Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
a novel pathway for the biodegradation of gamma-hexachlorocyclohexane by a xanthomonas sp. strain ich12.to isolate gamma-hexachlorocyclohexane (hch)-degrading bacteria from contaminated soil and characterize the metabolites formed and the genes involved in the degradation pathway.200717578411
aerobic degradation of lindane (gamma-hexachlorocyclohexane) in bacteria and its biochemical and molecular basis.gamma-hexachlorocyclohexane (gamma-hch, also called gamma-bhc and lindane) is a halogenated organic insecticide that causes serious environmental problems. the aerobic degradation pathway of gamma-hch was extensively revealed in bacterial strain sphingobium japonicum (formerly sphingomonas paucimobilis) ut26. gamma-hch is transformed to 2,5-dichlorohydroquinone through sequential reactions catalyzed by lina, linb, and linc, and then 2,5-dichlorohydroquinone is further metabolized by lind, line, ...200717634937
degradation of 3-o-methylgallate in sphingomonas paucimobilis syk-6 by pathways involving protocatechuate 4,5-dioxygenase.sphingomonas paucimobilis syk-6 converts vanillate and syringate to protocatechuate and 3-o-methylgallate (3mga), respectively. 3mga is metabolized via multiple pathways involving 3mga 3,4-dioxygenase, protocatechuate 4,5-dioxygenase (ligab), and gallate dioxygenase whereas protocatechuate is degraded via the protocatechuate 4,5-cleavage pathway. here the secondary role of ligab in syringate metabolism is investigated. the reaction product of 3mga catalyzed by his-tagged ligab was identified as ...200717645527
bacterial contamination of water in dental unit reservoirs.the aim of this study was bacteriological assessment of water in dental unit reservoirs--concentration and composition of the aerobe and facultative anaerobe bacterial microflora. reservoir water samples were taken from 25 units. bacterial flora were determined with the plate culture method. bacteria were identified with biochemical microtests: api 20e, api 20ne (biomérieux, france) and gp2 microplatetm (biolog, usa). the concentration of total bacteria isolated from one site was 201,039 cfu/ml, ...200717655191
4-sulfomuconolactone hydrolases from hydrogenophaga intermedia s1 and agrobacterium radiobacter s2.the 4-carboxymethylen-4-sulfo-but-2-en-olide (4-sulfomuconolactone) hydrolases from hydrogenophaga intermedia strain s1 and agrobacterium radiobacter strain s2 are part of a modified protocatechuate pathway responsible for the degradation of 4-sulfocatechol. in both strains, the hydrolase-encoding genes occur downstream of those encoding the enzymes that catalyze the lactonization of 3-sulfomuconate. the deduced amino acid sequences of the 4-sulfomuconolactone hydrolases demonstrated the highest ...200717660282
characterization of ligv essential for catabolism of vanillin by sphingomonas paucimobilis syk-6.the vanillin dehydrogenase gene (ligv), which conferred the ability to transform vanillin into vanillate on escherichia coli, was isolated from sphingomonas paucimobilis syk-6. the ligv gene consists of a 1,440-bp open reading frame encoding a polypeptide with a molecular mass of 50,301 da. the deduced amino acid sequence of ligv showed about 50% identity with the known vanillin dehydrogenases of pseudomonas vanillin degraders. the gene product of ligv (ligv) produced in e. coli preferred nad+ t ...200717928721
nosocomial outbreak of sphingomonas paucimobilis bacteremia in a hemato/oncology unit.nosocomial sphingomonas paucimobilis infections can arise from contaminated water and the contaminated hands of hospital staff. within a 1-month period, we isolated six s. paucimobilis strains, including four from blood cultures of four patients and two from hospital environment specimens including tap water and a bathtub in a hemato/oncology unit. we described here these strains' molecular epidemiological analyses by pulsed-field gel electrophoresis (pfge) and antibiotic susceptibilities by e-t ...200718032843
optimization of nutrients for gellan gum production by sphingomonas paucimobilis atcc-31461 in molasses based medium using response surface methodology.a molasses based medium for the production of gellan by sphingomonas paucimobilis atcc-31461 was developed. placket-burman design criterion was applied to study the effect of various nutrient supplements on gellan production using molasses. among the 20 variables tested, molasses, tryptone, casaminoacid, disodium hydrogen orthophosphate and manganese chloride showed significant effect on gellan production. a central composite design was applied to determine the optimum concentrations of the sign ...200716707262
enhanced degradation of a mixture of polycyclic aromatic hydrocarbons by a defined microbial consortium in a two-phase partitioning bioreactor.biological treatment methods are effective at destroying polycyclic aromatic hydrocarbons (pahs), and some of the highest rates of pah degradation have been achieved using two-phase-partitioning bioreactors (tppbs). tppbs consist of a cell-containing aqueous phase and a biocompatible and immiscible organic phase that partitions toxic and/or recalcitrant substrates to the cells based on their metabolic demand and on maintaining the thermodynamic equilibrium of the system. in this study, the degra ...200716758271
evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting gram-negative rods.to evaluate the activity of pyrrolidonyl arylamidase (pyr) for the differentiation and identification of nonfermenting gram negative rods (nfgnr), 293 isolates were tested. a 24 h culture of each test organism was prepared. from this a 108-109 cfu/ml suspension was added to 0.25 ml of sterile physiologic solution. a pyr disk was then added and the test was incubated for 30 minutes at 35-37 degrees c, at environmental atmosphere. reading was done by adding 1 drop of cinnamaldehyde reagent. strain ...200716822636
[contamination with sphingomonas paucimobilis: about seven cases isolated in conservation and transport mediums of corneal grafts].from september to december 2004, contaminations were found in fifteen conservation and transport mediums of corneal grafts at the tissue bank of brest, including seven by sphingomonas paucimobilis. the pulsed-field gel electrophoresis made it possible to establish the genotypic profiles of each strain and to compare them. similarities were found between certain strains of the contaminated mediums and those of the thermostated double boiler of the tissue bank. the link between the contamination a ...200716631318
periplasmically located α-santonin binding factor in sphingomonas paucimobilis strain s atcc 43388.a marked reduction in uptake of α-santonin, accompanied by loss of ability of cells to transform the substrate, is observed on shocking sphingomonas paucimobilis strain s atcc 43388 cells by freeze - thaw method. the shock fluid shows a 26% quench in fluorescence at 350nm on incubation with the substrate. addition of shock fluid to the freeze thawed cells restores both uptake as well as transformation of α-santonin to near normal.200723100690
arbuscular mycorrhiza and petroleum-degrading microorganisms enhance phytoremediation of petroleum-contaminated soil.while plants can phytoremediate soils that are contaminated with petroleum hydrocarbons, adding microbes to remediate contaminated sites with petroleum-degrading microorganisms and arbuscular mycorrhizal fungi (amf) is not well understood. the phytoremediation of arabian medium crude oil (aco) was done with a lolium multiflorum system inoculated with an amf (glomus intraradices) and a mixture of petroleum-degrading microorganisms--the bacterium, sphingomonas paucimobilis (sp) and the filamentous ...200819260211
[a rare cause of nosocomial bacteremia: sphingomonas paucimobilis].sphingomonas paucimobilis, is a yellow-pigmented, aerobic, non-fermentative, non-spore-forming, gram-negative bacillus. infections by s. paucimobilis which is widely found in nature and hospital environments are rarely serious or life threatening. in this report we present a case of hospital acquired bloodstream infection due to s. paucimobilis. the patient had a history of hydrocephalus diagnosed at sixth months of his birth and had experienced two ventriculoperitoneal shunt surgery. he was hos ...200819149092
the negative interaction between the degradation of phenanthrene and tricyclazole in medium, soil and soil/compost mixture.to assess the co-catabolism of phenanthrene and tricyclazole in different samples, the interaction during the degradation of phenanthrene and tricyclazole were investigated in medium, soil and soil/spent mushroom compost (smc) mixture. generally, tricyclazole showed a negative influence on the activity of phenanthrene dioxygenase and it inhibited the degradation of phenanthrene prominently, both in cultures of phenanthrene catabolic isolates (sphingomonas paucimobilis zx4 and the mixed flora m1) ...200818189158
extracellular polysaccharides produced by cooling water tower biofilm bacteria and their possible degradation.the extracellular polymers (eps) of biofilm bacteria that can cause heat and mass transfer problems in cooling water towers in the petrochemical industry were investigated. in addition, these microorganisms were screened for their ability to grow and degrade their own eps and the eps of other species. twelve bacteria producing the most eps were isolated from cooling water towers and characterized biochemically by classic and commercial systems. these were species of pseudomonas, burkholderia, ae ...200818256966
a case of postoperative sphingomonas paucimobilis endophthalmitis after cataract extraction.to report a case of an acute onset of delayed postoperative endophthalmitis that was caused by sphingomonas paucimobilis.200818323709
quantitative structure-activity relationships for kinetic parameters of polycyclic aromatic hydrocarbon biotransformation.quantitative structure-activity relationships (qsars) were developed for three monod-type parameters--qmax, ks, and qmax/ks--that express the kinetics of polycyclic aromatic hydrocarbon (pah) biotransformation by sphingomonas paucimobilis strain epa505. the training sets contained high-quality experimental values of the kinetic parameters for 20 unsubstituted and methylated pahs as well as values of 41 meaningful molecular descriptors. a genetic function approximation algorithm was used to devel ...200818366261
optimization of gellan gum production by sphingomonas paucimobilis atcc 31461 with nonionic surfactants using central composite design.the effect of nonionic surfactants on gellan production by sphingomonas paucimobilis was studied by the addition of 0.5, 0.75, 1.0, 1.25 and 1.5 g/l surfactants to shake flask culture. the nonionic surfactants tween 80, tween 40 and triton x-100 improved gellan production by s. paucimobilis, and the maximum yield (10.44 g/l) was obtained with triton x-100 at 0.75 g/l compared with that of the control fermentation (8.63 g/l) without surfactant. the do profiles associated with gellan production in ...200818397769
isolation and characterization of marine bacterial strain degrading fucoidan from korean undaria pinnatifida sporophylls.in spite of an increasing interest in fucoidans as biologically active compounds, no convenient commercial sources with fucoidanase activity are yet available. a marine bacterial strain that showed confluent growth on a minimal medium containing fucoidan, prepared from korean undaria pinnatifida sporophylls, as the sole carbon source was isolated and identified based on a 16s rdna sequence analysis as a strain of sphingomonas paucimobilis, and named sphingomonas paucimobilis pf-1. the strain dep ...200818467852
assessment of inhibitory effects of fluoride-coated tubes on biofilm formation by using the in vitro dental unit waterline biofilm model.this study aimed to establish an in vitro model to simulate biofilms formed in dental unit waterlines (duwls) and to investigate the ability of polyvinylidene fluoride (pvdf)-coated tubes to inhibit biofilm formation using this model. the water and biofilm samples were obtained from duwls which had been clinically used for 2.5 years, and the predominant bacteria were identified. a conventional polyurethane tube was incubated for 24 to 96 h in the mixed flora of isolated bacteria, and the optimal ...200818676694
sphingomonas paucimobilis peritonitis: a case report and literature review. 200818708553
sphingomonas paucimobilis associated with localised calf myositis. 200818796597
clinical features and treatment outcomes of infections caused by sphingomonas paucimobilis. 200818808348
acclimated biomass that degrades sulfonated naphthalene formaldehyde condensate.a number of aerobic species were isolated from textile industry activated sludge wastewater. the bacterial consortium was acclimated during seven days before testing its capacity of sulfonated naphthalene-formaldehyde condensate (snfc) recalcitrant compound degradation. snfc's degradation was evaluated by using different techniques including: vapour pressure osmometry, spectroscopy uv-visible and chemical oxygen demand (cod). the degradation of snfc by acclimated bacterial consortium was determi ...200818819646
interaction of higher plant (jute), electrofused bacteria and mycorrhiza on anthracene biodegradation.the interaction of bacteria, mycorrhiza and jute (corchotus capsulari, a higher plant) to reduce anthracene in different concentrations of spiked soils was investigated. dominant indigenous bacterium (pseudomonas sp.) isolated in the rhizosphere of jute was electrofused with anthracene degraders (sphingomonas paucimobilis and pseudomonas aeruginosa) which were able to produce different types of biosurfactants. the highest population (56 x 10(5)cfu/g) was found in the planted soil with the inocul ...200817662599
effects of the inoculant strain sphingomonas paucimobilis 20006fa on soil bacterial community and biodegradation in phenanthrene-contaminated soil.the effects of the inoculant strain sphingomonas paucimobilis 20006fa (isolated from a phenanthrene-contaminated soil) on the dynamics and structure of microbial communities and phenanthrene elimination rate were studied in soil microcosms artificially contaminated with phenanthrene. the inoculant managed to be established from the first inoculation as it was evidenced by denaturing gradient gel electrophoresis analysis, increasing the number of cultivable heterotrophic and pah-degrading cells a ...200817694405
second step of hydrolytic dehalogenation in haloalkane dehalogenase investigated by qm/mm methods.mechanistic studies on the hydrolytic dehalogenation catalyzed by haloalkane dehalogenases are of importance for environmental and industrial applications. here, car-parrinello (cp) and oniom hybrid quantum-mechanical/molecular mechanics (qm/mm) are used investigate the second reaction step of the catalytic cycle, which comprises a general base-catalyzed hydrolysis of an ester intermediate (ei) to alcohol and free enzyme. we focus on the enzyme linb from sphingomonas paucimobilis ut26, for which ...200817729274
septic shock caused by sphingomonas paucimobilis bacteremia in a patient with hematopoietic stem cell transplantation.sphingomonas paucimobilis is an aerobic gram-negative bacillus that causes a variety of infections in healthy as well as in immunocompromised individuals. the organism is usually susceptible to tetracycline, chloramphenicol, aminoglycosides, trimethoprim-sulfamethoxazole, and carbapenems. however, resistance to penicillins and the first-generation cephalosporins is commonly encountered. reported here is a patient with acute myeloid leukemia who developed s. paucimobilis bacteremia complicated by ...200817605729
biodegradation kinetics of select polycyclic aromatic hydrocarbon (pah) mixtures by sphingomonas paucimobilis epa505.many contaminated sites commonly have complex mixtures of polycyclic aromatic hydrocarbons (pahs) whose individual microbial biodegradation may be altered in mixtures. biodegradation kinetics for fluorene, naphthalene, 1,5-dimethylnaphthalene and 1-methylfluorene were evaluated in sole substrate, binary and ternary systems using sphingomonas paucimobilis epa505. the first order rate constants for fluorene, naphthalene, 1,5-dimethylnaphthalene, and 1-methylfluorene were comparable; yet monod para ...200817534722
evaluation of the airborne bacterial population in the periodically confined antarctic base concordia.the environmental airborne bacterial population in relation to human confinement was investigated over a period of 1 year in the concordia research station, which is located on the eastern antarctic plateau. the unique location of the station makes it suitable for different research domains such as glaciology, atmospheric sciences, astronomy, etc. furthermore, it is used as a test bed for long-duration spaceflights to study the physiologic and psychological adaptation to isolated environments. a ...200918972149
sphingomonas paucimobilis bloodstream infections associated with contaminated intravenous fentanyl.nationally distributed medications from compounding pharmacies, which typically adhere to less stringent quality-control standards than pharmaceutical manufacturers, can lead to multistate outbreaks. we investigated a cluster of 6 patients in a maryland hospital who had sphingomonas paucimobilis bloodstream infections in november 2007. of the 6 case-patients, 5 (83%) had received intravenous fentanyl within 48 hours before bacteremia developed. cultures of unopened samples of fentanyl grew s. pa ...200919116043
water-borne sphingomonas paucimobilis epidemic in an intensive care unit. 200919232740
[effect of the inoculant strain sphingomonas paucimobilis 20006fa on the bacterial composition of a phenanthrene-degrading consortium].the effect of the inoculant strain sphingomonas paucimobilis 20006fa on the bacterial composition of a phenanthrene-degrading consortium obtained from a pristine soil in sequencing batch cultures was studied. inoculated (f200+1) and non-inoculated (f200) phenanthrene-degrading consortia, were obtained. bacterial diversity of consortia was studied at cultivable (phenotype and genotype characterization) and non-cultivable (pcr-dgge) levels. during the successive cultures, a loss in the phenanthren ...200919623894
microbial conversion of glucose to a novel chemical building block, 2-pyrone-4,6-dicarboxylic acid.2-pyrone-4,6-dicarboxylic acid (pdc) is a catabolic intermediate in sphingobium sp. syk-6 (previously characterized as sphingomonas paucimobilis syk-6), which is a degrader of lignin-derived aromatic compounds. recently, pdc has been also characterized as a novel starting material for several potentially useful synthetic polymers. in a previous study, we constructed a biosynthetic system in which pdc was generated efficiently from a chemically synthesized compound, protocatechuate. in order to d ...200919281857
the external aldimine form of serine palmitoyltransferase: structural, kinetic, and spectroscopic analysis of the wild-type enzyme and hsan1 mutant mimics.sphingolipid biosynthesis begins with the condensation of l-serine and palmitoyl-coa catalyzed by the plp-dependent enzyme serine palmitoyltransferase (spt). mutations in human spt cause hereditary sensory autonomic neuropathy type 1, a disease characterized by loss of feeling in extremities and severe pain. the human enzyme is a membrane-bound hetereodimer, and the most common mutations are located in the enzymatically incompetent monomer, suggesting a "dominant" or regulatory effect. the molec ...200919376777
distribution and biodegradation of polycyclic aromatic hydrocarbons in contaminated sites of hisar (india).fifty-two soil samples were collected from various location of the hisar city. these samples were analysed for six polycyclic aromatic hydrocarbons (naphthalene, acenaphthene, phenanthrene, anthracene, fluoranthene, and pyrene). total mean concentration of six pahs varied from 51.79 to 148.82 mg kg(-1) dry weight of the soil, pah concentration was higher in soil samples from local auto market while lower concentration was recorded in agricultural soil. effect ofph (5.0 to 9.0), temperature (20 d ...200919405388
sphingomonas paucimobilis bacteraemia and septic arthritis in a diabetic patient presenting with septic pulmonary emboli.sphingomonas paucimobilis, a yellow-pigmented, aerobic, glucose non-fermenting, gram-negative bacillus, is a rare cause of human infection normally associated with immunocompromised hosts. we report a case of bacteraemia and septic arthritis in a 47-year-old diabetic man who presented with septic pulmonary emboli due to s. paucimobilis. the patient had an initial presentation of fever, right knee pain, coughing, dyspnoea and chest pain. the infection was treated successfully by surgical debridem ...200919528166
evaluation of antibacterial activity, phenol and flavonoid contents of thespesia populnea flower extracts.flavonoids which were reported as having many pharmacological activities, antimicrobial, antioxidant, cytotoxic, chemoprevention activities and they possess strong antiproliferative effects related to inhibition of cell cycle progression and apoptosis induction. on the basis of this thespesia populnea (l.) sol. ex correa (family-malvaceae) was selected and it is having the major composition of flavonoids and the antibacterial activity of methanolic extract of thespesia populnea flowers was inves ...200919553175
bromhidrosis induced by sphingomonas paucimobilis: a case report.bromhidrosis is a clinical disorder characterized by excessive or abnormal foul axillary odour due to the interaction of apocrine glands with micro-organisms which causes a serious personal and social handicap for affected people. we present the case of a 50-year-old caucasian female with bromhidrosis. the patient referred that this symptom had begun two months previously. her past treatments included antibacterial soap, topical antibacterial agents and perfumes, but none of these relieved the p ...200919822101
intestinal obstruction associated with chronic peritonitis caused by sphingomonas paucimobilis.we describe a very rare case of chronic peritonitis with secondary adhesive intestinal obstruction caused by sphingomonas paucimobilis in a healthy 28-year-old chinese man. this bacillus has not been described as a cause of spontaneous peritonitis in healthy people. it was an asymptomatic, generalized, and slow-growing peritonitis causing peritoneal adherens and at the end intestinal occlusion that needed surgical adhesiolysis.200926192291
anticoagulating activities of low-molecular weight fuco-oligosaccharides prepared by enzymatic digestion of fucoidan from the sporophyll of korean undaria pinnatifida.in spite of their potential as biologically active compounds, the high molecular mass and viscous natures of fucoidans have hampered their applications especially as a therapeutic agent. herein the fucoidan-degrading enzyme activities were partially purified from the cultured cells of sphingomonas paucimobilis pf-1 mainly by ammonium sulfate precipitation. this enzyme preparation degraded fucoidans from the korean undaria pinnatifida sporophyll into several low-molecular weight fuco-oligosacchar ...201020191353
sphingomonas paucimobilis bacteremia in humans: 16 case reports and a literature review.sphingomonas paucimobilis is a glucose-nonfermenting gram-negative bacillus that is widely distributed in both natural environment and hospitals. various infections in humans have been reported, but most have been limited to sporadic case reports. the aim of this study was to describe the clinical characteristics and manifestations of s. paucimobilis bacteremia. we also reviewed the literature on s. paucimobilis bacteremia.201020434121
sphingomonas paucimobilis: a persistent gram-negative nosocomial infectious organism.non-fermenting gram-negative bacilli create a significant problem in clinical settings, being the most widespread cause of nosocomial infections. they are opportunistic pathogens that take advantage of underlying conditions and diseases. sphingomonas paucimobilis, a non-fermenting gram-negative bacillus, is regarded as of minor clinical significance; however, many instances of infections with this organism can be found in the literature. infections include bacteraemia/septicaemia caused by conta ...201020434794
inhibition of the plp-dependent enzyme serine palmitoyltransferase by cycloserine: evidence for a novel decarboxylative mechanism of inactivation.cycloserine (cs, 4-amino-3-isoxazolidone) is a cyclic amino acid mimic that is known to inhibit many essential pyridoxal 5'-phosphate (plp)-dependent enzymes. two cs enantiomers are known; d-cycloserine (dcs, also known as seromycin) is a natural product that is used to treat resistant mycobacterium tuberculosis infections as well as neurological disorders since it is a potent nmda receptor agonist, and l-cycloserine (lcs) is a synthetic enantiomer whose usefulness as a drug has been hampered by ...201020445930
detecting variants with metabolic design, a new software tool to design probes for explorative functional dna microarray development.microorganisms display vast diversity, and each one has its own set of genes, cell components and metabolic reactions. to assess their huge unexploited metabolic potential in different ecosystems, we need high throughput tools, such as functional microarrays, that allow the simultaneous analysis of thousands of genes. however, most classical functional microarrays use specific probes that monitor only known sequences, and so fail to cover the full microbial gene diversity present in complex envi ...201020860850
comparison of maldi tof with conventional identification of clinically relevant bacteria.a completely new approach to diagnose microbial agents at least one day earlier based on mass spectrometric analysis becomes possible in the microbiology laboratory: maldi tof: matrix-assisted laser desorption/ionisation time-of-flight. comparison between results of the new procedure with those obtained by conventional testing is mandatory.201020924806
use of reporter-gene based bacteria to quantify phenanthrene biodegradation and toxicity in soil.a phenanthrene-degrading bacterium, sphingomonas paucimobilis epa505 was used to construct two fluorescence-based reporter strains. strain d harboring gfp gene was constructed to generate green fluorescence when the strain started to biodegrade phenanthrene. strain s possessing gef gene was designed to die once phenanthrene biodegradation was initiated and thus to lose green fluorescence when visualized by a live/dead cell staining. confocal laser scanning microscopic observation followed by ima ...201021093134
application of the mixture design to optimise the formulation of active consortia to decolorize azo-dye methyl red.with the aid of analysis software (minitab 14.0), the formulation of pure culture in mineral salts medium (msm) can be optimized for several responses and the best formulation can be obtained. the influence of the different mixtures of three strains in the pure culture in msm on the flavor components in decolorization of methyl red (with initial total cell density fixed at od600 = 1 and in addition of 750 ppm of dye) was studied using equilateral triangle diagram and mixture experimental design ...201021123913
usefulness of platelet (plt) pathogen inactivation technology: a case of sphingomonas paucimobilis inactivation in an apheresis plt unit. 201021182632
study of the degradation activity and the strategies to promote the bioavailability of phenanthrene by sphingomonas paucimobilis strain 20006fa.the present study describes the phenanthrene-degrading activity of sphingomonas paucimobilis 20006fa and its ability to promote the bioavailability of phenanthrene. s. paucimobilis 20006fa was isolated from a phenanthrene-contaminated soil microcosm. the strain was able to grow in liquid mineral medium saturated with phenanthrene as the sole carbon source, showing high phenanthrene elimination (52.9% of the supplied phenanthrene within 20 days). the accumulation of 1-hydroxy-2-naphthoic acid and ...201019609598
gellan gum: a new biomaterial for cartilage tissue engineering applications.gellan gum is a polysaccharide manufactured by microbial fermentation of the sphingomonas paucimobilis microorganism, being commonly used in the food and pharmaceutical industry. it can be dissolved in water, and when heated and mixed with mono or divalent cations, forms a gel upon lowering the temperature under mild conditions. in this work, gellan gum hydrogels were analyzed as cells supports in the context of cartilage regeneration. gellan gum hydrogel discs were characterized in terms of mec ...201019658177
[sphingomonas paucimobilis infection with underlying submandibular sialolithiasis].sphingomonas paucimobilis (s. paucimobilis), is a gram-negative, aerobic, non-fermentative, oxidase (+) and catalase (+) bacterium. although s. paucimobilis is isolated very rarely, it can cause both nosocomial and community-acquired infections. a patient admitted to our clinic had a complaint of swelling in the right mandibular region and pain increasing while eating for the previous week. bimanual palpation revealed a painful swelling of 1x1x1 cm in size inside the right wharton's duct. via ma ...201121303318
a rare case of peripartum endogenous bacterial endophthalmitis.we describe the case of a healthy, pregnant female who developed endogenous endophthalmitis at the time of delivery, and discuss the possible mechanism of infection and the management of this case. a 26-year-old asian woman presented with a 3-week history of visual deterioration and pain in the right eye. there was no history of ocular trauma or surgery. the ocular symptoms developed one day after vaginal delivery of a healthy baby. the pregnancy had been uncomplicated until premature rupture of ...201121327678
application of the knowledge-based approach to strain selection for a bioaugmentation process of phenanthrene- and cr(vi)-contaminated soil.aims:  the objective of this study was to apply the knowledge-based approach to the selection of an inoculum to be used in bioaugmentation processes to facilitate phenanthrene degradation in phenanthrene- and cr(vi)-co-contaminated soils. methods and results:  the bacterial community composition of phenanthrene and phenanthrene- and cr(vi)-co-contaminated microcosms, determined by denaturing gradient gel electrophoresis analysis, showed that members of the sphingomonadaceae family were the predo ...201121518157
risk factors associated with sphingomonas paucimobilis infection.background: sphingomonas paucimobilis is rarely isolated from clinical specimens and it is associated with a great variety of infections. the aim of this study is to investigate the microbiological and clinical features of s paucimobilis infection in southern taiwan. methods: s paucimobilis isolates from the microbiology laboratory of chi-mei medical center and their relevant clinical data from october 2005 to october 2009 were retrospectively reviewed. results: a total of 55 patients with docum ...201121524965
synergistic effects and physiological responses of selected bacterial isolates from animal feed to four natural antimicrobials and two antibiotics.abstract in this study, 20 samples from three different sources of animal feed were investigated and six bacterial isolates were identified. the susceptibility of four natural antimicrobials, namely, eugenol, cinnamaldehyde, thymol, and carvacrol, against six of these isolates was determined. carvacrol and eugenol showed better inhibitory effects with larger zones of inhibition. the minimal inhibitory concentration for a range of antibiotics on the susceptibility of two isolates (namely, sphingo ...201121612425
crystal structure of h2o2-dependent cytochrome p450sp{alpha} with its bound fatty acid substrate: insight into the regioselective hydroxylation of fatty acids at the {alpha} position.cytochrome p450(sp+¦) (cyp152b1) isolated from sphingomonas paucimobilis is the first p450 to be classified as a h(2)o(2)-dependent p450. p450(sp+¦) hydroxylates fatty acids with high +¦-regioselectivity. herein we report the crystal structure of p450(sp+¦) with palmitic acid as a substrate at a resolution of 1.65 ôä½. the structure revealed that the c(+¦) of the bound palmitic acid in one of the alternative conformations is 4.5 ôä½ from the heme iron. this conformation explains the highly selec ...201121719702
outbreak of sphingomonas paucimobilis septicemia in a neonatal intensive care unit.we describe an outbreak of sphingomonas paucimobilis in 13 newborn infants with septicemia and septic shock. fifteen available isolates were obtained from patients and distilled water. pulsed-field gel electrophoresis results pointed out that there was a cross-transmission of s. paucimobilis in eleven patients but these types were not the same the isolate obtained from the distilled water. the outbreak was terminated by taking appropriate control measures for infection and change of source of di ...201121719938
fine tuning by human cd1e of lipid-specific immune responses.cd1e is a member of the cd1 family that participates in lipid antigen presentation without interacting with the t-cell receptor. it binds lipids in lysosomes and facilitates processing of complex glycolipids, thus promoting editing of lipid antigens. we find that cd1e may positively or negatively affect lipid presentation by cd1b, cd1c, and cd1d. this effect is caused by the capacity of cd1e to facilitate rapid formation of cd1-lipid complexes, as shown for cd1d, and also to accelerate their tur ...201121844346
sphingomonas paucimobilis bacteremia related to intravenous human immunoglobulin injections. 201122075255
Sphingomonas alpina sp. nov., a psychrophilic bacterium isolated from alpine soil.An aerobic, Gram-negative, motile, psychrophilic bacterium, designated strain S8-3T, was isolated from alpine soil. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain S8-3T was related to the genus Sphingomonas and had highest 16S rRNA gene sequence similarity with Sphingomonas oligophenolica S213T (98.0 %). 16S RNA gene sequence similarity between strain S8-3T and Sphingomonas paucimobilis ATCC 29837 T (the type species of the genus Sphingomonas) was 93.0 %. Strain S8-3T ...201121856979
[Endogenous postpartum panophthalmitis induced by sphingomonas paucimobili].Endogenous panophthalmitis is a rare eye disease with purulent necrosis of all the ocular structures. It is a rare but serious condition that occurs when bacteria cross the blood-ocular barrier and multiply within the eye. Although rare, endogenous panophthamitis is a potentially devastating intraocular infection resulting in a poor visual and anatomic prognosis. We present a 39-year-old woman, without any previous history, who developed a endogenous panophthalmitis in the left eye secondary to ...201122003763
the effect of sterile versus non-sterile tourniquets on microbiological colonisation in lower limb surgery.surgical tourniquets are commonplace in lower limb surgery. several studies have shown that tourniquets can be a potential source of microbial contamination but have not compared the use of sterile versus non-sterile tourniquets in the same procedures.201122041233
a rare cause of bacteremia in a pediatric patient with down syndrome: sphingomonas paucimobilis.sphingomonas paucimobilis, is a yellow-pigmented, aerobic, non fermentative, gram negative motile bacillus. s. paucimobilis which is widely found in nature and hospital environments rarely cause serious or life threatening infections. in this report, a case of hospital acquired bloodstream infection due to s. paucimobilis in a patient with down syndrome who was on treatment for presumed pneumonia is presented. a one year-old child patient who was a known case of down syndrome and had previously ...201121960744
food-borne bacteremic illnesses in febrile neutropenic children.bacteremia following febrile neutropenia is a serious complication in children with malignancies. preventive measures are currently targeted at antimicrobial prophylaxis, amelioration of drug-induced neutropenia, and nosocomial spread of pathogens, with little attention to community-acquired infections. a retrospective study was conducted at a pediatric oncology center during a 3-year period to identify probable cases of food-borne infections with bacteremia. twenty-one bacteremic illnesses affe ...201122184532
Enzymes involved in the biodegradation of hexachlorocyclohexane: A mini review.The scope of this paper encompasses the following subjects: (i) aerobic and anaerobic degradation pathways of ?-hexachlorocyclohexane (HCH); (ii) important genes and enzymes involved in the metabolic pathways of ?-HCH degradation; (iii) the instrumental methods for identifying and quantifying intermediate metabolites, such as gas chromatography coupled to mass spectrometry (GC-MS) and other techniques. It can be concluded that typical anaerobic and aerobic pathways of ?-HCH are well known for a ...201121992990
[bacterial profiles in conjunctival sac of middle-aged and elderly qiang with dry eyes and normal eyes].to compare the bacteria profiles in conjunctival sac of qiang aged 40 years and over between those with dry eyes and those with normal eyes.201122332548
analysis of the bacterial diversity existing on animal hide and wool: development of a preliminary pcr-restriction fragment length polymorphism fingerprint database for identifying isolates.twenty-one bacterial strains were isolated from imported cattle hide and rabbit wool using two types of media, nutrient broth, and nutrient broth with serum. the bacteria identified were brevibacillus laterosporus, leclercia adecarboxylata, peptococcus niger, bacillus circulans, raoultella ornithinolytica, bacillus subtilis, bacillus cereus, bacillus thermobacillus, bacillus choshinensis, bacillus sphaericus, acinetobacter haemolyticus, sphingomonas paucimobilis, bacillus thuringiensis, staphylo ...201223451394
structure and catalytic mechanism of ligi: insight into the amidohydrolase enzymes of cog3618 and lignin degradation.ligi from sphingomonas paucimobilis catalyzes the reversible hydrolysis of 2-pyrone-4,6-dicarboxylate (pdc) to 4-oxalomesaconate and 4-carboxy-2-hydroxymuconate in the degradation of lignin. this protein is a member of the amidohydrolase superfamily of enzymes. the protein was expressed in escherichia coli and then purified to homogeneity. the purified recombinant enzyme does not contain bound metal ions, and the addition of metal chelators or divalent metal ions to the assay mixtures does not a ...201222475079
diversity of bacteria nesting the plant cover of north sinai deserts, egypt.north sinai deserts were surveyed for the predominant plant cover and for the culturable bacteria nesting their roots and shoots. among 43 plant species reported, 13 are perennial (e.g. fagonia spp., pancratium spp.) and 30 annuals (e.g. bromus spp., erodium spp.). eleven species possessed rhizo-sheath, e.g. cyperus capitatus, panicum turgidum and trisetaria koelerioides. microbiological analyses demonstrated: the great diversity and richness of associated culturable bacteria, in particular nitr ...201225685397
a case of peritoneal dialysis-associated peritonitis caused by sphingomonas paucimobilis.sphingomonas paucimobilis is an aerobic gram-negative bacillus found in soil and water. knowledge regarding the role of this infectious agent is limited because it is rarely isolated from human material. furthermore, it is an unusual pathogen in cases of peritoneal dialysis (pd)-associated peritonitis. the clinical courses and outcomes of peritonitis caused by s. paucimobilis are variable. whereas some patients were cured with appropriate antibiotic therapy, others required catheter removal. cas ...201226877918
monitoring of antibiotic resistance in bacteria isolated from laboratory animals.the drug resistance of microorganisms isolated from laboratory animals never treated with antibiotics is being reported consistently, while the number of laboratory animals used in medicine, pharmacy, veterinary medicine, agriculture, nutrition, and environmental and health science has increased rapidly in korea. therefore, this study examined the development of antimicrobial resistance in bacteria isolated from laboratory animals bred in korea. a total of 443 isolates (7 species) containing 5 s ...201222787489
comparative study of bacterial status from conjunctival sac of the elder qiang minority and han people with dry eye in sichuan, china.to compare the status of bacteria in the conjunctival sac from the elder qiang minority and han people with dry eyes in sichuan, china.201222773985
removal of triphenylmethane dyes by bacterial consortium.a new consortium of four bacterial isolates (agrobacterium radiobacter; bacillus spp.; sphingomonas paucimobilis, and aeromonas hydrophila)-(cm-4) was used to degrade and to decolorize triphenylmethane dyes. all bacteria were isolated from activated sludge extracted from a wastewater treatment station of a dyeing industry plant. individual bacterial isolates exhibited a remarkable color-removal capability against crystal violet (50 mg/l) and malachite green (50 mg/l) dyes within 24 h. interestin ...201222623907
liver abscess caused by brevundimonas vesicularis in an immunocompetent patient.invasive infections caused by brevundimonas vesicularis are very rare in humans. we experienced an unusual case of liver abscess due to b. vesicularis in an immunocompetent young male. the patient was successfully treated by liver abscess drainage and with antimicrobial therapy of ceftriaxone followed by ampicillin/sulbactam. the organism found in the aspiration culture of the abscess material was initially reported, by using a vitek 2 system, as sphingomonas paucimobilis. however, later, b. ves ...201222767540
a biofilm model developed to investigate survival and disinfection of mycobacterium mucogenicum in potable water.water in healthcare environments can be a source for healthcare-associated infections (hai). however, information on the exposure risk to opportunistic pathogens in potable water distribution systems (pwds) is lacking. laboratory studies characterizing the interaction of opportunistic pathogens with biofilms are needed to understand their role in water systems within healthcare facilities. a stable, repeatable, pwds multi-species biofilm model comprising sphingomonas paucimobilis, methylobacteri ...201223082863
molecular and microscopic analysis of bacteria and viruses in exhaled breath collected using a simple impaction and condensing method.exhaled breath condensate (ebc) is increasingly being used as a non-invasive method for disease diagnosis and environmental exposure assessment. by using hydrophobic surface, ice, and droplet scavenging, a simple impaction and condensing based collection method is reported here. human subjects were recruited to exhale toward the device for 1, 2, 3, and 4 min. the exhaled breath quickly formed into tiny droplets on the hydrophobic surface, which were subsequently scavenged into a 10 µl rolling de ...201222848436
dynamic passive dosing for studying the biotransformation of hydrophobic organic chemicals: microbial degradation as an example.biotransformation plays a key role in hydrophobic organic compound (hoc) fate, and understanding kinetics as a function of (bio)availability is critical for elucidating persistence, accumulation, and toxicity. biotransformation mainly occurs in an aqueous environment, posing technical challenges for producing kinetic data because of low hoc solubilities and sorptive losses. to overcome these, a new experimental approach based on passive dosing is presented. this avoids using cosolvent for introd ...201222458885
septic arthritis caused by sphingomonas paucimobilis in an immunocompetent patient. 201222902983
sphingomonas starnbergensis sp. nov., isolated from a prealpine freshwater lake.a novel type of freshwater bacterium was isolated from the prealpine mesotrophic starnberger see (bavaria, southern germany). cells of strain 382(t) were gram-negative and rod-shaped, motile and creamy-white. the isolate was strictly aerobic, catalase- and oxidase-positive, and grew at ph values of 6-9 (optimum, ph 7) and temperatures of 10-37 °c (optimum, 28 °c). the genomic g+c content of strain 382(t) was 64.1 mol%. based on 16s rrna gene sequence analyses, strain 382(t) belongs to the family ...201322685105
postpartum fever in the presence of a fibroid: sphingomonas paucimobilis sepsis associated with pyomyoma.pyomyoma is a life-threatening complication of uterine leiomyoma. it may occur in post- menopausal women, during pregnancy and in the postpartum period. fever may be the only manifestation during the early stages of the disease. we detail the first reported case of postpartum pyomyoma-related sepsis due to sphingomonas paucimobilis, a gram-negative bacillus that is gaining recognition as an important human pathogen.201324308831
[septic monarthritis caused by sphingomonas paucimobilis].sphingomonas paucimobilis, formerly known as pseudomonas paucimobilis, is a rare cause of septic arthritis and is therefore regarded as being of minor clinical interest in rheumatological diagnostics. in this connection the yellow pigmented, aerobic, glucose non-fermenting, gram negative bacillus is usually associated with immunocompromised patients. a case of septic arthritis in a 70-year-old man with chronic obstructive pulmonary disease (copd) initially presenting with right knee pain, swelli ...201323921948
sphingomonas paucimobilis infections in children: 24 case reports.sphingomonas paucimobilis is a causative agent of infection in immunocompromised patients, and healthcare-associated infection. although the infections associated with s.paucimobilis occurs rarely, it has been encountered with increasing frequency in clinical settings. in the current study we reported clinical features of the children with s.paucimobilis infection, and the antimicrobial susceptibilities of the isolated strains among the patients. this study was conducted in dr. behçet uz childre ...201323795278
an insight into the emerging exopolysaccharide gellan gum as a novel polymer.the microbial exopolysaccharides are water-soluble polymers secreted by microorganisms during fermentation. the biopolymer gellan gum is a relatively recent addition to the family of microbial polysaccharides that is gaining much importance in food, pharmaceutical and chemical industries due to its novel properties. it is commercially produced by c.p. kelco in japan and the usa. this article presents a critical review of the available information on the gum synthesized by sphingomonas paucimobil ...201323499110
[sphingomonas paucimobilis]. 201323450409
sphingomonas paucimobilis osteomyelitis in an immunocompetent patient. a rare case report and literature review.sphingomonas paucimobilis occurs widely both in natural and nosocomial environments, including hospital water systems, respiratory therapy equipment, and laboratory instruments. it is an opportunistic pathogen that rarely causes infections in humans. among s. paucimobilis nosocomial infections, osteomyelitis is particularly rare. almost all infections occur in patients with comorbidities such as malignancy, immunosuppressant therapy, diabetes mellitus and acquired immunodeficiency syndrome. we p ...201324177306
the pyridoxal 5'-phosphate (plp)-dependent enzyme serine palmitoyltransferase (spt): effects of the small subunits and insights from bacterial mimics of human hlcb2a hsan1 mutations.the pyridoxal 5'-phosphate (plp)-dependent enzyme serine palmitoyltransferase (spt) catalyses the first step of de novo sphingolipid biosynthesis. the core human enzyme is a membrane-bound heterodimer composed of two subunits (hlcb1 and hlcb2a/b), and mutations in both hlcb1 (e.g., c133w and c133y) and hlcb2a (e.g., v359m, g382v, and i504f) have been identified in patients with hereditary sensory and autonomic neuropathy type i (hsan1), an inherited disorder that affects sensory and autonomic ne ...201324175284
a new strategy to enhance gellan production by two-stage culture in sphingomonas paucimobilis.the effects of different initial sucrose concentrations and temperatures on gellan biosynthesis by sphingomonas paucimobilis atcc 31461 were investigated. lower sucrose concentrations and higher temperatures were favorable for cell growth. higher sucrose concentrations and lower temperatures promoted gellan production but retarded cell growth. based on these results, a two-stage culture strategy was developed to improve gellan production. during the first 24 h, s. paucimobilis was cultured in a ...201323987418
the draft genome sequence of sphingomonas paucimobilis strain her1398 (proteobacteria), host to the giant pau phage, indicates that it is a member of the genus sphingobacterium (bacteroidetes).the draft genome sequence of sphingomonas paucimobilis host index number (her) 1398, host of the giant pau phage isolated from silk moths (bombyx mori), indicates that this isolate belongs within the genus sphingobacterium. we suggest that sphingomonas paucimobilis strain her1398 be reclassified as sphingobacterium paucimobilis strain her1398.201323929486
engineering bacteria for bioremediation of persistent organochlorine pesticide lindane (γ-hexachlorocyclohexane).strategies were designed for bioremediation of the highly persistent toxic pesticide γ-hexachlorocyclohexane (γ-hch) or lindane from the environment. lindane caused the loss of stress-protective chaperone groel, and inhibited photosynthesis, respiration and nitrogen-fixation in anabaena, resulting in growth arrest. to alleviate lindane toxicity, the lina2 gene, encoding hch dehydrochlorinase from sphingomonas paucimobilis b90, was knocked-in at an innocuous locus in anabaena genome and over-expr ...201324135568
an evaluation of microbial and chemical contamination sources related to the deterioration of tap water quality in the household water supply system.the predominant microorganisms in samples taken from shower heads in residences in the korean city "n" were stenotrophomonas maltophilia, sphingomonas paucimobilis, acidovorax temperans, and microbacterium lacticum. legionella was not detected in this case. the volatile organic compounds (vocs) vinylacetate, nn-dma, cis-1,2-dichloroethylene, epichlorohydrin, and styrene were measured in five types of plastic pipes: pvc, pb, pp, pe, and cpvc. the rate of multiplication of the heterotrophic plate ...201324018837
community acquired bacteremia by sphingomonas paucimobilis: two rare case reports.s.paucimobilis has a diverse nutritional substrate spectrum and found in both environmental and hospital settings. sphingomonas paucimobilis is rarely isolated from clinical specimen. this low virulence organism since has been reported to cause a variety of diseases since 1979. it has been reported to be associated with both community acquired and nosocomial diseases including bacteremia, catheter related sepsis, diarrhoeal diseases, peritonitis, meningitis, cutaneous infections, endopthalmitis, ...201324551684
characterizing the promiscuity of ligab, a lignin catabolite degrading extradiol dioxygenase from sphingomonas paucimobilis syk-6.ligab from sphingomonas paucimobilis syk-6 is the only structurally characterized dioxygenase of the largely uncharacterized superfamily of type ii extradiol dioxygenases (edo). this enzyme catalyzes the oxidative ring-opening of protocatechuate (3,4-dihydroxybenzoic acid or pca) in a pathway allowing the degradation of lignin derived aromatic compounds (ldacs). ligab has also been shown to utilize two other ldacs from the same metabolic pathway as substrates, gallate, and 3-o-methyl gallate; ho ...201323977959
nonfermenting gram-negative bacilli other than pseudomonas aeruginosa and acinetobacter spp. causing respiratory tract infections in a tertiary care center.nonfermenting gram-negative bacilli have emerged as important healthcare-associated pathogens. it is important to correctly identify all clinically significant nonfermenting gram-negative bacilli considering the intrinsic multidrug resistance exhibited by these bacteria.201324672175
sanitization of an automatic reverse-osmosis watering system: removal of a clinically significant biofilm.during environmental monitoring of our institution's rodent watering systems, one vivarium was found to have high bacterial loads in the reverse-osmosis (ro) automatic water system. these findings prompted evaluation of the entire ro water production and distribution system. investigation revealed insufficient rack and ro system sanitization, leading to heavy biofilm accumulation within the system. approximately 2 wk after discovery in the water system, one of the bacterial organisms isolated in ...201323562105
prevalence of antibacterial resistant bacterial contaminants from mobile phones of hospital inpatients.mobile phones contaminated with bacteria may act as fomites. antibiotic resistant bacterial contamination of mobile phones of inpatients was studied. one hundred and six samples were collected from mobile phones of patients admitted in various hospitals in jazan province of saudi arabia. eighty-nine (83.9%) out of 106 mobile phones were found to be contaminated with bacteria. fifty-two (49.0%) coagulase-negative staphylococcus, 12 (11.3%) staphylococcus aureus, 7 (6.6%) enterobacter cloacae, 3 ( ...201425292217
in silico detection of virulence gene homologues in the human pathogen sphingomonas spp.there is an ongoing debate about the clinical significance of sphingomonas paucimobilis as a virulent bacterial pathogen. in the present study, we investigated the presence of different virulence factors and genes in sphingomonas bacteria. we utilized phylogenetic, comparative genomics and bioinformatics analysis to investigate the potentiality of sphingomonas bacteria as virulent pathogenic bacteria. the 16s ribosomal rna gene (16s rdna) phylogenetic tree showed that the closest bacterial taxon ...201425574122
prevalence of antibacterial resistant bacterial contaminants from mobile phones of hospital inpatients.mobile phones contaminated with bacteria may act as fomites. antibiotic resistant bacterial contamination of mobile phones of inpatients was studied. one hundred and six samples were collected from mobile phones of patients admitted in various hospitals in jazan province of saudi arabia. eighty-nine (83.9%) out of 106 mobile phones were found to be contaminated with bacteria. fifty-two (49.0%) coagulase-negative staphylococcus, 12 (11.3%) staphylococcus aureus, 7 (6.6%) enterobacter cloacae, 3 ( ...201428156258
enhanced gellan gum production by hydrogen peroxide (h2o 2) induced oxidative stresses in sphingomonas paucimobilis.in this study, the effect of h2o2-induced oxidative stress on gellan gum production and cell growth were investigated. gellan gum production was improved and cell growth was inhibited by h2o2. a multiple h2o2 stresses with different concentrations were developed to optimize gellan gum production. a maximal gellan gum yield (22.52 g/l), which was 35.58 % higher than the control, was observed with 2, 2, 3, 4 mmol/l h2o2 added at 6, 12, 18, 24 h, respectively. moreover, udp-glucose pyrophosphorylas ...201423925529
Refresh
firstprev
12345
nextlast
Displaying items 301 - 400 of 452