Publications

evaluation of the inhibitory effect of n-acetyl-l-cysteine on babesia and theileria parasites.	n-acetyl-l-cysteine is known to have antibacterial, antiviral, antimalarial, and antioxidant activities. therefore, the in vitro inhibitory effect of this hit was evaluated in the present study on the growth of babesia and theileria parasites. the in vitro growth of babesia bovis, babesia bigemina, babesia divergens, theileria equi, and babesia caballi that were tested was significantly inhibited (p < 0.05) by micromolar concentrations of n-acetyl-l-cysteine. the inhibitory effect of n-acetyl-l- ...	2017	28655583
molecular identification and characterization of piroplasm species in hokkaido sika deer (cervus nippon yesoensis), japan.	babesia and theileria species are tick-borne protozoan parasites that have a veterinary and zoonotic importance. in order to investigate the prevalence and genetic diversity of these parasites, a total of 269 sika deer blood dna samples collected from hokkaido, japan, were examined for babesia and theileria species by touch-down pcr targeting the 18s rrna gene. reverse line blot (rlb) hybridization was then used to detect 12 piroplasm species. the results revealed that 95.5% (257/269), 94.1% (25 ...	2017	28651947
molecular identification and antigenic characterization of babesia divergens erythrocyte binding protein (bdebp) as a potential vaccine candidate.	host cell invasion is the only step where babesia parasites are extracellular, and their survival is menaced during this step. therefore, interfering with this critical stage is a target for an anti-babesia intervention strategy. in this regard, recombinant protein encoding babesia divergens erythrocyte binding protein (bdebp) was produced in escherichia coli in the current study, and its antiserum was prepared in mice for further molecular characterization. western blotting and indirect fluores ...	2017	28743470
a novel flow cytometric application discriminates among the effects of chemical inhibitors on various phases of babesia divergens intraerythrocytic cycle.	human babesiosis is a global emerging infectious disease caused by intraerythrocytic parasites of the genus babesia. its biology has remained largely unexplored due to a lack of critical tools and techniques required to define the various stages and phases of the parasite's cycle in its host rbc and the interplay between host and parasite. this article presents a powerful set of tools combining stage synchronization of the parasite with a platform that encompasses both a flow cytometric evaluati ...	2017	28207983
prevalence and distribution of borrelia and babesia species in ticks feeding on dogs in the u.k.	ticks were collected during march-july 2015 from dogs by veterinarians throughout the u.k. and used to estimate current prevalences and distributions of pathogens. dna was extracted from 4750 ticks and subjected to polymerase chain reaction and sequence analysis to identify borrelia burgdorferi sensu lato (spirochaetales: spirochaetaceae) and babesia (piroplasmida: babesiidae) species. of 4737 ticks [predominantly ixodes ricinus linneaus (ixodida: ixodidae)], b. burgdorferi s.l. was detected in ...	2017	28846148
high-resolution melting pcr assay, applicable for diagnostics and screening studies, allowing detection and differentiation of several babesia spp. infecting humans and animals.	the goal of the study was to design a single tube pcr test for detection and differentiation of babesia species in dna samples obtained from diverse biological materials. a multiplex, single tube pcr test was designed for amplification of approximately 400 bp region of the babesia 18s rrna gene. universal primers were designed to match dna of multiple babesia spp. and to have low levels of similarity to dna sequences of other intracellular protozoa and babesia hosts. the pcr products amplified f ...	2017	28795223
molecular evidence of a new babesia sp. in goats.	in this study, a novel babesia sp. infecting goats was detected and its phylogenetic relationship to related species was determined. a total of 200 blood samples collected from sheep (n=78) and goats (n=122) were examined in the study. the v4 hypervariable region of the 18s rrna gene of the novel babesia sp. was amplified by pcr and analysed using a reverse line blot hybridization assay adapted for small ruminants. samples from seven goats hybridized to theileria/babesia catch-all and babesia ca ...	2017	28043378
possible transfusion-transmitted babesia divergens-like/mo-1 in an arkansas patient.	a patient with asplenia and multiple red blood cell transfusions acquired babesiosis infection with babesia divergens-like/mo-1 and not babesia microti, the common united states species. he had no known tick exposure. this is believed to be the first transfusion-transmitted case and the fifth documented case of babesia divergens-like/mo-1.	2017	28329282
splenic dysfunction from celiac disease resulting in severe babesiosis.	we describe a 79-year-old irish man who, because he had hyposplenism and splenic atrophy due to adult celiac disease, became critically ill from a severe babesia divergens infection. greater awareness of the possible consequences of splenic dysfunction from adult celiac disease, such as serious pneumococcal infections and babesiosis, is warranted.	2017	28286145