Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| treatment of respiratory viral infection in an immunodeficient infant with ribavirin aerosol. | an infant with severe combined immunodeficiency syndrome (scids) secondary to adenosine deaminase deficiency had pneumonitis and combined infection with respiratory syncytial virus (rsv) and parainfluenza virus type 3 (piv3). four separate courses of ribavirin were delivered by small-particle aerosol. the piv3 disappeared during the first course, and rsv disappeared after the fourth course on the 58th hospital day. neither virus returned during profound immunosuppression for bone marrow transpla ... | 1984 | 6322573 |
| a simple procedure for the analysis of the structural proteins of influenza and parainfluenza viruses involving adsorption to erythrocytes. | a simple procedure for the analysis of the structural proteins of influenza and parainfluenza viruses utilizing adsorption to erythrocytes is described. the method involves virus growth in the presence of [35s]methionine, adsorption of clarified culture medium with a 0.5% suspension of either guinea-pig or chicken erythrocytes and analysis of the virus-erythrocyte aggregates by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page). all of the structural proteins can be detected u ... | 1984 | 6323512 |
| association of parainfluenza-3 virus with bovine macrophages and blood cells: an in vitro study. | bovine blood cells and peritoneal and lung macrophages were exposed in vitro to parainfluenza-3 (pi-3) virus. residual nonadsorbed pi-3 virus (expressed in percentage of input virus) in the supernate of the various cell fractions 1 hour after incubation at 37 c was as follows: lung macrophages, 11%; peritoneal macrophages, 59%; monocytes, 26%; rbc, 14%; lymphocytes, 28%; and polymorphonuclear cells (pmn), 63%. lung macrophages, monocytes, lymphocytes, and pmn were monitored over a 72-hour period ... | 1984 | 6324623 |
| a simultaneous outbreak of respiratory syncytial virus and parainfluenza virus type 3 in a newborn nursery. | over a 3-week period, 20 of 34 (59%) infants in a newborn nursery developed nosocomial viral respiratory tract disease. either respiratory syncytial virus (seven infants) or parainfluenza virus type 3 (five) or both (two) were demonstrated in respiratory secretions from 14 of the 20 symptomatic patients. symptoms in the 20 infants included rhinitis (15 infants), cough (14), apnea (eight), pulmonary infiltrates (seven), and fever (six). there were no differences in symptoms between children infec ... | 1984 | 6325655 |
| brs virus, pi3 virus and bhv1 infections of young stock on self-contained dairy farms: epidemiological and clinical findings. | the role of bovine respiratory syncytial virus, parainfluenza type 3 virus and bovine herpesvirus 1 as disease agents in 28 groups of young cattle on 19 dairy farms which raised their own replacements was investigated. bovine respiratory syncytial virus infections occurred in 27, parainfluenza type 3 virus infections in all and bovine herpesvirus 1 infections in three of the 28 groups. some infections were accompanied by clinical signs while others were entirely subclinical. clinical respiratory ... | 1984 | 6326374 |
| production of cattle immunotolerant to bovine viral diarrhea virus. | inoculation of bovine virus diarrhea virus into 58 to 125 day old fetuses of bovine virus diarrhea virus seropositive pregnant cows, or inoculation of bovine virus diarrhea virus into seronegative cows 42 to 114 days pregnant, may produce clinically normal calves which are persistently infected with the specific isolate of bovine virus diarrhea virus yet seronegative to the homologous and heterologous isolates. reinoculation of these persistently infected cattle with their homologous isolate pro ... | 1984 | 6326980 |
| evaluation of cold-adapted and temperature-sensitive mutants of parainfluenza virus type 3 in weanling hamsters. | cold-adapted (ca) and temperature-sensitive (ts) mutants of parainfluenza virus 3 were produced by serially passaging wild type (wt) parainfluenza virus 3 at 20 degrees c 45 times. previously, plaque-purified viruses (clones) were selected from the wt parent and from cold passage levels 7, 12, 18, and 45 and characterized in vitro. in the present study, we evaluated at least one mutant from each cold passage level for attenuation in hamsters. four of the five mutants tested were ts and ca (one e ... | 1984 | 6327899 |
| cell mediated immunity of calves to bovine parainfluenza-3 virus; leukocyte migration inhibition and leukocyte aggregation response. | 1984 | 6328797 | |
| prevalence of bovine herpesvirus-1, bovine viral diarrhea, parainfluenza-3, goat respiratory syncytial, bovine leukemia, and bluetongue viral antibodies in sheep. | sera from healthy sheep were collected in january and march 1982 from flocks of sheep located in southwestern and southeastern louisiana. these sera were tested for bovine herpesvirus-1 (bhv-1), bovine viral diarrhea virus (bvdv), parainfluenza-3 (pi-3) virus, and goat respiratory syncytial virus (grsv) antibodies by microtitration virus-neutralization test. the sera were tested also for bovine leukemia virus (blv) and bluetongue virus (btv) antibodies by immunodiffusion tests. the number of flo ... | 1984 | 6329044 |
| antibody and complement-mediated cytotoxicity for bovine parainfluenza-3 virus-infected cells. | an antibody-complement-mediated cytotoxicity assay was employed to determine whether susceptible host cells infected with bovine parainfluenza-3 virus could be destroyed by the humoral immune mechanism. the cytotoxicity assay yielded 21.5% to 76.9% specific release of 51cr with sera collected from calves 28 days after inoculation with the virus. a multiplicity of infection of 5, a postinfection period of 2 days, an antibody dilution of 1:10, and an incubation period of 12 hours with antibody and ... | 1984 | 6331235 |
| viral diagnoses using the rapid immunofluorescence technique and epidemiological implications of acute respiratory infections among children in different european countries. | from november 1978 to october 1981, a total of 7716 specimens of nasopharyngeal secretions were examined by the rapid immunofluorescence technique to determine the frequency of infections caused by the respiratory syncytial virus (rsv), influenza virus a, and parainfluenza viruses 1 and 3. the tests were carried out in six different virus laboratories located in newcastle upon tyne (england), copenhagen, oslo, stockholm, turku (finland), and vienna; laboratories in lisbon and paris participated ... | 1984 | 6375886 |
| broad-spectrum synergistic antiviral activity of selenazofurin and ribavirin. | the antiviral effects of selenazofurin (2-beta-d-ribofuranosylselenazole-4-carboxamide, selenazole), ribavirin (1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamide), and 3-deazaguanosine (6-amino-1-beta-d-ribofuranosylimidazo-[4.5-c]pyridin-4(5h)-one) were investigated separately and in various combinations in an in vitro study. the combination interactions were evaluated at seven drug concentrations, graphically (isobolograms) or by using fractional inhibitory concentration indices against mump ... | 1984 | 6517540 |
| the importance of imp dehydrogenase inhibition in the broad spectrum antiviral activity of ribavirin and selenazofurin. | the nucleosides ribavirin, selenazofurin, tiazofurin and bredinin all exhibit the lowering of guanylate pools in vitro and in vivo by the inhibition of imp dehydrogenase. however, each of these nucleosides has a separate profile of antiviral and antitumor activity. the imp dehydrogenase inhibition in the case of ribavirin and bredinin appears to be due to the nucleoside 5'-monophosphate and in the case of selenazofurin and tiazofurin to the nad analogs formed intracellularly. with regard to the ... | 1985 | 2872781 |
| seroepidemiologic survey for antibodies to selected viruses in the respiratory tract of lambs. | a serologic study was conducted to determine the prevalence of antibodies to, and infection rate of, mastadenovirus ovi 5, m ovi 6, parainfluenza-3 (pi-3) virus, bovine herpesvirus-1 (bhv-1), respiratory syncytial virus (rsv), bovine viral diarrhea (bvd) virus, and ovine progressive pneumonia (opp) virus in lambs at a ram lamb growth-rate test station. for 2 consecutive years, serum samples were prepared from blood collected from 1- to 2-month-old ram lambs as they entered the test station (1st ... | 1985 | 3002217 |
| [response of guinea pigs to vaccination with parainfluenza virus 3]. | an inactivated vaccine was prepared with parainfluenza-3 virus strain lq-514 and strains of pasteurella hemolytica and p. multocida, suspended in oil adjuvant. the virus had been isolated from 30-60 day old calves during an epidemic of pneumonia. the vaccine was tested in guinea pigs aged 1 to 2 months. the antibody response and the virus titres in organs after the challenge were the parameters studied. hemagglutination inhibition antibodies were first detected 14 days after vaccination and reac ... | 1985 | 2829271 |
| respiratory virus surveillance--united states, january 1985. | 1985 | 2981395 | |
| antiviral effect of flavonoids on human viruses. | the effect of several naturally occurring dietary flavonoids including quercetin, naringin, hesperetin, and catechin on the infectivity and replication of herpes simplex virus type 1 (hsv-1), polio-virus type 1, parainfluenza virus type 3 (pf-3), and respiratory syncytial virus (rsv) was studied in vitro in cell culture monolayers employing the technique of viral plaque reduction. quercetin caused a concentration-dependent reduction in the infectivity of each virus. in addition, it reduced intra ... | 1985 | 2981979 |
| kawasaki disease associated with klebsiella pneumoniae bacteremia and parainfluenza type 3 virus infection. | 1985 | 2982131 | |
| experimental parainfluenza virus infection in mice: growth and spread of a highly pathogenic variant of parainfluenza 3 virus in the mouse brain. | we had previously showed that following intracerebral inoculation of newborn mice, the 910 n and m strains of bovine parainfluenza 3 virus induce a non-lethal hydrocephalus and a lethal disease with marked thymic and splenic atrophy, respectively. moreover, only the m virus was lethal for 2-week-old mice. in the present study, we demonstrate that the m virus multiplies and spreads in the mouse brain invading the thalamus, hypothalamus and brain stem beyond the ependyma whereas the 910 n virus ca ... | 1985 | 2982350 |
| synthesis and antitumor and antiviral activities of a series of 1-beta-d-ribofuranosyl-5-halocytosine (5-halocytidine) cyclic 3',5'-monophosphates. | a series of 1-beta-ribofuranosyl-5-halocytosine cyclic 3',5'-monophosphates (1-4) has been prepared. direct halogenation of cytidine 3',5'-monophosphate (ccmp) yielded the cl, br, and i compounds while 5-f-ccmp (1) was obtained on cyclization of the 5'-monophosphate. on in vitro testing of 1-4 against l1210 and p388 leukemias, only 1 showed significant low-level activity (id50 = 3.1 x 10(-4) mmol/l). derivatives 2-4 were inactive at 10(-1) mmol/l and also proved to have low viral ratings against ... | 1985 | 2984417 |
| protection from parainfluenza-3 virus and persistence of infectious bovine rhinotracheitis virus in sheep vaccinated with a modified live ibr-pi-3 vaccine. | ewes (n = 7) and their lambs (n = 12) were vaccinated with a commercial modified live infectious bovine rhinotracheitis-parainfluenza type 3 virus vaccine. both the vaccinated ewes and lambs and a group of unvaccinated ewes (n = 8) and their lambs (n = 13) were subsequently challenged with virulent parainfluenza type 3 virus. although absolute immunity to infection and clinical response was not conferred, the clinical response was less severe in vaccinated lambs. vaccinated animals also shed par ... | 1985 | 2985216 |
| a serologic epidemiological study of parainfluenza 1, 2, and 3 viruses in beef cattle and calves in illinois. | beef cattle and calves in four illinois populations were studied. serum was tested by the hemagglutination-inhibition (hi) test for antibodies to parainfluenza viruses (piv) 1, 2, and 3. in all but group 3 a low level of antibody to piv-1 and 2 was demonstrated. the highest prevalence and incidence of hi-antibodies was found for piv-3, followed by piv-1. there may have been a cross-reaction between piv-1 and -3 in group 1 calves. the low level of hi antibodies to piv-1 and 2 may have resulted fr ... | 1985 | 2986256 |
| properties of temperature-sensitive mutants of parainfluenza virus type 3 selected during the course of persistent infection. | we investigated properties of the ts mutants that were selected during the course of persistent infection of vero cells by parainfluenza virus type 3. the mutants demonstrated leakiness when infecting cells at high moi and interfered with the growth of wild type virus, apparently by inhibiting a step prior to rna synthesis. | 1985 | 2986583 |
| intracellular synthesis of human parainfluenza type 3 virus-specified polypeptides. | the intracellular synthesis of human parainfluenza type 3 virus-specified polypeptides was examined by polyacrylamide gel electrophoresis of [35s]methionine-labeled cell extracts under reducing conditions. all of the virion structural proteins were detected in cell extracts, including: l, 180,000 molecular weight (180k); p, 83k; hn, 69k; np, 66k; f0, 60k; f1, 51k; and m, 38k. p and np were phosphorylated. hn and f were glycosylated. the kinetics of intracellular viral protein synthesis did not d ... | 1985 | 2987519 |
| serum antibody to respiratory syncytial virus in goats in the uk. | 1985 | 2988182 | |
| inability to detect a k cell in bovine peripheral blood leukocytes. | antibody-dependent cellular cytotoxicity to viral-infected cells, chicken red blood cells, and tumor cells was tested using different effector cell populations: polymorphonuclear cells, mononuclear cells, and mononuclear cells separated into adherent and nonadherent populations by sephadex g-10. polymorphonuclear cells were the most efficient mediators of antibody-dependent cellular cytotoxicity against most targets, although a combination of g-10 adherent and polymorphonuclear cells was more ef ... | 1985 | 2988185 |
| surveillance of respiratory viral infections by rapid immunofluorescence diagnosis, with emphasis on the epidemiological development of respiratory syncytial virus infections. | surveillance of certain respiratory viral infections by applying immunofluorescence (if) examinations to samples of nasopharyngeal secretions has been evaluated using a simplified procedure for the preparation of cell smears. samples from 711 children living in different parts of norway were examined during the winter 1982/83 and a positive diagnosis was made for 290 children (41%). temporal epidemic peaks were observed for respiratory syncytial virus (rsv), parainfluenza virus type 3 and influe ... | 1985 | 2989359 |
| in vivo and in vitro studies on the use of the guinea pig as a model for virus-provoked airway hyperreactivity. | the parainfluenza 3 (p-3)-infected guinea pig was examined as a model for virus-provoked airway hyperreactivity by measuring changes in airway overflow pressure in response to intravenously (iv) administered histamine. in vitro responses of lung parenchymal strips to several contractile substances were also measured. all studies were conducted 4 days after nasal insufflation with p-3 or p-3 growth medium (control). increases in airway overflow pressure caused by histamine were enhanced by p-3 in ... | 1985 | 2992331 |
| synthesis and biological activity of 5-thiobredinin and certain related 5-substituted imidazole-4-carboxamide ribonucleosides. | a number of 5-substituted imidazole-4-carboxamide ribonucleosides were prepared and tested for their biological activity. treatment of 5-chloro-1-beta-d-ribofuranosylimidazole-4-carboxamide (2) with methanethiol provided 5-(methylthio)-1-beta-d-ribofuranosylimidazole-4-carboxamide (3a). similar treatment of 2 with ethanethiol or benzenemethanethiol gave the corresponding 5-ethylthio and 5-benzylthio derivatives 3b and 3c. oxidation of 3a and 3b with m-chloroperoxybenzoic acid furnished the corre ... | 1985 | 2993613 |
| proteins associated with human parainfluenza virus type 3. | the polypeptides associated with human parainfluenza virus type 3 were identified. five proteins were present in detergent- and salt-resistant viral cores. of these, three proteins designated np0, np1, and np2 of 68,000, 58,000, and 52,000 daltons, respectively, were stably associated with 50s rna in cscl gradient-purified nucleocapsids. the amounts of np1 and np2 were variable, and these proteins were shown to be structurally related to the major nucleocapsid protein (np0) by partial staphyloco ... | 1985 | 2993658 |
| impaired function of bovine alveolar macrophages infected with parainfluenza-3 virus. | bovine alveolar macrophages (bam) were harvested from nonsedated cattle, adhered to glass or plastic surfaces, and infected with parainfluenza-3 (pi-3) virus at a multiplicity of infection of 10. control and pi-3 virus-infected bam were compared at 24-hour intervals up to 168 hours for their ability to phagocytize antibody-coated sheep erythrocytes (eac) and latex particles, to kill staphylococcus epidermidis, and to alter intracellular acid phosphatase concentrations. the effect of antiviral se ... | 1985 | 2994528 |
| paramyxovirus antigens in osteoclasts from paget's bone tissue detected by monoclonal antibodies. | the fluorescent antibody technique using both monoclonal and specific polyclonal virus antibodies was applied to investigate the nature of the inclusions seen in the abnormal osteoclasts associated with paget's bone disease. the results show that antigens of measles virus, simian virus 5 (sv5) and human parainfluenza virus type 3 (pf3) could be detected in the osteoclasts but not in control bone cells. measles and sv5 nucleoprotein (np) and haemagglutinin-neuraminidase (hn) antigens were apparen ... | 1985 | 2995550 |
| synthesis and biological activity of certain 6-substituted and 2,6-disubstituted 2'-deoxytubercidins prepared via the stereospecific sodium salt glycosylation procedure. | a number of 6-substituted and 2,6-disubstituted pyrrolo[2,3-d]pyrimidine 2'-deoxyribonucleosides were prepared by the direct stereospecific sodium salt glycosylation procedure. reaction of the sodium salt of 4-chloro-6-methyl-2-(methylthio)pyrrolo[2,3-d]pyrimidine (6a) or 4,6-dichloro-2-(methylthio)pyrrolo[2,3-d]pyrimidine (6b) with 1-chloro-2-deoxy-3,5-di-o-p-toluoyl-alpha-d-erythro-pentofuranose (9) provided the corresponding n7 2'-deoxy-beta-d-ribofuranosyl blocked derivatives (8a and 8c) whi ... | 1985 | 2995666 |
| comparison of interferon production in cattle after intranasal infection with parainfluenza-3 live vaccine and avirulent ibr/ipv-herpesvirus. | 1985 | 2996264 | |
| rapid viral diagnosis of acute respiratory infections: comparison of enzyme-linked immunosorbent assay and the immunofluorescence technique for detection of viral antigens in nasopharyngeal secretions. | nasopharyngeal secretions from adults and children were obtained in stockholm, sweden, for routine diagnosis of influenza a virus, influenza b virus, respiratory syncytial (rs) virus, parainfluenza type 3 virus, and adenovirus infections by demonstration of viral antigens directly in the specimens. the cells in nasopharyngeal secretions were pelleted by centrifugation for preparation of cell deposits for diagnosis by the immunofluorescence technique (if) in london, england, and in stockholm, whe ... | 1985 | 2997270 |
| studies on human parainfluenza virus 3: characterization of the structural proteins and in vitro synthesized proteins coded by mrnas isolated from infected cells. | the structural proteins of human parainfluenza virus 3, a member of the paramyxovirus family, were characterized by sds-polyacrylamide gel electrophoresis of radiolabeled virus. the purified virion contains at least eight structural proteins, with estimated molecular weights of 251k, 90k, 71k, 68k, 65k, 51k, 35k, and 21k, respectively. three of the polypeptides (71k, 65k, and 51k) were identified as glycoproteins based on their incorporation of [3h]glucosamine. disruption of the virus by triton ... | 1985 | 2998013 |
| cloning and gene assignment of mrnas of human parainfluenza virus 3. | cytoplasmic poly(a)-containing rna from parainfluenza virus 3-infected cells was used as template to construct a cdna library that was cloned into the ecorv site of the plasmid pmg5. the resulting clones were screened with [32p]-labeled cdna probes made from infected and mock-infected cell mrnas. the virus specificity of the clones was confirmed by northern blot hybridization. the viral clones were grouped into five different families by hybridization with individual size-selected reverse transc ... | 1985 | 2998060 |
| prevalence of antibodies to bovid herpesvirus 1 (ibr-ipv), bovine virus diarrhoea, bovine respiratory syncytial parainfluenza 3, adeno a and adeno b viruses in indigenous and imported moroccan cattle. | a serological survey on prevalence of antibodies to bovid herpesvirus 1 (ibr-ipv) bovine virus diarrhoea, bovine respiratory syncytial, parainfluenza 3, adeno a and b viruses was performed in 524 cattle from different areas and management conditions in morocco. general antibody prevalence was 62.8, 48.5, 70.4, 68.1, 9.0 and 12.4, for the six viruses, respectively. higher prevalence and high antibody titers to most of the investigated viruses were found among cattle from extensive management syst ... | 1985 | 2998260 |
| [studies on the pathogenesis of subacute thyroiditis]. | despite the fact that subacute thyroiditis (sat) is a common disease, its pathogenesis is still uncertain. at present, viral infection is simply thought to be a possible pathogenic factor. in caucasians and chinese, however, a strong association has recently been found between hla-bw35 and sat. therefore, genetic factors are also thought to participate in the onset of sat. in this study we have further attempted to clarify the pathogenesis of this disease in this regard. protocol: hla typing for ... | 1985 | 2998895 |
| in vitro protective effect of bacteria-derived bovine alpha interferon i1 against selected bovine viruses. | we used bacteria-derived bovine alpha-interferon i1 (bo ifn-alpha i1) to study its antiviral effect in a bovine turbinate cell line on bovine diarrhea virus, infectious bovine rhinotracheitis virus, parainfluenza 3 virus, and pseudorabies virus. we based our study upon replicate tests for each strain by using a block titration system with various concentrations of bo ifn-alpha i1 against various concentrations of virus. the data were compiled in two-axis tables (replicate x concentration) and we ... | 1985 | 2999188 |
| glycoproteins of human parainfluenza virus type 3: characterization and evaluation as a subunit vaccine. | the envelope glycoproteins of human parainfluenza type 3 virus were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and reactivity with specific monoclonal antibodies. the molecular weight of the hemagglutinin-neuraminidase (hn) glycoprotein was found to be 72,000, and the fusion (f) glycoprotein appeared to consist of 74,000 (f0) or 56,000 (f1) species. envelope glycoproteins were solubilized with octyl-glucoside and, after removal of the detergent by dialysis, were u ... | 1985 | 2999259 |
| microwave sterilization of nitrous oxide nasal hoods contaminated with virus. | although there exists a desire to eliminate the possibility of cross-infection from microbial contaminated nitrous oxide nasal hoods, effective and practical methods of sterilization in a dental office are unsatisfactory. microwaves have been used to sterilize certain contaminated dental instruments without damage. in this study nasal hoods contaminated with rhinovirus, parainfluenza virus, adenovirus, and herpes simplex virus were sterilized in a modified microwave oven. ninety-five percent of ... | 1985 | 2999676 |
| persistence of viruses in the nasopharynx of apparently healthy children aged 0-5 years. results of investigations performed in 1982-83. | virus isolation attempts were made with nasopharyngeal secretions from 400 apparently healthy 0-5-year-old children of a semi-closed community. repeated virus isolations were achieved in 25 cases subjected to periodic investigations in 1982-83. one-two reisolation(s), 1-5 months apart, of the initially detected viral agents could be obtained in 15 out of the 25 children. the following viruses were reisolated (in decreasing order of frequency): adenoviruses, respiratory syncytial virus, coxsackie ... | 1985 | 3000066 |
| human parainfluenza virus 3: purification and characterization of subviral components, viral proteins and viral rna. | a simple method was established that allowed large quantities of human parainfluenza 3 (pf3) virions to be isolated from tissue culture cells. the purity of the virus was sufficient for biochemical analysis of virion proteins. the density of pf3 virions was 1.18-1.20. purified virions contained seven viral proteins with estimated molecular weights of: l, 180 000; p, 83 000; hn, 69 000; np, 66 000; f0, 60 000; f1, 51 000; and m, 38 000, as determined by sodium dodecyl sulfate-polyacrylamide gel e ... | 1985 | 3000105 |
| epizootiological survey of parainfluenza-3, reovirus-3, respiratory syncytial and infectious bovine rhinotracheitis viral antibodies in sheep and goat flocks in quebec. | a serological survey was conducted in an attempt to detect antibodies against bovine respiratory viruses in sheep and goats from seven geographical areas of quebec. sera from 10% of the animals in 182 sheep flocks and 40 goat flocks were collected and specific antibodies against parainfluenza-3, reovirus type 3, respiratory syncytial and infectious bovine rhinotracheitis viruses were detected by hemagglutination-inhibition tests for the former viruses and complement fixation and seroneutralizati ... | 1985 | 3000551 |
| a simple method for increased recovery of purified paramyxovirus virions. | a simple method involving vigorous agitation of infected cell monolayers prior to collection of culture medium is described to greatly increase the recovery of purified virions of measles virus, respiratory syncytial virus and human parainfluenza virus. vigorous agitation of the flasks containing monolayers of infected cells increased the recovery of purified virions by at least 3- to 10-fold as judged by the intensity of [35s]methionine labeled viral proteins on sodium dodecyl sulphate-polyacry ... | 1985 | 3001119 |
| production of monoclonal antibodies against parainfluenza 3 virus and their use in diagnosis by immunofluorescence. | monoclonal antibodies were produced against parainfluenza virus type 3 (pi-3) and used to identify pi-3 clinical isolates in cell culture and pi-3 antigen in cells obtained from nasopharyngeal (np) washes of patients. two (2e9 and 4g5) of the three monoclonal antibodies characterized reacted by immunoblotting with a 67,000-dalton pi-3 protein, and one antibody (4e5) reacted with two viral proteins in the range of 29,000 to 31,000 daltons. the three monoclonal antibodies did not cross-react by in ... | 1985 | 3001131 |
| a survey of respiratory syncytial virus and parainfluenza virus type 3 neutralising and immunoprecipitating antibodies in relation to paget disease. | the aetiology of paget disease of bone has not been established but certain features have suggested involvement of a parainfluenzalike virus. to seek further evidence of the possible role of paramyxoviruses in paget disease we have surveyed the presence of neutralising and immunoprecipitating antibodies to both respiratory syncytial virus and parainfluenza virus type 3 in the sera of patients attending a bone disease clinic. these two viruses were implicated by the sporadic observation of viral ... | 1985 | 3001223 |
| application of computer-assisted image analysis for studying viral structures. | the application of caie has been shown to be useful for analyzing the structures of rna viruses. critical assessment of this method is essential for the selection of the micrographs of viruses. in our experience this procedure was helpful for resolving some questions concerning virus morphology. | 1985 | 3006847 |
| antigenic variation in the hemagglutinin-neuraminidase protein of human parainfluenza type 3 virus. | sixteen monoclonal antibodies directed to the hemagglutinin-neuraminidase (hn) protein of a 1957 isolate of parainfluenza type 3 virus (piv3) were produced and used to examine antigenic variation in clinical strains. analysis of hemagglutination-inhibition reactivity patterns of antigenic variants selected in vitro in the presence of monoclonal antibodies indicated that there were a minimum of six distinct epitopes detectable on the hn molecule. competitive-binding assays indicated that these ep ... | 1985 | 2414910 |
| monoclonal antibodies reveal extensive antigenic differences between the hemagglutinin-neuraminidase glycoproteins of human and bovine parainfluenza 3 viruses. | a panel of twenty monoclonal antibodies to the hemagglutinin-neuraminidase (hn) glycoprotein of human parainfluenza 3 (pi3) virus has been obtained and tentatively classified into four different groups based on reactivity in hemagglutination inhibition (hi), neuraminidase inhibition (ni), and plaque neutralization (nt) tests. the antibodies were tested for cross-reactivity with bovine pi3 virus, sendai virus, and simian virus 5 (sv5). only two of these antibodies showed similar reactivities with ... | 1986 | 2417412 |
| molecular cloning and sequence analysis of the human parainfluenza 3 virus rna encoding the nucleocapsid protein. | the sequence of 1690 nucleotides from the 5' end of the viral complementary rna for the human parainfluenza 3 virus was determined by molecular cloning. one large open reading frame consisting of 1548 nucleotides was demonstrated. the encoded protein, the nucleocapsid protein (np), consists of 515 amino acids, and has a predicted molecular weight of 57,819. a noncoding 5' sequence of 51 nucleotides is present at the end of the np-mrna. two consensus sequences were identified which are homologous ... | 1986 | 2418584 |
| characterization of four parainfluenza virus type 3 proteins by use of monoclonal antibodies. | monoclonal antibodies directed against four structural components of the atcc strain c243 of parainfluenza virus type 3 were produced. the specific reaction of the antibodies with individual structural components was determined by radioimmune precipitation assay. in the collection of monoclonal antibodies, 21 reacted with the haemagglutinin-neuraminidase (hn) glycoprotein (mol. wt. 72,000), eight with the fusion (f) glycoprotein (mol. wt. 64,000), 27 with the nucleocapsid (np) protein (mol. wt. ... | 1986 | 2426392 |
| antibodies to bovine bacterial and viral pathogens in pronghorns in alberta, 1983. | sera from 210 pronghorns (antilocapra americana) ranging in southeastern alberta were tested for antibodies to disease agents present in indigenous cattle. no antibodies to brucella abortus, leptospira interrogans serovars pomona, hardjo, or grippotyphosa, or infectious bovine rhinotracheitis virus were found. antibodies at prevalences of 43.8% and 49.2% were detected to bovine virus diarrhea (bvd) and parainfluenza type 3 (pi-3) viruses, respectively. the much higher prevalence of bvd virus ant ... | 1986 | 2845155 |
| [varying nature of the cell receptors for influenza and para-influenza viruses]. | a comparative study of receptors for influenza virus, fowl plague virus, and human parainfluenza type 3 virus was carried out. natural receptors of guinea pig erythrocytes were destroyed with neuraminidase, and individual gangliosides gm1, gd1a, and gt1b were inserted into their membranes. the labeled virus was adsorbed on the erythrocytes modified in this manner, and the degree of restoration of the receptor activity of erythrocytes lost after neuraminidase treatment was determined. two ganglio ... | 1986 | 3008439 |
| [structural organization and proteins of the human type-3 para-influenza virus]. | the structure of human parainfluenza type 3 virus was studied by electron microscopy and virion fractionation by treatment with a detergent and high ionic strength. the protein spectrum of the virus was studied. the presence of 6 structural proteins was revealed of which two, hn and f, are glycoproteins. intracellular cleavage of f0 protein into f1+2 proteins was demonstrated in a pulse-chase experiment. a tighter binding of hn protein than of f protein with the virus lipoprotein membrane was ob ... | 1986 | 3008440 |
| effect of recombinant dna-derived bovine and human interferons on replication of bovine herpesvirus-1, parainfluenza-3, and respiratory syncytial viruses. | antiviral effects of recombinant dna-derived bovine (bo) and human (hu) interferons (ifn) on the replication of bovine herpesvirus-1, parainfluenza-3, and respiratory syncytial viruses were studied. bovine monolayer cultures were treated with recombinant dna-produced bo ifn-alpha 1, bo ifn-beta 2, hu ifn-alpha a, or hu ifn-alpha a/d and then challenge exposed with bovine herpesvirus-1, bovine parainfluenza-3 virus, bovine respiratory syncytial virus, or vesicular stomatitis virus. treatment with ... | 1986 | 3008606 |
| rapid diagnosis of respiratory virus infections in patients with acute respiratory disease. | viral respiratory infections represent a significant segment of the total respiratory disease spectrum; however, until recently the laboratory diagnosis of viral respiratory infections was relatively inefficient. development of new and improved immunologic assay systems has paved the way for accurate and reliable rapid diagnostic tests that detect viral antigens in clinical specimens. we conducted a careful and elaborate study in which radioimmunoassay for antigen detection was compared with a b ... | 1986 | 3009083 |
| the effect of parainfluenza virus type 3 on the phagocytic cell response of the ovine lung to pasteurella haemolytica. | groups of caesarean-derived, colostrum-deprived lambs were inoculated by the intratracheal route with pasteurella haemolytica 4 to 6 days after the inoculation of parainfluenza virus type 3 (pi3). some were killed immediately (0 h) and others 24 h later. control groups were inoculated with pi3 alone, p. haemolytica alone or media alone. pulmonary phagocytic cells, p. haemolytica and pi3 were recovered by pulmonary lavage. the phagocytes were separated into alveolar macrophage (am) and neutrophil ... | 1986 | 3010545 |
| 3'-terminal sequence of human parainfluenza virus 3 genomic rna. | 1986 | 3012476 | |
| conserved structures among the nucleocapsid proteins of the paramyxoviridae: complete nucleotide sequence of human parainfluenza virus type 3 np mrna. | the nucleotide sequence of the mrna coding for the nucleocapsid protein (np) of the paramyxovirus, human parainfluenza virus type 3 (piv-3), has been determined. the np mrna was found to contain 1642 bases, excluding poly(a), and encode a protein of 515 amino acids, with a molecular weight of 57,823. amino acid residues 1 through 420 of piv-3 np protein showed extensive sequence homology with the corresponding amino acids of sendai virus nucleocapsid protein. there was virtually no homology betw ... | 1986 | 3012866 |
| fusion glycoprotein of human parainfluenza virus type 3: nucleotide sequence of the gene, direct identification of the cleavage-activation site, and comparison with other paramyxoviruses. | the complete sequences of the fusion (f) mrna and protein of human parainfluenza virus type 3 (pf3) were determined from overlapping cdna clones. to confirm the cdna sequence, the complete sequence of the f gene was determined independently by dideoxynucleotide sequencing of genomic rna using synthetic oligonucleotide primers. the mrna contains 1845 nucleotides, exclusive of poly (a), has an unusually long (193-nucleotide) 5' nontranslated region, and encodes an f0 protein of 539 amino acids. th ... | 1986 | 3012869 |
| enzyme linked immunosorbent assay used to monitor serum antibodies to bovine respiratory disease viruses. | an enzyme linked immunosorbent assay (elisa) was applied to the detection of serum antibodies against infectious bovine rhinotracheitis (ibr), parainfluenza-3 (pi3), adenovirus type 3 (adeno 3) and bovine respiratory syncytial (brs) viruses. paired serum samples from calves vaccinated with live attenuated virus vaccines were tested. the elisa compared favorably with the virus neutralization test for detecting serologic responses to ibr, brs, and adeno 3 viruses or with the hemagglutination inhib ... | 1986 | 3014711 |
| human parainfluenza virus type 3: messenger rnas, polypeptide coding assignments, intergenic sequences, and genetic map. | cdna clones of mrnas for the major nucleocapsid protein (np), the nucleocapsid p protein plus the nonstructural c protein (p+c), and the matrix protein (m) of human parainfluenza virus type 3 (pf3) were identified by hybrid arrest and hybrid selection of in vitro translation. previously, cdna clones were identified and sequenced for the hemagglutinin-neuraminidase glycoprotein (hn) and the fusion glycoprotein (f) mrnas (n. elango, j. e. coligan, r. c. jambou, and s. venkatesan, j. virol. 57:481- ... | 1986 | 3016327 |
| messenger rna encoding the phosphoprotein (p) gene of human parainfluenza virus 3 is bicistronic. | the complete nucleotide sequence of the phosphoprotein (p) mrna of human parainfluenza virus 3 (piv-3) was derived from two cdna clones spanning almost the entire p gene. the mrna, excluding the poly(a) tail, is 2014 nucleotides long and is bicistronic. the first open reading frame (orf) codes for the phosphoprotein (p) of mol wt 68,860. seven nucleotides downstream from the first aug codon, in a +1 reading frame, there is an additional orf which can code for a polypeptide of mol wt 23,266. the ... | 1986 | 3016995 |
| induction of immunity against pneumonic pasteurellosis following experimental infection in calves. | immunity against pneumonic pasteurellosis was studied in calves after recovery from experimental respiratory disease with pasteurella haemolytica. nine calves were exposed to aerosols of parainfluenza-3 virus and pasteurella haemolytica a1 six days apart to produce respiratory disease. after recovery from the disease, these nine principal and four control calves were challenged with aerosols of bovine herpesvirus 1 and p. haemolytica a1 four days apart. with this viral-bacterial challenge, the n ... | 1986 | 3017526 |
| the association between serological titers in infectious bovine rhinotracheitis virus, bovine virus diarrhea virus, parainfluenza-3 virus, respiratory syncytial virus and treatment for respiratory disease in ontario feedlot calves. | a seroepidemiological study of the association between antibody titers to infectious bovine rhinotracheitis, parainfluenza-3, bovine virus diarrhea and bovine respiratory syncytial viruses, and treatment for bovine respiratory disease was conducted. a total of 322 calves from five different groups were bled on arrival, then one month later all cases (cattle treated for bovine respiratory disease) were rebled together with an equal number of controls (cattle not treated for any disease). titers t ... | 1986 | 3017529 |
| immunological relationships between mumps virus and parainfluenza viruses studied with monoclonal antibodies. | the immunological relationships between mumps virus and parainfluenza viruses were investigated with 74, 78 and 80 previously developed monoclonal antibodies directed against five major structural proteins of mumps virus, sendai virus (a murine parainfluenza type 1 virus) and parainfluenza type 3 virus. these monoclonal antibodies were reacted with the three viruses, with parainfluenza type 2 virus and with newcastle disease virus (ndv) in elisa and immunofluorescence (if) tests. in addition, im ... | 1986 | 3018131 |
| the effect of parainfluenza virus type 3 and pasteurella haemolytica on oxygen-dependent and oxygen-independent bactericidal mechanisms of ovine pulmonary phagocytic cells. | groups of caesarean-derived, colostrum-deprived lambs were inoculated by the intratracheal route with pasteurella haemolytica, either alone or 4 or 6 days after the inoculation of parainfluenza virus type 3 (pi3). other groups were inoculated with pi3 followed by veal infusion broth, or with uninfected cell culture fluid followed by veal infusion broth (controls). all lambs were killed 24 h after the second inoculation. pulmonary phagocytic cells were recovered by lavage and separated into alveo ... | 1986 | 3018994 |
| [use of proteolysis inhibitors for treating parainfluenza infections in an experiment and in the clinic]. | 1986 | 3020805 | |
| seroepidemiological study of para-influenza viruses in preschool children in ghana. | 1986 | 3021968 | |
| molecular cloning and sequence analysis of the human parainfluenza 3 virus mrna encoding the p and c proteins. | the sequence of the mrna encoding the phosphoprotein (p protein) of the human parainfluenza virus 3 (pf3) was determined by molecular cloning. in other parmyxoviridae the p protein mrna is functionally bicistronic and encodes an additional smaller nonstructural protein termed c. in this report three open reading frames (orf) are described. these consist of a single long orf encoding the p protein, and two shorter orfs encoding the structural vp18 protein (analogous to the sendai c protein) and a ... | 1986 | 3022477 |
| synthesis and antiviral evaluation of 1,4-dioxane nucleoside analogues related to nucleoside dialdehydes. | since biologically active nucleoside 2',3'-dialdehydes exist as six-membered cyclic acetals (1) in solution, we have investigated the antiviral activity of some structurally similar 1,4-dioxane nucleoside analogues. by reacting 2',3'-seconucleoside tosylates with base, the guanine (10) and adenine (18) substituted (hydroxymethyl)dioxanes have been constructed. in addition, an unusual adenine-substituted divinyl ether (22) was synthesized via a base-catalyzed, double elimination of a 2',3'-di-o-t ... | 1986 | 3023613 |
| parainfluenza virus type 3 in pigs. | 1986 | 3024379 | |
| differences in bovine parainfluenza 3 virus variants studied by monoclonal antibodies against viral glycoproteins. | we previously showed that of three bovine parainfluenza 3 virus strains the m strain, which is neurovirulent for young mice, has an extensive syncytium-inducing activity, whereas avirulent sc and 910n strains are weak in this activity. it was also demonstrated that both m and sc strains have very low hemagglutination and neuraminidase activities, while the 910n strain shows these activities to high levels. in the present study, monoclonal antibodies (mabs) were raised against the glycoproteins o ... | 1986 | 3024407 |
| reduction in in vivo testing at the national veterinary services laboratories of the united states department of agriculture. | efforts are being made at the national veterinary services laboratories to reduce in vivo testing of usda licensed veterinary vaccines. a hemagglutination test for determining potency of killed parvovirus vaccine is currently being used for canine and swine adjuvanted and nonadjuvanted products; a serum neutralization inhibition test (snit) is being developed for potency testing of killed adjuvanted infectious bovine rhinotracheitis (ibr), bovine virus diarrhea (bvd) and parainfluenza (pi3) vacc ... | 1986 | 3025035 |
| sequence analysis of the p and c protein genes of human parainfluenza virus type 3: patterns of amino acid sequence homology among paramyxovirus proteins. | the complete nucleotide sequence of the p + c mrna of human parainfluenza virus type 3 (pf3) was determined by sequencing cdna, viral genomic rna and mrna. the p + c mrna is 2009 nucleotides in length, exclusive of poly(a), and contains two overlapping open reading frames (orfs). the p + c mrna encodes two proteins, the 602 amino acid nucleocapsid phosphoprotein p and the 199 amino acid non-structural protein c. peptide mapping confirmed that the two proteins are unrelated. hybrid-arrest transla ... | 1986 | 3025346 |
| a bovine respiratory virus vaccination trial. | a respiratory virus vaccination trial was carried out in a commercial calf-rearing unit with a history of virus pneumonia. the effects of vaccination on the incidence of virus respiratory disease and growth rate were assessed. forty-four bought-in calves were allocated to groups and treated as follows: a, unvaccinated controls; b, intranasal temperature-sensitive infectious bovine rhinotracheitis (ibr) vaccine at three and 10 weeks; c, intranasal temperature-sensitive combined ibr and parainflue ... | 1986 | 3026078 |
| [in vitro transcription of human influenza and parainfluenza viruses and its regulation]. | the effect of some factors on in vitro transcription of human influenza and parainfluenza viruses was studied. dinucleotide afg was shown to stimulate transcription of rnp of human parainfluenza type 3 virus and sendai virus in the presence of magnesium but not manganese ions same as in the case of influenza viruses transcription. among two monoclonal antibodies to np protein of influenza virus, clone f 81 inhibited transcription of rnp of human influenza a viruses, and its influence on transcri ... | 1986 | 3026092 |
| [viral etiology of acute respiratory infections in madagascan children]. | a total of 80 nasopharyngeal secretions collected from malagasy children (53 boys and 27 girls) with viral acute respiratory infection, aged from 6 days to 10 year old admitted to the pediatric department of antananarivo general hospital from may to july 1983, were investigated by indirect immunofluorescence method. 54--samples were found positive for respiratory viruses. distribution according age groups and sex has been studied: children belonging to 25-36 month age group and male sex were mor ... | 1986 | 3026268 |
| immunofluorescence in the serological diagnosis of parainfluenza type 3 and respiratory syncytial virus infection in calves. | the fluorescent antibody (fa) test is compared with the haemagglutination inhibition (hi) test for parainfluenza virus type 3 (pi-3) and virus neutralisation (vn) test for respiratory syncytial (rs) virus for detection and titration of virus-specific antibodies. in experimentally inoculated calves pi-3 and rs virus fa tests detected seroconversion at the same time as hi and vn tests, however, in serially diluted sera, the fa test was positive to higher dilution. in studies with paired samples fr ... | 1986 | 3027802 |
| isolation of parainfluenza-3 virus from bull's semen. | 1986 | 3027942 | |
| parainfluenza virus bronchiolitis. epidemiology and pathogenesis. | an investigation of the epidemiology and pathogenesis of bronchiolitis due to parainfluenza virus (pv) was carried out. bronchiolitis due to pv occurred most commonly in non-caucasian males. breast-fed infants exhibited a reduced risk of developing bronchiolitis. once an episode of pv bronchiolitis occurred, both exposure to cigarette smoke and bottle feeding were associated with an increased frequency of recurrent wheezing, and subsequent infection with respiratory virus almost uniformly result ... | 1986 | 3002169 |
| human parainfluenza type 3 virus hemagglutinin-neuraminidase glycoprotein: nucleotide sequence of mrna and limited amino acid sequence of the purified protein. | the nucleotide sequence of mrna for the hemagglutinin-neuraminidase (hn) protein of human parainfluenza type 3 virus obtained from the corresponding cdna clone had a single long open reading frame encoding a putative protein of 64,254 daltons consisting of 572 amino acids. the deduced protein sequence was confirmed by limited n-terminal amino acid microsequencing of cnbr cleavage fragments of native hn that was purified by immunoprecipitation. the hn protein is moderately hydrophobic and has fou ... | 1986 | 3003381 |
| protection of weanling hamsters from experimental infection with wild-type parainfluenza virus type 3 (para 3) by cold-adapted mutants of para 3. | parainfluenza virus type 3 (para 3) was adapted to replicate at 20 degrees c, a nonpermissive temperature for wild-type (wt) para 3. serial passage at 20 degrees c resulted in the generation of cold-adapted (ca) and temperature-sensitive (ts) mutants. these mutant viruses have been characterized both in vitro and in vivo [belshe and hissom (1982): journal of medical virology 10:235-242; crookshanks and belshe (1984): journal of medical virology 13:243-249]. we now report the evaluation of three ... | 1986 | 3005486 |
| occurrence of antibodies to the etiologic agents of infectious bovine rhinotracheitis, parainfluenza 3, leptospirosis, and brucellosis in white-tailed deer in minnesota. | a serologic survey was conducted on 628 white-tailed deer (odocoileus virginianus) in 1976 and 1979-1980. tests for antibodies to the etiologic agents of infectious bovine rhinotrancheitis (ibr), parainfluenza 3 (pi3), leptospirosis, and brucellosis produced positive results of 15%, 20%, 3% and 0%, respectively. adult deer had significantly higher prevalence of antibodies to ibr virus and pi3 virus than fawns. these data provide a basis for monitoring these disease agents in minnesota's white-ta ... | 1986 | 3005666 |
| [a case of myocardial infarction in an 11-year-old girl with myocarditis]. | 1986 | 3035474 | |
| complete sequence of the major nucleocapsid protein gene of human parainfluenza type 3 virus: comparison with other negative strand viruses. | the sequence of the major nucleocapsid protein (np) mrna and its encoded protein were deduced by sequencing a cdna clone representing the complete mrna. the cdna sequence was confirmed by dideoxynucleotide sequencing of purified viral genomic rna by primer extension using synthetic oligonucleotides. the np mrna contains 1,641 nucleotides exclusive of poly(a) and encodes an np protein of 515 amino acids. alignment of the human parainfluenza type 3 virus (pf3) np protein sequence with that of send ... | 1986 | 2878059 |
| sequence analysis of the matrix protein gene of human parainfluenza virus type 3: extensive sequence homology among paramyxoviruses. | the sequences of the human parainfluenza virus type 3 (piv3) matrix (m) mrna [1150 nucleotides exclusive of poly(a)] and predicted m protein (353 amino acids) were determined by sequence analysis of cloned cdna and viral genomic rna. the gene-end sequence of the m gene differed from the semi-conserved gene-end sequence of the other piv3 genes by an apparent insertion of eight nucleotides. the piv3 m protein shared high sequence homology with sendai virus and moderate homology with measles virus ... | 1987 | 2883252 |
| pasteurella haemolytica serotype 1 colonization of the nasal passages of virus-infected calves. | nasal passages of calves with a virus-induced respiratory tract disease became colonized by pasteurella haemolytica serotype 1 after they were inoculated intranasally with p haemolytica. inoculation with infectious bovine rhinotracheitis virus caused a more severe clinical illness and resulted in a greater degree of colonization with p haemolytica than developed after inoculation with parainfluenza-3 virus. nasal passages of parainfluenza-3 virus-inoculated calves were colonized to a greater deg ... | 1987 | 2829666 |
| defective interfering particles of human parainfluenza virus 3. | a cyclic pattern of virus production was observed when human parainfluenza virus 3 (hpiv3) was serially passaged nine times in llc-mk2 cells. viruses produced from serial passages 8 and 9 interfered with the replication of standard hpiv3. three subgenomic rna species (di-1, di-2, and di-3) and virus genomic rna were detected in the progeny virions produced from cells mixedly infected with standard virus and virus from either serial passages 5 or 8. northern blot analysis with probes representing ... | 1987 | 3035791 |
| [proteins of the human parainfluenza virus]. | proteins of human parainfluenza type 3 virus propagated in green monkey kidney cultures are described. the presence of 4 major and 3 minor polypeptides is described along with their molecular weights. protein f1 was found as a double band, its both components having a similar intensity. only the upper band of f1 protein was found in the virus grown in vero cell culture which has a low sensitivity for this virus. fractionation of the virus and analysis of the proteins of subviral structures were ... | 1987 | 3037795 |
| antigenic analysis of human and bovine parainfluenza virus type 3 strains with monoclonal antibodies. | the antigenic characteristics of eight human strains and two bovine strains, one of which is represented by two plaque variants, of parainfluenza virus type 3 were analysed. the strains and variants were compared using 52 monoclonal antibodies against five, two, six and six epitopes of the haemagglutinin-neuraminidase (hn), fusion, nucleocapsid and matrix viral proteins respectively, employing radioimmuno-precipitation and immunofluorescence assays. the human strains, seven of which were isolate ... | 1987 | 3039045 |
| immunological interrelationships among human and non-human paramyxoviruses revealed by immunoprecipitation. | antigenic interrelationships among paramyxoviruses were examined by immunoprecipitation of isotope-labelled virus-infected cell lysates with specific antisera against virions or virus components. sendai virus, human parainfluenza virus type 1 (pa-1) and human parainfluenza virus type 3 (pa-3) belonged to one antigenic group, and human parainfluenza virus type 2 (pa-2), human parainfluenza virus type 4 (pa-4), mumps virus (muv) and simian virus 5 to a second group. furthermore, the human paramyxo ... | 1987 | 3572366 |
| complete nucleotide sequence of the matrix protein mrna and three intergenic junctions of human parainfluenza virus type 3. | the complete sequence of the gene encoding the matrix protein (m) of human parainfluenza virus type 3 (piv-3) was determined from cdna clones and from primer extension dideoxy sequencing of the viral genome. the m mrna is 1150 nucleotides in length, exclusive of polyadenylate, and codes for a protein of 353 amino acids, having a calculated molecular weight of 39,480. the m protein of piv-3 was found to have a high degree of sequence homology with that of a closely related paramyxovirus, sendai v ... | 1987 | 3027966 |
| efficiency of immunofluorescence for rapid detection of common respiratory viruses. | rapid immunofluorescence (fa) methods for the detection of common respiratory viruses were compared with culture results over a 3-year period to assess the relative efficiency of fa in a clinical laboratory setting. for respiratory syncytial virus, efficiencies were high (sensitivity, 90 to 95%; specificity, 92 to 95%). the sensitivity of fa for detection of parainfluenza virus type 1, parainfluenza virus type 3, influenza a virus, and adenoviruses ranged from 28 to 63%, but specificities for th ... | 1987 | 3029168 |
| early testing of cell cultures for detection of hemadsorbing viruses. | hemadsorption of primary monkey kidney cell cultures was commenced at 1 day after inoculation to evaluate how rapidly influenza a virus, influenza b virus, and parainfluenza virus types 1, 2, and 3 could be detected by this method from respiratory specimens. overall, 38% of all isolates could be detected within 24 h of inoculation, and 69% could be detected within 48 h. all influenza a viruses and all but one influenza b virus were detected by day 3. | 1987 | 3029173 |
| glycoproteins of human parainfluenza virus type 3: affinity purification, antigenic characterization and reconstitution into lipid vesicles. | monoclonal antibodies to the envelope glycoproteins, hn and f, of human parainfluenza virus type 3 were coupled to a sepharose 4b matrix and used for affinity purification of the viral glycoproteins. the purity of the glycoproteins was demonstrated by sds-page followed by fluorography or silver staining. the antigenicity of the glycoproteins was determined by immunization of rabbits; polyclonal rabbit antisera demonstrated inhibition of functional activities of the virus glycoproteins. the f gly ... | 1987 | 3029283 |
| diseases of wapiti utilizing cattle range in southwestern alberta. | specimens from 28 wapiti (cervus elaphus canadensis) were collected by hunters in southwestern alberta in 1984. various tests were performed to detect infections and conditions that could affect cattle sharing the range or cause disease in wapiti. serum antibodies were present against leptospiral serovars autumnalis (25%), bratislava (4%), and icterohaemorrhagiae (8%), and the viruses of bovine virus diarrhea (52%), infectious bovine rhinotracheitis (45%), and parainfluenza type 3 (13%). no sero ... | 1987 | 3029443 |
| cell fusion in viral diseases. | 1987 | 3029598 |