Publications

rnai-based functional genomics identifies new virulence determinants in mucormycosis.	mucorales are an emerging group of human pathogens that are responsible for the lethal disease mucormycosis. unfortunately, functional studies on the genetic factors behind the virulence of these organisms are hampered by their limited genetic tractability, since they are reluctant to classical genetic tools like transposable elements or gene mapping. here, we describe an rnai-based functional genomic platform that allows the identification of new virulence factors through a forward genetic appr ...	2017	28107502
effect of the filamentous fungus mucor circinelloides on the development of eggs of the rumen fluke calicophoron daubneyi (paramphistomidae).	ruminants infected by paramphistomidae flukes shed eggs in the feces, which pass through different stages in the environment until the infective stages (metacercariae) are reached. the activity of the soil fungus mucor circinelloides on the development of eggs of the rumen fluke calicophoron daubneyi was presently tested with 3 probes, i.e., in petri plates, feces and an aqueous environment (tubes). the effect of the fungus was assessed by recording the numbers of undeveloped, non-viable and emb ...	2017	28165868
a protein complex bearing an oxidase with napthalene dihydrodiol dehydrogenase activity is induced in mucor circinelloides strain yr-1 during growth on polycyclic aromatic compounds.	fungi are organisms capable of growing in a myriad of conditions and respond to counteract environmental cues. several locations in the world are polluted with oil and its derivatives, and some microorganisms tolerant to these compounds have been isolated. some fungi can grow in the presence of molecules such as polycyclic aromatic hydrocarbons as sole carbon sources. in this report, we further characterized the induced enzymes with phenanthrene from mucor circinelloides yr-1 strain, isolated fr ...	2018	28975438
entomophthoromycosis and mucormycosis as causes of pneumonia in vietnamese potbellied pigs.	two vietnamese potbellied pigs ( sus scrofa) had respiratory disease and, on autopsy, both pigs had large masses in the lungs and thoracic cavity. microscopically, pulmonary and pleural masses contained large areas with hyphae surrounded by hypereosinophilic cellular debris rimmed by abundant eosinophils, lymphocytes, plasma cells, and histiocytes with occasional multinucleate giant cells. the hypereosinophilic debris usually formed tight cuffs, or "sleeves" around the hyphae, compatible with sp ...	2018	29034814
mucor circinelloides induces platelet aggregation through integrin αiibβ3 and fcγriia.	thrombosis is a hallmark of the fatal fungal infection mucormycosis. yet, the platelet activation pathway in response to mucormycetes is unknown. in this study we determined the platelet aggregation potential of mucor circinelloides (m. circinelloides) nrrl3631, characterized the signaling pathway facilitating aggregation in response to fungal spores, and identified the influence of the spore developmental stage upon platelet aggregation potential. using impedance and light-transmission aggregom ...	2018	29297721
identification and characterization of diacylglycerol acyltransferase from oleaginous fungus mucor circinelloides.	acyl-coa:diacylglycerol acyltransferase (dgat) is a pivotal regulator of triacylglycerol (tag) synthesis. the oleaginous fungus mucor circinelloides has four putative dgats: mcdgat1a, mcdgat1b, mcdgat2a, and mcdgat2b, classified into the dgat1 and dgat2 subfamilies, respectively. to identify and characterize dgats in m. circinelloides, these four genes were expressed in saccharomyces cerevisiae h1246 (tag-deficient quadruple mutant), individually. tag biosynthesis was restored only by the expres ...	2018	29260551