Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| the effects of extracts from periodontopathic bacteria on human periodontal fibroblasts stimulated with mineralization supplements. | bacterial effects on in vitro mineralization of human periodontal fibroblasts (hpf) have not yet been examined in great detail. in our study, we investigated the effects of soluble extracts of the periodontopathic bacteria porphyromonas gingivalis, bacteroides forsythus and, treponema denticola on cell proliferation, mineralization, as well as on osteoblastic markers present in hpf cultured in vitro, such as alkaline phosphatase (alp) activity and collagen content. periodontal fibroblasts stimul ... | 2003 | 14650577 |
| prevalence of periodontopathic bacteria in aggressive periodontitis patients in a japanese population. | actinobacillus actinomycetemcomitans is considered a major etiologic agent of aggressive periodontitis. other periodontopathic bacteria such as porphyromonas gingivalis are also suspected of participating in aggressive periodontitis, although the evidence is controversial. the aim of the present study was to investigate the prevalence of periodontopathic bacteria and to clarify the microbiological features of aggressive periodontitis in japanese patients. | 2003 | 14653392 |
| potential role of vascular endothelial growth factor, interleukin-8 and monocyte chemoattractant protein-1 in periodontal diseases. | host-mediated immunoinflammatory pathways activated by bacteria lead to destruction of the periodontal connective tissues and alveolar bone. the objective of this study was to elucidate the activation of the inflammatory processes in periodontal disease by quantitative assessment of cytokines and periodontopathogens. gingival crevicular fluids (gcf) and subgingival plaque samples were collected from patients with chronic periodontitis and gingivitis and from periodontally healthy sites. vascular ... | 2003 | 12962428 |
| distribution of periodontal pathogens in korean aggressive periodontitis. | microbial associations in aggressive periodontitis versus different ethnic origins are substantially unknown. we undertook this study to determine the prevalence of seven putative periodontopathogens in korean patients and to evaluate microbial differences in localized and generalized aggressive periodontitis patients. | 2003 | 14584866 |
| flavobacterium johnsoniae gldh is a lipoprotein that is required for gliding motility and chitin utilization. | cells of flavobacterium johnsoniae move rapidly over surfaces by gliding motility. the mechanism of this form of motility is not known. six genes (glda, gldb, gldd, gldf, gldg, and ftsx) that are required for gliding have been described. tn4351 mutagenesis was used to identify another gene, gldh, which is required for cell movement. gldh mutants formed nonspreading colonies, and individual cells lacked the cell movements and ability to propel latex spheres along their surfaces that are character ... | 2003 | 14594839 |
| gingival crevice microbiota from chinese patients with gingivitis or necrotizing ulcerative gingivitis. | the objective of this study was to quantitatively compare the bacterial population structure in plaque from the gingival margin of two groups of 21 chinese patients with gingivitis or necrotizing ulcerative gingivitis (nug). subjects were recruited in four dental clinics in eastern china. samples were quantitatively assessed by immunofluorescence and fluorescent in situ hybridization for taxa known to be associated with periodontal diseases. the analyses showed that the fusiform taxa (fusobacter ... | 2004 | 14871191 |
| correlation between detection rates of periodontopathic bacterial dna in coronary stenotic artery plaque [corrected] and in dental plaque samples. | utilizing pcr, the 16s rrna detection rates for porphyromonas gingivalis, actinobacillus actinomycetemcomitans, bacteroides forsythus, treponema denticola, and campylobacter rectus in samples of stenotic coronary artery plaques were determined to be 21.6, 23.3, 5.9, 23.5, and 15.7%, respectively. the detection rates for p. gingivalis and c. rectus correlated with their presence in subgingival plaque. | 2004 | 15004106 |
| quadrant root planing versus same-day full-mouth root planing. | the aim of this study was to determine whether same-day full-mouth scaling and root planing (fm-srp) and quadrant scaling and root planing (q-srp) resulted in variations in the systemic humoral immune response dynamics (antibody titres and avidity) during active treatment and 3 and 6 months post-therapy. | 2004 | 15016017 |
| debridement and local application of tetracycline-loaded fibres in the management of persistent periodontitis: results after 12 months. | the aim of our study was to evaluate the clinical, radiological and microbiological response to the local delivery of tetracycline (te) of sites with persistent periodontal lesions. | 2004 | 15016019 |
| quadrant root planing versus same-day full-mouth root planing. ii. microbiological findings. | the aim of this study was to test the hypothesis that over a period of 6 months, same-day full-mouth scaling and root planing (fm-srp) resulted in greater reductions in the detection frequency of five putative periodontal pathogens compared with quadrant scaling and root planing (q-srp) in chronic periodontitis patients. | 2004 | 15016040 |
| biological activities of bacteroides forsythus lipoproteins and their possible pathological roles in periodontal disease. | bacteroides forsythus is a gram-negative, anaerobic, fusiform bacterium and is considered to be an etiological agent in periodontal disease. a lipoprotein fraction prepared from b. forsythus cells by triton x-114 phase separation (bflp) activated human gingival fibroblasts and a human monocytic cell line, thp-1, to induce interleukin-6 production and tumor necrosis factor alpha production. bflp was found to be capable of inducing nuclear factor-kappab translocation in human gingival fibroblasts ... | 2004 | 14977934 |
| quantitative detection of periodontal pathogens using real-time polymerase chain reaction with taqman probes. | quantitative analysis, with identification of periodontopathic bacteria, is important for the diagnosis, therapeutic evaluation and risk assessment of periodontal disease. we developed a highly sensitive and specific method using real-time polymerase chain reaction (pcr) to detect and quantify six periodontal bacteria: porphyromonas gingivalis, tannerella forsythia, actinobacillus actinomycetemcomitans, treponema denticola, prevotella intermedia, and prevotella nigrescens. species-specific taqma ... | 2004 | 15107068 |
| herpesviral-bacterial interrelationships in aggressive periodontitis. | recent findings have begun to provide a basis for a causal link between herpesviruses and aggressive periodontitis. one theory is that herpesviruses cooperate with specific bacteria in the etiopathogenesis of the disease. this study examined whether the presence of herpesviruses [human cytomegalovirus (hcmv), epstein-barr virus (ebv) type 1, herpes simplex virus (hsv) type 1 and 2] is associated with the presence of putative pathogenic bacteria (porphyromonas gingivalis, prevotella intermedia, t ... | 2004 | 15206912 |
| subgingival microbiota of chilean patients with chronic periodontitis. | an association between race/ethnicity and the composition of the subgingival microbiota has been found in chronic periodontitis. a study was undertaken to determine the characteristics of the subgingival microbiota of chronic periodontitis in chileans residing in santiago. | 2004 | 15212354 |
| quantitative microbiological study of subgingival plaque by real-time pcr shows correlation between levels of tannerella forsythensis and fusobacterium spp. | a taqman-based real-time pcr assay was established to quantify the periodontopathic bacteria tannerella forsythensis and fusobacterium spp. with this assay, the prevalence and proportion of these bacteria in clinical specimens were evaluated. our preliminary results suggest a positive colocalization of t. forsythensis and fusobacterium spp. in periodontal pockets. | 2004 | 15131205 |
| changes in oral microbial profiles after periodontal treatment as determined by molecular analysis of 16s rrna genes. | terminal rflp (t-rflp) analysis was used to investigate changes in the oral microbiota in saliva and subgingival plaque samples from one patient with aggressive periodontitis (subject a) and two patients with chronic periodontitis (subjects b and c) before and 3 months after periodontal treatment. substantial changes in the t-rflp patterns of subgingival plaque samples of subjects b and c were noted after 3 months of improved oral hygiene and full-mouth supra- and subgingival scaling and root pl ... | 2004 | 15150339 |
| polymerase chain reaction identification of microorganisms in previously root-filled teeth in a south korean population. | a large body of evidence indicates that microorganisms are the primary causative agents of endodontic treatment failures. this study intended to assess the occurrence of nine putative endodontic pathogens in root-filled teeth associated with periradicular lesions in a south korean population using a culture-independent molecular approach. fourteen root-filled teeth with persistent periradicular diseases were selected for retreatment. after removal of the root canal filling, the canals were sampl ... | 2004 | 15220647 |
| multiple functions of the leucine-rich repeat protein lrra of treponema denticola. | the gene lrra, encoding a leucine-rich repeat protein, lrra, that contains eight consensus tandem repeats of 23 amino acid residues, has been identified in treponema denticola atcc 35405. a leucine-rich repeat is a generally useful protein-binding motif, and proteins containing this repeat are typically involved in protein-protein interactions. southern blot analysis demonstrated that t. denticola atcc 35405 expresses the lrra gene, but the gene was not identified in t. denticola atcc 33520. in ... | 2004 | 15271922 |
| contribution of periodontal pathogens on tongue dorsa analyzed with real-time pcr to oral malodor. | oral malodor is considered to originate primarily from tongue microbiota populations. however, the relationship between oral malodor and tongue microbiota remains unclear. in this study, tongue periodontal pathogens were analyzed via real-time pcr, and the association between oral malodor and tongue periodontal pathogens, including porphyromonas gingivalis, tannerella forsythia, prevotella intermedia, prevotella nigrescens and treponema denticola, was examined. the subject population consisted o ... | 2004 | 15380777 |
| occurrence of porphyromonas gingivalis and tannerella forsythensis in periodontally diseased and healthy subjects. | the purpose of this study was to compare the prevalence and level of porphyromonas gingivalis (p. gingivalis) and tannerella forsythensis (t. forsythensis) in subgingival plaque samples from both healthy individuals and periodontal patients in different age groups. | 2004 | 15455734 |
| possible periodontal pathogens associated with clinical symptoms of periodontal disease in japanese high school students. | the purpose of this study is to investigate how the components of biofilm and clinical oral status change in adolescents and to identify specific periodontal pathogens as risk markers for the onset of periodontitis. | 2004 | 15455735 |
| clinical and microbiological effects of topical subgingival application of hyaluronic acid gel adjunctive to scaling and root planing in the treatment of chronic periodontitis. | hyaluronic acid (ha) has shown anti-inflammatory effects in gingivitis therapy. the potential benefits of local subgingival application of ha adjunctive to scaling and root planing (srp) were evaluated in this study. | 2004 | 15455740 |
| characterization of a bacteriocin produced by prevotella nigrescens atcc 25261. | to characterize the antimicrobial activity produced by prevotella nigrescens atcc 25261, and to evaluate its safety on cultured gingival fibroblasts. | 2004 | 15482437 |
| the effect of periodontal treatment on the salivary bacterial load and early plaque formation. | the purpose of the present study was to investigate in periodontitis patients the relationship between the number of bacteria in the saliva and the amount of de novo plaque formation before and after treatment. | 2004 | 15491312 |
| subgingival microbiota of chronic periodontitis subjects from different geographic locations. | most clinical studies assume that the subgingival microbiota is similar from one geographic location to another. the purpose of the present investigation was to examine the composition of the subgingival microbiota in chronic periodontitis subjects from four countries. | 2004 | 15491316 |
| the distribution of periodontopathic bacteria among japanese children and their parents. | it is not well known how periodontopathic bacteria colonize in the oral cavity during childhood. the purpose of this study was to investigate the distribution of periodontopathic bacteria in oral cavities of children and their parents and the relationship between the bacterial findings and clinical parameters. | 2004 | 15491344 |
| intra-familial distribution of nine putative periodontopathogens in dental plaque samples analyzed by pcr. | it is of great importance to understand the distribution of periodontopathogens within family members when considering the risk of periodontitis in children. the purpose of this study was to investigate the distribution of periodontopathogens among family members. we used the polymerase chain reaction method to test 4,8, and 7 probands with healthy gingiva, gingivitis, and periodontitis, respectively, and their 60 immediate family members. plaque samples were collected from all erupted teeth sit ... | 2004 | 15508747 |
| the microbiological case for adjunctive therapy for periodontitis. | that chronic periodontitis is an infectious disease is now firmly established, and the primary role of porphyromonas gingivalis, tannerella forsythensis and treponema denticola is generally accepted. treatment by mechanical means such as scaling and root planing or surgery generally results in significant clinical improvement but may not arrest the progress or recurrence of disease. several studies have shown that the probability of achieving lasting stability as measured by the arrest of progre ... | 2004 | 15536782 |
| identification of early microbial colonizers in human dental biofilm. | to elucidate the first colonizers within in vivo dental biofilm and to establish potential population shifts that occur during the early phases of biofilm formation. | 2004 | 15546422 |
| herpesviruses, the missing link between gingivitis and periodontitis? | herpesviruses appear to assume a major etiopathogenic role in various types of destructive periodontal disease. human cytomegalovirus (hcmv), epstein-barr virus (ebv) and hcmv-ebv co-infection are closely associated with disease-active periodontitis in juveniles and adults, with acute necrotizing ulcerative gingivitis in children, and with periodontal abscesses. in particular, hcmv reactivation in periodontitis lesions seems to be linked to advancing disease. hcmv infects periodontal monocytes/m ... | 2004 | 15553977 |
| implant therapy following liver transplantation: clinical and microbiological results after 10 years. | the number of immunocompromised patients is increasing as a consequence of successful organ transplantation. placing dental implants in these patients has been questioned because of their increased risk for infections. the 10-year follow-up data of a 71-year-old liver transplant recipient with long-term immunosuppressive therapy is reported. six months after liver transplantation, two interforaminal implants were inserted in the edentulous mandible, and an overdenture using non-rigid telescopic ... | 2004 | 15295960 |
| microbiological shifts in intra- and extraoral habitats following mechanical periodontal therapy. | the aim of the present study was to analyze the intra- and extraoral colonization dynamics of periodontal pathogens following supra- and subgingival debridement. | 2004 | 15312101 |
| initial antimicrobial effect of controlled-release doxycycline in subgingival sites. | to determine the ability of a 10% doxycycline hyclate controlled-release polymer (atridox) to suppress periodontopathic bacteria when placed subgingivally following scaling and root planing (sc/rp). | 2004 | 15324352 |
| microbiological profile of early onset/aggressive periodontitis patients. | the objectives of this study were to characterize the bacterial profile and to seek possible bacterial associations in the subgingival microbiota of early onset periodontitis/aggressive periodontitis patients by using two different techniques, culture and immunofluorescence. | 2004 | 15327644 |
| selected endodontic pathogens in the apical third of infected root canals: a molecular investigation. | bacteria located at the apical portion of the root canals are conceivably in a strategic position to induce damage to the periradicular tissues and resulting inflammatory diseases. this study sought to investigate the prevalence of 11 selected putative endodontic pathogens in the apical third of infected root canals associated with periradicular lesions. the apical root portion of 23 extracted teeth with carious pulpal exposures and attached periradicular lesions was sectioned, and the root cana ... | 2004 | 15329567 |
| detection of dna from periodontal pathogenic bacteria in biofilm obtained from waterlines in dental units. | direct person-to-person transmission of periodontal bacteria through saliva has recently been widely reported and dental units have been demonstrated to retract saliva from patients under treatment and to release it into the mouths of subjects undergoing the next operation. in this study the presence of a group of periodontal pathogenic bacteria inside waterlines in dental units was investigated using polymerase chain reaction (pcr) based methods. briefly, 18 dental units of three different manu ... | 2004 | 15646054 |
| the strict anaerobe bacteroides fragilis grows in and benefits from nanomolar concentrations of oxygen. | strict anaerobes cannot grow in the presence of greater than 5 micro m dissolved oxygen. despite this growth inhibition, many strict anaerobes of the bacteroides class of eubacteria can survive in oxygenated environments until the partial pressure of o2 (po2) is sufficiently reduced. for example, the periodontal pathogens porphyromonas gingivalis and tannerella forsythensis colonize subgingival plaques of mammals, whereas several other bacteroides species colonize the gastrointestinal tract of a ... | 2004 | 14749831 |
| human salivary function in relation to the prevalence of tannerella forsythensis and other periodontal pathogens in early supragingival biofilm. | previously, we screened 149 subjects and established four groups high or low for salivary killing of oral bacteria, and for aggregation and live and dead adherence of oral bacteria (as a combined factor). caries scores were significantly lower in both high aggregation-adherence groups. subsequently, we found that supragingival total biofilm dna, total streptococci and two major streptococcal rrna variants also were significantly lower in the high aggregation-adherence groups. in this study, we l ... | 2004 | 15126134 |
| [separation of bacteroides forsythus atcc43037 proteins by horizontal two-dimensional gel electrophoresis]. | to set up a rapid, efficient, reliable and accurate method for separation of bacteriodes forsythus proteins. | 2004 | 15293456 |
| persistent colonization with tannerella forsythensis and loss of attachment in adolescents. | colonization with tannerella forsythensis may characterize the conversion of periodontally healthy sites into diseased sites. this three-year study describes the prevalence of t. forsythensis and its relationship to clinical loss of attachment (loa) in a group of adolescents considered at risk of developing early chronic periodontitis. adolescents with (loa+) and without (loa-) loss of attachment were examined at baseline and 1.5 and 3 yrs subsequently. on each occasion, attachment loss was meas ... | 2004 | 14981125 |
| comparison of systemic flurithromycin therapy and clinical procedures in the treatment of periodontal diseases. | the purpose of the present investigation was to evaluate, in 20 periodontal patients, the microbial and clinical effects of flurithromycin therapy plus scaling and root planning (srp) in comparison with srp alone. clinical assessments of plaque, bleeding on probing and pocket depth were made prior to srp alone and srp plus flurithromycin therapy (375 mg twice daily for 5 days) and after both treatments. subgingival plaque samples (n. 180) were taken prior to and after both treatments and analyze ... | 2004 | 15216949 |
| symbionts of the gut flagellate staurojoenina sp. from neotermes cubanus represent a novel, termite-associated lineage of bacteroidales: description of 'candidatus vestibaculum illigatum'. | the symbioses between cellulose-degrading flagellates and bacteria are one of the most fascinating phenomena in the complex micro-ecosystem found in the hindgut of lower termites. however, little is known about the identity of the symbionts. one example is the epibiotic bacteria colonizing the surface of hypermastigote protists of the genus staurojoenina. by using scanning electron microscopy, it was shown that the whole surface of staurojoenina sp. from the termite neotermes cubanus is densely ... | 2004 | 15256565 |
| clinical and microbiologic changes after removal of orthodontic appliances. | the goal of this study was to evaluate the clinical and microbiological factors associated with orthodontic appliances during an episode of gingival inflammation and the impact of appliance removal on periodontal health. this prospective study included 10 patients, aged 12 to 20 years, with clinical signs of gingival inflammation at the final phase of orthodontic treatment (appliance removal). plaque index, gingival index, and probing depth were evaluated, and microbiological samples were collec ... | 2004 | 15356501 |
| specific antibiotics in the treatment of periodontitis--a proposed strategy. | the purpose of the present study was to propose a strategy for the selection of antibiotics that specifically target complexes of periodontal pathogens present in patients with periodontitis. | 2004 | 15025229 |
| gene expression signatures in chronic and aggressive periodontitis: a pilot study. | this pilot study examined gene expression signatures in pathological gingival tissues of subjects with chronic or aggressive periodontitis, and explored whether new subclasses of periodontitis can be identified based on gene expression profiles. a total of 14 patients, seven with chronic and seven with aggressive periodontitis, were examined with respect to clinical periodontal status, composition of subgingival bacterial plaque assessed by checkerboard hybridizations, and levels of serum igg an ... | 2004 | 15154918 |
| periodontal pathogens in atheromatous plaques. a controlled clinical and laboratory trial. | a possible relationship between periodontitis and cardiovascular disease has been suggested. the aims of this controlled clinical study were: (i) to ascertain the presence of periodontal bacteria dna [actinobacillus actinomycetemcomitans, fusobacterium nucleatum, porphyromonas gingivalis, prevotella intermedia, tannerella forsythensis (formerly bacteroides forsythus)] in carotid atheromatous plaques and (ii) to assess the concomitant presence of the same periodontal bacteria dna, if any, in peri ... | 2004 | 15491349 |
| methods of detection of actinobacillus actinomycetemcomitans, porphyromonas gingivalis and tannerella forsythensis in periodontal microbiology, with special emphasis on advanced molecular techniques: a review. | certain specific bacterial species from the subgingival biofilm have demonstrated aetiological relevance in the initiation and progression of periodontitis. among all the bacteria studied, three have shown the highest association with destructive periodontal diseases: actinobacillus actinomycetemcomitans (aa), porphyromonas gingivalis (pg) and tannerella forsythensis (tf). therefore, the relevance of having accurate microbiological diagnostic techniques for their identification and quantificatio ... | 2004 | 15560803 |
| glucose toxicity effect and accumulation of methylglyoxal by the periodontal anaerobe bacteroides forsythus. | growth of the periodontal pathogen bacteroides forsythus in broth cultures showed inhibition in the presence of 10mm glucose added to the medium. glucose inhibition in a number of rumen bacteria has been attributed to the accumulation of methylglyoxal (mg), a highly reactive electrophile known to exhibit cytotoxic effects. hplc analysis revealed elevated concentrations of mg in cultures of seven strains of b. forsythus. mg rose during growth to a maximum at the time of growth inhibition. maximum ... | 2004 | 16701497 |
| prevalence of periodontal pathogens in dental plaque of children. | porphyromonas gingivalis, actinobacillus actinomycetemcomitans, and tannerella forsythensis have been implicated as the main etiological agents of periodontal disease. the purpose of this work was to estimate the prevalence of these organisms in plaque from children without gingivitis (group 1; n = 65) and from those with gingivitis (group 2; n = 53). extracted dna from plaque was subjected to two rounds of pcr targeting the 16s rrna gene using both universal primers and species-specific primers ... | 2004 | 15365002 |
| quantitative real-time polymerase chain reaction based on single copy gene sequence for detection of periodontal pathogens. | to develop a method for quantification of actinobacillus actinomycetemcomitans (aa), porphyromonas gingivalis (pg) and tannerella forsythensis (tf) from subgingival plaque samples based on taqman real-time polymerase chain reaction (pcr) technology. | 2004 | 15560805 |
| quantitative real-time polymerase chain reaction versus culture: a comparison between two methods for the detection and quantification of actinobacillus actinomycetemcomitans, porphyromonas gingivalis and tannerella forsythensis in subgingival plaque samples. | the purpose of this investigation was to validate a real-time quantitative polymerase chain reaction (pcr) assay in identifying and quantifying actinobacillus actinomycetemcomitans, porphyromonas gingivalis and tannerella forsythensis from subgingival plaque samples taken from subjects with different periodontal conditions, when compared with conventional cultural procedures. | 2004 | 15560806 |
| culture-independent identification of periodontitis-associated porphyromonas and tannerella populations by targeted molecular analysis. | periodontitis is the commonest bacterial disease of humans and is the major cause of adult tooth loss. about half of the oral microflora is unculturable; and 16s rrna pcr, cloning, and sequencing techniques have demonstrated the high level of species richness of the oral microflora. in the present study, a pcr primer set specific for the genera porphyromonas and tannerella was designed and used to analyze the bacterial populations in subgingival plaque samples from inflamed shallow and deep site ... | 2004 | 15583276 |
| the aetiology and treatment of oral halitosis: an update. | halitosis refers to the condition of offensive mouth odour. more than 90% of cases of halitosis originate from the oral cavity. the implicated bacteria (fusobacterium nucleatum, prevotella intermedia, and tannerella forsythensis) are located in stagnant areas in the oral cavity, such as the dorsal surface of tongue, periodontal pockets, and interproximal areas. these bacteria proteolyse the amino acids releasing volatile sulphur compounds. the management of halitosis involves determining and eli ... | 2004 | 15591601 |
| microbiological changes with the use of locally delivered doxycycline in the periodontal treatment of smokers. | the aim of this study was to evaluate the effect of the association of locally delivered doxycycline 10% and scaling and root planing in the subgingival plaque of smokers. | 2004 | 15732860 |
| immunohistological analysis of tannerella forsythia-induced lesions in a murine model. | tannerella forsythia has been implicated as a defined periodontal pathogen. in the present study a mouse model was used to determine the phenotype of leukocytes in the lesions induced by subcutaneous injections of either live (group a) or nonviable (group b) t. forsythia. control mice (group c) received the vehicle only. lesions were excised at days 1, 2, 4, and 7. an avidin-biotin immunoperoxidase method was used to stain infiltrating cd4+ and cd8+ t cells, cd14+ macrophages, cd19+ b cells, and ... | 2005 | 15612941 |
| [association between bacteroides forsythus in the infected root canals and clinical symptoms of chronic apical periodontitis]. | to investigate the distribution of bacteroides forsythus in root canals with chronic apical periodontitis and to determine its associations with clinical symptoms. | 2005 | 15702778 |
| the prevalence and pathogenic differences of porphyromonas gingivalis fima genotypes in patients with aggressive periodontitis. | the fima gene, which encodes fimbrillin (fima), is found in porphyromonas gingivalis and has been classified into six genotypes based on nucleotide sequence. p. gingivalis that possesses the type ii fima gene is prevalent in adult periodontitis. | 2005 | 15733149 |
| combined effects of nd:yag laser irradiation with local antibiotic application into periodontal pockets. | the purpose of the present study was to assess the effects of nd:yag laser irradiation into periodontal pockets with or without the combination of local antibiotic application on clinical parameters and microbiological prevalence. sixteen patients, each of whom had 4 or more sites with probing depth e x 4mm were included in this study. they were monitored clinically and microbiologically at baseline, 1 and 3 months after the treatment. subgingival plaque samples were taken from periodontally inv ... | 2005 | 15736890 |
| effect of khat chewing on 14 selected periodontal bacteria in sub- and supragingival plaque of a young male population. | the habit of chewing khat (catha edulis) for its amphetamine-like effects is highly prevalent in yemen and east africa, and has expanded to western countries. the purpose of this study was to estimate and compare the prevalence and levels of 14 periodontal bacteria in gingival plaque of khat chewers and khat nonchewers, as well as of khat chewing sides and khat nonchewing sides. | 2005 | 15836514 |
| differences in the subgingival microbiota of swedish and usa subjects who were periodontally healthy or exhibited minimal periodontal disease. | previous studies have shown differences in the mean proportions of subgingival species in samples from periodontitis subjects in different countries, which may relate to differences in diet, genetics, disease susceptibility and manifestation. the purpose of the present investigation was to determine whether there were differences in the subgingival microbiotas of swedish and american subjects who exhibited periodontal health or minimal periodontal disease. | 2005 | 15642056 |
| detection of tannerella forsythia and/or prevotella intermedia might be useful for microbial predictive markers for the outcome of initial periodontal treatment in koreans. | a proportion of diseased sites in periodontal disease do not respond to the initial treatment, which might be due in part to the presence of specific microbial pathogens. the aim of this study was to clarify the value of microbial screening for predicting the outcome of periodontal treatment in koreans using a polymerase chain reaction (pcr). this study enrolled 32 adults with periodontal disease. microbial and clinical examinations were performed at the baseline and after the initial treatment ... | 2005 | 15665448 |
| microbiological and clinical outcomes and patient satisfaction for two treatment options in the edentulous lower jaw after 10 years of function. | long-term data on microbiological and clinical outcome as well as on patient satisfaction after implant therapy in the edentulous mandible are limited. especially comparisons between fixed full prostheses (ffps) and overdentures (ods), or between anchoring systems for the latter are scarce. | 2005 | 15877747 |
| 16s-23s rrna gene internal transcribed spacer sequences for analysis of the phylogenetic relationships among species of the genus porphyromonas. | the 16s-23s rrna gene internal transcribed spacer (its) regions of 11 reference strains of porphyromonas species, together with bacteroides distasonis and tannerella forsythensis, were analysed to examine interspecies relationships. compared with the phylogenetic tree generated using 16s rrna gene sequences, the resolution of the its sequence-based tree was higher, but species positioning and clustering were similar with both approaches. the recent separation of porphyromonas gulae and porphyrom ... | 2005 | 15774632 |
| detection of periodontopathic bacteria and an oxidative stress marker in saliva from periodontitis patients. | we assessed the salivary levels of periodontopathic bacteria and 8-hydroxydeoxyguanosine (8-ohdg) in patients with periodontitis. the salivary levels of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and tannerella forsythia (formerly bacteroides forsythus) were assessed using real-time polymerase chain reaction. the 8-ohdg levels were determined using an enzyme-linked immunosorbent assay. the salivary levels of 8-ohdg, p. gingivalis, and t. forsythia in the periodontitis patien ... | 2005 | 15943765 |
| contact-dependent regulation of a tannerella forsythia virulence factor, bspa, in biofilms. | tannerella forsythia is one of the periodontal organisms implicated in the development of periodontal diseases. the surface associated and secreted protein, bspa (encoded by the bspa gene), of this bacterium is an important virulence factor. the present study was carried out to examine the regulation of the bspa gene during biofilm growth and contact stimuli encountered in interbacterial interactions. the expression levels of the bspa transcript were determined by real-time rt-pcr approach. the ... | 2005 | 16006067 |
| serum antibodies to oral anaerobic bacteria in patients with rheumatoid arthritis. | this study was conducted to determine the component that causes the disease in rheumatoid arthritis (ra), which shows great resemblance to periodontitis in a pathologic context. | 2005 | 16369381 |
| [efficacy variation of erythromycin and spiramycin on periopathogens in aggressive periodontitis. an in vitro comparative study]. | erythromycin (ery) and spiramycin (spi) are the most frequently prescribed macrolides by dentists. however, the emergence of resistant anaerobic subgingival bacteria imposes an increased vigilance. this study aims to compare these macrolides efficacy on principal periopathogens. | 2005 | 16087299 |
| detection of bacteria in endodontic samples by polymerase chain reaction assays and association with defined clinical signs in italian patients. | the presence of selected bacteria (enterococcus faecalis, porphyromonas gingivalis, prevotella intermedia, tannerella forsythensis, treponema denticola) in infected root canals was studied using polymerase chain reaction (pcr) assays, and the association of bacteria with clinical signs of endodontic disease was assessed. the null hypothesis, that no difference could be observed between clinical signs of apical periodontitis and a specific bacterial strain, was tested. | 2005 | 16101964 |
| cross-reactivity of groel antibodies with human heat shock protein 60 and quantification of pathogens in atherosclerosis. | chronic infections such as those caused by chlamydia pneumoniae and periodontopathic bacteria such as porphyromonas gingivalis have been associated with atherosclerosis, possibly due to cross-reactivity of the immune response to bacterial groel with human heat shock protein (hhsp) 60. | 2005 | 16101965 |
| prevalence of putative periodontopathogens in subgingival dental plaques from gingivitis lesions in korean orthodontic patients. | the objective of this study was to detect and compare the presence of periodontopathogens in the subgingival plaques of gingivitis lesions in adults who wore fixed orthodontic appliances, as opposed to adults who did not wear any orthodontic appliances. thirty-six individuals participated in this study. nineteen of these subjects did not wear any orthodontic appliances, and these subjects comprised the control group. the other 17 individuals had been wearing fixed orthodontic appliances for at l ... | 2005 | 15995644 |
| actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and tannerella forsythensis are components of a polymicrobial intracellular flora within human buccal cells. | previously, we used in situ hybridization and confocal microscopy to detect the periodontal pathogens actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and tannerella forsythensis within buccal epithelial cells taken directly from the mouth. this study tested the hypothesis that the intracellular flora of buccal cells is polymicrobial. mixtures containing a red fluorescent universal probe paired with green fluorescent versions of either a. actinomycetemcomitans-, p. gingivalis-, or ... | 2005 | 15615877 |
| oral bacteria in the occluded arteries of patients with buerger disease. | recent studies have suggested that infectious organisms play a role in vascular diseases. in this study, to explore a possible link between oral infection and buerger disease, we investigated whether oral (periodontal) bacteria were present in occluded arteries removed from patients with characteristic buerger disease. | 2005 | 16012459 |
| the effect of haart on salivary microbiota in the women's interagency hiv study (wihs). | study the prevalence of potentially pathogenic microorganisms in saliva of hiv-positive women in the women's interagency hiv study. | 2005 | 16301151 |
| the distribution of tannerella forsythia in an adolescent and adult population. | the fact that tannerella forsythia, an important periopathogen, is difficult to cultivate from mixed infections has impeded precise estimates of its distribution within a given population. in order to discern t. forsythia alone from the mixed infection of plaque, the use of sensitive 16s ribosomal rna based polymerase chain reaction (pcr) detection is necessary. | 2005 | 16302927 |
| streptococci dominate the diverse flora within buccal cells. | previously, we reported that intracellular actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and tannerella forsythensis were present within buccal epithelial cells from human subjects, as lesser components of a polymicrobial flora. in this study, we further characterized that intracellular flora by using the same double-labeling techniques to identify fusobacterium nucleatum, prevotella intermedia, oral campylobacter species, eikenella corrodens, treponema denticola, gemella haemo ... | 2005 | 16304448 |
| cytomegalovirus-associated periodontitis and guillain-barré syndrome. | guillain-barré syndrome (gbs), an autoimmune disorder of the peripheral nervous system, is characterized by rapidly ascending neural paralysis, hyporeflexia, and areflexia. the polyneuropathy of the gbs affects one to four humans per 100,000 of the population annually throughout the world (adults and children). the pathogenesis of gbs remains unclear. however, there are increasing indications that the disease is triggered by a preceding well-established febrile infection by cytomegalovirus (cmv) ... | 2005 | 16332244 |
| stimulation of growth of porphyromonas gingivalis by cell extracts from tannerella forsythia. | in order to examine if tannerella forsythia stimulates the growth of porphyromonas gingivalis, an in vitro study was performed. background: p. gingivalis and t. forsythia are often isolated simultaneously from active periodontitis sites, indicating that these bacteria somewhat interact in the periodontal environment. we reported previously that mixed infection of p. gingivalis and t. forsythia synergistically induced lesion formation in a murine abscess model, and gingipains of p. gingivalis pla ... | 2005 | 15733144 |
| tobacco smoking and periodontal microflora in a saudi arabian population. | to explore the subgingival periodontal microflora in a saudi arabian population with a special focus on its relationship with various smoking habits. | 2005 | 15882210 |
| proportional distribution of the red complex and its individual pathogens after sample storage using the checkerboard dna-dna hybridization technique. | information on the impact of sample storage prior to analysis by dna methods is limited. | 2005 | 15882222 |
| clinical and microbiological evaluation of the effectiveness of the nd:yap laser for the initial treatment of adult periodontitis. a randomized controlled study. | enhancement of the results obtained by scaling and planing is most often sought by using antimicrobial therapies. laser beams have been shown to be bactericidal and could possibly target pathogens more effectively and with fewer compliance problems than antiseptic solutions. | 2005 | 15882229 |
| identification of periodontal pathogens in atherosclerotic vessels. | epidemiological studies have shown that periodontitis may be associated with presence of atherosclerosis. dna from periodontal pathogens has been detected in atherosclerotic lesions, but viable oral bacteria have not yet been isolated from atherosclerotic plaques. the purpose of the present study was to determine if viable oral bacteria could be isolated from atherosclerotic lesions and if dna from periodontal pathogens could be detected by use of polymerase chain reaction (pcr) techniques. | 2005 | 15898933 |
| characteristics of periodontal microflora in acute myocardial infarction. | periodontitis has been linked to increased risk of cardiovascular diseases. systemic reactions associated with cardiovascular events may depend on characteristics of the subgingival microflora in periodontitis. our objectives were to compare the numbers of cultivable bacteria, composition of subgingival microflora and clonal distribution of actinobacillus actinomycetemcomitans (a. actinomycetemcomitans) in two groups of patients with generalized chronic periodontitis (gcp), one with an acute myo ... | 2005 | 15898935 |
| adjunctive antimicrobial therapy of periodontitis: long-term effects on disease progression and oral colonization. | this study assessed the long-term effects of adjunctive antimicrobial therapy on periodontal disease progression and oral colonization. | 2005 | 15898936 |
| debridement and local application of tetracycline in the management of persistent periodontitis. clinical and microbiological results after 12 months. | the purpose of this study was to evaluate the clinical and microbiological efficacy of local delivery of tetracycline in persistent periodontal lesions after topical therapy. | 2005 | 15902062 |
| periodontal pathogens: a quantitative comparison of anaerobic culture and real-time pcr. | periodontitis is a multi-factorial chronic inflammatory and destructive disease of the tooth-supporting tissues. quantitative anaerobic culture techniques have been used for microbial diagnosis of the different forms of the disease. the aim of this study was to compare real-time pcr with quantitative anaerobic culture for detection and quantification of 5 prominent periodontal pathogens. real-time pcr assays with the 16s rrna genes of actinobacillus actinomycetemcomitans, prevotella intermedia, ... | 2005 | 15919188 |
| synergy between tannerella forsythia and fusobacterium nucleatum in biofilm formation. | during dental plaque formation, the interaction of different organisms is important in the development of complex communities. fusobacterium nucleatum is considered a 'bridge-organism' that facilitates colonization of other bacteria by coaggregation-mediated mechanisms and possibly by making the environment conducive for oxygen intolerant anaerobes. these studies were carried out to determine whether coaggregation between f. nucleatum and tannerella forsythia is important in the formation of mix ... | 2005 | 15612944 |
| antimicrobial susceptibility variation of 50 anaerobic periopathogens in aggressive periodontitis: an interindividual variability study. | the frequent use of antibiotics in developed countries has led to the emergence of widespread bacterial resistance. in this study, the interindividual variability of the antibiotic susceptibility of 50 putative microorganisms in aggressive periodontitis patients has been evaluated by means of vc (variation coefficient). | 2005 | 15943770 |
| microarrays complement culture methods for identification of bacteria in endodontic infections. | the aim of this study was to investigate the microbial composition of necrotic root canals using culture methods and microarray technology. twenty uniradicular teeth with radiographic evidence of periapical bone loss and with no previous endodontic treatment were selected for this study. for molecular diagnosis a dna chip with 20 different species-specific, 16s-rdna-directed catcher probes was used. the microorganisms most frequently detected by the dna chip were: micromonas micros, fusobacteriu ... | 2005 | 15943771 |
| predictive value of clinical and microbiological parameters for the treatment outcome of scaling and root planing. | to compare the clinical and microbiological outcome of non-surgical periodontal therapy after 6 months with data obtained after hygienic phase or 6 weeks after completion of non-surgical therapy, in order to evaluate the value of clinical and microbiological parameters to predict treatment success. | 2005 | 15966873 |
| comparison of culture and real-time pcr for detection and quantification of five putative periodontopathogenic bacteria in subgingival plaque samples. | bacterial cultivation is a well-established method for analyzing plaque samples. real-time polymerase chain reaction (pcr) is a novel rapid method for the identification and quantification of periodontopathogenic bacteria that has been recently introduced. in this study, we compared real-time pcr with conventional anaerobic cultivation. | 2005 | 15966886 |
| severe periodontitis in a 5-year-old girl with hyperimmunoglobulin e syndrome. | the hyperimmunoglobulin e syndrome (hies) is a multisystem disorder that affects the: (1) dentition; (2) skeleton; (3) connective tissues; and (4) immune system. little is known about periodontal manifestations of the syndrome. the purpose of this report was to describe a 5-year-old girl with suspected autosomal-recessive hies syndrome who revealed profusely bleeding and painful gingiva and generalized aggressive periodontitis. a polymerase chain reaction (pcr)-based microbiological examination ... | 2005 | 15839398 |
| tannerella forsythia-induced alveolar bone loss in mice involves leucine-rich-repeat bspa protein. | tannerella forsythia (formerly bacteroides forsythus) is one of the periodontal pathogens recently implicated in the development of periodontal disease. the cell-surface-associated, as well as the secreted, leucine-rich-repeat protein (bspa) of this bacterium have been suggested to play roles in bacterial adherence, and also in inflammation, by triggering release of pro-inflammatory cytokines from monocytes and chemokines from osteoblasts, leading to inflammation and bone resorption. in this stu ... | 2005 | 15840784 |
| porphyromonas gingivalis, treponema denticola, and tannerella forsythia: the "red complex", a prototype polybacterial pathogenic consortium in periodontitis. | 2005 | 15853938 | |
| molecular analysis of human oral microbiota. | the application of molecular, mainly 16s ribosomal rna (rrna)-based approaches enables researchers to bypass the cultivation step and has proven its usefulness in studying the microbial composition in a variety of ecosystems, including the human oral cavity. in this mini-review, we describe the impact of these culture-independent approaches on our knowledge of the ecology of the human oral cavity and provide directions for future studies that should emphasize the role of specific strains, specie ... | 2005 | 15853975 |
| detection of actinobacillus actinomycetemcomitans but not bacteria of the red complex in aortic aneurysms by multiplex polymerase chain reaction. | aortic aneurysms affect an increasing number of elderly patients and cause considerable morbidity and mortality. the understanding of the mechanisms involved in the pathogenesis of aortic aneurysms is unclear and little is known about the role of microorganisms in the development of the condition. the aim of the present study was to examine aortic aneurysm samples for the presence of four putative periodontal pathogens: actinobacillus actinomycetemcomitans, treponema denticola, tannerella forsyt ... | 2005 | 15857100 |
| loop-mediated isothermal amplification method for rapid detection of the periodontopathic bacteria porphyromonas gingivalis, tannerella forsythia, and treponema denticola. | loop-mediated isothermal amplification (lamp), a novel nucleic acid amplification method, was developed for the rapid detection of the major periodontal pathogens porphyromonas gingivalis, tannerella forsythia, and treponema denticola. the lamp method amplifies dna with high specificity, efficiency, and rapidity under isothermal conditions using a set of four specially designed primers and a dna polymerase with strand displacement activity. in this study, we initially designed the primers for la ... | 2005 | 15872275 |
| mechanisms of porphyromonas gingivalis-induced monocyte chemoattractant protein-1 expression in endothelial cells. | monocyte chemoattractant protein-1 (mcp-1) is expressed in vascular endothelial cells of inflamed gingival tissues and plays an important role in periodontal pathogenesis. endothelial cells produce high levels of mcp-1 in response to porphyromonas gingivalis, an important periodontal pathogen. the present study investigated the mechanisms involved in mcp-1 production by human umbilical vein endothelial cells (huvec) following infection with p. gingivalis. in contrast to p. gingivalis, bacteroide ... | 2005 | 15780578 |
| scaling and root planing, systemic metronidazole and professional plaque removal in the treatment of chronic periodontitis in a brazilian population ii--microbiological results. | the current investigation evaluated changes in levels and proportions of 39 bacterial species in subgingival plaque samples after scaling and root planing (srp) alone or in combination with systemic metronidazole and/or professional cleaning in subjects with chronic periodontitis. | 2005 | 15811059 |
| differences in prevalence of selected bacterial species in primary endodontic infections from two distinct geographic locations. | this study intended to compare the prevalence of 7 putative endodontic pathogens in samples of primary endodontic infections taken from patients of 2 distant geographic locations. | 2005 | 15829891 |
| non-surgical periodontal therapy with adjunctive topical doxycycline: a double-masked, randomized, controlled multicenter study. ii. microbiological results. | topical application of active substances offers an additional option in periodontal therapy. the aim of this study was to examine the influence of the administration of a novel, biodegradable 14% doxycycline gel on microbiological findings, in connection with scaling and root planing. | 2005 | 15830639 |