Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| inhibitory effects of an aromatic retinoid (ro 10-9359) on viral tumorigenesis. | prophylactic treatment of hamsters and chickens with an aromatic retinoid (ro 10-9359) inhibited rous sarcoma virus (rsv) tumorigenesis. retinoid administration to chickens with rsv-induced tumors resulted in tumor regression and/or confinement to the primary site. the retinoid also exerted a therapeutic effect on shope virus papilloma development in the skin of rabbits. | 1980 | 6265402 |
| antisera from mice and rats incubated with syngeneic rous sarcoma cells. | cdf1, (balb/c x dba/2)f1, mice were repeatedly inoculated with transplantable mouse rous sarcoma (sr-cdf1) cells which were half-killed by 60co-irradiation beforehand to attain prolonged immunization. the sera obtained from these mice contained antibodies against the src and gag gene products of rous sarcoma virus (rsv). on the other hand, fischer rats inoculated with syngeneic rat rous cells transformed by bryan high titer strain of rsv (bh(-)-3y1) produced antibodies against the gag gene produ ... | 1980 | 6259386 |
| rsv-transformed mammalian cells: an analysis of rnas specific for exogenous and endogenous viruses. | a marked decrease in expression of rsv-specific sequences was observed in cryptovirogenic rsv-transformed rvp3 murine cells during passaging. simultaneously an increase in expression of sequences specific for c-type murine viruses was observed both in these cells and other rsv-transformed murine cells. no activation of c-type viral genome was detected in spontaneous murine lung tumors and in methylcholanthrene-induced murine tumors. four types of virus specific rnas were found in xc cells: the 3 ... | 1980 | 6257202 |
| identification of a functional promoter in the long terminal repeat of rous sarcoma virus. | specific initiation of transcription of rous sarcoma virus by rna polymerase ii was obtained in a cell-free system using cloned rsv dna as template. the site of initiation is located in the common "c" region of the long terminal repeat (ltr), 23 bp downstream from the promoter-like sequence tatttaag. this finding indicates that the basic information necessary for rsv transcription lies within the viral genome. | 1980 | 6257399 |
| ornithine decarboxylase induction during b1 progression of normal and rous sarcoma virus-transformed cells. | ornithine decarboxylase (odc) induction during g1 phase of the cell cycle was compared in rat-1 fibroblasts and in rat-1 fibroblasts transformed by the b77 wild-type rous sarcoma virus (rsv) and by the thermosensitive mutant la24/rsv. in rat-1 cells, maximal enzyme activity detectable at mid g1 declined to basal levels by g1-s. the odc increase was inhibited by actinomycin d and cycloheximide and was dependent on the addition of serum growth factors. rat-1 (b77 wild-type rsv) cells expressed a g ... | 1980 | 6258784 |
| increase in s-adenosyl-l-methionine decarboxylase activity during the transformation of chick embroy fibroblasts by rous sarcoma virus. | the increase in s-adenosyl-l-methionine decarboxylase activity in chick embryo fibroblasts after infection with rous sarcoma virus has been studied. it has been shown that enzyme levels in transformed cells were two or three times higher than those of the non-infected controls. the activity of this enzyme was not elevated in chick embryo fibroblasts infected with a temperature sensitive mutant of rous sarcoma virus (rsv-t5) at 42 degrees c, the non-permissive temperature. when the temperature of ... | 1980 | 6263805 |
| prevention of infections with respiratory syncytial virus: the hopes and hurdles ahead. | control of infections due to respiratory syncytial virus (rsv) by immunization poses special problems. first, the peak period of serious illness due to rsv is during the first few months of life, and thus a vaccine would have to be administered during the neonatal period. second, we understand little of the pathogenesis of and immunity to rsv disease in newborns, and an immune reaction may even play a role in the development of the lower respiratory tract disease seen in infancy. third, immunity ... | 1980 | 6997967 |
| [critical considerations on the laboratory research in infections from respiratory syncytial virus (rsv) (author's transl)]]. | 1980 | 7008714 | |
| respiratory syncytial virus serology by a simplified enzyme-linked immunosorbent assay. | a simplified enzyme-linked immunosorbent assay (elisa) which utilized commercially available reagents was developed for respiratory syncytial virus (rsv)-specific immunoglobulin g. an analysis of the inherent variation of the assay allowed the setting of strict criteria for determining a significant change in rsv antibodies. the elisa was more sensitive than the standard complement fixation or microneutralization tests in a carefully studied group of 32 rsv-infected adults. the elisa correlated ... | 1980 | 7012179 |
| the possible role of respiratory syncytial virus and pasteurella spp in calf respiratory disease. | in a natural outbreak of respiratory disease during 1976, 31 of 43 calves showed moderately severe clinical signs which included pyrexia (maximum 42 degrees c) and tachypnoea. during the outbreak infection by respiratory syncytial virus (rsv) was demonstrated by isolation from nasopharyngeal swabs or by serology. pasteurella haemolytica or p multocida were isolated from the blood of four and five calves respectively. in the month before disease 65 per cent of the calves showed significant antibo ... | 1980 | 7210428 |
| respiratory syncytial virus infections in oslo 1972--1978. i. virological and epidemiological studies. | respiratory syncytial virus (rsv) infection was diagnosed in 464 children admitted to hospital with acute respiratory disease from autumn 1972 to spring 1978. the virological surveillance was approximately 70%. as a rule, rsv infections occurred in distinct outbreaks in late autumn and winter together with a marked increase in the number of children admitted to hospital with acute lower respiratory tract disease. the incidence in children less than 1 year of age was about 10/1000 children/year. ... | 1980 | 7211356 |
| respiratory syncytial virus infections in oslo 1972--1978. ii. clinical and laboratory studies. | patient records from 422 children admitted to hospital during 6 winter outbreaks of rsv infection were examined retrospectively. bronchiolitis (255 patients) and pneumonia (98 patients) were the most common diagnoses. otitis media (76 patients) occurred as a complication to these conditions. children at risk for contracting severe illness caused by rsv were infants below the age of one year, more often boys than girls. of the children older than two years, 32% had already been diagnosed as asthm ... | 1980 | 7211357 |
| [prospects of respiratory syncytial virus vaccines]. | the latest vaccines for respiratory syncytial virus (rsv) are reviewed. a ts-1 variant is being studied in the attempt to complete the attenuation of the vaccine strain. further investigations of live rsv vaccine administered parenterally indicate that a target population over 6 months of age could benefit soon of this preparation. | 1980 | 7247482 |
| possible transmission by fomites of respiratory syncytial virus. | to test whether nosocomial spread of respiratory syncytial virus (rsv) could occur through contact with environmental surfaces contaminated by rsv-infected nasal secretions, survival in the environment of rsv isolated from media, pooled adult secretions, and secretions from hospitalized infants was examined. rsv in freshly obtained infant secretions was recovered from countertops for up to 6 hr, from rubber gloves for up to 1 1/2 hr, from cloth gowns and paper tissue for 30--45 min, and from ski ... | 1980 | 7365274 |
| the antibody response to primary and secondary infection with respiratory syncytial virus: kinetics of class-specific responses. | serum antibody responses to primary and secondary infections with respiratory syncytial virus were determined in 67 hospitalized infants. responses in igg, igm, and iga immunoglobulin fractions were assayed using an indirect immunofluorescent technique. infection was confirmed by identification of rsv antigen in nasopharyngeal secretions using indirect immunofluorescence, and by recovery of the virus in tissue culture. igm specific antibody response was commonly observed within a few days of ons ... | 1980 | 7365579 |
| morphogenesis and structure of caprine respiratory syncytial virus. | cell cultures inoculated with caprine respiratory syncytial virus (rsv) were studied with light, fluorescent, and electron microscopy to determine the morphogenesis and structure of the virus. small syncytia were seen after 36 hours in culture. after 48 hours in culture, syncytia were large and numerous and pleomorphic cytoplasmic inclusions were seen. these inclusions were more pronounced and numerous later in the infection cycle. indirect immunofluorescence revealed a diffuse to granular cytop ... | 1980 | 7416970 |
| the appearance of cell-bound ige in respiratory-tract epithelium after respiratory-syncytial-virus infection. | we studied the appearance of ige in the respiratory tract in 42 infants and young children with various forms of respiratory illness after infection by respiratory syncytial virus (rsv). ige was bound to exfoliated nasopharyngeal epithelial cells in most patients with rsv infection during the acute phase of infection, regardless of the form of illness. however, the continued presence of cell-bound ige was more common in patients with rsv-induced bronchiolitis or asthma than in patients with mild ... | 1980 | 7421946 |
| [association between viral infection and pleuropericarditis: study of a case list of pleurisy and pericarditis]. | the authors report a study on a group of 47 patients affected by serositis (pleuritis, pericarditis, pleuro-pericarditis) of suspected viral origin. two serum samples were taken from each patient at different times and were tested with many viral antigens by the complement fixation technique. the discussion is carried out on the lines followed by the majority of people in giving importance to their viral etiology and the viruses involved as agents of serositis are reported. in the present study ... | 1980 | 7459017 |
| effect of respiratory syncytial virus and virus-antibody complexes on the oxidative metabolism of human neutrophils. | the effect of respiratory syncytial virus (rsv) or mixtures of rsv and its specific antibody on the oxidative metabolic activity of human polymorphonuclear leukocytes was studied by the technique of luminol-dependent chemiluminescence. peripheral blood neutrophils obtained from normal healthy donors were used. rsv alone failed to induce any chemiluminescent response by the neutrophils. however, mixtures of rsv and rsv antibody-positive serum regularly elicited significant neutrophil chemilumines ... | 1981 | 7251140 |
| bronchomammary axis in the immune response to respiratory syncytial virus. | the products of lactation from 26 nursing mothers were sequentially examined over several months for the presence or appearance of antibodies directed against respiratory syncytial virus. antiviral igm and igg were rarely identified in either colostrum or milk. rsv-specific iga was found in 75% (18/24) of specimens of colostrum; 40% (6/15) and 59% (4/7) of milk samples obtained at three and six months still contained specific iga antibody. the latter increase was felt to represent boosting of ex ... | 1981 | 7252673 |
| activation of complement by cells infected with respiratory syncytial virus. | the ability of respiratory syncytial virus (rsv)-infected he(p)-2 cells in culture to activate complement was investigated. after incubation of cells with various complement sources and buffer, binding of c3b to surfaces of infected cells was demonstrated by immunofluorescence with a double-staining technique. nonsyncytial and syncytial (i.e., fused, multinucleated) cells were separately enumerated. also, lysis of rsv-infected cells was assessed by lactic dehydrogenase release. in this system on ... | 1981 | 7263071 |
| secretory antibody response to respiratory syncytial virus infection. | the secretory antibody response to primary and secondary infection with respiratory syncytial virus (rsv) in igg, igm and 11s iga immunoglobulin classes was determined using an indirect immunofluorescence antibody technique. nasopharyngeal secretions were collected after naturally acquired primary or secondary infection with rsv. immunoglobulin a responses to rsv were significantly greater in infants older than 6 months of age at the time of infection than in younger infants. reinfection with rs ... | 1981 | 7294005 |
| correlation of proliferation of lung epithelium with intramuscular sensitization and complement-fixing antibody to respiratory syncytial virus in the golden hamster. | intramuscular sensitization of hamsters with several forms of respiratory syncytial virus (rsv) caused proliferation of lung epithelium. in contrast, intranasal injection of live virus rarely resulted in this phenomenon. a correlation existed between proliferation of lung epithelium and presence of complement-fixing antibody, but not between lung disease and delayed skin reactions. complement-fixing antibody to rsv was found to be independent of the influence of the thymus. | 1981 | 7337963 |
| nosocomial respiratory syncytial viral infections. should gowns and masks be used? | the efficacy of infection control procedures utilizing gowns and masks in the control of nosocomial respiratory syncytial virus (rsv) infections was evaluated by comparing the rate of nosocomial rsv infections in infants and ward personnel during two sequential periods when gowns and masks were used (period 1) and not used (period 2). all patients (162) and staff (36) on our infants' ward were examined for signs of respiratory infection and had nasal washes obtained for viral isolation every two ... | 1981 | 7234784 |
| [immunological studies of the relation between respiratory syncytial virus (rsv) and bronchiolitis]. | 1981 | 7242476 | |
| legionnaires' disease combined with erythema multiforme in a 3-year-old boy. | a case of legionnaires' disease (ld) is described in a 3-year-old boy. he had fulminant disease with typical signs like bilateral pneumonia, gastrointestinal symptoms, and somnolence indicating involvement of the central nervous system. there was no premorbidity. an outstanding development was erythema multiforme, which has never previously been described in ld. the basic disease was caused by legionella pneumophila. this is evidenced by specific serum igm at admission and a subsequent significa ... | 1981 | 7246139 |
| maternal antibody and respiratory syncytial virus infection in infancy. | one hundred newborn infants were studied prospectively for 1 year for evidence of infection with respiratory syncytial virus (rsv). the indirect membrane fluorescence technique was used to determine specific antibody in sera. infection was shown in 29 cases. in 31 infants exposed to an rsv epidemic season, there was no evidence of infection. maternal antenatal sera were also tested, and wide range of igg antibody to rsv was found. mean titre of maternal igg antibody to rsv was significantly high ... | 1981 | 7038043 |
| comparison of fluorescent-antibody, neutralizing-antibody, and complement-enhanced neutralizing-antibody assays for detection of serum antibody to respiratory syncytial virus. | a comparison of three assays for the detection of serum antibody to respiratory syncytial virus (rsv) was carried out on 47 serum samples obtained sequentially from infants and young children with rsv infection. neutralizing-antibody (na) activity was determined by a semimicromethod of plaque reduction. complement-enhanced na activity was determined by the addition of guinea pig complement to na assays. rsv antibody responses in immunoglobulin g, immunoglobulin m, and immunoglobulin a classes we ... | 1981 | 7016915 |
| clinical and laboratory diagnosis of respiratory syncytial virus infection. | infection with rsv is one of the most common respiratory viral infections in childhood, particularly in infants under 1 year of age. the disease is characterized by epidemic proportions of infection which occur regularly in all parts of the world in the winter months. the spectrum of the clinical disease is quite variable, and the infection may present as bronchiolitis, pneumonia, croup, or exacerbation of pre-existing reactive airway disease. recent evidence has suggested that rsv infection may ... | 1981 | 7018832 |
| diagnosis of respiratory syncytial virus infection in the bovine respiratory tract by immunofluorescence. | direct staining of nasopharyngeal smears with hyperimmune bovine serum raised against respiratory syncytial virus (rsv) and conjugated with fluorescein isothyocyanate was compared with virus isolation from 14 experimentally infected animals. twenty-four out of 107 specimens examined were positive by the fluorescent antibody (fa) method and 21 out of 107 by virus isolation. most of the fa positives (81 per cent) were detected after the ninth day of infection whereas 80 per cent of virus isolation ... | 1981 | 7027594 |
| serum migration-inhibitory activity in infants with respiratory syncytial virus bronchiolitis. | using an unidirectional leucocyte migration-inhibition (lmi) assay, we investigated the migration-inhibitory activity (lif) in the sera of 25 infants who developed bronchiolitis following respiratory syncytial virus (rsv) infection. blood samples for serum-lif activity detection were obtained from patients the day after admission and two weeks later. the lmi assay revealed serum-lif activity in 17 (68%) blood samples taken during the acute phase of the disease. in contrast, no inhibition was fou ... | 1981 | 7030363 |
| production of antiserum to respiratory syncytial virus polypeptides: application in enzyme-linked immunosorbent assay. | by use of crossed immunoelectrophoresis techniques, respiratory syncytial (rs) virus-specific precipitates were produced between rs virus cellular antigen [solubilized in tris(hydroxymethyl)aminomethane-glycine buffer, ph 9] and antiserum raised in rabbits against semipurified rs virus. when these precipitates were employed as antigens for further immunizations in rabbits, antibodies (anti-rsv-precip.i) were produced which reacted with only one rs virus antigen when tested by the crossed immunoe ... | 1981 | 7031083 |
| atopy does not predispose to rsv bronchiolitis or postbronchiolitic wheezing. | twenty-six 8-year-old children who had had respiratory syncytial virus (rsv) bronchiolitis in infancy and their paired controls underwent skin and blood tests to assess the role of immunodeficiency and atopy in the pathogenesis of rsv bronchiolitis and the wheezing that may follow it. there was no difference between patients and controls in prevalence of atopy; positive results of prick tests to common antigens; eosinophil counts; yeast opsonisation defect; c2 deficiency; igg, iga, igm, and ige ... | 1981 | 6788213 |
| respiratory infections in cystic fibrosis patients caused by virus, chlamydia and mycoplasma--possible synergism with pseudomonas aeruginosa. | 116 cystic fibrosis patients were observed, by monthly examinations over an eight-month period, to investigate the importance of non-bacterial respiratory infections (nbi) in exacerbations of the respiratory disease. sputum was examined for bacteria, and serum investigated for antibody response against virus, mycoplasma and chlamydia and for antibodies against pseudomonas aeruginosa. during this period each patient had, on an average, 2.9 exacerbations of which 76% were associated with bacteria, ... | 1981 | 6798822 |
| synthetic peptide fragment of src gene product inhibits the src protein kinase and crossreacts immunologically with avian onc kinases and cellular phosphoproteins. | all the known avian sarcoma viruses have associated protein kinase activities that phosphorylate tyrosine residues of their target proteins. a decapeptide fragment of pp60src of rous sarcoma virus (rsv), residues 415-424, and an analog of that sequence have been chemically synthesized by solid-phase methods. the two decapeptides were not phosphorylated by pp60src of rsv, p90 of y73 avian sarcoma virus, or p140 of fujinami sarcoma virus. however, both peptides were able to inhibit competitively t ... | 1981 | 6801651 |
| a third class of avian sarcoma viruses, defined by related transformation-specific proteins of yamaguchi 73 and esh sarcoma viruses. | the gag-linked transformation-specific protein (polyprotein) p80 of esh avian sarcoma virus (esv) has been compared by tryptic peptide mapping with the homologous protein p90 of yamaguchi 73 avian sarcoma virus (y73). p80 of esv and p90 of y73 were found to share all four of their major nonstructural, transformation-specific, methionine-containing peptides and to have at least seven cysteine-containing transformation-specific peptides in common. two nonstructural cysteine-containing peptides uni ... | 1981 | 6264485 |
| increased growth of rsv-induced tumors in chickens partially tolerant to mhc alloantigens. | chickens with b2b2 mhc genotypes were made partically tolerant to b5 mhc cell-surface antigens and the fate of their rous-sarcoma-virus (rsv)-induced tumors was determined. b2b2 chickens partially tolerant to viable or lysed white blood cells (wbc) or viable red blood cells (rbc) from b5b5 chickens had a significantly higher incidence of tumor progression than untreated, pbs-treated, or b2b2 chickens inoculated with wbc from other b2b2 chickens. the criteria for tolerance were absence of antibod ... | 1981 | 6259056 |
| in vitro phosphorylation of the 36k protein in extract from rous sarcoma virus-transformed chicken fibroblasts. | when cell-free extracts of chickens embryo fibroblasts transformed by rous sarcoma virus (rsv) were incubated with [gamma-32p]atp, a protein having a mr of 36,000 was phosphorylated. two-dimensional electrophoresis of a mixture of phosphorylated proteins formed in vitro and in vivo showed that they are indistinguishable. the in vitro phosphorylation of the mr = 36,000 protein was completely inhibited by serum isolated from rabbit bearing tumor formed by rsv. in addition, phosphorylation of the 3 ... | 1981 | 6259148 |
| external cell surface protein phosphorylation in normal and rous sarcoma virus transformed chick embryo fibroblasts. | normal and rous sarcoma virus (rsv)-transformed chick embryo fibroblasts growing on plastic dishes were incubated with atp (gamma 32p) in situ to detect external cell surface protein kinase activity. under the conditions employed, 32p was incorporated exclusively into proteins, specifically those at the external cell surface, as radioactivity was removed by trypsin treatment of labeled whole cells. in addition, exogenous histones were phosphorylated when added to the reaction mixture. cyclic nuc ... | 1981 | 6259183 |
| detection of respiratory syncytial, parainfluenza type 2, and adenovirus antigens by radioimmunoassay and enzyme immunoassay on nasopharyngeal specimens from children with acute respiratory disease. | four-layer antispecies radioimmunoassay (ria) and enzyme immunoassay (eia) procedures were developed for the detection of respiratory syncytial virus (rsv), parainfluenza type 2 virus, and adenovirus antigens in nasopharyngeal specimens from children hospitalized for acute respiratory disease. polystyrene beads (ria) or flat-bottomed polystyrene microtiter plates (eia) were used as the solid phases, guinea pig anti-virus immunoglobulins were used as the captive antibodies, rabbit anti-virus immu ... | 1981 | 6259198 |
| integration of rous sarcoma virus dna during transfection. | we have investigated the organization and integration sites of rous sarcoma virus (rsv) dna in nih 3t3 mouse cells transformed by transfection with unintegrated and integrated donor rsv dnas. rsv dnas of different cell lines transformed by unintegrated donor dna were flanked by different cellular dna sequences, indicating that rsv dna integrates at multiple sites during transfection. the rsv genomes of cells transformed by transfection were colinear with unintegrated rsv dna, except that deletio ... | 1981 | 6260381 |
| sodium and rubidium uptake in cells transformed by rous sarcoma virus. | rates of uptake and intracellular concentrations of monovalent cations were measured in virus-transformed and nontransformed chick embryo (ce) cells. uptake of 22na+ into cells transformed by the bh strain of rous sarcoma virus (rsv-bh) (ce-bh) was about double the rate of uptake into ce cells, or cells transformed by the schmidt-ruppin strain (rsv-sr): ce-sr. likewise, the rate of efflux of 22na+ was greater in ce-bh cells than in ce or ce-sr cells. the greater permeability of ce-bh cells to na ... | 1981 | 6260819 |
| secondary structural features in the 70s rnas of moloney murine leukemia and rous sarcoma viruses as observed by electron microscopy. | the secondary structural features in the 70s rnas of the prague strain of avian rous sarcoma virus, subgroup a (pr-rsv-a), and moloney murine leukemia virus (m-mulv) were compared by electron microscopy. the pr-rsv-a genome contained two subunits joined by a linkage structure as in the genomes of m-mulv and other mammalian retroviruses. in both viral genomes, a highly reproducible hairpin occurred at about 70 nucleotides from the 5' end of each subunit and contained 320 +/- 8 nucleotides. the st ... | 1981 | 6260992 |
| independent expression of avian sarcoma virus in doubly infected chicken embryo fibroblasts. | infection of a chicken cell with avian sarcoma virus requires division of the infected cell before synthesis of infectious progeny is initiated. this requirement for a cell division for the complete expression of avian sarcoma virus has been examined further with chicken embryo fibroblasts infected with two distinct viruses. chicken cells infected with and producing a mutant of rous sarcoma virus temperature sensitive for transformation (tsla24pr-a) were arrested in g0 by depletion of serum fact ... | 1981 | 6261016 |
| decrease in the levels of nuclear rna precursors for alpha 2 collagen in rous sarcoma virus transformed fibroblasts. | we have examined the levels of type i alpha 2 collagen rna precursors, containing both intron and exon sequences in nuclear rna preparations of chick embryo fibroblasts (cef) and of cef transformed by the schmidt-rupin strain of rous sarcoma virus (rsv). we have used two different fragments of chick alpha 2 collagen genomic dna as hybridization probes in s1 mapping experiments. each of these dna probes contains an entire intron. our results indicate that the levels of the primary transcript of a ... | 1981 | 6262721 |
| structural and functional domains of the rous sarcoma virus transforming protein (pp60src). | the transforming protein (pp60src) of the rous sarcoma virus (rsv) is a phosphoprotein with the enzymatic ability to phosphorylate tyrosine in protein substrates. previous work has indicated that the bulk of pp60src may be attached to the plasma membrane of infected cells. in an effort to better understand the mechanism by which pp60src induces the neoplastic phenotype, we have characterized further the attachment of pp60src to the plasma membrane, and we have identified separate molecular domai ... | 1981 | 6262821 |
| regulation or procollagen messenger ribonucleic acid levels in rous sarcoma virus transformed chick embryo fibroblasts. | using cloned cdnas for pro-alpha 1 and pro-alpha 2 collagen messenger ribonucleic acid (mrna), we have investigated the regulation of collagen mrna levels in rous sarcoma virus (rsv) transformed chick embryo fibroblasts (cef). we find that both pro-alpha 1 and pro-alpha 2 mrna levels are decreased approximately 10-fold in cef transformed by either the bryan high-titer strain or the schmidt-ruppin strain of rsv. using temperature-sensitive mutants in the transforming gene src, we also investigate ... | 1981 | 6263317 |
| transforming proteins of some feline and avian sarcoma viruses are related structurally and functionally. | transformation of chicken cells by fujinami sarcoma virus (fsv), prc ii or y73 (three independently isolated avian sarcoma viruses that are replication-defective and lack the rous sarcoma virus src gene) resulted in significant elevation (4-13 fold) of phosphotyrosine levels in cellular protein. the gag-related proteins encoded by these avian sarcoma viruses (asvs) were all associated with tyrosine-specific protein kinase activity when assayed in immune complexes and were phosphorylated at both ... | 1981 | 6263483 |
| vinculin: a cytoskeletal target of the transforming protein of rous sarcoma virus. | vinculin, a protein associated with the cytoplasmic face of the focal adhesion plaques which anchor actin-containing microfilaments to the plasma membrane and attach a cell to the substratum, contains 8-fold more phosphotyrosine in cells transformed by rous sarcoma virus than in uninfected cells. because the transforming protein of rsv, p60src, is a protein kinase that modifies cellular proteins through the phosphorylation of tyrosine and because phosphotyrosine is a very rare modified amino aci ... | 1981 | 6263485 |
| the expression of glial fibrillary acidic protein in a rat cerebellar cell line. | a rat cerebellar cell line, wc5, derived by transformation with rous sarcoma virus, which is temperature-sensitive for transformation (ts-rsv), can be induced to express glial fibrillary acidic protein (gfap). immunofluorescence, radioimmune assay, and electron microscopy studies show that gfap is expressed in wc5 cells grown at the nonpermissive temperature (npt), but not at the permissive temperature (pt) for transformation. gfap is first detectable about 3 days after incubating cells at the n ... | 1981 | 6265476 |
| hybrid plasmids carrying part of the rous sarcoma virus-specific leader sequence. | 'strong-stop' dna, complementary to the 5'-terminal 101 nucleotides of rous sarcoma virus genomic rna and subgenomic mrnas can be used to isolate rsv-specific mrna from infected cells. to analyse leader sequences of these mrnas and to study the mechanism of their translation, relatively large quantities of strong-stop dna are required. the construction of four plasmids carrying useful strong-stop dna sequences for mrna isolation is described. one such hybrid plasmid carries a dna insertion conta ... | 1981 | 6266483 |
| neuraminidase activity and cell surface sialic acid turnover in rous sarcoma virus transformed chick embryo fibroblasts. | neuraminidase activity of rous sarcoma virus transformed chick embryo fibroblasts (rsv-cef) was assayed using an exogenous substrate, neuraminlactitol-[3h], and endogenous, cell surface [14c]-n]-acetyl-neuraminic acid. rsv-cef had higher neuraminidase activity toward both substrates than did chick embryo fibroblasts (cef) or nontransformed, rous associated virus infected cef (rav-cef). the total sialic acid content of rsv-cef was lower than cef or rav-cef, and more of the total sialic acid was a ... | 1981 | 6266645 |
| electron spin resonance of growing normal and virus-transformed cells. | a g = 2.003 esr signal, attributed to a free radical localized in hela cell nuclei and mitochondria but absent in membranes and cytoplasm, has been studied as a function of the culture growth cycle in normal (nrk and 3t3) and virus transformed (nrk/rsv and 3t3/sv40) cells. for both these cell pairs, the signal is higher during the "lag" stage and lower during the "growth" stage. the average specific intensity of the signal in normal cells is about twice that in virus-transformed cells. however, ... | 1981 | 6266648 |
| complementing mhc- and non-mhc-linked genes and resistance to avian sarcoma virus-induced tumours in inbred lines of chickens. | schmidt-ruppin rous sarcoma virus, subgroup b (sr-rsv-b), was inoculated into the wingwebs of chickens from three partially congenic inbred lines, g-b1, g-b2 and g-b3, homozygous for different mhc (b region) haplotypes (genotypes = b1/b1, b2/b2 and b3/b3 respectively). all birds developed tumours but only the g-b2 line resisted progressive tumour growth. birds from lines g-b1 and g-b3 approached 100% susceptibility to progressive tumour growth, whereas most (g-b1 x g-b3) f1 hybrids were resistan ... | 1981 | 6267140 |
| b-haplotype influence on marek's disease, rous sarcoma, and lymphoid leukosis virus-induced tumors in chickens. | experiments were conducted to establish whether b-haplotype genes that determine resistance to rsv-induced tumors would also influence resistance to marek's disease and lymphoid leukosis. matings of line 6(3) x 15(1) f3 or f4 breeders segregating for the ea-b locus antigens that are markers for b2 and b5 haplotypes of the b major histocompatibility complex were made to produce b2b2, b2b5, and b5b5 chicks. the chicks were infected with a standard inoculum of one of the three tumor viruses in sepa ... | 1981 | 6267578 |
| differential effects of transforming avian rna tumor viruses on avian macrophages. | functionally differentiated chicken macrophages were derived by in vitro differentiation of embryonic yolk sac cells and were characterized by several macrophage-specific cell markers. uniform, infected, virus-producing cultures were obtained after exposure of these macrophages to avian myoblastosis virus (amv), avian myelocytomatosis virus (mc29), myeloblastosis-associated virus (mav-2), and prague strain of rous sarcoma virus (pr-b rsv). both amv and mc29 induced morphological transformation t ... | 1981 | 6267600 |
| retroviruses as mutagens: insertion and excision of a nontransforming provirus alter expression of a resident transforming provirus. | integration of retroviral dna appears to occur randomly in host genomes, suggesting that retroviruses can act as insertion mutagens. we have confirmed this prediction by showing that the nontransforming retrovirus, moloney murine leukemia virus (m-mulv), can insert its provirus within the selectable target provided by a single provirus in a clonal rat cell line (b31) transformed by rous sarcoma virus (rsv). analysis of over 60 morphological revertants of m-mulv-superinfected b31 cells revealed t ... | 1981 | 6268304 |
| electrochemical modification of vesicular stomatitis virus glycoprotein by host cell transformation. | electrochemical properties of the glycoprotein of vesicular stomatitis virus (vsv) grown in rous sarcoma virus (rsv)-transformed cells was compared with that of its counterpart grown in nontransformed cells. in deae-sephadex column chromatography, the glycoproteins of vsv derived from transformed cells appeared more heterogeneous and had a tendency to elute with higher concentrations of nacl than those from nontransformed cells. in isoelectric focussing, the glycoproteins of vsvs derived from tr ... | 1981 | 6268946 |
| acute non-bacterial infections of the respiratory tract in singapore children: an analysis of three years' laboratory findings. | a retrospective study of the laboratory results on respiratory specimens received from children under 12 years of age between january 1977 and december 1979 was carried out. these children were either hospital patients, usually with lower respiratory infections, or outpatients on the influenza surveillance programme. the overall virus isolation rate was 26.8%, and the isolation rate among hospital patients, 38.5%. epidemics or outbreaks were associated with infections due to the influenza viruse ... | 1981 | 6269479 |
| helper virus-dependent rsv rescue from two lines of hamster cells transformed in vivo by xc rsv. | newborn hamsters were inoculated with chicken sarcoma tissue produced by xc rsv which had been rescued by transfection from xc cells. tumours with a latency of up to 20 months appeared in all inoculated hamsters. three tumour cell lines were successfully established for in vitro growth and cloned. the virus was rescued from the pr rsh-9 cell line by cell fusion with chicken fibroblasts and from the other two lines (pr rsh-12 and pr rsh-14) and clones only if chicken fibroblasts used for fusion h ... | 1981 | 6270026 |
| comparison of camp-dependent protein kinase in normal and rous sarcoma virus transformed chick embryo fibroblasts. | camp-dependent protein kinase was compared in normal and rous sarcoma virus transformed chicken embryo fibroblasts. total camp binding activity and camp-dependent histone kinase activity were unaltered by rsv transformation. the apparent km for activation of histone kinase activity by camp was 35 nm in both normal and transformed cells. using 8-n3-camp photoaffinity labeling, normal and transformed cells were also found to contain equal quantities of a single 42,000 mr regulatory sub-unit isoenz ... | 1981 | 6270200 |
| lack of correlation between pp60src kinase activity and transformation parameters in cells infected with temperature-conditional mutants of rous sarcoma virus. | the pp60src kinase activity of cells infected with temperature-conditional mutants of rous sarcoma virus (rsv), which induce only a partial transformation, was compared to the pattern of transformation parameters induced by these mutants. the tsgi251-infected cells were thermosensitive for hexose transport, fibrinolysis, morphological alterations and pp60src kinase activity, but cold-sensitive for loss of growth control. the tsgi253-infected cells were similar to tsgi251-infected cells except th ... | 1981 | 6270240 |
| src genes of ten rous sarcoma virus strains, including two reportedly transduced from the cell, are completely allelic; putative markers of transduction are not detected. | the src genes of different rous sarcoma virus (rsv) strains have been reported to be highly conserved by some investigators using rna-cdna hybridization, whereas others using oligonucleotide, peptide, and serological analyses have judged src genes to be variable in 30 to 50% of the respective markers. moreover, distinctive src oligonucleotides and peptides of so-called recovered rsvs (rrsv's) whose src genes were reported to be experimentally transduced from the cell are thought to represent spe ... | 1981 | 6270350 |
| a rous sarcoma virus provirus is flanked by short direct repeats of a cellular dna sequence present in only one copy prior to integration. | the rous sarcoma virus (rsv)-transformed rat cell line rsv-nrk-2 contains a single complete rsv provirus. we have obtained recombinant lambda clones that contain both ends of the rsv provirus and the flanking rat sequences. the provirus is integrated in unique dna and is present in only one of the two homologous chromosomes. the rat sequences into which the rsv provirus integrated were also cloned from the rsv-nrk-2 cell line. the sequences of the regions involved in the recombination event have ... | 1981 | 6270669 |
| biological activity of cloned retroviral dna in microinjected cells. | avian retroviral dna molecules that had been cloned from infected cells by using recombinant dna techniques were microinjected into either uninfected chicken embryo fibroblasts (cef) or cef transformed by the envelope glycoprotein-deficient bryan strain of rous sarcoma virus [rsv(--)cells]. retroviral dna injected into rsv(--) cells directed transcription of envelope mrna, which was then able to complement the rsv(--) env deficiency and promote the production of infectious transforming virus. th ... | 1981 | 6270676 |
| continuous production of rous sarcoma virus free of transformation defective virus in clones of established rsv-transformed quail cells. | 1981 | 6270893 | |
| [acute respiratory syndromes caused by viruses in a pediatric department. clinical and epidemiological studies of a series of cases admitted in the period from february to may, 1979]. | in the period between february and may 1979, in concomitance with a sharp increase in admittance to the pediatric division of the parma general hospital for acute respiratory infections, we carried out a virologic and serologic investigation on hospitalized children aged 21 days-12 years. the investigation was carried out on 137 patients with respiratory diseases: 33 with bronchiolitis, which occurred mainly (32/33) between february and march in children that were prevalently (30/33) in the firs ... | 1981 | 6271082 |
| evidence that viral transforming gene products and epidermal growth factor stimulate phosphorylation of the same cellular protein with similar specificity. | treatment of a-431 human epidermoid carcinoma cells with epidermal growth factor (egf) was shown to enhance the phosphorylation of a mr = 34,000 protein. because the phosphorylation of an analogous protein is enhanced in various cell lines transformed by rous sarcoma virus (rsv) (erikson, e., and erikson, r. l. (1980) cell 21, 829-836), we characterized the phosphorylation of the a-431 mr = 34,000 protein under these two conditions in order to determine whether there are common pathways between ... | 1981 | 6271744 |
| tropomyosin is decreased in transformed cells. | the steady-state level and synthesis of a pair of polypeptides of mr 33,000 and 35,000 in chicken embryo fibroblasts (cef) transformed by rous sarcoma virus (rsv) are significantly decreased relative to normal cef; however, the decrease is more pronounced in the case of the mr 35,000 polypeptide. these polypeptides have been identified as the alpha and beta subunits of cef tropomyosin by selective staining with tropomyosin antibody, two-dimensional gel electrophoresis, partial peptide analysis, ... | 1981 | 6272310 |
| [incorporation of influenza virus antigens into the envelope of the avian oncornavirus]. | the mixture of influenza virus and pseudotype of rous sarcoma virus--rsv (pab-a) grown in chick embryos or cell culture of chick fibroblasts produces a progeny which can be partially neutralized by a heterologous immune serum. the fraction of viral particles, the transformation activity of which is susceptible to suppression by both homologous anti-rsv (pab-a) and heterologous serum, immune to influenza virus, amounts to approximately 20%. after passivation, these particles lose this property wh ... | 1981 | 6272489 |
| [biological properties of the rous sarcoma virus isolated from transformed hamster cells]. | rous sarcoma virus (rsv) isolated from virogenic transformed hamster cells differed from the original schmidt-ruppin strain used to inoculate hamster embryo fibroblasts in the capacity to effectively transform mammalian cells, to multiply in these cells, in the reduced reproductive activity in chicken cells. these altered properties were relatively stable since they were retained in the first virus passages in chickens. | 1981 | 6272496 |
| patterns of shedding of myxoviruses and paramyxoviruses in children. | in the houston family study, young children were cultured for virus weekly or biweekly and during acute respiratory illnesses. the interval between the onset of illness and positive culture was examined for 179 infections during 1975-1979. in week 1 after onset, 73%, 73%, and 66% of cultures were positive for influenza a virus, respiratory syncytial virus (rsv), and parainfluenza virus type 3, respectively. pooled data from influenza b virus infections in 1977 and 1980 showed that 73% of culture ... | 1981 | 6273473 |
| the role of nk cells in tumor growth assessed by induction of their suppressor cells in japanese quails. | in japanese quails treated with chicken amniotic fluid (chamf) which had been previously shown to induce suppressor cells to natural killer (nk) cells, tumors appeared with shortened incubation periods after inoculation with schmidt-ruppin strain of rous sarcoma virus (sr-rsv) compared with untreated quails. the tumors in chamf-treated quails subsequently grew in a similar pattern to those in untreated quails, whereas by challenging with a lower dose of the virus, enhanced tumor growth was obser ... | 1981 | 6273620 |
| inhibition of plasminogen activator release from transformed chicken fibroblasts by a protease inhibitor. | chicken embryo fibroblasts (cef) transformed by rous sarcoma virus (rsv) exhibit increases in both a cell-associated and a secreted form of plasminogen activator (pa). the mechanism whereby the membrane-bound, cell-associated form of pa is processed to an extracellular, soluble form has been examined in cultures of chicken fibroblasts transformed by a temperature-sensitive mutant of rsv. we report that chymostatin, a protease inhibitor of limited specificity, inhibits the release of pa from tsrs ... | 1981 | 6276025 |
| host age and major histocompatibility genotype influence on rous sarcoma regression in chickens. | the influence of host age and b (major histocompatibility complex) genotype on the fate of rous sarcoma virus (rsv)-induced tumors was investigated. white leghorn lines 61 and 151, developed at the regional poultry research laboratory (rprl) at east lansing, mi, and their crosses (f3 to f5) segregating for the b genotype were used. preliminary experiments with noninbred white leghorns showed that chickens rsv-inoculated at 4 weeks of age had a significantly lower incidence of tumor regression th ... | 1981 | 6276876 |
| [gliomas induced in mammals by rous sarcoma virus (rsv) (author's transl)]. | studies concerning the induction of gliomas in a variety of animal species by an oncogenic avian virus (rous sarcoma virus) were presented. experimental conditions (age of inoculation, doses of virus) were reviewed. preliminary attempts to localize viral antigens in the tumors in vivo using labelled antibodies were discussed. | 1981 | 6278340 |
| [comparative ultrastructure of human gliomas and experimental gliomas induced by rous sarcoma virus (rsv) (author's transl)]. | a brief review of earlier results concerning the ultrastructure of 20 gliomas induced by intracerebral inoculation of rous sarcoma virus in the dog emphasizes the similarity of certain canine and human histological types. since no sign of the viral induction is recognizable for certain in the experimental tumors and since the large size of the animal authorizes the use of the same radiological detection techniques as in man these viral induced tumors represent a remarkable experimental model. | 1981 | 6278341 |
| interferon production by human mononuclear leukocytes: differences between respiratory syncytial virus and influenza viruses. | the ability of respiratory syncytial virus (rsv) to induce interferon production by human mononuclear leukocytes was compared with that of influenza viruses. cell culture fluids were assayed for interferon activity 1, 3 and 7 days after exposure to rsv or to one of two subtypes of influenza a virus (h0n1 and h3n2). rsv induced interferon production inconsistently and in low titers. varying the multiplicity of infection did not improve the ability of rsv to induce interferon production. in contra ... | 1981 | 6163726 |
| evidence for a gp85-related glycoprotein in cells transformed by the bryan high titer strain of rous sarcoma virus. | the bryan high titer strain of rous sarcoma virus (bh-rsv) is a deletion mutant in the env-gene coding for the viral envelope glycoproteins gp35 and gp85. in this report experimental evidence is described that cells, transformed by bh-rsv, express a glycoprotein immunologically related to gp85. animals bearing bh-rsv induced tumors produce antibodies reacting with gp85 of nondefective rsv. lysates of a bh-rsv transformed quail cell line, r(-):q, inhibit the immunoprecipitation of gp85 by antibod ... | 1981 | 6168244 |
| a mouse homolog to the avian sarcoma virus src protein is a member of a protein kinase cascade. | recent work has identified a cascade of membrane bound protein kinases in ehrlich ascites tumor cells. these enzymes, designated pkl, pks and pkm, are present in both ehrlich tumor and mouse brain, but the cascade is active only in the tumor tissue. we have now purified a fourth protein kinase, pkf, that is also associated with this cascade. protein kinase f prosphorylates pkl and is phosphorylated by pks. the position of this kinase in the cascade is as follows, where the arrows denote phosphor ... | 1981 | 6168390 |
| the development of respiratory syncytial virus-specific ige and the release of histamine in nasopharyngeal secretions after infection. | we studied the development of respiratory syncytial virus (rsv)-specific ige and the release of histamine in nasopharyngeal secretions from 79 infants with various forms of respiratory illness due to rsv. rsv-ige was measured by an enzyme-linked immunosorbent assay; specificity was confirmed by appropriate blocking experiments. histamine content in the secretions was determined by fluorimetric methods. rsv-ige was detectable in only one of 19 patients with rsv infection without wheezing, but was ... | 1981 | 6168908 |
| mouse fibroblasts transformed by rous sarcoma virus express a virus-specific non-virion transplantation antigen. | rous sarcoma virus (rsv)-transformed fibroblasts from different animals species a serologically detectable virus-induced non-virion cell surface antigen (vcsa), whose expression is controlled by the transforming viral src gene and by a cellular gene. now, by in vivo immunization, we have found that rsv-transformed fibroblasts from different mouse strains share a virus-specific transplantation antigen. in fact, only animals immunized with irradiated syngeneic or allogeneic fibroblasts transformed ... | 1981 | 6169669 |
| [production of noninfectious virions of the rous sarcoma virus by virogenic hamster cells]. | noninfectious virions morphologically identical with avian type c virus virions were produced in rous sarcoma virus-transformed virogenic hamster cells. the population of the virions contained the major internal protein of avian oncornavirus. it is assumed that production of defective rsv virions occurred in the cells. the major internal protein of avian oncornaviruses was found to be incorporated into the virions containing in their membrane interspecies antigens of hamster oncornavirus produce ... | 1981 | 6171103 |
| tumor promoters alter gene expression and protein phosphorylation in avian cells in culture. | we have investigated the effect of 12-o-tetradecanoylphorbol 13-acetate (tpa) on the synthesis and modification of polypeptides in normal avian cells and cells infected by wild-type and temperature-sensitive rous sarcoma virus (rsv). using two-dimensional gel electrophoresis, we have detected alterations in both the abundance of cellular polypeptides and in their phosphorylation that seem unique to tpa treatment. however, the state of phosphorylation of the major putative substrate for the actio ... | 1981 | 6171814 |
| in vitro differentiation of chicken embryo skin cells transformed by rous sarcoma virus. | the epidermal cells isolated from 14-day chicken embryo shank skin epidermis were infected in vitro with rous sarcoma virus (rsv). within a few weeks, rapidly growing colonies of epithelial cells appeared among the sea of transformed fibroblastic cells. when isolated and subcultured, these cells were found to possess typical markers of skin epidermis. the presence of major keratin and typical epithelial cell type morphology strongly suggested that these cells were transformed epidermal cells ret ... | 1981 | 6172432 |
| rous sarcoma virus mutant la3382 is defective in virion glycoprotein assembly. | la3382 is a temperature-sensitive replication-defective mutant of rous sarcoma virus that contains four active mutations. in this study we performed experiments to determine the biochemical defect that blocks the synthesis of infections virus late in the replication cycle. at the nonpermissive temperature (41 degrees c) cells infected with la3382 synthesized virus particles which were noninfectious and exhibited significant reductions in the amounts of gp85 and gp37 present in the virions. an an ... | 1981 | 6172600 |
| similarities and differences between the effects of epidermal growth factor and rous sarcoma virus. | we have derived a line of a431 human tumor cells infected with rous sarcoma virus (rsv). the infected cells contain the rsv-transforming protein, pp60src, which has characteristic tyrosine specific protein kinase activity. as in other rsv-transformed cells, a 36,000-dalton protein is phosphorylated in rsv-infected a431 cells. addition of epidermal growth factor (egf) to the cells induces further phosphorylation of this protein. in contrast, this phosphoprotein is not detected in uninfected a431 ... | 1981 | 6173387 |
| [rous virus-induced sarcoma study of macaca mulatta monkeys]. | the carr-zilber strain of rous sarcoma virus is highly oncogenic for adult monkeys of the species macaca mulata. in the present study the authors examined some characteristics of tumor growth, when the tumor either regressed or progressed until it killed its host. the kinetics of antibody formation to antigens coded as cz-rsv was followed in animals with a different course of tumor disease. the sera of animals under test contained virus-neutralizing antibodies in addition to gs antibodies. | 1981 | 6256976 |
| pneumonia: an eleven-year study in a pediatric practice. | data from an 11-year study of acute lower respiratory tract illness were analyzed to provide added insight into the causes and patterns of occurrence of pneumonia in an outpatient pediatric population. over 80% of all episodes of pneumonia occurred in children less than seven years of age; the peak attack rate was observed in 2-4-year-old children. a virus or mycoplasma pneumoniae was isolated from 24% of children with pneumonia; 57% of all episodes occurred during outbreaks for which a viral or ... | 1981 | 6257108 |
| maize stripe virus: characteristics of a member of a new virus class. | an unusual filamentous nucleoprotein about 3 nm in diameter was consistently associated with maize stripe-diseased maize. antiserum to purified nucleoprotein neutralized the infectivity of extracts from maize stripe-diseased plants suggesting that the nucleoprotein was the maize stripe virus (mstpv). the rate-zonal sedimentation pattern of the nucleoprotein on sucrose gradients was polydisperse between 51 and 70 s. cscl isopycnic centrifugation of combined nucleoprotein zones from sucrose gradie ... | 1981 | 18635064 |
| age related resistance to avian leukosis virus. iii. infectious virus, neutralising antibody and tumours in chickens inoculated at various ages. | viraemia and neutralising antibodies were determined in chickens of six age-groups following inoculation with leukosis virus of subgroups a and b at the age of 1 day, and 2, 4, 6, 8 and 10 weeks respectively. the birds were kept in a filtered air positive pressure (fapp) house. a seventh age-group, accommodated in a separate fapp-house, was used as an untreated control. serum samples, received at biweekly intervals between 1-17 weeks post-inoculation, from birds of the groups inoculated at 4, 6, ... | 1982 | 18770194 |
| identification of phosphotyrosine-containing proteins in untransformed and rous sarcoma virus-transformed chicken embryo fibroblasts. | phosphorylation on tyrosine residues mediated by pp60src appears to be a primary biochemical event leading to the establishment of the transformed phenotype in rous sarcoma virus (rsv)-infected cells. to identify the cellular proteins that undergo tyrosine phosphorylation during transformation, a 32p-labeled rsv-transformed chicken embryo cell extract was analyzed by electrophoresis on a polyacrylamide gel. after slicing the gel into approximately 60 slices, phosphoamino acid analyses were carri ... | 1982 | 14582160 |
| electron microscopic studies on the structure of 60-70s rna of avian myeloblastosis virus. | structural properties of the 60-70s rna complex of avian myeloblastosis virus (amv) were analysed in electron microscope after treatment under a set of non-denaturing, gently and strongly denaturing conditions. by selected denaturing conditions, the significant fraction of 60-70s amv rna molecules revealed partially unfolded structures either in a dimer or a more complex form and in a length corresponding to mol. wt. of 5.6 x 10(6). the typical dimers contained a characteristic central structure ... | 1982 | 6132536 |
| monoclonal antibody against the carboxy terminal peptide of pp60src of rous sarcoma virus reacts with native pp60src. | a monoclonal mouse antibody has been prepared against a synthetic peptide corresponding to the six carboxy-terminal amino acids (c' peptide) of the src gene product pp60v -src of rous sarcoma virus (rsv). the antibody was able to precipitate pp60v -src and to bind pp60v -src kinase activity in a competition test, indicating that this peptide can serve as an antibody-binding site (epitope). furthermore, the finding that three out of 28 pp60src-specific tumor-bearing rabbit (tbr) sera contained an ... | 1982 | 6202510 |
| dissection of the antigenic determinants expressed on the cell surface of rsv-transformed fibroblasts by monoclonal antibodies. | fusion of p3/x63-ag8 mouse myeloma cells with splenocytes obtained from mice hyperimmunized with a bhk hamster fibroblast line transformed by an env-strain of rous sarcoma virus (rsv) resulted in the production of antibody-secreting hybridomas. seven hybrid clones secreted antibodies binding to rsv-transformed bhk fibroblasts but not to the parental control non-transformed line. the antibodies produced by three of these clones did identify antigenic determinants expressed also on bhk cells trans ... | 1982 | 6177648 |
| participation of subgenomic retroviral mrnas in recombination. | envelope glycoprotein (env) mrna from avian retroviruses was injected into cells transformed by env-deleted bryan rous sarcoma virus [rsv(-)]. the genetic deficiency of rsv(-) was complemented, and infectious transforming virus was released for many days after these injections. the long-term activity of the injected env mrna is believed to be due to reverse transcription of the injected rna after its incorporation into virus particles. the resulting subgenomic provirus, presumed to be integrated ... | 1982 | 6178841 |
| discrete primary locations of a tyrosine-protein kinase and of three proteins that contain phosphotyrosine in virally transformed chick fibroblasts. | we have studied the localization of three abundant cellular proteins which are substrates for tyrosine protein kinases in virally transformed chicken embryo fibroblasts. the primary location of each substrate is unaltered by transformation with rous sarcoma virus (rsv). the tyrosine-phosphorylated species is localized with the nonphosphorylated species. two of the proteins, of about 46,000 and 28,000 daltons, have a similar location. they are present in the high speed supernatant of cells homoge ... | 1982 | 6179948 |
| interferon inhibits establishment of fibroblast infection with avian retroviruses. | pretreatment of chick embryo fibroblasts (cef) with low doses of homologous interferon (16 u/ml) drastically inhibits cell transformation by, and replication of rous sarcoma virus (rsv). treatment of chick cells with 16 u/ml of interferon before de novo infection with a transformation defective (td) mutant-rsv, also resulted in a reduction of extracellular virus particles. this was determined by infectivity titrations, virus associated reverse transcriptase (rt) activity and measurement of metab ... | 1982 | 6180104 |
| cells transformed by a wide variety of agents express higher abundance levels of some cellular rna species. | a cdna-cloned library was prepared from mrna synthesized by sv40-transformed mouse cells. eleven cdna clones were selected based on their ability to hybridize higher levels of mrna in sv40-transformed 3t3 cells than in 3t3 cells. these cdna clones were employed to screen the steady-state levels of cytoplasmic rnas in a wide variety of viral (sv40, polyoma, adenovirus, and rous sarcoma virus) and nonviral (methylcholanthrene, embryonal carcinoma) transformed cell lines. two of the cdna clones--a1 ... | 1982 | 6185512 |
| expression of neuronal markers in chick and quail embryo neuroretina cultures infected with rous sarcoma virus. | cultures of neuroretina (nr) cells from 7-day chick and quail embryos were infected with ts ny-68, a thermosensitive mutant of rous sarcoma virus (rsv) which transformed nr cells at 36 degrees c. the following differentiation markers for neurones were studied: tetanus toxin-binding sites at the cell surfaces, presence of synapses, and the specific activity of the enzymes choline acetyltransferase (cat) and glutamic acid decarboxylase (gad). appearance of synapses and expression of cat were simil ... | 1982 | 6279316 |