a dna sequence of a polyketide synthase gene in aspergillus ochraceus nrrl 3174. on 14 different fungi tested by pcr, aolc35-12l/aolc35-12r amplified a unique band from either ota or citrin ... | 2005 | 15975678 |
| occurrence of mycotoxin producing fungi in bee pollen. | the natural mycobiota occurring in bee pollen is studied in the present report with special attention to analyze the incidence of fungal species that are potential producers of mycotoxins. a total of 90 ready-to-eat bee pollen samples were analyzed. eighty-seven samples were collected in stores placed in different spanish areas and three were from buenos aires (argentina). the statistical results (anova) showed that yeasts and penicillium spp. were the predominant fungi. with regard to the poten ... | 2005 | 16009441 |
| effect of the post-harvest processing procedure on ota occurrence in artificially contaminated coffee. | the purpose of this work was to study how the type of post-harvest process, i.e. natural preparation known as the dry method, and two wet processes, affected contamination and toxin production up to the green coffee stage. batches were contaminated with ochratoxin a or with ota-producing strains of aspergillus ochraceus and aspergillus niger. for ota artificial contamination, hulling or husk removal caused a reduction of ota. when a. ochraceus was inoculated at low level, its growth was hampered ... | 2005 | 16023238 |
| microfungi in cultivated fields in eskişehir provience (turkey). | the soil microfungi flora was investigated in four locations of eskişehir (turkey). 56 soil samples were seasonaly collected from 14 stations in the areas of karacahöyük, bahçecik, ogu i, and ogu ii. a total of 110 species belonging to 32 genera were encountered including absidia, acremonium, alternaria, aspergillus, beauveria, botryoderma, chaetomium, chrysosporium, cladosporium, eupenicillium, eurotium, fusarium, geotrichum, gliocladium, gonytrichum, metarrhizium, mucor, myrothecium, paecilomy ... | 2005 | 16028200 |
| stability of ochratoxin a (ota) during processing and decaffeination in commercial roasted coffee beans. | the fate of ochratoxin a (ota) during the processing of artificially contaminated green coffee beans, the effect of decaffeination on the production of ota in green and roasted coffee beans, and the effect of caffeine on the growth and ota production by aspergillus ochraceus were studied. the data indicated that the roasting, milling and decoction (brewing and turkish coffee making) processes caused different percentage reductions in ota. decaffeinated samples showed a significantly higher conce ... | 2005 | 16147432 |
| circumdatin h, a new inhibitor of mitochondrial nadh oxidase, from aspergillus ochraceus. | circumdatin h (1), a new alkaloid from the culture broth of aspergillus ochraceus, has been isolated, together with a known circumdatin, circumdatin e (2) and other known compounds: flavacol (3) and stephacidin a (4). the structure of 1 was established on the basis of chemical and spectral evidence. all of these alkaloids showed biological activity as inhibitors of the mammalian mitochondrial respiratory chain. | 2005 | 16156520 |
| a comparative study of extraction apparatus in hplc analysis of ochratoxin a in muscle. | ochratoxin a (ota) is a secondary fungal metabolite produced by several moulds, mainly by aspergillus ochraceus and by penicillium verrucosum, that occurs in meat products. the aim of this work was to optimize an efficient extraction procedure for the determination of ota in muscle tissue in order to assess its occurrence in meat samples. three different apparatus, a waring blender, a switching apparatus, and an ultrasonic processor, were evaluated to verify the efficiency of extraction. the ana ... | 2005 | 16158296 |
| conformational analysis of ochratoxin a by nmr spectroscopy and computational molecular modeling. | two-dimensional nmr spectroscopy has been used for a complete assignment of the proton and carbon-13 spectra of the metabolite from aspergillus ochraceus, ochratoxin a. in addition, phase-sensitive nuclear overhauser effect spectrometry experiments and computational molecular modeling (mm2 and mmff force field programs) have been employed to examine the conformational properties of ochratoxin a in chloroform solutions. particular attention has been given to intramolecular hydrogen-bonding format ... | 2005 | 16853154 |
| non-specificity of nutritional substrate for ochratoxin a production by isolates of aspergillus ochraceus. | aspergillus ochraceus is an important contaminant of diverse substrates, such as cereals, coffee, grapes and derivates. this fungus produce a nephrotoxic metabolite, ochratoxin a (ota), whose presence on food and feeds may be an important risk for animal and human health. the aim of this work was to evaluate the significance of the origin of a. ochraceus isolates on their ota production patterns on different substrates (yeast extract sucrose (yes) broth, irradiated barley grains, irradiated gree ... | 2006 | 16943024 |
| study of aflatoxin b1 and ochratoxin a production by natural microflora and aspergillus parasiticus in black and green olives of greek origin. | aflatoxin b1 (afb1) is a carcinogenic metabolite produced by certain aspergillus species. ochratoxin a (ota) is classified as "possible carcinogen" and it is a metabolite of aspergillus ochraceus and penicillium verrucosum. fungi contaminate natural and processed olives which support afb1 and ota production. the aim of this study was to compare and investigate afb1 and ota production in three different varieties of damaged olives. for each variety two different treatments were applied: (1) olive ... | 2006 | 16943059 |
| mycotoxin contamination of food in europe: early detection and prevention strategies. | this paper reviews the early detection and prevention strategies which have been employed in europe for the control of mycotoxin contamination of food in the context of a hazard analysis critical control point (haccp) framework. the critical control points (ccps) in the whole food chain where mycotoxins such as trichothecenes and ochratoxins are important have been identified. ecological studies on the effect of environmental factors which are marginal for growth and mycotoxin production have be ... | 2006 | 16944291 |
| diaporthin and orthosporin from the fruiting body of daldinia concentrica. | in our continuing study on the chemical constituents in the fruiting bodies of daldinia concentrica, diaporthin and orthosporin were isolated. their chemical structures were assigned based on various spectral studies. diaporthin and orthosporin, phytotoxins previously found in aspergillus ochraceus, were isolated from wood-rotting mushroom d. concentrica for the first time. | 2006 | 24039468 |
| bioconversion of straw into improved fodder: preliminary treatment of rice straw using mechanical, chemical and/or gamma irradiation. | crude protein (cp) content of mechanically ground rice straw into small particles by an electric grinder and reducing value (rv) and soluble protein (sp) in the culture filtrate were lower than that of the chopped straw into 5~6 cm lengths when both ground and chopped straws were fermented with aspergillus ochraceus, a. terreus or trichoderma koningii, at steady conditions. the reduction rate of rv, sp and cp was 22.2, 2.4, 7.3%; 9.1, 4.9, 8.5% or 0.0, 0.0, 3.6% for the three fungi, respectively ... | 2006 | 24039464 |
| the effects of yeasts involved in the fermentation of coffea arabica in east africa on growth and ochratoxin a (ota) production by aspergillus ochraceus. | the effects of pichia anomala, pichia kluyveri and hanseniaspora uvarum predominant during coffee processing on growth of aspergillus ochraceus and production of ochratoxin a (ota) on malt extract agar (mea) and on coffee agar (ca) were studied. the three yeasts were able to inhibit growth of a. ochraceus when co-cultured in mea and ca. growth inhibition was significantly higher on mea than on ca. furthermore, p. anomala and p. kluyveri were found to have a stronger effect on growth of a. ochrac ... | 2006 | 16213049 |
| chemical characterization and quantification of siderophores produced by marine and terrestrial aspergilli. | ten aspergilli (five each from marine and terrestrial habitats) were screened for siderophore production. all test isolates produced siderophores as indicated by a positive reaction in the fecl(3) test, chrome azurol sulphonate assay, and chrome azurol sulphonate agar plate test. further, the test isolates were compared for their siderophore production potential and chemical characteristics. examination of the chemical nature of the siderophores revealed that all test isolates produced hydroxama ... | 2006 | 16788730 |
| fungi and ochratoxin a detected in healthy grapes for wine production. | the mycoflora of healthy grapes (i.e. without visible symptoms of rot) for wine production in portuguese wine-making regions was assessed and its potential for ochratoxin a (ota) production evaluated. the ota content of grapes was also determined. | 2006 | 16411918 |
| an enzyme-linked immunosorbent assay for monitoring of aspergillus ochraceus growth in coffee powder, chilli powder and poultry feed. | the work was carried out to develop an immunoassay for estimation of aspergillus ochraceus biomass on solid substrate. | 2006 | 16411921 |
| influence of water activity, temperature and time on mycotoxins production on barley rootlets. | the objective of this study was to determine the ochratoxin (ot) and aflatoxin (af) production by three strains of aspergillus spp. under different water activities, temperature and incubation time on barley rootlets (br). | 2006 | 16441385 |
| molecular characterization of ochratoxin a biosynthesis and producing fungi. | 2006 | 16509448 | |
| ochratoxin a biosynthetic genes in aspergillus ochraceus are differentially regulated by ph and nutritional stimuli. | expression of the polyketide synthase (pks) gene which is involved in ochratoxin a (ota) biosynthesis in aspergillus ochraceus is linked to production of the mycotoxin, with high levels of pks mrna accumulation occurring in cultures producing ota, as assessed by real-time reverse transcription (rt)-pcr. ota production is regulated by nutrient availability, with supplementation of ota restrictive potato dextrose broth with yeast extract resulting in a 39-fold increase in production of the mycotox ... | 2006 | 16517184 |
| effect of dietary honey on intestinal microflora and toxicity of mycotoxins in mice. | bee honey is a functional food which has a unique composition, antimicrobial properties and bifidogenic effect. in order to assess whether honey can inhibit the toxic effect of mycotoxins, the present study was undertaken. | 2006 | 16533410 |
| ecophysiology of ochratoxigenic aspergillus ochraceus and penicillium verrucosum isolates. predictive models for fungal spoilage prevention - a review. | ochratoxin a (ota) is a secondary metabolite produced by several species of aspergillus and penicillium; among them aspergillus ochraceus and penicillium verrucosum are two ochratoxigenic species capable of growing in different climates and thus contamination of food crops with ota can occur worldwide. ota can be found in a wide range of foods such as cereals, coffee, cocoa, spices, beer, wine, dried vine fruit, grapes and meat products. ota is toxic to animals, it presents neurotoxic, immunotox ... | 2006 | 16546886 |
| ochratoxin a: its cancer risk and potential for exposure. | ochratoxin a (oa) is a naturally occurring mycotoxin known to contaminate a variety of foods and beverages. the cancer risk posed by oa was reviewed as relevant to human exposure, regulatory activities, and risk management efforts occurring worldwide, particularly in europe. oa moves through the food chain and has been found in the tissues and organs of animals, including human blood and breast milk. results from the national toxicology program's rodent bioassays show significantly increased inc ... | 2006 | 16621780 |
| amplification and diversity analysis of ketosynthase domains of putative polyketide synthase genes in aspergillus ochraceus and aspergillus carbonarius producers of ochratoxin a. | the diversity of polyketide synthase (pks) genes in aspergillus ochraceus nrrl 3174 and aspergillus carbonarius 2mu134 has been investigated using different primer pairs previously developed for the ketosynthase (ks) domain of fungal pkss. nine different ks domain sequences in a. ochraceus nrrl 3174 as well as five different ks domain sequences in a. carbonarius 2mu134 have been identified. the identified ks fragments were distributed in five different clusters on the phylogenetic tree, indicati ... | 2006 | 16715542 |
| occurrence of ochratoxin a in wine and ochratoxigenic mycoflora in grapes and dried vine fruits in south america. | grape and wine production in south america represents about 6.6% and 10% respectively of the world grape and wine production. the available information on the ochratoxigenic mycoflora and ochratoxin a (ota) presence in wine grapes, wines, grape juices and dried vine fruits is limited. surveys have been carried out in argentina and brazil which showed that aspergillus niger aggregate are predominant in the argentinean varieties while from the brazilian varieties the species a. niger, aspergillus ... | 2006 | 16716424 |
| intergeneric relationship between the aspergillus ochraceous virus f and the penicillium stoloniferum virus s. | it was reported that the "slow" component (psv-s) of penicillium stoloniferum virus complex also occurred in a second genus, aspergillus ochraceous. the responsible virus for this intergeneric occurrence was considered to be the "fast" component (aov-f) of a. ochraceous virus complex. in this investigation, aov dsrna 1, that was previously shown to cross-hybridize with psv-s dsrna, has been cloned. it was 1754 bp in length and contained one open reading frame of 539 amino acids (p63), had the sa ... | 2006 | 16725223 |
| [ochratoxin a contamination of cereal grain harvested in 2003 and 2004 years]. | the analysis of ochratoxin a--mycotoxin produced by widely distributed aspergillus and penicillium--of cereal grain harvested in 2003 - 2004 years were performed by immunoaffinity column clean-up and hplc with fluorescence detection. this survey examined 282 samples of raw grain wheat, rye, barley and oat 13.8% of all samples contaminated by ochratoxin a in the range 0.2-33.3 mg/kg. calculation made on the basis of the obtained means showed that the daily ochratoxin a intake of human from cereal ... | 2006 | 16739607 |
| antimicrobial activity of bacillus subtilis and bacillus pumilus during the fermentation of african locust bean (parkia biglobosa) for soumbala production. | to examine predominant isolates of bacillus subtilis and b. pumilus isolated from soumbala for their antimicrobial activity against indicator microorganisms as micrococcus luteus, staphyloccocus aureus, bacillus cereus, enterococus facium, listeria monocytogenes, escherichia coli, salmonella typhimurium, shigella dysenteriae, yersinia enterocolitica, aspergillus ochraceus and penicillium roqueforti. | 2007 | 17381739 |
| occurrence of ochratoxin a and ochratoxigenic mycoflora in corn and corn based foods and feeds in some south american countries. | cereals and cereal- derived products constitute the base of human and animal feeding in south american countries. this review attempts to give an overview of the ochratoxin a (ota) occurrence and potential sources of ota contamination in those products. the environmental conditions as humidity and temperature in the colonization of the substrates by aspergillus section nigri isolated from corn kernels were also discussed. the available information on the ochratoxigenic mycoflora and ota presence ... | 2007 | 17390233 |
| a comparison of chemical, antioxidant and antimicrobial studies of cinnamon leaf and bark volatile oils, oleoresins and their constituents. | the antioxidant, antifungal and antibacterial potentials of volatile oils and oleoresin of cinnamomum zeylanicum blume (leaf and bark) were investigated in the present study. the oleoresins have shown excellent activity for the inhibition of primary and secondary oxidation products in mustard oil added at the concentration of 0.02% which were evaluated using peroxide, thiobarbituric acid, p-anisidine and carbonyl values. moreover, it was further supported by other complementary antioxidant assay ... | 2007 | 17408833 |
| characterization of alcohol oxidase from aspergillus ochraceus aiu 031. | an alcohol oxidase (aod) was found from aspergillus ochraceus aiu 031, and its characteristics were revealed. this enzyme oxidized short-chain primary alcohols and ethylene glycol, and belonged to the same group as aod from methylotrophic yeast. however, it differed in the following properties. the k(m) value for ethanol was larger and that for ethylene glycol was smaller than those of aods derived from methylotrophic yeasts. the ethanol oxidation was optimal at ph 5-7 and 50-55 degrees c. the m ... | 2007 | 17726298 |
| inhibition of ochratoxin a production and growth of aspergillus species by phenolic antioxidant compounds. | the phenolic antioxidants, gallic acid, vanillic acid, protocatechuic acid, 4-hydroxybenzoic acid, catechin, caffeic acid, and chlorogenic acid were studied for their effects on ochratoxin a (ota) production and fungal growth of ochratoxigenic aspergilli. of the 12 strains tested, which included a. alliaceus, a. lanosus, a. ochraceus, a. albertensis, a. melleus, a. sulphureus, a. carbonarius, a. elegans, and a. sclerotiorum, the greatest inhibition of ota production was seen in a. sulphureus, a. ... | 2007 | 17874203 |
| [influence of the interaction of temperature and water activity on the production of ochratoxin a and the growth of aspergillus niger, aspergillus carbonarius and aspergillus ochraceus on coffee-based culture medium]. | in the present study, the effect of temperature and water activity on fungal growth and ochratoxin production on coffee-based medium was assessed. optimal growth of three aspergillus strains was observed in the same ecological conditions, namely 30 degrees c and 0.99 water activity. maximal daily growth is 11.2, 6.92, and 7.22 mm/day for aspergillus niger, aspergillus carbonarius, and aspergillus ochraceus, respectively. however, ecological conditions for optimal ochratoxin production vary accor ... | 2007 | 17898840 |
| detection and quantification of aspergillus westerdijkiae in coffee beans based on selective amplification of beta-tubulin gene by using real-time pcr. | aspergillus westerdijkiae is a new species of fungus that was recently dismembered from aspergillus ochraceus taxon. most isolates of a. westerdijkiae are able to produce large amounts of a mycotoxin called ochratoxin a (oa). oa has been found in food and beverages, such as coffee. a. westerdijkiae is very similar to a. ochraceus, and several isolates previously identified as a. ochraceus are now identified as a. westerdijkiae. by using sequences of the beta-tubulin gene, we analyzed several iso ... | 2007 | 17900727 |
| biodegradation of kerosene by aspergillus ochraceus ncim-1146. | the filamentous fungus aspergillus ochraceus ncim-1146 was found to degrade kerosene, when previously grown mycelium (96 h) was incubated in the broth containing kerosene. higher levels of nadph-dcip reductase, aminopyrine n-demethylase and kerosene biodegradation activities were found to be present after the growth in potato dextrose broth for 96 h, when compared with the activities at different time intervals during the growth phase. nadph was the preferred cofactor for enzyme activity, which ... | 2007 | 17910104 |
| [indoor moulds: results of the environmental study in office rooms]. | the aim of the study was to quantitatively and qualitatively identify moulds occurring in the air of selected office rooms. | 2007 | 18421934 |
| study of mixed function oxidase system in aspergillus ochraceus (ncim 1146). | aspergillus ochraceus (ncim-1146) has shown the ability to degrade cholesterol, camphor and naphthalene, when 96 h grown mycelium incubated in medium containing these organic compounds. presence of higher level of electron transport components and biotransformation enzyme activity were observed in aspergillus ochraceus, when grown in potato dextrose medium for 96 h. the enzyme activity preferred nadph as a cofactor and shows inhibition in the presence of co, indicating cytochrome p-450 mediated ... | 2007 | 23100682 |
| mould germination: data treatment and modelling. | the objectives of this study were i/ to examine germination data sets over a range of environmental conditions (water activity, temperature) for eight food spoilage moulds, ii/ to compare the ability of the gompertz equation and logistic function to fit the experimental plots, iii/ to simulate germination by assessing various distributions of the latent period for germination amongst a population of spores. data sets (percentage germination, p (%), versus time, t) of aspergillus carbonarius, asp ... | 2007 | 17188772 |
| biodegradation of reactive blue-25 by aspergillus ochraceus ncim-1146. | the present study dealt with the decolorization and degradation of textile dye reactive blue-25 (0.1gl(-1)) by mycelium of aspergillus ochraceus ncim-1146. spectrophotometric and visual examinations showed that the decolorization was through fungal adsorption, followed by degradation. shaking condition was found to be better for complete and faster adsorption (7h) and decolorization (20 days) of dye reactive blue-25 (100mgl(-1)) as compared to static condition. presence of glucose in medium show ... | 2007 | 17204422 |
| determination of ochratoxin a in maize bread samples by lc with fluorescence detection. | ochratoxin a (ota) is a secondary fungal metabolite produced by several moulds, mainly by aspergillus ochraceus, a. carbonarius, a. niger and by penicillium verrucosum. the present work shows the results of comparative studies using different procedures for the analysis of ota in maize bread samples. the studied analytical methods involved extraction with different volumes of pbs/methanol, different extraction apparatus, and clean-up through immunoaffinity columns. the separation and identificat ... | 2007 | 19073023 |
| [anti-bacterial activity of extracts from fungi collected from mangrove rhizophora mangle (rhizophoraceae) roots in venezuela]. | the antibacterial activity of marine fungi extracts was evaluated by a test of efficiency. the fungi were previously inoculated in malt agar (ema) extract with 50% of seawater and growth for 60 days. triplicate antibiograms were carried out with the extracts. an anova i with a posteriori duncan test were applied to the diameters of inhibition zones. the extracts of aspergillus ochraceus 3mcmc3 and penicillium citrinum (14) 4mcmc16 present wide spectral antibacterial properties, inhibiting 100% a ... | 2007 | 19086381 |
| genomic characterization of a novel partitivirus infecting. aspergillus ochraceus. | three double-stranded rna (dsrna) segments from aspergillus ochraceus isolate fa 0611, designated as aor1, aor2, and aor3, were cloned and sequenced. aor1 was identical with aov dsrna 1 previously reported from a. ochraceus atcc 28706, which putatively encoded an rna-dependent rna polymerase of an unidentified mycovirus. aor2 was found to encode a putative viral capsid protein (cp) with 63% similarity to that of penicillium stoloniferum virus s, which was detected from p. stoloniferum atcc 14586 ... | 2008 | 18770020 |
| identification of the antimicrobial substances produced by solanum palinacanthum (solanaceae). | to find out natural antimicrobial agents as alternative in therapeutics and to preserve food, the methanol extract of solanum palinacanthum aerial parts was submitted to purification steps guided by antibacterial and antifungal assays. as a consequence, the flavonoid rutin and 3,5-dicaffeoylquinic acid were isolated by column chromatography and high performance liquid chromatography, and identified by mass and nuclear magnetic resonance spectrometry. minimal inhibitory concentrations (mic) of th ... | 2008 | 18797795 |
| [isolation and fermentation condition of milk-clotting enzyme producing strain from glutinous rice wine]. | glutinous rice wine is a traditional food in south of china and it can coagulate milk. it has been proved that its function of coagulating milk is because of the presence of milk-clotting enzyme produced by microorganisms in glutinous rice wine. the aim of this work is to isolate milk-clotting enzyme producing strain from glutinous rice wine and study the fermentation condition. we screened out four bacteria and fungus by gradient dilution. it was proved that mold played the most important role ... | 2008 | 18807982 |
| study of the ochratoxin a effect on aspergillus parasiticus growth and aflatoxin b1 production. | aflatoxin b1 (afb1) is a carcinogenic metabolite produced by certain aspergillus species. ochratoxin a (ota) is a metabolite of aspergillus ochraceus and penicillium verrucosum. afb1 and ota are amongst the most frequent combinations of mycotoxins found in plant products. thus, synergistic effects or interactions between the two mycotoxins could be taking place. the aim of the present study was to investigate the effect of ota on aspergillus parasiticus growth and afb1 production in yeast extrac ... | 2008 | 18471949 |
| corncob-induced endo-1,4-beta-d-xylanase of aspergillus oryzae mtcc 5154: production and characterization of xylobiose from glucuronoxylan. | eight different fungi were cultivated in a peptone-yeast extract medium containing 1% oat spelt xylan (osx) to evaluate endo-1,4-beta-xylanase secretion for xylooligosaccharide (xos) production. aspergillus oryzae mtcc 5154, aspergillus flavus , aspergillus niger , and aspergillus ochraceus showed significant titers of endoxylanases, which were further used for the production of xos from birch wood xylan (bwx). a. oryzae produced 89.5 +/- 1.13% xos in the hydrolysate at 24 h of reaction. the eff ... | 2008 | 18489109 |
| effect of ochratoxin a on redox-regulated transcription factors, antioxidant enzymes and glutathione-s-transferase in cultured kidney tubulus cells. | ochratoxin a (ota), a mycotoxin mostly produced by aspergillus ochraceus and penicillium verrucosum, is a worldwide contaminant of food and feedstuff. ota is nephrotoxic and a renal carcinogen in rodents. the underlying molecular and cellular mechanisms by which ota exhibits its toxicity have yet not been fully clarified. in the present study the effects of ochratoxin a on the activity of redox-regulated transcription factors, antioxidant enzymes, as well as glutathione-s-transferase (gst) have ... | 2008 | 18547704 |
| detection of aspergillus spp. and aflatoxin b1 in rice in india. | twelve hundred rice samples consisting of paddy (675) and milled rice (525) were collected from 20 states across india. these samples were assessed for aspergillus spp. infection on selective medium and aflatoxin b(1) (afb1) by indirect competitive elisa. in this investigation, aspergillus flavus contamination dominated in all the seed samples. the other major contaminants were aspergillus niger, aspergillus ochraceus and aspergillus parasiticus. out of 1200 rice samples, 67.8% showed afb1 rangi ... | 2009 | 19028301 |
| moulds and ochratoxin a on surfaces of artisanal and industrial dry sausages. | the use of moulds as a seasoning for sausage can have both desirable and undesirable consequences. the desirable consequences are the creation of a successful product that appeals to consumers. the undesirable consequences are due to the growth of undesirable moulds that produce highly toxic secondary metabolites referred to as mycotoxins. the aim of the paper was to investigate the presence of moulds producing ochratoxin a (ota) on the surface of sausages from northern italy. a total of 757 mou ... | 2009 | 19028307 |
| its-based detection and quantification of aspergillus ochraceus and aspergillus westerdijkiae in grapes and green coffee beans by real-time quantitative pcr. | aspergillus ochraceus and a. westerdijkiae are considered the most important ochratoxin a (ota) producing species included in aspergillus section circumdati which contaminate foodstuffs and beverages for human consumption. in this work a real-time quantitative pcr protocol was developed to detect both species using sybr green and primers designed on the basis of the multicopy its1 region of the rdna. the assay had high efficiency (94%) and showed no inhibition by host or fungal dna other than th ... | 2009 | 19268380 |
| food preservative potential of essential oils and fractions from cymbopogon citratus, ocimum gratissimum and thymus vulgaris against mycotoxigenic fungi. | the food preservative potential of essential oils from three aromatic plants cymbopogon citratus, ocimum gratissimum and thymus vulgaris and their fractions was investigated against two mycotoxigenic strains each of aspergillus ochraceus, penicillium expansum and p. verrucosum. the fungicidal activity was determined and expressed as a number of decimal reduction of the colony forming units per ml (ndr cfu). the influence of ph variation on this activity was studied. the ndr cfu varied with the e ... | 2009 | 19268382 |
| xylanases from aspergillus niger, aspergillus niveus and aspergillus ochraceus produced under solid-state fermentation and their application in cellulose pulp bleaching. | this study describes the production of xylanases from aspergillus niveus, a. niger, and a. ochraceus under solid-state fermentation using agro-industrial residues as substrates. enzyme production was improved using a mixture of wheat bran and yeast extract or peptone. when a mixture of corncob and wheat bran was used, xylanase production from a. niger and a. ochraceus increased by 18%. all cultures were incubated at 30 degrees c at 70-80% relative humidity for 96 h. for biobleaching assays, 10 o ... | 2009 | 19271244 |
| effect of ozone on spore germination, spore production and biomass production in two aspergillus species. | the ability of ozone gas to reduce food spoilage is relatively well documented, but the developmental effects of the gas on food spoilage fungi are not well known. in this study two model aspergilli, aspergillus nidulans and aspergillus ochraceus were used to study the effects of ozone on spore germination, sporulation and biomass production. these effects were examined under three levels of ozone; two high level ozone exposures (200 and 300 micromol mol(-1)) and one low level exposure (0.2 micr ... | 2009 | 19533409 |
| characterization of the aspergillus ochraceoroseus aflatoxin/sterigmatocystin biosynthetic gene cluster. | production of carcinogenic aflatoxins has been reported from members of aspergillus section flavi, aspergillus section nidulantes and a newly proposed aspergillus section ochraceorosei that consists of aspergillus ochraceoroseus and a. rambellii. unlike members of section flavi, a. ochraceoroseus and a. rambellii have been shown to accumulate both aflatoxin (af) and the aflatoxin precursor sterigmatocystin (st). alhough morphologically distinct from a. nidulans, molecular characterization of a. ... | 2009 | 19537208 |
| mycobiota and concentration of ochratoxin a in concentrated poultry feed from venezuela. | the main objective of this study was to evaluate the frequency distribution of mycobiota and the concentration of ochratoxin a (ota) in 50 samples from one company of commercial brand poultry feed produced in venezuela. the concentration of ota in the samples analyzed was determined using the competitive elisa method. the most frequently isolated genera of moulds were aspergillus (36%) and penicillium (20%). of these genera, the most frequently isolated species were aspergillus flavus, aspergill ... | 2009 | 19580016 |
| the effect of mycotoxins found in some herbal plants on biochemical parameters in blood of female albino mice. | in this study, twenty five samples ofwell-known herbs in riyadh, saudi arabia were collected and analyzed for total fungi count (tfc). mycotoxins were extracted and screened using smky liquid medium. one hundred and thirty adult female albino mice were grouped into three wherein one group (n = 110) was fed with an aqueous extract from herbal plants. the second group (n = 15) was fed with an aqueous extract of the isolated fungal species. the third group comprised the control group which was give ... | 2009 | 19634489 |
| analysis of the effect of nutritional factors on ota and otb biosynthesis and polyketide synthase gene expression in aspergillus ochraceus. | the effect of a wide variety of nutritional based biotic factors on the production of both ota and otb biosynthesis in a. ochraceus was assessed. different carbon sources including glucose, sucrose, maltose, galactose, xylose and glycerol appear to repress ota production when the fungus is grown in ota permissive pdy medium. in contrast lactose appears to induce ota production, with the addition of lactose and galactose to the ota restrictive pdc medium resulting in marked increases in ota level ... | 2009 | 19682762 |
| effect of processing on ochratoxin a content in dried beans. | dried pink beans naturally contaminated with ochratoxin a (ota) and dried carioca beans artificially contaminated with ota by inoculation with aspergillus ochraceus (atcc 22947) were tested for ochratoxin a levels as follows: dried beans were washed with water for 2, 60 or 120 min, soaked in water for 60, 120 min or 10 h, and cooked for 60 or 120 min. at each step, test water and beans were separated. test water, raw beans and cooked beans were analyzed for ota. the amount of ota partitioned int ... | 2009 | 19693718 |
| discrimination of the main ochratoxin a-producing species in aspergillus section circumdati by specific pcr assays. | ochratoxin a (ota) is one of the most important mycotoxins because of its high toxicity to both humans and animals and its occurrence in a number of basic foods and agro-products. some of the main ota-producing species belong to aspergillus section circumdati, whose taxonomy has been lately revised with the description of new species. the high morphological similarity of these species (aspergillus ochraceus, aspergillus steynii and aspergillus westerdijkiae) makes difficult discrimination among ... | 2009 | 19819579 |
| intracellular biogenic silver nanoparticles for the generation of carbon supported antiviral and sustained bactericidal agents. | intracellular silver nanoparticles produced by exposing silver ions to the fungus aspergillus ochraceus were heat-treated in nitrogen environment to yield silver nanoparticles embedded in carbonaceous supports. this carbonaceous matrix embedded silver nanoparticles showed antimicrobial properties against both bacteria (gram-positive and gram-negative) and virus (m 13 phage virus). the bactericidal effects were noticed even after washing and repeated exposure of these carbon supported silver nano ... | 2009 | 19746940 |
| mycotoxic nephropathy in bulgarian pigs and chickens: complex aetiology and similarity to balkan endemic nephropathy. | spontaneous nephropathy in bulgaria, which is observed frequently during meat inspection and which differs morphologically from the classical description of mycotoxic porcine/chicken nephropathy as made in denmark, was found to have a multi-mycotoxic aetiology being mainly provoked by a combined effect of ochratoxin a, penicillic acid and fumonisin b1 in addition to a not-yet-known metabolite. mean contamination levels of ochratoxin a were consecutively low (188.8 and 376.4 microg kg(-1)) in con ... | 2010 | 19753495 |
| coumarin metabolic routes in aspergillus spp. | coumarin metabolism by several aspergillus strains was studied. aspergillus ochraceus and aspergillus niger carried out the reduction of the c3-c4 double bond to yield dihydrocoumarin in 24h. meanwhile, the first strain did not transform dihydrocoumarin after 7d, a. niger demonstrated to have two divergent catabolic pathways: (a) the lactone moiety opening and further reduction of the carboxylic acid furnishing the primary alcohol 2-(3-hydroxypropyl)phenol and, (b) the hydroxylation of the aroma ... | 2010 | 21354531 |
| modelling the effect of temperature and water activity in the growth boundaries of aspergillus ochraceus and aspergillus parasiticus. | the aim of this work was to model the growth of aspergillus parasiticus and aspergillus ochraceus, both mycotoxin producers, near to the growth/no growth boundaries and validate those models in sterile maize grain, peanuts and coffee beans. malt extract agar was adjusted to six different water activities: 0.93, 0.91, 0.89, 0.87, 0.85 and 0.80. plates were incubated at 10, 15, 20, 25, 30, 37 and 42 °c. for each of the 42 conditions, 10 petri dishes were inoculated. both kinetic and probability mo ... | 2010 | 21356445 |
| isolation and identification of microbes associated with mobile phones in dammam in eastern saudi arabia. | this study was conducted to determine microbial contamination of mobile phones in the city of dammam, in the eastern region of saudi arabia, and identify the most important microbial species associated with these phones in order to take the necessary remedial measures. | 2010 | 22022665 |
| production and properties of xylanases from aspergillus terricola marchal and aspergillus ochraceus and their use in cellulose pulp bleaching. | aspergillus terricola and aspergillus ochraceus, isolated from brazilian soil, were cultivated in vogel and adams media supplemented with 20 different carbon sources, at 30 degrees c, under static conditions, for 120 and 144 h, respectively. high levels of cellulase-free xylanase were produced in birchwood or oat spelt xylan-media. wheat bran was the most favorable agricultural residue for xylanase production. maximum activity was obtained at 60 degrees c and ph 6.5 for a. terricola, and 65 degr ... | 2010 | 20091051 |
| lipoperoxidation affects ochratoxin a biosynthesis in aspergillus ochraceus and its interaction with wheat seeds. | in aspergillus nidulans, aspergillus flavus, and aspergillus parasiticus, lipoperoxidative signalling is crucial for the regulation of mycotoxin biosynthesis, conidiogenesis, and sclerotia formation. resveratrol, which is a lipoxygenase (lox) and cyclooxygenase inhibitor, downmodulates the biosynthesis of ochratoxin a (ota) in aspergillus ochraceus. in the genome of a. ochraceus, a lox-like sequence (aoloxa; national center for biotechnology information (ncbi) accession number: dq087531) for a l ... | 2010 | 20101489 |
| fungal growth inhibition of regenerated cellulose nanofibrous membranes containing quillaja saponin. | antifungal properties were introduced in nonwoven regenerated cellulose (rc) nanofibrous membrane using quillaja saponin. to generate cellulose membranes, deacetylation of electrospun cellulose acetate (ca) nanofibrous membranes was performed using 0.05 m naoh and ethanol for membranes both loaded and unloaded with quillaja saponin. chemical and physical properties of nonwoven ca and rc nanofibrous membrane were characterized using scanning electron microscopy, attenuated total reflectance-fouri ... | 2010 | 20213194 |
| vice to virtue: intracellular biogenic nanoparticles for the generation of carbon supported catalysts. | intracellular biogenic nanoparticles are considered disadvantageous as the separation of the nanoparticles from the biomass becomes intricate. however realizing the importance of carbon supported catalyst for many important organic reactions we envisaged these nanoparticles as a source for carbon supported catalyst. herein we demonstrate the heat treatment of intracellular biogenic nanoparticles under inert atmosphere as an efficient method for the preparation of carbon supported metal oxide cat ... | 2010 | 20352735 |
| chronic monolateral otomycosis in a dog caused by aspergillus ochraceus. | aspergillus ochraceus, a widely distributed filamentous fungus, was isolated and identified by cytology and culture as the cause of unilateral ceruminous purulent otitis in a 4-year-old male mixed-breed dog. the pathogenic role of the fungal isolate was confirmed by a good response to antifungal therapy and the absence of other pathogens. no underlying diseases were identified and the dog recovered after 3 weeks of therapy with oral itraconazole and topical miconazole. | 2010 | 20409075 |
| antifungal effects of the bioactive compounds enniatins a, a(1), b, b(1). | to produce enniatin (ens), fusarium tricinctum cect 20150 was grown in a liquid medium of potato (pdb), being mycotoxin purified by high performance liquid chromatography (hplc) with a reverse phase semipreparative column using a mobile phase of acetonitrile/water using gradient condition. the purity of the ens fractions was verified by analytical hplc and lc/ms-ms. the pure fractions of ens were utilized to study the biological activity on several mycotoxigenic moulds as fusarium verticilloides ... | 2010 | 20417654 |
| growth inhibition of aspergillus ochraceus zmpbf 318 and penicillium expansum zmpbf 565 by four essential oils. | fungi produce a large variety of extracellular proteins, organic acids, and other metabolites and can adapt to several environmental conditions. mycotoxin-producing moulds of the genera aspergillus and penicillium are common food contaminants. one of the natural ways to protect food from mould contamination is to use essential oils. in this study, we evaluated the effect of essential oils of cinnamon, lavender, rosemary, and sage at 1 % (v/v) concentration in yeast media inoculated with spores ( ... | 2010 | 20587393 |
| impact of drying surface and raking frequencies on mold incidence, ochratoxin a contamination, and cup quality during preparation of arabica and robusta cherries at the farm level. | the aim of this study was to evaluate the impact and contribution of various drying surfaces (soil, cement, and tarpaulin) and raking frequencies (1 and 4/day) on the incidence of toxigenic molds, ochratoxin a (ota) production, and on the overall cup quality during preparation of arabica and robusta coffee cherry in india. two individual experimental batches (run 1 at the begin of harvest and run 2 at the end of harvest) were set up for the study. results showed high incidence of molds in coffee ... | 2010 | 20618072 |
| coffee berry borer (hypothenemus hampei)—a vector for toxigenic molds and ochratoxin a contamination in coffee beans. | coffee berry borer (cbb, hypothenemus hampei ferrari) is a common insect pest in coffee plantations and is a suspected vector of various mycotoxin-producing molds. in the present study, field trials were undertaken consecutively for 3 years to evaluate the impact of cbb on the microbial contamination of arabica and robusta coffee bean varieties, with emphasis laid toward ochratoxin a (ota)-producing fungi. results revealed higher microbial contamination in cbb-infested beans in both the varietie ... | 2010 | 20618085 |
| lifetime, low-dose ochratoxin a dietary study on renal carcinogenesis in male fischer rats. | carcinoma arising from male rat renal parenchyma is an aspect of the nephrotoxicity of ochratoxin a (ota) and is a factor in considering application of animal data to human health risk assessment. we present experimental data to complement already published and to complete dose-response findings for dietary ota. from 34 rats, only four unilateral renal carcinomas (12%) developed during a 2-year exposure to dietary ota, contaminated to give the same weekly overall dosage as in the 50 µg kg(-1) ga ... | 2010 | 20694869 |
| screening of filamentous fungi to identify biocatalysts for lupeol biotransformation. | the goal of the study was to evaluate the ability of filamentous fungi to biotransform the pentacyclic triterpene lupeol. the microbial transformations were carried out in shake flasks in different media. experiments were also run with control flasks. samples of each culture were taken every 24 hours, extracted with ethyl acetate, and analyzed by gc-ms. the biotransformation of lupeol by aspergillus ochraceus and mucor rouxii afforded two compounds in each culture, which were detected in the cul ... | 2010 | 20877212 |
| [effects of environmental stress on secondary metabolites of aspergillus ochraceus lcj11-102 associated with the coral dichotella gemmacea]. | to explore the secondary metabolites of fungus aspergillus ochraceus lcj11-102 associated with the coral dichotella gemmacea under environmental stress and to obtain characteristic compounds with biological activities. | 2010 | 20931869 |
| 7-nor-ergosterolide, a pentalactone-containing norsteroid and related steroids from the marine-derived endophytic aspergillus ochraceus en-31. | 7-nor-ergosterolide (1), a rare 7-norsteroid with an unusual pentalactone b-ring system, and two new steroid derivatives, 3β,11α-dihydroxyergosta-8,24(28)-dien-7-one (2) and 3β-hydroxyergosta-8,24(28)-dien-7-one (3), were characterized from the culture extract of aspergillus ochraceus en-31, an endophytic fungus isolated from the marine brown alga sargassum kjellmanianum. in addition, nine known related steroids were isolated and identified. the structures of these compounds were established on ... | 2010 | 21043476 |
| culture-independent characterization of bacteria and fungi in a poultry bioaerosol using pyrosequencing: a new approach. | work in animal production facilities often results in exposure to organic dusts. previous studies have documented decreases in pulmonary function and lung inflammation among workers exposed to organic dust in the poultry industry. bacteria and fungi have been reported as components of the organic dust produced in poultry facilities. to date, little is known about the diversity and concentration of bacteria and fungi inside poultry buildings. all previous investigations have utilized culture-base ... | 2010 | 21058154 |
| essential oil of thymus capitatus hoff. et link. from matmata, tunisia: gas chromatography-mass spectrometry analysis and antimicrobial and antioxidant activities. | thymus capitatus hoff. et link. essential oil was constituted by thymol (89.06%) as a major component followed by p-cimene (5.04%) and γ-terpinene (3.19%) after analysis by gas chromatography-flame ionization detection and gas chromatography-mass spectrometry. the antioxidant activity assays of the essential oil used in the inhibition of the radical cation 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonate) and the free radical 2,2-diphenyl-1-picrylhydrazyl showed high 50% inhibitory concentration ... | 2010 | 21091258 |
| modified magnetic nanoparticles in an electrochemical method for the ochratoxin a determination in vitis vinifera red grapes tissues. | this work described the development and characterization of an electrochemical method using square wave voltammetry (swv) combined with the use of modified magnetic nanoparticles (mnps), which had shown a rapid and sensitive determination of ochratoxin a (ota) in wine grapes (cabernet sauvignon, malbec and syrah) post-harvest tissues. the wine grapes were inoculated with aspergillus ochraceus to obtain ota in artificially infected samples. the ota was directly determined using square-wave voltam ... | 2010 | 21111187 |
| production and characterization of a milk-clotting enzyme from aspergillus oryzae mtcc 5341. | microbial milk-clotting enzymes are valued as calf rennet substitutes in the cheese industry. aspergillus oryzae mtcc 5341 was identified to produce the highest milk-clotting activity during screening of 16 fungal strains. solid state fermentation using wheat bran along with 4% defatted soy flour and 2% skim milk powder as substrate was optimal for growth of a. oryzae and production of the enzyme. nearly 40,000 u/g bran of milk-clotting activity was present at the end of 120 h. the enzyme could ... | 2010 | 19727708 |
| aflatoxins and ochratoxin a in pistachios sampled in spain: occurrence and presence of mycotoxigenic fungi. | a survey of the fungal contamination and occurrence of aflatoxins and ochratoxin a (ota) in 50 pistachio nut samples collected from commercial stores around catalonia (spain) was carried out. aspergillus flavus was found in 30% of samples, whereas aspergillus section nigri, aspergillus ochraceus, and penicillium verrucosum were found in 40%, 2% and 26% of samples, respectively. a total of 204 fungal isolates were obtained; 70.8% of a. flavus isolates were able to produce aflatoxin b1 and b2, whe ... | 2010 | 24779573 |