Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| interspecies determinants of friend leukemia virus antigens involved in cytolysis of virus-pfoducing cells. | the cytolytic reactivity of a complex goat anti-feline leukemia virus (felv) antiserum for mouse cells (eveline) releasing large quantities of friend leukemia virus (flv) was analyzed by the sensitive [14c]nicotinamide release microcytotoxicity assay. whereas this interspecies killing reactivity could be blocked by absorption of the goat anti-felv serum with a preparation of disrupted flv, absorption with purified flv gp71, the major envelope glycoprotein, had no effect. subsequent serum absorpt ... | 1977 | 64623 |
| cells transformed by certain strains of moloney sarcoma virus contain murine p60. | it was previously demonstrated that the 60,000 dalton (p60) precursor-like polyprotein containing murine p30 was a constituent of the feline leukemia virus pseudotype of moloney sarcoma virus [m1msv(felv)]. it is now shown that p60 is detected in cells of five mammalian species transformed by m1msv, indicating that p60 is specified by this genome. moreover, little or no murine p30 is detected in the m1msv-transformed cells, suggesting that the murine group p30 antigenic reactivity of s + l- cel ... | 1977 | 65230 |
| expression of antigenic crossreactivity to rd114 p 30 protein in a human fibrosarcoma cell line. | an antigen crossreacting with the 30,000-molecular-weight protein (p30) of the feline endogenous oncornavirus (rd114) was detected in a well-characterized human fibrosarcoma cell line, ht1080, by indirect immunofluorescence. three antisera against rd114 p30 gave similar positive results, while two antisera prepared against simian sarcoma virus p30, one antiserum prepared against murine leukemia virus p30, and one antiserum prepared against feline leukemia virus p30 gave no immunofluorescence. th ... | 1977 | 66679 |
| abrogation of resistance to feline oncornavirus disease by immunization with killed feline leukemia virus. | four-week-old specific-pathogen-free cats were immunized with a combined vaccine composed of killed feline leukemia virus and killed feline oncornavirus-associated cell membrane antigen-containing tumor cells. immunization induced feline oncornavirus-associated cell membrane antigen antibody titers ranging from 1:32 to 1:256 but did not elicit detectable virus-neutralizing antibody titers. kittens immunized with tumor cells alone developed higher feline oncornavirus-associated cell membrane anti ... | 1977 | 67886 |
| morphologic and immunologic studies of human prostatic carcinoma. | benign and malignant prostatic tissue was removed in surgery and partitioned for (a) ultrastructural study, (b) tissue culture, and (c) (c) immunochemical study. fourteen malignant and 18 benign prostatic cancer specimens were examined by transmission electron microscopy (tem) for the presence of viruses or virus-like particles. viruses could not be identified with assurity in thin sections. acinar cells of normal, benign prostatic hypertrophy (bph), and neoplastic prostate tissue were examined ... | 1977 | 68819 |
| mammalian c-type oncornaviruses: relationships between viral structural and cell-surface antigens and their possible significance in immunological defense mechanisms. | 1977 | 68853 | |
| horizontal transmission of feline leukemia virus under natural conditions in a feline leukemia cluster household. | ten post-weanling 4-month-old cats, designated "tracers", were placed in a feline leukemia cluster household to determine the efficiency of horizontal transmission of feline leukemia virus (felv). the tracer cats were confirmed as negative for prior exposure to felv. following the placement in the leukemia cluster environment, the tracer cats were serologically monitored at intervals of 3-6 weeks for a total period of 1 year. the tests employed included the detection of felv using fixed-cell imm ... | 1977 | 188773 |
| contained indirect viable-cell membrane immunofluorescence microassay for surface antigen analysis of cells infected with hazardous viruses. | a microtechnique for an indirect viable-cell membrane immunofluorescence titration assay was developed using friend murine leukemia virus (f-mulv)-producing cells and monospecific rabbit antisera to f-mulv structural antigens. the assay was sensitive and displayed little variation within or between assays. since moderate-risk tumor viruses, such as recently discovered primate oncornaviruses or feline leukemia virus (felv), may be hazardous to laboratory personnel, the assay was adapted for conta ... | 1977 | 188865 |
| virus isolation test for feline leukemia virus. | a method was developed for detecting feline leukemia virus (felv) infection in cats by coculturing separated blood lymphocytes and platelets with clone 81 cells carrying a murine sarcoma genome but free of leukemia virus (s+l-). felv caused cell transformation that was easily visible 3-12 days after infection. this method is simple, sensitive, and easily read; it has the advantage of directly detecting infectious felv rather than viral antigens. | 1977 | 189037 |
| detection of complement-dependent lytic antibodies in sera from feline leukemia virus-infected cats by the chromium-51 release assay. | sera from feline leukemia virus-infected cats lysed fl74 cells in the presence of an appropriate complement source; lysis was detected with the use of the 51 cr release method. serum complement from both rabbits and guinea pigs mediated lysis. partial correlation of results was obtained from preliminary comparisons of lysis and immunofluorescence tests with the use of fl74 cells as targets. | 1977 | 189040 |
| horizontal transmission of feline leukemia virus under experimental conditions. | thirty-seven specific-pathogen-free (spf) cats ranging from newborn to 1 year were inoculated with the rickard strain of feline leukemia virus (felv). each inoculated cat shared a cage with a control spf cat for 40 weeks post inoculation. after 4-5 weeks, 20 of the inoculated cats became group-specific antigen (gsa)-positive; the other 17 remained gsa-negative but developed virus neutralizing and feline oncornavirus cell membrane-associated antigen antibody titers. seventeen of the control cats ... | 1977 | 189052 |
| active and passive immunization of cats with inactivated feline oncornaviruses. | 1977 | 198563 | |
| isolation of feline leukemia virus from clinical specimens. | specimens obtained from feline leukemia virus (felv)-positive cats were examined for infectious felv. feline leukemia virus was detected by a focus-forming assay and confirmed by florescent antibody. techniques of sample processing were evaluated and adjusted for optimum detection of felv. low levels of felv were detected in 2 of 10 oral samples; however, the majority of these samples (17 of 27 tested) produced cytopathic effects in tissue culture which prevented fe lv detection. three of 24 uri ... | 1977 | 189647 |
| preparation of cell-free feline oncornavirus-associated cell membrane antigen. | cell-free feline oncornavirus-associated cell membrane antigen (focma) was prepared from a feline lymphoblastoid cell line of tumor origin (fl-74). membrane fractions, separated on sucrose density gradients, and papain-solubilized products were found to contain focma as determined by their capacity to inhibit reference cytotoxic cat antisera. | 1977 | 190418 |
| felv epidemiology in los angeles cats: appraisal of detection methods. | we investigated the reliability of the fixed cell indirect fluorescent antibody (ifa) peripheral blood smear test as an index of systemic infection with felv. positive results with this test were found to correlate well with detectable felv p30 antigen in bone-marrow smears by ifa, in serum and tissue by competition immunoassays, and with type-c particles in bone marrow or spleen by electron microscopy. most cats with lymphoma, anemia or infectious peritonitis were positive for felv and showed a ... | 1977 | 191415 |
| retinal neoplasia and dysplasia. i. induction by feline leukemia virus. | feline leukemia virus, a naturally occurring c-type virus (oncorna virus), was injected systemically and intraocularly into fetal and newborn kittens. a light microscopy study of the resultant ocular tumor changes was carried out. one animal developed an intraocular tumor of apparent retinal origin. eight animals showed intraocular neoplastic cell infiltration. the most common ocular change observed was retinal dysplasia. sequential histologic examination of enucleated eyes showed diffuse intrao ... | 1977 | 191424 |
| studies of naturally transmitted feline leukemia virus infection. | 1977 | 201187 | |
| feline oncornavirus-associated cell-membrane antigen (focma): distinction between focma and the major virion glycoprotein. | the humoral antibody response of feline leukemia virus (felv)-exposed cats to the feline oncornavirus-associated tumor cell-membrane antigen (focma) is directly correlated with immunosurveillance against tumor development under natural conditions. by means of membrane immunofluorescence and radioimmunoprecipitation, the antibody response to focma was found to be independent of the antibody response to the major envelope and core proteins of felv, gp70 and p30. this was especially true for health ... | 1977 | 191832 |
| the epidemiology of the feline leukemia virus (felv). | clustering of cases of feline lymphosarcoma (lsa) has been observed by veterinarians for many years. in 1964 it was discovered that feline lsa was caused by an oncornavirus, the feline leukemia virus (felv). in 1970, a simple, indirect immunoflourescent antibody (ifa) test for felv was developed which enabled large numbers of cats, living in their natural (household) environments, to be tested for the virus. in one study, over 2,000 cats were tested and the results showed conclusively that felv ... | 1977 | 192437 |
| purification of infectious feline leukemia virus from large volumes of tissue culture fluids. | feline leukemia virus from tissue culture fluids was concentrated 100 times by continuous-flow molecular filtration. virus concentrates retained 100% of their original infectivity. further purification was accomplished by a single sucrose density gradient centrifugation. | 1977 | 192759 |
| analysis of intracellular feline leukemia virus proteins ii. generation of feline leukemia virus structural proteins from precursor polypeptides. | the synthesis and processing of feline leukemia virus (felv) polypeptides were studied in a chronically infected feline thymus tumor cell line, f-422, which produces the rickard strain of felv. immune precipitation with antiserum to felv p30 and subsequent sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) were used to isolate intracellular felv p30 and possible precursor polypeptides. sds-page of immune precipitates from cells pulse-labeled for 2.5 min with [35s]methionin reve ... | 1977 | 192917 |
| rd-114 and feline leukaemia virus genome expression in natural lymphomas of domestic cats. | 1977 | 193031 | |
| prevention of oncornavirus-induced sarcomas in cats by treatment with antiviral antibodies. | 1977 | 193042 | |
| defectiveness of a feline sarcoma virus demonstrable by the acquisition of a new envelope antigenic type by phenotypic mixing. | 1977 | 193272 | |
| interspecies homology of rna tumor virus proteins. | we report the application of a highly sensitive column chromatographic technique to the comparison of tryptic peptide maps of some rna tumor virus proteins. by combining microbore ion-exchange chromatography with a sensitive fluorescent assay using o-phthalaldehyde, we obtained high-resolution peptide maps starting with only microgram amounts of protein. our discovery of coincident peptides from the 15,000 and 30,000 molecular weight proteins from murine and feline leukemia viruses supports sero ... | 1977 | 193551 |
| feline oncornavirus-associated cell membrane antigen: expression in transformed nonproducer mink cells. | the feline oncornavirus-associated cell membrane antigen (focma) is a target for naturally occurring immunity that protects the cat against development of fibrosarcoma and leukemia. feline sarcoma virus-transformed "nonproducer" mink cells express high levels of focma, but not the major viral structural proteins. transformation of the same cells by murine sarcoma virus, or infection with feline leukemia virus, which is nontransforming for epithelial or fibroblastic cells, did not induce focma. t ... | 1977 | 194310 |
| rejectability of virus-induced tumors and nonrejectability of spontaneous tumors: a lesson in contrasts. | in contrast to the excellent surveillance against tumors induced by the ubiquitous viruses in their natural host species, spontaneous tumors developing naturally, without experimental interference, evoke little or no rejection reaction. this may be viewed in relation to the natural history of tumors. in most cases, tumors evolve in several steps, with a successful of clonal variants following each other, characterized by increasing independence of host regulations (including immune restrictions) ... | 1977 | 194366 |
| immunity to leukemia, lymphoma, and fibrosarcoma in cats: a case for immunosurveillance. | 1977 | 194751 | |
| passive immunity to feline leukemia: evaluation of immunity from dams naturally infected and experimentally vaccinated. | antibodies against feline leukemia virus (felv) and the feline oncornavirus-associated cell membrane antigen (focma) were transferred from pregnant cats to their suckling kittens. all of these kittens were protected against infection and oncogenesis by virulent felv when challenged at 2 weeks of age. suckling kittens acquired 25 to 100% of maternal virus-neutralizing and focma titers by 3 days of age, and titers underwent linear decay to undetectable levels by 2 to 3 months of age. focma antibod ... | 1977 | 194840 |
| antibodies from healthy cats exposed to feline leukemia virus lyse feline lymphoma cells slowly with cat complement. | 1977 | 196021 | |
| sizes and concentrations of several type c oncornaviruses and bacteriophage t2 by the resistive-pulse technique. | viruses above about 60 nm in diameter may be rapidly sized to a few nanometers in their natural hydrated state as they pass one by one through a single pore in a newly developed nanometer-particle analyzer based on the resistive-pulse technique of the coulter counter and the use of submicron diameter pores made by the nuclepore process. size measurements for several type c oncornaviruses are: rauscher murine leukemia, 122.3 +/- 2 nm; simian sarcoma, 109.7 +/- 3 nm; mason-pfizer monkey, 140.0 +/- ... | 1977 | 196107 |
| effect of helper virus on the number of murine sarcoma virus dna copies in infected mammalian cells. | cell lines of four mammalian species were each examined for the number of moloney murine sarcoma virus (m-msv) dna copies in total cellular dna after m-msv transformation. sarcoma-positive, leukemia-negative (s+l-) m-msv-transformed cells were compared to m-msv-transformed cells infected with a replicating leukemia virus. both unfractionated m-msv complementary dna and complementary dna representing the msv-specific and the msv-murine leukemia virus-common regions of the m-msv genome were hybrid ... | 1977 | 197257 |
| endogenous rd-114 virus genome expression in malignant tissues of domestic cats. | endogenous xenotropic cat type c virus (rd-114)- and infectious feline leukemia virus (felv)-specific gene expressions were measured in spontaneous sarcomas carcinomas, and nonmalignant cat tissues by molecular hybridization for virus-specific rna and competition radio-immunoassays for the major internal protein (p30) of these two viruses. the results indicate that rd-114 gene expression in sarcomas and carcinomas at both rna and p30 levels is significantly higher than histologically normal tiss ... | 1977 | 197261 |
| feline leukemia virus: biochemical and immunological characterization of gag gene-coded structural proteins. | the major non-glycosylated structural proteins of feline leukemia virus have been isolated, and competition immunoassays have been developed for each. these proteins include the 27,000- to 30,000-molecular-weight major internal antigen designated p30, a 15,000-molecular-weight protein (p15), an acidic protein of 12,000 molecular weight (p12), and a highly basic 10,000-molecular-weight protein (p10). immunologically and biochemically corresponding proteins of feline and murine leukemia viruses ha ... | 1977 | 197262 |
| inhibition of normal lymphocyte mitogenic reactivity by serum from feline leukemia virus-infected cats. | the effect of serum from 12 cats with lymphosarcoma induced by feline leukemia virus (felv) on the response of normal cat peripheral blood lymphocytes to phytomitogen-induced blastogenesis was studied. the majority of felv sera, when tested at a concentration of 20% of the incubation medium, caused a 40 to 70% reduction in the mean blastogenic response to concanavalin a compared to the response obtained with a similar concentration of normal feline serum. results with pokeweed mitogen were simil ... | 1977 | 198125 |
| [feline leukaemia or lymphosarcoma (author's transl)]. | 1977 | 188210 | |
| an immunologic approach to the treatment of cancer. | 1977 | 191975 | |
| feline leukemia virus. its related diseases and control. | 1977 | 191976 | |
| detection and evaluation of feline oncornavirus-induced cell surface antigen(s) shed from cells in vitro. | a method for preparation of soluble feline oncornavirus-induced cell surface antigens was described. this technique relied upon the natural release of antigen(s) from fl-74 feline lymphoblastoid cells during their maintenance at 37 degrees in serum-deficient medium. when concentrated and clarified spent medium from 4-day cultures was tested for its antigen content by inhibition of humoral cytotoxicity, it was found that this natural production of soluble antigen provided more feline oncornaviru ... | 1977 | 198129 |
| [comparative study of oncornavirus type d polypeptides]. | 1977 | 198961 | |
| protection of kittens from feline leukaemia virus infection by maternally-derived antibody. | 1977 | 199981 | |
| inhibition of concanavalin a stimulation of feline lymphocytes by inactivated feline leukemia virus. | in a lymphocyte blast transformation assay, the response of feline lymphocytes to concanavalin a was suppressed 20 to 65 percent in the presence of inactivated feline leukemia virus. the decrease was not due to viral cytotoxicity, as determined by trypan blue viability counts, nor was the virus binding the concanavalin a and interfering with its mitogenic stimulation. the virus may be biochemically repressive in itself, interfering with cell-mediated immunity within the feline system. | 1977 | 200353 |
| relationship between feline leukemia virus antigen expression and viral infectivity in blood, bone marrow, and saliva of cats. | correlation was greater than 90% between feline leukemia virus (felv), group-specific antigen (gsa) in leukocytes, and viral infectivity (vi) in serum or plasma from 132 cats infected with either the rickard strain of felv, the snyder-theilen strain of feline sarcoma virus, or field strains of felv. detection of gsa in blood cells was at least as sensitive as detection of vi in serum. in 45% of felv gsa-positive cats inoculated with felv-rickard strain, vi was detected in saliva. no saliva sampl ... | 1977 | 198120 |
| a feline leukaemia virus- and sarcoma virus-induced tumour-specific antigen. | 1977 | 201856 | |
| excretion of feline leukaemia virus by naturally infected pet cats. | 1977 | 201852 | |
| immuno-prophylaxis and -therapy of c-type oncorna viral diseases in mice and cats. | 1977 | 202852 | |
| feline oncornavirus-associated cell membrane antigen: evidence for an immunologically crossreactive feline sarcoma virus-coded protein. | the feline oncornavirus-associated cell membrane antigen (focma) acts as a target for natural immuno-surveillance against tumor development in the cat. in the present study, mink and rat cells nonproductively transformed by feline sarcoma virus (fesv) were shown to express focma as well as 5'-terminal feline leukemia virus (felv) gag gene proteins, p15 and p12. in contrast, such cells lack detectable levels of other felv gag gene-coded proteins or the env gene product, gp70. focma, p15, and p12 ... | 1977 | 202959 |
| transmissibility of animal cancer viruses. | 1977 | 200591 | |
| immunologic characterization of gp70 and gp45 from rauscher murine leukemia virus. | the envelope glycoproteins gp70 and gp45 were purified from rauscher leukemia virus and compared in radioimmunoassay and gel diffusion using antibody to the purified proteins and a cross-reactive antibody prepared against feline leukemia virus. previous studies indicated that gp70 and gp45 had very similar polypeptide components and differed mainly in carbohydrate content. the current studies indicate that gp70 contains the major antigenic determinants of gp45, and, in addition, determinants uni ... | 1977 | 18625484 |
| reiteration frequency of feline type c viral genomes in homologous and heterologous host cell dna. | differences in the multiplicity of proviral sequences of two feline endogenous viruses, rd-114 and felv, in homologous and heterologous host dna were examined by viral cdna hybridization using cellular dna fractionated with respect to reiteration frequency. the endogenous proviral dnas in cat cells were fractionated predominantly with intermediate-repeated sequences, while rd-114 proviral dna in virus-infected human cells was fractionated together with unique-sequence dna. these differences were ... | 1978 | 201591 |
| the occurrence of feline leukaemia virus neutralizing antibodies in cats. | 1978 | 212375 | |
| characterisation of a tumour-specific antigen on the surface of feline lymphosarcoma cells. | 1978 | 212681 | |
| characterization of a feline sarcoma virus-coded antigen (focma-s) by radioimmunoassay. | a radioimmunoassay has been developed that detects a unique antigen encoded by the genome of the feline sarcoma virus (fesv). pseudotype viral particles containing an fesv-specific polyprotein (p85) were used both as a source of antigen and to prepare specific antisera in rabbits. because p85 contains antigens related to two structural proteins (p15 and p12) of feline leukemia virus (felv), antibodies directed to these were adsorbed with purified felv proteins. the adsorbed rabbit antiserum boun ... | 1978 | 212755 |
| diseases transmitted from pets to man: an evolving concern for veterinarians. | pets are not a major source of human infections but they can transmit certain diseases to man. this transmission usually is complex, requiring close contact with pets or their excretions and frequently involves a breach of sound hygienic practice. in some instances, pathogens of animal origin are acquired inadvertently because infectivity can persist after evidence of gross contamination has gone. veterinarians participate in controlling zoonotic diseases by encouraging rabies vaccination and h ... | 1978 | 213237 |
| leukemia and lymphoma: infrequent manifestations of common viral infections? a review. | horizontally transmitted viruses have been etiologically linked to leukemia and lymphoma in several higher mammals (gibbons, cows, and cats). in the best-studied example, the cat, feline leukemia virus is a common community-acquired virus that infrequently produces cancer. however, the infection and its complications (cancer) are not typical of infectious diseases. epidemilogically, serologically, and virologically, infections with this type of virus can contradict classic infectious disease dog ... | 1978 | 216756 |
| recent developments in feline disease research. | 1978 | 204451 | |
| detection of feline leukemia virus in tissues of cats by a paraffin embedding immunofluorescence procedure. | 1978 | 213612 | |
| feline leukemia virus envelope glycoprotein vaccine: preparation and evaluation of immunizing potency in guinea pig and cat. | 1978 | 214605 | |
| research--the lifeline of veterinary medicine. | 1978 | 216897 | |
| naturally occurring acquired immunodeficiency diseases of the dog and cat. | 1978 | 220770 | |
| applied viral immunology. | 1978 | 220771 | |
| immunologic methods for the detection of humoral and cellular immunity. | a variety of immunologic techniques have been introduced during the past few years. many of these techniques are being applied to clinical specimens in an attempt to help the practicing veterinarian make a diagnosis. the introduction of new techniques requires extensive testing with clinically normal and diseased patients. it is essential for the practicing veterinarian to understand that the techniques available for the detection of immunologic disorders in the dog and cat are not routine diagn ... | 1978 | 220772 |
| feline preleukemia: an animal model of human disease. | 1978 | 220806 | |
| the diagnostic laboratory and its relationship to practitioners. diagnostic laboratory professional staff. | diagnostic services provided to practitioners include necropsy, histopathology, bacteriology, virology, parasitology, immunopathology, clinical pathology and toxicology. services are also available at the college in avian and aquatic diseases. the laboratory expects to continually upgrade its services through the addition of appropriate tests, re-evaluation of existing tests, development of methods for more convenient specimen collection and shipment, increased responsiveness to veterinarians' n ... | 1978 | 204452 |
| small animal geriatric and pediatric medicine. | 1978 | 204453 | |
| the control of lymphosarcoma/leukaemia and feline leukaemia virus. | 1978 | 213655 | |
| presence of 5'-terminal cap structures in virus-specific rna from feline leukemia virus-infected cells. | the f-422 line of feline thymus tumor cells, chronically infected with the rickard strain of feline leukemia virus (r-felv), was labeled with 32p, and the total cytoplasmic rna was isolated. the rna was centrifuged through sucrose gradients, and r-felv virus-specific rna (vrna) was located by hybridization of portions of the gradient fractions to r-felv complementary dna. vrna classes with average sedimentation coefficients of approximately 36s, 28s, 23s, and 15s were identified. each class of r ... | 1978 | 207884 |
| seroepidemiologic assessment of feline leukaemia virus infection risk for man. | 1978 | 207994 | |
| influence of antisera to oncornavirus glycoprotein (gp71) on infections of cats with feline leukemia virus. | 1978 | 208242 | |
| differential growth and transmission in cats of feline leukaemia viruses of subgroups a and b. | the outcome of infecting cats with felv of sub-groups a (felv-a) or b (felv-b) was different. after felv-a infection, virus was quickly recovered from the blood and oropharynx of most animals. following infection with felv-b, only a small proportion of cats developed a viraemia, and this after a long interval. there was no evidence that felv-b was transmitted by contact. the result of infecting cats with mixtures of felv-a and b (felv-ab) was that to a large extent each virus operated independen ... | 1978 | 208983 |
| the specificity of neutralizing antibodies to feline leukaemia viruses. | feline leukaemia viruses (felv) of subgroups a (felv-a), b(felv-b) and c(felv-c) were examined by neutralization. the felv-a isolates were monotypic. by contrast, there was antigenic variation within subgroups b and c. there was also considerable cross-reactivity between subgroups. thus, felv-a viruses showed weak cross-reactivity to the standard felv-c isolate; four of the subgroup b viruses were identical and cross-reactied with the standard felv-c, but not with felv-a or the fifth felv-b, and ... | 1978 | 208991 |
| abrogation of lymphocyte blastogenesis by a feline leukaemia virus protein. | 1978 | 209338 | |
| a portion of the feline leukaemia virus genome is not endogenous in cat cells. | although viral sequences closely related to feline leukaemia virus are represented in multiple copies in cellular dna of all domestic cats, a specific fraction was present only in the virus-infected cells. this fraction was detected by viral cdna enriched by a prior absorption of a total complementary dna (cdna) transcript with normal cat liver dna. the recycled cdna hybridized well with the cellular dna of virus-infected cells, but to a lesser extent with dna from uninfected cat cells. the prob ... | 1978 | 210130 |
| potentiation of the neurtalization of feline leukaemia virus in hypotonic medium. | the neutralization of feline leukaemia virus could be enhanced by performing the reaction in hypotonic medium. absorption of antibody by virus specific antigens was also made more sensitive under these conditions. | 1978 | 211186 |
| [control of lymphosarcoma or leukaemia in cats and of feline leukaemia virus (author's transl)]. | a removal programme, specially designed for catteries or multiple cat households, to reduce the incidence of lymphosarcoma or leukaemia in cats and the spread of feline leukaemia virus (felv), is discussed. this removal programme includes annual testing of male stud cats, testing all contacts of felv-positive cats they had during the past two years, testing cats imported from abroad and isolation--or, if this is not possible, euthanasia--of cats found to be positive for felv-antigen. to detect t ... | 1978 | 211674 |
| lysis of feline lymphoma cells by complement-dependent antibodies in feline leukemia virus contact cats. correlation of lysis and antibodies to feline oncornavirus-associated cell membrane antigen. | 1978 | 203702 | |
| cat leukemia and the cluster controversy. | 1978 | 204405 | |
| the frequency of occurrence of feline leukaemia virus subgroups in cats. | feline leukaemia viruses (felv) were isolated from cats in glasgow and new york with lymphosarcoma and from apparently healthy carrier cats. the subgroup composition of each isolate was determined. all isolates contained felv of subgroup a(felv-a) and a high proportion also contained subgroup b virus (felv-b). virus of subgroup c (felv-c) was rare and occurred in association with felv-a and, in some isolates, with felv-b as well. the same pattern was observed in isolates from british and america ... | 1978 | 204584 |
| increased susceptibility to feline leukemia virus infection in cats exposed to methylnitrosourea. | exposure of adult specific-pathogen-free cats to methylnitrosourea resulted in increased susceptibility to infection by feline leukemia virus. a greater proportion of cats exposed to methylnitrosourea and feline leukemia virus (69%) became persistently viremic than those exposed to feline leukemia virus alone (17%). segmented neutrophils were reduced by 90 to 99% within 3 days following exposure to methylnitrosourea, (15 to 20 mg/kg) whereas the effects on lymphocytes and erythrocytes, although ... | 1978 | 205356 |
| analysis of cytoplasmic rna and polyribosmomes from feline leukemia virus-infected cells. | cytoplasmic virus-specific rna and polyribosomes from a chronically infected feline thymus tumor cell line, f-422, were analyzed by using in vitro-synthesized feline leukemia virus (rickard strain) (r-felv) complementary dna (cdna) probe. by hybridization kinetics analysis, cytoplasmic, polyribosomat, and nuclear rnas were found to be 2.1, 2.6, and 0.7% virus specific, respectively. size classes within subcellular fractions were determined by sucrose gradient centrifugation in the presence of di ... | 1978 | 205670 |
| the feline leukemia virus question. | 1978 | 206528 | |
| pseudotypes of feline sarcoma virus contain an 85,000-dalton protein with feline oncornavirus-associated cell membrane antigen (focma) activity. | feline sarcoma virus (fesv) rescued from transformed nonproducer mink or rat cells contains two fesv-specific antigens (p15 and p12), and the feline oncornavirus-associated cell membrane antigen (focma). all three antigens are helper virus-independent and are encoded by the fesv genome, focma, p15, and p12 antigens cochromatograph as phosphorylated molecules of 85,000 molecular weight (pp85), adsorb to immunoadsorbant columns prepared with antibodies to feline leukemia virus (felv), and are prec ... | 1978 | 206902 |
| production of antibodies against feline leukemia and sarcoma viruses and the feline oncovirus-associated cell membrane antigen in strain 2 guinea pigs. | 1978 | 210573 | |
| characterization of feline glomerulonephritis associated with viral-induced hematopoietic neoplasms. | light, electron, and immunofluorescence microscopy on tissues from 63 domestic cats revealed that glomerulonephritis occurred in almost one third of cats with hematopoietic neoplasms of the type linked with feline leukemia virus (felv). glomerular lesions were of the immune complex type with subepithelial, subendothelial, and mesangial dense deposits and reticular aggregates, similar to the nephropathy associated with systemic lupus erythematosus in humans. evidence that the glomerular lesions m ... | 1978 | 677265 |
| [feline leukemia: recent developments on the causes and immunoprevention of the disease (author's transl)]. | 1978 | 230008 | |
| modifications of the immunofluorescence assay for feline leukemia virus group-specific antigens. | observations and minor modifications are presented concerning the immunofluorescence assay for feline leukemia virus (felv) group-specific antigens (gsa) in blood cells of cats. data are given regarding absorption of goat felv gsa antiserum in vivo in cats, absorption of the antiserum in vitro with feline blood cells, and the comparative efficacy of various chemical fixatives in preservation of felv gsa for immunofluorescent staining. the best results were obtained with in vitro absorption of an ... | 1978 | 219732 |
| influence of thymectomy on the susceptibility of cats to feline leukemia virus and lymphosarcoma. | twelve cats were thymectomized at 5 weeks of age. six of these cats were inoculated at 8 weeks of age and 6 at 4 months of age with the rickard (r) strain of feline leukemia virus (felv), which produces a high incidence of thymic lymphosarcoma. two groups of age-matched nonthymectomized cats were inoculated with the same felv-r stock. thymectomy prior to felv infection had no influence on the induction of viremia or the incidence of lymphosarcoma. in the felv-inoculated nonthymectomized cats, ly ... | 1978 | 208436 |
| endogenous feline (rd-114) and baboon type c viruses have related specific rna-binding proteins and genome binding sites. | 1978 | 202083 | |
| deposition of retrovirus associated antigens (p30 and gp70) on cell membranes of feline and murine leukaemia virus infected cells. | a quantitative estimation of retrovirus associated cell membrane antigens of murine and feline cells infected with their respective type c leukosis virus is presented. using a radio-immune assay with three broadly reactive antisera, the minimum estimated number of retrovirus associated antigenic determinants on yac [moloney leukaemia virus (mulv) infected murine] and fl-74 [feline leukaemia virus (felv) infected feline] cells was 1.3 x 10(6) and 1.6 x10(6) determinants per cell respectively. the ... | 1978 | 75945 |
| experimental horizontal transmission of feline leukemia viruses of subgroups a, b, and c. | experiments were conducted with cats in metal cages inside plastic isolators to determine whether feline leukemia virus (felv) of the three known antigenic subgroups (a, b, and c) is transmitted horizontally from infected cats to normal "contact" cats. blood smears collected at weekly intervals were examined by the fixed-cell, indirect immunofluorescence test for felv internal p30 to follow the spread of infection from inoculated cat to contact cats. these studies established that viruses of all ... | 1978 | 75981 |
| endogenous mink (mustela vison) type c virus isolated from sarcoma virus-transformed mink cells. | a previously described type virus stock (designated pp-1r), isolated by cocultivating baboon cells with mink cells transformed by kirsten sarcoma virus (64j1), has been further cloned and characterized. end point-diluted stocks of pp-1r have been obtained that are free of focus-forming activity and lack both kirsten sarcoma and primate type c viral sequences. nucleic acid hybridization experiments show that the cloned virus (milv) is an endogenous, genetically transmitted virus of the mink (mus ... | 1978 | 76684 |
| rd-114 virus: characterization and identification. | 1978 | 77028 | |
| multiple rnase h activities in mammalian type c retravirus lysates. | lysates of moloney murine sarcoma-leukemia virus [m-msv(mlv)], a virus complex grown in the rat cell line 78a-1, were found to contain three rnase h species separable by polycytidylic acid[poly(c)]-agarose chromatography. rnase h activity (rnase h i) associated with rna-directed dna polymerase eluted at 0.23 m kci from poly(c)-agarose. rnase h ii, which eluted from poly(c)-agarose at 0.12 m kci and was not associated with dna polymerase activity, was shown to be identical to an rnase h species ( ... | 1978 | 77333 |
| the biosynthesis of oncovirus proteins. | the patterns of oncovirus protein biosynthesis are essentially similar for avian and mammalian viruses. in each case the four major internal structural proteins are synthesized as a precursor polypeptide of about 75 000 daltons, the product of the gag gene. translation occurs on genome-sized mrna. this polyprotein is cleaved in a series of steps to give the mature proteins. the mechanism and localization of cleavage have not yet been clarified. viral reverse transcriptase, the product of the pol ... | 1978 | 77678 |
| surface antibodies of human myelogenous leukaemia leukocytes reactive with specific type-c viral reverse transcriptases. | purified immunoglobin g (igg) from patients with chronic myelogenous leukaemia specifically neutralised rt from feline leukaemia virus while purified igg from other types of leukaemias and from normal blood cells were less reactive and in some cases preferentially reacted with rt from horizontally transmitted primate type-c viruses (simian sarcoma virus-gibbon ape leukaemia virus group). this indicates the presence of a heterogeneous immune response to rt or to an rt-like molecule in humans. | 1978 | 82207 |
| antibody response in cats to feline leukemia virus reverse transcriptase under natural conditions of exposure to the virus. | 1978 | 84436 | |
| radioimmunologic characterization of rd-114 reverse transcriptase: evolutionary relatedness of mammalian type c viral pol gene products. | 1978 | 78564 | |
| [immunotherapy of malignant growths (author's transl)]. | our knowledge of antigens which are associated with different types of malignant tumours is steadily increasing. these antigens exist in considerable numbers, but, so far with few exceptions, only their presence can be demonstrated by certain methods; to isolate and identify them has not yet been possible. these antigens are, therefore, suitable not so much for the primary diagnosis, but rather, like the carcinoembryonic antigen, the tissue-polypeptide antigen or the alpha-feto-protein, for asse ... | 1979 | 88728 |
| sarcoma virus-induced transformation specific antigen: presence of antibodies in cats that were naturally exposed to leukemia virus. | 1979 | 88797 | |
| characterization of reverse transcriptase from filine leukemia virus by radioimmunoassay. | 1979 | 91258 |