Publications

construction of a recyclable genetic marker and serial gene deletions in the human pathogenic mucorales mucor circinelloides.	mucor circinelloides is a human pathogen, biofuel producer, and model system that belongs to a basal fungal lineage; however, the genetics of this fungus are limited. in contrast to ascomycetes and basidiomycetes, basal fungal lineages have been understudied. this may be caused by a lack of attention given to these fungi, as well as limited tools for genetic analysis. nonetheless, the importance of these fungi as pathogens and model systems has increased. m. circinelloides is one of a few geneti ...	2017	28476909
control of morphology and virulence by adp-ribosylation factors (arf) in mucor circinelloides.	mucor circinelloides is a dimorphic fungus used to study cell differentiation that has emerged as a model to characterize mucormycosis. in this work, we identified four adp-ribosylation factor (arf)-encoding genes (arf1-arf4) and study their role in the morphogenesis and virulence. arfs are key regulators of the vesicular trafficking process and are associated with both growth and virulence in fungi. arf1 and arf2 share 96% identity and arf3 and arf4 share 89% identity, which suggests that the g ...	2017	29264641
identification and characterization of diacylglycerol acyltransferase from oleaginous fungus mucor circinelloides.	acyl-coa:diacylglycerol acyltransferase (dgat) is a pivotal regulator of triacylglycerol (tag) synthesis. the oleaginous fungus mucor circinelloides has four putative dgats: mcdgat1a, mcdgat1b, mcdgat2a, and mcdgat2b, classified into the dgat1 and dgat2 subfamilies, respectively. to identify and characterize dgats in m. circinelloides, these four genes were expressed in saccharomyces cerevisiae h1246 (tag-deficient quadruple mutant), individually. tag biosynthesis was restored only by the expres ...	2018	29260551
mucor circinelloides induces platelet aggregation through integrin αiibβ3 and fcγriia.	thrombosis is a hallmark of the fatal fungal infection mucormycosis. yet, the platelet activation pathway in response to mucormycetes is unknown. in this study we determined the platelet aggregation potential of mucor circinelloides (m. circinelloides) nrrl3631, characterized the signaling pathway facilitating aggregation in response to fungal spores, and identified the influence of the spore developmental stage upon platelet aggregation potential. using impedance and light-transmission aggregom ...	2018	29297721
entomophthoromycosis and mucormycosis as causes of pneumonia in vietnamese potbellied pigs.	two vietnamese potbellied pigs ( sus scrofa) had respiratory disease and, on autopsy, both pigs had large masses in the lungs and thoracic cavity. microscopically, pulmonary and pleural masses contained large areas with hyphae surrounded by hypereosinophilic cellular debris rimmed by abundant eosinophils, lymphocytes, plasma cells, and histiocytes with occasional multinucleate giant cells. the hypereosinophilic debris usually formed tight cuffs, or "sleeves" around the hyphae, compatible with sp ...	2018	29034814
a protein complex bearing an oxidase with napthalene dihydrodiol dehydrogenase activity is induced in mucor circinelloides strain yr-1 during growth on polycyclic aromatic compounds.	fungi are organisms capable of growing in a myriad of conditions and respond to counteract environmental cues. several locations in the world are polluted with oil and its derivatives, and some microorganisms tolerant to these compounds have been isolated. some fungi can grow in the presence of molecules such as polycyclic aromatic hydrocarbons as sole carbon sources. in this report, we further characterized the induced enzymes with phenanthrene from mucor circinelloides yr-1 strain, isolated fr ...	2018	28975438