Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| immunoglobulin d (igd) of atlantic cod has a unique structure. | a new immunoglobulin heavy-chain gene with some homology to mammalian igd was recently cloned from the channel catfish and atlantic salmon, two species of teleost fish. we have cloned and sequenced a new h-chain gene from atlantic cod (gadus morhua l.) which has clear similarities to these genes, but which also differs in several ways. the similarities of catfish, salmon, and cod delta to the mammalian delta genes are sequence homology, location immediately downstream of igm (mu), and expression ... | 2000 | 10866112 |
| environment and sex determination in farmed fish. | a plasticity of gonadal sex differentiation was reported in the 1930s following exogenous steroid treatments in fish, but demonstration that environmental factors (temperature, ph, density and social interactions) could influence the sex ratio in gonochoristic species has been relatively recent. in fish, as in reptiles and amphibians displaying environmental sex determination, the main environmental factor influencing sex seems to be temperature (tsd=temperature sex determination). in most therm ... | 2001 | 11738628 |
| channel catfish follicle-stimulating hormone and luteinizing hormone: complementary dna cloning and expression during ovulation. | gonadotropins are important regulators of reproduction. to develop molecular resources for production of recombinant gonadotropins, we have cloned and sequenced complementary dna encoding the channel catfish (ictalurus punctatus) follicle-stimulating hormone (fsh) and luteinizing hormone (lh), which encode 132 and 140 amino acid proteins, respectively. the deduced amino acid sequences of the catfish fsh and lh are highly conserved with those cloned from other teleosts. both the fsh and the lh we ... | 2001 | 14961331 |
| characterisation of saprolegnia sp. isolates from channel catfish. | nineteen channel catfish isolates of saprolegnia sp. obtained from 5 separate fish farms in mississippi, which became affected by winter kill syndrome during 1991 and 1996, were investigated with respect to physiological characteristics and genetic variation. isolates of s. parasitica from crayfish and s. diclina were included for comparison. most strains of catfish isolates grew well at 20 and 30 degrees c. repeated zoospore emergence was found in catfish isolates of saprolegnia sp. and s. para ... | 2001 | 11411644 |
| complete nucleotide sequence of the cytochrome b gene of channel catfish ictalurus punctatus and comparison of sequence homology among channel catfish and other fishes. | to clarify the phylogenic relationship of channel catfish with other fishes, the cytochrome b (cyt b) gene of the catfish was cloned and sequenced. channel catfish (ictalurus punctatus) belonging to the family ictaluridae in the order siluriformes showed a 78.4-87.4% similarity to all but one fish of the family cyprinidae and river loach crossostoma lacustre of the family balitoridae in the order cypriniformes in which genes had already been sequenced, and a 97.2% similarity to the goldfish (car ... | 2001 | 11258463 |
| the effects of tributyltin (tbt) and 3,3',4,4',5-pentachlorobiphenyl (pcb-126) mixtures on antibody responses and phagocyte oxidative burst activity in channel catfish, ictalurus punctatus. | the organotin tributyltin (tbt) is an antifouling biocide used in marine paints and is a common pollutant in harbor estuaries. we previously demonstrated that the immune system of channel catfish, ictalurus punctatus, is a sensitive target organ of tbt. exposure strongly suppresses humoral immune responses. harbor estuaries often contain polychlorinated biphenyls (pcbs) due to their ubuquitous distribution. the coplanar congener 3,3',4,4'5'-polychlorinated biphenyl (pcb-126) is also immunotoxic ... | 2001 | 11443370 |
| molecular biology of channel catfish gonadotropin receptors: 1. cloning of a functional luteinizing hormone receptor and preovulatory induction of gene expression. | there is little known about the molecular biology of piscine gonadotropin receptors, and information about gene expression during reproductive development is particularly lacking. we have cloned the lh receptor (lhr) in the channel catfish (cc), and examined its gene expression throughout a reproductive cycle. a cdna encoding the receptor was isolated from the testis using reverse transcription-polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends procedures. it encoded a 696-a ... | 2001 | 11207219 |
| microsatellite-containing genes from the channel catfish brain: evidence of trinucleotide repeat expansion in the coding region of nucleotide excision repair gene rad23b. | expansion of trinucleotide repeats within genes is well known to cause pathological conditions in humans. here we report a large number of genes containing simple sequence repeats (ssr) from the brain of channel catfish, of which a homologue of the rad23b gene was found to include (cca) trinucleotide repeats within its coding region. because of the importance of the rad23b gene in the nucleotide excision repair (ner) system, the catfish rad23b locus was further characterized. the (acc) repeats e ... | 2001 | 11716474 |
| genetic manipulations in aquaculture: a review of stock improvement by classical and modern technologies. | the aim of this review was to highlight the extent to which the genetic technologies are implemented by the aquaculture industry. the review shows that some of the modern genetic technologies are already extensively applied by the diverse aquaculture industries, though not to the same extent for all important aquacultured species (according to fao 1998 figures). some species (common carp, atlantic salmon, rainbow trout, channel catfish, nile tilapia, and the pacific oyster) received concentrated ... | 2001 | 11841164 |
| molecular biology of the channel catfish gonadotropin receptors: 2. complementary dna cloning, functional expression, and seasonal gene expression of the follicle-stimulating hormone receptor. | molecular cloning of the channel catfish fsh receptor is reported together with temporal changes in the gene expression throughout a reproductive cycle. a cdna encoding the receptor was isolated from the testis using reverse transcription-polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends (race) procedures. the cdna coded for a 662-amino acid protein that was most identical (51%-59%) to salmon gonadotropin receptor i and the fsh receptors of higher vertebrates, and less iden ... | 2001 | 11514332 |
| multiple isoforms and an unusual cathodic isoform of creatine kinase from channel catfish (ictalurus punctatus). | in vertebrates, the creatine kinase (ck) family consists of two cytosolic and two mitochondrial isoforms. the two cytosolic isoforms are the muscle type (m-ck) and the brain type (b-ck). here we report multiple ck isoenzymes in the diploid channel catfish (ictalurus punctatus) with one unusual cathodic isoform that was previously found only in pathological situations in human. the cathodic ck isoform existed only in the channel catfish stomach, ovary, and spleen, but not in any other species ana ... | 2001 | 11587847 |
| thioredoxin acts as a b cell growth factor in channel catfish. | to identify differentially expressed genes from channel catfish macrophages, a cdna library from lps-stimulated catfish macrophages was screened by subtractive hybridization. this screening yielded a 552-bp cdna coding for catfish thioredoxin (cf-trx). the deduced amino acid sequence revealed that cf-trx contains 107 amino acids and is 59% homologous to human adult t cell leukemia-derived factor/trx, originally described as an il-2r alpha-inducing factor. northern blot analyses showed that cf-tr ... | 2001 | 11207242 |
| prevalence of streptococcus iniae in tilapia, hybrid striped bass, and channel catfish on commercial fish farms in the united states. | to determine the prevalence of streptococcus iniae in tilapia (oreochromis spp), hybrid striped bass (morone chrysops x m saxatilis), and channel catfish (ictalurus punctatus) on commercial fish farms in the united states. | 2001 | 11212023 |
| occurrence of ichthyophthirius multifiliis within the peritoneal cavities of infected channel catfish ictalurus punctatus. | ichthyophthirius multifiliis is a ciliated protozoan parasite that infects the skin and gills of freshwater fish. this report describes the unusual finding of i. multifiliis within the peritoneal cavities of experimentally infected channel catfish ictalurus punctatus. twenty catfish fingerlings were exposed to i. multifiliis theronts using a standardized protocol. five infected fish and 2 control fish were killed at various time points after infection and their tissues examined. formalin-fixed, ... | 2001 | 11253873 |
| no detectable dna excision repair in uv-exposed hepatocytes from two catfish species. | dna repair is a critical process in protecting cellular genetic information from mutation. nucleotide excision repair (ner) is a mechanism by which cells correct dna damage caused by agents that form bulky covalent adducts and uv photoproducts such as thymine dimers and 6-4 photoproduct. ner, sometimes called dark repair, is generally accepted as being low in fish compared to mammals. this study was designed to quantitate ner in two related catfish species that have known differential sensitivit ... | 2001 | 11255108 |
| accumulation, distribution, and toxicity of copper in sediments of catfish ponds receiving periodic copper sulfate applications. | copper sulfate (cuso4) is applied periodically to commercial channel catfish (ictalurus panctatus) ponds as an algicide or parasiticide. current understanding of the chemistry of copper in soil-water systems suggests that copper may accumulate in pond sediments, although the forms and potential bioavailability of copper in catfish pond sediments are not known. this study investigated the accumulation and distribution of copper in the sediment of catfish ponds receiving periodic additions of cuso ... | 2001 | 11401280 |
| histone-like protein: a novel method for measuring stress in fish. | we assessed the effect of chronic stress using a group of potent, broad-spectrum antimicrobial polypeptides, called histone-like proteins (hlps), which appear to be an important component of non-specific immunity in channel catfish ictalurus punctatus skin. an enzyme-linked immunosorbent assay (elisa) was developed to measure the predominant hlp (hlp-1) in channel catfish skin. catfish were then exposed to a chronic stress consisting of overcrowding and elevated ammonia. healthy unstressed fish ... | 2001 | 11324821 |
| effect of lectins on the invasion of ichthyophthirius theront to channel catfish tissue. | this study determined the effects of lectin binding to theronts of ichthyophthirius multifiliis on theront immobilization, invasion, trophont development and survival in channel catfish ictalurus punctatus excised fins in vitro. soybean agglutinin (sba), lentil agglutinin (lca), gorse agglutinin (uea-i) and wheat germ agglutinin (wga) were used to treat theronts. percentages of theronts immobilized by 4 lectins ranged from 12.0 to 19.4% at a concentration of 1000 microg ml(-1). these lectins bou ... | 2001 | 11463098 |
| hormonal control of osmoregulation in the channel catfish ictalurus punctatus. | prolactin (prl) is an important hormone for freshwater adaptation in many teleost species. in some euryhaline fishes, growth hormone (gh) and cortisol are involved in seawater adaptation by stimulating ion extrusion. when channel catfish (ictalurus punctatus) were transferred from fresh water to dilute seawater (300-400 mosm), their plasma osmolality was always higher than the environmental salinity. in correlation with the increase in plasma osmolality, significant increases in plasma cortisol ... | 2001 | 11356039 |
| portals of entry and systemic localization of proliferative gill disease organisms in channel catfish ictalurus punctatus. | immunized rabbit serum adsorbed in live catfish was used in indirect fluorescent antibody test (ifat) to detect developmental life stages of henneguya ictaluri n. sp. this myxozoan parasite is associated with proliferative gill disease in channel catfish ictalurus punctatus (rafinesque) in the usa. specific pathogen free fingerlings were experimentally infected with the actinosporean stage of h. ictaluri and necropsied 24, 48, 72, and 96 h post-infection. at 24 h post-infection parasite stages w ... | 2001 | 11843138 |
| inactivation of frog virus 3 and channel catfish virus by esculentin-2p and ranatuerin-2p, two antimicrobial peptides isolated from frog skin. | while it is clear that some amphibian populations have recently experienced precipitous declines, the causes of those die-offs are complex and likely involve multiple variables. one theory suggests that environmental factors may trigger events that result in depressed immune function and increased susceptibility to infectious disease. here we examine one aspect of innate immunity in amphibians and show that esculentin-2p (e2p) and ranatuerin-2p (r2p), two antimicrobial peptides isolated from ran ... | 2001 | 11601906 |
| molecular cloning and functional characterization of a novel delayed rectifier potassium channel from channel catfish (ictalurus punctatus): expression in taste buds. | the gustatory system of channel catfish is widely studied for its sensitivity to amino acids. as a first step in identifying the molecular components that play a role in taste transduction in catfish, we cloned the full-length cdna for kv2-catfish, a novel k(+) channel that is expressed in taste buds. the deduced amino acid sequence is 816 residues, and shares a 56-59% sequence identity with kv2.1 and kv2.2, the other members of the vertebrate kv2 subfamily of voltage-gated k(+) channels. the kv ... | 2001 | 11238731 |
| enhanced bacterial disease resistance of transgenic channel catfish ictalurus punctatus possessing cecropin genes. | the cecropin b gene from the moth hyalophora cecropia, driven by the cytomegalovirus promoter, was transferred to the channel catfish ictalurus punctatus. transgenic individuals (p1) were mated to produce individuals (f1) that exhibited enhanced disease resistance and survival when challenged with pathogenic bacteria. during the epizootic of flavobacterium columnare in an earthen pond, the percentage of transgenic individuals containing preprocecropin b construct that survived (100%) was signifi ... | 2002 | 14961267 |
| the use of synthetic genes for the expression of ciliate proteins in heterologous systems. | the common fish parasite, ichthyophthirius multifiliis, expresses abundant glycosylated phosphatidylinositol (gpi)-anchored membrane proteins known as immobilization antigens, or i-antigens. these proteins are targets of the host immune response, and have been identified as potential candidates for recombinant subunit vaccine development. nevertheless, because ichthyophthirius utilizes a non-standard genetic code, expression of the corresponding gene products, either as subunit antigens in conve ... | 2002 | 12034497 |
| bolbophorus damnificus n. sp. (digenea: bolbophoridae) from the channel catfish ictalurus punctatus and american white pelican pelecanus erythrorhynchos in the usa based on life-cycle and molecular data. | the common pathogenic prodiplostomulum metacercaria in the flesh, mostly near the skin, of pond-produced channel catfish ictalurus punctatus has been demonstrated to be bolbophorus damnificus overstreet & curran n. sp. the catfish acquires the infection from the snail planorbella trivolvis, the only known first intermediate host, and the species is perpetuated through the american white pelican pelecanus erythrorhynchos, as confirmed by experimental infections with nestling and dewormed adult pe ... | 2002 | 12075128 |
| oct2 transcription factor of a teleost fish: activation domains and function from an enhancer. | oct2 transcription factors of the catfish (ictalurus punctatus) are expressed as alternatively spliced alpha and beta isoforms. functional analysis revealed an n-terminal glutamine (q)-rich transactivation domain common to both isoforms of catfish oct2. a c-terminal proline, serine, threonine (pst)-rich activation domain was identified exclusively in the beta isoform. activation domains of fish and mammalian oct2 showed cell type- and species-specific activity correlated with their biochemical c ... | 2002 | 12127069 |
| isolation and molecular characterization of herpesvirus from cultured european eels anguilla anguilla in taiwan. | a herpesvirus has been isolated for the first time from a population of european eels anguilla anguilla cultured in a recirculated system in taiwan. syncytia formation was detected in ep-1 (eel epidermis) cell cultures inoculated with cell-free homogenates prepared from both integument and visceral organs of moribund fish. inoculation of homogenates onto ek (eel kidney) cell cultures induced giant cell formation. subsequent passages produced a consistent and progressive cytopathic effect (cpe) i ... | 2002 | 12180701 |
| health of lake whitefish (coregonus clupeaformis) with elevated tissue levels of environmental contaminants. | lake whitefish (coregonus clupeaformis) were collected in 1996 from the st. lawrence river, quebec, canada. histologic examination was performed on major organs of 497 specimens and on the liver of 48 additional individuals. preneoplastic and neoplastic hepatic changes consisted of vacuolated cell (n = 65), clear cell (n = 17), and acidophilic (n = 16) foci of altered hepatocyte, hepatocellular carcinoma (n = 12), cholangioma (n = 5), and cholangiocarcinoma (n = 28). six fish were intersexes (1. ... | 2002 | 11883413 |
| circulating lysozyme and hepatic cyp1a activities during a chronic dietary exposure to tributyltin (tbt) and 3,3',4,4',5-pentachlorobiphenyl (pcb-126) mixtures in channel catfish, ictalurus punctatus. | exposure to planar congeners of halogenated aromatic hydrocarbons (hahs) leads to a myriad of toxicities, including developmental, reproductive, and immunotoxic effects. halogenated aromatic hydrocarbons possessing structural similarity to tcdd, such as 3,3',4,4',5-pentachlorobiphenyl (pcb-126), are more prevalent in the environment than tcdd, and they elicit similar toxic effects, primarily through the cytosolic aromatic hydrocarbon receptor (ahr). while polychlorinated biphenyls (pcbs) are ubi ... | 2002 | 11991632 |
| cloning and characterization of edwardsiella ictaluri proteins expressed and recognized by the channel catfish ictalurus punctatus immune response during infection. | an edwardsiella ictaluri expression library was screened for clones expressing antigenic e. ictaluri proteins using anti-e. ictaluri serum, which resulted in the isolation of 32 clones. the clones were partially characterized and 4 were selected for complete analysis. sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page), 2-dimensional page, western blotting, and dna sequencing were used to analyze expressed antigenic proteins and encoded genes. sequence analysis identified 4 puta ... | 2002 | 12542086 |
| bioaccumulation of pcbs in aquatic biota from a tidal freshwater marsh ecosystem. | water, sediments, and aquatic biota were sampled in a tidal river-marsh on the potomac river near washington, dc (usa) to assess baseline concentrations of polychlorinated biphenyls (pcbs) and bioaccumulation in finish species. the mean sediment total-pcb concentration in the wetland was 50 ng/g dry weight, and mean concentrations in biota ranged from 150 ng/g to 450 ng/g wet weight. the highest pcb concentrations were observed in channel catfish. the median biota-sediment accumulation factor (b ... | 2002 | 11994779 |
| first record of neoergasilus japonicus (poecilostomatoida: ergasilidae), a parasitic copepod new to the laurentian great lakes. | the parasitic copepod neoergasilus japonicus, native to eastern asia, was first collected from 4 species of fish (fathead minnow, pimephales promelas; largemouth bass, micropterus salmoides; pumpkinseed sunfish, lepomis gibbosus; and yellow perch, perca flavescens) in july 1994 in saginaw bay, lake huron, michigan. further sampling in the bay in 2001 revealed infections on 7 additional species (bluegill, lepomis macrochirus; carp, cyprinus carpio; channel catfish, ictalurus punctatus; goldfish, ... | 2002 | 12197110 |
| mercury concentrations in fish from lake mead, usa, related to fish size, condition, trophic level, location, and consumption risk. | total mercury (hg) concentrations were determined in the skeletal muscle of 339 fish collected during the fall of 1998 and spring of 1999 from lake mead, usa, the nation's largest human-made reservoir. five species of fish representing a range of trophic levels and the lake's principal game fishes were studied. hg generally increased with trophic level and fish size. median hg concentrations (ng/g wet mass) were 277 in striped bass, 168 in channel catfish, 160 in largemouth bass, 75 in bluegill, ... | 2002 | 12202927 |
| evaluation of estrogenic activity from a municipal wastewater treatment plant with predominantly domestic input. | the purpose of this study was to survey estrogenic releases from two primarily domestic wastewater treatment plants over three seasons (1996-1999). mature male channel catfish were maintained at two sites within each wwtp and a reference site for 21 days. estrogenic activity of effluent was assessed by the yeast estrogen screen (yes) assay (in 1999) and the expression of the female egg yolk precursor protein, vitellogenin (vtg) in caged male channel catfish (1996-1998). serum vtg of animals expo ... | 2002 | 12359391 |
| immunisation of channel catfish, ictalurus punctatus, with ichthyophthirius multifiliis immobilisation antigens elicits serotype-specific protection. | surface immobilisation antigens (i-antigens) were purified from two strains of ichthyophthirius multifiliis (ny1 and g5) that represent different i-antigen serotypes, namely a and d, respectively. the efficacy of the purified antigens as subunit vaccines was then tested in challenge studies using parasites of the homologous or heterologous serotype. three groups of juvenile channel catfish (70 animals per group) were immunised with i-antigens from either the g5 or ny1 isolates, or with bovine se ... | 2002 | 12458741 |
| edwardsiella ictaluri invasion of iec-6, henle 407, fathead minnow and channel catfish enteric epithelial cells. | invasion of edwardsiella ictaluri into cultured mammalian, fish and enzymatically harvested catfish enteric epithelial cells is described. gentamicin survival assays were used to demonstrate the ability of this catfish pathogen to invade iec-6 (origin: rat small intestinal epithelium), henle 407 (origin: human embryonic intestinal epithelium), fathead minnow (fhm, minnow epithelial cells) and trypsin/pepsin-harvested channel catfish enteric epithelial cells. invasion of all cell types occurred w ... | 2002 | 12465874 |
| factors controlling the bioavailability of ingested methylmercury to channel catfish and atlantic sturgeon. | the bioavailability of ingested methylmercury (ch3hg(iii) was investigated in vitro using the gastric and intestinal fluids of channel catfish, ictalurus punctatus, and atlantic sturgeon, acipenser oxyrinchus. gastric fluid collected from each species was incubated with ch3hg(ii)-spiked sediment or bloodworms, after which the intestinal fluid of each species was added and incubated further. the proportion of ch3hg(ii) solubilized from bloodworms and sediment appeared to be controlled by complexa ... | 2002 | 12523429 |
| protective immunity induced by dna vaccination of channel catfish with early and late transcripts of the channel catfish herpesvirus (ihv-1). | seven full-length transcripts encoding four early and three late genes of the channel catfish virus (ccv), ictalurid herpesvirus i (ihv-1), have been cloned following rt-pcr amplification and dna sequencing. transcripts were selected based on their predicted association with membrane structures, identification as an envelope glycoprotein, or as a viral capsid protein. the transcripts derived from orf 6, orf 7, orf 8a, orf 10, orf 51, orf 53, and orf 59 were all shown to be complete and unspliced ... | 2002 | 11777531 |
| initial characteristics of koi herpesvirus and development of a polymerase chain reaction assay to detect the virus in koi, cyprinus carpio koi. | since 1998, episodes of mass mortality have occurred in populations of common carp cyprinus carpio carpio in israel and in populations of koi cyprinus carpio koi in israel and the usa. a herpesvirus isolated from infected fish has been shown in experimental studies to induce disease and mortality similar to those observed in outbreaks at infected farms. initial characteristics of the virus show that it is clearly different from herpesvirus cyprini (chv), the most commonly known herpesvirus from ... | 2002 | 12005231 |
| surface immobilization antigen of the parasitic ciliate ichthyophthirius multifiliis elicits protective immunity in channel catfish (ictalurus punctatus). | channel catfish (ictalurus punctatus) that survive infection with the parasitic ciliate ichthyophthirius multifiliis acquire immunity to subsequent challenge and produce specific antibodies in serum that immobilize the parasite in vitro. cellular surface protein antigens targeted by these antibodies are referred to as immobilization antigens (i-antigens). by using an immobilizing mouse monoclonal antibody as a ligand, the i-antigen of i. multifiliis isolate g5 was purified to homogeneity by immu ... | 2002 | 11777850 |
| fluorescein: a rapid, sensitive, nonlethal method for detecting skin ulceration in fish. | there is a need to develop simple, rapid, and accurate methods for assessing health in fish populations. in this study we demonstrate that use of fluorescein, a nontoxic fluorescent dye, can rapidly and easily detect the presence of skin ulcers in all fish tested, including rainbow trout (oncorhynchus mykiss), channel catfish (ictalurus punctatus), goldfish (carassius auratus), and hybrid striped bass (morone saxatilis male x m. chrysops female). exposure of fish to as little as 0.10 mg fluoresc ... | 2002 | 12450204 |
| sequence and expression of androgen receptor and estrogen receptor gene in the sex types of protogynous wrasse, halichoeres trimaculatus. | sex steroid hormones play important roles in sex change and behavior of wrasses. their actions are considered to be mediated through the nuclear hormone receptors. in this study, to elucidate the roles of estrogen receptor (er) and androgen receptor (ar) in the reproduction of the protogynous wrasse, halichoeres trimaculatus, ar and er genes were partially cloned using 5'- and 3'-race and their transcript levels in the gonads and the brain were measured by competitive rt-pcr. the amino acid sequ ... | 2002 | 12383444 |
| bioavailability and toxicity of dietborne copper and zinc to fish. | to date, most researchers have used dietborne metal concentrations rather than daily doses to define metal exposure and this has resulted in contradictory data within and between fish species. it has also resulted in the impression that high concentrations of dietborne cu and zn (e.g. > 900 mg kg(-1) dry diet) are relatively non-toxic to fish. we re-analyzed existing data using rations and dietborne metal concentrations and used daily dose, species and life stage to define the toxicity of dietbo ... | 2002 | 12161165 |
| inhibition of erythrocyte delta-aminolevulinic acid dehydratase (alad) activity in fish from waters affected by lead smelters. | we assessed the effects on fish of lead (pb) released to streams by smelters located in trail, bc (canada), e. helena, mt, herculaneum, mo, and glover, mo. fish were collected by electrofishing from sites located downstream of smelters and from reference sites. blood from each fish was analyzed for delta-aminolevulinic acid dehydratase (alad) activity and hemoglobin (hb), and samples of blood, liver, or carcass were analyzed for pb, zinc (zn), or both. fish collected downstream of all four smelt ... | 2002 | 12139078 |
| molecular identification and expression analysis of tumor necrosis factor in channel catfish (ictalurus punctatus). | a tumor necrosis factor (tnf) alpha-like gene, encoding a propeptide of 230 amino acids and a mature (soluble) peptide of 162 amino acids, was identified in channel catfish (ictalurus punctatus). while the catfish protein shared features in common with both mammalian tnfalpha and tnfbeta homologs, overall sequence identity/similarity was slightly higher vs. tnfalpha genes when mature tnf sequences were compared. phylogenetic analysis placed catfish and other fish tnf sequences within their own c ... | 2003 | 12880635 |
| cloning and sequencing of cytochrome p450 2x1 from channel catfish (ictalurus punctatus). | previous purification and immunochemical studies in livers of channel catfish indicated the presence of at least four cytochrome p450 (cyp) 2-like isoforms. sequencing of the first 18 amino acids of one purified form indicated a cyp2 isoform. from this n-terminal sequence and other published cyp2 sequences from fish, primers were designed and a full-length cyp cdna was identified from reverse-transcribed catfish liver mrna. 5' and 3' race was used to obtain an open reading frame of 1470 bp encod ... | 2003 | 12408592 |
| spinitectus macrospinosus n. sp. (nematoda: cystidicolidae) from the channel catfish ictalurus punctatus in southern manitoba and its distribution in other ictalurus spp. | spinitectus macrospinosus n. sp., a parasite of the channel catfish ictalurus punctatus, from the red and assiniboine rivers in southern manitoba, canada, is characterized by spines reaching a maximum length of 21.8-26.8 microm (means for males and females, respectively) in the anterior esophageal region, arranged in 4 sectors in the anterior region of the body. spines in this region of the body increase in size and decrease in number (from a maximum of 6 to 3 per sector). the combination of spi ... | 2003 | 14533691 |
| characterization of cdna encoding immunoglobulin light chain of the mandarin fish (siniperca chuatsi). | immunoglobulin light chain cdna sequences of a perciform fish, the mandarin fish siniperca chuatsi were amplified from head kidney mrna by reverse transcription (rt)-pcr and race methods using degenerated primer and gene specific ones. in cdna sequences of the vl region, nucleotide exchanges were present mainly within cdrs, although a lesser degree of variability was also found in frs. moreover, the length of cdr1 and cdr3 in the mandarin fish is shorter than in most other fish species. in the m ... | 2003 | 12969639 |
| conservation of a glutathione s-transferase in marine and freshwater fish. | we have previously reported the isolation and cloning of glutathione s-transferase (gst) cdnas from two marine fish, english sole (pleuronectes vetulus) and starry flounder (platichthys stellatus), that exhibited > 95% identity to plaice (pleuronectes platessa) gst-a aquatic toxicol., 44, 171-182]. in the present study, we have used reverse transcription-polymerase chain reaction (rt-pcr) analysis to isolate a 471 nucleotide gst-like cdna from largemouth bass (micropterus salmoides) liver. seque ... | 2003 | 11460686 |
| fish tissue quality in the lower mississippi river and health risks from fish consumption. | between 1990 and 1994, samples of three shellfish species (i.e. blue crab, callinectes sapidus;crayfish, procambarus acutis; and river shrimp, macrobrachium ohionii) and 16 fish species and were collected at six sites along the lower mississippi river by the louisiana department of environmental quality, office of water resources in coordination with the us environmental protection agency. the fish species included: bigmouth buffalo (ictiobus cyanellus); blue catfish (ictalurus furcatus); carp ( ... | 2003 | 12526903 |
| systemic herpes-like virus in catfish ictalurus melas (italy) differs from ictalurid herpesvirus 1 (north america). | a herpesvirus was isolated during 2 occurrences of mass mortality among adult catfish ictalurus melas raised in different farms in northern italy. the agent replicated in the channel catfish ovary (cco) cell line from channel catfish i. punctatus, inducing a cytopathic effect similar to that caused by ictalurid herpesvirus 1 (also referred to as channel catfish herpesvirus, ccv). the new herpesvirus, designated i. melas herpesvirus (icmhv) did not react with polyclonal rabbit or monoclonal antib ... | 2003 | 12911055 |
| pathogen loads, clearance and plasma cortisol response in channel catfish, ictalurus punctatus (rafinesque), following challenge with edwardsiella ictaluri. | 2003 | 12946014 | |
| development of an enzyme-linked immunosorbent assay for the measurement of plasma growth hormone (gh) levels in channel catfish (ictalurus punctatus): assessment of environmental salinity and gh secretogogues on plasma gh levels. | we report the development of a sensitive, and specific, competitive, antigen-capture enzyme-linked immunosorbent assay for the measurement of channel catfish (ictalurus punctatus) growth hormone (cfgh). the detection limit of the assay (90% binding) was 2.0ng/ml and the ed(50) value (standard curve range 150-0.59 ng/ml) was 67.3 ng/ml. recovery of cfgh-spiked plasma samples was determined to be 102%. dose-response inhibition curves using serially diluted pituitary homogenates and plasma samples ... | 2003 | 12957475 |
| novel derivatives of 9,10-anthraquinone are selective algicides against the musty-odor cyanobacterium oscillatoria perornata. | musty "off-flavor" in pond-cultured channel catfish (ictalurus punctatus) costs the catfish production industry in the united states at least 30 million us dollars annually. the cyanobacterium oscillatoria perornata (skuja) is credited with being the major cause of musty off-flavor in farm-raised catfish in mississippi. the herbicides diuron and copper sulfate, currently used by catfish producers as algicides to help mitigate musty off-flavor problems, have several drawbacks, including broad-spe ... | 2003 | 12957919 |
| protective effect of cutaneous antibody produced by channel catfish, ictalurus punctatus (rafinesque), immune to ichthyophthirius multifiliis fouquet on cohabited non-immune catfish. | fish which survive a sublethal ichthyophthiriasis acquire protective immunity against ichthyophthirius multifiliis fouquet (ich). this study evaluated the protective effect of cutaneous antibody secreted by channel catfish, ictalurus punctatus (rafinesque), immune to ich on cohabited non-immune catfish. non-immune and immune fish controls were separately maintained and infected with theronts. the ich infection was assessed by scoring 0, < 50, 50-100, and > 100 trophonts fish(-1) at 5 days post-i ... | 2003 | 12962237 |
| cloning and gene expression of p450 17alpha-hydroxylase,17,20-lyase cdna in the gonads and brain of the fathead minnow pimephales promelas. | p450 17alpha-hydroxylase,17,20-lyase (p450c17) is a key steroidogenic enzyme in the production of androgens and, therefore, is also indispensable for the production of oestrogens (that are produced from the aromatisation of androgens). in this study, p450c17 cdna was cloned from the ovary of the fathead minnow (fhm) and its gene expression was examined in the gonads and brains of male and female fhm at different stages of gonadal development with a view to developing an understanding of its invo ... | 2003 | 12606268 |
| systemic and cutaneous mucus antibody responses of channel catfish immunized against the protozoan parasite ichthyophthirius multifiliis. | fish acquire protective immunity against the ciliated protozoan parasite ichthyophthirius multifiliis following sublethal infection or inoculation with i. multifiliis immobilization antigens (i-antigens). in both cases, parasite-immobilizing antibodies have been identified in sera and mucosal secretions. to investigate the kinetics of this immune response, antibody levels were determined by enzyme-linked immunosorbent assay (elisa) in the sera and cutaneous mucus of channel catfish (ictalurus pu ... | 2003 | 12965920 |
| the edwardsiella ictaluri o polysaccharide biosynthesis gene cluster and the role of o polysaccharide in resistance to normal catfish serum and catfish neutrophils. | edwardsiella ictaluri, the causative agent of enteric septicaemia of catfish (esc), expresses long o polysaccharide (ops) chains on its surface. the authors previously reported the construction of an isogenic ed. ictaluri ops mutant strain and demonstrated that this strain is avirulent in channel catfish. this paper reports the cloning of the ed. ictaluri ops biosynthesis gene cluster and identification of the mutated gene in the ops-negative strain. the sequenced region contains eight complete ... | 2003 | 12777482 |
| rapid detection of columnaris disease in channel catfish (ictalurus punctatus) with a new species-specific 16-s rrna gene-based pcr primer for flavobacterium columnare. | a 16-s rrna gene from the chromosomal dna of the fish-pathogenic bacterium flavobacterium columnare (formerly flexibacter columnaris), strain ars-i, was cloned, sequenced and used to design a polymerase chain reaction (pcr) primer set. the primer set amplified a specific 1193-bp dna fragment from f. columnare strains but not from related bacteria, f. psychrophilum, f. aquatile, f. branchiophilum, or other bacterial pathogens of fish, flexibacter maritimus, cytophaga johnsonae, edwardsiella ictal ... | 2003 | 12459241 |
| disparate regulation of insulin-like growth factor-binding proteins in a primitive, ictalurid, teleost (ictalurus punctatus). | vertebrate growth is principally controlled by growth hormone (gh) and, its intermediary, insulin-like growth factor-i (igf-i). the actions of igf-i are modulated by high-affinity binding proteins called insulin-like growth factor binding-proteins (igfbps). channel catfish exhibit atypical responses (increased percentage body fat and reduced percentage protein) to gh treatment, despite gh-dependent igf-i production. among possible explanations for this atypical response to gh treatment is an unu ... | 2003 | 14511982 |
| feed deprivation of channel catfish, ictalurus punctatus (rafinesque), influences organosomatic indices, chemical composition and susceptibility to flavobacterium columnare. | withholding feed has been suggested as a strategy to manage infectious disease of channel catfish, ictalurus punctatus (rafinesque). in a previous study, we demonstrated that deprivation of feed for as little as 7 days reduced innate resistance of catfish to flavobacterium columnare. this study was conducted to evaluate the effect of feeding regimens [no feeding (nf), fed once every other day to satiation (feod) and fed once daily to satiation (fd)] on organosomatic indices, physiological change ... | 2003 | 14575373 |
| safety of aquaflor (florfenicol, 50% type a medicated article), administered in feed to channel catfish, ictalurus punctatus. | aquaflor, a feed premix containing the broad spectrum antibacterial agent florfenicol (50% w/w), is being developed for use to control enteric septicemia (esc) in channel catfish ictalurus punctatus caused by the gram-negative enterobacterium edwardsiella ictaluri. the recommended dose of aquaflor to control esc is 10 mg/kg body weight (bw)/day for 10 days. the study objective was to determine the safety of aquaflor administered in feed to channel catfish at doses of 0 (control), 10, 30, and 50 ... | 2003 | 14585738 |
| inductively coupled plasma optical emission spectrometric determination of minerals in catfish frame. | the concentration of 14 major and trace elements in catfish (ictalurus punctatus) frames was determined and analyzed for differences. three frames were obtained each week for five consecutive weeks in the spring and the fall of 2000. the frames were freed of remaining flesh, dried, treated by microwave dissolution technique and the minerals determined by inductively coupled plasma optical emission spectrometry (icp-oes). the mean calcium concentration (mg/kg, dry basis) in the spring (140400) wa ... | 2003 | 14609085 |
| cloning, sequence and variability analysis of expressed immunoglobulin light chain genes from yellowtail seriola quinqueradiata. | the cdna clones encoding immunoglobulin (ig) light (l) chain variable (v) region associated with constant (c) region were isolated from yellowtail (seriola quinqueradiata) kidney by expressed sequence tag analysis (accession numbers: ab062619-ab062668, ab064322). the sequences of both vl and cl region contain well-conserved cysteine residues important for intra- and inter-domain interaction in mammals. comparisons of the amino acid sequence of the cl domain with those of other species showed a h ... | 2003 | 12547626 |
| putative snp discovery in interspecific hybrids of catfish by comparative est analysis. | in this study, we identified putative snp markers within genes by comparative analysis of expressed sequence tags (ests). comparison of 849 ests from blue catfish (ictalurus furcatus) with >11,000 ests from channel catfish (i. punctatus) deposited in genbank resulted in the identification of 1020 putative snps within 161 genes, of which 145 were nuclear genes of known function. the observed frequency of snps within ests of the two closely related catfish species was 1.32 snp per 100 bp. the majo ... | 2003 | 14687075 |
| viral susceptibility of newly established cell lines from the hawaiian monk seal monachus schauinslandi. | ten of 11 cell lines, recently established from the snout (ms-sn), periorbital soft tissue (ms-ey), liver (ms-lv), kidney (ms-kd), lung (ms-lg), spleen (ms-sp), heart (ms-ht), thyroid (ms-ty), brain (ms-br) and urinary bladder (ms-ub) of a juvenile hawaiian monk seal monachus schauinslandi, were evaluated in vitro for their susceptibility to 5 mammalian viruses: herpes simplex virus type 1 (hsv-1), vesicular stomatitis virus (vsv), reovirus type 3 (reo-3), poliovirus type 1 (polio-1) and vaccini ... | 2003 | 14960030 |
| comparative challenge model of flavobacterium columnare using abraded and unabraded channel catfish, ictalurus punctatus (rafinesque). | the early entry of the fish pathogen flavobacterium columnare and enhancement by abrasion was studied in channel catfish, ictalurus punctatus (rafinesque), using the polymerase chain reaction and a species-specific primer set for a bacterial 16s rrna gene product. evaluations were conducted following an abrasion bath immersion challenge with f. columnare. abrasion, a practice which has historically been used prior to bacterial challenge, had significant effects on the early entry of the pathogen ... | 2003 | 14513970 |
| channel catfish virus gene expression in experimentally infected channel catfish, ictalurus punctatus (rafinesque). | channel catfish virus (ccv) produces an acute haemorrhagic disease in fingerling channel catfish and establishes latent infection in fish that survive the primary infection. this study investigated ccv gene expression in tissues of experimentally infected fish. reverse transcriptase polymerase chain reaction assays were developed for detection of transcripts expressed by each of the ccv direct repeat region genes in ccv-infected channel catfish ovary cells and in tissues of infected fish. immedi ... | 2003 | 14513973 |
| in vitro evaluation of the susceptibility of edwardsiella ictaluri, etiological agent of enteric septicemia in channel catfish, ictalurus punctatus (rafinesque), to florfenicol. | in vitro studies were conducted to assess the sensitivity of edwardsiella ictaluri, the etiological agent of enteric septicemia of catfish (esc), to the antibacterial drug florfenicol (ffc). twelve different e. ictaluri isolates from cases submitted between 1994 and 1997 to the thad cochran national warmwater aquaculture center fish diagnostic laboratory (stoneville, ms) were used for testing. these isolates originated from channel catfish (ictalurus punctatus) infected with e. ictaluri through ... | 2003 | 14667023 |
| cadmium effects on ichthyophthirius: evidence for metal-sequestration in fish tissues following administration of recombinant vaccines. | we are developing tetrahymena thermophila as a delivery system for recombinant vaccines against parasitic protozoa, including the common fish parasite, ichthyophthirius multifiliis. t. thermophila cell lines expressing i. multifiliis genes under the control of a cadmium-inducible metallothionein gene promoter conferred strong protection against a lethal parasite challenge when administered parenterally to naive fish. nevertheless, given that heavy metals can be toxic to parasites, a question aro ... | 2003 | 14667175 |
| distribution of mercury in the tissues of five species of freshwater fish from lake mead, usa. | total mercury (hg) concentrations were determined in seven tissues (skeletal muscle, liver, blood, gonad, brain, gill, and heart) of 59 striped bass and four tissues (muscle, liver, blood, and gonad) of 69 largemouth bass, 76 channel catfish, 12 bluegill, and 22 blue tilapia collected from lake mead, usa. mercury levels generally increased according to trophic level and fish length. for striped bass, mean hg levels (ng g(-1), wet mass) were highest in the liver (531), followed by muscle (309), h ... | 2003 | 14587853 |
| cloning and characterisation of natural resistance associated macrophage protein (nramp) cdna from red sea bream (pagrus major). | nramp (natural resistance associated macrophage protein) controls aspects of innate resistance to intracellular parasites. its function is to enhance the ability of macrophages to kill pathogens. however, little is known about the structure and function of nramp in lower vertebrates such as teleosts. we have recently isolated a cdna encoding nramp from spleen of red sea bream (pagrus major). the full-length cdna of the nramp is 4709 bp in length, including 197 bp 5'-terminal untranslated region ... | 2004 | 15312657 |
| interaction of xenobiotics with estrogen receptors alpha and beta and a putative plasma sex hormone-binding globulin from channel catfish (ictalurus punctatus). | estrogens are important regulators of physiological functions. although environmental contaminants (xenoestrogens) which interfere with estrogen signaling are of increasing concern, there is only limited information about their ability to interact with estrogen-binding proteins (shbg) or receptors (er). recombinant eralpha and beta were obtained after transient transfection of cos-7 cells with channel catfish er cdna. plasma from adult female channel catfish was the source of shbg. tritiated est ... | 2004 | 15081833 |
| activation of tlr3 and tlr5 in channel catfish exposed to virulent edwardsiella ictaluri. | two toll-like receptors (tlr3 and tlr5) were identified from a catfish cdna fry library based on sequence similarity to other vertebrate tlr genes. expression (using real-time pcr) of tlr3 and tlr5 was measured for two strains of channel catfish in previously non-exposed fish 2, 5, 8, and 21 days after experimental edwardsiella ictaluri challenge to determine if tlrs are associated with host response to e. ictaluri infection. expression of tlr5 was higher than tlr3 (p<0.0001). tlr3 expression in ... | 2004 | 15854683 |
| evidence for the existence of granzyme-like serine proteases in teleost cytotoxic cells. | granzymes are granule-associated serine proteases, which are important effector molecules in nk cell and ctl functions. the granzyme family poses a perplexing problem in phylogenetics due to the lack of nonmammalian sequence information. we now report the identification of a cdna that codes for a granzyme homologue, channel catfish granzyme-1 (cfgr-1), from nonspecific cytotoxic cells (ncc) of a teleost. ncc are the first identified and extensively studied cytotoxic cell population in teleosts. ... | 2004 | 15114423 |
| defeating diplostomoid dangers in usa catfish aquaculture. | diplostomoid digenean metacercariae have caused widescale mortalities of channel catfish, ictalurus punctatus (rafinesque), at aquaculture farms in louisiana, mississippi and arkansas, usa. originally, based on a tentative diagnosis, the industry considered the primary harmful agent to be an introduced species from europe, bolbophorus confusus (krause, 1914), frequently reported from the american white pelican, pelecanus erythrorhynchos gmelin. our group has now shown, using its 1-2 plus three m ... | 2004 | 15357393 |
| evolution of transcriptional control of the igh locus: characterization, expression, and function of tf12/heb homologs of the catfish. | the transcriptional enhancer (emu3') of the igh locus of the channel catfish, ictalurus punctatus, differs from enhancers of the mammalian igh locus in terms of its position, structure, and function. transcription factors binding to multiple octamer motifs and a single mue5 motif (an e-box site, consensus canntg) interact for its function. e-box binding transcription factors of the class i basic helix-loop-helix family were cloned from a catfish b cell cdna library in this study, and homologs of ... | 2004 | 15494495 |
| estima, a tool for est management in a multi-project environment. | single-pass, partial sequencing of complementary dna (cdna) libraries generates thousands of chromatograms that are processed into high quality expressed sequence tags (ests), and then assembled into contigs representative of putative genes. usually, to be of value, ests and contigs must be associated with meaningful annotations, and made available to end-users. | 2004 | 15527510 |
| parasites of native and nonnative fishes of the little colorado river, grand canyon, arizona. | a 2-yr, seasonal, parasitological study of 1,435 fish, belonging to 4 species of native fishes and 7 species of nonnative fishes from the lower little colorado river (lcr) and tributary creeks, grand canyon, arizona, yielded 17 species of parasites. these comprised 1 myxozoan (henneguya exilis), 2 copepods (ergasilus arthrosis and lernaea cyprinacea), 1 acarine (oribatida gen. sp.), 1 piscicolid leech (myzobdella lugubris), 4 monogeneans (gyrodactylus hoffmani, gyrodactylus sp., dactylogyrus ext ... | 2004 | 15562604 |
| cloning and characterization of myogenic regulatory genes in three ictalurid species. | we report sequence, tissue expression and map-position data for myogenin, myod1, myostatin and follistatin in three ictalurid catfish species: channel catfish (ictalurus punctatus), blue catfish (i. furcatus) and white catfish (ameiurus catus). these genes are involved in muscle growth and development in mammals and may play similar roles in catfish. amino acid sequences were highly conserved among the three ictalurid species (>95% identity), moderately conserved among catfish and zebrafish (app ... | 2004 | 15566463 |
| susceptibility of channel catfish fry to channel catfish virus (ccv) challenge increases with age. | susceptibility of channel catfish to channel catfish virus disease (ccvd) has been generally considered to be inversely related to age. however, in experimental immersion challenges, we found that channel catfish fry, 3 to 8 d post hatch (dph), are most resistant to ccv and susceptibility increases with age. initial studies involved 2 spawns that had high ccv carrier percentage. to determine if the resistance seen in the fry was related to the ccv carrier status of the parents, we selected 4 spa ... | 2004 | 15648828 |
| genetic fingerprinting of flavobacterium columnare isolates from cultured fish. | to evaluate the intraspecific diversity of the fish pathogen flavobacterium columnare. | 2004 | 15239710 |
| multiple cc chemokines in channel catfish and blue catfish as revealed by analysis of expressed sequence tags. | chemokines represent a superfamily of chemotactic cytokines involved in recruitment, activation and adhesion of a variety of leukocyte types to inflammatory foci, as well as in the organization and maintenance of lymphoid organ architecture and in normal developmental processes. nearly all chemokines have been identified in human and mouse, but only a handful of fish chemokines have been identified. here we describe 14 distinct chemokines from channel catfish and blue catfish identified by analy ... | 2004 | 15309344 |
| cloning and characterisation of a channel catfish (ictalurus punctatus) mx gene. | a 2.5 kb full-length cdna clone of a channel catfish (ictalurus punctatus) mx gene was obtained using race (rapid amplification of cdna ends) polymerase chain reaction (pcr) from rna extracted from the liver of poly i:c stimulated channel catfish. the gene consists of an open reading frame of 1905 nucleotides encoding a 635 amino acid protein. the predicted protein is 72.5 kda and contains the dynamin family signature, a tripartite gtp binding motif and a leucine zipper, characteristic of all kn ... | 2004 | 15123306 |
| immune response induced by n-lauroylsarcosine extracted outer-membrane proteins of an isolate of edwardsiella ictaluri in channel catfish. | a virulent isolate of edwardsiella ictaluri (al-93-75), the causative agent of enteric septicaemia of catfish (esc), was used to derive a lipopolysaccharide-reduced n-lauroylsarcosine outer-membrane protein (omp) fraction vaccine. the omp fraction was analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and compared to whole-cell lysate, purified lipopolysaccharide (lps) and a crude cell-wall fraction. the omp fraction contained less than 2% (w/v) lps. sds-page sho ... | 2004 | 15123308 |
| mycobacteria, but not mercury, induces metallothionein (mt) protein in striped bass, morone saxitilis, phagocytes, while both stimuli induce mt in channel catfish, ictalurus punctatus, phagocytes. | recent advances in molecular immunology indicate that the expression of inducible pro-inflammatory proteins is increased in vertebrates in response to both infectious disease agents and various xenobiotics. for example, inos, cox-2, and cyp1a are induced by both inflammation and ahr ligands. moreover, the expression of these proteins in response to stimuli varies among individuals within populations. little is known of the differences among fish in the inducibility of proinflammatory proteins in ... | 2004 | 15178104 |
| inactivation of viruses infecting ectothermic animals by amphibian and piscine antimicrobial peptides. | the ability of five purified amphibian antimicrobial peptides (dermaseptin-1, temporin a, magainin i, and ii, pgla), crude peptide fractions isolated from the skin of rana pipiens and r. catesbeiana, and four antimicrobial peptides (amps) from hybrid striped bass (piscidin-1n, -1h, -2, and -3) were examined for their ability to reduce the infectivity of channel catfish virus (ccv) and frog virus 3 (fv3). all compounds, with the exception of magainin i, markedly reduced the infectivity of ccv. in ... | 2004 | 15193922 |
| two year study on the infectivity of ichthyophthirius multifiliis in channel catfish ictalurus punctatus. | ichthyophthirius multifiliis fouquet (ich) is a fish parasite that causes serious economic loss for aquaculture. a major difficulty in the maintenance of single isolates of ich for research purposes is the loss of infectivity. after an unknown number of passages or infection cycles the ich isolate loses its infectivity. this study determined the infectivity of an ich isolate during 105 infection cycles in channel catfish ictalurus punctatus over a 2 yr period. the mean percentage of fish infecte ... | 2004 | 15212279 |
| pentachlorophenol-induced cytotoxic, mitogenic, and endocrine-disrupting activities in channel catfish, ictalurus punctatus. | pentachlorophenol (pcp) is an organochlorine compound that has been widely used as a biocide in several industrial, agricultural, and domestic applications. although it has been shown to induce systemic toxicity and carcinogenesis in several experimental studies, the literature is scarce regarding its toxic mechanisms of action at the cellular and molecular levels. recent investigations in our laboratory have shown that pcp induces cytotoxicity and transcriptionally activates stress genes in hum ... | 2004 | 16696183 |
| acute columnaris infection in channel catfish, ictalurus punctatus (rafinesque): efficacy of practical treatments for warmwater aquaculture ponds. | columnaris disease was induced in channel catfish, ictalurus punctatus (rafinesque), by bath exposure to four highly virulent isolates of flavobacterium columnare. in untreated controls, mortality began 20 h after exposure and reached 100% by 48 h. mortality in channel catfish given antibiotic treatments with oxytetracycline or a combination of sulphadimethoxine and ormetoprim in feed prior to bacterial challenge was zero with all four strains of f. columnare. diquat (zeneca agricultural product ... | 2004 | 14986936 |
| morphological and genetic characteristics of flavobacterium columnare isolates: correlations with virulence in fish. | variability in pathogenicity of flavobacterium columnare makes disease treatment difficult because there is currently no way to easily recognize those strains that warrant aggressive treatments. in order to identify suitable virulence markers, 17 isolates of f. columnare were cultured from six different fish species. the dna from all isolates was analysed using random amplified polymorphic dna (rapd). bootstrap analysis of the rapd data produced a tree with three major groups supported by bootst ... | 2004 | 14986937 |
| immune responses and host protection of channel catfish, ictalurus punctatus (rafinesque), against ichthyophthirius multifiliis after immunization with live theronts and sonicated trophonts. | the humoral immune responses and host protection of channel catfish, ictalurus punctatus (rafinesque), against ichthyophthirius multifiliis (ich) were determined after immunization with live theronts and sonicated trophonts. immunizations with live theronts or sonicated trophonts were carried out by both bath immersion and intraperitoneal (i.p.) injection. cutaneous and serum immunoglobulin (ig) levels and anti-ich antibodies were measured 12 and 21 days post-immunization. the level of ich infec ... | 2004 | 15009239 |
| cloning and expression of the aid gene in the channel catfish. | a full-length activation-induced cytidine deaminase (aid or aicda) cdna has been obtained from the channel catfish (ictalurus punctatus). a single open reading frame predicts a 209 amino acid protein that has 57% identity and 73% similarity with the aid proteins of mouse and human. all residues that have previously been found to be critical for deamination, as well as for somatic hypermutation, are conserved in the catfish aid. these residues are also conserved in aid proteins predicted, from ge ... | 2004 | 15043936 |
| sequence analysis and expression of a cxc chemokine in resistant and susceptible catfish after infection of edwardsiella ictaluri. | chemokines represent a superfamily of chemotactic cytokines involved in recruitment, activation and adhesion of a variety of leukocyte types to inflammatory foci. we cloned and sequenced the cdna of a cxc chemokine that is most similar to cxcl10 from channel catfish and blue catfish. sequence analysis of pcr amplicons from a single f1 hybrid catfish indicated that channel catfish and blue catfish may have a multigene family for the cxc chemokine. the catfish cxc chemokine was expressed in a wide ... | 2004 | 15043945 |
| purification of antimicrobial factor from granules of channel catfish peripheral blood leucocytes. | the channel catfish (ictalurus punctatus) is extensively used in aquaculture in the southeast us and is susceptible to many bacterial infections acquired from its pond environment. research is needed to better understand the defensive response and innate immunity of channel catfish against fish pathogens like edwardsiella ictaluri and aeromonas hydrophila. the main objectives were purification and characterization of an innate antimicrobial factor isolated from catfish leucocytes that has both b ... | 2004 | 14672693 |
| identification of a cdna encoding channel catfish interferon. | despite considerable advances in our understanding of teleost immunity, relatively few cytokine genes, including those for interferon (ifn), have been identified at the molecular level. in contrast, numerous studies have shown that following virus infection or exposure to double-stranded rna, fish or fish cells produce a soluble factor that is functionally similar to mammalian ifn. a putative catfish (cf) ifn cdna was identified by blastx screening of a catfish est library generated from a mixed ... | 2004 | 12969796 |
| molecular cloning of proopiomelanocortin cdna and multi-tissue mrna expression in channel catfish. | channel catfish (ictalurus punctatus) proopiomelanocortin (pomc) cdna was cloned to investigate its structure, evolution, and expression in different tissues. pomc is an important gene in the hypothalamus-pituitary-adrenal axis, the main mediator of the stress response. pomc gene was isolated from a pituitary cdna library and nucleotide sequence was determined. pomc cdna is composed of 1164 nucleotides with a 639 nucleotide open reading frame encoding a protein of 212 amino acids. catfish pomc p ... | 2004 | 15201069 |
| genes of the constant regions of functional immunoglobulin heavy chain of japanese flounder, paralichthys olivaceus. | igm and igd genes of the japanese flounder were cloned and characterized from a genomic bacterial artificial chromosome (bac) library. the micro gene contained four constant region exons (cmi1-cmi4), and two transmembrane exons (mitm1 and mitm2), which conforms to the organizational pattern of all other vertebrate micro-chain genes examined so far. in the same bac clone, the delta gene, which is homologous to the igd gene in mammals and teleost fish, was found immediately (0.9 kb) downstream of ... | 2004 | 15243720 |
| evolution of transcriptional enhancers in the immunoglobulin heavy-chain gene: functional characteristics of the zebrafish emu3' enhancer. | past studies of the channel catfish immunoglobulin heavy-chain (igh) locus indicates that it lacks an emu enhancer in the j(h)-cmu1 intron but does have an enhancer, termed emu3', in the mu-delta intergenic region. the positioning of the catfish enhancer downstream of the mu-chain exons is predicted to be unfavorable for antibody-affinity maturation in catfish, and would also have been an impediment to the evolution of class switch recombination, had it existed in early tetrapods. to determine i ... | 2005 | 15756545 |
| biochemical effects of lead, zinc, and cadmium from mining on fish in the tri-states district of northeastern oklahoma, usa. | we assessed the exposure of fish from the spring and neosho rivers in northeast oklahoma, usa, to lead, zinc, and cadmium from historical mining in the tri-states mining district (tsmd). fish (n = 74) representing six species were collected in october 2001 from six sites on the spring and neosho rivers influenced to differing degrees by mining. additional samples were obtained from the big river, a heavily contaminated stream in eastern missouri, usa, and from reference sites. blood from each fi ... | 2005 | 16117126 |