Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [practical value of examining cellular immunity in human brucellosis]. | 1984 | 6489630 | |
| [immunization of ewes and she-goats against experimental brucella melitensis infection. a comparison of rev 1 and h 38 vaccines]. | 1984 | 6489637 | |
| control of ovine brucellosis in najdi sheep in saudi arabia. | a practical programme for control of ovine brucellosis in a najdi sheep flock at haradh and protection of sheep handlers from infection was implemented. haradh sheep were serologically tested three times at six-month intervals. of 2,721 sheep subjected to the first tests, 387 (14.2%) were positive reactors. at the second test 2,072 sheep were tested and 31 (1.5%) were positive. the third test was performed on 2,963 sheep and only nine (0.3%) positive reactors were found. of eight sheep handlers ... | 1984 | 6523581 |
| a note on adult sheep vaccination with reduced dose of brucella melitensis rev 1. | this work describes results obtained by vaccinating pregnant ewes from a brucella melitensis-infected flock, with 5 x 10(8) cfu (half dose) rev 1. only five abortions were recorded after vaccinating 600 pregnant ewes. the serological response was followed by rose bengal, complement fixation and radial immunodiffusion tests. a short-termed, low humoral immunoresponse was produced. the radial immunodiffusion test could be more useful than the other tests for differentiating infected from vaccinate ... | 1984 | 6529126 |
| infection with brucella melitensis apparently acquired in the united kingdom. | a patient with brucella melitensis presented to a clinic for sexually transmitted diseases. she had unusual clinical signs including polyarthropathy and a tender enlarged spleen. her disease was apparently acquired in the united kingdom. the clinical features and immunological aspects of brucellosis are discussed. | 1984 | 6542121 |
| transient hemophagocytosis in brucella melitensis infection. | bone marrow phagocytic activity was studied in 40 patients with brucella melitensis infection and 35 patients with a variety of medical conditions. evidence of erythrophagocytosis was found in 14 of the 40 patients with b melitensis infection and in 15 of the 35 control cases; this was taken as baseline bone marrow phagocytic activity. considerably increased erythrophagocytosis or phagocytosis of all blood elements by increased number of histiocytes was found in one and seven patients, respectiv ... | 1984 | 6546508 |
| brucellosis in a group of travelers to spain. | an epidemiologic investigation, initiated when brucella melitensis infection was detected in a high school student, identified five unrecognized cases in classmates. before the investigation, four infected students had symptoms of brucellosis for one to ten weeks, made nine visits to physicians, and were confined to the school infirmary or hospitals for 27 days. the other two students were asymptomatic when brucella agglutination testing demonstrated elevated titers, and treatment was quickly in ... | 1984 | 6690819 |
| [rifampin in osteoarthritis due to brucella melitensis]. | 1984 | 6714826 | |
| non-specific stimulation of human b lymphocytes by a brucella fraction. | pi, a lipid a-free fraction (15) extracted from the cell wall of brucella melitensis type i m.15 behaves as a non specific mitogen and a polyclonal b-cell activator (pba) for human unsensitized lymphocytes from subjects with no previous history and no biological stigmata of brucellosis. both effects may be evidenced, indifferently, in cultures of peripheral blood, tonsils or cord blood lymphocytes. mitogenicity, estimated by the incorporation of tritiated thymidine is maximal on the 5th and 6th ... | 1984 | 6208072 |
| [brucellosis: a varied clinical presentation in 9 patients]. | from 1973 to 1983 nine cases of brucella melitensis infection were hospitalised at the centre hospitalier universitaire vaudois (chuv), lausanne. in each case, the infection was acquired in a mediterranean country (4 cases in italy, 2 in spain, 2 in portugal and 1 in greece). in 6 of the cases the disease was acquired by ingestion of dairy products and in 2 cases by direct animal contact. despite classical initial symptomatology (fever, rigors, weakness), the time from first symptoms to diagnosi ... | 1984 | 6335295 |
| [somatic antigens of the brucella genus. the structure of the o-specific polysaccharide chain of brucella melitensis lipopolysaccharide]. | the phenol-phase soluble antigenic lipopolysaccharide was isolated from brucella melitensis, strain 565, by the routine phenol/water procedure followed by chromatography on sepharose 4b. after mild acid hydrolysis and chromatography on sephadex g-50, the lipopolysaccharide yielded a linear o-specific polysaccharide built up from 1,2-linked 4,6-dideoxy-4-formamido-alpha-d-mannopyranosyl units. the structure of the polysaccharide was deduced mainly from the nuclear magnetic resonance and methylati ... | 1985 | 2413867 |
| neurobrucellosis in an eight-year-old child. | an 8-year-old boy, presenting with signs of chronic meningitis, and involvement of cranial nerves, was found to have neurobrucellosis. the diagnosis was rapidly established by an enzyme-linked immunosorbent assay (elisa) that detected high titres of igg, iga and igm brucella antibodies in csf and in serum. brucella melitensis was subsequently isolated both from blood and csf. neurobrucellosis, which has been considered to be very rare in childhood, should be suspected in cases of meningitis of o ... | 1985 | 2418766 |
| ovine brucellosis: repeated vaccination with rev 1 vaccine and the prevalence of the disease in the winburg district. | the effect of repeated vaccination of ram lambs with brucella melitensis rev 1 vaccine was studied in relation to complement fixation titres. the presence of brucella organisms in semen and changes in neutrophil densities of the semen were also studied. a survey of the incidence of ovine brucellosis in the winburg district on the basis of a clinical examination of rams and the complement fixation test is also reported on. repeated vaccination of ram lambs with rev 1 vaccine resulted in high comp ... | 1985 | 3836306 |
| brucella fractions behave as nonspecific mitogens and polyclonal b-cell activators for human lymphocytes. | two lipid-a-free fractions which were extracted from brucella melitensis and were designated pi and sf stimulated human unsensitized mononuclear cells to proliferate and to secrete immunoglobulins. both of these effects were observed in cultures of peripheral blood, tonsils, and cord blood lymphocytes. neither b cells nor t cells alone proliferated in the presence of these fractions, whereas the proliferative response of t cells plus b cells was largely independent of accessory cells. polyclonal ... | 1985 | 3876286 |
| [general mathematical methodology for analyzing bacterial biotope-biotype correspondence: application to the epidemiology of brucella melitensis in france]. | 1985 | 3879674 | |
| brucellar spondylitis: a detailed analysis based on current findings. | three hundred thirty-one cases of brucellosis included in a 10-year prospective protocol were reviewed to identify and follow up patients with spondylitis. of 20 patients (17 male and three female; mean age, 54 years), spondylitis was diagnosed soon after onset of the brucellosis in 15, there were significant systemic symptoms in 17, and blood cultures were positive for brucella melitensis in 14. the main symptom was vertebral pain. the commonest radiographic changes were narrowing of the disk a ... | 1985 | 3903944 |
| characterization of monoclonal antibodies against brucella melitensis. | 1985 | 3907209 | |
| phagocytosis and killing of brucella by human polymorphonuclear leukocytes. | although cellular immunity involving activated macrophages is important in resistance to brucella, serum factors and polymorphonuclear leukocytes (pmnls) play some role in the initial response to infection. the interaction between human pmnls and virulent and attenuated strains of brucella abortus and brucella melitensis was studied by in vitro techniques. virulent and attenuated strains of both species were rapidly phagocytosed after opsonization with normal human serum (nhs); nonopsonized bact ... | 1985 | 3919118 |
| brucella melitensis pericarditis. | 1985 | 3993521 | |
| survey of brucellosis in goats and sheep in the yemen arab republic: comparison of tests for brucella melitensis infection in sheep. | sera from 538 yemeni goats and 690 yemeni sheep were screened for brucellosis by the rose bengal plate test (rbpt) and reactors confirmed by the complement fixation test (cft) and the serum agglutination test (sat). the prevalence among goats was 0.4% and among sheep 0.6%. the prevalence among 183 imported goats and sheep was 4.4%. the sensitivity and specificity of three serological tests available for the diagnosis of brucellosis -cft, rbpt and sat - were compared using ovine sera obtained thr ... | 1985 | 4024267 |
| isolation of brucella melitensis from sheep in syria. | 1985 | 4060527 | |
| comparative trial of rifampin-doxycycline versus tetracycline-streptomycin in the therapy of human brucellosis. | in an attempt to compare the efficacy of rifampin-doxycycline with tetracycline-streptomycin for the treatment of human brucellosis, we administered both combinations for a 30-day period, similar to the period recommended by the world health organization in a prospective, randomized trial. forty-six patients were included in the final study (36 men and 10 women); 41 had blood cultures positive for brucella melitensis. the 28 patients in group a received tetracycline hydrochloride at doses of 0.5 ... | 1985 | 4073878 |
| humoral immune abnormalities in human brucellosis. | humoral immune responses were evaluated in a group of patients with acute brucellosis due to brucella melitensis biotype i. levels of immunoglobulins, c3 and c4, and the presence of antinuclear antibodies, rheumatoid factor and circulating immune complexes were sequentially studied in all brucellosis patients during the acute phase of the disease and following specific therapy. elevated levels of all immunoglobulin classes, igg, igm and iga and c3, c4, were found in the majority of patients. ant ... | 1985 | 4083235 |
| vaccination of ewes by a single conjunctival administration of brucella melitensis rev. 1 vaccine. | in a lot of 99 ewes, the efficacy of a single vaccination by the conjunctival route (vc) with either 1.4 x 10(8) or 1.4 x 10(9) cfu brucella melitensis rev. 1 was compared with that of normal subcutaneous (sc) vaccination with 1.6 x 10(9) cfu. the ewes were challenged seven months after vaccination, when 85 days pregnant, with 5 x 10(7) cfu brucella melitensis strain h38 administered by conjunctival route. complement fixation, rose bengal and allergic tests were performed at intervals between va ... | 1985 | 4091489 |
| brucellosis: serological methods compared. | at least 12 persons contracted clinical, and 4 persons subclinical brucella melitensis infection during a brucellosis epidemic in the spring and summer of 1983 in southern germany, a region which had been free of this disease for the past 20 years. all cases of illness were traced to one infected herd of sheep. the presence of antibodies against b. melitensis was examined in 72 sera of infected patients using the following tests: agglutination, coomb's test, two complement fixation tests with di ... | 1985 | 4093607 |
| relevance of in vitro antimicrobial susceptibility of brucella melitensis to relapse rate in human brucellosis. | the in vitro susceptibility of brucella melitensis was examined vis-a-vis the clinical outcome in 75 patients with brucellosis. the initial mics for brucella isolates from patients who relapsed and from those who did not were similar. furthermore, the mics for isolates from patients whose infections relapsed were no different from those for original isolates. our results clearly showed that neither initial nor subsequent antibiotic susceptibility plays a role in the likelihood of relapse of pati ... | 1986 | 3813520 |
| an evaluation of diagnostic methods for brucellosis--the value of bone marrow culture. | fifty patients, eventually diagnosed as having brucellosis, were studied prospectively for comparison of a simple culture of bone marrow aspirate from the iliac crest (0.5-1.0 ml) with two cultures of blood (5-10 ml) taken 30-60 min apart and with a tube-agglutination test. cultures of bone marrow and blood were positive in 92% and 70% of the patients, respectively (p less than .001). bacteria multiplied significantly faster in bone marrow cultures (4.32 vs. 6.65 days; p less than .001). all iso ... | 1986 | 3941276 |
| [lumbosacral meningoradiculitis as a late manifestation of brucellosis (brucella melitensis)]. | neurologic complications can occur during the course of brucellosis. in a 25-year-old patient admitted july 3, 1984 because of loss of weight (10 kg) brucellosis (brucella melitensis) was diagnosed on the basis of splenomegaly, positive agglutination and positive blood and bone marrow cultures. therapy with cotrimazol was performed for eight weeks. on july 31, 1984 the patient was readmitted because of weakness in both legs. neurologic examination revealed slight weakness of the hip extensors an ... | 1986 | 3952482 |
| intracellular killing of brucella melitensis within mouse peritoneal macrophages: influence of treatment with rifampicin. an ultrastructural study. | the aggregation and condensation of ribosomes and the disjunction of the cell-wall membranous system are the ultrastructural alterations caused by rifampicin on b. melitensis cultured in brucella-broth medium. our ultrastructural researches carried out on mouse peritoneal macrophages infected with b. melitensis and treated with rifampicin (1 microgram/ml) have demonstrated that vacuoles containing b. melitensis which had been damaged by the drug fuse with lysosomes. on the contrary vacuoles cont ... | 1986 | 3713542 |
| demonstration of antibodies against brucella melitensis 16m lipopolysaccharide and native hapten in human sera by enzyme-linked immunosorbent assay. | an enzyme-linked immunosorbent assay (elisa) for the detection and quantification of human immunoglobulin g (igg), iga, and igm antibodies against brucella melitensis 16m by using lipopolysaccharide (lps) and native hapten (nh) as antigens is described. the results obtained with the lps elisa were compared with the results of the nh elisa. a good statistically significant correlation was established between the antibody titers of the igg class against both antigens. a total of 104 (99%) of the 1 ... | 1986 | 3722367 |
| in vitro susceptibility of brucella melitensis to new cephalosporins crossing the blood-brain barrier. | the in vitro susceptibilities of 83 clinical isolates of brucella melitensis to seven cephalosporins and a monobactam were determined. ceftizoxime, ceftriaxone, and cefotaxime were the most effective agents tested, with mics ranging from 0.25 to 2 micrograms/ml. moxalactam, cefoperazone, cefuroxime, and ceftazidime showed mics between 4 and 64 micrograms/ml, with moxalactam being the most active agent in this group. aztreonam showed poor activity, with mics higher than 64 micrograms/ml. | 1986 | 3729331 |
| cardiac manifestations in brucellosis. | cardiac involvement in childhood brucellosis is rare and when present mimics findings usually noted in acute rheumatic fever with carditis. we report five children aged 6 to 11 years. echocardiography showed mitral valve vegetations in one, functional mitral valve incompetence in two, and sluggish myocardial function in one. all the patients presented with fever, arthralgia, and malaise, four of them had leucopenia, and all five showed relative lymphocytosis. blood cultures grew brucella meliten ... | 1986 | 3729526 |
| brucella arthritis in children. | a study in 50 children suffering from acute brucellosis demonstrated that acute arthritis is a common and often predominant manifestation of the disease. the patients lived in an area where brucellosis is endemic: their ages ranged from six months to 12 years. all patients had a brucella agglutination titer of greater than or equal to 1:320 on admission. the knee and hip joints were most commonly affected with symptoms and findings from only one joint predominating. associated findings of only m ... | 1986 | 3793238 |
| evaluation of elisa in the diagnosis of acute and chronic brucellosis in human beings. | an enzyme-linked immunosorbent assay (elisa) was used to determine the levels of brucella-specific igg, igm and iga in 173 patients with acute brucellosis, 22 patients with chronic brucellosis and in 281 controls consisting of 98 patients with other infectious etiologies, 20 patients with non-infectious diseases and 163 normal healthy adults. the elisa results were compared with culture findings, the results of slide agglutination tests with brucella melitensis (m), b. abortus (a) and ross benga ... | 1986 | 3794323 |
| urban outbreak of goat cheese brucellosis. | in 1983 an outbreak of human brucellosis caused by brucella melitensis occurred among residents of a predominantly hispanic neighborhood in houston, tx. the source of the infections was traced to unpasteurized goats' milk cheese imported from mexico. nineteen of the 31 patients who contracted the disease were members of 5 families, which included 11 children ranging in age from 2 to 17 years. infection was generally associated with protein complaints and a paucity of physical findings. both immu ... | 1986 | 3797296 |
| rifampicin resistance in a strain of brucella melitensis after treatment with doxycycline and rifampicin. | 1986 | 3804888 | |
| inhibition of in vitro immunoglobulin synthesis by brucella melitensis. | 1986 | 3502547 | |
| [in vitro erythrophagocytosis in detecting opsonizing antibodies in a patient with malta fever. a hypothesis on the explanation of in vivo hemophagocytosis in other patients with malta fever]. | in vitro agglutination and phagocytosis of erythrocytes by homologous neutrophil granulocytes and monocytes could be observed using the blood of a healthy person (blood group a, rh+) to detect opsonising antibodies in the serum of a patient with malta fever (blood group o, rh+). agglutination and erythrophagocytosis were independent of complement or the presence of brucella melitensis. in patients with malta fever and blood groups o and b, respectively, antibodies could be detected on the surfac ... | 1986 | 3519436 |
| brucellosis--cause of abortion in sheep and its public health significance. | present study was undertaken to determine the association of brucellosis with abortions occurring naturally in sheep at an organized local sheep breeding farm. a total of 15 strains of brucella melitensis biovar i were isolated from the abortion material. serologically the aborted ewes were positive for brucellosis by one or more tests. during acute infection (abortion), standard tube agglutination test (sat) detected more positive reactors (70.7%) while counter immunoelectrophoresis (cie) detec ... | 1986 | 3557828 |
| in-vitro activity of ciprofloxacin, ceftriaxone and five other antimicrobial agents against 95 strains of brucella melitensis. | we studied the in-vitro activity of seven antibiotics against 95 strains of brucella melitensis isolated in blood cultures of 95 patients with brucellosis. the minimum inhibitory concentration (mic) was measured by the agar dilution method. all strains of b. melitensis were inhibited by doxycycline at 0.25 mg/l, tetracycline at 0.5 mg/l, ciprofloxacin at 0.5 mg/l, streptomycin at 1 mg/l, ceftriaxone at 1 mg/l, rifampicin at 4 mg/l and by co-trimoxazole at 0.5/9.5 mg/l. we did not find strains re ... | 1986 | 2940209 |
| evaluation of blood clot cultures for isolation of salmonella typhi, salmonella paratyphi-a, and brucella melitensis. | two types of clot culture, one with taurocholate-streptokinase and the other with bile as a culture medium, and two conventional cultures of whole blood were evaluated in parallel in an area where typhoid fever and brucellosis are endemic. each of the four systems contained 5 ml of blood or the clot derived from 5 ml of blood and sufficient broth to yield a 1:11 dilution of the specimen. of 542 patients studied, salmonella paratyphi-a was isolated from 61, s. typhi from 46, and brucella melitens ... | 1986 | 3093527 |
| enzyme-linked immunosorbent assay with major outer membrane proteins of brucella melitensis to measure immune response to brucella species. | we developed an enzyme-linked immunosorbent assay (elisa) system to measure human immunoglobulin g (igg) and igm response to the major outer membrane proteins of brucella melitensis. the elisa was more sensitive in detecting antibody than a standard microagglutination (ma) test with b. abortus antigen. of 101 sera from persons with suspected brucellosis, 79 (78.2%) gave elisa igm titers greater than or equal to the b. abortus ma titer without 2-mercaptoethanol (2me), which measures both igm and ... | 1986 | 3095364 |
| antigenic differences between brucella abortus and brucella melitensis recognized by a monoclonal antibody. | by means of somatic hybridization of spleen cells from balb/c mice inoculated with brucella abortus and of a line of murine myeloma, a monoclone was obtained producing an antibody which specifically recognizes an epitope of brucella abortus by means of the agglutination reaction. this epitope is not only on the surface of the homologous strains but on all the brucella abortus biotype i, on the brucella melitensis biotype ii and on the brucella suis biotype ii tested. on the contrary it is not pr ... | 1986 | 2423851 |
| alimentary tract presentations of brucellosis. | a retrospective study was conducted to assess alimentary tract involvement in 140 saudi patients with acute or chronic brucellosis. a brucella standard agglutination titer of 160 or over was considered to be positive evidence of brucella infection. the commonest symptoms were anorexia, weight loss, right hypochondrial pain, and constipation. a history of raw milk ingestion was obtained in 38 patients. thirty-nine patients had hepatomegaly, 21 had splenomegaly, and six had signs of cholecystitis. ... | 1986 | 21164236 |
| brucella outer membrane lipoprotein shares antigenic determinants with escherichia coli braun lipoprotein and is exposed on the cell surface. | in an enzyme-linked immunosorbent assay (elisa), purified brucella abortus and escherichia coli peptidoglycan-linked lipoproteins gave a strong cross-reaction with sera from rabbits hyperimmunized with the heterologous lipoprotein. when smooth e. coli cells were used as elisa antigens, the immunological cross-reaction was not observed unless the cells were treated to remove lipopolysaccharide and other outer membrane components. in contrast, intact cells from smooth strains of b. abortus and bru ... | 1987 | 2432014 |
| monoclonal antibodies and characterization of epitopes of smooth brucella lipopolysaccharides. | four mouse monoclonal antibodies generated against brucella melitensis 16m, and three generated against b. suis 1330 were analysed. an elisa (enzyme-linked-immunosorbent assay) with whole cells as antigens was used to determine cross-reactivities with other gram-negative bacteria. two antibodies showed cross-reactivity with smooth brucella strains only. five antibodies also reacted with yersinia enterocolitica o:9, but not with other bacteria. two of these antibodies had significantly higher tit ... | 1987 | 2449902 |
| [determination of biovars of brucella melitensis by manometric character: epidemiological value and relation to serovars]. | as brucella melitensis is the most frequently encountered brucella species in human medicine, and as its subdivision into three serovars is insufficient for epidemiological studies, we sought to define a new scheme for biotyping of this species. for this purpose, we studied 94 strains for their oxidative metabolic rates on 32 substrates and their susceptibility to ten antibiotics, and 132 strains for their activities with three enzymes. a taxonomic study on the basis of the 32 oxidative metaboli ... | 1987 | 3111505 |
| characterization of a native polysaccharide hapten from brucella melitensis. | the 13c nuclear magnetic resonance spectrum of brucella melitensis native polysaccharide hapten proved to be very similar to that generated by the o-specific chain (ps) isolated from b. melitensis lipopolysaccharide; that is, to a linear polymer in which the repeating unit is composed of five n-formylperosaminyl residues, one of them being substituted at position c-3 and the other four at position c-2. the serological analysis suggests that the so-called a determinant is present solely in brucel ... | 1987 | 3117696 |
| [molecular nature of the brucella polysaccharide antigen (poly-b)]. | cyclic (1----2)-beta-d-glucan was isolated from killed cells of pathogenic brucella melitensis 16m. its structure was deduced mainly from the acid hydrolysis, methylation analysis and 13c-nmr spectroscopy data. the cycloglucan and demicellated lipopolysaccharide of b. melitensis 16m form a stable complex identical, by immunodiffusion test, to the earlier described polysaccharide b antigen. | 1987 | 3118886 |
| identification of the a and m antigens of brucella as the o-polysaccharides of smooth lipopolysaccharides. | rabbit antisera, cross-absorbed serotype-specific for the brucella a and m antigens, precipitated respectively the smooth lipopolysaccharides from b. abortus 1119-3 and b. melitensis 16m. the antigenic a and m activity of these lipopolysaccharides was shown to reside within the o-chain region of the smooth lipopolysaccharides by inhibition experiments. homologous o-polysaccharides showed the highest inhibitory activity in elisa, although both the a and m antigens were active as heterologous inhi ... | 1987 | 3129169 |
| enzyme-linked immunosorbent assay for diagnosis of brucellosis. | the performance of an elisa for detection of total antibodies to brucella spp. was compared with that of the rose bengal, standard agglutination and coombs test in the diagnosis of brucellosis. sera tested were from 208 patients from whom brucella melitensis had been isolated, 177 patients with significant results in at least two conventional tests, and 107 patients with fever from whom no brucella spp. had been isolated and in whom all conventional tests were negative. elisa was the most sensit ... | 1987 | 3569256 |
| simultaneous vaccination by three living attenuated strains of brucella, salmonella and chlamydia in mice. | associations of vaccines for simultaneous administration were successfully developed long ago, in particular in human medicine, although there have been very few attempts to associate living bacterial vaccines. unfortunately, living vaccines can interact with one another resulting in either a virulent infection or an exclusion of one by the other. having developed two new low virulence vaccinal strains of chlamydia psittaci var. ovis and salmonella abortus ovis for use in veterinary medicine, we ... | 1987 | 3577354 |
| the structure of the lipopolysaccharide o-chain (m antigen) and polysaccharide b produced by brucella melitensis 16m. | the surface m antigen of brucella species has been identified as the lipopolysaccharide o-polysaccharide component composed of a repeating pentasaccharide unit containing a sequence of one 1,3- and four 1,2-linked, 4,6-dideoxy-4-formamido-alpha-d-mannopyranosyl units. a neutral polysaccharide produced by brucella species and referred to as polysaccharide b (poly b) has been identified as a family of circular 1,2-linked polymers of beta-d-glucopyranosyl units ranging in ring size from 17 to 24 gl ... | 1987 | 3582676 |
| immunochemical identification of antigens of brucella melitensis by means of crie. | a crude extract of brucella melitensis was obtained by sonication, centrifugation and dialysis, and analyzed by quantitative immunoelectrophoresis. cie analysis, using a high titer purified rabbit antibody anti-brucella fraction, showed that the brucella melitensis extract contained at least 22 antigens that all moved towards the anode. the antigens of the crude extract able to interact with igg of patients affected by brucellosis, were identified by means of crie using sheep 125i-anti human igg ... | 1987 | 3587065 |
| prosthetic valve endocarditis caused by brucella melitensis. a report of four cases successfully treated with tetracycline, streptomycin, and sulfamethoxazole and trimethoprim plus valve replacement. | optimal treatment of prosthetic valve endocarditis due to brucella melitensis is unknown. the presence of ring abscess makes extensive surgical débridement and valve replacement essential steps of management. antimicrobial therapy with tetracycline hydrochloride plus streptomycin sulfate or sulfamethoxazole and trimethoprim can achieve the sterilization of infected cardiac tissue. late bland periprosthetic leaks occur frequently. reoperation can be safely performed after a period of antimicrobia ... | 1987 | 3592879 |
| sacroiliitis as a sole manifestation of brucella melitensis infection in a child. | a case of a 12-year-old boy with sacroiliitis documented by positive tc-99m mdp and ga-67 scans is described. isolation of brucella melitensis from the blood and bone marrow established the diagnosis. he responded promptly to docycycline therapy. throughout the course of his disease this boy had neither fever nor other signs of brucellosis, and x-ray was normal. | 1987 | 3595029 |
| brucella melitensis meningitis following head trauma. | 1987 | 3596810 | |
| brucellar arthritis: a study of 39 peruvian families. | a study was conducted to characterise the articular manifestation of brucella melitensis within a family in peru. from january 1981 to june 1986, 39 families with 232 individuals were evaluated. brucellosis was diagnosed in 118 family members (attack rate of 50.9%). a lower attack rate was observed in children less than 10 years' old compared with other age groups (p less than 0.02). complete clinical data were available in 92 of the 118 affected members. moderate and severe forms of the disease ... | 1987 | 3662637 |
| [a study of identification of two atypical strains of brucella melitensis]. | 1987 | 3664616 | |
| infective aortitis due to brucella melitensis. | infective aneurysms caused by brucella sp. are extremely rare. b. suis and b. abortus have been implicated in a few cases but to our knowledge, b. melitensis has not been reported as a cause of mycotic aneurysm of the abdominal aorta. we here report the first case of this described in the english literature. the patient was successfully treated with resection of the infected aneurysm, placement of an axillo-bifemoral graft, and prolonged antibiotic treatment (doxycycline and streptomycin). exten ... | 1987 | 3672053 |
| immunization with brucella melitensis rev 1 against brucella ovis infection of rams. | the efficacy of brucella melitensis rev 1 vaccine (rev 1) for the prophylaxis of brucella ovis ram epididymitis was evaluated. twenty-nine 3-month-old rams were vaccinated with 2 x 10(9) rev 1 and 14 were revaccinated with 5 x 10(8) at 14 months of age. six rams remained unvaccinated as a control group. all rams were challenged with 5 x 10(8) b. ovis at 21 months of age. before being slaughtered 8 weeks later, only one vaccinated ram developed epididymitis while four of the six control rams deve ... | 1987 | 3686824 |
| cryoglobulinemia and cutaneous vasculitis in human brucellosis. | we report the occurrence of cryoglobulinemia and cutaneous vasculitis in three patients with brucellosis caused by brucella melitensis. the isolated cryoglobulins were characterized as mixed polyclonal or type iii. brucella agglutinin activity was not detected in any of the cryoglobulins analyzed. however, the same agglutinin titer and the presence of precipitin lines of identity in immunodiffusion gels were observed in the supernatant of the cryoglobulins and the serum. rheumatoid-factor activi ... | 1987 | 3693520 |
| control of brucella melitensis brucellosis in developing countries. | 1987 | 3300717 | |
| control of brucella melitensis infection in sheep and goats--a review. | 1987 | 3307078 | |
| risk of infection by brucella melitensis for people living near infected goats. | taking advantage of a local outbreak of brucellosis in a herd of goats, we have assessed the risk of infection for people living nearby. by means of serological tests we have shown that such a risk is small provided that direct contact with contaminated tissues or secretions of goats is avoided. | 1987 | 3312421 |
| [endocarditis with unusual causative agents]. | in the period 1947-1985, 601 patients with infective endocarditis were seen at the university hospital zurich and the kantonsspital lucerne. streptococci, enterococci and staphylococci were the predominant causative organisms in two-thirds of all cases. in more than 25% of the patients blood cultures remained negative. in 6 patients endocarditis was caused by very rare organisms, viz. coxiella burnetii (2 cases), hemophilus parainfluenzae, corynebacterium bovis (diphtheroids), brucella melitensi ... | 1987 | 3324331 |
| an outbreak of brucella melitensis infection by airborne transmission among laboratory workers. | an outbreak of acute brucellosis infection was detected among the employees of a biologicals manufacturing laboratory located in girona, spain. the first cases appeared six weeks after a vaccine with attenuated brucella melitensis, rev-1 had been produced for one week. a clinical and epidemiologic investigation conducted among the 164 employees found 22 patients with clinical symptoms and positive serology, and six patients detected by serology only (attack rate: 17.1 per cent). blood cultures w ... | 1987 | 3812841 |
| release of outer membrane fragments by exponentially growing brucella melitensis cells. | rough and smooth strains of brucella melitensis released a membranous material that was devoid of detectable nadh oxidase and succinic dehydrogenase activity (cytoplasmic membrane markers) but that contained lipopolysaccharide, proteins, and phospholipids. this material was composed of two fractions that had similar chemical compositions but that were of different sizes which were separated by differential ultracentrifugation. electron microscopy showed that both fractions are made of unit membr ... | 1987 | 3818086 |
| the spectrum of pneumonia in 1983 at the riyadh armed forces hospital. | the agents causing pneumonia have been assessed in 112 adult patients admitted to the armed forces hospital in riyadh during a period of one year: pathogens were identified in 78 patients (69.6%). sputum culture produced a significant isolate in 60 patients (53.5%), and in 17 (15.2%) the causative agent was suggested by serological tests. streptococcus pneumonia was the commonest infecting agent (21.4%). pneumonia due to mycobacterium tuberculosis was diagnosed in eight patients, to mycoplasma p ... | 1987 | 3819456 |
| structural elucidation of the brucella melitensis m antigen by high-resolution nmr at 500 mhz. | the brucella m antigen from the species type strain brucella melitensis 16m has been identified as a component of the cell wall lipopolysaccharide (lps). o polysaccharide liberated from this lps by mild acid hydrolysis exhibited m activity in serological tests and was shown to be a homopolymer of 4-formamido-4,6-dideoxy-alpha-d-mannopyranosyl residues arranged in an oligosaccharide repeating unit as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the native lipopolysacchar ... | 1987 | 3442684 |
| serological screenings of various infectious diseases on the cape verde islands (west africa). | general screening investigations with various antigens were carried out with a view to further specific investigations being carried out on the cape verde islands concerning infectious diseases. serological positive reactions were found in mumps, adeno, plt, cytomegaly, herpes, para-influenza 1, 2, 3, influenza a and b, mycoplasmosis, rs-virus, gonorrhoea, hepatitis a and b, r. conori, malaria, syphilis, brucella abortus, brucella melitensis, varicella, legionella, picornavirus, measles, german ... | 1987 | 3443744 |
| serological investigations in nigeria for anthropozoonoses in human sera: brucellosis, echinococcosis, toxoplasmosis, chlamydial diseases, listeriosis, rickettsiosis (coxiella burneti and rickettsia conori). | 176 blood sera taken from patients in the hospital of minna and abeokuta (nigeria) were examined for anthropozoonoses. the following positive reactions could be found: brucella abortus 9%, brucella melitensis 11.7%, echinococcosis 0.53%, toxoplasmosis 79.2% (cf-test 6.9%), chlamydial diseases 45%, listeriosis 28.7% (typ 1h) and 19.7% (typ 4bh), rickettsiosis--rickettsia conori 18.6% and coxiella burneti 63.3%. | 1987 | 3443751 |
| conjunctival vaccination of young goats with brucella melitensis strain rev 1. | three groups each of 15 four-month old female kids were vaccinated with brucella melitensis strain rev 1 either conjunctivally (1.1 x 10(7) or 1.1 x 10(9) cfu) or subcutaneously (1.1 x 10(9) cfu). one group of 15 kids remained unvaccinated as control. all kids were mated when 9.5 month old. three months later, they were challenged conjunctivally with b melitensis strain h38 at a dose of 5.1 x 10(6) cfu. blood samples collected before vaccination and throughout the experiment were subjected to ro ... | 1987 | 3451685 |
| brucella meningitis. | infection with brucella melitensis is endemic in saudi arabia but involvement of the central nervous system (cns) is rare. we report on three patients with acute brucella meningitis, all of whom had a history of exposure to a possible source of infection. diagnosis was confirmed by isolation of brucella species from blood cultures. examination of cerebrospinal fluid revealed lymphocytic pleocytosis with a high concentration of protein and low concentration of glucose. the patients were treated b ... | 1987 | 3494790 |
| osteoarticular complications of brucellosis: a study of 169 cases. | of 452 patients with brucellosis, 169 (111 male and 58 female) had osteoarticular complications. brucella melitensis was isolated from the blood in 7.7% of the cases. fever, chills, arthralgia, backache, high levels of c-reactive protein, positive rheumatoid factor, and splenomegaly were more frequent in osteoarticular brucellosis than in nonosteoarticular disease. arthritis occurred in the hip joint in 90 cases (53%), knees in 61 (36%), sacroiliacs in 33 (20%), ankles in 25 (15%), elbows in nin ... | 1987 | 3496650 |
| influence of autoclaved brucella melitensis on the proliferation of human lymphocytes. | brucella melitensis is a facultative intracellular bacterium which binds spontaneously to human b-lymphocytes. in order to find out whether there exists a relation between bacterial adherence and lymphocyte reactivity, the proliferation response of normal adult peripheral blood lymphocytes to autoclaved b. melitensis has been studied. our results indicate that autoclaved b. melitensis induce only a slight increase in proliferation. this low lymphocyte response is due to proliferating b-cells. in ... | 1987 | 3499149 |
| acute brucella ileitis. | human brucellosis is an enteric fever in which systemic symptoms are generally more prominent than gastrointestinal complaints. during an outbreak of infection caused by brucella melitensis, linked to ingestion of unpasteurized goat milk cheese, we treated a child with radiographic evidence of acute ileitis. alimentary tract involvement in brucellosis is reviewed. | 1988 | 3337066 |
| elisa versus routine tests in the diagnosis of patients with systemic and neurobrucellosis. | sera from patients in different stages of brucellosis as well as sera and cerebrospinal fluid (csf) from patients with central nervous system (cns) brucellosis and controls, were tested by elisa for brucella-specific igg, igm and iga. the results were compared with culture findings, micro-agglutination (ma), slide agglutination with rose bengal (rb), and brucella melitensis stained antigens (sa). in sera of patients with acute brucellosis (296), elisa was positive for igm (100%), igg (97%) and i ... | 1988 | 3345262 |
| the characteristics of a variant strain of brucella melitensis rev 1. | circumstantial evidence is presented for the occurrence of a variant of a vaccine strain of b. melitensis rev 1, designated "fsa" (foreign south african). fsa resembles rev 1 in its reactions to penicillin and streptomycin but reacts closer to a field strain of b. melitensis as regards dye (thionine and basic fuchsin) sensitivity and colony size. although colonies of rev 1 were consistently smaller than other b. melitensis strains, their size was 0,75 mm as opposed to the 1-2 mm reported in the ... | 1988 | 3353094 |
| characterization of brucella polysaccharide b. | polysaccharide b was extracted from brucella melitensis 16m and from a rough strain of brucella abortus 45/20 by autoclaving or trichloroacetic acid extraction of whole cells and by a new method involving mild leaching of cells. the material obtained by either of the established procedures was contaminated by o polysaccharide. the new leaching protocol eliminated this impurity and provided a pure glucan, which was regarded as polysaccharide b. this polysaccharide was found by high-performance li ... | 1988 | 3356461 |
| recurrent epidermal cyst infection caused by brucella melitensis in a diabetic patient. | a presternal swelling diagnosed clinically as a sebaceous cyst in a 60-year-old diabetic patient was surgically drained, and the aspirated purulant material yielded growth of brucella melitensis. the swelling recurred four times and was drained on every occasion. the patient responded to surgical excision and antibrucella treatment. the histologic diagnosis was an epidermal cyst. | 1988 | 3384896 |
| probable transmission of brucellosis from breast milk to a newborn. | the first case of neonatal brucellosis is described. the baby was fed breast milk only when the mother became acutely ill with fever, arthralgia and malaise. at the same time the baby was febrile. blood cultures grew brucella melitensis in both mother and child. the brucella agglutination titre in the breast milk was 1:2560. both mother and child recovered during treatment with streptomycin. | 1988 | 3407006 |
| brucella abortus 1119-3 o-chain polysaccharide to differentiate sera from b. abortus s-19-vaccinated and field-strain-infected cattle by agar gel immunodiffusion. | purified brucella abortus 1119-3 and brucella melitensis 16m lipopolysaccharide o-chain polysaccharides were not precipitated in agar gel immunodiffusion by any of 24 sera from vaccinated cattle but were precipitated by 18 of 24 sera from infected cattle. this difference can be used to differentiate sera of cattle vaccinated with b. abortus s-19 from sera of some field-strain-infected cattle. | 1988 | 3133389 |
| [100 years of bacteriology: the history of the discovery of brucellosis. 2: discovery of the etiology of infectious abortion in cattle by the danish veterinary pathologist bernhard bang and proof of the relation of "micrococcus melitensis" and and "bacillus abortus" by the american microbiologist alice evans]. | 1988 | 3136596 | |
| enzyme-linked immunosorbent assay with brucella native hapten polysaccharide and smooth lipopolysaccharide. | brucella melitensis native haptens (nh) are polysaccharides identical to the o-side chain of the smooth lipopolysaccharide (s-lps) (e. moreno, h. mayer, and i. moriyón, infect. immun. 55:2850-2853, 1987) which precipitate with sera from infected cattle but not from strain 19-vaccinated cattle. in the present work, nh was extracted by the hot-water method (r. díaz, j. toyos, m.d. salvo, and m.l. pardo, ann. rech. vet. 12:35-39, 1981) and purified free of s-lps and protein. purified nh lacked the ... | 1988 | 3147993 |
| [sanitary and technologic evaluation of the rural processing of fresh goat cheese in chile]. | a sanitary and technological diagnosis of the goat cheese rural process was carried out. the purpose was to obtain more information for the planning of a program aimed to the improvement of this small agroindustry. samples of milk, curdle, dry abomasum, rennet, water and cheese of 10% of the small industries of two rural villages in two agricultural seasons, were taken. moreover, dilutions of the utensils and goat udders were prepared. the samples were subjected to microbiological analysis of me ... | 1988 | 3154301 |
| [a rare cause of infection in chronic dialysis patients: malta fever (febris undulans melitensis)]. | a 45-years-old greek patient developed septicaemia in his 10th year of hemodialysis treatment. clinical investigation was directed first on bacterial infection of the arteriovenous shunt, on urosepsis or bronchopulmonary infection. then, serologically (widal's reaction and elisa-test) and in different blood-cultures brucella melitensis was detected as causative agent for malta fever (febris undulans). the focus of infection is suspected in unpasteurized sheep-milk cheese of mediterranean origine ... | 1988 | 3172682 |
| enzyme-linked immunosorbent assay for brucella melitensis-associated antigens. | an elisa assay was performed to detect antigens of brucella melitensis directly in the blood of patients affected by brucellosis. disposable polystyrene microtiter plates were coated with rabbit immunoglobulins anti-brucella melitensis antigens and then incubated with sera of brucellosis patients and sera of not infected normal subjects as a control, to standardize the conditions of the different steps of the assay. the level of the blood-containing brucella antigen bound to the plate was measur ... | 1988 | 3173124 |
| anti-chlamydial immunity in ewes conferred by vaccination with a combination of three live chlamydia, brucella and salmonella vaccines. | live attenuated vaccines against chlamydia psittaci var ovis, brucella melitensis and salmonella abortus ovis have previously been shown to be compatible in mice by subcutaneous administration. immunity against challenge with virulent chlamydia was, however, slightly decreased in associations including the b melitensis rev 1 vaccine. the chlamydia strain 1b vaccine was administered to four- to five-month-old female lambs, either alone or in combination with the b melitensis rev1 and the s abortu ... | 1988 | 3176252 |
| the isolation and serology of the "fsa" brucella melitensis rev. 1 mutant in a flock of sheep. | a flock of sheep, known to be infected with the "fsa" mutant of brucella melitensis rev. 1, was examined serologically and bacteriologically to determine whether any relationship existed which would help in the control of this infection in the field. an attempt was also made to determine whether vertical transmission occurred. twenty-one out of 62 sheep were bacteriologically positive. the best organs for isolation were the udder, supramammary lymphnodes and uterus. no significant relationship c ... | 1988 | 3194120 |
| brucellar sternoclavicular arthritis, the forgotten complication. | of 511 cases of brucellosis studied between december 1983 and february 1986, four (0.8%) had sternoclavicular (stcl) arthritis. two were male and two female, and only one was younger than 50 years old. all four cases had significantly high specific igg antibody titres (1 of 1280), measured by the indirect immunofluorescent (iif) test, and two had brucella melitensis isolated from their blood. in two cases, stcl arthritis was the presenting problem, and it was associated in one with ankle arthrit ... | 1988 | 3250341 |
| acute hepatitis and brucella melitensis infection: clinicopathological findings. | the clinical and pathological findings in a patient who had acute hepatitis caused by brucella melitensis are described. antibiotic therapy induced a good clinical and biochemical response, although a relapse occurred. brucellosis must be considered in the differential diagnosis of pyrexia of unknown origin, particularly if associated hepatic involvement is present. a careful occupational and travel history is essential. | 1988 | 3259669 |
| polyclonal igg synthesis of mononuclear cells induced by the homopolymer of 4,6-dideoxy-4-formamido-alpha-d-mannopyranosyl units isolated from brucella melitensis. | brucella melitensis attaches selectively to b lymphocytes. the bacterium induces in vitro only a slight proliferation of mononuclear cells and inhibits the spontaneous igg synthesis. in order to characterize these effects, the o-chain of the lipopolysaccharide of brucella melitensis (a polysaccharide consisting of poly-4,6-dideoxy-4-formamido-alpha-d-mannoside) was prepared and its influence on lymphocyte functions tested. this compound had no effect on blast transformation whereas igg synthesis ... | 1988 | 3266469 |
| brucellosis in the upper nile region--melut district, sudan (report). | in the melut district, south sudan human and animal blood samples were investigated for brucella abortus and brucella melitensis. 9.2% of the investigated cattle and 16.2% of the green long-tailed monkies were brucella abortus-positive. school children revealed a 3.7% positivity rate, melut's hospital patients 5.2% and village children and adults 2.2%. however, only a small number of hospital patients was investigated. a small percentage of sheep and goats was investigated for brucella melitensi ... | 1988 | 3275090 |
| review of pulmonary brucellosis: a case report on brucellar pulmonary empyema. | in certain geographic areas, among which most south european countries are included, brucellosis continues to be an important health problem. despite the disease's continued high prevalence, the advances made in its diagnosis and treatment have contributed to the rarity of different focal locations, such as the lung. formerly, these occurrences were much more frequent. the present work offers a review of the literature on pulmonary brucellar affection and describes a case report of brucellar pul ... | 1988 | 3064958 |
| a modified indirect fluorescent antibody test for the diagnosis of brucellosis. | we adapted the conventional indirect fluorescent antibody (ifa) test to assay igm and igg brucella-specific antibodies to differentiate acute from chronic infections rather than measure total antihuman globulin specific antibodies. the results were compared with the slide agglutination test (sat) used for screening and the quantitative microagglutination test (mat). of a total of 118 randomly selected samples sent for anti-brucella antibodies received at a general hospital laboratory, 58 (47.9%) ... | 1988 | 3071447 |
| definition of brucella a and m epitopes by monoclonal typing reagents and synthetic oligosaccharides. | the paradigm that brucella a and m epitopes are simultaneously expressed on single cells and within one antigen molecule was reinvestigated by using polysaccharide-specific murine monoclonal antibodies. monoclonal antibodies were generated to the m antigen of brucella melitensis 16m. chemically defined lipopolysaccharides and o polysaccharides from brucella abortus 1119-3, b. melitensis 16m, and yersinia enterocolitica o:9 were used to dissect the binding profiles of the b. melitensis antibodies ... | 1989 | 2474505 |
| analysis of brucella lipopolysaccharide with specific and cross-reacting monoclonal antibodies. | monoclonal antibodies which bind brucella a lipopolysaccharide (lps)-specific, m lps-specific, or cross-reactive epitopes were used as reagents in quantitative dot blot, western blot (immunoblot), and immunoprecipitation analysis of brucella whole cells, whole-cell extracts, and purified lps preparations. this set of monoclonal antibodies detected four unique epitopes on brucella lps. the specificity of monoclonal antibodies reactive with brucella unique (a and m) and common (c and c/y) lps epit ... | 1989 | 2479655 |
| dot-enzyme immunoassay for visual detection of brucella melitensis antibodies. | an indirect enzyme-linked immunosorbent assay, using antigen coupled to paper, has been adapted for the detection of brucella melitensis antibodies. optimum conditions were achieved by incubation of 1 ml of diluted serum with a single piece of paper coated with purified brucella antigens for a period of one hour, and by addition of a goat anti-human enzyme conjugate antibody for one hour again. under these conditions 80 human sera were examined and the results obtained were compared with wright ... | 1989 | 2490400 |
| brucellosis in the gezira area, central sudan. | twenty-nine patients presenting with symptoms and signs suggestive of brucellosis in the gezira area, central sudan, were studied. the majority of the patients (76%) were found to have a combined infection of both brucella abortus and brucella melitensis with titres of 1/160 and above. the social habit of eating raw meat, e.g. raw liver or other offal with spices (marrara or umfitfit) is found to be an important epidemiological factor in contracting the disease. raw milk consumption, since it is ... | 1989 | 2496237 |