Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| establishment and characterization of a thymidine kinase deficient avian fibroblast cell line derived from a japanese quail cell line, qt35. | an avian thymidine kinase deficient (tk-) fibroblast cell line (qttk-) was established from a japanese quail cell line, qt35, and characterized the biological properties. qttk- could grow in the presence of 5-bromo-2'-deoxyuridine (budr, 100 micrograms/ml) and not in the growth medium with hypoxanthine-aminopterin-thymidine. compared to qt35 cells, the 3h-thymidine incorporation of the qttk- cells and the tk activity of the cell extract significantly decreased to 0.3% and 0.5%, respectively. in ... | 1991 | 1832032 |
| effect of aflatoxin b1 on the efficacy of turkey herpesvirus vaccine against marek's disease. | the effect of feeding aflatoxin b1 (afb1) (0.5 ppm) was studied in young chicks. the frequency and the severity of gross and microscopic lesions of marek's disease were significantly higher in those birds which had been vaccinated with turkey herpesvirus (hvi) and birds challenged with marek's disease virus which had been given afb1 in the feed than in those given normal feed. the protective efficacy of hvt vaccine, as judged on the basis of gross and histopathological lesions, was 86.1 and 77.3 ... | 1991 | 1910199 |
| replication of marek's disease virus in chicken feather tips containing vaccinal turkey herpesvirus dna. | the presence of herpesvirus of turkeys (hvt) dna in the feather tips of chickens vaccinated with hvt was assessed by dot blot hybridisation with a probe specific for hvt and lacking homology to mdv dna. only small amounts of hvt dna were detected in the feather tips of chickens that were vaccinated or left in contact with hvt vaccinated chickens. however when chickens were challenged with virulent mdv, hvt dna was detected in the feather tips of vaccinated chickens and the largest amount was det ... | 1991 | 18679997 |
| marek's disease virus isolates with unusual tropism and virulence for ocular tissues: clinical findings, challenge studies and pathological features. | outbreaks of marek's disease (md) were diagnosed in two flocks from the same company. clinical signs, mainly blindness (>90%), but also depression, mild paralysis, and 11 to 12% mortality by 20 weeks of age were observed. md virus, serotype 1 was isolated. the isolates were designated nc-1 (flock 1) and nc-2 (flock 2). challenge experiments were conducted with these isolates and with two reference md virus strains (jm/102w and md5) in unvaccinated, turkey herpesvirus- (hvt) vaccinated and bivale ... | 1991 | 18680042 |
| protection against marek's disease by a fowlpox virus recombinant expressing the glycoprotein b of marek's disease virus. | fowlpox virus (fpv) recombinants expressing the glycoprotein b and the phosphorylated protein (pp38) of the ga strain of marek's disease virus (mdv) were assayed for their ability to protect chickens against challenge with virulent mdv. the recombinant fpv expressing the glycoprotein b gene elicited neutralizing antibodies against mdv, significantly reduced the level of cell-associated viremia, and, similar to the conventional herpesvirus of turkeys, protected chickens against challenge with the ... | 1992 | 1310755 |
| detection of infectious laryngotracheitis virus infected cells with cloned dna probes. | a genomic library of infectious laryngotracheitis virus (iltv) dna bamh1 fragments was prepared and two cloned fragments were evaluated for their potential as probes for the detection of iltv infected cells. the virus was purified by a modified sucrose density gradient procedure for the isolation of pure iltv dna. a genomic library was constructed using bamh1-digested iltv dna and pgem7 as a vector. a 1.1 kb cloned bamh1 fragment of iltv dna was tested in a slot or dot blot assay for the detecti ... | 1992 | 1316798 |
| expression of the marek's disease virus homolog of herpes simplex virus glycoprotein b in escherichia coli and its identification as b antigen. | marek's disease (md) is an oncogenic disease of chickens caused by md virus (mdv). among the major glycoproteins found in mdv-infected cells are gp100, gp60, and gp49, detected by immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis with antisera previously shown to be reactive with b antigen in immunodiffusion analysis. following treatment with tunicamycin (tm), an inhibitor of n-linked glycosylation, the same sera were reported to detect two molecules call ... | 1992 | 1318409 |
| influence of turkey herpesvirus vaccination on the b-haplotype effect on marek's disease resistance in 15.b-congenic chickens. | eight recently developed 15.b congenic lines of chickens were tested for marek's disease (md) resistance by intra-abdominal injection of cell-associated preparations of md virus of a virulent strain (jm), a very virulent strain (md5), or md5 after vaccination with turkey herpesvirus (hvt) strain fc126. chickens of the 15.n congenic line (b15b21 or b21b21) were very resistant to jm-induced md, in contrast to chickens homozygous for the b-haplotypes 2, 5, 12, 13, 15, or 19. after md5 infection, mo ... | 1992 | 1320867 |
| differentiation of oncogenic and nononcogenic strains of marek's disease virus type 1 by using polymerase chain reaction dna amplification. | differentiation of oncogenic and nononcogenic strains of marek's disease virus type 1 (mdv1) was attempted by polymerase chain reaction (pcr) using the primers chosen from the sequence within the long inverted repeats of mdv1 dna. pcr of the dnas extracted from oncogenic-strain-infected cells and marek's disease tumor cell lines produced a major product containing two or three copies of 132-base-pair (bp) repeat units, whereas pcrs of the dnas extracted from nononcogenic-strain-infected cells yi ... | 1992 | 1329711 |
| antigen b of the vaccine strains of marek's disease virus and herpesvirus of turkeys presents heat-labile group and serotype specific epitopes. | antigen b of marek's disease virus (mdv) vaccine strains cvi988 and sb1 (serotypes 1 and 2) and herpesvirus of turkeys (hvt) (serotype 3) is formed of oligomeric molecules that are detergent-stable and heat-labile. immunoblots of native membranal extracts of hvt- and mdv-infected chick embryo fibroblasts (cef) probed with avian monoserotypic antisera, murine monoclonal antibodies (mab) to the three serotypes and mab to antigen b showed two distinct patterns of high molecular weight oligomeric an ... | 1992 | 1333751 |
| [pathologic changes in the organs in chickens after infection with the turkey herpesvirus thv-bio-i]. | the objective of our work was to investigate the dynamics of pathological lesions of chicken organs after infection with high doses of turkey herpesvirus thv-bio-i. this virus strain is commonly used in form of the marvak vaccine against marek's disease of poultry in czechoslovakia. high doses of the vaccine are used in practice with respect to the epizootological situation. the incidence of pathological lesions in the organs of brown leghorn chickens was investigated in a five-week experiment. ... | 1992 | 1336236 |
| protection of chickens from newcastle and marek's diseases with a recombinant herpesvirus of turkeys vaccine expressing the newcastle disease virus fusion protein. | recombinant strains of herpesvirus of turkeys (hvt) were constructed that contain either the fusion protein gene or the hemagglutinin-neuraminidase gene of newcastle disease virus (ndv) inserted into a nonessential gene of hvt. expression of the ndv antigens was regulated from a strong promoter element derived from the rous sarcoma virus long terminal repeat. recombinant hvt strains were stable and fully infectious in cell culture and in chickens. chickens receiving a single intra-abdominal inoc ... | 1992 | 1485872 |
| effects of age at infection with serotype 2 marek's disease virus on enhancement of avian leukosis virus-induced lymphomas. | inoculation of susceptible, 15i5 x 7i chickens with serotype 2 marek's disease virus (mdv) at various ages had no influence on the development of avian leukosis virus (alv)-induced viraemia or antibody in chickens infected with alv and turkey herpesvirus (hvt) at hatch. however, the incidence of alv-induced lymphoma (ll) was significantly higher in chickens infected with alv and hvt at hatch and inoculated with serotype 2 mdv up to 6 weeks of age than in chickens receiving serotype 2 mdv at 8 to ... | 1993 | 18671041 |
| cosmid library of the turkey herpesvirus genome constructed from nanogram quantities of viral dna associated with an excess of cellular dna. | a protocol was designed for the rapid and efficient construction of cosmid libraries from cell-associated viral genomes available in very low quantities. purification of viral dna from cellular dna was unnecessary. the vast excess of cellular dna compensated for the limited amount of available viral dna, enabling titration of the restriction endonuclease partial digest. a cosmid library of the turkey herpesvirus dna genome was constructed from 1.5 micrograms of cellular dna containing approximat ... | 1993 | 8097201 |
| nucleotide sequence of the glycoprotein c (gc) homologous gene of marek's disease virus (mdv) serotype 2 and comparison of gc homologous genes among three serotypes of mdv. | the sequence analysis of the genomic region of marek's disease virus (mdv) serotype 2 (mdv2) which had homology with the gene encoding mdv serotype 1 (mdv1) homologue of glycoprotein c (gc) of herpes simplex virus revealed that there was a single open reading frame (orf) capable of encoding 479 amino acids. the predicted protein from this orf had strong hydrophobic regions in both the amino and carboxyl termini and several potential n-linked glycosylation sites, as seen in those from the orfs of ... | 1993 | 8117828 |
| efficacy in chickens of a herpesvirus of turkeys recombinant vaccine containing the fusion gene of newcastle disease virus: onset of protection and effect of maternal antibodies. | the onset of protection against newcastle disease and the effect of maternal antibodies to newcastle disease virus (ndv) and marek's disease virus (mdv) on vaccine efficacy were determined following vaccination of chickens with a recombinant herpesvirus of turkeys (hvt) vaccine expressing the fusion (f) glycoprotein gene of ndv. onset of protection following intra-abdominal administration of the recombinant hvt/f vaccine at 1 day of age and subsequent ocular challenge with the neurotropic veloge ... | 1993 | 8141730 |
| identification of genomic regions of the herpesvirus of turkeys (hvt) with helper activity for avian adeno-associated virus (aaav). | herpesvirus of turkeys (hvt) is a potent helper for the defective parvovirus avian adeno-associated virus (aaav). to study the helper mechanism at the molecular level, we established a complete cosmid library of hvt dna in a set of seven overlapping clones and transiently cotransfected secondary chicken embryo fibroblast (cef) cells with aaav dna and recombinant cosmids (cbl) (individual as well as in different combinations). using an aaav-specific indirect immunofluorescence assay, we identifie ... | 1993 | 8382409 |
| construction and properties of a turkey herpesvirus recombinant expressing the marek's disease virus homologue of glycoprotein b of herpes simplex virus. | a herpesvirus of turkeys (hvt) recombinant containing a 3.9 kbp fragment of marek's disease virus (mdv) dna encoding mdv glycoprotein b (gb), stably integrated into the thymidine kinase (tk) gene of hvt, has been constructed. the replication of the recombinant in chick embryo fibroblasts (cef) was comparable to that of wild-type hvt. the recombinant expressed authentic mdv gb and its processed forms (110k, 65k and 48k) in cef as shown by immunoblotting using an mdv-specific anti-peptide serum. n ... | 1993 | 8383172 |
| avian herpesvirus as a live viral vector for the expression of heterologous antigens. | control of marek's disease in the poultry industry has been successfully achieved for several decades by large-scale vaccination of day-old chickens with live herpesvirus of turkeys (hvt) strains. several features of this virus including lack of pathogenicity and long-term immune protection due to a persistent viraemic infection made us decide to use hvt as a live viral vector for the expression of foreign antigens. potential sites for the integration of foreign dna in the unique short region of ... | 1993 | 8383385 |
| identification and sequence analysis of the homologues of the herpes simplex virus type 1 glycoprotein h in marek's disease virus and the herpesvirus of turkeys. | the glycoprotein h (gh) genes of two avian herpesviruses, marek's disease virus and the herpesvirus of turkeys, have been cloned and sequenced and the coding regions found to be of 2439 and 2424 nucleotides respectively. the predicted primary polypeptide products of these open reading frames are 813 and 808 amino acids and correspond to mrs of 90,800 and 91,100. both amino acid sequences exhibit characteristic glycoprotein features such as hydrophobic signal and anchor sequences and potential si ... | 1993 | 8389802 |
| development of pcr method specific for marek's disease virus. | a rapid polymerase chain reaction (pcr) assay specific for marek's disease virus (mdv) was developed. this assay was able to detect mdv in inoculated chick kidney cells at dilutions of 10(-5). negative pcr results were obtained using uninoculated chick cells, marek's disease vaccine (sb), herpesvirus of turkeys (hvt) and fowl laryngotracheitis vaccine (lt). bursae, feathers and kidneys from mdv infected chickens were positive in the pcr assay. the same tissues from normal chickens were negative. ... | 1993 | 8391640 |
| selection of marek's disease virus recombinants expressing the escherichia coli gpt gene. | we developed a positive selection method for recovering marek's disease virus (mdv) recombinants. the escherichia coli xanthine-guanine phosphoribosyltransferase gene (gpt), under the control of the major immediate-early promoter from cytomegalovirus, was inserted into the inverted repeats flanking the unique long (ul) region of a non-pathogenic serotype 2 mdv strain 281mi/1. in a second demonstration of the usefulness of the positive selection system, the gpt gene was inserted into the inverted ... | 1993 | 8393240 |
| the complete sequence and gene organization of the short unique region of herpesvirus of turkeys. | the dna sequence of the whole of the short unique region (u(s)) and that of part of the short terminal repeat (trs) of herpesvirus of turkeys (hvt) were determined. hvt u(s) is 8.6 kbp long and contains eight potential open reading frames (orfs). seven of these have counterparts in the u(s) of herpes simplex virus type 1 (hsv-1). the homologous proteins include us1, us2, us10, protein kinase (us3) and the glycoproteins gd, gi and ge. in addition, hvt contains one orf which has a counterpart in t ... | 1993 | 8409940 |
| cosmid cloning and restriction endonuclease mapping of the herpesvirus of turkeys (hvt) genome. | the genomic libraries of the herpesvirus of turkeys (hvt) presented to date do not include the entire genome. to construct a complete genomic library, hvt dna was partially digested with sau3a and inserted into the double cos site vector pcos2embl. the psti maps derived from the partial sau3a library demonstrate that the left region of the genome compared to the right area is overrepresented. similar to the libraries of the hvt genome established earlier, a defined portion of the middle genomic ... | 1994 | 8198443 |
| b haplotype influence on the relative efficacy of marek's disease vaccines in commercial chickens. | the objectives were to investigate whether or not b haplotypes influence vaccinal immunity against marek's disease (md) in commercial chickens and to evaluate whether retrospective analysis would detect the influence. this method involved evaluating the b haplotypes of turkey herpesvirus (hvt)-vaccinated sick vs normal chickens from a flock afflicted with md symptoms. an analysis of the retrospective data disclosed that md symptoms were present in a higher proportion of b2b19 than b2b21 chickens ... | 1994 | 8202426 |
| partial inhibition by turkey herpesvirus of serotype 2 marek's disease virus plaque formation and in vivo infectivity. | the increased use of serotype 2 marek's disease virus (mdv) and serotype 3 turkey herpesvirus (hvt) as components of effective bivalent vaccines against marek's disease (md) prompted studies on the possible interactions of these two viruses in vitro and in vivo. the replication of the sb-1 strain of mdv was compared with replication of the fc126/2 strain of hvt in chickens and cell cultures infected with one or both viruses. replication of mdv was reduced in the presence of hvt in both in vitro ... | 1994 | 7702514 |
| syngeneic lysis of reticuloendotheliosis virus-transformed cell lines transfected with marek's disease virus genes by virus-specific cytotoxic t cells. | cell-mediated immune responses against marek's disease virus (mdv) antigens were examined using reticuloendotheliosis virus (rev)-transformed cell lines of two haplotypes (b19b19 and b13b13). these cell lines were stably transfected with cloned fragments of mdv dna resulting in the expression of the mdv-specific phosphoprotein pp38. effector cells were obtained from p2a (b19b19) and s13 (b13b13) chickens at 7 days post inoculation with rev, oncogenic or attenuated serotype 1 mdv (jm-16/o and jm- ... | 1994 | 7725630 |
| identification and localization of glycoprotein b expression in lymphoid tissues of chickens infected with turkey herpesvirus. | one-day-old chickens were inoculated with turkey herpesvirus (hvt). using an indirect immunofluorescence assay with a monoclonal antibody against hvt glycoprotein b (gb), we determined the course of productive hvt infection in peripheral blood mononuclear cells (pbmcs), spleen, thymus, and bursa. pbmcs were examined from days 4 through 35 postinfection (pi). the spleen, thymus, and bursa were examined from 21 through 70 days pi. although productive infection in pbmcs was detected at 4 to 12 days ... | 1994 | 7832696 |
| characterization of proteins encoded by the short unique region of herpesvirus of turkeys by in vitro expression. | nine open reading frames mapping in the short unique (us) region of the genome of herpesvirus of turkeys (hvt) were expressed by in vitro transcription and translation. the observed m(r)s of us10, sorf3 and us2 were as predicted from the sequence but there were discrepancies between the observed and predicted m(r)s of us1, protein kinase, gi, gd and ge. these could be accounted for in most cases by post-translational and co-translational processing. analysis of the synthesized products at differ ... | 1994 | 7931161 |
| expression of a novel marek's disease virus type 1 (mdv1)-specific protein p40 in insect cells by a baculovirus vector and in chick embryo fibroblasts infected with mdv1. | we found that the open reading frame of 1053 nucleotides within the short unique sequence of marek's disease virus (mdv) type 1 (mdv1) expresses a 40 kda protein, using a baculovirus vector in insect cells. mouse antisera against the 40 kda protein reacted with a 40 kda protein in mdv1-infected cells. a cross-reactive polypeptide of 37 kda was also detected in cells infected with herpesvirus of turkey. | 1994 | 7979993 |
| integration of multiple chicken retroviruses into multiple chicken herpesviruses: herpesviral gd as a common target of integration. | integration of two different avian retroviruses, reticuloendotheliosis virus (rev) and avian leukosis virus (alv), into the genome of two different avian herpesviruses, the herpesvirus of turkeys (hvt) and marek's disease virus (mdv), was investigated. integration events occurred by the fourth and sixth in vitro passage of cells coinfected with rev/hvt and alv/mdv, respectively. in order to further characterize the integration events, integrated proviruses and surrounding herpesviral genetic mat ... | 1994 | 8030268 |
| gene organization in herpesvirus of turkeys: identification of a novel open reading frame in the long unique region and a truncated homologue of pp38 in the internal repeat. | the dna sequence of a 4.792-kb fragment comprising 3.176 kb of the long unique region (ul) and 1.605 kb of the internal repeat (irl) flanking ul of herpesvirus of turkeys (hvt) was determined. three potential open reading frames (orfs) and an origin of replication have been identified. orf 1, which maps entirely within ul, has the capacity to code for an 82k protein, 731 amino acids long, which has a counterpart in marek's disease virus (mdv) but not in other herpesviruses. orf 2 has the potenti ... | 1995 | 7871728 |
| virus-neutralization domains on the oligomeric (230 kda) forms of antigen b of herpesvirus of turkeys and marek's disease virus differ in cross-serotypic activity. | herpesvirus of turkeys (hvt) is frequently used to protect chickens against marek's disease (md). the hvt and mdv native antigen b complex shares common epitopes. to determine whether these oligomers present virus-neutralizing domains, monospecific antibodies to the hvt and mdv native 230 kda oligomers were produced. the monospecific antibody immunopurified from an anti-hvt avian serum neutralized the in vitro infectivity of the oncogenic isolate mdv-b and the vaccine strains cvi988, sb1 and hvt ... | 1995 | 7483903 |
| marek's disease and new approaches to its control. | marek's disease (md) is a lymphoproliferative disorder induced by a herpesvirus. several factors, including those virus-encoded and host-dependent, affect the course of the disease. existing vaccination program is based on the use of attenuated strains of md virus (mdv) serotype 1 and on strains of non-oncogenic serotype 2 (mdv2) and serotype 3 (herpesvirus of turkey-hvt) viruses. failures resulting in disease progress have been reported and indicate need for production of new, more effective va ... | 1995 | 7572471 |
| structure and properties of a herpesvirus of turkeys recombinant in which us1, us10 and sorf3 genes have been replaced by a lacz expression cassette. | in the process of generating an insertional mutant of herpesvirus of turkeys (hvt) expressing lacz at the protein kinase (pk) locus, we isolated a recombinant which contained an intact pk gene but the short unique regions us1, us10 and sorf3 had been deleted and replaced by the lacz cassette. moreover, the virus contained duplicate copies of gd, gi and ge in an opposite orientation flanking lacz, us2 and pk which were contiguous. these results are of interest in relation to the flexibility of th ... | 1995 | 7595402 |
| sequence characteristics of a gene in infectious laryngotracheitis virus homologous to glycoprotein d of herpes simplex virus. | an infectious laryngotracheitis virus (iltv, gallid herpesvirus 1) gene homologous to glycoprotein d of herpes simplex virus (hsv) was identified and characterized by its nucleotide and derived amino acid sequence. the iltv gd gene is located in the unique short region (u(s)) and contains an open reading frame capable of specifying a polypeptide of 380 amino acids, including n- and c- terminal hydrophobic domains consistent with signal and anchor regions respectively, and no potential sites for ... | 1995 | 7612933 |
| herpesvirus of turkey recombinant viruses expressing infectious bursal disease virus (ibdv) vp2 immunogen induce protection against an ibdv virulent challenge in chickens. | two recombinant herpesviruses of turkey (hvt) expressing the vp2 protein of infectious bursal disease virus (ibdv or gumboro disease virus) have been constructed: vhvt001 and vhvt002. the vp2 open reading frame was inserted at the locus of the small subunit of ribonucleotide reductase gene (hsv-1 ul40 homolog) without any exogenous promoter in vhvt001 and at the locus of gl gene (hsv-1 us7 homolog) under the control of the human cytomegalovirus immediate-early promoter in vhvt002. the isolation ... | 1995 | 7645252 |
| field safety and efficacy of in ovo administration of hvt + sb-1 bivalent marek's disease vaccine in commercial broilers. | the field safety and efficacy of a bivalent marek's disease (md) vaccine (herpesvirus of turkeys + strain sb-1) administered in ovo were evaluated. the vaccine was administered to commercial broiler chicken eggs using embrex's inovoject automated egg-injection system. comparisons were made with a conventional method of vaccination, in which the vaccine was administered subcutaneously to chicks at hatching. in ovo administration of the vaccine at day 17.5-18.5 of incubation significantly reduced ... | 1995 | 7677641 |
| characteristics of cvi988/rispens and r2/23, two prototype vaccine strains of serotype 1 marek's disease virus. | studies were focused on two attenuated serotype 1 marek's disease (md) vaccine viruses, cvi988/rispens (passage 42) and r2/23 (passage 105). both serotype 1 vaccine viruses provided much higher levels of protection than the prototype md vaccine, turkey herpesvirus (hvt); the best protection was generally provided by cvi988/rispens when compared with other vaccines. the efficacy of neither serotype 1 vaccine was improved by mixture with viruses of other serotypes (synergism). no differences betwe ... | 1995 | 7677647 |
| molecular evolution of infectious laryngotracheitis virus (iltv; gallid herpesvirus 1): an ancient example of the alphaherpesviridae? | an analysis of two essential genes of infectious laryngotracheitis virus (iltv), glycoprotein d (gd) and the immediate early gene, herpes simplex virus homologue icp27, was performed with the equivalent gene homologues from several alphaherpesviruses. amino acid (aa) sequence analysis revealed that these iltv genes shared limited homology to other alphaherpesvirus equivalents and were distinct from the two other avian herpesviruses, marek's disease virus (mdv) and herpesvirus of turkeys (hvt). s ... | 1995 | 8545960 |
| effect of serotype 2 and 3 marek's disease vaccines on the development of avian leukosis virus-induced pre-neoplastic bursal follicles. | the effect of serotype 2 and 3 marek's disease virus (mdv) vaccines on the development of pre-neoplastic bursal lesions induced by two strains of subgroup a avian leukosis virus (alv) was studied. chickens of line 15i5 x 7(1) susceptible to alv-induced lymphoma were inoculated at hatch with rous-associated virus-1 (rav-1) or strain rpl-40 of subgroup a alv. all chickens, except for negative controls, were also inoculated at hatch with strain 301b/1 of serotype 2 mdv or strain fc126 of turkey her ... | 1995 | 8719208 |
| protective efficacy of a recombinant herpesvirus of turkeys as an in ovo vaccine against newcastle and marek's diseases in specific-pathogen-free chickens. | we investigated the potential of a herpesvirus of turkey (hvt)-based recombinant virus (rhvt) as an in ovo vaccine to protect specific-pathogen-free chickens against newcastle disease (nd) and marek's disease (md). the rhvt, designed to express fusion (f) and hemagglutinin-neuraminidase (hn) glycoproteins of the lentogenic hitchner b1 strain of nd virus (ndv), as well as glycoproteins a and b of the ga strain of serotype 1 md virus (mdv) was efficacious in protecting chickens against nd and md. ... | 1996 | 8782342 |
| protection and synergism by recombinant fowl pox vaccines expressing genes from marek's disease virus. | recombinant fowl poxviruses (rfpv) were constructed to express genes from serotype 1 marek's disease virus (mdv) coding for glycoproteins b (gb1), c (gc), and d (gd) and tegument proteins ul47 and ul48, as well as genes from serotypes 2 and 3 mdv coding for glycoprotein b (gb2 and gb3). these rfpvs, alone and in various combinations, including combinations of fowl poxvirus (fpv)/gbs with turkey herpesvirus (hvt), were evaluated for ability to protect maternal antibody-positive (ab+) and -negativ ... | 1996 | 8790888 |
| genetic susceptibility to marek's disease virus of local chickens in taiwan. | marek's disease is a contagious disease in chickens caused by marek's disease virus (mdv) infection. invasion of very virulent mdv (vvmdv) was considered to be a major cause of vaccine break, resulting in a large economic loss in the poultry industry. two strains of the vvmdv (strains ltb-1 and lts-1) have been reported in taiwan, causing early mortality and formation of lymphoid tumors in broilers and layers. in this study, we report the susceptibility of local chickens in taiwan to inoculation ... | 1996 | 8883787 |
| influence of chicken genotype on protection against marek's disease by a herpesvirus of turkeys recombinant expressing the glycoprotein b (gb) of marek's disease virus. | two inbred lines of white leghorn chickens which differ in b-haplotype were immunized at 2 days of age with a thymidine kinase negative (tk-ve) herpesvirus of turkeys (hvt) recombinant expressing the glycoprotein b (gb) gene of marek's disease virus (mdv) and were challenged 6 days later with 1000 p.f.u. of the highly virulent rb1b strain of mdv. mock-vaccinated chickens and chickens immunized with a spontaneous tk-ve hvt mutant served as controls. genetically resistant b21 chickens were protect ... | 1996 | 8920698 |
| identification of a potential marek's disease virus serotype 2 glycoprotein d gene with homology to herpes simplex virus glycoprotein d. | the gene of marek's disease virus (mdv) serotype 2 (mdv2) homologous to glycoprotein d (gd) of herpes simplex virus (hsv) was identified and characterized by its nucleotide and predicted amino acid sequences. the mdv2 gd homologous gene contains an open reading frame capable of specifying a polypeptide of 385 amino acids, which include n- and c-terminal hydrophobic domains consistent with signal and anchor regions, respectively, and two potential n-linked glycosylation sites, one of which was lo ... | 1996 | 8973534 |
| onset of protective immunity in chicks after vaccination with a recombinant herpesvirus of turkeys vaccine expressing newcastle disease virus fusion and hemagglutinin-neuraminidase antigens. | the onset of protective immunity from lethal newcastle disease virus (ndv) challenge of chicks was determined after vaccination with a recombinant herpes virus of turkeys (hvt) expressing the fusion and hemagglutinin-neuraminidase proteins of ndv. one-day-old specific-pathogen-free chicks devoid of maternal antibodies to ndv were vaccinated with 130 to 3300 plaque forming units of hvt (depending on the trial) and then challenged at 4, 7, 10, and 14 days postvaccination (dpv) with a neurotropic v ... | 1996 | 8980804 |
| antigen-specific lymphoproliferative responses to tetanus toxoid: a means for the evaluation of marek's disease virus-induced immunosuppression in chickens. | antigen-specific lymphoproliferative responses were examined in chickens following immunization with tetanus toxoid (ttx). the immune competence of chickens was assessed by mitogen assay utilizing phytohemagglutinin (pha)-stimulation and ttx-specific antigen proliferation assay (ttx-apa). immune spleen cells but not peripheral blood leucocytes demonstrated specific proliferation following stimulation in vitro in a ttx-apa. in this study, we examined firstly the effects of marek's disease (md)-as ... | 1996 | 9032901 |
| a comparative study of histological conditions suitable for both immunofluorescence and in situ hybridization in the detection of herpesvirus and its antigens in chicken tissues. | our objective was to identify an optimal single set of conditions for use in both indirect immunofluorescence assays (ifa) and in situ hybridization (ish) to detect viral proteins and nucleic acids in avian lymphoid and neural tissues. various fixatives were evaluated for use with ifa to detect turkey herpesvirus (hvt) glycoprotein b (gb) and ish to identify hvt mrna in chicken tissues. a precipitating fixative (acetone) was compared to crosslinking fixatives [buffered glutaraldehyde-picric acid ... | 1996 | 8648086 |
| enhancement of reticuloendotheliosis virus-induced bursal lymphomas by serotype 2 marek's disease virus. | the effect of serotype 2 and 3 marek's disease virus (mdv) vaccines on the pathogenesis of reticuloendotheliosis virus (rev)-induced bursal and non-bursal lymphomas was examined in chickens of rprl lines 15i(5) x 7(1) and 6(3) x 0, respectively. at hatch, chickens were vaccinated with strain 301b/1 of serotype 2 mdv or strain fc126 of turkey herpesvirus (hvt), a serotype 3 mdv and inoculated with spleen necrosis virus (snv), a non-defective strain of rev. in another experiment, bursas from 14-we ... | 1996 | 18645839 |
| comparison of the genomes of 15 avian herpes-virus isolates by restriction endonuclease analysis. | differentiation of herpesvirus isolates has been performed mostly on the basis of biological properties and serology. in this study, 15 herpesvirus isolates from different species of birds were compared by restriction endonuclease analysis of genomic dna. all herpesviruses were isolated in europe or are used as vaccine viruses there. the isolates could be differentiated into seven groups based on restriction patterns. the largest group contains isolates from passeriform and psittacine birds and ... | 1997 | 18483909 |
| increased virulence of marek's disease virus field isolates. | the continuation of an apparent evolutionary trend of marek's disease virus (mdv) towards greater virulence may explain recent increased losses from marek's disease (md) in vaccinated flocks. to address this question, the virulence of 31 isolates of serotype 1 mdv obtained from layer or broiler flocks between 1987 and 1995 were characterized. each isolate was cultured in duck embryo fibroblasts for four to six passages, and ascertained to be free from contamination with avian retroviruses, chick ... | 1997 | 9087332 |
| efficacy of inovoject egg injection system for delivering marek's disease vaccine under hatchery conditions. | the ability of the inovoject egg injection system to effectively deliver the appropriate titer of herpesvirus of turkeys (hvt) vaccine during normal hatchery operation was evaluated. the inovoject machines configured for the jamesway and chickmaster commercial hatchers both maintained the integrity of the hvt vaccine without an appreciable loss in titer from the diluted vaccine to the egg. | 1997 | 9201414 |
| identification and dna sequence analysis of the marek's disease virus serotype 2 genes homologous to the thymidine kinase and ul24 genes of herpes simplex virus type 1. | the thymidine kinase (tk) gene has been used as a safe and convenient locus for expression of heterologous proteins in some alphaherpesviruses including herpesvirus of turkeys (hvt) antigenically related to marek's disease virus (mdv) serotypes 1 (mdv1) and 2 (mdv2). in mdv2 strain hprs 24 genome, genes equivalent to the tk and ul24 homologues of herpes simplex virus type 1 were identified and sequenced. the mdv2 ul24 gene overlaps the 5' end of the tk gene in a head-to-head orientation. the pre ... | 1997 | 9208458 |
| recombinant fowlpox virus vaccines against australian virulent marek's disease virus: gene sequence analysis and comparison of vaccine efficacy in specific pathogen free and production chickens. | we have cloned and sequenced the glycoprotein genes gb, gc and gd of the australian virulent marek's disease virus (mdv) isolate woodlands no. 1. the glycoprotein gb and gc sequences were identical to the homologs of other virulent mdv type 1 strains, and the glycoprotein gd sequence contained only one unique amino acid substitution. recombinant fowlpox viruses (rfpvs) expressing the mdv glycoprotein genes were constructed and their efficacy as vaccines was evaluated in specific pathogen free (s ... | 1997 | 9255932 |
| herpesvirus of turkeys homologue of hsv vp16 is structurally related to varicella zoster virus trans-inducing protein encoded by orf 10. | expression of the immediate-early genes of alpha-herpesviruses is stimulated by a family of trans-inducing factors represented by vp16 of hsv-1 and orf10 gene product of vzv. we have identified and determined the nucleotide sequence of the ul48 gene encoding the herpesvirus of turkeys (hvt) homologue of hsv vp16. the gene maps to the bamhi-j fragment and appears to be expressed in a form of bicistronic transcript together with ul49. the deduced amino acid sequence of the protein encoded by hvt u ... | 1997 | 9354269 |
| a simple immunoperoxidase plaque assay to detect and quantitate marek's disease virus plaques. | we report an immunoperoxidase-based staining technique that can be used to rapidly and accurately detect and quantitate marek's disease virus (mdv) plaques. monolayer cultures were fixed and incubated with a monoclonal antibody specific for mdv. after washing, a second antibody of horseradish peroxidase-conjugated goat anti-mouse igg was applied, incubated for 1 hr, and washed with phosphate-buffered saline. after the cultures were incubated with diaminobenzidine, cocl2, and h2o2, the plaques ap ... | 1997 | 9356696 |
| serological characteristics of a membrane glycoprotein gp82 of marek's disease virus. | marek's disease virus (mdv) is a cell-associated herpesvirus of gallinaceous fowl. glycoproteins of herpesviruses have been reported to be important in stimulating humoral and cell-mediated immune responses against viral infection. at least eight glycoproteins of mdv have been identified, and glycoprotein b (gb) has been reported to produce protective immunity. in addition to glycoproteins, other cell membrane proteins may also be important for engendering immunity. in an attempt to determine wh ... | 1997 | 9454915 |
| pathogenesis of marek's disease (md) and possible mechanisms of immunity induced by md vaccine. | marek's disease (md) is a lymphoproliferative disease of chicken, which is characterized by malignant t cell-lymphoma formation. this disease can be effectively prevented by vaccination with attenuated md virus (mdv), apathogenic mdv or herpesvirus of turkey. md vaccines are ones of a few vaccines which can prevent virus-induced tumor among mammalian and avian species. to determine the roles of t cell subsets in the protection mechanism, chickens vaccinated with an attenuated mdv (cvi988) were d ... | 1998 | 9492353 |
| latent turkey herpesvirus infection in lymphoid, nervous, and feather tissues of chickens. | in earlier studies, we found that a late gene product, glycoprotein b (gb) was highly expressed in lymphoid tissues of chickens inoculated with turkey herpesvirus (hvt). the objectives of the present study were twofold. first, we wanted to expand on our previous research and determine if gb expression declines or disappears during later time periods of hvt infection. second, we wanted to correlate gb expression with presence of hvt, i.e. if gb expression is absent, can hvt still be detected? fif ... | 1998 | 9645320 |
| the genetic organization and transcriptional analysis of the short unique region in the genome of nononcogenic marek's disease virus serotype 2. | studies on the marek's disease virus (mdv) serotype 2 (mdv2) genome may be important for understanding the naturally nononcogenic nature of the virus. to determine the complete dna sequence of mdv2 unique short (us) region, genomic bamhi fragments f, m1 and r were sequenced. the mdv2 us region is 12109 bp long and contains 12 potential open reading frames (orfs) likely to encode for proteins. seven of them exhibit homologies to herpes simplex virus type 1 (hsv-1) us1 (icp22), us2, us3 (protein k ... | 1998 | 9879770 |
| response of embryonic chicken lymphocytes to in ovo exposure to lymphotropic viruses. | to examine effects of virus exposure on embryonic lymphoid organ structure, apoptosis, and lymphoid cell subpopulations. | 1999 | 10451200 |
| atherosclerosis induced by infection with marek's disease herpesvirus in chickens. | this research was suggested after crystals that we observed in herpesvirus-infected cell cultures were identified as cholesterol. other reports and the development of defined reagents led us to select the use of marek's disease herpesvirus (mdv) infection of chickens to demonstrate a potential role of herpesviruses in the pathogenesis of atherosclerosis. available for our use were a clone-purified strain of mdv of known virulence, genetically selected, specific pathogen-free chickens, and approp ... | 1999 | 10539849 |
| an acute form of transient paralysis induced by highly virulent strains of marek's disease virus. | a novel syndrome was observed after inoculation of 3-wk-old chickens with highly virulent marek's disease virus (mdv) strains. this syndrome was characterized by the acute onset of neurologic signs including flaccid paralysis of neck and limbs 9-10 days postinoculation, typically resulting in death 1-3 days after the onset of clinical signs. most affected birds died, and spontaneous recovery was rare. few if any gross tissue changes were found. histologic brain lesions included acute vasculitis ... | 1999 | 10611987 |
| effect of native chicken interferon on mdv replication. | marek's disease virus (mdv) is an oncogenic alphaherpesvirus. its specific phosphorylated protein, pp38 has been implicated in mdv oncogenesis. in order to check whether the known anti-viral or anti-proliferative actions of interferon (ifn) are of importance in marek's disease (md), chicken embryo fibroblasts (cefs) were infected with attenuated serotype-1 mdv strain cvi988, or with herpesvirus of turkeys (hvt). different concentrations of native chicken ifn were added to the cell cultures, prio ... | 1999 | 10696431 |
| evidence for marek's disease in turkeys in germany: detection of mdv-1 using the polymerase chain reaction. | since 1994 tumorous lesions have been monitored in turkeys on three farms in germany. on one of these farms, chickens also had tumorous lesions. affected turkeys were retarded in growth, apathic, pale and almost unable to move. the older the animals got, the more indistinct the clinical signs became. mortality started at an age of 5 weeks and reached between 20% and 60% by the end of the fattening period of about 20 weeks. the aetiological differential diagnosis includes reticuloendotheliosis (r ... | 1999 | 10696435 |
| tissue-specific restriction of latent turkey hvt transcription. | using in situ hybridization, latent turkey herpesvirus (hvt) transcription was examined in lymphoid and/or nonlymphoid tissues. blood samples were taken for virus isolation from chickens at 7 and 240 days post infection (pi) representing time points for productive and latent turkey herpesvirus infections, respectively. spleen, thymus, sciatic and brachial nerves from infected chickens were analyzed for latent hvt transcription and hvt glycoprotein b (gb) expression at 240 days pi. using indirect ... | 1999 | 10696436 |
| non-essential loci in the bamhi-i and -f fragments of the hvt fc126 genome. | the sequence of bamhi-i fragment of the herpesvirus of turkeys (hvt) fc126 strain dna was analyzed for the presence of potential open reading frames (orfs). four complete (orfs 2 to 5) and 2 partial orfs (orfs 1 and 6) were detected. orfs 2 and 3 were homologous to the hsv-1 ul55 and the ehv-1 gene 3, respectively. the orf 6 was already partially sequenced by smith et al. (virology 207, 205-216, 1995), and was homologous to a marek's disease virus (mdv) orf located in a similar position (orf 21; ... | 1999 | 10696442 |
| vaccination of broilers with hvt expressing an eimeria acervulina antigen improves performance after challenge with eimeria. | the sequence encoding part of the 100 kda refractile body protein (ea1a) from eimeria acervulina was cloned into the us10 locus of herpesvirus of turkeys (hvt) downstream of ltr promoter. expression of the fusion protein was shown in vitro. recombinant hvt showed a delayed and slightly reduced level of viremia compared to the parent strain in spf chickens as well as in broilers. effect on the performance of broilers vaccinated with recombinant or parent hvt was measured by challenge at day 24 wi ... | 1999 | 10696444 |
| efficacy of a combination vaccine containing mdv cvi 988 strain and hvt against challenge with very virulent mdv. | with the emergence of very virulent marek's disease virus (mdv) strains, vaccines based on herpesvirus of turkeys (hvt) appear to be not powerful enough to confer full protection, whereas in chicken flocks vaccinated with mdv cvi 988 strain protective immunity sometimes is generated not early enough for full protection. for this reason combination vaccines containing hvt as well as cvi 988 have been developed. in this paper the beneficial effect of combining both types of virus strains in one va ... | 1999 | 10696445 |
| a recombinant fowlpox virus vaccine expressing glycoprotein b gene from cvi988/rispens strain of mdv: protection studies in different chickens. | recombinant fowlpox virus (rfpv) was constructed to express glycoprotein b (gb) gene from cvi988/rispens strain of marek's disease virus (mdv). the rfpv-gb/r alone and in combination with herpesvirus of turkey (hvt) preparations were evaluated for their protective efficacy against challenge with very virulent mdv strains md5 and rb1b in different chickens. the rfpv-gb/r alone induced protection comparable to that by hvt vaccines in both ab- spf chickens and ab+ production chickens. significant p ... | 1999 | 10696446 |
| enhancement of spontaneous bursal lymphoma frequency by serotype 2 marek's disease vaccine, sb-1, in transgenic and non-transgenic line 0 white leghorn chickens. | a significant incidence of bursal lymphomas with long latencies was noted in transgenic breeders carrying a benign defective subgroup a avian leukosis provirus, alva6, in their germline and maintained free of exposure to avian retroviruses. serotype 2 marek's disease (md) vaccine virus, strain sb-1, a component of the bivalent md vaccine used to vaccinate the breeders, was suspected as a contributory factor in the increased bursal lymphoma incidence. although these bursal lymphomas had several c ... | 1999 | 26911501 |
| inhibitory effects of nitric oxide and gamma interferon on in vitro and in vivo replication of marek's disease virus. | the replication of marek's disease herpesvirus (mdv) and herpesvirus of turkeys (hvt) in chicken embryo fibroblast (cef) cultures was inhibited by the addition of s-nitroso-n-acetylpenicillamine, a nitric oxide (no)-generating compound, in a dose-dependent manner. treatment of cef culture, prepared from 11-day-old embryos, with recombinant chicken gamma interferon (rchifn-gamma) and lipopolysaccharide (lps) resulted in production of no which was suppressed by the addition of n(g)-monomethyl l-ar ... | 2000 | 10729136 |
| nucleotide sequence of the gene encoding the major capsid protein of herpesvirus of turkeys. | the gene encoding the major capsid protein (mcp) vp5 of herpesvirus of turkeys (hvt) was identified and sequenced. it has a single open reading frame of 4236 nucleotides encoding 1412 aa protein. the gene is flanked by vp23 and ul20 sequences and is localized in the unique long region (ul) within the bamhi-b fragment. comparison of amino acid homology has shown its clear position among the alpha-herpesviruses rather than beta- or gamma-herpesviruses. the vp5 is expressed from polycistronic mrna ... | 2000 | 10872871 |
| the genome of a very virulent marek's disease virus. | here we present the first complete genomic sequence, with analysis, of a very virulent strain of marek's disease virus serotype 1 (mdv1), md5. the genome is 177,874 bp and is predicted to encode 103 proteins. mdv1 is colinear with the prototypic alphaherpesvirus herpes simplex virus type 1 (hsv-1) within the unique long (ul) region, and it is most similar at the amino acid level to mdv2, herpesvirus of turkeys (hvt), and nonavian herpesviruses equine herpesviruses 1 and 4. mdv1 encodes 55 hsv-1 ... | 2000 | 10933706 |
| transactivation of latent marek's disease herpesvirus genes in qt35, a quail fibroblast cell line, by herpesvirus of turkeys. | the qt35 cell line was established from a methylcholanthrene-induced tumor in japanese quail (coturnix coturnix japonica) (c. moscovici, m. g. moscovici, h. jimenez, m. m. lai, m. j. hayman, and p. k. vogt, cell 11:95-103, 1977). two independently maintained sublines of qt35 were found to be positive for marek's disease virus (mdv)-like genes by southern blotting and pcr assays. sequence analysis of fragments of the icp4, icp22, icp27, vp16, meq, pp14, pp38, open reading frame (orf) l1, and glyc ... | 2000 | 11024146 |
| the genome of turkey herpesvirus. | here we present the first complete genomic sequence of marek's disease virus serotype 3 (mdv3), also known as turkey herpesvirus (hvt). the 159,160-bp genome encodes an estimated 99 putative proteins and resembles alphaherpesviruses in genomic organization and gene content. hvt is very similar to mdv1 and mdv2 within the unique long (ul) and unique short (us) genomic regions, where homologous genes share a high degree of colinearity and their proteins share a high level of amino acid identity. w ... | 2001 | 11134310 |
| adaptation of marek's disease virus to the vero continuous cell line. | marek's disease virus (mdv) is a highly infectious, cell-associated oncogenic herpesvirus. production of md vaccines has been limited to primary chicken and duck embryo fibroblast (cef and def) cultures. these have a limited life span and cannot be readily stored in liquid nitrogen. moreover, the need to prepare cef and def cells on a regular basis from 10 to 11 day-old embryos derived from a flock that must be tested continuously for the presence of avian pathogens adds to the cost of vaccine p ... | 2001 | 11230930 |
| herpesvirus connection in the expression of autoimmune vitiligo in smyth line chickens. | the smyth line (sl) chicken is an animal model for human vitiligo, a common acquired depigmentary disorder affecting about 1-2% of people worldwide. the vitiligo-like depigmentation in sl chickens typically develops when the birds are between 6 and 14 weeks of age and may affect 70-95% of hatch mates. the development of sl vitiligo is considered to depend on two interacting components, namely an inherent melanocyte defect and an autoimmune reaction to melanocytes. recently, a role for an environ ... | 2001 | 11277493 |
| the genome of herpesvirus of turkeys: comparative analysis with marek's disease viruses. | the complete coding sequence of the herpesvirus of turkeys (hvt) unique long (u(l)) region along with the internal repeat regions has been determined. this allows completion of the hvt nucleotide sequence by linkage to the sequence of the unique short (u(s)) region. the genome is approximately 160 kbp and shows extensive similarity in organization to the genomes of marek's disease virus serotypes 1 and 2 (mdv-1, mdv-2) and other alphaherpesviruses. the hvt genome contains 75 orfs, with three orf ... | 2001 | 11297687 |
| no effect of turkey herpesvirus infections on the outcome of avian pneumovirus infections in turkeys. | to determine whether turkey herpesvirus (hvt) impairs the aspecific and specific defense against an avian pneumovirus (apv) infection, specific-pathogen-free turkeys were inoculated at 7 days of age with hvt and 1, 5, or 7 wk later with apv. clinical signs, apv replication, and development of antibodies against apv were evaluated. no differences were found between the birds that received both hvt and apv and those that received only apv. | 2001 | 11417838 |
| early posthatch protection against marek's disease in chickens vaccinated in ovo with a cvi988 serotype 1 vaccine. | cvi988, a serotype 1 marek's disease virus (mdv), was used as an in ovo vaccine in specific-pathogen-free chickens to determine if this virus induces early posthatch protection against marek's disease as has been shown previously for turkey herpesvirus. mdv cvi988 was injected at embryonation day (ed) 17 (group 1) or at hatch (group 2). a third group (group 3) was left unvaccinated. at 1, 2, 3, 4, 5, and 7 days of age, chickens from each group were sampled and examined as follows: a) single-cell ... | 2001 | 11569737 |
| influence of vaccine deposition site on post-vaccinal viraemia and vaccine efficacy in broiler chickens following in ovo vaccination against marek's disease. | in ovo vaccination against marek's disease is a widely used technology in the broiler industry.a series of experiments was carried out to determine the site of vaccine deposition in the egg during automated in ovo vaccination, and the effect of vaccine deposition site and dose on vaccine responses following vaccination with cell-associated herpesvirus of turkeys in commercial broiler chickens. vaccine deposition site following automated in ovo vaccination was principally influenced by the age of ... | 2001 | 19184942 |
| innovation and discovery: the application of nucleic acid-based technology to avian virus detection and characterization. | polymerase chain reaction (pcr)-based approaches to the detection, differentiation and characterization of avian pathogens continue to be developed and refined. the pcrs, or reverse transcriptase-pcrs, may be general, designed to detect all or most variants of a pathogen, or to be serotype, genotype or pathotype specific. progress is being made with respect to making nucleic acid approaches more suitable for use in diagnostic laboratories. robotic workstations are now available for extraction of ... | 2001 | 19184952 |
| marek's disease in broiler chickens: effect of route of infection and herpesvirus of turkey-vaccination status on detection of virus from blood or spleen by polymerase chain reaction, and on weights of birds, bursa and spleen. | the polymerase chain reaction (pcr) has recently emerged as an additional tool for the monitoring and diagnosis of marek's disease. we investigated a number of factors that may influence the interpretation of pcr results in commercial broiler chickens including the effects of route of infection and herpesvirus of turkeys (hvt)-vaccination status. we also investigated the suitability of peripheral blood lymphocytes (pbl) and spleen as tissues for marek's disease virus (mdv) detection. hvt-vaccina ... | 2001 | 19184956 |
| complete, long-lasting protection against lethal infectious bursal disease virus challenge by a single vaccination with an avian herpesvirus vector expressing vp2 antigens. | marek's disease herpesvirus is a vaccine vector of great promise for chickens; however, complete protection against foreign infectious diseases has not been achieved. in this study, two herpesvirus of turkey recombinants (rhvts) expressing large amounts of infectious bursal disease virus (ibdv) vp2 antigen under the control of a human cytomegalovirus (cmv) promoter or cmv/beta-actin chimera promoter (pec promoter) (rhvt-cmvvp2 and rhvt-pecvp2) were constructed. rhvt-pecvp2, which expressed the v ... | 2002 | 11991992 |
| evaluation of an early granulocytic response of chick embryos inoculated with herpesvirus of turkeys. | 1. early granulocytic response was evaluated in chick embryos inoculated with herpesvirus of turkeys (hvt). 2. fifty 10-d-old specific pathogen-free embryos were divided into two groups, inoculated via yolk sac. group 1 were inoculated with a complete dose of hvt and group 2 with vaccinediluent only. 3. samples were taken for histological evaluation of yolk sac, liver, chorioallantoic membrane, brain and heart from 5 embryos per group on days 12, 14, 16, 18 and 20 of embryonic life. 4. increases ... | 2002 | 12047084 |
| effect of in ovo vaccine delivery route on herpesvirus of turkeys/sb-1 efficacy and viremia. | a study was designed to ascertain the influence of in ovo site of inoculation and embryonic fluid type on the development of marek's disease (md) vaccine viremia and efficacy against md challenge. the experiments were divided into in vitro and in vivo phases. in the in vitro phase, herpesvirus of turkeys/sb-1 vaccine was combined with basal medium eagle (bme) medium (control), amniotic fluid, or allantoic fluid and subsequently titrated on secondary chick embryo fibroblast cultures. there were n ... | 2002 | 12061635 |
| marek's disease in turkeys. i. a seven-year survey of commercial flocks and experimental infection using two field isolates. | marek's disease virus (mdv) causes immunosuppression and tumors in chickens, but the turkey is an unusual host for the virus, and tumors caused by mdv in turkeys are unique. we describe the prevalence of turkey tumors in israel between 1993 and 2000, their molecular diagnosis by polymerase chain reaction (pcr), and the natural distribution of herpesvirus of turkeys (hvt). most clinical cases with tumors in commercial turkeys were diagnosed as mdv. the reproduction of marek's disease (md) in turk ... | 2002 | 12061640 |
| recent increase of marek's disease in korea related to the virulence increase of the virus. | the incidence of marek's disease (md), an important neoplastic disease of chickens, suddenly increased in 1997 in korea. most md cases of this country were detected in chickens over 20 wk of age. five md viruses were isolated from field flocks in which severe md losses had occurred, and one of the viruses was studied to compare its pathotype with the prototype jm strain. the isolate komd-ic induced severe depression not only in body weight but also in relative bursal weight, and the depression b ... | 2002 | 12243513 |
| field trial in commercial broilers with a multivalent in ovo vaccine comprising a mixture of live viral vaccines against marek's disease, infectious bursal disease, newcastle disease, and fowl pox. | a multivalent in ovo vaccine (miv) was tested for safety and efficacy in a commercial broiler complex. the miv comprised five replicating live viruses including serotypes 1, 2, and 3 of marek's disease virus (mdv), an intermediate infectious bursal disease virus (ibdv) and a recombinant fowl poxvirus (fpv) vector vaccine containing hn and f genes of newcastle disease virus (ndv). the performance of miv-vaccinated broilers was compared with that of hatchmates that received turkey herpesvirus (hvt ... | 2002 | 12243525 |
| immunosuppressive effects of marek's disease virus (mdv) and herpesvirus of turkeys (hvt) in broiler chickens and the protective effect of hvt vaccination against mdv challenge. | much of the impact of marek's disease in broiler chickens is considered to be due to immunosuppression induced by marek's disease virus (mdv). the present study evaluates the effects of an australian isolate of pathogenic mdv (strain mpf 57) and a non-pathogenic vaccinal strain of herpesvirus of turkeys (hvt) (strain fc 126) on the immune system of commercial broiler chickens for 35 days following challenge at days 0 or 3 of age. it also investigates the extent of protection provided by hvt vacc ... | 2002 | 12427339 |
| antiviral and antitumoral effects of recombinant chicken myelomonocytic growth factor in virally induced lymphoma. | chicken myelomonocytic growth factor (cmgf) is a 27-kda glycoprotein that stimulates the growth and activation of cells from the monocyte/macrophage lineage. recombinant cmgf was produced in a prokaryotic (escherichia coli) expression system and purified via a c-terminal his-tag. treatment of 2-week-old histocompatible b(13)/b(13) chickens highly susceptible to marek's disease (md) with rcmgf (two daily injections of 50 microg rcmgf per chicken) enhanced background and lps-inducible systemic no ... | 2002 | 12433057 |
| immunological tolerance in chickens hatching from eggs injected with cell-associated herpesvirus of turkey (hvt). | cell-associated herpesvirus of turkeys (hvt) was inoculated in ovo at various stages of incubation. chickens hatching from these eggs were tested for anti-hvt antibodies by several serologic procedures including enzyme-linked immunosorbent assay, indirect immunofluorescence assays, and western blot. viremic chickens that remained free of detectable antibodies were considered tolerant to hvt. chickens exposed to hvt at embryonation day 14 or earlier had 6-33% incidence of tolerance. tolerant chic ... | 2003 | 12631525 |
| melanocyte-specific cell mediated immune response in vitiliginous smyth line chickens. | smyth line chickens (sl) are an animal model to study human autoimmune vitiligo. vitiligo in sl chickens is characterized by a post-hatch loss of feather melanocytes (mc) resulting in feather depigmentation. the etiopathology of this disease remains unclear, however, a hypothesis that cell-mediated immunity (cmi) plays an important role in the destruction of melanocytes has been proposed based on previous studies. to support this hypothesis, the delayed wattle response (dwr) to mc lysates was ex ... | 2003 | 12935784 |
| protection and synergism by recombinant fowl pox vaccines expressing multiple genes from marek's disease virus. | recombinant fowl poxviruses (rfpvs) were constructed to express genes from serotype 1 marek's disease virus (mdv) coding for glycoproteins b, e, i, h, and ul32 (gb1, ge, gi, gh, and ul32). an additional rfpv was constructed to contain four mdv genes (gb1, ge, gi, and ul32). these rfpvs were evaluated for their ability to protect maternal antibody-positive chickens against challenge with highly virulent mdv isolates. the protection induced by a single rfpv/gb1 (42%) confirmed our previous finding ... | 2003 | 14562881 |
| characterization of vnr-13, the first alphaherpesvirus gene of the bcl-2 family. | the bcl-2 family, including antiapoptotic and proapoptotic members, plays key regulating roles in programmed cell death. we report the characterization of a new member of the bcl-2 family, encoded by herpesvirus of turkeys (hvt). the product of this gene shares 80% homology with nr-13, an apoptosis inhibitor, which is overexpressed in avian cells transformed by the v-src oncogene. this new gene, that we propose to call vnr-13, is the first member of the bcl-2 family to be isolated among alpha-he ... | 2003 | 14644608 |
| marek's disease virus research in the post-sequencing era: new tools for the study of gene functions and virus-host interactions. | despite the fact that the causative agent of marek's disease was described more than 30 years ago, and that subsequently many classical biological studies have been carried out on the marek's disease virus (mdv), detailed analysis of its gene functions has been hampered by lack of suitable research tools. information on the primary structure of mdv-1 and its serologically related viruses, mdv-2 and herpesvirus of turkeys, is now available. this review focuses on the introduction of the modern an ... | 2003 | 17585455 |
| field evaluations of safety and efficacy of an australian marek's disease vaccine. | to demonstrate the safety and efficacy of the marek's disease virus-1 vaccine (strain bh 16) from field studies in comparison with the cvi 988 rispens vaccine currently available in australia. | 2003 | 15080446 |
| marek's disease vaccination, with turkey herpesvirus, and enrofloxacin modulate the activities of hepatic microsomal enzymes in broiler chickens. | chickens were vaccinated against marek's disease intramuscularly at one day of age. enrofloxacin was given ad libitum in the drinking water at concentrations of 50, 100 and 250 mg/l from 8 days to 13 days of age when the animals were killed and the activities of cytochrome p-450 enzymes in the liver were measured. vaccinated non-treated chickens served as a positive control. a negative control group was neither vaccinated nor treated. vaccination decreased the activity of aniline hydroxylase and ... | 2004 | 15168752 |