Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| genomic classification of new betanodavirus isolates by phylogenetic analysis of the coat protein gene suggests a low host-fish species specificity. | viral encephalopathy and retinopathy is a devastating disease that causes neurological disorders and high mortality in a large number of cultivated marine fish species around the world. it is now established that several viral strains classified in the genus betanodavirus of the family nodaviridae are the aetiological agents of this disease. betanodaviruses can be classified into four genotypes based on the coat protein gene sequence. here, the coat protein genes of the three major strains isola ... | 2004 | 15448371 |
| effect of dietary iron overload on photobacterium damselae ssp. piscicida pathogenicity in sea bass, dicentrarchus labrax (l.). | 2004 | 15509262 | |
| glutathione protects heavy metal-induced inhibition of hepatic microsomal ethoxyresorufin o-deethylase activity in dicentrarchus labrax l. | the in vitro effects of chromium (cr(vi)), copper (cu2+), iron (fe2+), mercury (hg2+), and zinc (zn2+) were assessed on liver microsomal ethoxyresorufin o-deethylase (erod) activity from a sea bass (dicentrarchus labrax l.) preexposed under laboratory conditions to 2.7 microm beta-naphthoflavone. the reduced glutathione (gsh) protection potential against heavy metal effects was also studied. the heavy metal concentration ranges used for this study were as follows: 10 pm-5 mm cr(vi), 10 pm-100 mi ... | 2004 | 15223263 |
| nodavirus infections in israeli mariculture. | viral encephalopathy and retinopathy (ver) infections were diagnosed in five fish species: epinephelus aeneus, dicentrarchus labrax, sciaenops ocellatus, lates calcarifer and mugil cephalus cultured on both the red sea and mediterranean coasts of israel during 1998-2002. spongiform vacuolation of nervous tissue was observed in histological sections of all examined species. with transmission electron microscopy, paracrystalline arrays and pieces of membrane-associated non-enveloped virions measur ... | 2004 | 15291788 |
| production and characterization of recombinantly derived peptides and antibodies for accurate determinations of somatolactin, growth hormone and insulin-like growth factor-i in european sea bass (dicentrarchus labrax). | a specific radioimmunoassay (ria) for european sea bass (dicentrarchus labrax) growth hormone (gh) was developed and validated. for this purpose, a stable source of gh was produced by means of recombinant dna technology in a bacteria system. the identity of the purified protein (ion exchange chromatography) was demonstrated by western blot and a specific gh antiserum was raised in rabbit. in western blot and ria system, this antiserum recognized specifically native and recombinant gh, and it did ... | 2004 | 15560873 |
| occurrence of scuticociliatosis in olive flounder paralichthys olivaceus by phiasterides dicentrarchi (ciliophora: scuticociliatida). | in the course of identifying scuticociliates recently obtained from systemically infected olive flounder paralichthys olivaceus in korea, we found a scuticociliate species whose small subunit ribosomal rna (ss rrna) gene was not amplified by species-specific primers previously designed for uronema marinum and pseudocohnilembus persalinus. by studying morphological characteristics of wet-mounted and stained specimens, we identified the species as philasterides dicentrarchi, which has been reporte ... | 2004 | 15672879 |
| modelling of fish interleukin-1 and its receptor. | this paper presents original data regarding: the three-dimensional modelling of rainbow trout (oncorhynchus mykiss) and sea bass (dicentrarchus labrax) il-1beta, the modelling of trout il-1 receptor type i (il-1ri) and the modelling of trout il-1beta bound to its receptor. the 3d models of trout and sea bass il-1beta molecules were predicted by comparison with those already available of human and mouse, and, in both cases, a structure consisting of a beta-trefoil fold was obtained, a motif well ... | 2004 | 15062642 |
| development and linkage relationships for new microsatellite markers of the sea bass (dicentrarchus labrax l.). | twenty-eight polymorphic microsatellites were isolated from the sea bass, dicentrarchus labrax, using a microsatellite enrichment protocol and selective hybridization with oligonucleotide probes. analysis for these markers and 11 recently described microsatellites of d. labrax found linkage between 26 loci and revealed eight linkage groups. | 2004 | 14731231 |
| cloning and expression of gonadotropin-releasing hormone receptor in the brain and pituitary of the european sea bass: an in situ hybridization study. | a full-length cdna encoding a gnrh receptor (gnrh-r) has been obtained from the pituitary of the european sea bass, dicentrarchus labrax. the complete cdna is 1814 base pairs (bp) in length and encodes a protein of 416 amino acids. the 5' utr and 3' utr are 239 bp and 324 bp in size, respectively. the expression sites of this gnrh-r were studied in the brain and pituitary of sea bass by means of in situ hybridization. a quantitative analysis of the expression of the gnrh-r gene along the reprodu ... | 2004 | 14724132 |
| immunoglobulin protein and gene transcripts in ovarian follicles throughout oogenesis in the teleost dicentrarchus labrax. | transfer of immunoglobulins (igm-like) from the female to the teleost embryo has been demonstrated but mechanisms of uptake into and storage within the eggs remain to be clarified. the monoclonal antibody dlig3 against dicentrarchus labrax ig light chain revealed an active role of both follicle cells and oocytes in the ig uptake. the primordial follicular cells showed dlig3 immunoreactivity even at a pre-vitellogenetic stage. early vitellogenetic oocytes (lipid vesicle stages) had dlig3 staining ... | 2004 | 14658064 |
| adenosine triphosphate concentration and beta-d-glucuronidase activity as indicators of sea bass semen quality. | the most common parameters used to evaluate sperm quality are motility rate and duration and fertilization ability. in this study, chemical and biochemical parameters of sea bass (dicentrarchus labrax) sperm were investigated to find an alternative method for evaluating sperm fertilization ability before and after cryopreservation. the biochemical and chemical analyses were performed with fresh and frozen-thawed sperm and seminal plasma. to cryopreserve sperm, 250-microl straws were used. fertil ... | 2004 | 14960478 |
| binding characteristics and daily rhythms of melatonin receptors are distinct in the retina and the brain areas of the european sea bass retina (dicentrarchus labrax). | melatonin is synthesized, with a circadian rhythm, in the pineal organ of vertebrates, high levels being produced during the scotophase and low levels during the photophase. the retina also produces melatonin, although in the case of the european sea bass, its secretion pattern appears to be inverted. in the study described here, radioreceptor assay techniques were used to characterize the melatonin binding sites, their regional distribution and their daily variations. brain and retina membrane ... | 2004 | 15542079 |
| molecular characterization of three peroxisome proliferator-activated receptors from the sea bass (dicentrarchus labrax). | peroxisome proliferator-activated receptors (ppar) are nuclear hormone receptors that control the expression of genes involved in lipid homeostasis in mammals. we searched for ppar in sea bass, a marine fish of particular interest to aquaculture, after hypothesizing that the physiological and molecular processes that regulate lipid metabolism in fish are similar to those in mammals. here, we report the identification of complementary dna and corresponding genomic sequences that encode three dist ... | 2004 | 15726823 |
| modulation of the activity of sea bass (dicentrarchus labrax) head-kidney macrophages by macrophage activating factor(s) and lipopolysaccharide. | the aim of this study was to establish the requirements for macrophage activating factor (maf) production by sea bass head-kidney leucocytes and the kinetics of macrophage activation when exposed to maf-containing supernatants and/or lipopolysaccharide (lps), a known macrophage stimulant. maf activity was found in culture supernatants of total head-kidney leucocytes pulsed with 5 microg ml(-1)con a, 5 or 10 ng ml(-1)pma and 100 ng ml(-1)calcium ionophore, or 10 microg ml(-1)con a alone, as asses ... | 2004 | 15123313 |
| melatonin binding sites in the brain of european sea bass (dicentrarchus labrax). | characteristics, day-night changes, guanosine 5'-o-(3-thiotriphosphate) (gtpgammas) modulation, and localization of melatonin binding sites in the brain of a marine teleost, european sea bass dicentrarchus labrax, were studied by radioreceptor assay using 2-[(125)i]iodomelatonin as a radioligand. the specific binding to the sea bass brain membranes was rapid, stable, saturable and reversible. the radioligand binds to a single class of receptor site with the affinity (kd) of 9.3 +/-0.6 pm and tot ... | 2004 | 15118230 |
| fatty acid pattern, oxidation product development, and antioxidant loss in muscle tissue of rainbow trout and dicentrarchus labrax during growth. | the levels of hydrophilic, lipophilic, and enzymatic antioxidants, the oxidative damage to lipids and proteins, and the fatty acid patterns of triglyceride and phospholipid fractions were assayed in fresh muscle tissue of rainbow trouts (oncorhynchus mykiss) and sea basses (dicentrarchus labrax) during aging, to investigate the correlation between oxidative stress and aging processes in fish. the present studies suggests that lipid peroxidation and accumulation of oxidized proteins during in viv ... | 2004 | 15113162 |
| beta-naphthoflavone alters normal plasma levels of vitellogenin, 17 beta-estradiol and luteinizing hormone in sea bass broodstock. | a variety of environmental pollutants exhibit antiestrogenic properties, i.e. these compounds antagonize estrogen-dependent processes in their target tissues. this is the case with beta-naphthoflavone (beta nf), a compound that is known for its ability to bind to the arylhydrocarbon receptor (ahr) and thereby to induce cytochrome p4501a (cyp1a) expression. in previous studies, we have shown that beta nf antagonizes the 17 beta-estradiol (e2)-mediated production of vitellogenin (vtg) in cultured ... | 2004 | 15084410 |
| bioavailability of oxytetracycline in sea bass, dicentrarchus labrax (l.). | 2004 | 15009254 | |
| comment on "biomagnification study on organochlorine compounds in marine aquaculture: the sea bass (dicentrarchus labrax) as a model". | 2004 | 14998046 | |
| dietary tag source and level affect performance and lipase expression in larval sea bass (dicentrarchus labrax). | the influence of dietary tag source (fish oil, triolein, and coconut oil) and level (7.5 and 15% of the diet) on growth, lipase activity, and mrna level was studied in sea bass larvae, from mouth opening until day 24 and from day 37 to 52. fish oil and triolein induced better growth in both experiments, this being significant at a higher dietary level. coconut oil significantly decreased growth at the higher level, possibly as the result of an excessive supply of medium-chain tag. growth was not ... | 2004 | 15506240 |
| a model for acute iron overload in sea bass (dicentrarchus labrax l.). | evaluation of several parameters involved in iron metabolism was carried out after intraperitoneal (i.p.) injection with iron dextran (idx) in sea bass (dicentrarchus labrax l.). after treatment, a rapid mobilization of idx from the peritoneal cavity to other organs was observed. this was followed by a modification of normal peripheral blood iron parameters. total iron (ti) and transferrin saturation (ts) rose rapidly, to 4.14 microg/ml and 83.7%, respectively, on day 3. in contrast, unsaturated ... | 2004 | 15479557 |
| sensory evaluation of sea bass (dicentrarchus labrax l.) fed two diets differing in fat content. | 2004 | 15372963 | |
| vitamin e in early stages of sea bass (dicentrarchus labrax) development. | this study reports titration of vitamin e levels in the sea bass (dicentrarchus labrax) using high-pressure liquid chromatography. the first part of the work is devoted to vitamin e detection in: (1) plasma of maturing females and males characterized by different body sizes; (2) seminal fluid and eggs; and (3) developing embryos of sea bass fed with vitamin e. in the second part of the study, variations of vitamin e levels during larval development are analyzed. the results show a direct correla ... | 2004 | 15369832 |
| juvenile sea bass biotransformation, genotoxic and endocrine responses to beta-naphthoflavone, 4-nonylphenol and 17 beta-estradiol individual and combined exposures. | juvenile sea bass, dicentrarchus labrax l., were exposed during 2, 4, 8, and 24 h to 0.9 microm beta-naphthoflavone (bnf), 131 nm 17 beta-estradiol (e(2)), 4.05 microm 4-nonylphenol (np), as well as to bnf combined either to e(2) or np (maintaining the previous concentrations). liver cytochrome p450 content (p450), ethoxyresorufin-o-deethylase (erod), and glutathione s-transferase (gst) activities were measured in order to evaluate biotransformation responses. genotoxicity was assessed as erythr ... | 2004 | 15294438 |
| cloning, characterisation, and expression of three oestrogen receptors (eralpha, erbeta1 and erbeta2) in the european sea bass, dicentrarchus labrax. | three oestrogen receptor [er] subtypes have been described in teleost fish, namely eralpha, and two erbeta subtypes, called erbeta1 and erbeta2 (or erbeta and ergamma in atlantic croaker). their expression during embryonic development and gonadal growth has evoked interest in their potential role in sexual differentiation and gonadal development in fish. we cloned three oestrogen receptors from adult liver (sb-eralpha cdna) and ovary (partial sb-erbeta1 and sb-erbeta2 cdnas) of the european sea ... | 2004 | 15279912 |
| cloning, sequence analysis, tissue distribution, and sex-specific expression of the neural form of p450 aromatase in juvenile sea bass (dicentrarchus labrax). | the teleost brain is characterized by exceptionally high levels of aromatase, the enzyme that converts androgens into estrogens, and by its continuous growth throughout life. gonadal estrogens have been implicated in sex differentiation and the control of reproduction in adult fish, but the role of neural estrogens during early development is far from clear. the present study describes the isolation and characterization of the cdna sequence from brain aromatase (p450aromb) in the european sea ba ... | 2004 | 15149730 |
| rearing density influences the expression of stress-related genes in sea bass (dicentrarchus labrax, l.). | the worldwide decline of ocean fisheries stocks has provided a rapid growth in fish farming and the problems connected to animal welfare in aquaculture gained importance. in this context, we have looked for molecular markers among those genes whose expression could reasonably result modified by the different farming conditions. with this purpose, we have evaluated, in liver and brain of sea basses, grown for 3 months at different biomass density (<10, 80 and 100 kg/m3), the expression of those g ... | 2004 | 15474294 |
| oral administration of melatonin reduces food intake and modifies macronutrient selection in european sea bass (dicentrarchus labrax, l.). | seasonal and circadian changes have been observed in dietary selection and feeding behavior of fish. it is known that the light-dark cycle is the principal mediator of the production of pineal-derived melatonin in fish, and also that the digestive tract synthesizes and secretes melatonin and indeed is the principal extrapineal source of this secretor product, suggesting that melatonin could be involved in the processes of feeding and/or digestion. fish are capable of regulating their energy inta ... | 2004 | 15230867 |
| formation of the egg envelope of a teleost, dicentrarchus labrax (l.): immunochemical and cytochemical detection of multiple components. | the formation of the egg envelope in a teleost, dicentrarchus labrax (l.), was analysed at histological and ultrastructural level. the sequential deposition of three main layers (z1, z2 and z3) constitutes the extracellular matrix throughout oocyte development. various findings indicate that these subunits are biochemically distinct: (1) periodate- and phosphotungstic acid-reactive carbohydrates are obviously detected only in the z1, that constitutes the initial deposit of the egg envelope in ea ... | 2004 | 14991400 |
| analysis of the sox gene family in the european sea bass (dicentrarchus labrax). | sox (sry-related genes containing a hmg box) genes encode a family of transcription factors that are involved in a variety of developmental processes including sex determination. twenty sox genes are present in the genomes of humans and mice, but far less is known about the sox gene family in other vertebrate types. we have obtained clones representing the hmg boxes of twelve sox genes from european sea bass (dicentrarchus labrax), a fish species whose farming is complicated by a heavily skewed ... | 2004 | 14990224 |
| effect of photoperiod manipulation on the daily rhythms of melatonin and reproductive hormones in caged european sea bass (dicentrarchus labrax). | reproduction in fish is cyclical and timed to guarantee the survival of the offspring. seasonal variations in reproductive hormones of fish have been deeply investigated in fish over the last years. however, there are few studies regarding the daily changes in reproductive hormone profiles in teleosts. the aim of the present research was to investigate the effects of photoperiod manipulation on melatonin and reproductive hormones (pituitary sbgnrh, pituitary lh and plasma lh, testosterone [t], a ... | 2004 | 14980798 |
| occurrence of acth- and enkephalin-like peptides in the developing gut of dicentrarchus labrax l. | we carried out immunohistochemical tests in the developing gut of the sea bass dicentrarchus labrax to follow the appearance and distribution of the immunoreactivity (ir) to antibodies against pomc-derived, adrenocorticotropic hormone (acth), alpha-melanocyte stimulating hormone (alpha-msh) and beta-endorphin (beta-end), and against two enkephalins, with the aim to study a possible involvement of these molecules in the early neuro-immune-endocrine integration. our data show that ir to antibodies ... | 2004 | 14980793 |
| cardiac preload and venous return in swimming sea bass (dicentrarchus labrax l.). | cardiac preload (central venous pressure, p(cv), mean circulatory filling pressure (mcfp), dorsal aortic blood pressure (p(da)) and relative cardiac output (q) were measured in sea bass (dicentrarchus labrax) at rest and while swimming at 1 and 2 bl s(-1). mcfp, an index of venous capacitance and the upstream venous pressure driving the return of venous blood to the heart, was measured as the plateau in pcv during ventral aortic occlusion. compared with resting values, swimming at 1 and 2 bl s(- ... | 2005 | 15879073 |
| sea bass (dicentrarchus labrax) androgen receptor: cdna cloning, tissue-specific expression, and mrna levels during early development and sex differentiation. | androgens play key roles in vertebrate sex differentiation, gonadal maturation and reproductive behaviour and their actions are generally mediated through specific nuclear receptors. the present study describes the isolation, sequencing and characterization of the cdna encoding the androgen receptor (ar) in the european sea bass. ar was cloned from a sea bass testis cdna library and encoded a predicted protein of 767 residues, with a calculated molecular weight of 86.4 kda and a theoretical pi o ... | 2005 | 15878229 |
| quantification of a glucocorticoid receptor in sea bass (dicentrarchus labrax, l.) reared at high stocking density. | to assess the effects of environmental stress conditions on fish, the examination of a suite of biomarkers, including endocrine parameters, has been suggested. in teleosts, glucocorticoids, including cortisol and corticosterone, are known to mediate stress response. inside the cell, they bind to a high-affinity cytosolic glucocorticoid receptor (gr), which acts as ligand-dependent transcription factor to control and regulate gene expression. receptor number or affinity may directly influence the ... | 2005 | 16129569 |
| dietary protein source affects lipid metabolism in the european seabass (dicentrarchus labrax). | the study was undertaken to evaluate the effects of dietary protein sources on lipogenesis and fat deposition in a marine teleost, the european seabass (dicentrarchus labrax). four isonitrogenous (crude protein (cp, nx6.25), 44% dm) and isoenergetic (22-23 kj/g dm) diets were formulated to contain one of the following as the major protein source: fish meal (fm), one of two soy protein concentrates (spc) and corn gluten meal (cgm). apparent digestibility coefficients of the diets and raw ingredie ... | 2005 | 16087375 |
| maintenance of genetic differentiation across a transition zone in the sea: discordance between nuclear and cytoplasmic markers. | to investigate the origin and maintenance of the genetic discontinuity between atlantic and mediterranean populations of the common sea bass (dicentrarchus labrax) we analysed the genetic variation at a fragment of mitochondrial cytochrome b sequence for 18 population samples. the result were also compared with new or previously published microsatellite data. seven mitochondrial haplotypes and an average nucleotidic divergence of 0.02 between atlantic and mediterranean populations that matches a ... | 2005 | 15669962 |
| molecular markers for animal biotechnology: sea bass (dicentrarchus labrax, l.) hmg-coa reductase mrna. | modern technologies may improve fish production and quality and, at the same time, reduce environmental impact with benefits on the public perception of the industry. to be economically profitable, these modern technologies request an increase of rearing density that, however, could affect fish welfare. with the aim to search for molecular biomarkers to describe fish welfare, we have recently compared gene expression of sea bass farmed at different population densities by differential display ob ... | 2005 | 15656995 |
| lordotic vertebrae in sea bass (dicentrarchus labrax l.) are adapted to increased loads. | lordosis in fish is an abnormal ventral curvature of the vertebral column, accompanied by abnormal calcification of the afflicted vertebrae. incidences of lordosis are a major problem in aquaculture and often correlate with increased swimming activity. to understand the biomechanical causes and consequences of lordosis, we mapped the morphological changes that occur in the vertebrae of european sea bass during their development from larva to juvenile. our micro-ct analysis of lordotic and non-lo ... | 2005 | 15863108 |
| muscle cellularity at cranial and caudal levels of the trunk musculature of commercial size sea bass, dicentrarchus labrax (linnaeus, 1758). | in eight specimens of atlantic sea bass of commercial size (congruent with 350 g) muscle cellularity was studied at two selected sampling levels of the trunk axial musculature: caudal (anal opening) and cranial (fourth radius of the dorsal fin). the following parameters were quantified at both sampling levels: white muscle cross-sectional area, white muscle fibre diameter (900-1200 fibres), muscle fibre number and muscle fibre density. results showed a higher total cross-sectional area at crania ... | 2005 | 16159368 |
| genetic, endocrine, and environmental components of sex determination and differentiation in the european sea bass (dicentrarchus labrax l.). | the european sea bass (dicentrarchus labrax l.) is a differentiated gonochoristic marine teleost of the family moronidae (closely related to the hermaphrodites of the family serranidae), where many juvenile males exhibit intratesticular oocytes, suggesting a certain sexual lability. like most fish, the sea bass does not have recognizable heterochromosomes or sex-linked markers but there are clear parental effects on the sex ratios. the data available so far indicate that the proportion of female ... | 2005 | 15862554 |
| fish macronutrient selection through post-ingestive signals: effect of selective macronutrient deprivation. | recent reports describe teleosts as being able to regulate energy intake by selecting from pure macronutrient sources, although the regulatory mechanisms involved in this selection remain unknown. the aim of the present work was to determine the effect of selective macronutrient deprivation on energy regulation and macronutrient selection, using for this purpose carbohydrate (ch), protein (p), and fat (f) packaged separately into gelatin capsules, a method that prevents the diet chemosensory pro ... | 2005 | 15811401 |
| the polarisation of hair cells from the ear of the european bass (dicentrarchus labrax). | the polarisation of ciliary bundles on the macula of the saccule in the european bass (dicentrarchus labrax l) has been studied using a scanning electron microscope (sem). these data show that d. labrax possesses ciliary bundles arranged in four dichotomous quadrants with a standard orientation, comparable to hearing generalists from the order perciformes. the spacing between ciliary bundles was investigated in three size classes of fish, with the results indicating that the addition of receptor ... | 2005 | 15922641 |
| high-pressure effects on the proteolytic enzymes of sea bass (dicentrarchus labrax l.) fillets. | high-pressure processing is a nonthermal technique ensuring food product safety and enabling a longer shelf life. the purpose of this experiment was to evaluate the effect of high pressure on the main proteolytic enzymes involved in fish muscle degradation during storage. enzymes were extracted with sarcoplasmic proteins from dicentrarchus labrax sea bass white muscle. activity of cathepsins b, d, h, and l was quantified in protein extract, whereas calpain activity was evaluated after isolation ... | 2005 | 15884825 |
| differential freshwater adaptation in juvenile sea-bass dicentrarchus labrax: involvement of gills and urinary system. | the effects of long-term freshwater acclimatization were investigated in juvenile sea-bass dicentrarchus labrax to determine whether all sea-bass juveniles are able to live in freshwater and to investigate the physiological basis of a successful adaptation to freshwater. this study particularly focused on the ability of sea-bass to maintain their hydromineral balance in freshwater and on their ion (re)absorbing abilities through the gills and kidneys. two different responses were recorded after ... | 2005 | 16215214 |
| adaptive bone formation in acellular vertebrae of sea bass (dicentrarchus labrax l.). | mammalian bone is an active tissue in which osteoblasts and osteoclasts balance bone mass. this process of adaptive modelling and remodelling is probably regulated by strain-sensing osteocytes. bone of advanced teleosts is acellular yet, despite the lack of osteocytes, it is capable of an adaptive response to physical stimuli. strenuous exercise is known to induce lordosis. lordosis is a ventrad curvature of the vertebral column, and the affected vertebrae show an increase in bone formation. the ... | 2005 | 16155222 |
| dietary phospholipids are more efficient than neutral lipids for long-chain polyunsaturated fatty acid supply in european sea bass dicentrarchus labrax larval development. | we evaluated the effects of dietary lipid class (phospholipid vs. neutral lipid) and level of n-3 long-chain pufa (lc-pufa) on the growth, digestive enzymatic activity, and histological organization of the intestine and liver in european sea bass larvae. fish were fed from the onset of exogenous feeding at 7 to 37 d post-hatch with five isoproteic and isolipidic compound diets with different levels of epa and dha. diet names indicated the percentage of epa and dha contained in the phospholipids ... | 2005 | 16149740 |
| a microsatellite linkage map of the european sea bass dicentrarchus labrax l. | a genetic linkage map of the european sea bass (dicentrarchus labrax) was constructed from 174 microsatellite markers, including 145 new markers reported in this study. the mapping panel was derived from farmed sea bass from the north adriatic sea and consisted of a single family including both parents and 50 full-sib progeny (biparental diploids). a total of 162 microsatellites were mapped in 25 linkage groups. eleven loci represent type i (coding) markers; 2 loci are located within the peptide ... | 2005 | 15937133 |
| blood chemistry and histological properties of wild and cultured sea bass (dicentrarchus labrax) in the north adriatic sea. | histological and biochemical procedures were employed to study liver histomorphology and plasma levels of aspartate aminotransferases (ast), alanine aminotransferase (alt), triglyceride (trig), cholesterol (chol), glucose (glu) and total protein (tp) in cultured (csb) and wild sea bass (wsb) (dicentrarchus labrax) from the northern part of the adriatic sea. histopathological changes in liver included varying degrees of infiltration and lipid degeneration of hepatocytes in examined cultured fish. ... | 2005 | 16369882 |
| in vivo allograft rejection in a bony fish dicentrarchus labrax (l.): characterisation of effector lymphocytes. | lymphoid cell subpopulations involved in allograft rejection in the teleost dicentrarchus labrax were characterised at the ultrastructural level and quantified by using monoclonal antibodies against t- and b-lymphocytes. t-cells positive for t-cell receptor beta-chain (tcr beta) were detected by reverse transcription/polymerase chain reaction (rt-pcr) and in situ hybridisation by using rna probes for tcr beta. flow cytometry detected a similar percentage of t- and b-lymphocytes (around 17%) in t ... | 2005 | 16047156 |
| effect of periodical water current on the phasing of demand feeding rhythms in sea bass (dicentrarchus labrax l.). | the demand feeding rhythms of sea bass (dicentrarchus labrax l.) maintained at rest or subjected to exercise (increase in swimming activity) as a result of an induced water current are compared. both sea bass kept individually and in group (4 and 8 individuals) shifted their food demands towards the exercise period or to just after the same (the latter especially in individually kept fish). the origin of such a response is discussed in view of innate behaviour, the energy cost of locomotor activ ... | 2005 | 15985274 |
| quantitative aspects of the spatial distribution and morphological characteristics of the sea bass (dicentrarchus labrax l.; teleostei, serranidae) trunk lateral line neuromasts. | the results presented herein report quantitative data relative to the distribution and morphological characteristics of both types of neuromasts encountered on the trunk lateral line of the sea bass (dicentrarchus labrax, l.). these data were obtained from scanning electron micrographs. they indicate that, as expected, each modified scale of the sea bass possessed a single canal neuromast with long axis oriented parallel to the fish's long axis. in contrast to several fish species, two thirds of ... | 2005 | 15761216 |
| morphofunctional ontogeny of the urinary system of the european sea bass dicentrarchus labrax. | european sea bass (dicentrarchus labrax) are euryhaline fish that tolerate wide salinity fluctuations owing to several morphofunctional adaptations. among the osmoregulatory sites (tegument, branchial chambers, digestive tract, urinary system), little is known about the kidney and the urinary bladder. the present study describes the ontogeny of the urinary system (kidney and urinary bladder) and focuses on the progressive expression of the na(+)/k(+)-atpase in the cells of these ion-transporting ... | 2005 | 15616825 |
| bacterial vaccines for fish--an update of the current situation worldwide. | during the last few years, the use of vaccines for disease prevention in aquaculture has expanded both with regard to the number of fish species and number of microbial diseases. according to the responses to a questionnaire received from 41 countries, vaccination is used in the commercial aquaculture of species like atlantic salmon (salmo salar), rainbow trout (oncorhynchus mykiss), sea bass (dicentrarchus labrax), sea bream (sparus aurata), barramundi (lates calcarifer), tilapia (tilapia spp), ... | 2005 | 15962470 |
| sea bass (dicentrarchus labrax) sex hormone binding globulin: molecular and biochemical properties and phylogenetic comparison of its orthologues in multiple fish species. | sex hormone binding globulin (shbg) binds and transports androgens and estrogens in the blood of vertebrate species including fish. we have used oligonucleotide primers corresponding to highly conserved regions of the shbg coding sequences within the zebrafish and fugufish genomes to obtain a 1528 bp cdna encoding shbg from tissue rna extracts from the european sea bass. amino-terminal sequence analysis of recombinant sea bass shbg indicated that its deduced precursor polypeptide includes a 35-r ... | 2005 | 15607525 |
| short- and long-term effects of a dietary yeast beta-glucan (macrogard) and alginic acid (ergosan) preparation on immune response in sea bass (dicentrarchus labrax). | the present study investigated the immunomodulatory activity of ergosan, an algal extract containing alginic acid, and macrogard, a yeast extract containing beta-glucans, on innate and specific immunity in sea bass (dicentrarchus labrax). four cycles of experimental feeding using normal fish feed formulation (control group) supplemented with ergosan (0.5%) or macrogard (0.1%) were performed at 60-day intervals (15 days of treatment+45 days of suspension). serum complement, lysozyme, total protei ... | 2005 | 15561561 |
| epidemiology of cryptosporidium molnari in spanish gilthead sea bream (sparus aurata l.) and european sea bass (dicentrarchus labrax l.) cultures: from hatchery to market size. | a long-term epidemiological study of cryptosporidium molnari in aquacultured european sea bass (esb) and gilthead sea bream (gsb) was performed in different types of facilities on the atlantic, cantabric, and mediterranean coasts. four types of studies were carried out. in study a, fish raised from juveniles to marketable size (ongrowing stage) were periodically sampled in three different types of cultures. studies b and c focused on hatchery and nursery facilities. in study d, occasional sampli ... | 2005 | 15640180 |
| organochlorine compounds in liver and concentrations of vitellogenin and 17beta-estradiol in plasma of sea bass fed with a commercial or with a natural diet. | results from previous experiments directed to determine the effect of different nutritional factors or the effect of xenobiotics on hormonal control of reproduction, lead to the hypothesis that hormonal perturbations repeatedly observed in sea bass (dicentrarchus labrax) broodstock feeding commercial diets could have been caused by the presence of aryl hydrocarbon receptor (ahr) ligands, such as dioxins, furans and polychlorinated biphenyls (pcbs) in the diet. to evaluate this hypothesis, dioxin ... | 2005 | 16213605 |
| effects of 17beta-estradiol, 4-nonylphenol and pcb 126 on the estrogenic activity and phase 1 and 2 biotransformation enzymes in male sea bass (dicentrarchus labrax). | the endocrine system of wildlife is exposed to a wide variety of natural and man-made chemicals which may lead to damage to the reproductive system and other adverse effects, including alteration of drug-metabolizing enzymes. in the present study, the effects of in vivo exposure to a natural (17beta-estradiol: e2) or a xenoestrogen (4-nonylphenol: np) estrogen or an anti-estrogen (3,3',4,4',5-pentachlorobiphenyl: pcb 126) upon vitellogenin (vtg) synthesis and hepatic phase 1 and 2 enzymes have b ... | 2005 | 16219370 |
| kudoa iwatai (myxosporea: multivalvulida) in wild and cultured fish in the red sea: redescription and molecular phylogeny. | gilt-head sea bream, sparus aurata l., the mediterranean's most important mariculture species, has been cultured for the last 30 yr in eilat (israeli red sea). kudoa sp. was the first myxosporean parasite reported from this species. in recent years, an increase in prevalence in both land-based and sea-cage facilities in eilat has been observed. infections with the same kudoa species appeared in cultured european sea bass dicentrarchus labrax (l.) and grey mullet mugil cephalus in the same farms, ... | 2005 | 16419765 |
| confirmation of piscirickettsia salmonis as a pathogen in european sea bass dicentrarchus labrax and phylogenetic comparison with salmonid strains. | european sea bass dicentrarchus labrax from the mediterranean were diagnosed with a severe encephalitis. rickettsia-like organisms (rlos) were associated with brain lesions in routine paraffin sections. these were found to share common antigens with the piscirickettsia salmonis type-strain, lf-89, by indirect fluorescent antibody test (ifat) and by immunohistochemistry (ihc). in addition, we compared the dna sequences of the 16s rdna and 16s-23s internal transcribed spacer region (its) with thos ... | 2005 | 15918473 |
| biological activity of sea bass (dicentrarchus labrax l.) recombinant interleukin-1beta. | biological activities of a putative mature sea bass interleukin-1beta peptide, produced as a recombinant protein (ril-1beta) in escherichia coli, have been investigated. the ril-1beta contains a 6-histidine tag at the n-terminus, and protein purification has been achieved through this tag by affinity chromatography. biological activities have been investigated both at the cellular and gene expression levels. in in vitro assays sea bass ril-1beta induced the proliferation of murine d10.g4.1 cells ... | 2005 | 16059754 |
| hyperparasitism of trichodinid ciliates on monogenean gill flukes of two marine fish. | two unusual cases of hyperparasitism of trichodinid ciliates on monogenean gill flukes are described from southern israel (red sea). the first case occurred in cultured european sea bass dicentrarchus labrax infected by diplectanum aequans, while the second was observed in a feral devil firefish pterois miles infected by haliotrema sp. in both cases, the trichodinids heavily co-infested the host fish gills. the flukes were completely coated by the ciliates, which gave them a cobblestone appearan ... | 2005 | 16060271 |
| side effects in sea bass (dicentrarchus labrax l.) due to intraperitoneal vaccination against vibriosis and pasteurellosis. | sea bass (dicentrarchus labrax l.) were injected intraperitoneally with monovalent (photobacterium damselae subsp. piscicida or vibrio anguillarum) and divalent (ph. damselae subsp. piscicida and v. anguillarum) vaccine formulations, with or without adjuvants (mineral oil, liposome or alginate), to evaluate the short and long-term pathological effects. eight animals from each group were sampled one, two, four and 11 months after intraperitoneal injection. the acute peritoneal response and the pr ... | 2005 | 15722227 |
| monitoring food quality by microfluidic electrophoresis, gas chromatography, and mass spectrometry techniques: effects of aquaculture on the sea bass (dicentrarchus labrax). | monitoring food quality is a critical task for analytical chemistry and an important way to preserve human health. fish is a valuable source of highly digestible proteins and contains large amounts of polyunsaturated fatty acids and fat-soluble vitamins. since the world's wild fish stocks are limited, farmed fish is nowadays proposed as an alternative to consumers. it is now emerging that the fish muscle protein content is assuming great importance from an aquaculture perspective. many data have ... | 2005 | 15828797 |
| vaccination trials of sea bass (dicentrarchus labrax) against pasteurellosis using oral, intraperitoneal and immersion methods. | photobacterium damsela subsp. piscicida (phdp) is the aetiological agent of fish pasteurellosis, causing heavy losses in intensive mariculture plants. the present work compares the protective efficacy of five different vaccine formulation: oral, intraperitoneal, immersion, bivalent immersion (vibrio anguillarum) and immersion associated with immunostimulants. each of these vaccine formulations containing whole cells of phdp formalin inactivated (fkc), was administered to 100 sea bass weighing ap ... | 2005 | 20437375 |
| parasite communities of adriatic cage-reared fish. | from june 2001 to march 2002, 7 semi-offshore facilities in the adriatic sea rearing sea bass dicentrarchus labrax, sea bream sparus aurata, sharpsnout bream diplodus puntazzo and red sea bream pagellus bogaraveo aged 1+, were monitored for the presence of protozoan and metazoan parasites. obtained data sets of abundance and prevalence were used to evaluate the structure and dynamic of infra- and component parasite communities. in all hosts, except the red sea bream, dominant parasites were mono ... | 2005 | 15900691 |
| long-term exposure to continuous light inhibits precocity in european male sea bass (dicentrarchus labrax, l.): hormonal aspects. | the effect of long-term exposure to continuous light on the hormonal aspects of the reproductive axis was investigated in juvenile male sea bass (dicentrarchus labrax, l.) during the first annual cycle. four-month-old fish were exposed to a simulated natural photoperiod (np) and a continuous light (24 h) regime (ll) under natural conditions of temperature (13.3-25.8 degrees c). a dot-blot technique was used to analyse gonadotropin (the common glycoprotein alpha, gpalpha; the follicle stimulating ... | 2005 | 15613274 |
| acth response to lps in the first stages of development of the fish dicentrarchus labrax l. | acth and acth receptor-like molecules were found at the examined stages of development (2, 4, 8, 12, 18, and 24 days post-hatching) in yolk sac, pronephros tubules, interrenal tissue, thymus, liver, spleen, cardinal veins, and skin of the teleost fish dicentrarchus labrax. acth and the related receptor-like molecules show a similar distribution. lps treatment at two different stages (8 and 24 days post-hatching) provoked both a release and an induction of acth-like molecules, suggesting an impor ... | 2005 | 16061067 |
| intra-adrenal interactions in fish: catecholamine stimulated cortisol release in sea bass (dicentrarchus labrax l.). | the effect of the catecholamines, adrenaline and noradrenaline, on sea bass (dicentrarchus labrax) and sea bream (sparus auratus) interrenal cortisol production was studied in vitro using a dynamic superfusion system technique. increasing concentrations of catecholamines (10(-6), 10(-8) and 10(-10) m) stimulated cortisol production in a dose-dependent manner, in sea bass only. the increase in cortisol production stimulated by adrenaline (10(-6) m) and noradrenaline (10(-6) m) was inhibited by so ... | 2006 | 16443381 |
| effects of gutting and ungutting on microbiological, chemical, and sensory properties of aquacultured sea bream (sparus aurata) and sea bass (dicentrarchus labrax) stored in ice. | the effect of gutting and ungutting on microbiological, chemical, and sensory properties of aqua-cultured sea bream (sparus aurata) and sea bass (dicentrarchus labrax) stored in ice were studied. the total viable mesophilic and psychrophilic bacterial counts increased throughout the storage period of gutted and ungutted sea bream and sea bass. the mesophilic counts reached 8.19 log cfu/g for ungutted sea bream and 7.93 log cfu/g for ungutted sea bass after 14 days of storage. the mesophilic coun ... | 2006 | 16954060 |
| the use of a test battery in marine ecotoxicology: the acute toxicity of sodium dodecyl sulfate. | for a toxicity assessment of substances entering the marine environment, it is preferable to carry out ecotoxicological tests on a base-set of taxa utilizing target species belonging to different trophic levels. in this study a battery composed of vibrio fischeri (bacteria), dunaliella tertiolecta (algae), tigriopus fulvus (crustacea), paracentrotus lividus (echinodermata), and dicentrarchus labrax (pisces) was used for acute toxicity testing of sodium dodecyl sulfate (sds). sds is an anionic su ... | 2006 | 16841322 |
| parasitic diseases of marine fish: epidemiological and sanitary considerations. | over recent decades, parasitic diseases have been increasingly considered a sanitary and economic threat to mediterranean aquaculture. in order to monitor the distribution of parasites in cultured marine fish from italy and study their pathogenic effects on the host, a three-year survey based on parasitological and histopathological exams was carried out on 2141 subjects from eleven fish species and coming from different farming systems (extensive, intensive inland farms, inshore floating cages, ... | 2006 | 16881387 |
| bioaccumulation of some heavy metals in different tissues of dicentrarchus labrax l, 1758, sparus aurata l, 1758 and mugil cephalus l, 1758 from the camlik lagoon of the eastern coast of mediterranean (turkey). | in this study, bioaccumulation of the heavy metals (fe, zn, cd) in the liver, gill, gonad and muscle tissues of dicentrarchus labrax, mugil cephalus and sparus aurata were investigated seasonally. camlik is the important fisheries lagoon located in the iskenderun bay, mediterranean coast of turkey. due to heavy industrial and agricultural activities in the region, the bay has the polluted coastal waters of turkey. therefore, the levels of cadmium, zinc, iron were determined in the muscle in each ... | 2006 | 16897534 |
| induction of a protective immune response against viral nervous necrosis in the european sea bass dicentrarchus labrax by using betanodavirus virus-like particles. | betanodaviruses are causative agents of viral nervous necrosis (vnn), a devastating disease of cultured marine fish worldwide. virus particles contain a single type of coat protein that spontaneously assembles into virus-like particles (vlps) when expressed in a baculovirus expression system. in the present study, the immunogenicity of betanodavirus vlps and the protection they confer against vnn in the european sea bass dicentrarchus labrax were investigated. enzyme-linked immunosorbent assay a ... | 2006 | 17005697 |
| partial replacement of dietary fish oil with blends of vegetable oils (rapeseed, linseed and palm oils) in diets for european sea bass (dicentrarchus labrax l.) over a long term growth study: effects on muscle and liver fatty acid composition and effectiveness of a fish oil finishing diet. | triplicate groups of european sea bass (dicentrarchus labrax l.), of initial mass 5 g, were fed one of three practical type diets for 64 weeks. the three diets differed only in the added oil and were 100% fish oil (fo; diet a), 40% fo/60% vegetable oil blend (vo; diet b) where the vo blend was rapeseed oil, linseed oil and palm oil in the ratio 10/35/15 by weight and 40% fo/60% vo blend (diet c) where the ratio was 24/24/12 by weight. after final sample collection the remaining fish were switche ... | 2006 | 17055762 |
| immersion booster vaccination effect on sea bass (dicentrarchus labrax l.) juveniles. | in each challenge 30 sea bass juveniles (mean weight 3.3 +/- 0.2 g sd) were used. during the whole experiment (water t: 18 +/- 1 degrees c) the fish were held in four 50l seawater independent recirculation systems (one fish group per 50l system). the protection to the pathogen vibrio anguillarum was tested on booster vaccinated sea bass (dicentrarchus labrax l.) juveniles. the vaccination was performed by immersion for 60 s in a commercial anti-v. anguillarum vaccine suspension. booster vaccinat ... | 2006 | 16422769 |
| temperature-dependency of betanodavirus infection in ssn-1 cell line. | this study examined the in vitro effects of temperature on betanodavirus infection in the ssn-1 cell line. a betanodavirus isolated from moribund sea bass fry dicentrarchus labrax farmed in the adriatic sea and characterised as a rgnnv (redspotted grouper nervous necrosis virus) genotype was used. virus-infected ssn-1 cells were incubated at temperatures between 10 and 30 degrees c and observed for cytopathic effects daily for 15 d. cell-free and cell-associated viral growth were evaluated by 50 ... | 2006 | 16610592 |
| dual function of fish hepcidin: response to experimental iron overload and bacterial infection in sea bass (dicentrarchus labrax). | the role of hepcidin in iron metabolism regulation and bacterial infection has been the focus of recent attention. however, in spite of the growing number of hepcidin genes known from different organisms, little is known about its putative dual function in fish. the aim of this study was to characterize the sea bass hepcidin gene and to study its role in iron metabolism and infection. the novel sea bass hepcidin gene was found to be organized into two introns and three exons with several copies ... | 2006 | 16616368 |
| evolution of cytokine responses: il-1beta directly affects intracellular ca2+ concentration of teleost fish leukocytes through a receptor-mediated mechanism. | in this work we studied the biological activities of recombinant il-1beta from the teleosts sea bass (dicentrarchus labrax) and rainbow trout (oncorhynchus mykiss) by investigating the effects induced on intracellular ca2+ concentrations ([ca2+]i) of spleen leucocytes. splenocytes were loaded with the ca2+-permeant fura-2am, and then stimulated with ril-1beta. the emitted fluorescence was read for 5 min at 1 min intervals on a dual excitation fluorescence fluorimeter. results showed that ril-1be ... | 2006 | 16713284 |
| efficacy of a listonella anguillarum (syn. vibrio anguillarum) vaccine for juvenile sea bass dicentrarchus labrax. | the efficacy of a commercial bivalent listonella anguillarum (serotype 01 and 02) vaccine (microvib, microtek international) was tested on prime- and booster-immersion vaccinated sea bass dicentrarchus labrax juveniles. we carried out 2 challenge tests on the prime-vaccinated fish, 50 and 90 d after initial vaccination. a second group of fish received a booster vaccination 60 d after the prime vaccination, and were tested with a single challenge 30 d later. relative percent survival (rps) was 92 ... | 2006 | 16921997 |
| environmental impact of the marine aquaculture in güllük bay, turkey. | the effects of marine aquaculture on the environment were evaluated by studying the water quality of güllük bay (turkey). marine aquaculture, both extensive and intensive, is one of the most important activities carried out in this area. the intensive culture of fish (dicentrarchus labrax) is the most important polluting element in güllük bay. records of long term (seasonally) monitoring of the ambient water dissolved oxygen concentration, the inorganic nutrients (ammonia, nitrate, nitrite, and ... | 2006 | 17082906 |
| virulence and enterobacterial repetitive intergenic consensus pcr of vibrio alginolyticus strains isolated from tunisian cultured gilthead sea bream and sea bass outbreaks. | vibrio alginolyticus was isolated from the internal organs of diseased gilthead sea bream (sparus aurata) and sea bass (dicentrarchus labrax) cultured in two fish farms located on the tunisian mediterranean coast, from 2003 to 2005. after phenotypic characterisation, a selection of 34 isolates from gilthead sea bream and sea bass were molecularly typed by repetitive intergenic consensus pcr (eric-pcr) showing a high polymorphism among the isolated strains (19 genotypes). most of the isolates wer ... | 2006 | 16870360 |
| the cd8alpha from sea bass (dicentrarchus labrax l.): cloning, expression and 3d modelling. | in this paper we describe the cloning, expression and structural study by modelling techniques of the cd8alpha from sea bass (dicentrarchus labrax l.). the sea bass cd8alpha cdna is comprised of 1490 bp and is translated in one reading frame to give a protein of 217 amino acids, with a predicted 26 amino acids signal peptide, a 88 bp 5'-utr and a 748 bp 3'-utr. a multiple alignment of cd8alpha from sea bass with other known cd8alpha sequences shows the conservation of most amino acid residues in ... | 2006 | 16230027 |
| molecular cloning and characterization of sea bass (dicentrarchus labrax l.) cd8alpha. | in this work, the gene and cdna of the sea bass cd8alpha have been isolated and characterized. the coding sequence has an orf of 666 bp. it retains the ig motif that interacts with mhc and the two cysteines responsible for an intra-chain disulfide bridge. the hinge region contains the two essential cysteines involved in dimerization. the transmembrane region is well conserved in all analysed sequences. similar to other teleosts, the cytoplasmic region lacks the consensus p56(lck) motif common in ... | 2006 | 16414122 |
| husbandry stress during early life stages affects the stress response and health status of juvenile sea bass, dicentrarchus labrax. | in aquaculture management it is important to establish objective criteria to assess health and welfare of the fish. here we show that european sea bass (dicentrarchus labrax) confronted with husbandry-associated stress (tank cleaning, i.e. scrubbing, and water temperature variation) during early life stages show poorer survival and disease resistance as juveniles. we evaluated several parameters for stress (plasma cortisol, glucose and lactate, hydromineral status), growth performance, the immun ... | 2006 | 15961320 |
| construction and characterization of a bac library for the european sea bass dicentrarchus labrax. | 2006 | 16978191 | |
| est-based identification of genes expressed in the liver of adult seabass (dicentrarchus labrax, l.). | the scarcity of the genomic resources for some fish species, in spite of their commercial interest, could retard the positive effects that modern biotechnology can offer to aquaculture industry. then an effort should be made to reduce, as far as it concerns genomic resources, the gap that separates farming species from "model organisms". in this paper, we present an est project in which we performed single pass sequencing on 1229 randomly selected clones from a sea bass cdna library. the sequenc ... | 2006 | 16616996 |
| digestive tract ontogeny of dicentrarchus labrax: implication in osmoregulation. | the ontogeny of the digestive tract (dt) and of na(+)/k(+)-atpase localization was investigated during the early postembryonic development (from yolk sac larva to juvenile) of the euryhaline teleost dicentrarchus labrax reared at two salinities: seawater and diluted seawater. histology, electron microscopy and immunocytochemistry were used to determine the presence and differentiation of ion transporting cells. at hatching, the dt is an undifferentiated straight tube over the yolk sac. at the mo ... | 2006 | 16573732 |
| effects of chemical lipid extraction and arithmetic lipid correction on stable isotope ratios of fish tissues. | for accurate interpretation of fish trophodynamics from carbon stable isotope data it is necessary to extract tissue lipids. this is because lipid content varies within and among tissues in both space and time, and because lipids are 13c-depleted relative to proteins. however, lipid extraction may affect delta15n, thus requiring costly and time-consuming separation of delta13c and delta15n analyses. these problems have prompted the development of arithmetic correction techniques for delta13c, bu ... | 2006 | 16429479 |
| effects of oleic acid on the high threshold barium current in seabass dicentrarchus labrax ventricular myocytes. | the present study employed a patch clamp technique in isolated seabass ventricular myocytes to investigate the hypothesis that oleic acid (oa), a mono-unsaturated fatty acid, can exert direct effects upon whole-cell barium currents. acute application of free oa caused a dose-dependent depression of the whole-cell barium current that was evoked by a voltage step to 0 mv from a holding potential of -80 mv. the derived 50% inhibitory concentration (ic50) was 12.49+/-0.27 micromol l(-1). at a concen ... | 2006 | 17023597 |
| effect of temperature on maximum swimming speed and cost of transport in juvenile european sea bass (dicentrarchus labrax). | this study is an attempt to gain an integrated understanding of the interactions between temperature, locomotion activity and metabolism in the european sea bass (dicentrarchus labrax). to our knowledge this study is among the few that have investigated the influence of the seasonal changes in water temperature on swimming performance in fish. using a brett-type swim-tunnel respirometer the relationship between oxygen consumption and swimming speed was determined in fish acclimatised to 7, 11, 1 ... | 2006 | 16916977 |
| effects of using slurry ice during transportation on the microbiological, chemical, and sensory assessments of aquacultured sea bass (dicentrarchus labrax) stored at 4 degrees c. | slurry ice, a biphasic system consisting of small spherical ice crystals surrounded by seawater at subzero temperature, was evaluated as a new chilled storage method for whole sea bass (dicentrarchus labrax) a sparidae fish species of remarkable commercial interests. in this study two different group of chilling methods were used during transportation; in slurry ice packaged (group a), and flake ice packaged (group b). the effect of this advanced system during transportation on quality losses an ... | 2006 | 16864138 |
| effect of normal and waxy maize starch on growth, food utilization and hepatic glucose metabolism in european sea bass (dicentrarchus labrax) juveniles. | we determined the effect of dietary starch on growth performance and feed utilization in european sea bass juveniles. data on the dietary regulation of key hepatic enzymes of the glycolytic, gluconeogenic, lipogenic and amino acid metabolic pathways (hexokinase, hk; glucokinase, gk; pyruvate kinase, pk; fructose-1,6-bisphosphatase, fbpase; glucose-6-phosphatase, g6pase; glucose-6-phosphate dehydrogenase, g6pd; alanine aminotransferase, alat; aspartate aminotransferase, asat and glutamate dehydro ... | 2006 | 16343962 |
| intake of high levels of vitamin a and polyunsaturated fatty acids during different developmental periods modifies the expression of morphogenesis genes in european sea bass (dicentrarchus labrax). | the effect of the feeding period on larval development was investigated in european sea bass larvae by considering the expression level of some genes involved in morphogenesis. larvae were fed a control diet except during three different periods (period a: from 8 to 13 d post-hatching (dph); period b: from 13 to 18 dph; period c: from 18 to 23 dph) with two compound diets containing high levels of vitamin a or pufa. european sea bass morphogenesis was affected by these two dietary nutrients duri ... | 2006 | 16571146 |
| the effects of biological fouling control at coastal and estuarine power stations. | the use of low levels of oxidants as antifouling agents in coastal and estuarine power station cooling water circuits can give rise to concerns over the likely production of chlorination byproducts (cbps) including organohalogens, of largely cryptic identity, loading and impact. in order to review the current understanding of the degree of impact involved this paper describes a recent collaborative programme of work on cbps within the wider context of a number of allied studies on power station ... | 2006 | 16497340 |
| effects of experimental terbuthylazine exposure on the cells of dicentrarchus labrax (l.). | the effects of acute exposure to the herbicide terbuthylazine (3.55, 5.01 and 7.08 mg l(-1)) on the cells of farmed european sea bass, dicentrarchus labrax l., were investigated by means of light and electron microscopy. in gills of treated fish, the number of chloride cells (ccs) and rodlet cells (rcs) increased significantly within 24 h and 48 h, respectively; the intestine showed the largest increase in rcs linked to treatment and exposure time. in kidney, 24 h exposure induced a significant ... | 2006 | 16487568 |
| biotransformation, stress and genotoxic effects of 17beta-estradiol in juvenile sea bass (dicentrarchus labrax l.). | the effects of 17beta-estradiol (e(2)) on fish became a matter of concern, since significant levels of this hormone were detected in the aquatic environment released mainly by domestic sewage treatment plants. in this perspective, the current study was focused on e(2) effects upon biotransformation, stress and genotoxic responses of juvenile dicentrarchus labrax l. (sea bass). fish were exposed to e(2) during 10 days in two different ways: water diluted (200 ng/l or 2,000 ng/l) and i.p. injected ... | 2006 | 16483653 |
| effect of photoperiod on the fish innate immune system: a link between fish pineal gland and the immune system. | the pineal gland via its secretory product, melatonin, influences the light-dark rhythm in most vertebrates including fish. apart from the information concerning this circadian rhythm, the interrelation of the melatonin with other physiological processes has not been considered in fish. thus, we evaluated the changes in the humoral innate immune system of seabream (sparus aurata l.) and sea bass (dicentrarchus labrax l.) specimens exposed to a constant light-dark photoperiod (12 hr l:12 hr d). s ... | 2006 | 16948787 |
| associations between tissue fatty acid composition and physiological traits of performance and metabolism in the seabass (dicentrarchus labrax). | seabass were fed for 4 months with diets where the lipid was provided as either canola oil (co), palm oil (po) or fish oil (fo), to generate diversity in their tissue fatty acid (fa) composition and investigate how this influenced major traits of exercise performance, cardiac performance and respiratory metabolism. in particular, based upon previous observations, we investigated the hypothesis that enriching the fish tissues with oleic and linoleic acids (oa, 18:1n-9 and la, 18:2n-6, respectivel ... | 2006 | 16916978 |