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effect of cations on effective permeability of leaf cuticles to sulfuric acid.many plants are exposed to prolonged episodes of anthropogenic acid precipitation with ph values of 4 or less, but there is little evidence of widespread direct damage to the plant cells. acids appear to permeate leaf cuticle via charged pores, which act as a fixed buffer that delays but does not stop acid movement. we investigated the effect of cations on the movement of protons through astomatous isolated leaf cuticles of pear (pyrus communis l.) and rough lemon (citrus limon [l.] burm. fils c ...199312231931
patterns of effective permeability of leaf cuticles to acids.plants in the field are frequently exposed to anthropogenic acid precipitation with ph values of 4 and below. for the acid to directly affect leaf tissues, it must pass through the leaf cuticle, but little is known about the permeability of cuticles to protons, or about the effect of different anions on this permeability. we investigated the movement of protons through isolated astomatous leaf cuticles of grapefruit (citrus x paradisi macfady.), rough lemon (citrus limon [l.] burm. fils cv ponde ...199312231681
two methods of haploidization in pear, pyrus communis l.: greenhouse seedling selection and in situ parthenogenesis induced by irradiated pollen.seedlings of 12 crosses involving pear varieties or hybrids were observed for the presence of haploid plants. on the basis of phenotypic characteristics, 17 plants corresponded to the haploid condition and, of these, 12 were determined by chromosome counting to be haploid (2n=x=17). in addition, and in order to induce in situ parthenogenesis, several pear varieties were pollinated with a selected clone carrying a homozygous dominant marker gene for the colour of red. this pollen had previously b ...199324190217
[inflammatory gorham disease].the case of a 48-year old female with massive osteolysis since the age of 13 years is reported. she had inflammatory flares which resolved spontaneously or after a transient discharge of lymph.19938012338
kasabach-merritt syndrome with bone involvement: the pseudomalignant sign of gorham. 19938315070
pregnancy complicated by gorham disease.a pregnancy with gorham disease (massive osteolysis syndrome) is described. to our knowledge, this is the first reported case of a pregnancy associated with this disease.19938469480
molecular characterization of a polygalacturonase inhibitor from pyrus communis l. cv bartlett.a polygalacturonase inhibitor glycoprotein with an apparent molecular mass of 43 kd was purified from pear (pyrus communis l. cv bartlett) fruit. chemical deglycosylation of this protein decreased the molecular mass to 34 kd. gas chromatographic analysis suggests that n-linked glycosylation accounts for the majority of sugar moieties. partial amino acid sequence analysis of the purified polygalacturonase inhibitor protein provided information used to amplify a corresponding cdna by polymerase ch ...19938108494
[clinico-pathological and orthopedic-surgical aspects of essential osteolysis (gorham disease)].authors present two cases. one of their patient was followed for 30 years. they discuss the pathological characters and diagnostic difficulties of the disease. they have observed that with radical operations of--according to necessity--serial surgical interventions the outcome of patients can be properly controlled against recent pessimistic opinions.19938464630
[validity of the brief psychiatric rating scale].the internal structure and validity of terms used in the brief psychiatric rating scale (overall and gorham, 1962) was analyzed. the data which had been gained at the entrance examination of 1557 inpatients with the diagnosis according to icd-9 (schizophrenia simplex, schizophrenia paranoids, schizoaffective disease depressive type, schizoaffective disease manic type, manic-depressive disease manic phase, manic-depressive disease depressive phase) were processed using stepwise discriminant analy ...19947882071
molecular cloning of a gene encoding an arabinogalactan-protein from pear (pyrus communis) cell suspension culture.arabinogalactan-proteins (agps) are proteoglycans containing a high proportion of carbohydrate (typically > 90%) linked to a protein backbone rich in hydroxyproline (hyp), ala, ser, and thr. they are widely distributed in plants and may play a role in development. the structure of the carbohydrate of some agps is known in detail but information regarding the protein backbone is restricted to a few peptide sequences. here we report isolation and partial amino acid sequencing of the protein backbo ...19947937945
gorham disease of the spine.this is a case report of gorham disease of the spine with review of the literature.19947809757
concurrent validity of the cognitive component of schizophrenia: relationship of panss scores to neuropsychological assessments.cognitive symptoms of schizophrenia may represent a separate component of the disorder that is distinct from positive or negative syndromes. in a previous study, we reported a factor analysis of the positive and negative syndrome scale (panss) that revealed five components, one of which we labeled the cognitive component. in the present study, we explored the validity of the panss cognitive component by examining correlations between neuropsychological measures and the five factor-analytically d ...19947701028
protein synthesis and breakdown during heat shock of cultured pear (pyrus communis l.) cells.cultured pear (pyrus communis l. cv passe crassane) cells were subjected to temperatures of 39, 42, and 45[deg]c. heat-shock protein (hsp) synthesis was greater at 30[deg]c than at temperatures above 40[deg]c and continued for up to 8 h. both cellular uptake of radiolabeled methionine and total protein synthesis were progressively lower as the temperature was increased. polysome levels decreased immediately when cells were placed at 39 or 42[deg]c, although at 39[deg]c the levels began to recove ...199412232178
isolation and characterization of rhamnogalacturonan oligomers, liberated during degradation of pectic hairy regions by rhamnogalacturonase.digests of modified hairy regions of apple pectin (mhr) obtained after degradation by rhamnogalacturonase (rgase) were analyzed for oligomer composition using high-performance anion-exchange chromatography and pulsed amperometric detection. a series of oligomers which appear to be characteristic of rgase degradation could be recognized. these oligomers were isolated on a preparative scale by size-exclusion chromatography and preparative anion-exchange chromatography and analyzed for sugar compos ...19948194077
nucleotide sequences of apple stem pitting virus and of the coat protein gene of a similar virus from pear associated with vein yellows disease and their relationship with potex- and carlaviruses.the nucleotide sequence (9306 nucleotides) of cdna clones of apple stem pitting virus (aspv) obtained from a double-stranded rna template, extracted from diseased plant tissue, was determined. the genome is composed of five open reading frames (orfs) encoding putative proteins with m(r)s of 247083, 25147, 12832, 7429 and 43712, and has a poly(a) tail. using two oligonucleotides designed from the aspv sequence information a 1598 bp fragment from near the 3' terminus of the viral rna, containing t ...19948021584
production of single-cell oil from prickly-pear juice fermentation by cryptococcus curvatus grown in batch culture.the biomass of cryptococcus curvatus, an oleaginous yeast, reached 11 g/l and accumulated 46% (w/w) lipid when grown for 35 h in batch culture on diluted (25%) prickly-pear juice. the c:n ratio of the juice was about 50 g/g. the efficiency of substrate conversion was 0.48 g/g for biomass and 0.22 g/g for lipids. the extracted lipids were mainly oleic (18:1) and palmitic (16:0) acids and the quality of pipid approached that of palm oil.199424421128
chlorine dioxide for reduction of postharvest pathogen inoculum during handling of tree fruits.alternatives to hypochlorous acid and fungicides are needed for treatment of fruit and fruit-handling facilities. chlorine dioxide was evaluated and found effective against common postharvest decay fungi and against filamentous fungi occurring on fruit packinghouse surfaces. in vitro tests with conidial or sporangiospore suspensions of botrytis cinerea, penicillium expansum, mucor piriformis, and cryptosporiopsis perennans demonstrated >99% spore mortality within 1 min when the fungi were expose ...199416349354
the transcription factor bsap (nf-hb) is essential for immunoglobulin germ-line epsilon transcription.treatment of murine splenic b lymphocytes and certain b-lineage cell lines with mitogen (lps) and the lymphokine il-4 has been shown to induce expression of germ-line epsilon transcripts (l epsilon transcripts) and class switching to the c epsilon gene. three protein complexes, one of which (complex 3) is constitutively expressed, have been shown to bind to a 179-base pair lps/il-4-responsive l epsilon promoter (rothman, p., s. c. li, b. gorham, l. glimcher, f. w. alt, and m. boothby. 1991. mol. ...19948144891
hair cells of different shapes and their placement along the frog crista ampullaris.the list of distinguishing morphological features of hair cells includes: type i and type ii afferent innervation, and length, shapes and arrangements of stereo- and kinocilia. we now add to this list the shapes of the hair cells themselves and their placement within the mechanosensory organ, in this case the semicircular canal. although hair cells of the crista ampullaris of the frog are only of type ii they may now be further classified into three sub-groups according to shape: club-, cigar- a ...19948157499
cytoplasmic forms of fission yeast casein kinase-1 associate primarily with the particulate fraction of the cell.two novel casein kinase-1 homologs, cki1+ and cki2+, have been isolated from schizosaccharomyces pombe and characterized. both genes reside on chromosome ii and encode approximately 50-kda proteins that are related structurally and enzymatically to the yck gene products of budding yeast. subcellular fractionation experiments demonstrate that cki1 and cki2 are both cytoplasmic enzymes that do not overlap in subcellular distribution and that probably play distinct roles within the cell. although g ...19948163505
muscle- and pneumatic-powered counterpulsating lvads: a pilot study.there is a worldwide interest in supporting the failing heart with a skeletal muscle by either wrapping it around the natural heart (dynamic cardiomyoplasty) or by constructing a skeletal muscle ventricle (smv) used for counterpulsation. conventional cardiomyoplasty in many clinics carries an operative mortality rate of 15-20% partly because it requires 6 weeks to train the muscle to contract continually. a flexible, pear-shaped blood pump with an inflatable air chamber was designed and made aro ...19948185491
prickly pear (opuntia sp.) pectin alters hepatic cholesterol metabolism without affecting cholesterol absorption in guinea pigs fed a hypercholesterolemic diet.prickly pear pectin intake decreases plasma ldl concentrations by increasing hepatic apolipoprotein b/e receptor expression in guinea pigs fed a hypercholesterolemic diet. to investigate whether prickly pear pectin has an effect on cholesterol absorption and on enzymes responsible for hepatic cholesterol homeostasis, guinea pigs were fed one of three semipurified diets, each containing 15 g lard/100 g diet: 1) the lard-basal diet with no added cholesterol or prickly pear pectin (lb diet); 2) the ...19948207539
maturation of somatostatin immunoreactivity in the pigeon retina: morphological characterization and quantitative analysis.in addition to a modulatory function, somatostatin (ss) is likely to exert a morphogenetic and/or trophic role in the developing nervous system. in this study, a mouse monoclonal antibody directed to ss was used to investigate the posthatching development of ss-immunoreactivity (ss-ir) in the pigeon retina to provide a basis for a better understanding of the role of this peptide in retinal maturation. in the adult, ss-ir was observed in amacrine cells located in the inner nuclear layer (inl) of ...19947912104
immunohistochemical localization of neuropeptide y and somatostatin in human fetal retina.the localization of neuropeptide y and somatostatin in the retina of six prenatal human specimens was determined by the utilization of light microscopic immunoperoxidase and immunofluorescence methods. the age of the prenatal fetuses ranged from 15 to 40 weeks of gestation. at 15 weeks, round or pear-shaped neuropeptide y-immunopositive cells were observed in the inner nuclear layer and somatostatin immunoreactivity was only detected in the ganglion cell layer. positive neuropeptide y ganglion c ...19947936198
falciparum malaria in naturally infected human patients: viii. fine structure of intraerythrocytic asexual forms before and during chloroquine treatment.the fine structure of the intraerythrocytic asexual forms of plasmodium falciparum is described before and during treatment with chloroquine (cq) in the venous blood of 27 saudi patients whe were heavily infected with the parasite. before treatment, the "pear" shaped merozoite was rapidly transformed into a ring-shaped trophozoite. this was enclosed in a parasitophorous vacuole bounded by a single membrane derived from the host erythrocyte. the parasite feeding mechanism involves several previou ...19947951397
a three-dimensional reconstruction study of the rough er-golgi interface in serial thin sections of the pancreatic acinar cell of the rat.distinctive views of the tubulo-vesicular elements interposed between the endoplasmic reticulum (er) and the golgi apparatus were obtained in thin sections. the tubules that protrude from the transitional rough er (trer) are of dissimilar length. the numbers of tubules and of the nearby omega- and pear-shaped profiles decrease after fasting and are partially restored by refeeding. this formation is designated herein as the budding chamber of the trer. close to the budding chamber, clusters of 56 ...19948006070
clinical features of a previously undescribed codon 216 (proline to serine) mutation in the peripherin/retinal degeneration slow gene in autosomal dominant retinitis pigmentosa.mutations in the human peripherin/retinal degeneration slow (rds) gene have been found in patients with macular dystrophies as well as in those with autosomal dominant retinitis pigmentosa. the authors studied the clinical features in members of two families with autosomal dominant retinitis pigmentosa and a previously unreported mutation in the peripherin/rds gene.19948058286
structure and expression of an inhibitor of fungal polygalacturonases from tomato.a polygalacturonase inhibitor protein (pgip) was characterized from tomato fruit. differential glycosylation of a single polypeptide accounted for heterogeneity in concanavalin a binding and in molecular mass. tomato pgip had a native molecular mass of 35 to 41 kda, a native isoelectric point of 9.0, and a chemically deglycosylated molecular mass of 34 kda, suggesting shared structural similarities with pear fruit pgip. when purified pgips from pear and tomato were compared, tomato pgip was appr ...19948061315
disturbance of alveolar lining layer: effects on alveolar microstructure.to further study the influence of altered surface tensions on alveolar micromechanics, we analyzed the structure-function relationships in excised rabbit lungs filled with or rinsed by a fluorocarbon (approximately 15 mn/m) or by hexadecane (approximately 25 mn/m). the lungs were fixed and dehydrated by vascular perfusion, and the tissue samples were analyzed by light, transmission, and scanning electron microscopy. we made three observations. 1) pressure-volume (p-v) loops hexadecane-filled lun ...19948063660
genetic and plasmid diversity within natural populations of pseudomonas syringae with various exposures to copper and streptomycin bactericides.we examined the genetic and plasmid diversity within natural populations of pseudomonas syringae isolated from three ornamental pear nurseries in eastern oklahoma. the bactericide spray regimen differed at each nursery; copper and streptomycin, only copper, and no bactericides were applied at nurseries i, ii, and iii respectively. resistance to copper (cur) and resistance to streptomycin (smr) were determined for 1,938 isolates of p. syringae; isolates from nurseries i and ii were generally cur ...19947811082
sexual dimorphism and developmental change of the salivary glands in adult culicoides variipennis (diptera: ceratopogonidae).salivary glands of adult male and female culicoides variipennis (coquillett) were sexually dimorphic when examined by phase contrast light microscopy. female salivary glands were larger and more complex than those in males. each female gland consisted of a main gland, which was subdivided into a proximal neck and a distal body with reference to the salivary duct, and four accessory glands. each male salivary gland consisted of a pear-shaped body with a constriction, or neck, that divided it into ...19947815404
genetics of galactose metabolism of erwinia amylovora and its influence on polysaccharide synthesis and virulence of the fire blight pathogen.galactose metabolism mutants of erwinia amylovora were created by transposon insertions and characterized for their growth properties and interaction with plant tissue. the nucleotide sequence of the gale gene was determined. the gene, which encodes udp-galactose 4-epimerase, shows homology to the gale genes of escherichia coli, neisseria gonorrhoeae, rhizobium meliloti, and other gram-negative bacteria. cloned dna with the gale and with the galt and galk genes did not share borders, as judged b ...19947507102
analysis of the high molecular weight rhoptry complex of plasmodium falciparum using monoclonal antibodies.twenty-one monoclonal antibodies, obtained after immunization of mice with erythrocytic stages of plasmodium falciparum, produced a double dot image in ifa. immunoelectronmicroscopy indicated that the mabs reacted with the rhoptries. rhoptries are pear-shaped apical organelles, believed to be involved in invasion of the host cell by the parasite. the mabs all immunoprecipitated the high molecular weight antigen complex. some mabs recognized on immunoblots only 1 protein of this complex, whereas ...19947517517
synaptic connections of central carotid sinus afferents in the nucleus of the tractus solitarius of the rat. ii. connections with substance p-immunoreactive neurons.a combined transganglionic transport and immunocytochemical technique was used to study the synaptic morphology of central carotid sinus afferents and substance p-immunoreactive neurons in the commissural subnucleus of the nucleus of the tractus solitarius in rats. a large population of substance p-immunoreactive neurons (88.32%) were seen in close association with central carotid sinus afferents by light microscopy. however, many labelled central carotid sinus afferents appeared not associated ...19947522269
investigation of sheep-associated malignant catarrhal fever virus infection in ruminants by pcr and competitive inhibition enzyme-linked immunosorbent assay.development of control measures for the gammaherpesviral disease of cattle known as sheep-associated malignant catarrhal fever (sa-mcf) has been hampered by a lack of accurate diagnostic tests either for the causative virus or for antibody against that virus. a recently developed competitive-inhibition enzyme-linked immunosorbent assay (ci-elisa) for the detection of antibody to malignant catarrhal fever (mcf) virus (mcfv) in ruminants based on a monoclonal antibody to a widely conserved epitope ...19957559946
pear blister canker viroid: sequence variability and causal role in pear blister canker disease.the sequences of several cdna clones of pear blister canker viroid (pbcvd) p1914t and p47a isolates have been determined. seven out of eight p1914t clones analysed have a constant sequence which differs at six positions from that of the p2098t isolate reported previously. the remaining p1914t clone (8) has a single nucleotide substitution. the same six changes have been also observed in most of the ten p47a clones sequenced. however, some p47a clones show additional variability in positions on b ...19957595369
genetic analysis of nonpathogenic agrobacterium tumefaciens mutants arising in crown gall tumors.little is known about the effect of the host on the genetic stability of bacterial plant pathogens. crown gall, a plant disease caused by agrobacterium tumefaciens, may represent a useful model to study this effect. indeed, our previous observations on the natural occurrence and origin of nonpathogenic agrobacteria suggest that the host plant might induce loss of pathogenicity in populations of a. tumefaciens. here we report that five different a. tumefaciens strains initially isolated from appl ...19957601840
nucleotide-dependent angular change in kinesin motor domain bound to tubulin.kinesin is a 'motor' molecule, consisting of two head domains, an alpha-helical coiled coil rod, and a tail part that binds to its cargo. when expressed in a bacterial system, the head domain is functional, and can bind to microtubules with the stoichiometry of one head per tubulin dimer. kinesin moves along microtubules by means of a cyclic process of nucleotide binding, hydrolysis and product release. we have used negative-stain electron microscopy and image analysis to study the structures of ...19957617042
the control of polyphenol oxidase activity in fruits and vegetables. a study of the interactions between the chemical compounds used and heat treatment.objective of this research was to find alternative methods for the control of polyphenol oxidase (ppo) activity in fruits and vegetables with the purpose of reducing or eliminating the use of so2 for this purpose. interactions between the use of ascorbic acid, citric acid, edta, sodium metabisulphite and heat treatment (70 degrees c for 2 min) in the control of ppo activity were studied in avocado (var. fortuna), banana (var. nanica), apple (var. ana, fuji, gala & golden), pear (var. d'agua), pe ...19957659702
identification of allergens in fruits and vegetables: ige cross-reactivities with the important birch pollen allergens bet v 1 and bet v 2 (birch profilin).in this study serum samples collected from 20 patients with birch pollen allergy were investigated. all patients had experienced allergic symptoms after contact with or ingestion of particular fresh fruits and vegetables known as birch pollen-related foods.19957751517
production of patulin by different strains of penicillium expansum in pear and apple cultivars stored at different temperatures and modified atmospheres.it was shown that the ability of three different strains of penicillium expansum (nrrl 2034, nrrl 6069, and cbs 481.84) to grow and to produce patulin in pears (cv. spadona) and apples (cv. starking) varied under the different temperatures tested (0, 3, 6, 17 and 25 degrees c). strain nrrl 2034 did not produce patulin at 0 or 25 degrees c while the two other strains produced the toxin at all temperatures, the maximum production occurring at 25 degrees c (for pears) and 17 degrees c (for apples). ...19957758631
human eosinophil peroxidase enhances tumor necrosis factor and hydrogen peroxide release by human monocyte-derived macrophages.inhibition of growth or eradication of experimentally induced tumors has been shown to be accompanied by infiltration of eosinophils and macrophages into the tumor mass. since macrophages are important mediators of host antitumor activity, the possibility arises that a collaboration may exist between these two cell types in the control of tumor growth. in this study, we report the effect of eosinophil peroxidase (epo), a basic protein contained in eosinophils that binds to several cell types inc ...19957774640
the indian laburnum (cassia fistula l.) fruit: an analysis of its chemical constituents.the edible fruit tissue of indian laburnum fruit (cassia fistula l.), a member of the leguminosae family, was analyzed for certain organic compounds and mineral nutrients. of the nine macro- and micronutrients studied, k was the most highly concentrated such that 100% of the us recommended dietary allowances (rda) for adults could be met by the consumption of about 100 g of the fresh fruit. na contents in pulp and seeds are relatively low. ca content at 827 mg per 100 g of dry matter is one of t ...19957784398
[idiopathic massive osteolysis of the femur. syndrome called gorham-stout syndrome].a case of massive osteolysis (disappearing bone disease, phantom bone, gorham's massive osteolysis) was observed in a young african man with two lesions of the femur. this is a very rare syndrome characterized by an acute spontaneous resorption of bone, without any sign of malignant or infectious disorder. the lesion may develop in any part of the skeleton, with extensive bone loss, but is benign. bone is replaced by haemangiomatous tissue. the diagnosis lead on the association of clinical, radi ...19957784404
allergy to latex, avocado pear, and banana: evidence for a 30 kd antigen in immunoblotting.allergens of natural latex, latex gloves, avocado pear, and banana extracts were investigated by an immunoblotting technique in sera of patients experiencing associated latex and fruit allergies. extracts were separated by sodium dodecyl-sulfate-polyacrylamide gel electrophoresis and electroblotted onto nitrocellulose. after incubation with patients' sera, ige antibodies were revealed by a goat anti-human ige alkaline-phosphatase conjugate. seventeen serum samples from patients with well-documen ...19957852672
gluco-oligosaccharide production by a strain of erwinia amylovora.when grown in sorbitol-asparagine-salts medium, cells of erwinia amylovora isolate t83 released a mixture of oligosaccharides and lipopolysaccharide in place of extracellular polysaccharide. the oligosaccharide fraction was usually readily separable from lipopolysaccharide by gel permeation chromatography, but some samples required disaggregation by preliminary treatment with edta. by 1h- and 13c-nmr spectroscopy, methylation analysis and mass spectrometry, the oligosaccharide fraction was shown ...19957476566
promotion of gastrulation by maternal growth factor in cultured rabbit blastocysts.rabbit blastocysts of day 6 post coitus were cultured in a chemically defined, protein-free medium for 24 h. although the trophoblast continued to grow, the embryonic disc degenerated. addition of basic fibroblast growth factor (fgf-2, of human recombinant or bovine origin, 10 ng/ml) to the culture medium resulted in significant developmental progress. the embryonic disc became pear-shaped showing a round anterior edge and a posterior node. the primitive streak and hensen's node indicated that g ...19958581917
meat allergy: i--specific ige to bsa and osa in atopic, beef sensitive children.the use of lamb meat products has been suggested as an alternative diet for polyallergic children, although until now this clinical practice has not been supported by in-depth biochemical/immunological studies. the aims of this research were: to evaluate cross-reactivity between lamb and beef; to evaluate the role of bsa and osa as allergens in beef allergic children; and to evaluate cross-reactivity between bsa and osa.19958586772
the use of luciferase as a reporter for response of plant cells to the fireblight pathogen erwinia amylovora.we have introduced the gene encoding luciferase from photinus pyralis into pear and tobacco cells in order to judge the reaction of plant tissue to damaging conditions such as incubation at high temperature or inoculation with a pathogen. the constitutive expression of the luciferase gene via a strong promoter slowly decreased during propagation of the transformed pear cell line. after various stress treatments the resulting luciferase activity and the atp content of the plant cells were determi ...199524185519
context effect of common objects on visual processing.in previous experiments on visual processing it was shown that correct identification of sequentially and simultaneously presented numerals declined as the presentation interval was decreased from 50.0 to 16.7 ms/numeral. to determine how performance was influenced in context, common everyday objects were used in place of the numerals. these objects were grouped into 36 different categories with 4 objects per category. an example of a category was fruit: apple, banana, pear, pineapple. for all e ...19958539008
molecular cloning of cdnas encoding the protein backbones of arabinogalactan-proteins from the filtrate of suspension-cultured cells of pyrus communis and nicotiana alata.this paper reports the isolation of cdnas encoding the protein backbone of two arabinogalactan-proteins (agps), one from pear cell suspension cultures (agppc2) and the other from suspension cultures of nicotiana alata (agpna2). the proteins encoded by these cdnas are quite different from the 'classical' agp backbones described previously for agps isolated from pear suspension cultures and extracts of n. alata styles. the cdna for agppc2 encodes a 294 amino acid protein, of which a relatively sho ...19957670506
cognitive deficits in schizophrenia. prediction of symptom change for participators in work rehabilitation.while recent studies have found that most patients with schizophrenia who participate in work rehabilitation experience symptom improvement, there appears to be a subgroup of patients whose symptoms remain stable or even worsen. the present study explores the hypothesis that cognitive impairment is one factor which reduces the clinical effectiveness of rehabilitation and accounts for a lack of improvement in some patients. to explore this question, performance on two neuropsychological tests was ...19957745389
[gorham-stout massive ostelysis. a case report].one case of massive osteolysis gorham-stout is presented. idiopathic osteolysis is a rare disease. the coincidental radiological diagnosis following minor trauma or a few months' history of mild pain involving joints is characteristic. the disease process has a sudden onset. bones undergo partial or complete resorption, which may spread across joints to contiguous bones. the osteolytic process eventually ceases spontaneously, but generally not before severe deformation and destruction of bone ha ...19957709224
massive osteolysis of the pelvis, femur and sacral bone with a gorham-stout syndrome.we report a case of a 49-year-old white woman with gorham-stout syndrome. this very rare massive osteolysis occurred in the left pelvis and included the femur and the sacral bone. histology study revealed typical ectatic vessels covered with endothelium. the therapy of choice in this region is radiotherapy with a dose of 40 gy. however, in our case this therapy only led to short-term pain relief and cessation of the osteolysis. stabilization with corticospongious bone did not seem to be effectiv ...19957662475
efficientagrobacterium-mediated transformation and recovery of transgenic plants from pear (pyrus communis l.).an efficient and reproducible method was established for genetic transformation of one pear variety (conferénce) usingagrobacterium tumefaciens-mediated gene transfer. wounded leaves of in vitro micropropagated plants were cocultivated with the disarmed strain eha101 harbouring the binary vector pfaj3000 carrying the chimaericnptii andgus genes. the protocol included a 3-6 month dark period on a regeneration medium solidified with gelrite, which contained 100 mg/l kanamycin. up to 42% of inocula ...199624177562
massive osteolysis (gorham-stout syndrome) in the maxillofacial region: an unusual manifestation.an unusual case of massive osteolysis affecting the left side of the mandible, the maxilla, the zygoma, and the orbit is reported. the disease extended over a period of 25 years. the special feature of this case is that there were, before the osteolysis appeared, enlargement and sclerosis of the maxillofacial bones reaching up to the middle cranial fossa. histopathologic examination of the affected mandible revealed that the bone was replaced by fibrous tissue.19968961021
efficacy of nonpheromone communication disruptants of codling moth (cydia pomonella): effect of pheromone isomers and of distance between calling females and dispensers.field tests comparing the ability of codlemone, (e,e)-8,10-dodecadien-1-ol, and isomers of codlemone to disrupt pheromonal communication of codling moth were carried out. in a pear orchard, four nonisomerizing, gray septa dispensers were placed in the upper canopy of each tree containing a trap baited with 10 virgin female codling moths. the dispensers were at trap height and 70 cm from the edge of each trap. trap catches of released male codling moths in three test areas were compared simultane ...199624227482
hypolipidaemic foods in china.with the changes in dietary pattern in china, in recent years, hyperlipidaemia has become an important problem in the pathogenesis of chronic degenerative diseases, especially the cardio-cerebro-vascular diseases. from studies on laboratory animals and people with hyperlipidaemia, a number of hypolipidaemic foods and beverages have been identified, of relevance to traditional chinese food culture. their absence from the diet may, in part account for the increasing prevalence of hyperlipidaemia i ...199624394619
interleukin-6: a potential mediator of the massive osteolysis in patients with gorham-stout disease.gorham-stout disease (gsd) or massive osteolysis, is an extremely rare osteolytic condition that involves extensive locally aggressive resorption of bone. the etiology and pathophysiology are unknown, and the role of the osteoclast in gsd is unclear. we studied a patient with gsd who had massive resorption of his mandible, which extended to his maxilla, zygoma, right parietal region, and cranium. to investigate the cause of the extensive resorption, we tested the effects of the patient's serum, ...19968626854
self-incompatibility (s) alleles of the rosaceae encode members of a distinct class of the t2/s ribonuclease superfamily.stylar ribonucleases (rnases) are associated with gametophytic self-incompatibility in two plant families, the solanaceae and the rosaceae. the self-incompatibility-associated rnases (s-rnases) of both the solanaceae and the rosaceae were recently reported to belong to the t2 rnase gene family, based on the presence of two well-conserved sequence motifs. here, the cloning and characterization of s-rnase genes from two species of rosaceae, apple (malus x domestica) and japanese pear (pyrus seroti ...19968676858
phytoplasma-specific pcr primers based on sequences of the 16s-23s rrna spacer region.in order to develop a diagnostic tool to identify phytoplasmas and classify them according to their phylogenetic group, we took advantage of the sequence diversity of the 16s-23s intergenic spacer regions (srs) of phytoplasmas. ten pcr primers were developed from the sr sequences and were shown to amplify in a group-specific fashion. for some groups of phytoplasmas, such as elm yellows, ash yellows, and pear decline, the sr primer was paired with a specific primer from within the 16s rrna gene. ...19968702291
genetic transfer of amylovoran and stewartan synthesis between erwinia amylovora and erwinia stewartii.dna fragments with ams genes of erwinia amylovora and cps genes of erwinia stewartii were transferred to exopolysaccharide (eps)-deficient mutants of the other species. the resulting epss were characterized by sensitivity to eps-dependent bacteriophages, staining with amylovoran-specific fluorescein-isothiocyanate-labelled lectin and chemical techniques, such as determination of the sugar composition and methylation analysis in order to distinguish between amylovoran and stewartan. degradation b ...19968704950
morphological indications of hair cell neodifferentiation in the organ of corti of amikacin treated rat pups.postnatal rats were treated with amikacin during the period of cochlear supra-normal sensitivity to ototoxic antibiotics, i.e. from day 9 to day 16 when the organ of corti is achieving its maturation. scanning and transmission electron microscopies were used to evaluate morphological changes in the organs of corti at different post-treatment periods, up until postnatal day 90. a massive loss in outer (ohcs) and inner (ihcs) hair cells was observed from as early as day 17. a prominent feature in ...19968762976
determination of avermectin b1 in biological samples by immunoaffinity column cleanup and liquid chromatography with uv detection.a simple and specific procedure for determination of avermectin b1 in biological samples is described. a specific anti-avermectin b1 immunosorbent was developed. avermectin b1 was extracted from cattle plasma, cattle meat, and pear samples. the extracts were cleaned up on a solid-phase extraction column (c18) and an immunoaffinity column. avermectin b1 was determined by reversed-phase liquid chromatography with uv detection at 245 nm. recoveries of avermectin b1 from samples spiked at levels of ...19968823915
left intraventricular balloon pump optimization during intractable cardiac arrest.this work aims to determine optimal balloon shape and volume during left intraventricular balloon pumping (iabp) in the fibrillating dog heart. a balloon volume equal to the left ventricular end-diastolic volume (lvedv) maintained a higher systolic aortic pressure and flow (106.4 +/- 2.7 mmhg and 84.7 +/- 2.35 ml/kg/min, x +/- sem, respectively) than a 25% smaller (97.8 +/- 3.3 mmhg, p = 0.002 and 63.7 +/- 4.1 ml/kg/min, p = 0.002, respectively) or a 25% larger balloon (87.4 +/- 2.3 mmhg, p = 0. ...19968841857
identification and partial amino acid sequences of seven s-rnases associated with self-incompatibility of japanese pear, pyrus pyrifolia nakai.s-allele-specific proteins (s-proteins) were separated and identified by two-dimensional (2d) gel electrophoresis from the style extract of 14 cultivars of japanese pear, pyrus pyrifolia nakai, which exhibits gametophytic self-incompatibility. these s-proteins were 30-32 kda basic proteins with putative pis of 9.6-10.1 and were distinct from the other proteins, which were common for all cultivars examined. each s-protein was assigned to a given s-genotype based on electrophoretic mobility and th ...19968889818
aflatoxin and cyclopiazonic acid production by a sclerotium-producing aspergillus tamarii strain.the production of aflatoxins b1 and b2 by aspergillus tamarii (subgenus circumdati section flavi) is reported for the first time. the fungus was isolated from soil collected from a tea (camellia sinensis) field in miyazaki prefecture, japan. three single-spore cultures, nrrl 25517, nrrl 25518, and nrrl 25519, were derived from subcultures of the original isolate 19 (mz2). each of these single-spore cultures of a. tamarii produced aflatoxins b1 and b2 and cyclopiazonic acid, as well as black, pea ...19968899995
ige binding to a new cross-reactive structure: a 35 kda protein in birch pollen, exotic fruit and other plant foods.food allergies in birch pollen allergic patients have been shown to be due to cross-reactivities of specific ige antibodies which are directed against birch pollen allergens with related proteins in fruit, nuts and vegetables. we identified a new cross-reactive structure of 35 kda in birch pollen and some plant food extracts by enzyme allergosorbent test (east) and immunoblot inhibition studies. the 35 kda birch pollen protein is a minor allergen to which approximately 10-15% of birch pollen all ...19969000332
enumeration of bacterial and yeast colonists of apple fruits and identification of epiphytic yeasts on pear fruits in the pacific northwest united states.a procedure for the isolation of diverse culturable microflora for the estimation of the population size of yeasts and bacteria on the surface of pome fruits is described. maximum numbers of morphologically distinct colonies of both yeasts and bacteria were recovered from apple fruit surfaces when fruits were shaken for 5 min in sterile phosphate buffer plus tween, sonicated for 5 min, and aliquots of the buffer plated onto diluted yeast malt agar and diluted nutrient broth agar, respectively. t ...19969022303
location of cysteine and cystine residues in s-ribonucleases associated with gametophytic self-incompatibility.s-ribonucleases (s-rnases) that cosegregate with s-alleles in the styles of solanaceous and rosaceous plants are associated with gametophytic self-incompatibility (gsi). the amino acid sequences of many s-rnases have been derived from cdna sequences, but the state of half-cystines has not been clarified. we report the locations of the two free cysteine residues and four disulfide bridges of tobacco s6-rnase and of the four disulfide bridge of japanese pear s4-rnase. the protein was first s-pyrid ...19969022690
electro-optical imaging of f-actin and endoplasmic reticulum in living and fixed plant cells.confocal and video micrographs of living and fixed alfalfa roots, onion epithelial and pear pollen cells illustrate the architecture of the cytoskeleton and endoplasmic reticulum in plant cells. fixation of plant tissues to preserve cytoplasmic structure poses special problems. when possible, emphasis should be placed on the imaging of structures in stained living cells over time. the early events that occur when nod factors or bacteria elicit nodule formation in alfalfa roots will illustrate se ...19969601538
the regulatory subunits of fission yeast protein phosphatase 2a (pp2a) affect cell morphogenesis, cell wall synthesis and cytokinesis.protein phosphatase 2a (pp2a) holoenzymes have a trimeric structure, consisting of a catalytic subunit c and two regulatory subunits a (pr65) and b (pr55). in fission yeast the c subunits, being 80% identical to their mammalian counterparts, are essential for viability and negatively regulate the entry into mitosis. genetic analyses in budding yeast and drosophila show that the regulatory subunits are implicated in chromosome segregation, cell morphogenesis and/or cytokinesis.19969078365
observations on the micromorphology of the tropical rat mite ornithonyssus bacoti (hirst) as revealed by scanning electron microscopy.ornithonyssus bacoti (hirst) is an important parasite of rodents. the characteristic shape of the dorsal and sternal shields and gnathosoma used as taxonomic characteristics was confirmed by scanning electron microscopy. the anal plate is distinctly pear-shaped with a pair of adanal setae lateral to the anal valves, a single postanal seta and a distinct dentate cribrum formed by peg-like projections. in the adult mites a peritreme extends anteriorly to the 1st coxa from the spiracle situated lat ...19969120855
a purified extract from prickly pear cactus (opuntia fuliginosa) controls experimentally induced diabetes in rats.the hypoglycemic activity of a purified extract from prickly pear cactus (opuntia fuliginosa) was evaluated on stz-induced diabetic rats. blood glucose and glycated hemoglobin levels were reduced to normal values by a combined treatment of insulin and opuntia extract. when insulin was withdrawn from the combined treatment, the prickly pear extract alone maintained normoglycemic state in the diabetic rats. the blood glucose response to administered glucose also showed that the rats receiving the ...19969121164
in vitro prenylation of the small gtpase rac13 of cotton.previous work (d.p. delmer, j. pear, a. andrawis, d. stalker [1995] mol gen genet 248: 43-51) has identified a gene in cotton (gossypium hirsutum), rac13, that encodes a small, signal-transducing gtpase and shows high expression in the fiber at the time of transition from primary to secondary wall synthesis. since rac13 may be important in signal transduction pathway(s), regulating the onset of fiber secondary wall synthesis, we continue to characterize rac13 by determining its ability to underg ...199612226460
characterization of erwinia amylovora strains using random amplified polymorphic dna fragments (rapds).the genetic diversity among 16 strains of erwinia amylovora, chosen to represent different host plant origins and geographical regions, was investigated by rapd analysis. one strain of erwinia herbicola and one of agrobacterium vitis were used as outgroups. ninety-eight different rapd fragments were produced by polymerase chain reaction amplification with six different 10-mer primers. rapd banding profiles were found that enabled the erw. amylovora strains to be distinguished from one another. c ...199712455904
determination of ethylenethiourea in food commodities by a two-step derivatization method and gas chromatography with electron-capture and nitrogen-phosphorus detection.ethylenethiourea (etu) is a decomposition product from ethylene-bis-dithiocarbamates (ebdcs), the most widely used class of fungicides in the world. etu has been classified as a possible human carcinogen. the maximum permitted residue level (mrl) in the european union was set at 0.05 ppm. gas chromatographic determination of etu can be achieved only after derivatization. etu is extracted from food samples and cleaned up by a combination of two-step derivatization and liquid-liquid partitioning. ...19979129299
circular dna plasmid in the phytopathogenic fungus alternaria alternata: its temperature-dependent curing and association with pathogenicity.we found the presence of plasmid dna in strain t88-56 of the japanese pear pathotype of alternaria alternata, which causes black spot of certain cultivars of japanese pear by producing host-specific ak-toxin. the plasmid, designated paat56, was identified to be an approximately 5.4-kilobase (kb) circular molecule by electron microscopic observation and restriction endonuclease mapping. southern blot analysis showed that paat56 dna had no homology with either nuclear or mitochondrial dna. culture ...19979136005
three-dimensional structure of the porcine gastric h,k-atpase from negatively stained crystals.a low-resolution three-dimensional model of membrane-bound h,k-atpase from pig gastric mucosa has been reconstructed by electron microscopy and image processing of two-dimensional crystals in negative stain. the crystal formation is induced by magnesium and vanadate, which stabilize the e2 conformation of the enzyme. the unit cell, with a size of a = b = 123 a, gamma = 90 degrees, has tetragonal p4 symmetry. there are four separate alpha beta protomers within each unit cell. the high-contrast re ...19979169227
immunoelectron-microscopic investigation of the subcellular localization of pinopsin in the pineal organ of the chicken.pinopsin is a photoreceptive molecule cloned from the chicken pineal organ. an antibody highly specific for pinopsin was applied in light- and electron-microscopic immunocytochemical studies of the pineal organ of 1 to 2-month-old chickens. intense immunoreactivity was found in the follicular lumen at the light-microscopic level. in addition, small immunoreactive spherical or fibrous structures were diffusely distributed at the parafollicular aspect of the pineal organ. to identify immunoreactiv ...19979211826
does the organ of corti attempt to differentiate new hair cells after antibiotic intoxication in rat pups?in the adult mammalian cochlea, post-injury hair cell losses are considered to be irreversible. recent studies in cochlear explants of embryonic rodents show that the organ of corti can replace lost hair cells after injury. we have investigated this topic in vivo during the period of cochlear development. rat pups were treated with a daily subcutaneous injection of 500 mg/kg amikacin for eight consecutive days between postnatal day 9 (pnd 9) and pnd 16. during this period the organ of corti is n ...19979263027
changes in h(+)-pumps and a tonoplast intrinsic protein of vacuolar membranes during the development of pear fruit.vacuolar h(+)-atpase (v-atpase) was purified from pear fruit and antibodies were raised against the subunits of 55 and 33 kda. antibodies against mung bean h(+)-pyrophosphatase (v-ppase) and radish vm23, which is a tonoplast intrinsic protein (tip) and a water channel, cross-reacted with the vacuolar membrane proteins of pear fruit. to clarify the roles of these proteins in development of pear fruit, we determined their levels relative to the total amount of protein by immunoblot analysis. the l ...19979360322
structural analysis of the plasmid paat56 of the filamentous fungus alternaria alternata.the circular dna plasmid, designated paat56, has been isolated from strain t88-56 of the japanese pear pathotype of alternaria alternata. we determined the complete nucleotide sequence (5354 bp) of paat56 and mapped its possible open reading frames (orfs). three long orfs, orf1 (1290 bp), orf2 (1653 bp) and orf3 (690 bp), and four smaller orfs, orf4 to orf7 (> or = 300 bp), were predicted from the sequence. the potential peptides derived from the orfs other than orf2 show no homology to other kn ...19979426006
dspa, an essential pathogenicity factor of erwinia amylovora showing homology with avre of pseudomonas syringae, is secreted via the hrp secretion pathway in a dspb-dependent way.in erwinia amylovora, the dsp region, required for pathogenicity on the host plant but not for hypersensitive elicitation on tobacco, is separated from the hrp region by 4 kb. the genetic analysis reported in this paper showed that this 4kb region is not required for pathogenicity on pear seedlings. the environmental conditions allowing expression of a dsp::lacz fusion were examined: expression was barely detected in rich medium at 30 degrees c, and the highest expression was observed in m9 gala ...19979426142
allergy to rosaceae fruits without related pollinosis.rosaceae fruit allergy is frequently associated with birch pollinosis in central and northern europe and with grass pollen allergy in central spain. the main cross-reactive structures involved for birch pollinosis are bet v 1 and profilin, and for grass pollinosis they are profilin and carbohydrate determinants. rosaceae fruit allergy can occasionally be observed in patients without pollinosis.19979438478
an unidentified species of theileria infective for cattle discovered in china.infection experiments, morphological observations and transmission experiments were conducted with an unidentified theileria sp. isolated from a naturally infected ox. the results showed that the protozoa could multiply extensively in a splenectomized ox and the parasitaemia could reach 52.69%. the theileria sp. was polymorphic: being pear-shaped, circular, elliptical, rod-like, comma-shaped, three-leafed- or cross-shaped and having many other irregular-shapes which were seldom detected. in eryt ...19979512745
a simple and rapid method for isolation of high quality genomic dna from fruit trees and conifers using pvp.because dna degradation is mediated by secondary plant products such as phenolic terpenoids, the isolation of high quality dna from plants containing a high content of polyphenolics has been a difficult problem. we demonstrate an easy extraction process by modifying several existing ones. using this process we have found it possible to isolate dnas from four fruit trees, grape (vitis spp.), apple (malus spp.), pear (pyrus spp.) and persimmon (diospyros spp.) and four species of conifer, pinus de ...19979023124
the types of neurons of the somatic oculomotor nucleus in the european bison. nissl and golgi studies.the neuronal structure of the somatic oculomotor nucleus (son) was studied on the basis of nissl and golgi preparations, obtained from mesencephalons of 4 european bisons. we distinguished four types of neurons in the investigated nucleus: 1. the large multipolar nerve cells with 5-8 thick dendritic trunks and a thin axon which emerges directly from the soma. these are the most numerous neurons in the son. 2. the small multipolar neurons. these cells have 4-6 thick dendritic trunks. an axon aris ...19979635352
crab apple blossoms as a model for research on biological control of fire blight.abstract nonseasonal availability of pomaceous flowers could improve laboratory detection and prefield testing of biocontrol agents for fire blight of pear and apple. crab apple was selected as a model because of its high flower productivity on 1-year-old wood, high susceptibility to fire blight, and availability from nurseries. cultivars manchurian and snowdrift were manipulated to bloom once by transferring dormant nursery trees from a cold room to a greenhouse and a second time by defoliating ...199718945005
aal-toxin-deficient mutants of alternaria alternata tomato pathotype by restriction enzyme-mediated integration.abstract host-specific toxins are produced by three pathotypes of alternaria alternata: am-toxin, which affects apple; ak-toxin, which affects japanese pear; and aal-toxin, which affects tomato. each toxin has a role in pathogenesis. to facilitate molecular genetic analysis of toxin production, isolation of toxin-deficient mutants utilizing ectopic integration of plasmid dna has been attempted. however, the transformation frequency was low, and integration events in most transformants were compl ...199718945069
molecular variability of the genomes of capilloviruses from apple, japanese pear, european pear, and citrus trees.abstract the 3'-terminal regions of the genomes of apple stem grooving virus (asgv), isolated from apple, japanese pear, and european pear plants, and citrus tatter leaf virus (ctlv), isolated from citrus plants, were amplified by reverse transcription-polymerase chain reaction. the dna products were cloned and sequenced. the results indicated that the asgv isolates from apple, japanese pear, and european pear comprise at least two to four "sequence variants" that differ considerably from each o ...199718945117
effects of water stress cycles on turgor maintenance processes in pear leaves (pyrus communis).effects of water deficits on leaf turgor maintenance processes were analyzed for pear trees (pyrus communis l. cv. "barlett") grown in 120-liter containers. four irrigation treatments were applied: a well-watered control treatment, a spring water stress cycle (sp), a summer water stress cycle (su), and a spring plus summer water stress cycle (sp + su). for the sp treatment, water application was progressively reduced from 100 to 20% of the control dose over a period of 27 days in spring. for the ...199714759856
the prevalence of latex allergy in children seen in a university hospital allergy clinic.natural rubber latex allergy is responsible for a wide spectrum of clinical symptoms, ranging from rhinoconjunctivitis to severe anaphylaxis, in both adults and children. an association between allergy to latex and allergy to various fruits has been reported. this study investigated the prevalence and clinical significance of latex sensitization in children seen in a university hospital allergy clinic. a total of 453 consecutive children were screened in a 7-month period. a detailed clinical his ...19979062637
octavolateral neurons projecting to the middle and posterior rhombencephalic reticular nuclei of larval lamprey: a retrograde horseradish peroxidase labeling study.the octavolateral area of lampreys, which receives primary fibers from the octaval and lateral line nerves, is involved in the premotor organization of body movements through secondary projections to the reticular formation. here, the typology of neurons of the three octavolateral nuclei (ventral, medial, and dorsal) that putatively project to the middle and posterior rhombencephalic reticular nuclei were studied by retrograde transport of horseradish peroxidase (hrp) applied to these reticular ...19979254035
disappearing bone disease (gorham-stout disease): report of a case with a follow-up of 48 years.a patient with osteolysis of the right hand after a metatarsal fracture at the age of 12 years is reported. the osteolysis progressed until the age of 21 years and was stable until the age of 59 years, when the patient died from a metastatic colon cancer. the article discusses the clinical, radiographic, and histologic features, and prognosis of idiopathic osteolysis (gorham-stout disease). gorham-stout disease is characterized by a non-familial, histological benign vascular proliferation produc ...19979348269
[radiological case of the month. gorham disease of costovertebral localization]. 19979345574
outcome of clozapine therapy for elderly patients with refractory primary psychosis.the objective was to analyze outcome of clozapine therapy in elderly patients with treatment refractory primary psychosis.19979193964
effects of chilling on the expression of ethylene biosynthetic genes in passe-crassane pear (pyrus communis l.) fruits.passe-crassane pears require a 3-month chilling treatment at 0 degrees c to be able to produce ethylene and ripen autonomously after subsequent rewarming. the chilling treatment strongly stimulated acc oxidase activity, and to a lesser extent acc synthase activity. at the same time, the levels of mrnas hybridizing to acc synthase and acc oxidase probes increased dramatically. fruit stored at 18 degrees c immediately after harvest did not exhibit any of these changes, while fruit that had been pr ...19979106508
[kasabach-merritt syndrome of the leg associated with osteolysis or gorham sign].kasabach-merritt syndrome and gorham's sign are two uncommon and severe, sometimes life-threatening, complications in infants with vascular lesions. their association has been described in rare cases.19979732759
gorham massive osteolysis.gorham syndrome (massive osteolysis) is a very rare tumour-like lesion characterized by progressive osteolysis. the diagnosis must be confirmed by the microscopic finding of intramedullary angioma-like vascular structures. we report a case of a 15-year-old boy with a pathological fracture in his left humerus. imaging modalities such as magnetic resonance imaging, computed tomography, angiography and bone scintigraphy failed to disclose to tumorous lesion that filled a cavity in the left humerus. ...19979352051
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