Publications

gal transduction by phage lambda: on the origin and nature of lft transducing genomes.		1970	4924698
a bacterial mutation which affects recognition of the n gene product of bacteriophage lambda.		1970	4924699
control of late messenger rna synthesis during lambda phage development.		1971	4926028
polar mutations in the left arm of bacteriophage lambda i434.	by studying complementation between frameshift and nonsense mutants located in the structural genes for the head of bacteriophage lambdai434, we found mutations in gene b which are polar on genes c and d and one mutation in gene e which is polar on gene f.	1971	4927522
the role of exonuclease and beta protein of phage lambda in genetic recombination. ii. substrate specificity and the mode of action of lambda exonuclease.		1971	4928646
protection of irradiated lambda phage by methylated proflavine.		1971	4930084
nucleotide sequence analysis of dna. ii. complete nucleotide sequence of the cohesive ends of bacteriophage lambda dna.		1971	4931680
repair of alkylation-induced damage in escherichia coli and its bacteriophage lambda.		1971	4932246
dna of vegetative bacteriophage lambda. vi. electron microscopic studies of replicating lambda dna.	electron micrographs of replicating lambda dna molecules, during the period of progeny-particle dna formation, show single-tailed circular structures. the tails are, predominantly, shorter than one viral genome.	1971	5276280
in vitro repression of rna synthesis by purified lambda phage repressor.		1971	5279042
fate of bacteriophage lambda dna after adsorption by haemophilus influenzae.		1971	5314514
regulation of leftward transcription in the j-b2-att region of coliphage lambda.		1971	5087422
light scattering and hydrodynamic properties of linear and circular bacteriophage lambda dna.		1971	5126521
the arrangement of dna in lambda phage heads. i. biological consequences of micrococcal nuclease attack on a portion of the chromosome exposed in tailless heads.		1971	5136580
the arrangement of dna in lambda phage heads. ii. lambda dna after exposure to micrococcal nuclease at the site of head-tail joining.		1971	5136581
regulation of gene expression in bacteriophage lambda.		1971	5138255
genetic localization of two polycistronic messenger rnas from bacteriophage lambda as deduced from their base compositions and annealing properties.		1971	5144630
temperature sensitivity of maltose utilization and lambda resistance in escherichia coli b.	escherichia coli b strains that have acquired the malb region from e. coli k-12 are able to utilize maltose and to adsorb phage lambda when grown at 30 c, but when grown at 40 c they do not absorb phage lambda and are devoid of amylomaltase activity. these mal(ts) lam(ts) cells can be mutated or transduced to become able to grow on maltose at 40 c, but they still have no detectable amylomaltase activity nor functional lambda receptors at that temperature. this mal(40) phenotype is governed by a ...	1971	4934064
dual control of arabinose genes on transducing phage lambda-dara.		1971	4934313
role of exonuclease and beta protein of phage lambda in genetic recombination. v. recombination of lambda dna in vitro.	the sequential action of lambda exonuclease and polynucleotide ligase upon redundant joint molecules is sufficient to produce intact polynucleotide chains and heat-stable, biologically active molecules of lambda dna, whereas the action of ligase alone is insufficient. these results (a) confirm the previously described mechanism of single-strand assimilation, including a subsidiary mechanism by which the further action of lambda exonuclease is arrested when a redundant strand is completely assimi ...	1971	4934524
ultraviolet- and x-ray-induced responses of a deoxyribonucleic acid polymerase-deficient mutant of escherichia coli.	escherichia coli k-12, polal(-) is a mutant strain whose extracts are deficient in kornberg deoxyribonucleic acid (dna) polymerase activity. we have compared the mutant and parental strains on the basis of a number of responses to ultraviolet (uv) and x-irradiation. for both types of radiation, the mutant is more sensitive by approximately the same factor as measured by reduction in colony formation, depression of dna synthesis, and enhancement of dna degradation. the rate of repair of x-ray-ind ...	1971	4935330
nucleotide sequence of a ribonucleic acid transcribed in vitro from lambda phage deoxyribonucleic acid.		1971	4936723
a phage lambda endonuclease controlled by genes o and p.		1971	4937245
purification and properties of 3-deoxy-d-arabinoheptulosonic acid-7-phosphate synthetase (phe) from a lambda arog+ transductant of escherichia coli.	by incorporating arog, the structural gene for 3-deoxy-d-arabinoheptulosonic acid-7-phosphate (dahp) synthetase (phe), into the genome of a heat-inducible susr60 mutant of phage lambda, it has been possible to increase the intracellular levels of dahp synthetase (phe) in a lysogenized strain of escherichia coli some 15-fold over levels found in the wild-type strain. by using this strain, the enzyme has been purified approximately 2,000-fold compared with wild type, and various kinetic parameters ...	1971	4937786
inactivation of escherichia coli, f' episomes at transfer, and bacteriophage lambda by psoralen plus 360-nm light: significance of deoxyribonucleic acid cross-links.	we have investigated some biological consequences of light-induced psoralen-deoxyribonucleic acid (dna) adducts and find that for several escherichia coli functions (killing of strain ab2480 reca13 uvra6, inactivation of phage lambda plaque-forming ability in wild type and uvra6 hosts, loss of ability to transmit intact flac(+) episomes), a light exposure sufficient for production of a single cross-link per dna molecule correlates well with the biological consequence. although one cross-link per ...	1971	4937788
lysogenization by bacteriophage lambda and the regulation of lambda repressor synthesis.		1971	4942211
sensitivity of intracellular bacteriophage lambda to colicin ca42-e2.	treatment of escherichia coli k-12 infected by lambda cits857 with colicin ca42-e2 resulted in partial inhibition of the infectious process. uninfected bacteria were killed by colicin with a probability of about five times that with which similarly treated lambda-infected bacteria lose plaque-forming ability. the lambda deoxyribonucleic acid (dna), when present in a bacterial cell either as the replicating dna of infectious phage or as the nonreplicating dna of superinfecting phage, was degraded ...	1971	4943074
bacteriophage p2: interaction with phage lambda and with recombination-deficient bacteria.		1971	4943192
bacterial mutants in which the gene n function of bacteriophage lambda is blocked have an altered rna polymerase.	bacterial mutants have been isolated, called gron, that block phage development by interference with the action of the product of the phage n gene. lambdatrp phages, which depend on the n product for the synthesis of tryptophan enzymes, do not make these enzymes in gron bacteria. two type of phage mutants have been isolated that can overcome the gron block. one type makes an altered n product, the other contains an n-bypass mutation. the gron mutation is closely linked to the rifamycin-resistanc ...	1971	4943550
establishment and maintenance of repression by bacteriophage lambda: the role of the ci, cii, and c3 proteins.	to define the events necessary for the establishment and maintenance of repression in a lambda-infected cell, we have studied the requirements for efficient synthesis of the ci protein ("lambda-repressor"). three classes of lambda mutants defective in the establishment of repression are also defective in the appearance of ci protein activity at the normal time. two of these mutational classes (cii(-) and ciii(-)) probably result from inactivation of lambda-specified proteins, but the third class ...	1971	4943791
the formation of homoimmune double lysogens of phage lambda and the segregation of single lysogens from them.		1971	4944853
[study of the lambda-exonuclease activity of escherichia coli during induction of the vegetative phage and infection with temperate phage lambda].		1971	4945393
a minor q-independent pathway for the expression of the late genes in bacteriophage lambda.		1971	4946855
[induction of lambda phage and transduction in bacterial forms with changed synthesis of the cell wall].		1971	4938667
specialized transduction of the salmonella hut operons by coliphage lambda: deletion analysis of the hut operons employing lambda-phut.		1971	4939450
[deletion and substitution of restriction sites in a hybrid phage lambda 80].		1971	4939843
control of leftward transcription in the immunity region of escherichia coli phage lambda.		1971	4940113
lysis defective mutants of bacteriophage lambda: genetics and physiology of s cistron mutants.		1971	4940968
interruption of dna injection by x-irradiation of phage lambda.		1971	5543269
lack of polarity of dna injection by escherichia coli phage lambda.		1971	5543271
the fine structures in melting curves of deoxyribonucleic acids of bacteriophage lambda. i.		1971	5543649
phenomenological theory of repair kinetics of ultraviolet irradiated lambda phage.		1971	5543830
uv-reactivation of bacteriophage lambda in excision repair-deficient hosts: independence of red functions and attachment regions.		1971	5543836
deletion mutants of bacteriophage lambda. ii. genetic properties of att-defective mutants.		1971	5551395
deletion mutants of bacteriophage lambda. 3. physical structure of att-phi.		1971	5551396
the role of exonuclease and beta protein of phage lambda in genetic recombination. 3. binding to deoxyribonucleic acid.		1971	5553408
cold-sensitive mutants of bacteriophage lambda.		1971	5556293
formation of concatemers of lambda phage dna in a recombination-deficient system.		1971	5563932
[a simple method of purifying bacteriophage lambda on wide pore glass].		1971	5567351
[transducing capacity of lambda-phage during disordered synthesis of the host bacterium cell wall].		1971	5571364
self-assembly of bacteriophage lambda tails.		1971	4106183
lysis defective mutants of bacteriophage lambda: on the role of the s function in lysis.		1971	4107551
breakdown of bacteriophage lambda dna in "escherichia coli" k12 "rec." bacteria.		1971	4107886
isolation and protein composition of normal and petit capsids of bacteriophage lambda.		1971	4108615
the in situ morphological transition of the bacteriophage lambda tubular head-forms.		1971	4109518
deletion mutants of bacteriophage lambda. i. isolation and initial characterization.		1971	4323931
genetic expression in bacteriophage lambda. iv. effects of p2 prophage on lambda inhibition of host synthesis and lambda gene expression.		1971	4331731
purification and base composition analysis of phage lambda early promoters.	rna-polymerase of escherichia coli was allowed to bind to dna of phage lambda in the absence of precursors. the resulting complex was excised by nuclease digestion and the protected dna was recovered by phenol-extraction and ethanol precipitation. acrylamide gel electrophoresis of protected dna fragments reveals the existence of two distinct oligonucleotide peaks corresponding, respectively, to 45-52 and 7-10 nucleotide residues along with species of intermediate sizes. peak i molecules have two ...	1971	4332016
genetic expression in bacteriophage lambda. iv. effects of p2 prophage on lambda inhibition of host synthesis and lambda gene expression.	the effects of p2 prophage on lambda-mediated depression of host metabolism, and on lambda macromolecular synthesis have been studied. experiments employing certain lambdabio deletion mutants and lambda-related phages suggest that the same gene products are involved in inhibition of host metabolism (hin+ function) and susceptibility to p2 inhibition (spi+ function). synthesis of early lambda mrna and protein occurs normally during the first 10 min after lambda infection of p2 lysogens, but subse ...	1971	18619370
amino-acid sequence of lambda phage endolysin.		1971	20480992
preferential binding of e. coli rna-polymerase to a-t rich sequences of bacteriophage lambda dna.		1971	11945591
the binding sites for escherichia coli rna polymerase on lambda phage dna: characteristics of binding sites on sonicated lambda dna.		1971	11945740
the binding sites for escherichia coli rna polymerase on lambda phage dna: various types of binding sites.		1972	11946581
discrimination by trna of two types of isolated binding sites for e. coli rna-polymerase on phage lambda dna.		1972	11946883
micrococcal nuclease treatment of bacteriophage heads alters the right-hand cohesive end of lambda deoxyribonucleic acid.	isolated bacteriophage lambda heads were exposed to micrococcal nuclease prior to addition of the phage tail. the deoxyribonucleic acid (dna) was extracted from the heads and sheared to half-molecules whose cohesive ends were annealed to normal lambda dna half-molecules. melting curves of each of the cohered halves indicated that only the right-hand termini are altered by nuclease treatment.	1972	16789135
establishment of repression by lambdoid phage in catabolite activator protein and adenylate cyclase mutants of escherichia coli.	lambdoid phages form clear plaques and show reduced ability to establish immunity in strains of escherichia coli that lack adenylate cyclase or catabolite activator protein. the absence of the activator protein or cyclic amp reduces the frequency of lysogenization, but does not prevent steady-state repressor synthesis of a lysogen. lambda phage mutants able to form turbid plaques on strains that lack catabolite activator protein or adenylate cyclase have been isolated and analyzed.	1972	4333980
requirement of adenosine 3',5'-cyclic phosphate for formation of the phage lambda receptor in escherichia coli.	adenosine 3',5'-cyclic phosphate is indispensable for formation of the phage lambda receptor in escherichia coli k-12.	1972	4334765
the role of adenosine 3',5'-cyclic monophosphate in the growth of bacteriophage lambda.		1972	4340810
biochemical method for inserting new genetic information into dna of simian virus 40: circular sv40 dna molecules containing lambda phage genes and the galactose operon of escherichia coli.	we have developed methods for covalently joining duplex dna molecules to one another and have used these techniques to construct circular dimers of sv40 dna and to insert a dna segment containing lambda phage genes and the galactose operon of e. coli into sv40 dna. the method involves: (a) converting circular sv40 dna to a linear form, (b) adding single-stranded homodeoxypolymeric extensions of defined composition and length to the 3' ends of one of the dna strands with the enzyme terminal deoxy ...	1972	4342968
synthesis of bacteriophage lambda proteins in vitro.		1972	4343946
dna nucleotide sequence restricted by the ri endonuclease.	the sequence of dna base pairs adjacent to the phosphodiester bonds cleaved by the ri restriction endonuclease in unmodified dna from coliphage lambda has been determined. the 5'-terminal nucleotide labeled with (32)p and oligonucleotides up to the heptamer were analyzed from a pancreatic dnase digest. the following sequence of nucleotides adjacent to the ri break made in lambda dna was deduced from these data and from the 3'-dinucleotide sequence and nearest-neighbor analysis obtained from repa ...	1972	4343974
morphogenesis of bacteriophage phi 80: identification of the cistron 13 product.	an in vitro complementation reaction leading to the assembly of bacteriophage phi80 tails from component proteins is described. tail assembly occurs when a lysate of any mutant in cistron 13 is mixed with a second lysate of a mutant in any of the other cistrons involved in tail formation. lysates of mutants that are blocked in tail formation contain phage heads that can unite with free tails to form infective particles. the rate of the complementation reaction shows little dependence upon temper ...	1972	4110106
the morphogenesis of phage lambda. v. form-determining function of the genes required for the assembly of the head.		1972	4113245
topology of dna and n gene expression in coliphage lambda.		1972	4503845
distance from a promoter mutation to an rna synthesis startpoint on bacteriophage lambda dna.		1972	4504486
bidirectional transcription and the regulation of phage lambda repressor synthesis.	there are two promoters for transcription of gene ci in phage lambda, the gene that codes for phage repressor. the promoters, called pre and prm, are located on the distal (pre) and proximal (prm) sides of gene cro, which itself is adjacent to ci. since ci and cro are transcribed in opposite directions, ci transcription initiating at pre gives rise to an antisense transcript of cro, while ci transcription initiating at prm does not. pre, active after infection of a sensitive cell, is stimulated ...	1972	4508309
an enhancing role for dna synthesis in formation of bacteriophage lambda recombinants.	recombination in some intervals of the map of phage lambda is associated with more dna synthesis than in other intervals. blockage of dna synthesis by high temperature in a host temperature-sensitive for dna synthesis results in the relative reduction of recombinant frequencies in those regions having the larger amounts of recombination-associated synthesis. reduction of dna synthesis at normal temperatures by a combination of the bacterial mutation and a mutation in one of the phage genes requi ...	1972	4509320
positive control of endolysin synthesis in vitro by the gene n protein of phage lambda.	positive control in vitro by gene n protein of bacteriophage lambda was demonstrated. lambda dna was used to direct in vitro synthesis of lambda endolysin in a cell-free protein-synthesizing preparation derived from escherichia coli. the endolysin synthesis depends on the concomitant in vitro synthesis of lambda gene n protein. when lambda n(-) dna was used to direct the cell-free preparation, endolysin was made only if extract was added from cells in which a lambda prophage had been induced. th ...	1972	4509321
growth of bacteriophage lambda in phospholipases a-less mutants.		1972	4551923
plasmid formation from bacteriophage lambda as a result of interference by resident plasmid lambda dv.		1972	4551991
lambda phage transcription in human fibroblasts.		1972	4551993
a mutation in escherichia coli enhancing the uv-mutability of phage lambda but not of its infectious dna in a spheroplast assay.		1972	4552500
plasmid formation from bacteriophage lambda in escherichia coli.		1972	4552709
studies on the dna photoreactivating enzyme from escherichia coli. i. transduction of the phr gene by bacteriophage lambda.		1972	4552790
kinetics of membrane association by bacteriophage lambda dna after repressor inactivation.		1972	4553104
large-scale production of lambda bacteriophage and purified lambda deoxyribonucleic acid.	large amounts of a heat-inducible phage lambda mutant (lambdaci857) may be obtained under standardized conditions. the phage is harvested by simple polyethylene glycol (c 6000) precipitation and purified by cscl density gradient banding. deoxyribonucleic acid (dna) is extracted by cold phenol and purified by sucrose density gradient sedimentation to yield a homogenous population of unbroken lambda dna molecules.	1972	4553145
studies on lysogeny in escherichia coli with bacteriophage lambda. physical observation of the insertion process.		1972	4553260
comparisons of the distribution of nucleotides and common sequences in deoxyribonucleic acid from selected bacteriophages.	results from comparisons of deoxyribonucleic acid (dna) from several classes of bacteriophages suggest that most phage chromosomes contain either a homogeneous distribution of nucleotides or are made up of a few, rather large segments of different quanine plus cytosine (g + c) contents which are internally homogeneous. among those temperate phages tested, most contained segmented dna. comparisons of sequence similarities among segments from lambdoid phage dna species revealed the following order ...	1972	4553679
effects of the rec and exr mutations of escherichia coli on uv reactivation of bacteriophage lambda damaged by different agents.		1972	4555297
uv reactivation of phage lambda in a pola mutant of e. coli.		1972	4555301
lytic replication of coliphage lambda in salmonella typhosa hybrids.	hybrids between escherichia coli k-12 and salmonella typhosa which conserved a continuous k-12 chromosomal diploid segment extending from pro through ara to the stra locus were sensitive to plaque formation by wild-type lambda. these partially diploid s. typhosa hybrids could be lysogenized with lambda and subsequently induced to produce infectious phage particles. when the k-12 genes were segregated from a lysogenic s. typhosa hybrid, phage-productive ability was no longer detectable due to los ...	1972	4555401
the morphogenesis of bacteriophage lambda. iv. identification of gene products and control of the expression of the morphogenetic information.		1972	4555611
host specificity of dna produced by escherichia coli. xvi. phage lambda dna carries a single site of affinity for a-specific restriction and modification.		1972	4555674
inactivation kinetics of lambda phage repressors in a mutant of e. coli temperature sensitive in dna replication.		1972	4555676
gene regulation in n mutants of bacteriophage lambda.	mutants (n(-)nin) of bacteriophage lambda in which the n gene product is not required for growth on wild-type escherichia coli do not plate on reca bacterial mutants. secondary mutants, selected for growth on reca, lie within the immunity region to the right of gene ci and appear identical to the cro mutants of eisen et al. in an n(+) phage, a cro mutation causes enhanced and prolonged production of lambda exonuclease. n(-)cro phages make no detectable exonuclease, but show an increased rate of ...	1972	4556510
rna synthesis startpoints in bacteriophage lambda: are the promoter and operator transcribed?		1972	4556643
induction of an endonuclease by some substitution and deletion variants of phage lambda.		1972	4556928
substrate specificity of the three endonucleases isolated from phage lambda c 1 -infected escherichia coli.		1972	4557025
recombination in bacteriophage lambda dna half molecules.		1972	4557198
isolation of plaque-forming, galactose-transducing strains of phage lambda.	plaque-forming, galactose-transducing lambda strains have been isolated from lysogens in which bacterial genes have been removed from between the galactose operon and the prophage by deletion mutation.-a second class has been isolated starting with a lysogenic strain which carries a deletion of the genes to the right of the galactose operon and part of the prophage. this strain was lysogenized with a second lambda phage to yield a lysogen from which galactose-transducing, plaque-forming phages w ...	1972	4558323