Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| h-2 compatability requirement for t-cell-mediated lysis of target cells infected with lymphocytic choriomeningitis virus. different cytotoxic t-cell specificities are associated with structures coded for in h-2k or h-2d;. | use of syngeneic, allogeneic, f1, and h-2 recombinatn mice has shown that animals injected with lymphocytic choriomeningitis (lcm) virus generate t cells which are cytotoxic for h-2k or h-2d compatible, but not h-2 different, virus-infected target cells. three separate lines of evidence are presented which indicate that these immune t cells are sensitized to "altered-self," the self antigens involved being coded for in the h-2k or h-2d regions. firstly, cytotoxic activity associated with mutuali ... | 1975 | 47901 |
| letter: lymphocytic choriomeningitis associated with hamsters. | 1975 | 48074 | |
| peritoneal macrophages as target cells for measuring virus-specific t cell mediated cytotoxicity in vitro. | in inbred or outbred mice lysis of virally modified cells by thymus-derived lymphocytes occurs only within a h-2 compatible system. peritoneal macrophages provide a convenient source of syngeneic target cells. they are readily infected in vitro with lcm virus, and show high levels of specific 51cr release when exposed to t cells from lcm immune mice. | 1975 | 52680 |
| a comparison of biochemical and biological properties of standard and defective lymphocytic choriomeningitis virus. | lymphocytic choriomeningitis (lcm) virus infection of the mouse is the best-studied model of persistent viral infection. in cell culture, persistent lcm virus infections are associated with the production of large quantities of defective interfering (di) lcm virus. these defective interfering particles cannot replicate by themselves yet can interfere with the replication of the standard virus and prevent the cytolytic effect caused by the standard virus. it is important to determine the mechanis ... | 1975 | 60182 |
| h-2 compatibility is required for t-cell-mediated lysis of target cells infected with lymphocytic choriomeningitis virus. | maximal cell-mediated lysis of targets infected with lymphocytic choriomeningitis virus occurs only within a h-2 compatible system. syngeneic immune spleen cells are at least 100 times as effective as are allogeneic lymphocytes. reciprocal restriction of cytotoxic t-cell activity has been shown to operative between h-2k, h-2d, and h-2b. experiments with cogenic mice have localized the effect to the h-2 gene complex. furthermore, the observation that lymphocytes from h-2a mice cause high specific ... | 1975 | 123002 |
| cytotoxic t cell activity is strain-specific in outbred mice infected with lymphocytic choriomeningitis virus. | the cytotoxic t cell response in outbred mice infected with lymphocytic choriomeningitis virus (lcmv) is strain specific. the same is true for adoptive transfer of fatal lcm disease. the response of individuals within an outbred strain is completely cross-reactive, as shown by using immune lymphocytes and virus-infected macrophage targets from individual mice. reciprocal exclusion of cytotoxic t cell activity between inbred and outbred mouse strains is the rule, the exception being one strain (h ... | 1975 | 127010 |
| arenavirus taxonomy: a review. | despite a late beginning, the construction of the arenavirus taxon and its placement in the scheme of the international committee on taxonomy of viruses has now been completed. the bringing together of the member viruses has already provided valuable indications of promising laboratory and field study approaches; in the future this classification will contribute further to our understanding of the natural history and disease processes of the human pathogens of the group. | 1975 | 182395 |
| morphology and morphogenesis of arenaviruses. | arenaviruses have unique structural characteristics; they are pleomorphic, have a mean diameter of 110-130 nm, and consist of a membranous envelope with surface projections surrounding an interior containing ribosomes and filaments. virus particles bud from plasma membranes of infected cells and in many cases large intracytoplasmic inclusion bodies are formed. these characteristics allow generic identification, but not differentiation of individual viruses. ultrastructural identification of viru ... | 1975 | 182396 |
| antigenic properties of the arenaviruses. | arenaviruses are known to show antigenic relationships in the complement-fixation (cf) and fluorescent antibody tests but not in the neutralization test. the humoral response to some of the arenaviruses is characterized by a dissociation between the antibodies determined by the cf test and those determined by the neutralization test and also by its late appearance following natural infection. investigations are reported showing that, as regards the cf test, junin, machupo, amapari, and tacaribe ... | 1975 | 182397 |
| antigenic relationships of lymphocytic choriomeningitis virus and tacaribe virus in the indirect haemagglutination test. | the indirect haemagglutination test has been developed for use with lcm and tacaribe viruses employing antibody-sensitized erythrocytes. both in this test and in the indirect haemagglutination inhibition test cross-reactions between lcm and tacaribe viruses were revealed. this opens new perspectives for group and species identification of arenaviruses. | 1975 | 182398 |
| the structure of rodent faunas associated with arenaviral infections. | the biogeographical examination of rodent faunas associated with arenaviruses reveals two distinct patterns. lymphocytic choriomeningitis (lcm) virus is associated primarily with a single murid species, mus musculus, although it is also known to cause laboratory infections in other species. on the other hand, the arenaviruses from the western hemisphere are associated exclusively with a large and diverse group of cricetid rodents. studies to date, although limited, have not demonstrated their as ... | 1975 | 182403 |
| status of arenavirus vaccines and their application. | a limited but definite need exists for vaccines against lassa, junin, and machupo viruses. medical and laboratory personnel, as well as defined high-risk population groups, require protection from these highly virulent agents. to date little work has been done on inactivated vaccines for these viruses. a live attenuated junin vaccine has been tested successfully in more than 600 persons, and a high-passage machupo virus strain has protected rhesus monkeys against lethal infection produced by a h ... | 1975 | 182407 |
| virus neutralization and virus-induced immune complex disease. virus-antibody union resulting in immunoprotection or immunologic injury--two sides of the same coin. | 1975 | 164055 | |
| arenaviruses. | 1975 | 168692 | |
| pathogenic mechanisms of tissue injury in persistent viral infections. | 1975 | 169724 | |
| effects of viruses on immune response. | 1975 | 171931 | |
| use of iodinated organic compounds for the density gradient centrifugation of viruses. | use of urografin and conray for the equilibrium centrifugation of viruses is described. these pharmaceuticals, which consist of iodinated arylic compounds, reach densities of 1.6 g/cm3 and have low intrinsic viscosities. poliovirus, newcastle disease virus, and lymphocytic choriomeningitis virus were centrifuged to equilibrium in gradients made of these substances. viral infectivities were not measurably affected, which is especially noteworthy in the case of the very labile lymphocytic choriome ... | 1975 | 174530 |
| t lymphocyte function as the principal target of lymphocytic choriomeningitis virus-induced immunosuppression. | plaque-forming cell responses against sheep erythrocytes, escherichia coli lipopolysaccharide, pneumococcal polysaccharide, and polyvinylpyrrolidone were examined in mice infected with lymphocytic choriomeningitis virus. a 92 to 96 percent reduction of the thymus-dependent anti-sheep erythrocyte responses was observed 2 to 4 weeks after infection. however, the thymus-independent responses against the three other antigens were close to normal at all stages of the infetion. studies on allograft ... | 1975 | 235488 |
| antibody to lymphocytic choriomeningitis virus in children with congenital hydrocephalus. | antibody to lymphocytic choriomeningitis (lcm) virus was found by the indirect immunofluorescence (if) technique in high titres in 9 out of 28 children with congenital hydrocephalus and in moderate titres in 6 mothers of these seropositive children. it is suggested that lcm virus or a closely related virus infecting the foetus in utero may play a role in the aetiopathogenesis of some cases of congenital hydrocephalus. | 1975 | 239565 |
| microplaque reduction: new assay for neutralizing antibody to lymphocytic choriomeningitis virus. | a plaque-reduction neutralization test for lymphocytic choriomeningitis virus was developed; microtiter dilution techniques and assay plates were used for the new test. plaques develop by day 4 after inoculation of serum and lymphocytic chorio-meningitis virus into 5-mm cups containing agarose suspension of baby hamster kidney cells. the method lends itself well to the titration of neutralizing antibody and gives rapid results with great economy of reagents. when tested with one batch of 55 lymp ... | 1975 | 1090687 |
| lymphocytic choriomeningitis virus in man. serologic evidence of association with pet hamsters;. | laboratory evidence of recent or current lymphocytic choriomeningitis (lcm) virus infection was obtained in 60 patients. twelve had diagnosis of central nervous system (cns) infection: four of meningoencephalitis and eight of meningitis. thirty-four patients had a grippe-like syndrome. fifty-nine had had contact with pet hamsters. all of the 24 patients whose pets were studied had been exposed to one or more hamsters with serologic evidence of past lcm virus infection. the data implicate pet ham ... | 1975 | 1091750 |
| outbreak of lymphocytic choriomeningitis virus infections in medical center personnel. | an outbreak of lymphocytic choriomeningitis (lcm) virus infections occurred in medical center personnel at the university of rochester in 1972-1973. a total of 48 infections was discovered, 31 in staff of the radiation therapy area, 12 in the staff of the vivarium facility, and 5 in other individuals who worked in the medical center. twenty-one of the infections were associated with recent febrile illness with myalgia. epidemiologic and virologic studies indicated that the source of the infectio ... | 1975 | 1092154 |
| [titration of antibodies to lymphocytic choriomeningitis virus by the method of indirect immunofluorescence]. | antibody to lymphocytic choriomeningitis virus was determined by the indirect immunofluorescence test in immune sera of guinea pigs and immune ascitic fluids of rats and mice. among 135 patients with aseptic meningitis serum antibody was found in 11 patients in titers of 1 : 64 to 1 : 128 and in the cerebro-spinal fluid of these patients in considerably lower titers. by the indirect immunofluorescence test antibody in maximum titers was found early after the appearance of meningeal symptoms. | 1975 | 1099805 |
| comparison of three tests for the serological diagnosis of lymphocytic choriomeningitis virus infection. | levels of lymphocytic choriomeningitis virus antibody were assayed in 62 infected persons. the three tests used were indirect fluorescent antibody (ifa), complement fixation, and neutralization in mice. the sera first became positive by the ifa test, and ifa titers rapidly rose to a relatively high level, with the sera remaining positive long after the antibody detectable by complement fixation had disappeared. the ifa test appeared to be specific. the sera became positive last by the mouse neut ... | 1975 | 1100673 |
| pathogenesis of cerebellar hypoplasia produced by lymphocytic choriomeningitis virus infection of neonatal rats. ii. an ultrastructural study of the immune-mediated pathology. | lymphocytic choriomeningitis virus, following inoculation of 4-day-old rats, produces an acute destructive but nonfatal necrosis of the cerebellum which is immunologically mediated. an ultrastructural study of the developing lesion indicated the following sequence of events: first, lymphocytic choriomengitis virions appear in the intercellular spaces of apparently normal neural parenchyma, followed shortly thereafter (5 days after infection) by adherence of monocytes to vascular endothelium with ... | 1975 | 1104999 |
| course of lymphocytic choriomeningitis virus infection in young and aged mice. | the course and histological picture of intracerebral lcm virus infection was found to differ in mice with their thymus displaying an age-dependent involution and in young adult animals. signs of lymphocytic choriomeningitis were absent in 75 per cent of the aged animals. the change of the characteristic clinical and histological picture referred to the decreased cellular immune response of aged animals, ascribed to the age-dependent involution of the thymus. | 1975 | 1111261 |
| lymphocytic choriomeningitis in university hospital personnel. clinical features. | clinical manifestations of lymphocytic choriomeningitis (lcm) virus infection in 15 patients are described. these patients were university hospital personnel who had had contact with hamsters, subsequently shown to harbor the virus. fever with striking myalgias, headache and rigors were the most common symptoms. only 2 of the 15 patients had clinically overt and documented aseptic meningitis. leuikpenia was observed in 10 of 11 patients and thrombocytopenia in 8 of 8 patients tested. a biphasic ... | 1975 | 1138538 |
| meningoencephalitis with toroidal virus-like particles. | a previously healthy middle aged man died following a 6 month illness which presented with middle ear symptoms, apparently resolved, and then 2 months later manifested as encephalitis. the illness was characterized initially by depression and intellectual deterioration. no family member or working associate was affected. the clinical diagnosis of viral encephalitis was confirmed by brain biopsy but no virus was isolated in the laboratory. numerous intracisternal toroidal virus-like particles wer ... | 1975 | 1155039 |
| combined phytohaemagglutinin and dianhydrodulcitol treatment of lymphocytic choriomeningitis virus infection in mice. | the course of intracerebral lymphocytic choriomeningitis (lcm) virus infection was studied in mice treated simultaneously with dianhydrodulcitol (dad) and phytohaemagglutinin (pha). earlier experiments revealed that dad decreased and pha enhanced the cellular immune response of mice to lcm virus infection. on applying the treatments simultaneously they inhibited each other and neither the decreasing effect of dad nor the enhancing effect of pha on the cellular immune response could be observed. | 1975 | 1155228 |
| laboratory studies of a lymphocytic choriomeningitis virus outbreak in man and laboratory animals. | investigation of an outbreak of prolonged febrile illness in medical center personnel at the university of rochester school of medicine and dentistry revealed lymphocytic choriomeningitis (lcm) virus to be the causative agent. syrian or golden hamsters (mesocricetus auratus) were found to be the only animals involved in maintaining the virus and were the source of human infections. isolations of lcm virus were made from autopsy specimens of 13 of 46 (28%) golden hamsters. virus isolations were m ... | 1975 | 1163529 |
| micromethod for the titration of lymphocytic choriomeningitis virus in cell cultures. | a quantal microassay for the titration of lcm virus strains is described. it is based on the detection of virus-specific complement-fixing antigen in the medium of infected l cell microcultures. | 1975 | 1167575 |
| lymphocytic choriomeningitis virus. i. concentration and purification of the infectious virus. | two procedures for the purification of infectious lymphocytic choriomeningitis virus from cell culture fluid have been developed. if large quantities of very pure virus are to be prepared, infected l cells are maintained with a medium supplemented with calf serum, the proteins of which have been largely removed by pretreatment with polyethylene glycol. two days after infection of the cultures, the media are collected and the virus is concentrated by treatment with polyethylene glycol 40,000. pur ... | 1975 | 1170337 |
| the treatment of 18 cases of malignant disease with an arenavirus. | 1975 | 1183104 | |
| lymphocytic choriomeningitis: ultrastructural pathology. | 1975 | 1183539 | |
| transient impaired cell-mediated tumor immunity after acute infection with lymphocytic choriomeningitis virus. | intraperitoneal injection of nononcogenic lymphocytic choriomeningitis (lcm) virus in adult c3h mice causes a symptomless infection but stimulates specific cell-mediated and humoral immune responses. however, median survival time of virus-infected mice inoculated with syngeneic tumor cells was significantly shortened, and growth of semiallogeneic tumors was significantly enhanced. cell-mediated cytotoxicity measured as chromium-51 release from labeled tumor cells was significantly suppressed but ... | 1975 | 1188321 |
| observations on "late onset disease" and tumor incidence in different strains of laboratory mice infected congenitally with lcm virus. i. experiments with random-bred nmri mice. | 1975 | 1199541 | |
| observations on "late onset disease" and tumor incidence in different strains of laboratory mice infected congenitally with lcm virus. ii. experiments with inbred cba/j mice. | 1975 | 1199542 | |
| [study of viremia in experimental neuroviral infections]. | study of viremia in susceptible experimental animals showed all the blood fractions to take part in the infectious process both in acute diseases and in the chronic course of infections caused by various neuroviruses. thus in animals infected with human acute encephalomyelitis, tick-borne encephalitis, lymphocytic choriomeningitis viruses viremia was observed throughout the entire short period of infection terminating fatally, as a rule. in such cases viremia was of a mixed character, the viruse ... | 1975 | 1220245 |
| [study of arenaviruses in the indirect hemagglutination reaction]. | the indirect hemagglutination test with erythrocytes sensitized with antibody to lcm and tacaribe viruses, most typical members of of arenaviruses, has been developed. this test is a sensitive method for detection of arenaviruses in infected materials in vitro. the indirect hemagglutination test and the indirect hemagglutination-inhibition test revealed cross-interaction between lcm and tacaribe viruses which opens new approaches for group and species identification of arenaviruses. | 1975 | 1226709 |
| studies on the consequences of lymphocytic choriomeningitis virus infection in phytohaemagglutinin-treated mice. | 1975 | 1235956 | |
| [detection of persistent infection of stable cell culture with an agent related to lymphocytic choriomeningitis virus]. | the presence of a persisting agent related to lymphocytic choriomeningitis virus has been detected in a number of stable cell cultures of human and animal origin by means of the indirect immunofluorescence procedure. | 1975 | 769341 |
| role of substrains in persistent lymphocytic choriomeningitis virus infection. | two plaque-type mutants, a wild strain mb(1), and a slow-growing non-plaque-forming variant of lcm virus were compared in l cell cultures. the cultures were monitored at biweekly intervals following infection for plaque-forming virus titre and cytoplasmic and membrane specific immunofluorescence of the cells. different patterns were obtained in each case and the relevance of these results to lcm persistence is discussed. while the lm variant continually produced virus for 35 transfers, with a cy ... | 1975 | 782736 |
| arenavirus inactivation on contact with n-substituted isatin beta-thiosemicarbazones and certain cations. | n-methyl and n-ethyl isatin beta-thiosemicarbazones inactivate cell-free parana and pichinde viruses as well as three strains of lymphocytic choriomeningitis virus. this antiviral activity is abolished in the presence of the chelating agent edta. the rate of virus inactivation by n-methyl isatin beta-thiosemicarbazone is greatly enhanced and controlled by the addition of cupric sulphate. divalent cations of other first transition series metals are less effective. a difference exists in the coppe ... | 1975 | 809544 |
| editorial: viral haemorrhagic fevers. | 1975 | 810212 | |
| studies of abluminuria and proteinuria in normal mice and mice with immune complex glomerulonephritis. | proteinuria is supposedly a frequent and early manifestation of glomerulonephritis. since albuminuria rather than proteinuria is the hallmark of glomerular disease, the present studies were designed to study the occurrence of albuminuria in normal mice (swr/j strain) and in mice with a reproducible and predictable immune complex glomerulonephritis induced by chronic infection with lymphocytic choriomeningitis (lcm) virus. a radial immunodiffusion technique, specific for mouse albumin, was employ ... | 1975 | 811741 |
| [virological control of laboratory mice]. | virological control of laboratory mice can be obtained by serology or virus isolation from the animals. advantages and inconveniencies of these two techniques are discussed. special techniques i.e. preparation of immune sera or cultures of infected tissues, are also described. | 1975 | 821324 |
| capacity of sensitized thymus-derived lymphocytes to induce fatal lymphocytic choriomeningitis is restricted by the h-2 gene complex. | adoptive immunization of syngeneic, immunosuppressed recipients infected with lymphocytic choriomeningitis (lcm) virus causes fatal neurologic disease within 2 to 4 days of cell transfer, providing that donors are sampled when the in vitro 51-cr release assay shows maximal specific activity of sensitized thymus-derived lymphocytes (t cells). prior treatment of immune spleen cells with akr anti-omicron ascitic fluid and complement causes total abrogation of this in vivo activity. fatal neurologic ... | 1975 | 1078677 |
| genes required for cytotoxicity against virus-infected target cells in k and d regions of h-2 complex. | 1975 | 1078719 | |
| enhanced immunological surveillance in mice heterozygous at the h-2 gene complex. | 1975 | 1079575 | |
| the activity of t and b lymphocytes in immunity and tolerance to the lymphocytic choriomeningitis virus in mice. | treatment with anti-theta serum and the wigzell column technique for cell separation was employed to study the separate functions of the b and t lymphocytes in the late states of immunity to the lcm virus in mice. the cell preparations examined were mixtures of spleen and lymph node cells from immune mice. the results revealed that the anti-viral effect of such cells after transfer to virus carriers was unimpaired in t cell-enriched and b cell-deprived cell preparations. the anti-viral effect wa ... | 1975 | 1080743 |
| temporary presence of self-reactive cytotoxic t lymphocytes during murine lymphocytic choriomeningitis. | 1975 | 1081650 | |
| lcm virus infection of cells in vitro. | most mammalian cells cultivated in vitro can be infected with lymphocytic choriomeningitis (lcm) virus. in addition to infectious virus, the cells produce antigenic material that fixes complement in the presence of antibody and is precipitated by antiserum. intracellular antigen can also be demonstrated by the immunofluorescence procedure. when infected cells are viewed with the electron microscope, viral structures are seen either budding from or in association with the cell membranes. immunoel ... | 1975 | 1085205 |
| immune responses to lcm virus infection in vivo and in vitro. mechanisms of immune-mediated disease. | both recovery and death of mice following acute infections with lymphocytic choriomeningitis virus appear to be mediated by a population of virus-specific thymus-derived (t) effector lymphocytes that possess lytic activity in vitro against virus-infected syngeneic fibroblasts. whether recovery or death occurs is determined by the balance between two interdependent factors: (1) the extent of virus-induced modifications in the surfaces of cells comprising "target" tissues, and (2) the efficiency o ... | 1975 | 1085206 |
| some observations on hamster-derived human infection with lymphocytic choriomeningitis virus. | the sequence of events leading to the diagnosis of recent outbreaks of hamster-borne lymphocytic choriomeningitis (lcm) in new york state is briefly reviewed. some relevant experiments on persistent lcm infection of hamsters are described, including measurements of complement levels in the affected animals. evidence is discussed which suggests that complement has an important role in eliminating free virus in the circulation by participating in virus neutralization. | 1975 | 1085211 |
| secondary cytotoxic cell response to lymphocytic choriomeningitis virus ii. nature and specificity of effector cells. | the method described in the previous paper was used to induce secondary responses in spleen cells from cba/h mice, pre-primed with lymphocytic choriomeningitis (lcm) virus by culturing them with lcm-infected peritoneal cells. the cytolytic effector cells thus generated have been characterized. effector cells were sensitive to treatment with anti-theta ascitic fluid and complement. separation procedures based on rosetting of certain categories of lymphocytes with sheep red cells through an isopaq ... | 1976 | 1085282 |
| h-2 compatibility requirement for virus-specific t-cell-mediated cytolysis. evaluation of the role of h-2i region and non-h-2 genes in regulating immune response. | lymphocytic choriomeningitis virus (lcmv) and ectromelia virus-specific t-cell-mediated cytotoxicity was assayed in various strain combinations using as targets peritoneal macrophages which have been shown to express ia antigens. virus-specific cytotoxicity was found only in h-2k- or d-region compatible combinations. i-region compatibility was not necessary nor alone sufficient for lysis. six different i-region specificities had no obvious effect on the capacity to generate in vivo specific cyto ... | 1976 | 1085331 |
| h-2 restriction of virus-specific t-cell-mediated effector functions in vivo. ii. adoptive transfer of delayed-type hypersensitivity to murine lymphocytic choriomeningits virus is restriced by the k and d region of h-2. | in mice, primary footpad swelling after local infection with lymphocytic choriomeningitis virus (lcmv) and delayed-type hypersensitivity (dth) adoptively transferred by lcmv immune lymphocytes are t-cell dependent. nude mice do not develop primary footpad swelling, and t-cell depletion abrogates the capacity to transfer lcmv-specific dth. effector t cells involved in eliciting dose-dependent dth are virus specific in that vaccinia virus-immune lymphocytes could not elicit dth in lcmv-infected mi ... | 1976 | 1085340 |
| cells that express viral antigens but lack h-2 determinants are not lysed by immune thymus-derived lymphocytes but are lysed by other antiviral immune attack mechanisms. | murine f9 teratoma cells do not express major transplantation antigens detectable by either serologic or alloreactive assays of thymus-dependent lymphocytes (t cells). such cells can be infected with lymphocytic choriomeningitis virus or vaccinia virus, do express viral antigens on the cell surface, and can release infectious virus in amounts equivalent to those of other h-2 bearing murine cell lines. immunologic injury of virus-infected f9 cells occurs after the addition of specific antiviral a ... | 1976 | 1086476 |
| the cytotoxicity of specifically sensitized lymphocytes from mouse strains of varying h-2 specificities on lcm virus-infected l cells. | during an lcm virus infection in mice, cytotoxic t lymphocytes appear to be directed against target cells infected with the virus. this cytotoxic reaction is restricted by the h-2 histo-compatibility between effector and target cells. to analyse this restriction, the cytotoxic effect of in vivo sensitized spleen cells from mice of various h-2 specificities on lcm virus-infecte of in vivo sensitized spleen cells from mice of various h-2 specificities on lcm virus-infected l cells (h-2k) was studi ... | 1976 | 1087102 |
| changes in the surface of virus-infected cells recognized by cytotoxic t cells. ii. a requirement for glycoprotein synthesis in virus-infected target cells. | infection of cells with either ectromelia or lymphocytic choriomeningitis (lcm) virus in the presence of 2-deoxy-d-glucose (2-dog) inhibited by up to 70% the extent to which the infected cells become susceptible to virus-specific cell-mediated lysis. the concentration of 2-dog used had little effect on the extent of total protein synthesis (incorporation of [35s] methionine) but inhibited (up to 25%) glycoprotein synthesis, as measured by incorporation of [3h] fucose. this suggested that glycopr ... | 1976 | 1087455 |
| h-2 compatibility requirement for virus-specific t-cell-mediated cytolysis. the h-2k structure involved is coded by a single cistron defined by h-2kb mutant mice. | lymphocytic choriomeningitis or vaccinia virus-immune spleen cells of h-2 mutant mice carrying a point mutation in the k region (b6 h-2ba, b6 h-2bf) cannot lyse infected wild-type h-2kb targets and vice versa. yet, cytotoxic t cells specific for infected h-2kba or h-2kbf targets are generated during virus infections as shown by cold target competition experiments. the critical structure for the apparent restriction by the k or d regions of the h-2 gene complex of cytolytic interactions between t ... | 1976 | 1082490 |
| lymphocytic choriomeningitis virus-induced ocular immunopathology. | lymphocytic choriomeningitis (lcm) virus of the mice provides perhaps the most extensively studied model of viral-induced immunopathology. intraocular inoculation of this virus in the adult mouse produces a severe and progressive uveitis with involvement also of the cornea and retina. intracerebral infection of the newborn rat with lcm virus produces a severe retinopathy, with only minimal involvement of the uveal tract. we review in this paper recent studies on these two animal models of lcm vi ... | 1976 | 1082541 |
| virus and trinitrophenol hapten-specific t-cell-mediated cytotoxicity against h-2 incompatible target cells. | immune spleen cells from lcm virus-infected (cba x c57bl/6)f1 radiation chimeras entirely repopulated with cba-t6 lymphocytes were cytotoxic for allogeneic, lcm virus infected c57bl/6 mouse-derived target cells. normal c57bl/6 targets were not lysed. cba-t6 lymphocytes derived from (cba x c57bl/6) radiation chimeras sensitized in vitro against tnp-conjugated c57bl/6 spleen cells lysed tnp-conjugated c57bl/6 targets. however normal c57bl/6 mouse-derived targets were not destroyed. the magnitude o ... | 1976 | 1082923 |
| virus-specific t-cell-mediated cytotoxicity across the h-2 barrier to virus-altered alloantigen. | 1976 | 1083955 | |
| [acute and chronic infection of mice, caused by lymphocytic choriomeningitis virus isolated from mus musculus in moscow]. | infection of mice with strain no. 325 of lymphocytic choriomeningitis virus (lcm) isolated in moscow was of two types depending on the age of the animals at the time of inoculation. infection in adult mice terminated either in death or recovery with development of immunity. inoculation of suckling mice resulted in formation of chronic virus carrier state in most survivors. the indirect hemagglutination inhibition test was found to be very sensitive for detection of antibody in convalescent anima ... | 1976 | 936582 |
| [role of lymphocytic choriomeningitis virus in the etiology of congenital hydrocephalus]. | by the indirect immunofluorescence test, antibodies to lymphocytic choriomeningitis virus in titers of 1:32 to 1:512 detected in 12 out of 32 infants under 1 with congenital hydrocephalus. the majority of the mothers of these infants were also shown to possess these antibodies in low and moderate titers. the assumption that lymphocytic choriomeningitis virus infecting the fetus in utero may cause the development of hydrocephalus is substantiated. in 11 of 12 seropositive infants foci of choriore ... | 1976 | 943891 |
| secondary cytotoxic cell response to lymphocytic choriomeningitis virus. i. kinetics of induction in vitro and yields of effector cells. | secondary (memory) cell-mediated cytotoxic responses in lymphoid cells from cba/h mice pre-primed with lymphocytic choriomeningitis virus (lcm) 5-7 weeks previously were induced by culturing these cells in vitro with syngeneic, infected peritoneal cells at 37 degrees for periods of up to 5 days. cytotoxic effectors were assayed against lcm infected, h-2 compatible target cells in a 51cr release assay. response was greater with a higher ratio (1:10) of infected peritoneal cells:pre-primed cells t ... | 1976 | 955675 |
| lymphocytic choriomeningitis virus-induced immunosuppression: a virus-induced macrophage defect. | primary immunizing infections with lcm virus result in a transient depression of the in vitro proliferative responses of splenic lymphocytes to mitogens specifically reactive with t cells or b cells. this depression of lymphocyte function is the result of a virus-induced defect in an adherent, phagocytic cell population required for in vitro lymphocyte activation. depressed responses persist for about 1 week after virus clearance and can be corrected by the addition of normal pem or 2-me to infe ... | 1976 | 956645 |
| the ability of athymic nude mice to survive intracerebral infection of lymphocytic choriomeningitis (lcm) virus. | adult athymic nude mice were protected against the lethality of intracerebral infection with lcm virus. although the multiplication of lcm virus in the brain was almost equal between nu/nu and nu/+ mice, the delayed hypersensitivity reaction was not evoked in nu/nu mice as evidenced by foot pad reaction. | 1976 | 966396 |
| [main syndrome of prenatal infection caused by lymphocytic choriomeningitis virus - hydrocephalus and chorioretinal degeneration]. | 1976 | 972829 | |
| homologous interference of lymphocytic choriomeningitis virus: detection and measurement of interference focus-forming units. | lymphocytic choriomeninigitis (lcm) virus defective interfering (di) particles form foci of protected cells in a monolayer under an agarose-containing overlay medium. foci originate from one cell dually infected with at least 1 interference focus-forming unit and infectious virus. as a result, an interfering factor is produced and released which interacts with neighboring cells, thereby protecting them against cytopathic lysis by challenge virus. the property of individual lcm virus di particles ... | 1976 | 978787 |
| characterization of ribonucleoproteins and ribosomes isolated from lymphocytic choriomeningitis virus. | disruption of purified lymphocytic choriomeningitis (lcm) virus with nonidet p-40 in 0.5 m kcl followed by sucrose gradient centrifugation in 0.3 m kcl led to the isolation of two viral nucleoproteins (rnps) as well as 40s and 60s ribosomal subunits. the largest viral rnp sedimented heterogenously at 123s to 148s and was associated with 23s and 31s viral rna. the other viral rnp sedimented at 83s and was associated with 23s viral rna. the buoyant density in cscl was determined to be 1.32 g/cm3 f ... | 1976 | 978789 |
| lymphocytic choriomeningitis from pet hamster exposure: a local public health experience. | eleven cases of hamster associated lymphocytic choriomeningitis occurred within a space of four months in jefferson county, alabama residents. a single birmingham breeder was the source of the infected hamsters. ten cases experienced an illness of one to three weeks duration with grippe-like symptoms being most frequent. complaints of nine cases suggested meningeal involvement, and one was asymptomatic. all patients recovered uneventfully. frequent hamster contact had occurred in most cases, alt ... | 1976 | 984278 |
| antibody-complement interactions with purified lymphocytic choriomeningitis virus. | 1976 | 986722 | |
| analysis of baby hamster kidney cells persistently infected with lymphocytic choriomeningitis virus. | baby hamster kidney cells were persistently infected with lymphocytic choriomeningitis (lcm) virus (bhkpi cells). after 21 passages of the bhkpi cells infectious virus could no longer be detected; however, the cultures continued to produce lcm virus particles which interfered with the replication of infectious lcm virus in bhkpi cells and protected mice from a subsequent intracranial inoculation of infectious lcm virus. cultures of bhkpi cells appeared to consist of three cell populations: uninf ... | 1976 | 987157 |
| [choriomeningitis virus as etiologic agent of neuroinfections in poland]. | 1976 | 1013366 | |
| hydrocephalus due to prenatal infection with the lymphocytic choriomeningitis virus. | the results are presented of serological examinations in a total of 4235 subjects including blood donors (341 persons), pregnant women (1784), newborns (833), patients with malignant tumours (248), patients with influenza-like diseases (548), patients with abacterial meningitis (295), infants under one year with hydrocephalus (40), infants under two years with other nervous system diseases (110), mothers of seropositive children with hydrocephalus (12) and mothers of seronegative children with h ... | 1976 | 1017876 |
| familial amyotrophic lateral sclerosis. | 1976 | 1028253 | |
| the hamster as a secondary reservoir host of lymphocytic choriomeningitis virus. | exposure of weaned hamsters to an environment contaminated with lcm virus shed by tolerantly infected mice led to short subclinical infections. if infection occurred in early pregnancy, the young appeared normal at birth but their tissues were highly infective. for two to three months their bites and urine were also highly infective. a viraemia did not persist long enough for successive vertical transmissions of the infection to be likely. however, the viruria persisted in most prenatally infect ... | 1976 | 1063218 |
| lymphocytic choriomeningitis virus. ii. characterization of extractable complement-fixing activity. | lymphocytic choriomeningitis (lcm) virus-specific complement-fixing (cf) antigen (ecfa) has been solubilized, concentrated, and partially purified. when inoculated together with freund's adjuvant, ecfa induced cf antibody but not neutralizing antibody or protective immunity. by itself it boosted pre-existing cf antibody but no neutralizing antibody. in double diffusion tests one line developed between ecfa and its antiserum, and a corresponding line became visible when ecfa interacted with an an ... | 1976 | 819767 |
| accelerated detection of lymphocytic choriomeningitis virus in diagnostic specimens. | lymphocytic choriomeningitis virus could be demonstrated earlier in mice inoculated with clinical specimens if the mice were sacrificed before they appeared sick for examination by the fluorescent antibody technique. in the procedure, the optimal route for inoculation was intracerebral and the best organ tested for staining was the brain. | 1976 | 788870 |
| h-2 compatibility requirement for virus-specific t cell-mediated effector functions in vivo. i. specificity of t cells conferring antiviral protection against lymphocytic choriomeningitis virus is associated with h-2k and h-2d. | adoptive immunization of recipient mice preinfected with lymphocytic choriomeningitis virus (lcmv) is mediated exclusively by virus-specific thymus-derived lymphocytes, when assayed in a short-term transfer model. protection, measured as reduction of lcmv plaque-forming units in spleens, is conferred only if donors of immune spleen cells and recipients share the k or the d region of the h-2 gene complex. i region compatibility is neither necessary nor sufficient. the f1 leads to parent combinati ... | 1976 | 794412 |
| diversity of lymphocytic choriomeningitis virus: variation due to replication of the virus in the mouse. | depending on passage history, strain we infectious lcm virus either damages l cells more or less severely or leaves them morphologically intact. correspondingly, the plaques which are formed on l cell monolayers are of different appearance, ranging from intensely turbid to clear. multiplication of lcm virus in certain mouse organs profoundly affects plaque characteristics. the brain, for instance, favours lytic variants while the spleen supports the replication of virus which forms turbid plaque ... | 1976 | 1245841 |
| early lymphoreticular viral tropism and antigen persistence. tamiami virus infection in the cotton rat. | tamiami virus was inoculated into its natural reservoir host, the cotton rat (sigmodon hispidus), and the course of infection was followed by sequential organ titrations, frozen-section immunofluorescence, and light and electron microscopy. in animals infected at 2 days of age, there was an early lymphoreticular tropism with peak concentrations of virus and viral antigen in lymph nodes, splenic white pulp, thymus, and bone marrow at 16 days postinoculation. megakaryocyte infection was early and ... | 1976 | 1249916 |
| implications, monitoring, and control of accidental transmission of lymphocytic choriomeningitis virus within hamster tumor cell lines. | 1976 | 1260753 | |
| the interplay between target organ concentrations of lymphocytic choriomeningitis virus and cell mediated immunity in baby mice. | circumstantial evidence has been presented which supports the view that the fatal lcm virus infection is due to an immunological conflict in the host animal. hitherto, this outcome of the infection has only been observed in intracerebrally infected mice. in the present study, the intraperitoneal infection in young mice was investigated and the results revealed a new example of this immunological conflict. in mice infected a few days after birth, concentrations of the virus in the brain are high, ... | 1976 | 1266623 |
| lymphocytic choriomeningitis virus infection in fetal, newborn, and young adult syrian hamsters (mesocricetus auratus). | the pathogenesis of lymphocytic choriomeningitis virus infection in fetal, newborn, and young adult hamsters was studied. infected newborn hamsters initially developed a persistent viremia and viruria with titers often in excess of 10(4.0) mean infectious doses/0.03 ml of blood or urine. after week 12 two different patterns of infection became evident. approximately one-half of the hamsters eventually cleared the infection, whereas the others developed a chronic progressive and ultimalely fatal ... | 1976 | 1270139 |
| viral antibody in the cerebrospinal fluid of patients with acute central nervous system infections. | cerebrospinal fluid (csf) and sera from 129 patients of a study population of 139 were tested for antibody to herpes simplex, measles,and mumps viruses. herpes simplex virus antibody was found in three of five patients with laboratory-confirmed herpes simplex infection and in eight patients without serological or virological evidence of current infection with this or other common neurotropic visuses. eleven of the 139 patients were studied for antibody to lymphocytic choriomeningitis (lcm) virus ... | 1976 | 177451 |
| immunologic mechanisms in the pathogenesis of virus-induced murine leukemia. iii. target cell specificity of autoreactive thymocytes. | thymocytes from preleukemic mice persistently infected with moloney murine leukemia virus (mulv-m-carriers) were vigorously autoaggressive toward normal syngeneic target cells; they exhibited a graded response to allogeneic cells, but they spared xenogeneic cells or syngeneic cells infected with mulv-m or mulv-g (gross). syngeneic target cells infected with nononcogenic lymphocytic choriomeningitis virus (lcmv), or transformed by the chemical carcinogen 3-methylcholanthrene were not similarly sp ... | 1976 | 180178 |
| [mutual stimulation of oncornavirus and herpetic infections in experiments in vivo and in vitro]. | the evidence of mutual stimulation of herpes simplex virus (hsv) and oncornaviruses (ov) reproduction was obtained. hsv titers in mice pre-infected with rauscher leukosis virus (rlv) were 2.25-3.0 lg ld50 higher than in mice of the same batch but without rlv infection. the continuous hep-2 culture chronically infected with oncornaviruses was found to be more sensitive to the cytopathic effect of hsv strains, types 1 and 2, and in the culture fluid hsv accumulated to a greater titer than in prima ... | 1976 | 193302 |
| specific immune lysis of paramyxovirus-infected cells by h-2-compatible thymus-derived lymphocytes. | mice exposed to paramyxovirus (sendai) generate specifically sensitized thymus-derived lymphocytes (t cells) which, in an in vitro 51cr release assay, interact only with virus-infected target cells sharing strong transplantation antigens. reciprocal exclusion of cytotoxic t-cell activity is found for sendai virus, lymphocytic choriomeningitis virus and ectromelia virus. immune t cells are detected as early as 3 days after intraperitoneal inoculation with a large dose of sendai virus, and cytotox ... | 1976 | 194830 |
| glomerular deposition of ss protein in murine immune complex glomerulonephritis. | 1976 | 133012 | |
| cell-mediated cytotoxicity against sendai-virus-infected cells. | after injection of sendai virus, a parainfluenza virus type i, mice generate cytotoxic lymphocytes which lyse specifically sendai-virus-infected target cells in vitro. their action is not inhibited by specific antibody in vitro. killer cell activity appears 4 days after infection, reaches a maximum on the 7th day and disappears on the 14th to 16th day. decrease of cytotoxic cell activity is correlated with an increase of haemagglutinating antibodies. the cytotoxic effector cell could be characte ... | 1976 | 60844 |
| h-2 restriction of virus-specific cytotoxicity across the h-2 barrier. separate effector t-cell specificities are associated with self-h-2 and with the tolerated allogeneic h-2 in chimeras. | during infection with lymphocytic choriomeningitis or vaccinia virus, f1 irradiation chimeras reconstituted with bone marrow cells from or both parents generate cytotoxic t cells which can lyse targets across the h-2 barrier. however, activity of chimera t cells is h-2 restricted as shown by cold target competition experiments and selective restimulation of a secondary response in vitro; t cells of h-2k specificity which lyse tolerated infected h-2d target cells do not lyse infected h-2k or unre ... | 1976 | 62016 |
| shared determinants between virus-infected and trinitrophenyl-conjugated h-2-identical target cells detected in cell-mediated lympholysis. | infection of h-2-identical mice with either lymphocytic choriomeningitis (lcm) virus, vaccinia virus, or paramyxo (sendai) virus resulted in the generation of specifically sensitized cytotoxic t lymphocytes (ctl). ctl generated in vitro against 2,4,6-trinitrophenyl (tnp)-conjugated syngeneic stimulator cells were specifically cytotoxic for tnp-conjugated h-2k (d) region identical targets. both lcm and vaccinia-induced ctl, however, were found to be strongly cytotoxic towards tnp-conjugated, h-2k ... | 1976 | 63379 |
| electron-microscopic identification of infectious particles of lymphocytic choriomeningitis. | lcm virus, strain we--grown on l cells--and labeled with 3h-uridine was centrifuged to equilibrium in a sucrose density gradient and examined in fractions for infectivity, incorporated radioactivity, and electron-microscopic features. the peak of infectivity is congruent with the one of radioactivity (density = 1.17 g/ml). lcm virus specificity of the radioactive peak was proved by precipitation of the radioactivity with anti-lcm virus antiserum. the peak fractions showed an abundance of 106 +/- ... | 1976 | 63905 |
| the cell-mediated immune response to ectromelia virus infection. secondary response in vitro: specificity, nature of effector and responder cells and requirements for induction of antigenic changes in stimulator cells. | an in vitro culture method was used to study secondary cell-mediated responses to ectromelia virus infection in mice. infected, syngeneic spleen cells or peritoneal cells were efficient "stimulator" cells when cultured with "responder" cells obtained from mice infected with ectromelia 4-6 weeks previously. the kinetics of generation of cytotoxic cells in cultures were determined; a peak occurred on days 4-5. a separation procedure performed on the cytotoxic cells showed that activity was associa ... | 1976 | 65167 |
| potential pathogenic mechanisms of injury in amyotrophic lateral sclerosis. | 1976 | 66788 | |
| major transplantation antigens, viruses, and specificity of surveillance t cells. | 1977 | 69515 | |
| comparison of t cell-mediated immune responsiveness of nzb, (nzb x &nzw)f1 hybrid and other murine strains. | the age-dependent capacity of nzb and (nzb x nzw)f1 hybrid, balb/c, dba/2, c57bl/6 and c3h mice to generate t cell-mediated immune responses was assessed qualitatively and quantitatively by measuring the following effector functions: (a) the time course of alloreactive cytotoxic t-cell activity triggered in vitro was comparable for nz and other mouse strains; cell reactivity generated in vivo against el4 tumour cells was low in young (nzb x nzw)f1 mice and in dba/2 mice but was comparable for ol ... | 1977 | 142594 |