Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| avian adenovirus induces ion channels in model bilayer lipid membranes. | the action of duck egg drop syndrome 1976 (eds-76) adenovirus on model bilayer lipid membranes (blm) has been investigated on planar egg phosphatidylcholine membranes and small unilamellar vesicles. it was found that the adenovirus formed channels in planar blm in a ph-dependent manner. the addition of eds-76 to planar blm at ph 5 induced voltage-independent channel activity of about 60 ps conductivity after a lag phase. at ph 3, eds-76 induced irregular spikes of current across the planar blm w ... | 1997 | 9245727 |
| hydropathic characteristics of adenovirus hexons. | the complete nucleotide sequence and the predicted amino acid sequence of the adenovirus type 7 hexon gene were determined. the hydropathy of the hexon proteins from human adenovirus types 2, 3, 4, 5, 7, 12, 16, 40, 41, and 48, bovine adenovirus type 3, murine adenovirus type 1, and avian adenovirus types 1 and 10 was analysed. the presence of purines and pyrimidines in the second position of the codons was correlated to hydrophilicity and hydrophobicity, respectively. comparison of the hydrophi ... | 1997 | 9267445 |
| association of adenovirus infection with human obesity. | we previously reported that chickens infected with the avian adenovirus smam-1 developed a unique syndrome characterized by excessive intra-abdominal fat deposition accompanied by paradoxically low serum cholesterol and triglyceride levels. there have been no previous reports of avian adenoviruses infecting humans. we screened the serum of 52 humans with obesity in bombay, india, for antibodies against smam-1 virus using the agar gel precipitation test (agpt) method. bodyweights and serum choles ... | 1997 | 9385623 |
| purification of egg drop syndrome-76 virus by velocity density gradient centrifugation. a comparative study. | 1997 | 9607086 | |
| dna sequence analysis of an avian adenovirus terminal protein precursor. | nucleotide sequence of the genomic region between map units 25 and 31 of the fowl adenovirus serotype 10 (fav 10) was determined and analyzed. an open reading frame (orf) running from right to left (that is on /-strand) of 1806 nucleotides in length was found. this orf encoded a polypeptide of 602 amino acids with a molecular weight (m[r]) of approximately 70.4 kilo-daltons. the genomic location of the orf was determined to be between map units 25.5 and 29.5, similar to the genomic position of t ... | 1997 | 9311569 |
| close phylogenetic relationship between egg drop syndrome virus, bovine adenovirus serotype 7, and ovine adenovirus strain 287. | a cloned egg drop syndrome (eds) virus genomic dna fragment containing the protease gene has been identified and the complete nucleotide sequence of the protease and partial nucleotide sequence of the hexon genes has been determined. phylogenetic analysis of the protease gene has revealed eds virus to be genetically more closely related to bovine adenovirus type 7 (bav-7) and ovine adenovirus isolate 287 (oav287) than either of these two viruses are to other members of the genus mastadenovirus o ... | 1997 | 9123875 |
| isolation and identification of adenoviruses from ostriches (struthio camelus). | 1997 | 9141225 | |
| diagnosis of avian adenovirus infections using dna in situ hybridization. | three dna oligonucleotide probes designated fn-23, fn-48, and fn-96 were evaluated for the diagnosis of aviadenovirus infections by dna in situ hybridization. paraffin-embedded tissues were obtained from birds with confirmed adenovirus infection, birds with putative adenovirus infections, and birds with intranuclear inclusions caused by herpesvirus and polyomavirus. in birds with confirmed adenovirus infection, probes fn-23 and fn-96 identified 78% and 72% of diseased individuals, respectively. ... | 1997 | 9454909 |
| characterization of an avian adenovirus associated with inclusion body hepatitis in day-old turkeys. | a group i avian adenovirus isolated from day-old turkeys with inclusion body hepatitis (ibh) was identified as turkey adenovirus serotype 2 (tav2) based on cross-neutralization assays and dna restriction endonuclease analyses. yolk sac inoculation of embryonated turkey eggs resulted in embryo mortality and significantly (p < 0.01) decreased hatchability compared with sham-inoculated controls. embryo mortality occurred primarily between day 24 of incubation and the time embryos hatched. focal nec ... | 1997 | 9356722 |
| the complete nucleotide sequence of the egg drop syndrome virus: an intermediate between mastadenoviruses and aviadenoviruses. | the complete nucleotide sequence of an avian adenovirus, the egg drop syndrome (eds) virus, was determined. the total genome length is 33,213 nucleotides, resulting in a molecular weight of 21.9 x 10(6). the gc content is only 42.5%. between map units 3.5 and 76.9, the distribution of open reading frames with homology to known genes is similar to that reported for other mammalian and avian adenoviruses. however, no homologies to adenovirus genes such as e1a, pix, pv, and e3 could be found. outsi ... | 1997 | 9375018 |
| structural polypeptides of different clinical strains of avian adenovirus type-1. | the polypeptides of two liver strains, two ovarian strains and one standard strain of avian adenovirus-1 (aav-1) were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page), western blot using polyclonal antibodies to all the strains and isoelectric focusing (ief). all the strains had 11 polypeptides and molecular weight varied between 9 and 100 kda. the polypeptide pattern was similar in all the strains as revealed by sds-page and western blot. however, some differenc ... | 1997 | 9023041 |
| identification of a novel antiapoptotic protein, gam-1, encoded by the celo adenovirus. | we have developed a simple screening method to identify genes that mimic bcl-2 or adenovirus e1b 19k in enhancing cell survival after transfection and have used this method to identify such a gene in the avian adenovirus celo. the gene encodes a novel 30-kda nuclear protein, which we have named gam-1, that functions comparably to bcl-2 and adenovirus e1b 19k in blocking apoptosis. however, gam-1 has no sequence homology to bcl-2, e1b 19k, or any other known antiapoptotic proteins and thus define ... | 1997 | 9060680 |
| the oncogenic potential of eleven avian adenovirus strains. | oncogenic potentials of 11 strains of avian adenovirus representing 10 serotypes were investigated in 22 litters of 1-day-old syrian hamsters (mesocricetus auratus). hamsters were inoculated via two routes: subcutaneous dose of 0.12 ml and intracerebral dose of 0.02 ml. six strains, indiana-c (ind-c), chicken embryo lethal orphan (celo), stein, 75-1a, b-3, and a-2, induced fibrosarcomas subcutaneously at the inoculation site. hamsters inoculated intracerebrally with ind-c, celo, stein, b-3, and ... | 1997 | 9087343 |
| identification and characterization of penton base and pviii protein of egg drop syndrome virus. | the nucleotide sequence and location of the penton base and pviii genes of the egg drop syndrome (eds) virus an avian adenovirus, were determined. the penton base gene is located at 34.8-38.8 map units. the coding sequence has a length of 1359 nt and encodes a polypeptide of 452 amino acids (aa) with a molecular weight of 51 105 da. the amino acid sequence shows a homology of 57.3 and 55.3% to the structural protein ih of human adenovirus serotype 2 (had2) and fowl adenovirus serotype 1 (fav1). ... | 1997 | 9037737 |
| humoral immune response of chicks to different clinical isolates of avian adenovirus type-1. | the humoral immune response of 7-day-old chicks to different strains of avian adenovirus (aav) type-1 was determined by counter immunoelectrophoresis (cie) and enzyme-linked immunosorbent assay (elisa). in cie antibody was detected 14 days post-infection (pi) whereas in elisa antibody was detected 7 days pi. the elisa titre reached its peak at 21 days pi in all groups. the pattern of immune response among the groups was found to be similar. | 1997 | 9023042 |
| dna sequence analysis of the genes for the fowl adenovirus serotype 10 putative 33k and pviii. | the nucleotide sequence and genomic location of the fowl adenovirus serotype 10 (fav-10) putative 33k and precursor protein viii (pviii) genes have been determined. the total genomic region sequenced was 1814 base pairs (bp) in length with the 33k coding region occupying the sequence from nucleotides 44 to 634 and the pviii coding region nucleotides 949 to 1689. the location of both the 33k and pviii genes have the same positional organization as their human adenovirus (hav) counterparts which i ... | 1998 | 9773274 |
| inclusion body tracheitis associated with avian adenovirus in turkeys. | nine turkey flocks with basophilic intranuclear inclusion bodies, suggestive of adenovirus, within the epithelial cells of the tracheal mucosa were studied. respiratory signs and increased mortality occurred in turkeys between 6 and 10 wk of age from nine commercial turkey meat flocks in central california. necropsy findings included tracheitis and occasional mild sinusitis. histopathology of the tracheas revealed epithelial deciliation, squamous metaplasia, large basophilic intranuclear inclusi ... | 1998 | 9777161 |
| haematological and biochemical studies in broiler chicks fed ochratoxin and inoculated with inclusion body hepatitis virus, singly and in concurrence. | day-old broiler chicks, which had been shown to be negative for maternal antibodies against inclusion body hepatitis (ibh) virus and for viral antigen in cloacal swabs, were divided into four groups of 20 chicks each. one group was fed ochratoxin-a at 0.5 ppm from 3 to 38 days of age, another group was inoculated with 1 ml of ibh virus containing 10(6.5) eid50 per 0.2 ml. a third group was given both ochratoxin-a and infected with ibh virus. the fourth group served as the control. anaemia was ob ... | 1998 | 9778779 |
| sequence and transcriptional analysis of terminal regions of the fowl adenovirus type 8 genome. | fowl adenovirus (fadv) type 1, celo strain has no homologies to mastadenovirus e1a, e1b, e3 and e4, which regulate virus gene expression, dna replication and virus-host interaction. similarly, the right 5 kb and left 15 kb ends of celo virus dna are non-homologous to mastadenoviruses. to compare celo virus with another fadv, 7.5 kb of the left and 17 kb of the right ends of fadv type 8 (strain a-2a) were sequenced and nine and 17 open reading frames (orfs), respectively, were found. this fadv-8 ... | 1998 | 9780058 |
| [analysis of core virion polypeptides from the pathogen causing chicken egg-drop syndrome]. | the cores of egg-drop syndrome virus (eds-76) were isolated by the pyridine technique. eds-76 proved to be much more resistant to pyridine disruption than other adenoviruses and treatment with 10% pyridine did not lead to complete dissociation of capsid and cores; only increase of pyridine concentration to 20% produced satisfactory results. at least three polypeptides (24, 10.5, and 6.5 kda) were found in the core by sds-page, whereas the 40 kda reacting with the core is most probably not a core ... | 1998 | 9864829 |
| analysis of the hexon gene sequence of bovine adenovirus type 4 provides further support for a new adenovirus genus (atadenovirus). | the putative hexon gene of bovine adenovirus type 4 (bav-4), encoding 910 amino acid residues, has been identified and sequenced. a characteristic codon usage biased towards the use of at-rich triplets was observed. comparative analysis with other hexon sequences detected a high level of amino acid identity in the regions corresponding to the pedestals of the hexon. substitutions, insertions and deletions were identified mainly in the variable regions forming the loops which are exposed on the o ... | 1998 | 9634088 |
| [the serologic detection of virus-induced infections in the domestic goose (anser anser dom.)]. | the most important virus-induced diseases associated with heavy losses in the domestic goose are derzsy's disease which is caused by a goose parvovirus and duck plague (duck viral enteritis) which is caused by an avian herpesvirus. both diseases still occur but can be prevented by timely vaccinations. antibodies against influenza a viruses of the subtypes h1, h5, and h7 as well as against avian paramyxoviruses of the serogroups 4, 6, and 8, respectively, were not detected in any of the examined ... | 1998 | 9710927 |
| sequencing and phylogenetic analysis of the protease gene, and genetic mapping of bovine adenovirus type 10 define its relatedness to other bovine adenoviruses. | the complete genome of a bovine adenovirus (bav) type 10 isolate was molecularly cloned and partially sequenced. the encoded proteins were predicted by computer analysis of the dna sequences of the ends or the entire length of the cloned viral fragments, and thus a rough genetic map was constructed. the protease gene of bav-10 was completely sequenced and used in phylogenetic analysis. based on the results of the phylogenetic analysis, and the location and presence of certain genes thought to be ... | 1998 | 9712509 |
| the major late promoter and bipartite leader sequence of fowl adenovirus. | the region of the fowl adenovirus serotype 10 (fav-10) genome containing the major late promoter (mlp) and leader sequences was determined and appropriate genomic fragments were cloned and sequenced. a tata box was identified and the location of the putative transcription start site was determined. by using synthetic primers from the transcription start site in conjunction with oligonucleotides from the coding regions of the penton base and hexon genes, cdna was produced from late mrna isolated ... | 1998 | 9572553 |
| gastrointestinal pathogenicity of adenoviruses and reoviruses isolated from broiler chickens in alabama. | adenoviruses and reoviruses isolated from commercial broiler chickens were evaluated for gastrointestinal pathogenicity in specific-pathogen-free leghorn chickens. the viruses were originally isolated from either the proventriculus or a gastrointestinal pool of tissues of broiler chickens with proventriculitis or enteritis. isolates were cloned by terminal dilution. day-old chickens were inoculated by oral and ocular routes with undiluted tissue culture fluids (titers of 10[2]-10[4] tcid50/ml) a ... | 1998 | 9576341 |
| isolation of fowl adenoviruses serotype 4 from pigeons with hepatic necrosis. | nine homogenized livers were taken to isolate the causative agent of adenovirus type i and type ii infections in pigeons. the samples were passaged up to four times on primary chicken embryo hepatocytes. adenoviruses were isolated from all of the six type ii but none of the three type i infections. serologically the isolated adenoviruses were classified as fowl adenovirus (fav) serotype 4. restriction enzyme analysis of two isolates in comparison with fav4 reference strain kr5 confirmed the sero ... | 1998 | 9581348 |
| a proposal for a new (third) genus within the family adenoviridae. | this article presents a proposal for the establishment of a new adenovirus genus to accommodate certain bovine, ovine, and avian adenoviruses with special characteristics which differentiate them from members of the existing genera mastadenovirus and aviadenovirus. this proposal has been developed from earlier versions with advice from the adenovirus study group of the international committee on taxonomy of viruses (ictv). | 1998 | 9638153 |
| protection of broiler breeders by an inactivated combined water-in-oil-in-water viral vaccine. | a four-component vaccine, prepared by combining the single vaccines, contains subunits of newcastle disease and infectious bronchitis viruses, as well as whole inactivated infectious bursal disease and egg drop syndrome viruses. the vaccine is prepared in the form of a low-viscosity water-in-oil-in-water emulsion with low mineral oil content. heavy breeders were vaccinated at the age of 20 weeks by intramuscular administration of 0.5 ml vaccine/bird in an experiment carried out under field condi ... | 1998 | 9704508 |
| mode of inheritance of unselected traits in lines of chickens selected for high or low antibody response to sheep red blood cells. 1. resistance to marble spleen disease virus and juvenile body weight. | two lines of white leghorns that had undergone long-term selection for high (hh) or low (ll) antibody response to sheep red blood cell antigen(s) formed the nuclear lines for this experiment. matings were made in a full diallel cross to produce in a single hatch from age-contemporary breeders the parental lines, reciprocal f1 and f2 crosses, and backcrosses for 16 progeny types. for males and females, there were parental line differences in bw to 42 d of age, after which there was decline betwee ... | 1998 | 9706069 |
| growth characteristics of fowl adenovirus type 8 in a chicken hepatoma cell line. | fowl adenoviruses, many of which appear to be non-pathogenic, are ubiquitous in birds. in addition, the genome of these viruses is large, making them ideal candidates for construction as vectors for foreign genes. current methods to cultivate fowl adenoviruses use primary cell cultures derived from embryonated chicken eggs. in order to provide a more suitable culture method, the growth of fowl adenovirus type 8 (fadv-8) was investigated in ch-sah, a continuous hepatoma cell line. a one step grow ... | 1998 | 9763123 |
| transcriptional organization of the avian adenovirus celo. | a detailed map of the transcriptional organization of the celo virus genome was produced. recent computer analysis of celo virus has indicated the presence of 38 putative open reading frames (orfs). this study, based on analysis of the transcriptional products of celo in vitro, confirmed the presence of rnas for 26 of these 38 orfs. all of the results were obtained by cdna isolation or specific reverse transcriptase pcr. observation of orf transcription kinetics postinfection revealed the existe ... | 1998 | 9765475 |
| hexon based pcrs combined with restriction enzyme analysis for rapid detection and differentiation of fowl adenoviruses and egg drop syndrome virus. | three different polymerase chain reactions (pcrs), two of them combined with restriction enzyme analysis (rea), were developed for detection and differentiation of all 12 fowl adenovirus (fav) serotypes and the egg drop syndrome (eds) virus. for primer construction fav1, fav10 and eds virus hexon proteins were aligned and conserved and variable regions were determined. two primer sets (h1/h2 and h3/h4) for single use were constructed which hybridize in three conserved regions of hexon genes. eac ... | 1998 | 9766892 |
| serological survey of viral pathogens in bean and white-fronted geese from germany. | sera from wild geese were tested for antibodies to selected viral pathogens at a resting site for wild waterfowl in germany. serum samples from both bean geese (anser fabalis) and white-fronted geese (anser albifrons) collected in october 1991 were examined using serological methods licensed for routine diagnosis in domestic poultry. of 130 sera tested, antibodies to several infectious agents were found including newcastle disease virus (45%), goose parvovirus (48%), avian reovirus (29%), and av ... | 1998 | 9706557 |
| fowl adenovirus recombinant expressing vp2 of infectious bursal disease virus induces protective immunity against bursal disease. | the right hand end nde i fragment 3 (90.8-100 map units) of the fowl adenovirus serotype 10 (fav-10) was characterised so as to allow the location of an insertion site for recombinant vector construction. infectious bursal disease virus (ibdv) vp2 gene from the australian classical strain 002/73, under the control of the fav-10 major late promoter/leader sequence (mlp/ls) was inserted into a unique not i site that was generated at 99.5 map units. this recombinant virus was produced without delet ... | 1998 | 9645198 |
| [structural and functional organization of avian adenovirus eds-76 genome. sequencing and analysis of the 0-10.6% fragment of viral dna]. | 1998 | 9720064 | |
| the complete dna sequence and genome organization of the avian adenovirus, hemorrhagic enteritis virus. | hemorrhagic enteritis virus (hev) belongs to the adenoviridae family, a subgroup of adenoviruses (ads) that infect avian species. in this article, the complete dna sequence and the genome organization of the virus are described. the full-length of the genome was found to be 26,263 bp, shorter than the dna of any other ad described so far. the g + c content of the genome is 34.93%. there are short terminal repeats (39 bp), as described for other ads. genes were identified by comparison of the dna ... | 1998 | 9791022 |
| [adenovirus infections in psittacines]. | diagnostic investigations were carried out in a fatal disease of several african grey parrots (psittacus erithacus) and cape parrots (poicephalus robustus) in a large breeding plant. electron microscopically examination of liver and intestine, the organs with the most prominent pathomorphological changes, regularly revealed adenoviruses. necrotizing hepatitis and catarrhal to haemorrhagic enteritis dominated on histopathological examination. numerous basophilic intranuclear inclusion bodies in h ... | 1998 | 9931996 |
| dna sequence of the fowl adenovirus serotype 10 short fiber gene. | the complete nucleotide sequence and map location of the short fiber gene of fowl adenovirus serotype 10 (fav-10) strain cfa20 was determined. the coding sequence of the short fiber gene was found to be 1383 bp, encoding a putative polypeptide of 461 amino acids. the gene was located between 69.2 to 71.7 map units on the fav-10 genome. a consensus splice acceptor site was located 30 bp upstream of the putative translation start codon and a polya recognition sequence was located 24 bp downstream ... | 1998 | 10728824 |
| [early transcription of fav-1 (celo) avian adenovirus genome]. | the patterns of rna from cells infected with avian adenovirus chicken embryo lethal orphan (celo) virus were analyzed by northern hybridization method. early rnas are specifically hybridized with several fragments of the eco-ri restriction map: a (49-81%), b (0.2-20.3%), d (81-92.5%), and e fragments of the bam-hi celo dna restriction map. early rnas were not hybridized with c (37.8-49%), g (31-37.8%), and e (20.3-31%) fragments of the eco-ri dna celo restriction map. at least 19 types of virus- ... | 1998 | 10488533 |
| characterization of fowl adenoviruses from outbreaks of inclusion body hepatitis/hydropericardium syndrome in chile. | three fowl adenovirus (fav) isolates (341, 344, and 215) obtained during 1996-97 from field outbreaks of inclusion body hepatitis/hydropericardium syndrome (ibh/hps) affecting broilers and broiler breeders in chile were characterized by virus neutralization tests (vnts) and restriction enzyme analysis of a dna fragment. furthermore, the pathologic characteristics of one of these fav isolates (fav 341) was studied in experimentally infected chickens. the vnts conducted with isolates 341 and 344 a ... | 1999 | 10396639 |
| storage of viruses on filter paper for genetic analysis. | the purpose of this study was to develop a method to store viruses on filter paper without the need for special conditions for future use of the genetic material. two non-enveloped viruses were used as models. infectious bursal disease virus (ibdv), a double-stranded rna virus that infects chickens, belongs to the birnaviridae family. hemorrhagic enteritis virus (hev), with double-stranded dna, belongs to the adenoviridae family. three different solutions were found suitable for loading the viru ... | 1999 | 10598079 |
| response of turkeys to simultaneous vaccination with hemorrhagic enteritis and newcastle disease viruses. | the effects of single and combined vaccination of turkeys against hemorrhagic enteritis virus (hev) and newcastle disease virus (ndv) were investigated. dual vaccination of turkeys with ndv-b1 and hevp30 or marble spleen disease virus (msdv) enhanced white mottling of the spleens and the apoptosis rate in spleen cells (p < 0.05). in addition, simultaneously vaccinated turkeys had fewer hev-infected spleen cells at 4 days postvaccination than turkeys given hevp30 or msdv alone. the anti-hev antib ... | 1999 | 10396642 |
| csiro's 'natural' vaccines. | 1999 | 10561787 | |
| detection of avian adenovirus by polymerase chain reaction. | an avian adenovirus-specific polymerase chain reaction was developed. the origin of primers was from the dna sequence data of the chicken embryo lethal orphan avian adenovirus virus genome. an avian adenovirus-specific 421-bp dna product was amplified by these primers from group i of adenovirus containing 12 serotypes and serotypes of adenovirus from group ii and group iii. the adenovirus-specific dna product was also amplified from the 19 field isolates of avian adenoviruses but not from the ma ... | 1999 | 10216765 |
| characterization of celo virus proteins that modulate the prb/e2f pathway. | the avian adenovirus celo can, like the human adenoviruses, transform several mammalian cell types, yet it lacks sequence homology with the transforming, early regions of human adenoviruses. in an attempt to identify how celo virus activates the e2f-dependent gene expression important for s phase in the host cell, we have identified two celo virus open reading frames that cooperate in activating an e2f-inducible reporter system. the encoded proteins, gam-1 and orf22, were both found to interact ... | 1999 | 10400747 |
| [cloning and sequence analysis of 55 k protein of egg drop syndrome virus]. | for understanding the characteristics of genomic structure of egg drop syndrome virus(edsv). | 1999 | 12569783 |
| termination sequence requirements vary among genes transcribed by rna polymerase iii. | rna polymerase iii (pol iii) transcription generally terminates at a run of four or more thymidine (t) residues but some pol iii genes contain runs of t residues that are not recognized as termination signals. here, we investigate the terminal signal requirements that are operative in adenovirus virus-associated (va) rna genes. in the xenopus 5 s rna gene, efficient termination requires the t residues to be in a g+c-rich sequence context, but a run of five t residues in a g+c-rich context does n ... | 1999 | 10024448 |
| application of the polymerase chain reaction to detect fowl adenoviruses. | the possibility of using the polymerase chain reaction (pcr) for the detection of fowl adenoviruses (fadv) was tested. the optimal reaction parameters were evaluated and defined for purified genomic dna of type 8 fowl adenovirus (fadv-8), and then the same conditions were applied for nucleic acid extracted from infected cells. one hundred picograms of purified viral dna, or 250 fadv-8-infected cells, were detected by ethidium bromide staining of the pcr products in agarose gels. the sensitivity ... | 1999 | 10369570 |
| protection of turkeys from hemorrhagic enteritis with a recombinant fowl poxvirus expressing the native hexon of hemorrhagic enteritis virus. | hemorrhagic enteritis (he) is an economically important disease of turkeys caused by a type ii aviadenovirus, hemorrhagic enteritis virus (hev). the vaccines currently available to the commercial poultry producer are highly effective in preventing disease outbreaks; however, they are immunosuppressive. a recombinant fowl poxvirus (rfpv) expressing the native hexon of hev has been shown to induce an anti-hev humoral immune response in turkeys. in this study, the rfpv expressing the native hexon o ... | 1999 | 10396636 |
| mutational analysis of the avian adenovirus celo, which provides a basis for gene delivery vectors. | the avian adenovirus celo is being developed as a gene transfer tool. using homologous recombination in escherichia coli, the celo genome was screened for regions that could be deleted and would tolerate the insertion of a marker gene (luciferase or enhanced green fluorescent protein). for each mutant genome, the production of viable virus able to deliver the transgene to target cells was monitored. a series of mutants in the genome identified a set of open reading frames that could be deleted b ... | 1999 | 9882345 |
| [the patterns in the molecular biology of the adenoviruses (the results of a 20-year study at the institute of microbiology and virology of the national academy of sciences of ukraine)]. | scientific data of the highest importance and priority concerning regularities of structural and functional organization of proteins of adenoviruses capsids and peculiarities of expression of the virus genome are as follows: new antigen determinants of hexon and adenovirus fiber have been discovered, their different nature (conformational or linear) and different orientation, depending on the spatial organization of proteins, have been proved; localization of some epitopes has been determined wi ... | 1999 | 10330872 |
| pcr for specific detection of haemorrhagic enteritis virus of turkeys, an avian adenovirus. | a hexon gene based pcr was developed for specific amplification of dna sequences from the haemorrhagic enteritis virus (hev) of turkeys. the hexon genes of different avian adenoviruses were compared for primer construction. two regions with low sequence homology between hev and fowl adenovirus (fav) hexon genes were selected for primer localisation. in correlation with the known sequence data a fragment of 1647 bp was amplified from a live vaccine and spleens of turkeys suffering from haemorrhag ... | 1999 | 10488779 |
| dna sequence of frog adenovirus. | the genome of frog adenovirus (fradv-1) was sequenced and found to be the smallest of all known adenovirus genomes. the sequence obtained was 26163 bp in size and contains a substantial direct repeat near the right terminus, implying that it was derived by recombination from a parental genome of only 25517 bp. the closest relative of fradv-1 proved to be turkey adenovirus 3, an avian adenovirus with no previously known near relative. sequence comparisons showed that the two viruses have equivale ... | 2000 | 10993931 |
| pathogenic avian adenovirus type ii induces apoptosis in turkey spleen cells. | wild-type mammalian adenoviruses are known to inhibit programmed cells death in infected cells. this study demonstrated for the first time that an avian type ii adenovirus, the hemorrhagic enteritis virus (hev) of turkeys, induced apoptosis in turkey spleen cells at 3 and 4 days post infection. the increased apoptosis rate in spleens of hev-infected turkeys was associated with increased virus replication. increased apoptosis preceded extensive virus-induced cellular necrosis. at 3 days post infe ... | 2000 | 11003476 |
| serological monitoring on layer farms with specific pathogen-free chickens. | to monitor the existence of avian pathogens in laying chicken flocks, specific pathogen-free (spf) chickens were introduced into two layer farms and reared with laying hens for 12 months. spf chickens were bled several times after their introduction and examined for their sero-conversion to avian pathogens. as a result, antibodies to eight or ten kinds of pathogens were detected in spf chickens on each farm. antibodies to infectious bronchitis virus (ibv), avian nephritis virus, mycoplasma galli ... | 2000 | 11193353 |
| avian adenoviruses. | adenovirus infections are ubiquitous in commercially farmed birds, and probably in all avian species. there is a wide range of virulence, in some cases even within the same serotype. while many infections are subclinical and appear to be of little economic or welfare importance, significant outbreaks of disease associated with adenovirus do occur. these diseases are not of public health significance. | 2000 | 10935281 |
| the complete nucleotide sequence of fowl adenovirus type 8. | the fowl adenovirus type 8 (fadv-8) genome was sequenced and found to be 45063 nucleotides in length, the longest adenovirus (adv) genome for which the complete nucleotide sequence has been determined so far. no regions homologous to early regions 1, 3 and 4 (e1, e3 and e4) of mastadenoviruses were recognized. gene homologues for early region 2 (e2) proteins, intermediate protein iva2 and late proteins were found by their similarities to protein sequences from other advs. however, sequences homo ... | 2000 | 10859390 |
| chicken anemia virus and fowl adenoviruses: association to induce the inclusion body hepatitis/ hydropericardium syndrome. | the effects of a simultaneous and/or a subsequent coinfection with chicken anemia virus (cav) isolate 10343 and fowl adenovirus (fav) isolate 341 in specific-pathogen-free light chickens were evaluated. the simultaneous coinfection was conducted by the intramuscular route, whereas the subsequent coinfection trial considered favs administered orally. in trial 1, 20-day-old chickens simultaneously coinfected with cav (10343) and fav (341) intramuscularly (i.m.) showed 55% mortality and characteris ... | 2000 | 10737644 |
| the inhibitory effect of the imidazoquinolinamine s-28828 on the pathogenesis of a type ii adenovirus in turkeys. | in this study we show that a type i-ifn inducing compound, s-28828, modulated the pathogenesis of an avian type ii adenovirus in turkeys. by itself, s-28828 induced a strong reaction in the spleen characterized by hyperplasia of the red and white pulps as well as an increase in lymphoid cell aggregations. oral administration of s-28828 before the time of virus inoculation suppressed significantly (p<0.05) the replication of hemorrhagic enteritis virus (hev) in turkeys. two doses of 5 or 50 mg of ... | 2000 | 10867157 |
| [epizootiology of fowls adenoviruses]. | fowl adenoviruses of the serotype 4 from germany were characterised by restriction enzyme analysis in comparison to isolates from asia, south america and the fav4 reference strain kr5. only strain da60 which was isolated from a psittacine aviary was identical with the reference strain kr5. none of the isolates was identical with the highly pathogenic strains from india and ecuador. one-day-old chicks were infected orally and intramuscularly with the reference strain kr5, the psittacine isolate d ... | 2000 | 10846813 |
| an ovine adenovirus vector lacks transforming ability in cells that are transformed by ad5 e1a/b sequences. | adenoviruses of the mastadenovirus and aviadenovirus genera are able to transform certain cell types and induce tumor formation in susceptible animals. for the mastadenoviruses the e1a/b sequences are largely responsible for these properties but e4 sequences may also be involved. the transforming sequences of the aviadenoviruses, which lack e1a/b and e4 homologues, have not yet been fully identified. the recent proposal for a third genus of adenoviruses, which apparently lack an e1a homologue an ... | 2000 | 10772988 |
| the hydropericardium syndrome and inclusion body hepatitis in domestic fowl. | hydropericardium syndrome, an emerging disease of poultry, has recently been detected in some countries of asia and america, particularly in broiler birds aged 3-6 weeks. the disease is characterized by its sudden occurrence with high mortality of up to 80% in broilers and low mortality of under 10% in layers, associated with hydropericardium. its course is of 7-15 days under natural conditions. the causative agent is probably fowl adenovirus serotype 4, belonging to group i aviadenovirus genus ... | 2000 | 10726299 |
| activation of heat-shock response by an adenovirus is essential for virus replication. | successful viral infection requires viruses to redirect host biochemistry to replicate the viral genome, and produce and assemble progeny virions. cellular heat-shock responses, which are characterized as elevation and relocalization of heat-shock proteins, occur during replication of many viruses. such responses might be host reactions to the synthesis of foreign protein, or might be irrelevant consequences of the viral need to activate transcription. alternatively, as heat-shock proteins can f ... | 2000 | 11001061 |
| delivery of avian cytokines by adenovirus vectors. | a fowl adenovirus serotype 8 (fav-8) recombinant was constructed by inserting an expression cassette consisting of the fav major late promoter/splice leader sequences (mlp/sl), the chicken interferon-gamma (chifn-gamma) gene and sv40 polya into sites in the right hand end of the fav-8 genome. one recombinant (a3-13) was constructed by an insertion of chifn-gamma into a 1.3 kilobase pair (kbp) deletion which removed a putative open reading frame (orf) with identity to the celo (fav serotype 1) 36 ... | 2000 | 10717297 |
| induction of hydropericardium in one-day-old specific-pathogen-free chicks by adenoviruses from inclusion body hepatitis. | the pathogenicities of inclusion body hepatitis (ibh) and hydropericardium syndrome (hps) strains of adenovirus for specific-pathogen-free (spf) chicks were compared. one-day-old spf chicks inoculated intramuscularly with the dpi-2 (serotype 2), s-pl1 (serotype 2), tr630 (serotype 8), and saga97 (serotype 8) strains from ibh and with the lvp-1 strain (serotype 4) from hps exhibited the mortality, liver enlargement, and hydropericardium characteristic of gross change found in hps. the chicks inoc ... | 2000 | 10737661 |
| [adenovirus kr95, isolated from chickens during an outbreak of hydropericarditis, is the pathogen of this disease]. | virus agent kr95 was isolated from the liver of dieoff chickens during an outbreak of hydropericarditis syndrome at a poultry farm in russia. electron microscopic examination of the virus morphology, comparative restriction cleavage map construction, dna-dna hybridization, and analysis of structural proteins from purified and disrupted virions showed that the agent is to be classified as type 1 avian adenovirus. | 2000 | 10867994 |
| increased adiposity in animals due to a human virus. | four animal models of virus-induced obesity including adiposity induced by an avian adenovirus have been described previously. this is the first report of adiposity induced in animals by a human virus. | 2000 | 10951537 |
| the orf rtl1 transcript of fowl adenovirus type-8 is spliced and truncated at late stages of the virus replication cycle. | two transcription products were found for the open reading frame (orf) rtl1 located near the right terminus of the fowl adenovirus type-8 genome. the larger transcript, which was transcribed mostly during the early stage of the virus infection, contains the complete sequence (933 nucleotides) of the predicted orf from the genomic dna sequence encoding a 311 amino acid (aa) polypeptide. in contrast, the shorter transcript, which was more predominant at the late stage of the infection, was missing ... | 2000 | 10872874 |
| pathogenicity and gene analysis of adenovirus from pigeons with inclusion body hepatitis. | the pathogenicity of an adenovirus isolated from pigeons (pigeon adenovirus, pa) with inclusion body hepatitis in taiwan was investigated in specific-pathogen-free (spf) chicks and in racing pigeons. one-day-old spf chicks were inoculated subcutaneously with 10(4) 50% tissue culture infective dose (tcid50, low dose) and 10(8) tcid50 (high dose) of the virus, respectively. the chicks began to die three days post inoculation (dpi) with high dose of the virus and the mortality reached 100%; the chi ... | 2000 | 11039596 |
| purification and differentiation of three avian adenoviruses by restriction endonuclease analysis. | a rapid method of ultracentrifugation pelleting of avian adenovirus (aav) from small volume of chloroform treated infected cell culture fluid or allantoic fluid was adapted for isolation of adenoviral dna. the viral dna extracted from semipurified viruses was found to be intact on agarose gel and pure enough (a260/280 = 1.85-1.92) for restriction enzyme analysis. restriction endonuclease analysis of indian strain of aav serotype 1, aav serotype 4 (group i aavs) and egg drop syndrome-76 (eds-76) ... | 2000 | 11218838 |
| vertical induction of the inclusion body hepatitis/hydropericardium syndrome with fowl adenovirus and chicken anemia virus. | the hypothesis that fowl adenovirus (fav) and chicken anemia virus (cav), transmitted vertically and simultaneously, induce the inclusion body hepatitis (ibh)/hydropericardium (hp) syndrome in progeny chickens was tested. thus, 35-wk-old light brown layer breeders, showing absence of antibodies against fav and variable titers against cav, were intramuscularly singly infected with the fav serotype 4 isolate 341 or dually infected with cav (isolate 10343) and fav. all hens (groups a [fav alone], b ... | 2001 | 11336070 |
| ultrastructural changes in the liver of birds experimentally infected with the agent of hydropericariium syndrome. | 2001 | 11719983 | |
| nucleotide sequence of l1 and part of p1 of hexon gene of fowl adenovirus associated with hydropericardium hepatitis syndrome differs with the corresponding region of other fowl adenoviruses. | in order to study the serotypic variations in hydropericardium hepatitis syndrome (hhs) causing virus, the dna was extracted from the purified virus, a 0.7 kb variable region of hexon gene encoding l1 and part of p1 amplified and sequenced. both nucleotide and derived amino acid sequences, corresponding to the variable region, were compared with the published fowl adenovirus sequences (fav serotypes 10, 1 and 8). as expected the 0.7 kb sequence showed single open reading frame (orf). there was a ... | 2001 | 11118737 |
| the long repeat region is dispensable for fowl adenovirus replication in vitro. | two regions containing tandemly repeated sequences are present in the fowl adenovirus 9 (fadv-9) genome. the longer repeat region (tr-2) is composed of 13 contiguous 135-bp-long direct repeats, the function of which is unknown. an infectious fadv-9 genomic clone, constructed by homologous recombination in escherichia coli, was used for engineering of recombinant viruses. the enhanced green fluorescence protein (egfp) coding sequence was cloned in both rightward and leftward orientations so as to ... | 2001 | 11336545 |
| defining car as a cellular receptor for the avian adenovirus celo using a genetic analysis of the two viral fibre proteins. | the coxsackievirus and adenovirus receptor (car) is a high affinity receptor used by adenoviruses, including adenovirus type 5 (ad5). the adenovirus fibre molecule bears the high affinity cell binding domain of ad5, allowing virions to attach to car. the avian adenovirus celo displays two fibre molecules on its capsid and it was logical to expect that the cell binding functions of celo might also reside in one or both of these fibres. we had previously shown that the cell binding properties of c ... | 2001 | 11369892 |
| marble spleen disease in pheasants in korea. | two pheasants maintained in outdoor closed pen died within several days after having a history of depression. on necropsy, the spleens from both pheasants were enlarged about 3 times of their normal size and appeared mottled in color varying white to red. histopathologically, there were diffuse severe follicular necrosis in the spleen and congestion and edema in the lung. intranuclear basophilic inclusion bodies, which are strongly positive to group ii avian adenovirus with immunohistochemistry, ... | 2001 | 11459022 |
| construction of avian adenovirus celo recombinants in cosmids. | the avian adenovirus celo is a promising vector for gene transfer applications. in order to study this potentiality, we developed an improved method for construction of adenovirus vectors in cosmids that was used to engineer the celo genome. for all the recombinant viruses constructed by this method, the ability to produce infectious particles and the stability of the genome were evaluated in a chicken hepatocarcinoma cell line (lmh cell line). our aim was to develop a replication-competent vect ... | 2001 | 11333910 |
| adenovirus-mediated expression of a truncated pdgfbeta receptor inhibits thrombosis and neointima formation in an avian arterial injury model. | platelet-derived growth factor (pdgf) is a major mediator of neointima formation after arterial injury. we constructed a recombinant adenovirus, ad/pdgftr, that expresses the soluble extracellular domain of the murine pdgfbeta receptor (pdgftr). the expressed pdgftr was appropriately glycosylated and secreted by chicken vascular smooth muscle cells (smcs) in vitro. the expressed pdgftr inhibited human pdgf-bb induced receptor autophosphorylation, and also inhibited pdgf-bb induced cell prolifera ... | 2001 | 11583327 |
| development of sandwich elisa for the detection of fowl adenovirus 4 associated with hydropericardium syndrome in experimentally infected chicken. | a sandwich elisa was standardized to detect fowl adenovirus (fav) group i antigen in various tissues, namely liver, spleen, bursa, thymus and kidneys of chicks experimentally infected with fowl adenovirus 4 (fav-4) isolated from cases of inclusion body hepatitis-hydropericardium syndrome (ibh-hps). the assay was found to be more sensitive and more specific in comparison to an agar gel immunodiffusion (agid) test, as it could detect fav antigen below the titer of 20,000 tcid50/ml and below 1.14 m ... | 2001 | 11719988 |
| proliferation of lung macrophages in acute fatal viral infections in chickens. | marked proliferation of macrophages engulfing yellow pigments and fragmented erythrocytes were seen in the air capillaries and blood capillaries of the lungs of chickens affected with acute fatal viral hydropericardium syndrome, highly pathogenic infectious bursal disease, and highly pathogenic avian influenza. proliferation of lung macrophages was associated with systemic proliferation of macrophages. acute destruction of erythrocytes in these infections may have induced systemic hyperplasia of ... | 2001 | 11785886 |
| detection and confirmation of reptilian adenovirus infection by in situ hybridization. | adenovirus infections are documented in at least 12 different species of reptiles. in contrast to their mammalian and avian counterparts reptilian adenoviruses are not well characterized as to their pathogenic potential and their ability to cause primary disease. in the diagnostic setting, fresh tissues are often not available for virus isolation, and the confirmation of reptilian adenovirus infections is dependent largely upon electron microscopy for the identification of intranuclear viral inc ... | 2001 | 11478615 |
| gizzard erosion in broiler chicks by group i avian adenovirus. | incidences of mortality without any clinical signs occurred in growing chicks in a broiler flock. five 17-day-old dead broilers with gizzard erosions were investigated pathologically. macroscopically, the gizzards were found to be dilated with bloody fluids. the koilin layer of the gizzards showed multifocal black patches (erosions). histologically, there was necrosis of the koilin layer, degeneration and depletion of the gizzard glandular epithelium with intranuclear inclusion bodies, and hyper ... | 2001 | 11332488 |
| epizootic outbreaks of gizzard erosion associated with adenovirus infection in chickens. | two outbreaks of gizzard erosion in slaughtered broiler chickens in japan were examined pathologically and microbiologically. the prevalences of such lesions were 9%-11% and 4%-50% in the affected flocks. affected chickens had no clinical signs. group i fowl adenovirus (fav) serotype 1 was isolated from gizzard lesions. histologically, gizzard mucosa were necrotic. intranuclear inclusion bodies were seen in the enlarged nuclei of degenerating epithelial cells of the gizzard. the keratinoid layer ... | 2001 | 11332495 |
| experimental infection of specific-pathogen-free chickens with serotype-1 fowl adenovirus isolated from a broiler chicken with gizzard erosions. | gizzard lesions were formed in specific-pathogen-free (spf) white leghorn chickens inoculated with fowl adenovirus (fav). the virus, serotype 1 fav 99zh strain (fav-99zh), was originally isolated from the gizzard mucosa of commercial broiler chickens exhibiting gizzard erosion with intranuclear inclusion bodies. five-day-old and 53-day-old spf white leghorn chickens were inoculated with fav-99zh by both oral and ocular routes and then examined at necropsy on days 3, 5, 7, 10, 14, and 21 postinoc ... | 2001 | 11332481 |
| unique features of fowl adenovirus 9 gene transcription. | we examined the transcriptional organization of fowl adenovirus 9 (fadv-9) and analyzed temporal transcription profiles of its early and late mrnas. at least six early and six late transcriptional regions were identified for fadv-9. extensive splicing was observed in all fadv-9 early transcripts examined. sequence analysis of the cdnas representing the early proteins identified untranslated leader sequences, precise locations of splice donor and acceptor sites, as well as polyadenylation signals ... | 2002 | 12441071 |
| histone deacetylase 1 inactivation by an adenovirus early gene product. | gam1 is an early gene product of the avian adenovirus celo and is essential for viral replication. gam1 has no homology to any known proteins; however, its early expression and nuclear localization suggest that the protein functions to influence transcription in the infected cell. a determinant of eukaryotic gene expression is the acetylation state of chromosomal histones and other nuclear proteins. we find that gam1 expression increases the level of transcription from a variety of eukaryotic pr ... | 2002 | 11937030 |
| prevention of inclusion body hepatitis/hydropericardium syndrome in progeny chickens by vaccination of breeders with fowl adenovirus and chicken anemia virus. | the hypothesis that an effective protection of progeny chickens against inclusion body hepatitis/hydropericardium syndrome (ibh/hp) can be achieved by dual vaccination of breeders with fowl adenovirus (fav) serotype 4 and chicken anemia virus (cav) was tested. thus, 17-wk-old brown leghorn pullet groups were vaccinated by different schemes including single fav (inactivated), single cav (attenuated), fav and cav dually, or were not vaccinated (controls). subsequent progenies of these breeders wer ... | 2002 | 12243517 |
| the use of pcr combined with restriction enzyme analysis to characterize fowl adenovirus field isolates from northern india. | ten fowl adenoviruses (favs), isolated from suspected cases of inclusion body hepatitis (ibh) in quails and broilers, were characterized by a hexon-based polymerase chain reaction (pcr) combined with restriction enzyme analysis (rea) of the amplified dna fragments. all the isolates could be detected using h1/h2 and h3/h4 primer sets. amplification of dna with h1/h2 and h3/h4 primer sets resulted in fragments of approximately 1219 bp and 1319 bp, respectively. haeii digestion of the h1/ h2 pcr pr ... | 2002 | 12416873 |
| purification and characterization of the aetiological agent of hydropericardium hepatitis syndrome from infected liver tissues of broiler chickens. | hydropericardium hepatitis syndrome in broiler chickens is an acute, infectious disease characterized by high mortality, excess pericardial fluid and multifocal hepatic necrosis. the aetiological agent was purified to homogeneity from infected liver tissues from field outbreaks. electron-microscopic and serological confirmation of the virus were undertaken and the disease was reproduced experimentally in broiler chicks. the results indicated that an adenovirus, fowl adenovirus serotype 4, was al ... | 2002 | 11887423 |
| a fiber gene-based polymerase chain reaction for specific detection of pigeon adenovirus. | a polymerase chain reaction (pcr) was developed to detect a recently described pigeon adenovirus (piav). primers located in the fiber gene of piav amplified a pcr fragment solely from piav dna, whereas all 12 serotypes of fowl adenoviruses (fav1 to fav12), some of them able to infect pigeons, did not react. a pcr fragment of 967 base pairs was amplified from three different isolates serologically typed as piav and from some pigeon liver samples showing morphological and histological signs of an ... | 2002 | 12425796 |
| [eukaryotic vectors of celo avian adenovirus genome, carrying gfp and human il-2 genes]. | recombinant celo avian adenoviruses carrying green fluorescent protein (gfp) and and human interleukin-2 (il-2) genes were obtained by homologous recombination in cell culture. the resultant recombinant celo viruses are reproduced in chick embryos in the renal tubular and chorionic allantoic membrane cells. the ability of celo vectors to transduce human and animal cells was studied in vitro (in cell cultures) and in vivo (in laboratory animals). gfp gene delivery and expression in recombinant ce ... | 2002 | 12180023 |
| pancreatic necrosis and ventricular erosion in adenovirus-associated hydropericardium syndrome of broilers. | seven 19-day-old broiler chickens affected with hydropericardium syndrome (hps) with pancreatic necrosis and gizzard erosions were investigated pathologically and virologically. mortality increased after 13 days of age in a flock on a broiler farm. the mortality rate of the flock reached 10% by 19 days of age. macroscopically, the chickens had hydropericardium (the characteristic gross change of hps), pinpoint white foci in the pancreas, and ventricular erosions. histologically, the chickens had ... | 2002 | 12014508 |
| reduced efficacy of hemorrhagic enteritis virus vaccine in turkeys exposed to avian pneumovirus. | avian pneumovirus (apv) is an immunosuppressive respiratory pathogen of turkeys. we examined the effect of apv infection on the vaccine efficacy of hemorrhagic enteritis virus (hev) vaccines. apv was inoculated in 2-wk-old turkeys. two or four days later, an attenuated hev vaccine (hevp30) or marble spleen disease virus (msdv) vaccine were administered. virulent hev challenge was given 19 days after hev vaccination. apv exposure compromised the ability of hevp30 and msdv to protect turkeys again ... | 2002 | 12061644 |
| fowl adenovirus serotype 4 associated with outbreaks of infectious hydropericardium in haryana, india. | during 1998, hydropericardium syndrome was observed among 3-to-6-wk-old broilers in 45 different flocks of haryana, india, with mortality ranging between 10% and 30%. fowl adenovirus (fav) was isolated from one of the affected flocks by chicken embryo liver cell culture. serum neutralization test and polymerase chain reaction assay coupled with restriction enzyme analysis confirmed that the isolated virus belonged to fav serotype 4. the disease was reproduced in 28-day-old broilers by subcutaneo ... | 2002 | 11922341 |
| antibodies to adenoviruses in free-living common buzzards from germany. | one hundred sixty-seven plasma samples of free-living birds of prey from berlin and brandenburg state (germany) were tested for antibodies against avian adenovirus (fav, group i) using agar gel precipitation test. antibodies to fav were detected in seven (4%) of 167 total samples. the positive samples originated only from common buzzards (buteo buteo; seven [12%] of 59). this serologic survey provides evidence of natural exposure of free-living common buzzards from eastern germany to adenoviruse ... | 2002 | 12238387 |
| experimental gizzard erosions in specific-pathogen-free chicks by serotype 1 group i avian adenoviruses from broilers. | the pathogenicity of serotype 1 group i avian adenovirus (giaav) strains isolated from gizzard erosions for 1-wk-old specific-pathogen-free (spf) chickens was investigated. in experiment 1, spf chickens inoculated orally with a serotype 1 strain (tokushima2000/ge) of giaav isolated from gizzard erosion in broilers were euthanatized and necropsied from 3 to 12 days after inoculation. clinically, there were no significant signs in the chickens inoculated with tokushima strain. the gizzards were fo ... | 2002 | 12495050 |
| induction of short interspersed nuclear repeat-containing transcripts in epithelial cells upon infection with a chicken adenovirus. | chicken embryo lethal orphan adenovirus (celo) is used as a vector for expression of exogenous genes in mammalian cells. here, we analyzed transcriptional alterations in mouse epithelial host cells following infection with celo using cdna microarray analysis. sequence data characterization revealed that a major portion of celo-induced genes contained short interspersed nuclear elements of the b2 subclass (b2 sines). in fact, we could identify sines and other repetitive sequences as contributing ... | 2003 | 12729754 |
| preliminary data of adenovirus celo localisation in chicken. | 2003 | 14965138 | |
| detection of egg drop syndrome 1976 virus by polymerase chain reaction and study of its persistence in experimentally infected layer birds. | polymerase chain reaction (pcr) assay was developed for the detection of egg drop syndrome 1976 (eds-76) virus in tissues, namely in the uterus, spleen and buffy coat. it was also used to study the persistence of the virus in tissues of experimentally infected layer birds. the pcr assay could detect as little as 10 fg of purified eds-76 viral dna. it also amplified the dna of fowl adenovirus serotypes 4 (fav-4) and 8 (fav-8). the virus persisted in the uterus up to day 21 post infection (p.i.). ... | 2003 | 14658847 |
| hematology, plasma biochemistry, and serosurvey for selected infectious agents in southern giant petrels from patagonia, argentina. | in conjunction with reproductive and feeding ecology studies on southern giant petrels (sgp, macronectes giganteus) blood samples were collected for baseline health evaluations. twenty-five adult sgp from a breeding colony in chubut, argentina, were sampled during two consecutive breeding seasons, 1999-2000 (n = 15) and 2000-01 (n = 10). values for hematology, plasma biochemistry, and minerals are described for 20 birds in apparent good physical condition. a serologic survey of exposure to selec ... | 2003 | 12910764 |