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dermatophyte agents in the city of são paulo, from 1992 to 2002.dermatophytosis are superficial mycoses caused by fungi that can invade stratum corneum and keratinized tissues. in order to study the frequency of dermatophytes species and the clinical manifestations caused by these fungi, in são paulo, sp, brazil, the authors analyzed cultures isolated at the mycology laboratory from a selected population (15,300 out-patients of the hospital das clínicas, department of dermatology, faculty of medicine of university of são paulo) from january 1992 to june 2002 ...200314743665
in vitro antifungal activity of sertaconazole against 309 dermatophyte clinical isolates.three hundred and nine strains belonging to 11 species of dermatophyte moulds were tested against sertaconazole following mainly the national committee for clinical laboratory standards (m38-p) for filamentous fungi. however, several important factors such as the temperature (28 degrees c vs 35 degrees c) and time of incubation (4-10 d vs 21-74 h), have been modified. sertaconazole was active against all the clinically important dermatophyte moulds involved in human infections tested. overall ge ...200314998079
profile of dermatophyte infections in baroda.this is an attempt to find the species prevalence of various dermatophytes in patients with dermatophytosis in our hospital in baroda.200317642912
antidermatophytic activity of extracts from psoralea corylifolia (fabaceae) correlated with the presence of a flavonoid compound.extracts obtained from seeds of psoralea corylifolia showed several degrees of antifungal activity against trichophyton rubrum, trichophyton mentagrophytes, epidermophyton floccosum and microsporum gypseum by the disc diffusion method on a sabouraud dextrose agar (sda) medium. methanol extract of the seeds at 250 microg exhibited the maximum inhibition with a halo of 28 mm diameter. six different bands were obtained when the methanol extract was subjected to tlc. 13c nmr and mass spectra reveale ...200415036462
a comparative survey of serum androgenic hormones levels between male patients with dermatophytosis and normal subjects.fungal growth can be influenced by human physiological mediators such as androgenic hormones. the aim of this study was to investigate a possible relationship between androgenic hormones and susceptibility to dermatophytosis. to this purpose we measured the levels of testosterone, androstendione, and dehydroepianderosterone sulfate (dhea-s) in 60 male patients with dermatophytosis due to epidermophyton floccosum and trichophyton rubrum by enzyme link immunoassay. serum testosterone concentration ...200415118212
onychomycosis in central india: a clinicoetiologic correlation.onychomycosis is mainly caused by dermatophytes, but yeasts and nondermatophyte molds have also been implicated, giving rise to diverse clinical presentations. the etiological agents of the disease may show geographic variation. the aim of the present study was to isolate the causative pathogens and to determine the various clinical patterns of onychomycosis in central india.200415230887
intra- and interlaboratory study of a method for testing the antifungal susceptibilities of dermatophytes.the national committee for clinical laboratory standards (nccls) m38-a standard for the susceptibility testing of conidium-forming filamentous fungi does not explicitly address the testing of dermatophytes. this multicenter study, involving six laboratories, investigated the mic reproducibility of seven antifungal agents tested against 25 dermatophyte isolates (5 blinded pairs of five dermatophyte species per site for a total of 300 tests), using the method of dermatophyte testing developed at t ...200415243047
frequency and risk factors of dermatophytosis in students living in rural areas in eskişehir, turkey.our study included 2384 students from five villages around eskişehir, turkey. we asked every student for their personal identification and also for their sanitation in order to get an idea about dermatophytosis. samples taken from suspicious lesion were collected and inoculated onto sabouraud dextrose agar slants. for identification of fungi which were grown, macroscopic appearance of colonies, microscopic examination and biochemical tests were used. we found suspicious lesions in 245 (10.3%) an ...200415281399
[statistical study of dermatomycosis for 30 years (1968-1997) in sendai national hospital].a statistical study on dermatomycoses for the 30 years from 1968 to 1997 in the dermatologic section of sendai national hospital was carried out with the following results. the total number of dermatomycosis patients was 14,259 and accounted for 9.59% of all new outpatients during this period. these cases of dermatomycoses were composed of the following: dermatophytoses 10,656, candidiasis 3,287, malassezia infection 566, sporothrichosis 20, aspergillosis 7, and chromomycosis 1. annual changes i ...200415284829
pcr and pcr-rflp techniques targeting the dna topoisomerase ii gene for rapid clinical diagnosis of the etiologic agent of dermatophytosis.we have focused on the dna topoisomerase ii genes of several pathogenic fungi, and developed polymerase chain reaction (pcr) and pcr-restriction fragment length polymorphism (rflp) methods targeting this gene for identification of dermatophytes.200414757280
prevalence and risk factors for superficial fungal infections among italian navy cadets.limited studies on the prevalence and risk factors for superficial mycoses are available.200415459531
in vitro susceptibility testing of amorolfine in pathogenic fungi isolated from dermatomycosis patients in china.the antifungal susceptibility of isolates from chinese dermatomycosis patients to amorolfine was investigated following national committee for clinical laboratory standards (nccls) protocols. in total, 383 isolates were tested, including 132 strains from tinea pedis, 148 strains from tinea corporis/cruris, and 103 strains from onychomycosis. the minimum inhibitory concentration (mic) of amorolfine against dermatophytes ranged from 0.01 to 0.08 microg ml(-1). the mic(50) and mic(90) of amorolfine ...200415504124
in vitro evaluation of griseofulvin against clinical isolates of dermatophytes from isfahan.fifty dermatophyte isolates, recently obtained from clinical materials, belonging to trichophyton mentagrophytes, t. verrucosum, microsporum canis and epidermophyton floccosum were examined for their susceptibility to griseofulvin. the minimum inhibitory concentration (mic) values were obtained using the modified microdilution method. all 100% tested isolates had mic geometric mean at a concentration between 0.43 and 0.95 microg ml(-1) the mic(90)s and mic(50)s were 8 microg ml(-1) and <0.25-1 m ...200415601457
etiologic agents of dermatophyte infection in lebanon.dermatophytes are common and cause important human fungal infections in many parts of the world, including lebanon. the prevalence of these fungi, however, tends to vary with time and geographic location. in our region, studies on the prevalence of dermatophytoses and the distribution of the various dermatophyte species involved are rare. in lebanon, only one study was published on this subject over the last forty years.200415884683
antidermatophyte activity of ether extract of nigella sativa and its active principle, thymoquinone.the antifungal activity of ether extract of nigella sativa seed and its active principle thymoquinone was tested against eight species of dermatophytes: four species of trichophyton rubrum and one each of trichophyton interdigitale, trichophyton mentagrophytes, epidermophyton floccosum and microsporum canis. agar diffusion method with serial dilutions of ether extract of nigella sativa, thymoquinone and griseofulvin was employed. the incubation was carried out at 30 degrees c for 14 days. the di ...200515908151
antifungal susceptibilities of dermatophytic agents isolated from clinical specimens.the aim of this study was to investigate the susceptibility to four antifungal agents: ketoconazole, terbinafine, itraconazole and fluconazole, of the different species of dermatophyte strains isolated from clinical specimens. a total of 128 specimens were collected from toe nail, foot, inguinal region, trunk, hands and head. the dermatophytes tested included trichophyton rubrum 108 (84.4%), trichophyton mentagrophytes 11 (8.6%), epidermophyton floccosum 5 (3.9%), microsporum canis 2 (1.5%) and ...200516048753
dermatophytes growth curve and in vitro susceptibility test: a broth micro-titration method.the introduction of systemic antifungal drugs which act upon different targets is the main issue of the in vivo antifungal resistance control. different factors, such as growth curve phase, quality of the specimen, quantity of the inoculum, temperature, ph, culture medium composition, incubation duration and solvent, are believed important factors affecting minimum inhibitory concentration (mic) value to most of the antifungal agents. we assayed an in vitro susceptibility test with 40 isolates o ...200516110777
antifungal activity of drimane sesquiterpenes from drimys brasiliensis using bioassay-guided fractionation.this study describes the antifungal effect of extracts and compounds isolated from drimys brasiliensis acting against dermatophytes.200516124945
[enzyme activities of dermatophytes isolated from different clinical samples by apizym method].dermatophytes' enzymes may have a role in chronic superficial infections. in this study it was planned to investigate the possible relationship between the enzymatic activities of the dermatophytes and acute or chronic course of the infections. dermatophytes were isolated from 58 (72%) out of 81 patients with superficial infections. the infections were divided into two groups as acute (< or = 6 weeks) or chronic (> 6 weeks). isolated fungi were identified by the classical methods and apizym meth ...200516128029
a study of dermatophytosis in south west of iran (ahwaz).two hundred and seventy nine patients suspected of having fungal lesions were examined. skin scrapping, hair samples and nail clippings were collected from patients. direct and culture examinations were performed for all samples. about 115 cases of examined subjects had dermatophytosis. dermatophytosis occurred mainly in adults males (20-29 years). tinea cruris (24.3%) was the most common type of dermatophytosis followed by tinea pedis (16.5%), tinea corporis (14.8%), tinea ungium (13%), tinea c ...200516160764
onychomycosis in adana, turkey: a 5-year study.onychomycosis results from invasion of the nail plate by dermatophytes, yeasts or mould species of fungi. this report documents the etiological agents of onychomycosis in adana, turkey.200516207188
onychomycosis in children: a survey of 46 cases.this is a retrospective study of the agents, clinical aspects, sources of infection and therapy of onychomycosis in children. in the period 1989-2000, we observed 46 consecutive children, until 16 years of age with onychomycosis (29 boys, 17 girls, mean age 10.8 years). dermatophytes were isolated in 30 cases (trichophyton rubrum in 22 cases, trichophyton mentagrophytes in five, epidermophyton floccosum in two and trichophyton violaceum in one) and candida spp. in 16, associated with trichophyto ...200516262881
role of chrysosporium keratinophillum in the parasitic evolution of dermatophytes.anti-dermatophytic activity of chrysosporium keratinophillum against species of the genera trichophyton, microsporum and epidermophyton floccosum was tested in vitro. when c. keratinophillum and different species of dermatophytes were inoculated on sabouraud's dextrose agar plates 2 cm apart, no antagonistic effect of c. keratinophillum on the mycelial growth of dermatophytes was observed. however, conidia production was not observed on the hyphae of trichophyton rubrum, trichophyton tonsurans a ...200516262884
use of magnetic beads to extract fungal dna.authors compare two methods of extracting dna from different fungi: the classic method with phenol/chloroform (p/c) and that with magnetic beads. both were tested on candida albicans and cryptococcus neoformans var. neoformans, belonging to the yeast group and microsporum canis, m. gypseum, trichophyton rubrum, t. interdigitale, t. ajelloi, epidermophyton floccosum, belonging to the dermatophytes group. extraction products underwent polymerase chain reaction (pcr) fingerprinting with the appropr ...200515679657
characteristics of dermatophytoses among children in an area south of tehran, iran.the aim of this study was to determine the prevalence and etiological agents of dermatophytoses, and also their distribution according to age, gender, and body site among children in an area south of tehran. a total of 382 children aged </=16 years suspected to have dermatophytic lesions were examined over a period of 3 years (1999-2001). the incidence rate of dermatophytoses was 6.6 per 100 000 person-years. trichophyton violaceum was the most frequent isolate (28.3%) followed by microsporum ca ...200515679663
prevalence of tinea capitis and tinea pedis in barcelona schoolchildren.although dermatophytoses can appear at any age, some types are particularly prevalent in children. there are no prior data on the prevalence of tinea capitis and tinea pedis in barcelona, spain. to identify the prevalence of tinea in school children in the area with the highest immigrant population in this city, a cross-sectional study was performed. a second objective was to identify the etiologic agent to study the possibility of the introduction of foreign dermatophyte species and to evaluate ...200515702042
comparison of disk diffusion method and broth microdilution method for antifungal susceptibility testing of dermatophytes.the use of the agar diffusion neo-sensitabs method to determine antifungal susceptibility of 59 isolates of dermatophytes, namely epidermophyton floccosum, microsporum canis, m. gypseum, trichophyton mentagrophytes, t. rubrum and t. tonsurans to clotrimazole (clz), itraconazole (itz) and terbinafine (tbf) is described. results obtained are compared to the minimum inhibitory concentrations (mic) determined by an adaptation of the nccls-m38-a procedure. using the diffusion method, all strains show ...200515712608
dermatophyte infections in the ljubljana region, slovenia, 1995-2002.we studied dermatophyte infections in patients, examined in the mycological laboratory of the department of dermatology, university medical centre ljubljana during the period 1995-2002. a total of 42,494 samples were collected from 33,974 patients suspected to have dermatomycosis. dermatophytes were identified in 8286 (71.2%) positive cultures. microsporum canis was the most frequent dermatophyte isolated (46.8%), followed by trichophyton rubrum (36.7%), t. mentagrophytes var. interdigitale (7.9 ...200515842334
nine male cases of tinea genitalis.nine cases of tinea genitalis observed in siena and terni (italy) between 1988 and 2003 are reported because of their infrequency. the patients were males ranging in age from 23 to 45 years. the lesions, situated on the penis, glans and scrotum, were preceded by dermatophytosis in other sites (groin five cases; feet two cases; toenails two cases; hands in one; beard in another). mycological examination consisting of direct microscopy and culture led to isolation of trichophyton rubrum in five ca ...200515842338
a prospective epidemiologic survey on the prevalence of onychomycosis and dermatophytosis in male boarding school residents.dermatophyte infections and onychomycosis are not usually serious in term of mortality; however, they may have significant clinical consequences such as secondary bacterial infections, chronicity, therapeutic difficulties and esthetic disfigurement in addition to serving as a reservoir of infection. our aim was to determine the prevalence of onychomycosis and dermatophytosis in a selected high risk group, consisting of male boarding school residents. a total of 410 males inhabiting two houses we ...200515883717
onychomycosis in eastern nepal.onychomycosis, a fungal infection of the nail is responsible for up to 50.0% of all nail diseases. though, dermatophytes are most frequently implicated as the causative agents in onychomycosis, yeast and molds are increasingly recognized as causative pathogens. this study was aimed to know the clinical and mycological pattern of onychomycosis in eastern nepal. eighty-two clinically diagnosed patients of onychomycosis attending the dermatology outpatient department of a tertiary hospital over a p ...200617357634
the complete dna sequence of the mitochondrial genome of the dermatophyte fungus epidermophyton floccosum.we report here the complete nucleotide sequence of the 30.9-kb mitochondrial genome of the dermatophyte fungus epidermophyton floccosum. all genes are encoded on the same dna strand and include seven subunits of the reduced nicotinamide adenine dinucleotide ubiquinone oxireductase (nad1, nad2, nad3, nad4, nad4l, nad5, and nad6), three subunits of cytochrome oxidase (cox1, cox2, and cox3), apocytochrome b (cob), three subunits of atp synthase (atp6, atp8, and atp9), the small and large ribosomal ...200616450111
dermatophytoses in monterrey, méxico.in the present report we reviewed a total of 2397 cases of dermatophytosis from superficial cutaneous lesions between the years 1978 and 1990. the cases included were from the department of dermatology at the university hospital located in the city of monterrey, méxico. a total of 726 tinea pedis, 613 tinea unguium, 441 tinea capitis, 395 tinea corporis and 222 tinea cruris cases were observed. the most commonly isolated dermatophyte species was trichophyton rubrum (45%), followed by trichophyto ...200616466445
efficacy of topical griseofulvin in treatment of tinea corporis.tinea infections are among the most common dermatological conditions throughout the world. griseofulvin is a classical oral fungistatic antibiotic, active against epidermophyton floccosum, trichophyton and microsporum species, the causative fungi of tinea corporis. to evaluate the efficacy of topical griseofulvin in the treatment of tinea circinata using three different vehicles for drug delivery. sixteen patients with tinea circinata were instructed to apply either griseofulvin gel form in grou ...200616681816
in vitro antifungal activity of luliconazole against clinical isolates from patients with dermatomycoses.the in vitro antifungal activity of luliconazole, a novel topical imidazole, against pathogenic fungi implicated in dermatomycoses was studied. a total of 91 clinical isolates, consisting of 59 trichophyton rubrum isolates, 26 t. mentagrophytes isolates, 1 epidermophyton floccosum isolate, and 5 candida albicans isolates were tested by the broth microdilution method, employing lanoconazole, terbinafine, and bifonazole as reference drugs. the minimum inhibitory concentrations (mics) of luliconazo ...200616826352
tinea incognito in italy: a 15-year survey.tinea incognito or steroid modified tinea is a dermatophytic infection in which topical or systemic steroids, administered as a result of dermatological misdiagnosis or preexisting pathologies, have modified the clinical appearance of the fungal infection, transforming the typical ringworm and mimicking other skin diseases. this is a retrospective study of the agents, clinical aspects, sources of infection of 200 cases (98 males, 102 females, mean age 42 years) of tinea incognito, observed in si ...200616922789
mycological flora of the hammams, traditional turkish bath.traditional turkish baths, hammams, occupy a prominent place in public health in history and at present. it is well-known that baths are major sources of fungal skin infections, but according to our knowledge fungal flora of turkish hammams was not defined yet. in bolu, turkey, two hammams were open to public and for detecting fungal flora, we collected 209 samples from different part of floors, tools and screened for the fungal pathogens. from floors of the dressing rooms, trichophyton rubrum a ...200616922794
complexes of 2-thiophenecarbonyl and isonicotinoyl hydrazones of 3-(n-methyl)isatin. a study of their antimicrobial activity.cobalt(ii), nickel(ii), copper(ii) and zinc(ii) complexes of 2-thiophenecarbonyl and isonicotinoyl hydrazones of 3-(n-methyl)isatin (hl(1) and hl(2), respectively) were synthesized and characterized, being the crystal structures of hl(1), hl(2) and [ni(l(1))(2)].2chcl(3) elucidated by x-ray diffraction techniques. the in vitro antimicrobial activity of all these compounds was tested against several bacteria and fungi. hl(1)and its complexes exhibited a strong inhibition of the growth of haemophi ...200717070919
in vitro activity of citrus bergamia (bergamot) oil against clinical isolates of dermatophytes.recently, bergamot oil was shown to be a potent antifungal agent in vitro against clinically important candida species. in this study, the activities of bergamot natural essence and its furocoumarin-free and distilled extracts on dermatophytes such as trichophyton, microsporum and epidermophyton species were investigated.200717118937
analysis of the dermatophyte species isolated in the british isles between 1980 and 2005 and review of worldwide dermatophyte trends over the last three decades.infections of the skin, hair and nails by dermatophyte fungi are common in developed and developing countries alike. however, the species involved and the resulting clinical entities vary both geographically and with time. we have surveyed 15,333 dermatophytes obtained from primary isolations at the mycology reference laboratory, bristol, uk from 1980 through 2005. several striking trends in dermatophyte prevalence were apparent over this period. the relative frequencies of isolations of microsp ...200717365649
antibacterial and antifungal activity of cassia fistula l.: an ethnomedicinal plant.hexane, chloroform, ethyl acetate, methanol and water extracts from the flower of cassia fistula (an ethnomedicinal plant) were tested against bacteria and fungi. all the extracts exhibited antibacterial activity against gram-positive organisms with minimum inhibitory concentrations (mic) between 0.078 and 2.5 mg/ml. among the gram-negative bacteria, only pseudomonas aeruginosa was susceptible to the extracts. ethyl acetate crude extract was fractionated using chromatographic techniques. a cryst ...200717532583
five-hour diagnosis of dermatophyte nail infections with specific detection of trichophyton rubrum.a rapid two-step dna extraction method and a multiplex pcr for the detection of dermatophytes in general and trichophyton rubrum specifically were developed and evaluated with dna extracted from pure cultures and from clinically diseased nails. dna from the following dermatophytes was used: epidermophyton floccosum, microsporum audouinii, microsporum canis, microsporum gypseum, microsporum nanum, trichophyton mentagrophytes, trichophyton rubrum, trichophyton schoenleinii, trichophyton soudanense ...200717267633
species distribution and antifungal susceptibilities of dermatophytes during a one year period at a university hospital in turkey.dermatophyte infections have been considered to be a major public health problem in many parts of the world. the aim of this study was to determine the causative agents of dermatophytoses and their antifungal susceptibilities in a turkish university hospital, west of turkey. a total of 926 patients suspected to have dermatophytic lesions were examined over a period of 1 year (2001-2002). samples collected from skin, hair and nails were submitted to direct microscopical examination using koh and ...200717305776
epidemiology of dermatophytoses in a rural community in eastern nigeria and review of literature from africa.a total of 4,287 primary school children, comprising 1,740 males and 2,547 females in arochukwu local government area of abia state nigeria were examined for clinical signs of dermatophytoses. about 873 (20.4%), consisting of 505 males and 368 females had lesions consistent with dermatophytoses. the disease was more prevalent in males (29%) than females (14.4%) in a ratio of approximately 2:1 (p < 0.05). the infection rate increased from 16.8% in the 4-6 year age group to a peak of 28.1% in the ...200717657581
composition and antifungal activity of the essential oil of mentha cervina from portugal.the chemical composition of the essential oils obtained by hydrodistillation from the aerial parts of mentha cervina collected during the flowering and vegetative phases of the plants were investigated by gc and gc-ms. quantitative differences were observed in the compositions, particularly in the amounts of pulegone (12.9-79.6%) and isomenthone (8.7-77.0%). antifungal activity of the oils was evaluated by minimal inhibitory concentrations (mic) and minimal lethal concentrations (mlc) against ca ...200717680495
evaluation of microdilution and disk diffusion methods for antifungal susceptibility testing of dermatophytes.a total of 63 dermatophyte strains belonging to 6 species (trichophyton rubrum (16), t. mentagrophytes (16), t. tonsurans (20), t. violaceum (2), microsporum canis (7), and epidermophyton floccosum (2)) were tested for their in vitro susceptibility to a range of antifungal drugs using disk diffusion and clinical laboratory standards institute m38-a (clsi, formerly nccls) broth microdilution methods. the antifungals used were ciclopirox (cic), terbinafine (ter), griseofulvin (gre), fluconazole (f ...200717885958
a 7-year survey of dermatophytoses in crete, greece.dermatophytoses are of worldwide distribution. epidemiological studies concerning dermatophyte infections have been performed in many countries and differences in the incidence and the aetiological agents have been reported in different geographical locations. this study was undertaken to investigate the prevailing species of dermatophytes in the island of crete, greece, and their pattern of infection during a 7-year period (1997-2003). a total of 5544 samples obtained from 3751 patients with cl ...200717944710
antifungal activities of the essential oils in syzygium aromaticum (l.) merr. et perry and leptospermum petersonii bailey and their constituents against various dermatophytes.this study was carried out in order to investigate the potential of using plant oils derived from leptospermum petersonii bailey and syzygium aromaticum l. merr. et perry as natural antifungal agents. the antifungal effects of essential oils at concentrations of 0.05, 0.1, 0.15, and 0.2 mg/ml on the dermatophytes microsporum canis (kctc 6591), trichophyton mentagrophytes (kctc 6077), trichophyton rubrum (kccm 60443), epidermophyton floccosum (kccm 11667), and microsporum gypseum were evaluated u ...200717978807
isotopic response of fungal granuloma following facial herpes zoster infections - report of three cases.an isotopic response is the occurrence of a new skin disease at the site of another unrelated, healed skin disorder.200717988332
antifungal activity of clove essential oil and its volatile vapour against dermatophytic fungi.antifungal activities of clove essential oil and its volatile vapour against dermatophytic fungi including candida albicans, epidermophyton floccosum. microsporum audouinii, trichophyton mentagrophytes, and trichophyton rubrum were investigated. both clove essential oil and its volatile vapour strongly inhibit spore germination and mycelial growth of the dermatophytic fungi tested. the volatile vapour of clove essential oil showed fungistatic activity whereas direct application of clove essentia ...200724015106
anti-fungal activity of crude extracts and essential oil of moringa oleifera lam.investigations were carried out to evaluate the therapeutic properties of the seeds and leaves of moringa oleifera lam as herbal medicines. ethanol extracts showed anti-fungal activities in vitro against dermatophytes such as trichophyton rubrum, trichophyton mentagrophytes, epidermophyton floccosum, and microsporum canis. gc-ms analysis of the chemical composition of the essential oil from leaves showed a total of 44 compounds. isolated extracts could be of use for the future development of ant ...200716406607
fungal skin infections among prison inmates in abakaliki, nigeria.of 402 prison inmates screened for fungal skin lesions, 79 (19.7%) showed skin lesions, which proved mycologically positive by microscopy and culture. dermatophytes were responsible for skin lesions in 61 (77.2%) of the inmates, while non-dermatophytes accounted for 18 (22.8%) of the lesions. the dermatophytes recovered were mostly anthropophilic and included trichophyton rubrum 33 (41.8%); t. mentagrophytes var. interdigitale 3 (3.8%); t. tonsurans 3 (3.8%); t. violaceum 2 (2.5%); epidermophyto ...200818076595
atypical microsporum canis variant in an immunosuppressed child.a 10-year old boy with x-chromosomal adrenoleukodystrophy presented with scaly patches on the scalp and diffuse effluvium. he was on immunosuppressive therapy because of a chronic graft-versus-host-reaction after allogenic bone marrow transplantation. at home he had been in contact with cats, rabbits and guinea pigs. through wood light and koh examination, we confirmed the diagnosis of tinea capitis and started antimycotic therapy. the morphology of the culture first suggested epidermophyton flo ...200818081856
dna microarray based on arrayed-primer extension technique for identification of pathogenic fungi responsible for invasive and superficial mycoses.an oligonucleotide microarray based on the arrayed-primer extension (apex) technique has been developed to simultaneously identify pathogenic fungi frequently isolated from invasive and superficial infections. species-specific oligonucleotide probes complementary to the internal transcribed spacer 1 and 2 (its1 and its2) region were designed for 24 species belonging to 10 genera, including candida species (candida albicans, candida dubliniensis, candida famata, candida glabrata, candida tropical ...200818160452
rapid identification and differentiation of trichophyton species, based on sequence polymorphisms of the ribosomal internal transcribed spacer regions, by rolling-circle amplification.dna sequencing analyses have demonstrated relatively limited polymorphisms within the fungal internal transcribed spacer (its) regions among trichophyton spp. we sequenced the its region (its1, 5.8s, and its2) for 42 dermatophytes belonging to seven species (trichophyton rubrum, t. mentagrophytes, t. soudanense, t. tonsurans, epidermophyton floccosum, microsporum canis, and m. gypseum) and developed a novel padlock probe and rolling-circle amplification (rca)-based method for identification of s ...200818234865
dermatophytoses in outpatients attending the dermatology center of avicenna hospital in qazvin, iran.dermatophytosis is still being considered as one of the major public health problems in many parts of the world. to identify the prevalence and etiological agents of dermatophytoses, a study was carried out between 2004 and 2006. out of 1023 subjects suspected to have cutaneous mycoses, 348 (34%) patients were affected with dermatophytoses. the causative agents were identified macroscopically and microscopically after the clinical samples were subjected to potassium hydroxide examination and cul ...200818254753
[tinea capitis].tinea capitis is a widespread scalp infection in children caused by dermatophytes. in fact, it is the most common cutaneous mycosis in children but is uncommon in adults. the disease has been major public health concern for decades. some factors implicated in infection include poor personal hygiene, crowded living conditions, and low socioeconomic status. it can be caused by any pathogenic dermatophyte except for epidermophyton floccosum and trichophyton concentricum. trichophyton rubrum, the mo ...200818346430
antifungal activity of heterothalamus alienus metabolites.the chemical study of heterothalamus alienus gave rutin, spathulenol (1), (1r,7s)-germacra-4(15),5,10(14)-trien-1beta-ol (2), sakuranetin (3), padmatin 3-acetate (4), (2r,3r)-dihydroquercetin-7,3',4'-trimethyl ether (5), (2r,3r)-dihydroquercetin-7,4'-dimethyl ether (6), (2r,3r)-3-acetoxy-5,7,4'-trihydroxyflavanone (7), as the main components of an antifungal extract of the aerial parts of the plant. compound 2 showed moderate activity, with epidermophyton floccosum being the most susceptible spe ...200818386258
ultraviolet c inactivation of dermatophytes: implications for treatment of onychomycosis.onychomycosis responds to systemic antifungals and sometimes to topical lacquers, but alternative treatments are desirable. topical application of germicidal ultraviolet (uv) c radiation may be an acceptable and effective therapy for infected nails.200818410410
[investigation of the agents and risk factors of dermatophytosis: a hospital-based study].the aims of this study were the detection of distribution of dermatophyte species isolated from the clinical samples of patients with dermatophytosis and the evaluation of risk factors for the development of dermatophytosis. a total of 441 skin, nail and scalp/hair specimens obtained from 301 patients (151 were male; age range 2 months-80 years, median 42 years) and 884 foot and hand skin and nail specimens obtained from 221 control subjects (110 were male; age range 5-75 years, median 36 years) ...200818444566
dihydroisocoumarin from xyris pterygoblephara active against dermatophyte fungi.the ethanol extract from xyris pteygoblephara aerial parts was evaluated against five microorganism strains, by the microdilution and agar diffusion methods. extract fractionation led to the isolation of three compounds, whose structures were assigned by spectrometric data (1d and 2d nmr, ir, ms and uv) as (3r,4r)-(-)-6-methoxy-3,4-dihydro-3-n-pentil-4-acethoxy-1h-2-benzopyran-1-one (1), moronic acid and quercetin. the absolute configuration of 1 was defined by circular dichroism spectroscopy an ...200817870137
updates on the epidemiology of dermatophyte infections.the spectrum of dermatophytes isolated from skin lesions had changed in last 70 years. before the second world war in germany, microsporum audouinii and epidermophyton floccosum ranked the first, whereas trichophyton rubrum is the most common dermatophyte since the fifties of last century, accounting for 80-90% of the strains, followed by t. mentagrophytes. this evolution is typical for central and north europe and it needs to be connected with the increase in the incidence of tinea pedis. in co ...200818478365
[a case of inflammatory tinea corporis by epidermophyton floccosum].we report a case of inflammatory tinea corporis by epidermophyton ( e. ) floccosum. a 73-year-old man visited our hospital with an eruption on his left arm where he wore his wristwatch. he had treated himself with a topical steroid. on koh preparation, many hyphal elements were observed in the scale. histological findings revealed granulomatous inflammation around hair follicles and positive fungal elements by pas and grocott staining in these follicles. topical luliconazole and oral terbinafin ...200818689972
comparison between the standardized clinical and laboratory standards institute m38-a2 method and a 2,3-bis(2-methoxy-4-nitro-5-[(sulphenylamino)carbonyl]-2h-tetrazolium hydroxide- based method for testing antifungal susceptibility of dermatophytes.in this study, we determined the utility of a 2,3-bis(2-methoxy-4-nitro-5-[(sulfenylamino)carbonyl]-2h-tetrazolium hydroxide (xtt)-based assay for determining antifungal susceptibilities of dermatophytes to terbinafine, ciclopirox, and voriconazole in comparison to the clinical and laboratory standards institute (clsi) m38-a2 method. forty-eight dermatophyte isolates, including trichophyton rubrum (n = 15), trichophyton mentagrophytes (n = 7), trichophyton tonsurans (n = 11), and epidermophyton ...200818832129
antimicrobial activity of iodoquinol 1%-hydrocortisone acetate 2% gel against ciclopirox and clotrimazole.commercially available topical formulations consisting of iodoquinol 1%-hydrocortisone acetate 2%, ciclopirox 0.77%, and clotrimazole 1%-betamethasone dipropionate 0.5% were assessed for their antimicrobial activity against cultures of micrococcus luteus, propionibacterium acnes, methicillin-resistant staphylococcus aureus (mrsa), pseudomonas aeruginosa, corynebacterium aquaticum, trichophyton mentagrophytes, malassezia furfur, microsporum canis, candida albicans, trichophyton rubrum, or epiderm ...200819055171
dermatophyte infections in patients attending a tertiary care hospital in northern italy.we studied dermatophyte infections in patients attending the dermatology outpatients clinic of s. matteo hospital pavia, italy, during the period 2004-2006. a total of 100 samples were collected from 95 patients; 97 dermatophytes and 3 keratinophylic fungi were isolated. trichophyton rubrum was the most frequent dermatophyte isolated (42.3%), followed by microsporum canis (31%), t. mentagrophytes (14.5%) and m. gypseum (9.2%). less frequently isolated were epidermophyton floccosum and t. violace ...200819123311
epidemiology of dermatophytoses in sfax, tunisia.the distribution of dermatophytes varies in different countries and geographical areas depending on several factors. to determine the frequency of aetiological agents and the clinical variants of dermatophytoses, we carried out a study between 1998 and 2007. out of 25 432 subjects suspected to have superficial mycoses, 9960 (39.2%) were affected with dermatophytoses; 14957 positive samples were obtained. the mean age was 35.7 years (range: 21 days to 97 years). sex ratio was 0.9. our patients we ...200919207834
epidemiological survey of dermatophytosis in tehran, iran, from 2000 to 2005.cutaneous fungal infections are common in tehran, iran, and causative organisms include dermatophytes, yeasts and non-dermatophyte molds. the prevalence of superficial mycosis infections has risen to such a level that skin mycoses now affect more than 20-25% of the world's population, making them the most frequent form of infection.200919293500
anti-fungal efficacy of polybutylcyanoacrylate nanoparticles of allicin and comparison with pure allicin.although garlic has been used in chinese traditional medicine for its medical properties for thousands of years, investigations into its mode of action are relatively recent. the purpose of this study was to evaluate the in vitro anti-fungal efficacy of the active principle of garlic, pure allicin and polybutylcyanoacrylate (pbca) nanoparticles (nps) loaded with allicin. pure allicin was prepared by reacting synthetic alliin with a stabilized process of the garlic enzyme alliinase. pbca nps were ...200919105898
dermatophyte infections in cairo, egypt.in this study, we examined dermatophyte infections in patients referred to the department of dermatology, el-houd el-marsoud hospital, cairo, during march 2004 to june 2005. of 506 patients enrolled in this investigation, 403 (79.6%) were clinically diagnosed as having dermatophytoses (age range 6-70 years; males 240; females 163). species identification determined by observation of their macroscopic and microscopic characteristics was complemented with sequencing of the internal transcribed spa ...200918972221
nb-002, a novel nanoemulsion with broad antifungal activity against dermatophytes, other filamentous fungi, and candida albicans.nb-002 is an oil-in-water emulsion designed for use for the treatment of skin, hair, and nail infections. the activity of nb-002 was compared to the activities of the available antifungal drugs against the major dermatophytes responsible for cutaneous infections, trichophyton rubrum, trichophyton mentagrophytes, epidermophyton floccosum, and microsporum spp., as well as 12 other genera of filamentous fungi. nb-002 consistently displayed fungicidal activity against all dermatophytes. the comparat ...200919433562
antibacterial and antifungal activity of flindersine isolated from the traditional medicinal plant, toddalia asiatica (l.) lam.the leaves and root of toddalia asiatica (l.) lam. (rutaceae) are widely used as a folk medicine in india. hexane, chloroform, ethyl acetate, methanol and water extracts of toddalia asiatica leaves and isolated compound flindersine were tested against bacteria and fungi.200919481384
causative agents of superficial mycoses in istanbul, turkey: retrospective study.the aim of the present study was to determine the percentage of agents, which can give rise to superficial fungal infections in istanbul, turkey. between 2000 and 2007, the clinical samples collected from 8,200 patients attending the outpatient dermatology clinic at mihrimahsultan medical center were examined by direct microscopy and culture. pathogen fungi were detected in 5,722 of the patients. of the isolates were 4,218 (74%) dermatophytes, 1,196 (21%) candida sp., 170 (3%) malassezia furfur, ...200919544086
onychomycosis infections in the middle black sea region, turkey.onychomycosis, a fungal infection of the nail, is caused by dermatophytes, yeasts, and nondermatophyte molds. the causative pathogen and incidence of onychomycosis depend on age, gender, geographic and climatic conditions, living habits, and immune status of the host.200919702980
heat resistance of dermatophyte's conidiospores from athletes kits stored in nigerian university sport's center.the incidence and heat resistance of conidiospores produced by dermatophytes isolated from athlete's kits (canvasses, stockings and spike shoes) stored in nigerian university sport's centre were investigated. epidermophyton floccosum, microsporum oudouinii, microsporum canis, trichophyton concentricum, trichophyton mentagrophytes and trichophyton rubrum were isolated and their incidence on the athlete's kits varied with the species and type of kits. among the isolates t. mentagrophytes, t. rubru ...200919388558
antifungal activity of eugenia umbelliflora against dermatophytes.antifungal activities of eugenia umbelliflora berg. (myrtaceae) were tested in vitro against a panel of standard and clinical isolates of human fungal pathogens (dermatophytes and opportunistic saprobes). methanol extracts of leaves and fruits of e. umbelliflora were separately prepared and partitioned, to yield dichloromethane (dcm), ethyl acetate (etoac) and aqueous fractions (aq). three compounds (1-3) were obtained from the dcm extract using chromatographic procedures. antifungal assays were ...200919831024
design, synthesis, and antifungal activity of triazole and benzotriazole derivatives.this study describes the design, synthesis and evaluation of a novel series of 1,2,4-triazole and benzotriazole derivatives as inhibitors of cytochrome p450 14alpha-demethylase (14dm). the chemical structures of the new compounds were confirmed by elemental and spectral ((1)h nmr, (13)c nmr, mass) analyses. compounds were designed by a generating virtual library of compounds and docking them into the enzyme active site. furthermore, they were found to have in vitro activity against microsporum c ...200918760508
epidermophyton fungal keratitis following laser-assisted subepithelial keratectomy.a 40-year-old man developed pain, decreased vision, and a corneal infiltrate 10 days after laser-assisted subepithelial keratectomy. treatment with conventional topical and systemic antibiotic agents did not improve the symptoms. approximately 2 weeks after surgery, the patient was referred to kim's eye hospital, presenting with counting fingers visual acuity, moderate anterior chamber reaction, and multifocal stromal infiltrates in the left eye. the corneal infiltrate findings were suggestive o ...200919969224
is moderation of protease production an adaptation of well-defined anthropization in dermatophytes?the protease activity of different isolates of dermatophytes representing different ecological groups namely geophilic, zoopahilic and anthropophilic, in their vegetative and sporulation growth phases were compared. unlike their geophilic and zoophilic counterparts, all the isolates of anthropophilic dermatophytes viz. trichophyton rubrum, t. mentagrophytes, t. tonsurans, t. violaceum and epidermophyton floccosum recorded reduced protease activity during artificially induced sporulation phase in ...201020090230
epidemiology of tinea pedis in cagliari, italy.observational study of all incident tinea pedis cases, realised from 2001 to 2007 at the dermatology clinic of the university of cagliari, italy, which is the main reference centre for dermatologic diseases in central southern sardinia.201020197740
onychomycosis.onychomycosis is a frequent nail disease caused by dermatophytes, yeasts, and nondermatophyte molds. trichophyton rubrum, t mentagrophytes, and epidermophyton floccosum are the most common etiologic agents worldwide. candida spp are the most frequent among the yeasts. diagnosis is corroborated by direct microscopic examination, culture, and histomycology with periodic acid-schiff stain. other new methods of diagnosis are discussed. treatment is based on oral antifungals: terbinafine, itraconazol ...201020347657
extraction, separation and isolation of volatiles from vitex agnus-castus l. (verbenaceae) wild species of sardinia, italy, by supercritical co2.isolation of volatile concentrates from leaves, flowers and fruits of vitex agnus-castus l. have been obtained by supercritical extraction with carbon dioxide. the composition of the volatile concentrates has been analysed by gc/ms. in all plant organs, the extracts are composed chiefly of alpha-pinene, sabinene, 1,8-cineole, alpha-terpinyl acetate, (e)-caryophyllene, (e)-beta-farnesene, bicyclogermacrene, spathulenol and manool. the main difference observed was in the content of sclarene, which ...201020397107
[dermatophytoses due to anthropophilic fungi in cadiz, spain, between 1997 and 2008].cutaneous fungal infections are a major public health problem. the distribution of the dermatophytoses varies between countries and geographical areas. the aim of this study was to determine the incidence, epidemiology, etiology, and clinical course of the dermatophytoses caused by anthropophilic fungi in cadiz, spain, over the past 12 years.201020398600
toxic effect of heavy metals on dermatophytes.summary for determining the toxic effect of heavy metals on dermatophytes, eight heavy metals were tested using colony diameter method. cadmium showed high toxicity effects on isolated fungi at minimal inhibitory concentration of 27 mug ml(-1) for trichophyton mentagrophytes and of 20 mug ml(-1) for epidermophyton floccosum, while iron enhanced dermatophytic growth. other heavy metals revealed variable effect on isolated fungi. susceptibility of e. floccosum to the activity of tested metals was ...201020406401
synergistic properties of methylxanthine with cadmium and mercury on dermatophytes.methylxanthine has many antimicrobial effects on different types of organisms. synergism actions between methylxanthine and cadmium or mercury on dermatophytes were tested.201020606961
evaluation of the association of superficial dermatophytosis and athletic activities with special reference to its prevention and control.this study was conducted to evaluate the association of superficial mycosis and athletic activities with special references to its prevention and control in tehran. participating in various kinds of sports can lead to direct and indirect exposures to and transmission of micro-organisms between athletes and also passive observers.201020636349
antimicrobial activity of confertifolin from polygonum hydropiper.confertifolin (6,6,9a-trimethyl-4,5,5a,6,7,8,9,9a-octahydronaphtho[1,2-c] furan-3 (1h)-one) was isolated from the essential oil of polygonum hydropiper l. (polygonaceae) leaves using column chromatography. confertifolin showed activity both in bacteria and fungi. the lowest mic for bacteria was observed against enterococcus faecalis (31.25 microg/ml). significant mic for fungi was observed against scopulariopsis sp (7.81 microg/ml), curvularia lunata (7.81 microg/ml), epidermophyton floccosum (7 ...201020645838
in vitro antifungal activity of indirubin isolated from a south indian ethnomedicinal plant wrightia tinctoria r. br.acalypha indica, cassia alata, lawsonia inermis, punica granatum, thespesia populnea and wrightia tinctoria are folklore medicines extensively used in the treatment of ringworm infections and skin related diseases in tamil nadu, india.201020691774
dermatophytes in republic of macedonia.(full text is available at http://www.manu.edu.mk/prilozi). a total of 1742 clinically suspected samples was examined for the incidence and distribution of dermatophytes in the republic of macedonia from june 2007 to jan. 2009. 600 dermatophytes were isolated and identified. in the studied period 9 different dermatophyte species were isolated, namely trichophyton rubrum (48.83%), microsporum canis (20%), trichophyton mentagrophytes var. interdigitale (16.83%), epidermophyton floccosum (4.17%), t ...201020693949
superficial fungal infections seen at a tertiary health centre: clinical and mycological studies.superficial fungal infections are common in the tropics, partly due to the climate, relatively poor hygiene, and lifestyle of the people.201020931516
the spectrum of fungal infections with a special reference to dermatophytoses in the capital area of kuwait during 2000-2005: a retrospective analysis.fungal infections constitute a major health problem all over the world. signs and symptoms induced by various dermatophytic infections are difficult to distinguish clinically from each other. so, characterization by in vitro culture is required for appropriate diagnosis and treatment as well as to study the epidemiological characteristics in a region.201019921463
dynamics of dermatophytosis frequency in mexico: an analysis of 2084 cases.we analysed 15,101 biological samples from patients presenting with superficial mycoses who attended outpatient services over a 10-year period. scale samples were processed for direct microscopic examination with 15% koh and cultured on sabouraud glucose agar plus chloramphenicol and cycloheximide. laboratory examination confirmed 4,709 cases of superficial mycosis (31.18%), of which 2,084 (44.26%) were dermatophytoses. the species most frequently encountered was trichophyton rubrum (71.2%), fol ...201019886762
comparison of the in vitro activity of terbinafine and lanoconazole against dermatophytes.the objective of this study was to compare the antifungal activity of terbinafine (terb) with that of lanoconazole (lan). test isolates, which were clinical isolates of japanese origin, included 10 strains each of trichophyton rubrum, t. mentagrophytes and epidermophyton floccosum. the minimum inhibitory concentration (mic) of terb and lan against each dermatophyte isolate was determined according to the clinical and laboratory standards institute microbroth methodology, m38-a2. minimum fungicid ...201019422522
dermatophyte species in superficial mycoses in the kraków district, poland in the years 1972-2007.considerable changes in the dermatophyte spectrum have been observed in the past century. hence, many authors point out the necessity of performing periodical overviews of the mycological flora producing mycoses in humans in a given area. analysis of dermatophyte species was performed, which were isolated from the lesions in patients suspected of superficial mycosis and referred to the department of mycology. the materials were isolated from patients suspected of superficial mycosis from kraków ...201019298357
study of onychomycosis in isfahan, iran.a mycological study was undertaken in 488 patients suspected of onychomycosis in isfahan, a large province of iran, to gain more insight into the prevalence and aetiology of this infection. direct microscopy of the nail clips was positive in 194 (39.8%) and fingernail onychomycosis was recognised in 141 (72.7%) and toenail onychomycosis in 53 (27.3%) cases. as agents of onychomycosis, yeast were detected in 112 (57.7%), dermatophytes in 27 (13.9%) and non-dermatophyte fungi in 55 (28.4%) patient ...201019298358
molecular typing of epidermophyton floccosum isolated from patients with dermatophytosis by rapd-pcr.we evaluated the ability of randomly amplified polymorphic dna (rapd) to type epidermophyton floccosum isolates recovered from patients with dermatophytosis originating from different regions of iran. a total of 13 clinical isolates of e. floccosum obtained from iranian patients were analyzed by rapd with 7 arbitrary primers (opn16, opd18' opu15, opx19, r28, opa04 and opaa17). among the applied primers, opn16 produced banding patterns from all the isolates. in addition, some of the isolates had ...201020967787
chemical composition and biological assays of essential oils of calamintha nepeta (l.) savi subsp. nepeta (lamiaceae).aerial parts of wild calamintha nepeta (l.) savi subsp. nepeta growing spontaneously on the mediterranean coast (sardinia island, italy) and on the atlantic coast (portugal) were used as a matrix for the supercritical extraction of volatile oil with co(2). the collected extracts were analysed by gc-fid and gc-ms methods and their compositions were compared with that of the essential oil isolated by hydrodistillation, but the differences were not relevant. a strong chemical variability was observ ...201020981614
composition of a volatile extract of eryngium duriaei subsp. juresianum (m. laínz) m. laínz, signalised by the antifungal activity.the composition of a volatile extract of eryngium duriaei subsp. juresianum, signalised by the antifungal activity (mic values=0.16-0.32 μl ml(-1)) against several dermatophyte species (trichophyton mentagrophytes, t. rubrum, epidermophyton floccosum; t. verrucosum, t. mentagrophytes var interdigitale, microsporum canis and m. gypseum) was established following a combined methodology of gc, gc-ms and an exclusive (13)c nmr technique that does not require prior isolation of compounds. twenty-five ...201021036502
elevation of antidermatophytic action of mefenamic acid by cobalt ions.to evaluate the antifungal property of mefenamic acid, which is a member of non-steroidal anti-inflammatory drugs (nsaids) group.201021189903
studies on antidermatophytic activity of waste leaves of curcuma longa l.during antidermatophytic screening of some essential oils, curcuma longa l. exhibited the strongest antifungal activity, completely inhibiting the mycelial growth of ringworm, caused by the fungi- microsporum gypseum and trichophyton mentagrophytes. the essential oil from leaves of curcuma longa was fungicidal at 2.5 μl/ml at which it tolerated heavy doses of inoculum. the fungicidal activity of the oil was thermostable up to 80 °c and self life up to 24 months in storage. the oil also showed a ...201023572967
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