Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| experimental infection of layer hens with a human isolate of brachyspira pilosicoli. | the anaerobic intestinal spirochaete brachyspira pilosicoli commonly colonizes the large intestine of a number of species, including chickens and human beings. the purpose of the current study was to determine whether an isolate of b. pilosicoli recovered from an hiv-infected patient with diarrhoea could infect and cause disease in adult chickens. over a 4-week period following experimental infection, a group of eight inoculated chickens showed a persistent and significant increase in faecal wat ... | 2003 | 12676876 |
| antimicrobial susceptibility testing of porcine brachyspira (serpulina) species isolates. | no standardized method for susceptibility testing of brachyspira spp. is currently available. a broth dilution procedure was evaluated and used to test the activities of six antimicrobial agents for 108 isolates of swedish porcine brachyspira spp. representing biochemical groups i, ii, and iii. group i corresponds to brachyspira hyodysenteriae, group ii corresponds to b. intermedia, and group iii corresponds to b. murdochii and b. innocens. a panel was designed with the antimicrobial agents drie ... | 2003 | 12791886 |
| in vitro antimicrobial susceptibility of brachyspira pilosicoli isolates from humans. | the in vitro antimicrobial susceptibility of the anaerobic intestinal spirochete brachyspira pilosicoli was investigated by an agar dilution method. human (n = 123) and porcine (n = 16) isolates were susceptible to metronidazole, ceftriaxone, meropenem, tetracycline, moxifloxacin, and chloramphenicol; erythromycin and ciprofloxacin were not active. resistance to amoxicillin and clindamycin varied. amoxicillin susceptibility was restored by clavulanic acid. | 2003 | 12821498 |
| characterization of a locus encoding four paralogous outer membrane lipoproteins of brachyspira hyodysenteriae. | the identification of brachyspira hyodysenteriae outer membrane proteins (omps) that may stimulate immunity to swine dysentery is important for vaccine development. we report here the analysis of a novel locus, blpgfea, encoding four tandem paralogous proteins of approximately 30 kda from b. hyodysenteriae. the four proteins share 31-39% sequence identity with lipoproteins from several species of bacterial pathogens, but the locus possesses a unique genetic organization. using antisera raised to ... | 2003 | 12706440 |
| development of a duplex pcr assay for detection of brachyspira hyodysenteriae and brachyspira pilosicoli in pig feces. | a duplex pcr (d-pcr) amplifying portions of the brachyspira hyodysenteriae nadh oxidase gene and the b. pilosicoli 16s rrna gene was developed and then tested on dna extracted from 178 porcine fecal samples. the feces also underwent anaerobic culture and species-specific pcrs. fecal extraction-d-pcr detected seven additional samples containing b. hyodysenteriae and five more containing b. pilosicoli. | 2003 | 12843096 |
| clinical coccidiosis in a boar stud. | this report describes an outbreak of coccidiosis in a boar stud. a live, untreated, adult boar with a history of diarrhea was submitted to the iowa state university veterinary diagnostic laboratory, ames, ia. for a 3-month period, approximately 40% of the boars in this stud had developed gray to brown diarrhea that lasted 1-3 days. affected boars did not lose condition, and antibiotic therapy did not appear to affect the clinical course ofthe disease. at necropsy, the distal ileum was palpably t ... | 2003 | 12918824 |
| detection by pcr and isolation assays of the anaerobic intestinal spirochete brachyspira aalborgi from the feces of captive nonhuman primates. | the purpose of this study was to investigate the presence of the anaerobic intestinal spirochetes brachyspira aalborgi and brachyspira pilosicoli in the feces of captive nonhuman primates (n = 35) from 19 species housed at the zoological gardens, perth, western australia. both spirochete species are known to infect human beings. dna was extracted from freshly collected feces with a commercially available qiaamp dna stool minikit and subjected to pcr protocols amplifying portions of the 16s rrna ... | 2003 | 12624050 |
| brachyspira hyodysenteriae contains eight linked gene copies related to an expressed 39-kda surface protein. | a tandemly linked set of four open reading frames (orfs), identified as vspa-d (variable surface protein) had been identified from previous cloning and sequencing of clones from a genomic library constructed from brachyspira hyodysenteriae strain b204. the predicted translation products of these closely related genes were homologous to (but not identical with) a characterized 39-kda surface-exposed membrane protein from this animal pathogen. additional screening of the genomic library has been p ... | 2003 | 12593966 |
| clinical significance of human intestinal spirochetosis--a morphologic approach. | intestinal spirochetosis (is) is a condition defined morphologically by the presence of spirochetal microorganisms attached to the apical cell membrane of the colonic and rectal epithelium. intestinal spirochetes comprise a heterogeneous group of bacteria. in humans brachyspira aalborgi and brachyspira pilosicoli predominate. prevalence rates of is are low where living standards are high, in contrast to poorly developed areas where is is common. homosexuals and hiv-infected individuals are at hi ... | 2003 | 14556061 |
| an outbreak of histomoniasis in free-range layer hens. | histomonas meleagridis was held primarily responsible for an outbreak of 6% increased mortality and 11% decreased egg production between weeks 57 and 72 in a flock of free-range layer hens, concurrently infected with brachyspira-like bacteria. this case can be considered an example of ancient diseases re-emerging in alternative housing systems that are promoted because of animal welfare considerations, but that at the same time allow rapid spread of pathogens in birds for which only few curative ... | 2003 | 12850921 |
| intestinal spirochaetes of the genus brachyspira: an update on recent findings. | 2003 | 14753198 | |
| detection of bacteriophage vsh-1 svp38 gene in brachyspira spirochetes. | vsh-1 is a mitomycin c-inducible, non-lytic, phage-like agent that packages random 7.5-kb fragments of the brachyspira hyodysenteriae genome. vsh-1 is the first recognized mechanism for gene transfer between b. hyodysenteriae cells. to analyze the distribution of vsh-1 among spirochetes, a 344-bp probe for gene svp38, encoding the vsh-1 major head protein, was amplified by polymerase chain reaction and used in southern blot hybridizations with genomic dna from various spirochete genera. the svp3 ... | 2003 | 12892886 |
| preparation and characterization of polyclonal and monoclonal antibodies against lawsonia intracellularis. | proliferative enteropathy is an intestinal infectious disease caused by the obligate intracellular bacterium lawsonia intracellularis. immunohistochemistry staining has superior sensitivity over hematoxylin and eosin and silver staining for detecting l. intracellularis in histological sections. a l. intracellularis-specific monoclonal antibody (mab) produced in the uk (ig4 mab) has been described in the literature. however, no monoclonal or polyclonal antibodies are commercially available. there ... | 2003 | 14535543 |
| differential requirements for proliferation of cd4+ and gammadelta+ t cells to spirochetal antigens. | alphabeta+ and gammadelta+ t cells have different mechanisms of epitope recognition and are stimulated by antigens of different chemical nature. an immunization model with antigens from the spirochete brachyspira hyodysenteriae was used to examine the requirements for proliferation of circulating porcine cd4+ and gammadelta+ t cells in mixed lymphocyte cultures. cd4+ t cells only responded to stimulation with b. hyodysenteriae antigens, whereas gammadelta+ t cells proliferated when cultures were ... | 2003 | 14572799 |
| colonic spirochetosis in children and adults. | we undertook a retrospective analysis of colonic spirochetosis in 14 cases: females, 3; males, 11; children, 4; adults, 10. two men had hiv infections. all children and both hiv-infected men had abdominal complaints, diarrhea, or both. most other adults underwent colonoscopy for polyp screening (n = 4) or follow-up of crohn disease (n = 1) or had other indications (n = 2) or diarrhea (n = 1). histologically, spirochetosis was identified in all parts of the colon and was not strongly associated w ... | 2003 | 14671970 |
| survival of intestinal spirochaete strains from chickens in the presence of disinfectants and in faeces held at different temperatures. | this study aimed to evaluate the efficacy of some commonly used disinfectants in inactivating the pathogenic avian intestinal spirochaetes brachyspira intermedia and brachyspira pilosicoli, and to examine spirochaete survival in chicken caecal faeces held at 4 degrees c, 25 degrees c or 37 degrees c. six disinfectants were evaluated at their recommended working concentrations: alkaline salts, quaternary ammonium, iodine as an iodophor, chlorine from a chlorine-release agent, glutaraldehyde and h ... | 2003 | 14676016 |
| evaluation of selective media for the isolation of brachyspira aalborgi from human faeces. | the purposes of this study were to identify a solid medium that supports improved growth of the anaerobic intestinal spirochaete brachyspira aalborgi, to modify this for use as a selective isolation medium and then to test the medium for its effectiveness in isolating b. aalborgi from patients' faeces. of the media evaluated, brain heart infusion agar (bhia) with 10 % bovine blood (bb) was the most effective base-supplement combination for growth, with colonies attaining 1.2 mm in diameter by 21 ... | 2003 | 12748271 |
| colonization and risk factors for brachyspira aalborgi and brachyspira pilosicoli in humans and dogs on tea estates in assam, india. | the prevalence of colonization with the anaerobic intestinal spirochaetes brachyspira aalborgi and brachyspira pilosicoli was investigated in humans (n = 316) and dogs (n = 101) living on three tea estates in assam, india. colonization was detected using pcr on dna from faeces. nineteen (6%) human faecal samples contained b. aalborgi dna, 80 (25.3%) contained b. pilosicoli dna, and 10 (3.2%) contained dna from both species. one canine sample contained dna from b. pilosicoli. significant factors ... | 2004 | 14979599 |
| [a case of intestinal spirochaetosis due to brachyspira aalbolgi]. | 2004 | 15011440 | |
| brachyspira research - special issue on colonic spirochaetes of medical and veterinary significance. | 2004 | 15017280 | |
| differences in lymphocyte subpopulations and cell counts before and after experimentally induced swine dysentery. | the aim of this study was to examine the levels of circulating leukocytes and lymphocyte subpopulations before and immediately after experimentally induced swine dysentery. twenty-one healthy crossbred pigs (approximately 22 kg) were orally inoculated with brachyspira hyodysenteriae. blood was sampled before inoculation and when clinical signs of swine dysentery occurred. pigs that remained healthy were sampled when killed. total and differential white blood cell counts were performed, and lymph ... | 2004 | 15017281 |
| experimental swine dysentery: comparison between infection models. | the aim of the present study was to develop a reproducible porcine infection model with brachyspira hyodysenteriae. the influence of different factors was evaluated, namely, age, a diet containing large quantities of soybean meal, housing and administration of cortisol or antacids. furthermore, the synergistic effect of additional bacteria (escherichia coli o141, bacteroides vulgatus or a mixture of bacteroides fragilis, a field isolate of bacteroides and fusobacterium necrophorum) was studied. ... | 2004 | 15017282 |
| analysis of genetic variation in brachyspira aalborgi and related spirochaetes determined by partial sequencing of the 16s rrna and nadh oxidase genes. | the purpose of this study was to investigate genetic variation in the anaerobic intestinal spirochaete brachyspira aalborgi by partial sequencing of the 16s rrna and nadh oxidase genes. the spirochaete is poorly cultivable; hence, only six isolates were available for analysis. additional sequences were amplified from dna extracted from fixed colorectal biopsies from 26 patients with histological evidence of intestinal spirochaetosis, and from the faeces of six non-human primates (nhp). multiple ... | 2004 | 15017291 |
| demonstration of brachyspira aalborgi lineages 2 and 3 in human colonic biopsies with intestinal spirochaetosis by specific fluorescent in situ hybridization. | sequences of known 16s rrna genes, derived from sequence analysis of cloned 16s rdna, were used to design a specific oligonucleotide probe targeting spirochaetes of brachyspira aalborgi lineages 2 and 3. the probe was used with fluorescent in situ hybridization to study the involvement of these organisms in human intestinal spirochaetosis. seventeen human colonic biopsies from norway and denmark with intestinal spirochaetosis caused by brachyspira-like organisms different from the type strain of ... | 2004 | 15017292 |
| efficacy of seven disinfectant sanitisers on field isolates of brachyspira pilosicoli. | 2004 | 15119732 | |
| [colonic spirochetes: an infrequent cause of adult diarrhea]. | large intestine infection due to spirochetes was described in 1967 and is mainly related to two pathogens, serpulina pilosicoli and brachyspira aalborgi. clinically, infection presents as diarrhea and/or rectorrhagia and is more frequent among homosexuals. its prevalence is difficult to estimate but significant differences have been described according to the socioeconomic level of the area studied. we describe three cases of diarrhea due to spirochetes, which are of interest due to the lack of ... | 2004 | 14718105 |
| further characterization of porcine brachyspira hyodysenteriae isolates with decreased susceptibility to tiamulin. | brachyspira hyodysenteriae is the causative agent of swine dysentery, a severe diarrhoeal disease in pigs. few drugs are available to treat the disease, owing to both antimicrobial resistance and withdrawal of drugs authorized for use in pigs. tiamulin is the drug of choice in many countries, but isolates with decreased susceptibility have recently been reported. the mechanism of tiamulin resistance in b. hyodysenteriae is not known and this facet is essential to understand the dissemination of ... | 2004 | 15017283 |
| molecular and ultrastructural characterization of porcine hippurate-negative brachyspira pilosicoli. | brachyspira pilosicoli, the causative agent of porcine intestinal spirochetosis, usually has hippurate-cleaving capacity. we have regularly isolated hippurate-negative b. pilosicoli from cases of porcine diarrhea. in this study, we show that these biochemically atypical b. pilosicoli isolates can be classified as b. pilosicoli. 16s ribosomal dna was partially sequenced from eight hippurate-negative and two hippurate-positive b. pilosicoli-like isolates from seven herds. the differences in nucleo ... | 2004 | 15243075 |
| decreased susceptibility to tiamulin and valnemulin among czech isolates of brachyspira hyodysenteriae. | the agar dilution method was used to investigate the sensitivity to pleuromutilins of 100 isolates of brachyspira hyodysenteriae isolated from 63 pig farms between 1997 and 2001. in the period under investigation, mics to both tiamulin and valnemulin increased, with differences between the periods 1997-98 and 1999-2001 being statistically significant (p < 0.001 for tiamulin and p < 0.0001 for valnemulin). between 1997 and 2001, the mic50 and mic90 of tiamulin increased from 0.062 and 0.25 microg ... | 2004 | 15017284 |
| genetic variation in brachyspira: chromosomal rearrangements and sequence drift distinguish b. pilosicoli from b. hyodysenteriae. | brachyspira pilosicoli and b. hyodysenteriae are anaerobic pathogenic intestinal spirochetes differing in host range and disease manifestations. little is known about the size, organization, or genetic content of the b. pilosicoli genome and only limited information is available regarding the genetic organization in b. hyodysenteriae. both b. hyodysenteriae and b. pilosicoli exist as recombinant populations, and this may be due, in part, to an unusual phage-like gene transfer agent, vsh-1. to co ... | 2004 | 16701522 |
| brachyspira hyodysenteriae and other strongly beta-haemolytic and indole-positive spirochaetes isolated from mallards (anas platyrhynchos). | the aims of the current study were to collect intestinal spirochaetes (genus brachyspira) from farmed and wild mallards (anas platyrhynchos) and to identify and classify those isolates that phenotypically resembled brachyspira hyodysenteriae, an enteric pathogen of pigs. the isolation rate of brachyspira spp. was high from both farmed (93 %) and wild mallards (78 %). in wild mallards, it appeared that brachyspira spp. were more likely to be found in migratory birds (multivariate analysis: rr = 1 ... | 2004 | 15017285 |
| immunomagnetic separation of the intestinal spirochaetes brachyspira pilosicoli and brachyspira hyodysenteriae from porcine faeces. | porcine intestinal spirochaetes are fastidious anaerobic organisms and, as a consequence, it has been necessary to develop various protocols to enhance their isolation from or detection in faeces. immunomagnetic separation (ims) is a method developed recently to improve separation of target cells from mixed cell suspensions. the purpose of the present study was to compare the relative sensitivity of ims for isolation of brachyspira pilosicoli and brachyspira hyodysenteriae with current routine d ... | 2004 | 15017286 |
| identification of three clusters of canine intestinal spirochaetes by biochemical and 16s rdna sequence analysis. | it has been suggested that canine intestinal spirochaetes consist of brachyspira pilosicoli and a group of strains that has been provisionally designated 'brachyspira canis'. the purpose of the present study was to compare 22 spirochaete isolates that were obtained from intestinal specimens of dogs in sweden (n = 12), norway (n = 4), the united states (n = 3), australia (n = 2) and germany (n = 1) with type and reference strains, as well as field isolates, of brachyspira species by five biochemi ... | 2004 | 15017293 |
| postweaning multisystemic wasting syndrome--pmws. the first year with the disease in sweden. | postweaning multisystemic wasting syndrome (pmws) is causally associated with porcine circovirus type 2 (pcv2) infection of pigs. pcv2 has been present in the swedish pig population for at least ten years. despite this, no signs of pmws were observed in pigs of sweden until 2003. since then the disease has spread slowly in sweden. the pig population of sweden is geographically isolated, the density of pigs and the pathogen load in the country is low and the trade of animals is organised in a res ... | 2004 | 15663213 |
| a cross-sectional study to investigate the occurrence and distribution of intestinal spirochaetes (brachyspira spp.) in three flocks of laying hens. | a cross-sectional study was conducted on a commercial egg-producing farm with a history of wet litter. a total of 600 fresh caecal faecal samples were obtained from under cages of laying hens in three sheds each containing flocks of approximately 5400 hens. samples were cultured for intestinal spirochaetes, and growth on the primary isolation plate was observed under a phase contrast microscope and subjected to pcrs specific for the intestinal spirochaetes brachyspira intermedia and brachyspira ... | 2004 | 15708815 |
| extensive intestinal spirochaetosis in pigs challenged with brachyspira pilosicoli. | a field isolate of brachyspira pilosicoli, the aetiological agent of porcine intestinal spirochaetosis, was inoculated by stomach tube into six 6-week-old pigs. all animals developed loose to watery faeces and were killed successively on days 8, 14 or 17 post-inoculation (day 17 being the end of the study). mild mucosal reddening and flecks of pus characterized the gross lesions, while diffuse, catarrhal colitis was revealed microscopically in all animals. intestinal spirochaetosis with moderate ... | 2004 | 15017287 |
| prevalence, risk factors and molecular epidemiology of brachyspira pilosicoli in humans on the island of bali, indonesia. | the purpose of this study was to investigate the prevalence and epidemiology of the anaerobic intestinal spirochaete brachyspira pilosicoli amongst indonesians living in rural and urban settings on the island of bali. faecal samples (n = 992) were collected on two occasions, 4 months apart, from people living in four traditional farming villages, one peri-urban location and one urban area. samples were cultured anaerobically on selective agar and intestinal spirochaete isolates were confirmed to ... | 2004 | 15017290 |
| protection of pigs from swine dysentery by vaccination with recombinant bmpb, a 29.7 kda outer-membrane lipoprotein of brachyspira hyodysenteriae. | swine dysentery (sd) is an important endemic infection in many piggeries, and control can be problematic. in this study the efficacy of bmpb, a 29.7 kda outer-membrane lipoprotein of brachyspira hyodysenteriae, was evaluated as an sd vaccine. non-lipidated bmpb was expressed in escherichia coli as a histidine-tagged protein (his6-bmpb), or as an 8 kda carboxy-terminal portion fused to maltose-binding protein (mbp-bmpb-f604). the purified proteins were emulsified with oil-based adjuvants for intr ... | 2004 | 15288932 |
| brachyspira pilosicoli colonization in experimentally infected mice can be facilitated by dietary manipulation. | the purpose of this study was to determine whether defined dietary manipulations would enhance colonization of mice experimentally challenged with the intestinal spirochaete brachyspira pilosicoli. weanling c3h/hej mice (n = 48) were fed either a standard balanced mouse diet or a diet supplemented with 50 p.p.m. zinc bacitracin (znb), with 50 % (w/w) lactose or with both supplements. eight mice from each group were challenged orally with a human strain of b. pilosicoli (wesb), whilst four in eac ... | 2004 | 15017288 |
| biochemical properties of membrane-associated proteases of brachyspira pilosicoli isolated from humans with intestinal disorders. | a membrane-associated, subtilisin-like, serine protease activity was found in both pathogenic and non-pathogenic strains of brachyspira species in a previous study, but the biochemical properties of the enzyme were not investigated. the purpose of the present study was to characterize further the biochemical properties, including substrate specificity, of the membrane-associated protease of brachyspira pilosicoli isolated from humans with intestinal disorders. protease activity of detergent-enri ... | 2004 | 15017289 |
| influence of diet on the experimental infection of pigs with brachyspira pilosicoli. | the effects of five different diets on the experimental infection of pigs with a danish field isolate of brachyspira pilosicoli were investigated. the diets tested were a pelleted and a non-pelleted standard diet based on wheat and barley, the standard diet supplemented with 2 per cent lactic acid, a fermented liquid feed and a diet based on cooked rice. two trials were conducted, each with six groups of six pigs; in each, two of the groups were fed the standard diet. one of these groups and the ... | 2004 | 15029965 |
| the effect of antimicrobial growth promoter withdrawal on the health of weaned pigs in finland. | the use of the antimicrobial growth promoters (agps) carbadox and olaquindox has been banned in the european union (eu) since september 1999. we studied the effects of the withdrawal on the health of weaned piglets on two types of piglet-producing farms (farrowing herds and farrow-to-finish herds) from the different regions of finland. farms with no major problems with post-weaning diarrhoea were selected for the study to better evaluate the effect of agps alone. data on production, medication a ... | 2004 | 15579342 |
| identification and cloning of the gene encoding bmpc: an outer-membrane lipoprotein associated with brachyspira pilosicoli membrane vesicles. | the intestinal spirochaete brachyspira pilosicoli causes colitis in a wide variety of host species. little is known about the structure or protein constituents of the b. pilosicoli outer membrane (om). to identify surface-exposed proteins in this species, membrane vesicles were isolated from b. pilosicoli strain 95-1000 cells by osmotic lysis in dh(2)o followed by isopycnic centrifugation in sucrose density gradients. the membrane vesicles were separated into a high-density fraction (hdmv; rho=1 ... | 2004 | 15073313 |
| human intestinal spirochaetosis: any clinical significance? | spirochaetes are well known causative agents of diarrhoea in veterinary medicine. in human medicine the relationship between presence of spirochaetes in the colon on the one hand, and its clinical significance on the other, is far less clear. in the majority of cases the colonization of the colon with these micro-organisms seems to represent a commensal relationship with the host, and is almost always a coincidental finding with no association with the clinical symptoms of the patient whatsoever ... | 2004 | 15095857 |
| the wheat variety used in the diet of laying hens influences colonization with the intestinal spirochaete brachyspira intermedia. | this study investigated whether feeding different wheat varieties to laying hens could influence colonization with the intestinal spirochaete brachyspira intermedia. fifty isa-brown laying hens were divided into two groups. one group were fed a laying-hen diet formulated with wheat variety westonia, and one were fed the diet incorporating variety stilleto. each group was divided into 15 hens experimentally infected with b. intermedia and 10 uninfected controls. the 30 infected hens were housed i ... | 2004 | 15763727 |
| in vitro susceptibility of 21 antimicrobial agents to 37 isolates of brachyspira hyodysenteriae isolated from pigs in okinawa prefecture. | the in vitro susceptibilities to 21 antimicrobial agents, of 37 isolates of brachyspira (b.) hyodysenteriae isolated from pigs in okinawa meat center and a pig farm in okinawa prefecture, japan, were determined by the agar dilution method. carbadox was the most active of all the agents tested against the isolates (mic: <0.003 to 0.05). all the isolates were highly susceptible to olaquindox, tiamulin, dimetridazole, efrotomycin and valnemulin with mics ranging from </=0.1 to 1.6 microg/ml. penici ... | 2004 | 15107563 |
| [human intestinal spirochetosis]. | a characteristic feature of human intestinal spirochetosis (is) is the colonization of the mucosa of the large intestine with intestinal spirochetes of the genus brachyspira. the joining of the brachyspirae with the apical cellular membrane of enterocytes resembles in histological slides a false brush border of the intestinal mucosa. various symptoms related to the involvement of the large gut were found with invasive is. from the cultures of these cases were isolated brachyspira aalborgii and b ... | 2004 | 15146383 |
| the decrease in flaa observed in a flab mutant of borrelia burgdorferi occurs posttranscriptionally. | the lyme disease bacterium borrelia burgdorferi is a motile spirochete with a flat-wave morphology. the periplasmic flagella, which are situated between the outer membrane sheath and cell cylinder, are essential for both the cell's wavy shape and motility. here we focus on the structure and regulation of its periplasmic flagella. previous studies have suggested that the periplasmic flagella consist of a polymer of the major filament protein flab and a minor protein, flaa. we used immunoprecipita ... | 2004 | 15175283 |
| brachyspira pilosicoli isolated from two beagles and one mongrel in japan. | the feces, colonic contents, and colonic mucosa from two beagles and a mongrel with and without mucohemorrhagic diarrhea were anaerobically cultured and after 72 hr, lucent and rough colonies with weak beta-hemolysis were observed. small spirochetes with one or two loose waves in the colonies were observed under a phase contrast microscope and the spirochete cells sometimes aggregated. the 16srdna sequencing results demonstrated that the canine isolates were b. pilosicoli and the base alignments ... | 2004 | 15187377 |
| a possibility of application of the 105-kilodaltons protein of brachyspira alvinipulli cross-reacting with antisera to five species in the genus brachyspira to diagnosis. | the antigenic properties of brachyspira (b.) alvinipulli atcc 51933 and strain c2 were analyzed and compared with those of b. hyodysenteriae atcc 27164 and atcc 31212, b. pilosicoli atcc 51139, b. innocens atcc 29796 and b. aalborgi nctc 11492. in gel immunodiffusion tests, a protein in b. alvinipulli atcc 51933 reacted strongly with anti-b. alvinipulli atcc 51933-serum and formed two precipitin lines. furthermore, by an immunoblotting technique, the 105-kilodaltons (kda) protein in b. alvinipul ... | 2004 | 15297747 |
| brachyspira pilosicoli isolated from pigs in japan. | two of four weak beta-hemolytic isolates of intestinal spirochetes isolated from pigs in japan possessed a unique base alignment of tttttt on the 16s ribosomal dna of brachyspira pilosicoli and were identified as b. pilosicoli. the other two isolates were not identified by this technique. the identified isolates were 4.2 to 11 microm in length and 0.2 to 0.3 microm in diameter, 4 periplasmic flagella at each end were observed dominantly. the isolates were hippurate positive but indole negative. ... | 2004 | 15297763 |
| a wheat-based diet enhances colonization with the intestinal spirochaete brachyspira intermedia in experimentally infected laying hens. | this study investigated the influence of feeding diets based on cereals with different non-starch polysaccharide content on colonization with the intestinal spirochaete brachyspira intermedia in experimentally infected laying hens. the diets were based on wheat, barley, or barley and sorghum, all with or without supplementation with exogenous dietary enzymes. six groups of 12 individually caged laying hens were fed the respective diets for 2 weeks, and then challenged at 20 weeks of age by crop ... | 2004 | 15370044 |
| antimicrobial resistance in brachyspira pilosicoli with special reference to point mutations in the 23s rrna gene associated with macrolide and lincosamide resistance. | a point mutation in the 23s rrna gene causes macrolide and lincosamide resistance in brachyspira hyodysenteriae. the possible occurrence of a similar mutation in brachyspira pilosicoli was studied and the mics of six antimicrobial agents for swedish field isolates of b. pilosicoli were determined. of 10 isolates with high mics of macrolide and lincosamide antibiotics, six had a mutation in nucleotide position 2058 or 2059 in the 23s rrna gene as compared to the wild type of escherichia coli, whe ... | 2004 | 15383163 |
| occurrence of lawsonia intracellularis and brachyspira spp. infection in swine suffering from diarrhoea. | principal aim of this study was to examine fecal samples from pigs suffering from diarrhea for the presence of lawsonia intracellularis, brachyspira hyodysenteriae and brachyspira pilosicoli. the molecular techniques such as pcr and nested pcr were employed to detect the presence of p78 fragment of genomic dna specific for lawsonia intracellularis as well as fragment of tlya gene specific for brachyspira hyodysenteriae and 16s rdna gene of brachyspira pilosicoli. we assumed that about 25% of pig ... | 2004 | 15478867 |
| comparison of methods for antimicrobial susceptibility testing and mic values for pleuromutilin drugs for brachyspira hyodysenteriae isolated in germany. | in germany treatment of swine dysentery is hampered by brachyspira hyodysenteriae strains showing elevated mic values to the few antibiotics licensed. therefore, susceptibility testing of clinical isolates is an important service to the swine practitioner. this study compares the established agar dilution procedure for antimicrobial susceptibility testing of this fastidious anaerobe to the broth microdilution test newly developed [anim. health res. 2 (2001) 59; vet. microbiol. 84 (2002) 123; j. ... | 2004 | 15288924 |
| mutations in ribosomal protein l3 and 23s ribosomal rna at the peptidyl transferase centre are associated with reduced susceptibility to tiamulin in brachyspira spp. isolates. | the pleuromutilin antibiotic tiamulin binds to the ribosomal peptidyl transferase centre. three groups of brachyspira spp. isolates with reduced tiamulin susceptibility were analysed to define resistance mechanisms to the drug. mutations were identified in genes encoding ribosomal protein l3 and 23s rrna at positions proximal to the peptidyl transferase centre. in two groups of laboratory-selected mutants, mutations were found at nucleotide positions 2032, 2055, 2447, 2499, 2504 and 2572 of 23s ... | 2004 | 15554969 |
| [distribution of brachyspira hyodysenteriae and lawsonia intracellularis in healthy and diarrhoeic pigs]. | although brachyspira (b.) hyodysenteriae and lawsonia (l.) intracellularis are widely distributed in pigs in germany, there exists limited information on their clinical relevance. to get more insight into their potential role in swine diarrhoeal disease, in 2002 and 2003 faecal specimens from healthy pigs (n=1445) as well as from diarrhoeic pigs (n=2002) were tested by polymerase chain reaction (pcr) for the presence of both agents. of the specimens from healthy pigs l. intracellularis and b. hy ... | 2004 | 15584431 |
| rapid isolation of brachyspira hyodysenteriae and brachyspira pilosicoli from pigs. | the aim of this study was to compare and evaluate the time required to isolate brachyspira hyodysenteriae and brachyspira pilosicoli from porcine faeces. this was done using previously described selective media (spectinomycin) s400, (colistin, vancomycin and spectinomycin) cvs and (spectinomycin, vancomycin, colistin, spiramycin and rifampin with swine faecal extract) bj, compared with the method based on blood agar modified medium, with spectinomycin and rifampin (bam-sr), including a pre-treat ... | 2004 | 15708820 |
| neither hippurate-negative brachyspira pilosicoli nor brachyspira pilosicoli type strain caused diarrhoea in early-weaned pigs by experimental infection. | a hippurate-negative biovariant of brachyspira pilosicoli (b. pilosicolihipp-) is occasionally isolated in diarrhoeic pigs in finland, often concomitantly with hippurate-positive b. pilosicoli or lawsonia intracellularis. we studied pathogenicity of b. pilosicolihipp- with special attention paid to avoiding co-infection with other enteric pathogens. pigs were weaned and moved to barrier facilities at the age of 11 days. at 46 days, 8 pigs were inoculated with b. pilosicolihipp- strain br1622, 8 ... | 2005 | 16398337 |
| cecal spirochetosis caused by brachyspira pilosicoli in commercial turkeys. | spirochetes that were identified as brachyspira pilosicoli were present in the ceca of 7.5- to 18-wk-old turkeys with cecal spirochetosis and typhlitis. the identity of b. pilosicoli was confirmed on the basis of ultrastructural morphology of the cecal epithelium adherent microbes, immunohistochemical staining with a brachyspira genus-specific monoclonal antibody, and amplification of a b. pilosicoli species-specific 16s ribosomal rna (rrs gene) sequence by using the polymerase chain reaction an ... | 2005 | 16405009 |
| penicillin-binding proteins in the pathogenic intestinal spirochete brachyspira pilosicoli. | penicillin-binding proteins (pbps) of slightly different molecular masses (94, 62 or 68, 42 or 50, 25, and 22 kda) were identified in one human and two porcine brachyspira pilosicoli strains. identification of pbps of b. pilosicoli provides a basis for characterization of the genes encoding these proteins among pathogenic intestinal spirochetes of humans and animals. | 2005 | 15793139 |
| the use of multilocus enzyme electrophoresis to characterise intestinal spirochaetes (brachyspira spp.) colonising hens in commercial flocks. | multilocus enzyme electrophoresis (mlee) was used to identify, examine genetic relationships and look at disease associations of a collection of 53 intestinal spirochaete isolates previously recovered from the faeces of adult hens on 14 farms in qld, australia. the mlee results were compared with those previously obtained using species-specific pcr amplifications. the isolates were divided into five brachyspira species groups by mlee: brachyspira murdochii (n=17), b. intermedia (n=15), b. pilosi ... | 2005 | 15795087 |
| cd4+ t-cell responses and distribution at the colonic mucosa during brachyspira hyodysenteriae-induced colitis in pigs. | the spirochaete brachyspira hyodysenteriae causes swine dysentery, a severe colitis characterized by mucosal enlargement as a result of crypt elongation and epithelial necrosis. most efforts to understand the pathogenesis of this disease have focused on the aetiological agent and its virulence factors. however, the host immune response has been considered an important factor in disease development. previous research has shown that b. hyodysenteriae induces systemic cd4(+) and gammadelta t-cell r ... | 2005 | 15819705 |
| immunohistochemical and ultrastructural detection of intestinal spirochetes in thoroughbred horses. | studies of equine intestinal spirochetes have long focused on intestinal contents alone, but intestinal spirochetosis has been reported recently in a 21-month-old thoroughbred colt in japan. to define the clinical and pathological significances of intestinal spirochetosis in several horses, an epizootiologic survey with histologic, immunohistochemical, and ultrastructural methods was conducted for brachyspira antigen-containing intestinal spirochetes in 12 diseased or injured thoroughbred horses ... | 2005 | 15825495 |
| [the use of in-situ hybridization for the detection of brachyspira spp. in pigs]. | diagnosis of brachyspira infections in swine and the differentiation of the involved bacteria is time-consuming and in most cases unsatisfactory. detecting brachyspira directly in the damaged brachyspira of the large intestine could provide a direct correlation between histological lesionsa and bacterial growth. in this study we investigated whether in-situ hybridization (ish) with a digoxigenin-labeled rna-probe is a suitable method for detecting brachyspira in the mucosa of the large intestine ... | 2005 | 15900675 |
| the brachyspira hyodysenteriae ftna gene: dna vaccination and real-time pcr quantification of bacteria in a mouse model of disease. | the nucleotide sequence of the brachyspira hyodysenteriae ftna gene, encoding a putative ferritin protein (ftna), was determined. analysis of the sequence predicted that this gene encoded a protein of 180 amino acids. rt-pcr and western blot showed that the ftna gene was expressed in b. hyodysenteriae, and evidence suggests that ftna stores iron rather than haem. ftna was delivered as dna and recombinant protein vaccines in a mouse model of b. hyodysenteriae infection. vaccine efficacy was monit ... | 2005 | 15968507 |
| [severe diarrhoea with invasive intestinal spirochaetosis]. | a 38-year-old heterosexual male presented with a 6 to 7 week history of severe diarrhoea but no other complaints. in routine faecal cultures and routine parasitological investigations no pathogenic micro-organisms were observed. colonoscopy revealed a normal mucosa. multiple biopsies were obtained. the histological diagnosis was invasive intestinal spirochaetosis. the immunostain for borrelia burgdorferi cross-reacted with the spirochetes of the intestinal spirochaetosis. after two weeks of incu ... | 2005 | 16398171 |
| epidemiologic evaluation of diarrhea in dogs in an animal shelter. | to determine associations among infectious pathogens and diarrheal disease in dogs in an animal shelter and demonstrate the use of geographic information systems (giss) for tracking spatial distributions of diarrheal disease within shelters. | 2005 | 16008226 |
| colonic spirochetosis in animals and humans. | colonic spirochetosis is a disease caused by the gram-negative bacteria brachyspira aalborgi and brachyspira pilosicoli. b. pilosicoli induces disease in both humans and animals, whereas b. aalborgi affects only humans and higher primates. symptoms in humans include diarrhea, rectal bleeding, and abdominal cramps. colonic spirochetosis is common in third world countries; however, in developed countries, the disease is observed mainly in homosexual males. terminally ill patients infected with bra ... | 2005 | 16013401 |
| physiological achilles' heels of enteropathogenic bacteria in livestock. | an elaborate feeding regimen of animals, which takes advantage of the achilles' heels of enteropathogenic bacteria, can possibly enable prophylaxis in the intestinal tract, attenuate actual disease symptoms, accelerate recovery from a bacterial gastroenteritis or ensure food safety. there is a wide spectrum of conceivable weak spots in bacteria. some pathogenic bacteria cannot use certain compounds, or use them less efficient than beneficial bacteria. by addition of such substances to animal fee ... | 2005 | 16107037 |
| identification of genes of vsh-1, a prophage-like gene transfer agent of brachyspira hyodysenteriae. | vsh-1 is a mitomycin c-inducible prophage of the anaerobic spirochete brachyspira hyodysenteriae. purified vsh-1 virions are noninfectious, contain random 7.5-kb fragments of the bacterial genome, and mediate generalized transduction of b. hyodysenteriae cells. in order to identify and sequence genes of this novel gene transfer agent (gta), proteins associated either with vsh-1 capsids or with tails were purified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the n-terminal amino ... | 2005 | 16109929 |
| simultaneous detection of lawsonia intracellularis, brachyspira hyodysenteriae and salmonella spp. in swine intestinal specimens by multiplex polymerase chain reaction. | a multiplex pcr assay was developed for the simultaneous detection of the etiologic agents associated with porcine proliferative enteropathies (ppe), swine dysentery (sd) and porcine salmonellosis (ps) in a single reaction using dna from swine intestinal samples. single and multiplex pcr amplification of dna from lawsonia intracellularis, salmonella typhimurium and brachyspira hyodysenteriae with each primer set produced fragments of the predicted size without any nonspecific amplification, 210- ... | 2005 | 16131827 |
| amplification and sequencing of brachyspira spp. specific portions of nox using paraffin-embedded tissue samples from clinical colitis in austrian pigs shows frequent solitary presence of brachyspiramurdochii. | brachyspira infections are significant causes of enterocolitis in pigs. in order to differentiate pathogenic species (brachyspira (br.) hyodysenteriae, brachyspira pilosicoli) from less pathogenic or non-pathogenic species (brachyspira intermedia, brachyspira innocens, brachyspira murdochii) in paraffin-embedded tissue samples a polymerase chain reaction (pcr) protocol allowing identification of brachyspira at species level in archival material was developed. this approach was complemented by se ... | 2005 | 16213113 |
| the prevalences of brachyspira spp. and lawsonia intracellularis in swedish piglet producing herds and wild boar population. | the aim of the present study was to survey the prevalences of the enteric pathogens brachyspira hyodysenteriae, brachyspira pilosicoli and lawsonia intracellularis in swedish growing pigs and in the swedish wild boar population and to relate these findings to clinical signs. the study included 105 randomly selected herds, constituting approximately one third of swedish herds with a herd size of >100 sows. the herds were located all over the country. in these herds, growth promoters were not used ... | 2005 | 16283917 |
| laser capture microdissection of bacterial cells targeted by fluorescence in situ hybridization. | direct cultivation-independent sequence retrieval of unidentified bacteria from histological tissue sections has been limited by the difficulty of selectively isolating specific bacteria from a complex environment. here, a new dna isolation approach is presented for prokaryotic cells. by this method, a potentially pathogenic strain of the genus brachyspira from formalin-fixed human colonic biopsies were visualized by fluorescence in situ hybridization (fish) with a 16s rrna-targeting oligonucleo ... | 2005 | 16382905 |
| application of nox-restriction fragment length polymorphism for the differentiation of brachyspira intestinal spirochetes isolated from pigs and poultry in australia. | sixty-nine intestinal spirochetes isolated from pigs and poultry in eastern australia were selected to evaluate the effectiveness of a species-specific pcr-based restriction fragment length polymorphism (rflp) analysis of the brachyspira nox gene. for comparative purposes, all isolates were subjected to species-specific pcrs for the pathogenic species brachyspira hyodysenteriae and brachyspira pilosicoli, and selected isolates were examined further by sequence analysis of the nox and 16s ribosom ... | 2005 | 15825489 |
| assessment of diagnostics and antimicrobial susceptibility testing of brachyspira species using a ring test. | there is no ring test for quality assessment available in europe for diagnostics and antimicrobial susceptibility testing of the fastidious, anaerobic bacteria of the genus brachyspira. therefore, an international ring test for brachyspira spp. was performed once a year during 2002-2004. two sets of coded samples were prepared and distributed on each occasion. one set comprised six swabs dipped in pig faeces spiked with brachyspira spp. intended for diagnostics. the other set comprised two pure ... | 2005 | 15982833 |
| prevalence of lawsonia intracellularis, brachyspira hyodysenteriae and salmonella in swine herds. | the prevalence of lawsonia intracellularis, brachyspira hyodysenteriae and salmonella spp. were investigated by multiplex pcr using fecal samples of pigs with diarrhea or a history of diarrhea. the overall herd prevalence of l. intracellularis, b. hyodysenteriae and salmonella spp. were 46.5%, 37.2% and 51.1%, respectively. also, the prevalence of l. intracellularis, b. hyodysenteriae and salmonella spp. among all sampled pigs were 19.9%, 10.8% and 17.7%, respectively. seventeen of 43 herds were ... | 2005 | 16293991 |
| characterization of brachyspira hyodysenteriae isolates from korea. | this study was done to characterize diversity in 10 brachyspira hyodysenteriae isolates in korea. the isolates were compared with 14 well-characterized non-korean strains of various brachyspira species. all korean isolates showed strong beta haemolysis and had blunt cell ends with 7-14 periplasmic flagella. they produced indole, and did not ferment fructose. they were alpha-glucosidase positive and alpha-galatosidase negative using the apizym kit. using polyclonal antisera raised in rabbits agai ... | 2005 | 16293998 |
| the distribution of bmpb, a gene encoding a 29.7 kda lipoprotein with homology to metq, in brachyspira hyodysenteriae and related species. | the distribution of the bmpb gene encoding bmpb, a 29.7 kda outer membrane lipoprotein of the intestinal spirochaete brachyspira hyodysenteriae, was investigated. using pcr, the gene was detected in all the 48 strains of b. hyodysenteriae examined and in brachyspira innocens strain b256t, but not in 11 other strains of b. innocens nor in 42 strains of other brachyspira spp. the gene was sequenced from b. innocens strain b256t and from 11 strains of b. hyodysenteriae. the b. hyodysenteriae genes ... | 2005 | 15863284 |
| proposed revisions to the nomenclature for brachyspira membrane proteins and lipoproteins. | 2006 | 16385109 | |
| comparison of culture and biochemical tests with pcr for detection of brachyspira hyodysenteriae and brachyspira pilosicoli. | traditional culture and biochemical tests (cbt) were compared with pcr for sensitivity and detection of brachyspira hyodysenteriae and brachyspira pilosicoli in seeded faeces and clinical samples from diarrhoeic pigs. a duplex pcr system was developed based on primers detecting the tlya-gene of b. hyodysenteriae and the 16s rrna-gene of b. pilosicoli. sensitivities for the pcr system were determined on seeded faeces, using dna that had been recovered from primary cultures or extracted directly f ... | 2006 | 16457900 |
| improvement in motion efficiency of the spirochete brachyspira pilosicoli in viscous environments. | spirochetes are unique among swimming bacteria in terms of their lack of external flagella. they actively move in viscous environments, and, surprisingly, the swimming speed of the spirochete leptospira interrogans has been reported to increase with viscosity in methylcellulose solutions. many researchers consider that the presence of a loose, quasi-rigid network formed by linear polymer molecules is related to this strange phenomenon. one of the authors has proposed a theory that expresses this ... | 2006 | 16415052 |
| antimicrobial susceptibility testing of brachyspira intermedia and brachyspira pilosicoli isolates from australian chickens. | susceptibilities of predominantly australian isolates of the pathogenic intestinal spirochaetes brachyspira intermedia (n = 25) and brachyspira pilosicoli (n = 17) from chickens were tested in agar dilution against four concentrations each of the antimicrobials tiamulin, lincomycin, tylosin, metronidazole, tetracycline and ampicillin. based on available minimum inhibitory concentration (mic) breakpoint values for brachyspira hyodysenteriae or other gram-negative enteric veterinary pathogens, iso ... | 2006 | 16448937 |
| genetic and biochemical characterization of fus-1 (oxa-85), a narrow-spectrum class d beta-lactamase from fusobacterium nucleatum subsp. polymorphum. | previous studies have reported beta-lactamase-mediated penicillin resistance in fusobacterium nucleatum, but no beta-lactamase gene has yet been identified in this species. an f. nucleatum subsp. polymorphum strain resistant to penicillin and amoxicillin was isolated from a human periodontitis sample. dna cloning and sequencing revealed a 765-bp open reading frame encoding a new class d beta-lactamase named fus-1 (oxa-85). a recombinant escherichia coli strain carrying the bla(fus-1) gene exhibi ... | 2006 | 16870757 |
| smpb: a novel outer membrane protein present in some brachyspira hyodysenteriae strains. | a novel outer membrane protein-encoding gene was identified in brachyspira hyodysenteriae. the predicted protein, smpb, was encoded by a gene that contains regions of identity with that encoding the previously identified lipoprotein smpa. however, the majority of the reading frame encoding smpa and smpb share no detectable similarity. analysis of several strains revealed that b. hyodysenteriae harbours either smpa or the newly identified gene smpb, but not both. smpb encodes for a slightly large ... | 2006 | 16310325 |
| tiamulin resistance in porcine brachyspira pilosicoli isolates. | there are few studies on antimicrobial susceptibility of brachyspira pilosicoli, therefore this study was performed to investigate the situation among isolates from pigs. the tiamulin and tylosin susceptibility was determined by broth dilution for 93 and 86 porcine b. pilosicoli isolates, respectively. the isolates came from clinical samples taken in swedish pig herds during the years 2002 and 2003. the tylosin minimal inhibitory concentration (mic) was >16 microg/ml for 50% (n=43) of the isolat ... | 2006 | 16253666 |
| induction of differential immune reactivity to members of the flora of gnotobiotic mice following colonization with helicobacter bilis or brachyspira hyodysenteriae. | aberrant host immune responses to bacterial components of the resident microflora may initiate and perpetuate gastrointestinal inflammation. to investigate how microbial perturbation promotes host immunological responsiveness to commensal bacteria and contributes to the development of typhlocolitis, we selectively colonized defined (altered schaedler) flora c3h mice with either helicobacter bilis or brachyspira hyodysenteriae. following selective colonization, tissues were analyzed for gross/his ... | 2006 | 16698302 |
| isolation of brachyspira pilosicoli from weanling horses with chronic diarrhoea. | 2006 | 16699136 | |
| d-ribose utilisation differentiates porcine brachyspira pilosicoli from other porcine brachyspira species. | d-ribose utilisation was studied in 60 brachyspira pilosicoli strains and 35 strains of other brachyspira species, the majority of which were of porcine origin. utilisation of d-ribose was demonstrated indirectly by measuring the reduction in ph of densely inoculated tryptone-peptone broth supplemented with 7% foetal calf serum and 1% d-ribose. among b. pilosicoli strains, the mean reduction in ph units was 1.72 (range 0.95-2.28) in broth with d-ribose and 0.27 (range 0.10-0.40) in sugar-free co ... | 2006 | 16701624 |
| potential for zoonotic transmission of brachyspira pilosicoli. | 2006 | 16710961 | |
| prevalence of brachyspira species in pigs with diarrhoea in spain. | 2006 | 16714437 | |
| differential expression of the bhmp39 major outer membrane proteins of brachyspira hyodysenteriae. | the enteric, anaerobic spirochete brachyspira hyodysenteriae is the causative agent of swine dysentery, a severe mucohemorrhagic diarrheal disease of pigs that has economic significance in every major pork-producing country. recent investigation into potential vaccine candidates has focused on the outer membrane proteins of b. hyodysenteriae. bhmp39 (formerly vsp39) is the most abundant surface-exposed outer membrane protein of b. hyodysenteriae; its predicted gene sequence has previously been s ... | 2006 | 16714554 |
| treatment of a field case of avian intestinal spirochaetosis caused by brachyspira pilosicoli with tiamulin. | there has been much confusion over the significance of spirochaetes found in the caeca of laying hens and the impact they may have on egg production. in recent years, the situation has been made clearer and the presence of such species as brachyspira pilosicoli have been shown to cause a mild, chronic disease in both layers and breeders and to reduce egg production by reportedly 5%. in the united kingdom, a multi-age caged laying site with three separate flocks of approximately 12 000 birds each ... | 2006 | 16753612 |
| reclassification of serpulina intermedia and serpulina murdochii in the genus brachyspira as brachyspira intermedia comb. nov. and brachyspira murdochii comb. nov. | a reassessment was undertaken of published characteristics of the five species of anaerobic intestinal spirochaetes in the genus brachyspira (brachyspira aalborgi, brachyspira alvinipulli, brachyspira hyodysenteriae, brachyspira innocens and brachyspira pilosicoli) and the two species in the genus serpulina (serpulina intermedia and serpulina murdochii). comparisons were made of published descriptions, including phenotypic properties, grouping by multilocus enzyme electrophoresis, dna base compo ... | 2006 | 16627646 |
| the 23s rrna gene pcr-rflp used for characterization of porcine intestinal spirochete isolates. | using three reference strains of brachyspira hyodysenteriae (b204, b234, b169), one b. pilosicoli (p43/6/78), one b. murdochii (56-150), one b. intermedia (pws/a), one b. innocens (b256) and ten korean isolates, pcr-rflp analysis of dna encoding 23s rrna was performed to establish a rapid and accurate method for characterizing porcine intestinal spirochetes. consequently, b. hyodysenteriae and b. pilosicoli revealed different restriction patterns; however, the other three species shared the same ... | 2006 | 16871023 |
| comparison of prevalence and risk factors for faecal carriage of the intestinal spirochaetes brachyspira aalborgi and brachyspira pilosicoli in four australian populations. | this study examined the prevalence of the intestinal spirochaetes brachyspira aalborgi and brachyspira pilosicoli in different western australian (wa) populations. faecal samples included 287 from rural patients with gastrointestinal symptoms, comprising 142 from non-aboriginal and 145 from aboriginal people; 227 from recent healthy migrants to wa from developing countries; and 90 from healthy non-aboriginal individuals living in perth, wa. dna was extracted from faeces, and subjected to pcr ass ... | 2006 | 16638167 |
| intestinal spirochetosis and chronic watery diarrhea: clinical and histological response to treatment and long-term follow up. | the clinical significance of intestinal spirochetosis is uncertain, therefore the aim of the present paper was to assess the prevalence of histological intestinal spirochetosis in patients with and without chronic watery diarrhea and to evaluate its clinical relevance. | 2006 | 16872318 |
| comparative evaluation of molecular assays for the identification of intestinal spirochaetes from diseased pigs. | rapid identification of porcine brachyspira species is required in order to differentiate pathogenic from non-pathogenic species. the aim of our study was to compare a recently described genetic method based on polymerase chain reaction (pcr) and restriction fragment length polymorphism (rflp), nox rflp-pcr assay, and three species-specific pcrs described previously in the literature with a 16s rrna gene rflp-pcr discriminatory reference assay (16s rflp-pcr) for the identification of brachyspira ... | 2006 | 16879935 |
| development of a two-step nested duplex pcr assay for the rapid detection of brachyspira pilosicoli and brachyspira intermedia in chicken faeces. | avian intestinal spirochaetosis (ais) is an infection of the caeca and/or colo-rectum of laying and meat breeder hens caused by anaerobic intestinal spirochaetes of the genus brachyspira. ais can result in a variety of symptoms, including delayed and/or reduced egg production, and increased faecal water content. the two most commonly reported brachyspira species involved in ais are brachyspira pilosicoli and brachyspira intermedia, and their detection and identification can be difficult and time ... | 2006 | 16677782 |