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temperature effect on the insecticidal activity of pyrethroids on triatoma infestans.1. the toxic effect of deltamethrin, asymmethrin, alphamethrin, cypermethrin and cypermethrin high cis in nymphs iii of triatoma infestans was lower at 28 than 16 degrees c. 2. prostration in treated nymphs iii occurred more rapidly at 28 than 16 degrees c. 3. symptoms of poisoning rapidly disappeared when nymphs iii intoxicated were moved from 16 to 28 degrees c. 4. nymphs iii exposed to low doses of pyrethroids at 28 degrees c, which did not show symptoms of poisoning, were rapidly affected wh ...19938103441
feeding and mating deterrency by sulfhydryl reagents in triatoma infestans.topical application of different sulfhydryl (sh) reagents in acetone solution resulted in a deterrence of the feeding activity of triatoma infestans nymph v. the ed50 for topical application of n-ethylmaleimide (nem), the more active compound assayed, was 8.9 micrograms/insect. this effect showed a dose-dependent reversibility. we also observed feeding deterrency when a gauze cloth impregnated with nem was located between the food source and the nymphs. after topical nem treatment, mating deterr ...19938094591
[the importance of the peridomicile in a program for the elimination of triatoma infestans]. 19937855360
[operative indicators for a program for the elimination of triatoma infestans]. 19937855359
[guide for certification of triatoma infestans elimination in the south cone]. 19937855354
critical electrolyte concentration and dispersion of birefringence in heterochromatin and euchromatin of copper sulfate-treated specimens of triatoma infestans klug.binding of toluidine blue molecules to dna phosphates under mg2+ competitive staining conditions and dispersion of birefringence were studied in heterochromatin and euchromatin of malpighian tubule cells of the hemipteran, triatoma infestans; 24 h after treatment with copper sulfate. the objective was to detect whether copper binds to chromatin dna, affecting the critical electrolyte concentration (cec) value and the chromatin anisotropy in these chromatin types. a decrease in cec values as meas ...19948030384
hydrocarbon biosynthesis in triatoma infestans eggs.triatoma infestans eggs are shown to synthesize hydrocarbons. radio-gas chromatography was used to demonstrate metabolism of [1-14c]propionate into precursor methyl-branched fatty acids and into methyl-branched hydrocarbons in t. infestans eggs. these reactions have not been demonstrated previously in insect eggs. an in vivo study showed that hydrocarbons are also transported to eggs by the hemolymph. inhibition of hydrocarbon synthesis by sodium trichloroacetate (natca) was correlated with redu ...19948167362
inhibition of cuticular lipid synthesis and its effect on insect survival.a new approach to insect control--using sodium trichloroacetate (natca) to inhibit synthesis of the hydrophobic cuticular lipids that protect insects from dehydration--was tested on triatoma infestans. in vivo and in vitro studies of incorporation of radioactive precursors showed diminished cuticular hydrocarbon synthesis after natca treatment. thin layer chromatography and scanning electron microscopy showed disruption of the cuticular lipid layer of natca-treated insects, which also have incre ...19948167361
bloodmeal size and nutritional status of triatoma infestans under natural climatic conditions. 19948161837
nervous system of triatoma infestans.the anatomy of the adult nervous system of the haematophagous bug triatoma infestans has been studied by means of dissections and histology. the central nervous system comprises three nervous masses: the brain+suboesophageal ganglion, the prothoracic ganglion, and the posterior fused ganglion (meso+metathoracic+abdominal ganglia). the form of the brain is determined by the tubular head and the highly developed muscles of the pharyngeal pump. the prothoracic ganglion is located near the posternum ...19947932774
effect of gsh depletion mediated by l-bso on the insecticidal activity of ddt and fenitrothion in triatoma infestans (hemiptera).l-bso injected at a sublethal dose in nymph v of triatoma infestans produces a transient gsh depletion in the abdomen. a depletion of the total content of gsh was observed in nymph ii fed with a sublethal concentration of l-bso. either ingestion or injection pretreatments of t. infestans nymph v with sublethal concentrations of l-bso produced a slight synergism on the toxicity of fenitrothion. feeding administration of a sublethal concentration of l-bso to nymphs ii of t. infestans resulted in a ...19948061956
nos is present in the brain of triatoma infestans and is colocalized with cck.immunohistochemistry was used to establish the presence of nitric oxide synthase (nos) in the central nervous system of triatoma infestans which is the main vector for chagas' disease in argentina and neighbouring countries. in addition, we have investigated the presence of cholecystokinin (cck) and studied the possible coexistence of these molecules. the results show nos-like immunoreactivity (li) in neurones of the soma rind of the protocerebrum, the optic lobe and in the lateral part of the s ...19947535578
chagas disease in north-west argentina: risk of domestic reinfestation by triatoma infestans after a single community-wide application of deltamethrin.the risk of domestic reinfestation by triatoma infestans after a single community-wide application of delta-methrin (2.5% suspension concentrate at 25 mg active ingredient/m2) was studied in amamá, north-west argentina, where no insecticide spraying had been done by official control services. the percentage of infested houses fell from 88% before spraying in 1985 to nil during the 6 months after spraying, and thereafter increased from 5% in 1986 to 96% before a second treatment in 1992, fitting ...19948153989
sexual behaviour and stridulation during mating in triatoma infestans (hemiptera: reduviidae).factors affecting mating behaviour in the bug triatoma infestans were quantitatively studied in the laboratory. experimental conditions were established so that the probability of copulation increased with the time elapsed since the first adult meal. copulatory attempts by males did not vary as a function of time, but rejections by females became significantly less frequent with the post feeding time. non-receptive females displayed four types of rejection behaviour, i.e. flattening, stridulatio ...19948524065
[susceptibility of triatoma infestans to various strains of trypanosoma cruzi isolated from chagasic patients from the triângulo mineiro].this data evaluated the susceptibility of t. infestans to seven different strains of t. cruzi from chagasic patients of the triângulo mineiro, isolated and adapted in swiss mice. a total of 200 bugs were fed in mice in acute phase injected with the different strains of t. cruzi and at each 30 days, during 5 months, their feces were obtained by abdominal compression or spontaneous dejection. the overall positivity of t. infestans infection with these different strains of t. cruzi was 62.08%. ther ...19947855366
[epidemiology of chagas' disease in chile. serological follow-up of 1,906 inhabitants from an endemic rural area, iv region, 1991-1993].the iv region (29 degrees 30'-32 degrees 13' south lat.) is located in the center of the geographical area of distribution of chagas' disease in chile. triatoma infestans is the main and almost exclusive vector of trypanosoma cruzi in this country. the mean prevalence rate of t. cruzi human infection in urban and periurban sections of iv region is 24.7%. to assess the impact of anti-t. infestans activities, by means of health education and sprayings of dwellings with insecticides, carried out in ...19947654291
interstitial pneumonitis induced in guinea pigs by triatoma infestans antigens.data concerning the experimental induction of hypersensitivity pneumonitis in guinea pigs with a triatoma infestans antigen are presented. glycoproteins obtained from the chitinous structures of t. infestans (79 kd + 11 kd) were aerosolized daily to guinea pigs during 7 weeks. the presence of specific antibodies (igg and ige) was detected by serological techniques; histopathological studies of the lungs showed interstitial infiltrates of macrophages and t-cells. single non-necrotizing granulomas ...19947850034
[compared dynamics of triatoma infestans cohorts in residential environment].triatoma infestans is an insect with a high colonization capacity, capable of maintaining populations near saturation levels in different environments. however, it has a great lability in its population parameters when exposed to variable environmental conditions. the aim of this work was to explore the response of two cohorts with 4 initial egg densities, in two environmental conditions ("shelter" at 24 +/- 2.7 degrees c and 73 +/- 7% of relative humidity and "room" at 16.9 +/- 2.1 degrees c an ...19947597346
the control of triatoma infestans in mambaí, goiás-brasil. 19947948935
evaluation of the chagas' disease vector control program in minas gerais, brazil, with special reference to triatoma sordida.as of 1979, data gathered by the chagas' disease control program (cdcp) in the brazilian state of minas gerais indicated that triatoma infestans was the chagas' disease vector most commonly found in and around human dwellings. a decade later, however, this picture had changed, presumably as a result of control efforts; few specimens of t. infestans were collected; and triatoma sordida had become the most commonly collected vector insect. the aim of the work reported here was to assess the effect ...19947951364
[population interactions between triatoma infestans and triatoma sordida].populations of t. infestans and t. sordida were studied between september 1988 and april 1989 with a view to discovering if any kind of interaction took place between them while they lives together in the same experimental unit and exploited the same food resource (chicken). the initial age structure for each species was: 27 n1, 7 n2, 11 n3, 3 n4, 8 n5, 4 males and 10 females. the population dynamics nutritional status, predation and gregarious behavior were estimated by means of a monthly censu ...19947997824
enzyme polymorphism among triatoma infestans (hemiptera: reduviidae) colonies.allozyme variability in populations of the chagas's disease vector triatoma infestans (klug) was investigated by means of starch gel electrophoresis. samples were taken from nine laboratory colonies established with individuals collected at different localities across the range of this insect in south america. zymograms for proteins coded by a total of 17 loci were obtained. allele frequencies, proportion of polymorphic loci (p), mean heterozygosity per locus (h), similarity (s), and identity (i ...19957608917
biochemical, immunological, and biological characterization of trypanosoma cruzi populations of the andean north of chile.twenty-one trypanosoma cruzi stocks isolated from triatoma infestans and humans of the chilean andean highlands were studied at the genotypic level by schizodeme and molecular karyotype analyses, which allowed a clear distinction of the parasites from those hosts. a phenotypical characterization was performed by proteolytic activity after electrophoretic fractionation without discrimination among the stocks. metacyclic trypomastigotes obtained in vitro proved to be infective to swiss mice and th ...19957628560
baker's yeast, an attractant for baiting traps for chagas' disease vectors.we tested the attraction of volatile compounds, produced by the aerobic growth of saccharomyces cerevisiae on saccharose for triatoma infestans. for these tests, we exploited the behavioural characteristic of these haematophagous insects of dropping when searching for food. in olfactometer assays, yeast cultures activated and attracted bugs as effectively as a mouse. the attraction of the cultures was significantly reduced when the carbon dioxide released was partially eliminated using potassium ...19957649243
type of rectal contents and infectivity of domiciliary populations of triatoma infestans (hemiptera: reduviidae) in argentina.different types of rectal content in domiciliary populations of triatoma infestans (hemiptera: reduviidae: triatominae) were associated with differences in the degree of infection with trypanosoma (schizotrypanum) cruzi. field studies were carried out in a region of argentina (cordoba) where chagas' disease is endemic. the rectal contents of domiciliary t. infestans were classified by color (clear, yellow, or brown) and the number of infective metacyclic stages of t. cruzi. the seasonal percenta ...19957650696
field application of polymerase chain reaction diagnosis and strain typing of trypanosoma cruzi in bolivian triatomines.a new approach for direct identification and characterization of trypanosoma cruzi stocks in biological samples was tested for field applicability on an extensive sample of feces collected from triatomine vectors from four different species found in bolivia. the first step of the technique is polymerase chain reaction (pcr) amplification of the hypervariable region of kinetoplast dna minicircles of t. cruzi parasites. in this report, 345 fecal samples were analyzed and the pcr results were compa ...19957677221
mutagenicity, insecticidal and trypanocidal activity of some paraguayan asteraceae.the insecticidal, moulting inhibition and trypanocidal effects of crude extracts of 7 paraguayan asteraceae were evaluated on triatoma infestans and bloodstream forms of trypanosoma cruzi, respectively. both mutagenicity and toxicity were evaluated by sister chromatid exchange (sce) in human peripheral lymphocyte culture and by the lethality test of artemia salina. the ethanolic extracts from chromolaena christieana (stem and bark), achyrocline satureoides (leaves and flowers) and mikania cordif ...19957739225
karyotype evolution in holocentric chromosomes of three related species of triatomines (hemiptera-reduviidae).c-banded karyotypes, dna content and the male meiiotic process of triatoma platensis and triatoma delpontei are compared with those of triatoma infestans, the main vector of chagas disease in south america. these three species present the same diploid chromosome number 2n = 22 (20 autosomes + xx male/xy female). they also have several cytogenetic traits that differ from all other triatomines: large autosomes, c-heterochromatic blocks and meiotic heteropycnotic chromocenters formed by autosomes a ...19957780658
development of trypanosoma cruzi in triatoma infestans: influence of temperature and blood consumption.this work had 2 objectives. the first was to quantify trypanosoma cruzi development within triatoma infestans maintained at 2 different temperatures, using an experimental design that simulated the natural transmission process and, second, to learn how the vector blood consumption rate modifies the parasite's development. two hundred and three, fifth-stage nymphs of t. infestans were infected with the x-1 strain of t. cruzi (about 10(4) trypanosomes/ml blood), maintained at 20 and 28 c, and dail ...19957876960
laboratory comparison of feeding success among triatoma infestans, t. guasayana, and t. sordida (hemiptera: reduviidae).success in obtaining a blood meal and rapid access to hiding places after feeding are the principal requirements triatomines have as they colonize artificial ecotopes. feeding success and postfeeding location of 3rd and 5th instars of triatoma sordida (stål), of t. guasayana wygodzinsky & abalos, and t. infestans (klug) were studied in an experimental box in which triatomines and a chicken were left to interact overnight. the bird was enclosed in a glass cube, slightly raised to allow triatomine ...19957473611
[feeding and defecation dynamics of triatoma sordida].today, triatoma sordida is the most frequently captured triatomine in brazil. for a better understanding of its vectorial potential, its feeding dynamics and dejections behaviour were studied in comparison with triatoma infestans and p. megistus. the proportion of t. sordida and t. infestans that blood fed on anaesthetized rats did not differ significantly. there was no significant difference in the time elapse between release of the bugs and the initiation of blood feeding. the mean time betwee ...19957480912
evaluation of risk factors for house infestation by triatoma infestans in brazil.an active entomologic survey was conducted by a team of trained health workers in a rural area endemic for chagas' disease in central brazil. they used pyrethrum as a flushing agent and 4,232 houses were inspected for triatomine bugs both inside and in the immediate environs. houses with triatoma infestans or evidence of an established colony were identified and defined as infested houses (cases). the building and environmental characteristics of 161 randomly selected infested houses were compar ...19957485701
detecting domestic vectors of chagas disease: a comparative trial of six methods in north-west argentina.six methods for detecting domestic infestations by triatomine bugs were compared in the rural community of amamá, north-west argentina. an average of three pairs (range, 2-5 pairs) of sensor boxes and sheets of pink typing-paper were tacked to the walls of human sleeping areas in 45 houses for 30 days and then inspected by a two-man team. triatoma infestans bugs were collected in bedrooms by a different two-man team aided by a flushing-out agent both before and after application of sensing devic ...19957554021
[urban ecology of triatoma infestans in argentina. association between triatoma infestans and pigeon cotes].a study was undertaken in an urban area of the capital city of the province of san juan, argentina, in a housing complex of 768 flats distributed in buildings of 3 and 7 floors each surrounding an abandoned central winery. a total of 329 triatoma infestans were captured, 293 on 4 terraces of seven-floor-towers and 36 inside the winery, associated with the greater number of pigeons which nest in those places. the bugs were sheltered in the dung accumulated between the cement blocks used to floor ...19958539530
brindley's gland exocrine products of triatoma infestans.volatile exocrine products of the metathoracic brindley's glands in triatoma infestans (hemiptera: reduviidae) were obtained by dissection and by sampling the air passed over agitated live adults. isobutyric acid was the main component in the glands, together with isobutyl, isoamyl and amyl alcohols, 2-phenylethanol and other carboxylic acids and esters. isobutyric acid, isobutyl, isoamyl and amyl alcohols and ester were also found to be emitted into the air, apparently for defence. no volatile ...19958541592
residual effect of lambdacyhalothrin on triatoma infestans.insecticidal residual effect and triatomine infestation rates in houses of a community fumigated with lambdcyhalothrin (icon) are reported. no mortality was observed in 5th-instar triatoma infestans nymphs in 72-hr exposure test on three different surfaces, one month after fumigation for a dose of 31.5 mg am/m2. however, during post-exposure observation a mortality of 60% was recorded for those insect exposed on sprayed woodboard. the results observed with mud-containing treated walls, were mark ...19958544744
the interaction between poultry and triatoma infestans klug, 1834 (hemiptera: reduviidae) in an experimental model.this paper compares the predation pressure that ducks and chickens exert on triatomines. for the tests, these birds were placed in individual boxes together with a known number of triatoma infestans and left to interact from 6 p.m. till the next morning, involving a long lasting period of complete darkness limited by two short-term periods of semi-darkness. there was a shelter which could prevent the bugs from being predated. the number of live and dead triatomines was recorded, considering miss ...19958544747
a sialidase activity in the midgut of the insect triatoma infestans is responsible for the low levels of sialic acid in trypanosoma cruzi growing in the insect vector.trypanosoma cruzi expresses a unique trans-sialidase that is responsible for the transfer of sialic acid from host glycoproteins and glycolipids to mucin-like glycoprotein acceptors on the parasite surface. the enzyme and the sialic acid acceptors are present in the mammalian forms of the parasite and in the parasite forms that grow in axenic cultures, which correspond to the developmental stages found in the insect vectors. here we show that parasite forms growing in the vector triatoma infesta ...19958563150
existence of a sex pheromone in triatoma infestans (hemiptera: reduviidae): i. behavioural evidence.assembling behaviour associated with mating was investigated in triatoma infestans. the spatial distribution of both sexes was observed by video films, in the presence or absence of a copulating pair. males aggregated around copulating pairs. females did not exhibit this behaviour and their mean spatial density remained unaffected. spontaneous aggregation tendency was observed in males in the absence of a copulating pair, but the temporal course significantly differed from that observed in the p ...19958569481
existence of a sex pheromone in triatoma infestans (hemiptera: reduviidae): ii. electrophysiological correlates.the stimulus provided by a copulating pair of triatoma infestans significantly affects the electrical activity of the nervous system of triatoma infestans. electrophysiological recordings were performed on stationary adult males presented with stimuli of an air current carrying odors from males, females, non-copulating pairs and mating pairs. the electrophysiological response was characterized by the low frequency occurrence of biphasic compound impulses. a significant increase in the frequency ...19958569482
[positivity of xenodiagnosis, according to age, in persons with positive serology for chagas disease].an analytical study of positive xenodiagnosis (xd), according to age distribution, in people with a positive indirect hemagglutination test (ihat) for chagas' disease has been carried out. a total of 1,137 ihat for chagas' disease positive persons were submitted to two wooden xd boxes containing 7 triatoma infestans nymphs iii each. the positivity (%) of xd in the different age groups was: 0-9 year-old (60.8), 10-19 (35.8), 20-29 (31.5), 30-39 (28.7), 40-49 (30.0), 50-59 (34.3) and > or = 60 (43 ...19958573269
interaction between didelphis albiventris and triatoma infestans in relation to trypanosoma cruzi transmission.this paper attempts to prove if a high trypanosoma cruzi prevalence of opossums might be reached with few potential infective contacts. one non-infected didelphis albiventris to t. cruzi and 10 infected nymphs of triatoma infestans were left together during 23 hr in a device that simulated a natural opossum burrow. twenty-six replicates were performed using marsupials and triatomines only once. potentially infective contacts occurred in all the trials. from the 26 opossums used in trials, 54% di ...19958731363
triatoma infestans is more efficient than panstrongylus megistus in obtaining blood meals on non anaesthetized mice.we compared the influence of bug density in the capacity of triatoma infestans and panstrongylus megistus in obtaining blood meal in non anaesthetized mice. the regression analysis for increase in body weight (mg) versus density (no. of bugs/mouse) showed that in experiments with anaesthetized mice (am), no correlation was observed. in experiments with non anaesthetized mice (nam) the weight increase was inversely proportional to density. the regression slope for blood meal size on density was l ...19958731374
[evaluation of a triatoma infestans eradication program in rural dwellings from the iv region, chile, by means of serology for chagas disease in 0-10 years old children].the present study deals with the evaluation of the effectiveness of the measures employed in the control of triatoma infestans, the vector of trypanosoma cruzi infection (chagas' disease), in the iv region of chile through a serological follow up program. for this reason, a serological survey has been carried out in 2,783 children, from 0 to 10 years, who live in rural high endemic areas of the region. the sera were tested using an indirect hemagglutination test (ihat) and elisa techniques with ...19958762674
[xenodiagnosis in chronic chagas' disease. i. the sensitivity of panstrongylus megistus and triatoma infestans].from january 1986 to february 1994, 563 xenodiagnosis (xd) were applied in 563 chronic chagasic patients from different areas of brazil; 292 were women and 271 were men between 6 and 89 years (average: 41.4 +/- 14.7 years). to each xd 40 nymphs on the 4th stage were used: 20 from panstrongylus megistus (pm) and 20 from triatoma infestans (ti) in fast, during at least 14 days. the exam in each nymph was made 45 days after being applied on the patient, by observation in optical microscopy of the d ...19968768582
chemical constituents of essential oils of muña, bolivian plants traditionally used as pesticides, and their insecticidal properties against chagas' disease vectors.the composition of essential oils from two muña, bolivian medicinal plants, derived from minthostachys andina and hedomea mandonianum, were analyzed by gas chromatography/mass spectrometry. major differences were observed in their chemical composition. pulegone was the major component of h. mandonianum oil (44.6%) and m. andina oil (25.5%); menthone and isomenthone were around 33% of these oils. differences were also observed in their insecticidal activity against the chagas' disease vector, rho ...19968771455
genetic analysis of triatoma infestans following insecticidal control interventions in central bolivia. 19968790776
trypanosoma cruzi: study of the distribution of two widespread clonal genotypes in bolivian triatoma infestans vectors shows a high frequency of mixed infections.the detection of two widespread trypanosoma cruzi clonal genotypes (20 and 39) in feces of bolivian specimens of the vector triatoma infestans was performed by a combination of polymerase chain reaction and clone-specific dna hybridization. the hybridization pattern of 186 pcr positive samples cf t. infestans feces collected in two bolivian departments identified clone 20 in 74.2% and clone 39 in 63.4% of the triatomine bugs. for the first time, a high percentage (mean: 43.2 +/- 26%) of mixed in ...19968823244
reduction of triatoma infestans (hemiptera:reduviidae) experimental populations exposed to feeding inhibitors.we found that feeding behavior of triatoma infestans (klug) 5th-instar nymphs was blocked by topical application of the cis-isomers of methyl esters of n-substituted maleamic acids, synthesized in our laboratory. the antifeeding effect of these compounds was determined by exposing 5th-instar nymphs to impregnated filter papers. the greatest antifeeding effect was observed for the shortest alkyl chain of cis-n-substituted methyl maleamate (ec50: 2.47 micrograms/cm2 when the substituent was ethyl ...19968840690
host-feeding patterns of domiciliary triatoma infestans (hemiptera: reduviidae) in northwest argentina: seasonal and instar variation.blood meal sources of 1,964 triatoma infestans klug collected in bedrooms in 3 rural villages in northwest argentina were identified by agar double-diffusion tests. bugs were collected in september (1988, end of winter), october (1992), december (1988, spring), and march (1989, 1992, summer), and tested for human, dog, cat, chicken-duck, and goat-sheep serum antigens. from late winter to late summer, the percentage of domiciliary t. infestans that fed on humans decreased from 81 to 50-51%, where ...19968906900
comparative toxicity of deltamethrin and cis-permethrin on first instars of triatoma infestans (hemiptera: reduviidae).we evaluated the toxicity of deltamethrin and cis-permethrin at 3 temperatures on instars of triatoma infestans (klug). after treatment, temperature had an important effect on ld50s of both insecticides, which tended to decrease when temperature decreased. however, the effect of temperature on lt50s was small. cis-permethrin was slightly more lethal than deltamethrin only at 26 degrees c. lethal action of deltamethrin was faster than cis-permethrin at all temperatures assayed. the toxicity of bo ...19968906906
cross reactivity between the antigens of periplaneta americana and triatoma infestans.the cockroach periplaneta americana (pa) and the reduviid triatoma infestans (ti) are two very common insects in argentina that pollute indoor and outdoor environments. they are also strongly immunogenic in animals and in atopic men. several immunological methods such as serological absorption procedures, diffusion gel methods and capillary electrophoresis were applied in order to establish the existence of common glycoprotein epitopes in the cockroach and in the reduviid. the presence of specif ...19968959541
[domestic infestation by triatoma infestans and prevalence of trypanosoma cruzi seropositives in a rural area of the argentinian northeast].an epidemiological study was carried out from april 1991 to december 1993 to obtain a general view of t. cruzi domestic transmission in rural areas of san miguei department, corrientes. from 100 analyzed households, 50.0% was infested by t infestans (klug, 1834) and 1.0% by t. sordida (stal, 1859). domiciliary colonization by t. sordida is reported for first time in corrientes province. t. cruzi infection of t. infestans was 23.1%. indirect hemagglutination and indirect immunofluorescence antibo ...19969011879
use of the polymerase chain reaction for detecting trypanosoma cruzi in triatomine vectors.determination of the rate of trypanosoma cruzi infection in its triatomine vectors is an element in control programmes directed at reducing transmission of the organism to humans. traditionally, t. cruzi has been detected in these insects by microscopical examination of intestinal contents or excreta. the sensitivity of this laborious process has not been defined because of the lack of a bench-mark method against which microscopical examination could be compared. the purpose of this study was to ...19969015504
[use of new tools for controlling triatomines in different entomologic situations in the american continent].the field results of new tools for triatomine control developed under the sponsorship of who/tdr are reported: a) a slow-release "paint" containing malathion; b) fumigant cans containing cypermethrin and ddvp, and c) sensor boxes for the detection of triatomine infestations. field assays were performed in chile, honduras and paraguay against triatoma infestans, t. dimidiata and rhodnius prolixus, accordingly to a standard protocol designed by a who experts committee. preliminary 6 months post-tr ...19969026830
current spread of triatoma infestans at the expense of triatoma sordida in bolivia. 19969040844
urban ecology of triatoma infestans in san juan, argentina.this study was performed in an urban neighborhood of the capital city of the province of san juan, argentina. erected as a housing complex, the place consists of 768 flats distributed in buildings of three and seven floors each. a survey was carried out in 33% of the dwellings, enquiring about the number of triatoma infestans found indoors, stage of the bug development-nymph or adult- and how these insects had entered their homes. adult t.infestans were found on all floors; 163 people (64%) had ...19969070399
comparison of the blood meal size among triatoma infestans, t. guasayana and t. sordida (hemiptera: reduviidae) of argentina under laboratory conditions. 19968736098
[longevity of trypanosoma cruzi in the triatoma infestans].maintenance of trypanosoma cruzi infection in triatoma infestans was evaluated. seventy nine fourth instar nymphs were followed by 12 months, after being fed in infected mice. the bugs were examined every month, fecal smears were done to document t. cruzi infection then the bugs were fed in "clean" (uninfected) mice. thirty nine per cent of the bugs remained infected until death twenty three per cent cleared infection and remained uninfected until death and nineteen per cent alternated positive ...19968762648
trypanosoma cruzi: polymerase chain reaction-based detection in dried feces of triatoma infestans.pcr was employed to detect trypanosoma cruzi dna in triatoma infestans dry fecal spots collected on filter papers. both insects fed on experimentally infected monkeys and insects collected in a paraguayan endemic area for chagas' disease were examined. when the insects fed on a chronically infected monkey with low parasitemia as revealed by direct microscopic observation (dmo), t. cruzi was detected in the insect feces by pcr as soon as 2 days postfeeding. when the same experiment was performed ...19968654552
trypanosoma cruzi: involvement of intracellular calcium in multiplication and differentiation.the possible role of intracellular ca2+ level on trypanosoma cruzi differentiation was explored. the addition to epimastigotes of a triatoma infestans intestinal homogenate, which that triggers off the differentiation to the infective metacyclic form, induced a sudden rise in [ca2+]i from the basal value, 94 +/- 28 to 584 +/- 43 nmole/liter. this increase was not affected by the presence of egta in the medium. trypsin-treated intestinal homogenate did not alter the [ca2+]i of epimastigotes. calm ...19968682192
potential colonization of the peridomicile by triatoma guasayana (hemiptera:reduviidae) in santiago del estero, argentina.sylvatic triatomines might use the peridomicile as a 1st step in the process of domiciliation. therefore, we evaluated the capability of sylvatic species to colonize the peridomicile of a rural area in the province of santiago del estero, argentina. the research was carried out in 6 houses in the village of trinidad. the person per hour capture method was employed to determine the presence of triatomines in all the buildings (n = 44). dispersing adults were collected by means of light traps and ...19968699459
probability of infection with trypanosoma cruzi of the vector triatoma infestans fed on infected humans and dogs in northwest argentina.the probability that an uninfected triatoma infestans would become infected with trypanosoma cruzi after a single feed on people or dogs seropositive for t. cruzi was estimated in amama, a rural village in northwest argentina where transmission had resurged four years earlier. the prevalence of seropositivity for t. cruzi was 34.2% among 225 people tested, and 65.1% among 83 dogs tested. parasitemia was detected by xenodiagnosis in 29.3% of 41 seropositive persons and in 85.3% of 34 seropositive ...19968702018
[current human and canine seroprevalence of chagasic infection in san pedro de atacama county, ii region of antofagasta, chile, 1995].in order to assess the impact of a control program against triatoma infestans launched in 1988, based on insecticide spraying of dwellings, a serological survey for chagasic infection was carried out during 1995 in three localities from san pedro de atacama county (22 degrees 55' south lat., 68 degrees 12' west long.), ii region of antofagasta in northern chile. blood samples from 531 children and adolescents and 65 dogs were subjected to elisa test and indirect immunofluorescent test for chagas ...19969302779
[evaluation of vectors of chagas' disease eradication programs in chile by serological study of children under 10 years old].chagas' disease is a parasitic zoonosis with high prevalence in chile. it is distributed in rural and periurban section in the northern most seven out of thirteen regions in which the country is divided, and affects about 142,000 individuals dispersed in most of the 165 counties sited in the chagasic zone. triatoma infestans--intradomiciliary species--is the main and practically exclusive vector of trypanosoma cruzi. diverse tools may be utilized in order to interrupt the domestic cycle of trans ...19969302780
[chagas disease: impact of triatoma infestans control program in alto del carmen, huasco province, iii region atacama, chile].a control program of triatoma infestans has been carried out in alto del carmen, an endemic chagasic rural county in the iii region, chile. the program started in 1988 with an attack phase consisting in a masshouse spraying with residual insecticide, followed by an entomological surveillance phase with health education for community participation and vector detection in eventually reinfested houses. a yearly evaluation in 1992, 1993, 1994 and 1995 was carried out in order to determine the effect ...19969196951
four tdr diseases can be "eliminated". 3. chagas success in brazil and colombia.recent figures published by brazil's chagas disease control program indicate that the disease will soon be eradicated in brazil. for example, in 1982, 711 brazilian municipalities reported infestation with triatoma infestans, the most important domiciliary vector of chagas disease. however, in 1993, only 83 municipalities reported any infestation. disease transmission through blood transfusion in the country has also been dramatically reduced. in 1982, 6.5% of blood donations in brazil were ...199612294419
anticoagulant activity of triatoma infestans and panstrongylus megistus saliva (hemiptera/triatominae).salivary gland extract of triatoma infestans prolonged thrombin time, prothrombin time, and activated partial thromboplastin time, while salivary gland extract of panstrongylus megistus delayed thrombin time and activated partial thromboplastin time. however, saliva of p. megistus prolonged activated partial thromboplastin time and saliva of t. infestans delayed activated partial thromboplastin time and prothrombin time. t. infestans saliva interferes particularly with activity of factor viii (i ...19968790775
effect of phospholipids on the structure of triatoma infestans lipophorin studied by fluorescence methods.to study the role of phospholipids in the lipophorin structure, they were removed by phospholipase a2 treatment. fluorescence lifetimes and accessibility to acrylamide quenching of different diphenylhexatrienyl derivatives, which were used as analogues of the different lipid components, indicate a surface localization of phospholipids and a distribution of diacylglycerols between the core and the surface, the surface fraction being increased by the phospholipase a2 treatment. the rotational beha ...19968906590
detection and quantification of a very high density lipoprotein in different tissues of triatoma infestans during the last nymphal and adult stages.the presence of a very high density lipoprotein (vhdl), an hexameric protein, was explored in different tissues of triatoma infestans throughout the last nymphal and adult stages, and in egg extracts by western blot assays. the vhdl was always detected in both, hemolymph and fat body, during the above mentioned stages and it was also observed in the buffer soluble fraction of testis and egg homogenates. an enzyme-linked immunosorbent assay (elisa) was used to measure the vhdl titer in these tiss ...19968995792
polarized microscopy study of an antennal sensillum of triatoma infestans: an ordered distribution of chitin fibrils and associated components.aspects of the molecular organization of the antennal sensilla trichodea of triatoma infestans have been investigated with the use of both polarization and scanning electron microscopy. the sensilla have a smooth surface with minute bulbs on the tapered end. they showed strong positive birefringence, irrespective of the refractive index of the imbibing medium. the plotting of a form birefringence (fb) curve for native material showed slight increases in the retardation values with increases of t ...19968673617
meiotic behaviour of holocentric chromosomes: orientation and segregation of autosomes in triatoma infestans (heteroptera).the meiotic behaviour of the holocentric chromosomes of the heteropteran species triatoma infestans has been analysed by means of orcein staining and c-banding on squashed spermatocytes. we have focused our analysis on chromosome 3, which shows a large distal heterochromatic band at one of the ends of both homologues. at metaphase i, and independently of the chiasma position, two alternative orientations have been observed: either the heterochromatic or the euchromatic ends of both homologues ar ...19979088643
biochemistry of the evolution of triatoma infestans. xii. biosynthesis and secretion of a very high density lipoprotein.biosynthetic processes related to the production of an insect hexamerin, very high density lipoprotein (vhdl), have been examined in the fat body of fifth-instar nymph and adult triatoma infestans. fat bodies were incubated in vitro with [3h]leucine and the incubation media were precipitated using a specific antiserum. the sds-polyacrylamide gel electrophoresis followed by blotting on nitrocellulose showed that both larval and adult fat body secreted the vhdl subunit. moreover, the radiolabel re ...19979339237
two hemocyte populations in triatoma infestans: ultrastructural and lectin-binding characterization.two distinct hemocyte populations are determined in the hemolymph of the triatomine bug triatoma infestans klug, oenocytoids and plasmatocytes, and their independent origin from separate stem cells is shown. both hemocyte populations differ considerably in their morphology, ultrastructure and lectin-binding properties. while oenocytoids are quite uniform with easily definable cells which do not to bind any assayed lectin, the plasmatocytes are a very polymorphic population possessing several mor ...19979188175
chagas disease vector control in central america.as the southern cone initiative proceeds steadily towards eradication of triatoma infestans, there is increasing interest in applying similar approaches to control chagas disease vectors in mexico, central america and countries of the andean pact. here, chris schofield and jean-pierre dujardin discuss the technical feasibility of such an approach.199715275100
acid phosphatase activity in malpighian tubules of triatoma infestans klug.acid phosphatase activity was detected in the malpighian tubules of the blood-sucking hemipteran, triatoma infestans. the enzyme activity was especially prominent in the cytoplasmic globules which were assumed to be laminated 'concretions', which occur in the distal cells of the organ. it was also verified in the nuclei and in some cytoplasmic granules (lysosomes) of the proximal cells. the data indicated that lysosomes were involved with the nature or origin of the laminated concretions, but it ...19979682470
the use of morphometrics in entomological surveillance of sylvatic foci of triatoma infestans in bolivia.jamach'uma (cochabamba, bolivia) is a small village surrounded by sylvatic foci of triatoma infestans. houses in the village were also infested with t infestans, and were sprayed in december 1992 as part of a chagas disease vector control trial. ten months later the houses were found to be again infested with a few fifth instar nymphs of t. infestans. these nymphs were compared by seven head measurements with 36 fifth instar nymphs collected from houses in jamach'uma before treatment, and with t ...19979210965
entomopathogenous fungi degrade epicuticular hydrocarbons of triatoma infestans.studies were undertaken to analyze the ability of entomopathogenous fungi to degrade insect hydrocarbons. strains of beauveria bassiana and metarhizium anisopliae pathogenic to the blood-sucking bug triatoma infestans were grown on hydrocarbon and non-hydrocarbon insect lipid extracts and on synthetic hydrocarbon-enriched media as the sole carbon source. entomopathogenous fungi were shown to utilize hydrocarbons as the only carbon source for their growth. insect-derived hydrocarbons served more ...19979244399
[the finding of panstrongylus megistus in an artificial ecotope: taking up residence or merely visiting?].as a first measure of chagas' disease control in brazil with chemical elimination of the most important vector of the disease, triatoma infestans was removed. attention is now being paid to triatoma sordida and panstrongylus megistus. that species can eventually be found inside houses, as happened with the specimens we examined from bernardino de campos and sete barras, all of them infected by trypanosoma cruzi. these data suggest that a better knowledge about the behavior that species is needed ...19979265230
[evaluation of beta-cypermethrin for the control of triatoma infestans].this study assessed the insecticidal effect in the laboratory and in the field of the new pyrethroid beta-cypermethrin against triatoma infestans, the vector of chagaƛ disease, and compared it with that of deltamethrin. comparison of the 50% lethal dosis (ld50) of both pyrethroids showed that beta-cypermethrin is more effective against the nymphs and that deltamethrin is more effective against the adults. evaluation of the 50% lethal concentration (lc50) of the flowable formulations of both inse ...19979273197
molecular karyotype and schizodeme analyses of trypanosoma cruzi stocks from chilean triatomines.forty-one trypanosoma cruzi stocks isolated from the chilean vectors triatoma infestans and triatoma spinolai were characterized by pulse-field gel electrophoresis and southern blotting with the cruzipain gene, and by schizodeme analysis of kinetoplast dna with ecori and msp i. seven parasite groups were found by molecular karyotype which correlate with schizodeme and multilocus enzyme electrophoresis, supporting the concept of clonal propagation for trypanosoma cruzi. a predominant t. cruzi sto ...19979280894
chagas disease vector control in tupiza, southern bolivia.heavy domestic and peridomestic infestations of triatoma infestans were controlled in two villages in southern bolivia by the application of deltamethrin sc25 (2.5% suspension concentrate) at a target dose of 25 mg a.i./m2. actual applied dose was monitored by hplc analysis of filter papers placed at various heights on the house walls, and was shown to range from 0 to 59.6 about a mean of 28.5 mg a.i./m2. wall bioassays showed high mortality of t. infestans during the first month after the appli ...19979302405
attempts to improve xenodiagnosis: comparative test of sensibility using rhodnius neglectus, panstrongylus megistus, triatoma vitticeps and triatoma infestans in endemic areas of brazil.from june 1984 to july 1992, 392 xenodiagnostic tests were applied on 264 patients with chronic chagas disease from brazilian endemic areas of virgem da lapa and coronel murta, situated in the jequitinhonha valley, in the state of minas gerais. the susceptibilities of rhodnius neglectus, panstrongylus megistus, triatoma vitticeps and triatoma infestans were compared. most of the time 20 nymphs (fourth instar) of each species were applied to 161 women and 103 men aged between 5 and 83 years of ag ...19979302418
infection of triatoma guasayana, triatoma sordida and triatoma infestans by trypanosoma cruzi from a naturally infected opossum. 19979332580
biomorphological alterations induced by an anti-juvenile hormonal compound, 2-(2-ethoxyethoxy)ethyl furfuryl ether, on three species of triatominae larvae (hemiptera, reduviidae).applied topically to larvae of rhodnius prolixus stal, triatoma infestans (klug) and panstrongylus herreri wygodzinsky, vectors of trypanosoma cruzi, the causative agent of chagas' disease, a synthetic, furan-containing anti-juvenile hormonal compound, 2-(2-ethoxyethoxy)ethyl furfuryl ether induced a variety of biomorphological alterations, including precocious metamorphosis into small adultoids with adult abdominal cuticle, ocelli, as well as rudimentary adultoid wings. some adultoids died duri ...19979332589
characterization of trypanosoma cruzi strains isolated from chronic chagasic patients, triatomines and opossums naturally infected from the state of rio grande do sul, brazil.thirty-five trypanosoma cruzi strains were isolated from chronic chagasic patients, triatomines and opossums from different municipalities of the state of rio grande do sul. parasites were characterized by means of mice infectivity, enzyme electrophoresis and randomly amplified polymorphic dna (rapd) analysis. twenty-nine strains were isolated from chagasic patients, 4 from triatomines (2 from triatoma infestans and 2 from panstrongylus megistus) and 2 from opossums didelphis albiventris. thirty ...19979332599
genetic variability and microdistribution of triatoma infestans genotypes and trypanosoma cruzi clones in arequipa region (peru).the genetic variability of triatoma infestans and trypanosoma cruzi populations was studied by isoenzyme analysis in two distinct areas of arequipa province (peru); one, santa rita de siguas, being an endemic area for chagas' disease, the second, arequipa, recently infected. analysis of t. infestans genetic variability indicates, (i) temporal stability of genotypes found in santa rita de siguas, (ii) high genetic differences between arequipa and santa rita de siguas populations suggesting minor ...19979332609
incoordination, paralysis and recovery after pyrethroid treatment on nymphs iii of triatoma infestans (hemiptera: reduviidae).symptoms of poisoning for deltamethrin and cis-permethrin on nymphs iii of triatoma infestans were described. the time required for incoordination and paralysis were determined. deltamethrin was equal or more rapid in the onset of the first effect (accordingly to dose), and cis-permethrin in the onset of the second one. there were no significant differences between incoordination doses 50% (incd50s) at different times for the two pyrethroids. they showed equivalent incoordination power, but the ...19979332614
in vitro culture and phylogenetic analysis of "candidatus arsenophonus triatominarum," an intracellular bacterium from the triatomine bug, triatoma infestans.an intracellular symbiotic bacterium was isolated from the hemolymph of triatoma infestans and cultured in an aedes albopictus cell line. 16s ribosomal dna sequence analysis revealed that the bacterium was a member of the gamma-3 subgroup of the class proteobacteria, having 96.2% sequence identity with the most closely related bacterium, arsenophonus nasoniae, the causative agent of the son-killer trait in the parasitoid wasp nasonia vitripennis. these bacteria share morphological features and a ...19979336921
trypanosoma cruzi in the rectum of the bug triatoma infestans: effects of blood ingestion of the vector and artificial diuresis.the population density and the percentage of different developmental stages of an established infection of trypanosoma cruzi were determined at 40 days after the last feeding of the fourth instar in the rectum (lumen, anterior and posterior wall) of fed and unfed groups of fifth instars of triatoma infestans. additionally, the rectum and the malpighian tubules were incubated in saline, inducing diuresis by addition of the diuretic hormone. the rectum contained an average of 200,000-400,000 t. cr ...19979342743
metric differences between silvatic and domestic triatoma infestans (heteroptera: reduviidae) in bolivia.one hundred and fifty-seven specimens of bolivian triatoma infestans (klug 1834), including 44 from the silvatic focus at cochabamba (bolivia), were compared using morphometric characters of the head capsule. from these specimens, 10 silvatic and 28 domestic adults were also compared using additional morphometric characters of the membranous part of the hemelytra. nonparametric univariate comparisons between specimens from silvatic and domestic ecotopes indicated the importance of the postocular ...19979379460
human infection with trypanosoma cruzi in nasca, peru: a seroepidemiological survey.we estimated the proportion of seropositivity for infection with trypanosoma cruzi (chagas' disease) in a sample of the rural population of the province of nasca, department of ica, southwestern peru. although triatoma infestans, the only vector species identified in the department of ica, is often found in domestic environments, data of the extent of human infection with t. cruzi are scant. this study comprised 446 houses, known to be infested with triatomines, distributed in 19 rural localitie ...19979394524
trypanosoma cruzi: effect of immunization on the risk of vector-delivered infection in guinea pigs.the protective effect of experimental immunization was studied in guinea pigs exposed to vectorial infection by trypanosoma cruzi. immunized animals received an inoculum of live-attenuated t. cruzi epimastigotes into a granuloma previously induced by freund's complete adjuvant in the hind footpad. seven days later, a delayed-type hypersensitivity reaction was triggered by reinjection of the parasites in the front footpad. the animals were then placed in triatoma infestans-colonized corrals and e ...19979406779
effects of chickens on the prevalence of infestation and population density of triatoma infestans in rural houses of north-west argentina.effects of the presence of chickens on population density of the bug triatoma infestans, principal vector of chagas disease, were investigated by standardized sampling (indoors and peridomestically) from sixty-eight houses of three rural communities in north-west argentina, during march 1992. the domiciliary density of t. infestans increased linearly with the percentage of bugs that fed on chickens, as identified by agar double-diffusion tests. bug density was significantly higher in houses wher ...19979430119
[course of serologic positivity to chagas disease in a rural community in brazil].in order to assess the evolution of seropositivity for chagas' disease in the rural community of cássia dos coqueiros, in são paulo state, we compared two cross-sectional studies performed at that site in 1971-1972 and in 1989-1991. in the first survey the complement fixation test was used to determine seropositivity for the disease. in this study, the total prevalence of chagas' disease was found to be 16.6%, with values ranging from 2.9 to 61.9% (in the 10-19 and 50-59 year age groups, respect ...19979445769
detection of sylvatic dark morphs of triatoma infestans in the bolivian chaco. 19979566222
preliminary studies on temperature selection and activity cycles of triatoma infestans and t. spinolai (heteroptera:reduviidae), chilean vectors of chagas' disease.preferred temperature and activity patterns of triatoma infestans klug and triatoma spinolai porter were studied, both are vectors of chagas' disease in chile. in the laboratory, 24 t. spinolai and 18 t. infestans were exposed to a temperature gradient between 50 and 15 degrees c and a photoperiod of 14:10 (l:d) h. temperature and hourly position of bugs in an experimental chamber were recorded for 24 h. both species showed a cycle of preferred temperature, and both selected higher temperatures ...19979086704
spatial distribution of triatoma guasayana (hemiptera:reduviidae) in hardwood forest biotopes in santiago del estero, argentina.in the study area triatoma guasayana wygodzinsky & abalos is the only wild triatomine found sympatric with triatoma infestans (klug) in peridomestic premises. the trypanosoma cruzi chagas wild cycle is centered around the same biotopes occupied by t. guasayana, which are also visited mainly by opossums with annual prevalences of 29-50%. twelve hectares were sampled for 4 consecutive years during all seasons. during that time, 420 t. guasayana individuals were collected in 11.3% of 1,188 biotopes ...19979103752
the role of the peridomiciliary area in the elimination of triatoma infestans from rural argentine communities.the purpose of this study was to identify the origin of triatoma infestans reinfestation and study its dynamics following spraying with deltamethrin inside and around 94 houses in three rural communities in northwestern argentina. the effectiveness of the spraying was evaluated immediately after the houses were sprayed and two months later. in addition, five residual peridomiciliary foci were found and sprayed, as well as three preexisting ones that had not been sprayed. to monitor reinfestation ...19979149523
triatoma virus pathogenicity in laboratory colonies of triatoma infestans (hemiptera:reduviidae).in a survey of wild populations of triatoma infestans (klug) in argentina, 10% were infected with triatoma virus (trv). the virus also was detected in a laboratory colony 18 mo after being established, with infection rates up to 100%. mortality rate was 97.6% in nymphs and the molting process was inhibited, thereby increasing development time. because the virus was detected in colony nymphs. trv may be transmitted vertically. however, the higher infection rate in the colony compared with natural ...19979151486
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