Publications

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mosquito (diptera: culicidae) surveillance for arboviruses in an area endemic for west nile (lineage rabensburg) and tahyna viruses in central europe.six viral isolates were obtained from 23,243 female mosquitoes (examined in 513 pools) belonging to 16 species and collected along the lower reaches of the dyje river in south moravia (czech republic, central europe) during 2006-2008: five isolates of orthobunyavirus tahyna (tahv, california group, family bunyaviridae: three isolations from aedes vexans (meigen), one from ae. sticticus (meigen), one from culex modestus ficalbi); and one isolation of flavivirus west nile (wnv, japanese encephalit ...201020496595
genetic diversity of aedes vexans (diptera, culicidae) from new orleans: pre- and post-katrina.the floodwater mosquito aedes vexans, a potential vector of west nile virus, has a worldwide distribution that includes the continental united states and southern canada. in order to determine the effect that hurricane katrina had on the temporal genetic variation of ae. vexans from new orleans, we compared genetic diversity of a portion of the mtdna nd5 gene of mosquito specimens collected during 2005 (n = 99) with specimens from 2006 (n = 103), after the hurricane. average haplotype diversity ...201020526734
culicinae mosquitoes in sanandaj county, kurdistan province, western iran.this study aims at studying mosquito-borne diseases as the major public health threat in iran. sanandaj outskirts are considered suitable habitats for mosquito larvae. in view of scanty reports on mosquito-borne disease implementation in this area, a study was undertaken to determine the mosquito fauna and frequency of mosquito larvae at sanandaj city.201020539048
mosquito (diptera: culicidae) fauna in an area endemic for west nile virus.mosquito collections with cdc light traps using dry ice and pigeon-baited traps were carried out in south moravia (czech republic) from april to october in 2007 and 2008 at two study sites. in 2007, 11 two-day captures were carried out in two-week intervals, and 1,490 female mosquitoes of nine species were caught. in 2008, 15 two-day trappings of mosquitoes were carried out: 6,778 females of 22 species of mosquitoes were trapped. the results showed marked differences in abundance and species com ...201020618662
seasonality and time of host-seeking activity of culex tarsalis and floodwater aedes in northern colorado, 2006-2007.effective and economical control of adult vector and pest mosquitoes requires knowledge of their seasonal abundance and host-seeking activity patterns. we conducted research in 2006-2007 to study these variables for culex tarsalis, aedes vexans, ae. melanimon, and ae. dorsalis in larimer county, co. mosquitoes were collected with traps that segregated catches in 7 consecutive 2-h intervals initiating at 1730 h at 4 sites. seasonal abundance varied for all species by site and year. time of host-s ...201020649124
[current status and eco-epidemiology of mosquito-borne arboviruses (diptera: culicidae) in spain].in this manuscript we analize the possible emergence and/or re-emergence in spain of some of the mosquito-borne arboviruses (diptera: culicidae) with highest incidence in recent years. the faunistic, bioecological and distributional data of the culicids in our country allow to differentiate between species with ability to maintain the enzootic cycles of arboviruses from others that can act as bridge vectors to the human population. the results show the existence of several common and anthropophi ...201020661525
rift valley fever virus epidemic in kenya, 2006/2007: the entomologic investigations.in december 2006, rift valley fever (rvf) was diagnosed in humans in garissa hospital, kenya and an outbreak reported affecting 11 districts. entomologic surveillance was performed in four districts to determine the epidemic/epizootic vectors of rvf virus (rvfv). approximately 297,000 mosquitoes were collected, 164,626 identified to species, 72,058 sorted into 3,003 pools and tested for rvfv by reverse transcription-polymerase chain reaction. seventy-seven pools representing 10 species tested po ...201020682903
effects of larval growth condition and water availability on desiccation resistance and its physiological basis in adult anopheles gambiae sensu stricto.natural populations of the malaria mosquito anopheles gambiae s.s. are exposed to large seasonal and daily fluctuations in relative humidity and temperature, which makes coping with drought a crucial aspect of their ecology.201020691104
detection of dirofilaria immitis (nematoda: filarioidea) by polymerase chain reaction in aedes albopictus, anopheles punctipennis, and anopheles crucians (diptera: culicidae) from georgia, usa.potential mosquito vectors of dirofilaria immitis (leidy) (nematoda: filarioidea), the causative agent of dog heartworm in the southeastern region of the united states, were collected with cdc light traps and gravid traps in seven counties in the state of georgia, usa. the presence of d. immitis in these mosquitoes was detected by polymerase chain reaction using species-specific primers for the d. immitis surface or cuticular antigen. overall, 1,574 mosquitoes of 13 species in seven genera were ...201020695279
detection of francisella tularensis in alaskan mosquitoes (diptera: culicidae) and assessment of a laboratory model for transmission.tularemia is a zoonotic disease caused by the category a bioterrorism agent francisella tularensis. in scandinavia, tularemia transmission by mosquitoes has been widely cited in the literature. we tested >2,500 mosquitoes captured in alaska and found francisella dna in 30% of pooled samples. to examine the potential for transmission of francisella by mosquitoes, we developed a mosquito model of francisella infection. larvae of anopheles gambiae giles and aedes aegypti (l.) readily ingest f. tula ...201020695280
heritability and adaptive phenotypic plasticity of adult body size in the mosquito aedes aegypti with implications for dengue vector competence.adaptive phenotypic plasticity is particularly important to organisms with developmental cycles that undergo ontogenetic niche shifts that differentially subject individual life stages to heterogeneous and often stressful environmental conditions. the yellow fever and dengue fever vector mosquito, aedes aegypti, typically breeds in small water-filled containers that expose the developing aquatic larvae to competition for resources with conspecifics and high probabilities for habitat drying. here ...201021070891
planning for rift valley fever virus: use of geographical information systems to estimate the human health threat of white-tailed deer (odocoileus virginianus)-related transmission.rift valley fever (rvf) virus is a mosquito-borne phlebovirus of the bunyaviridae family that causes frequent outbreaks of severe animal and human disease in sub-saharan africa, egypt and the arabian peninsula. based on its many known competent vectors, its potential for transmission via aerosolization, and its progressive spread from east africa to neighbouring regions, rvf is considered a high-priority, emerging health threat for humans, livestock and wildlife in all parts of the world. introd ...201021080319
eastern equine encephalitis virus in mosquitoes and their role as bridge vectors.eastern equine encephalitis virus (eeev) is maintained in an enzootic cycle involving culiseta melanura mosquitoes and avian hosts. other mosquito species that feed opportunistically on mammals have been incriminated as bridge vectors to humans and horses. to evaluate the capacity of these mosquitoes to acquire, replicate, and potentially transmit eeev, we estimated the infection prevalence and virus titers in mosquitoes collected in connecticut, usa, by cell culture, plaque titration, and quant ...201021122215
aedes vexans and culex pipiens as the potential vectors of dirofilaria immitis in central turkey.this study was carried out to investigate the potential vectors and relative mosquito infection rates of dirofilaria immitis throughout two mosquito seasons (2008-2009) in kayseri province where is located in central anatolian part of turkey. for this aim, totally 1198 genomic dna pools, extracted and grouped according to the species and collection site (1-17 specimens/pool) from 6153 mosquito specimens, were examined by pcr using species-specific primers for d. immitis. the captured mosquitoes ...201021232866
aedes aegypti: an emerging model for vector mosquito development.blood-feeding mosquitoes, including the dengue and yellow fever vector aedes aegypti, transmit many of the world's deadliest diseases. such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. recent mosquito genome projects have stimulated interest in the potential for arthropod-borne disease control by genetic manipulation of vector insects. targets of particular interest include genes that regulate development. however, although ...201020889691
species abundance and seasonal activity of mosquitoes on cattle facilities in southern alberta, canada.seventeen mosquito species were collected at eight cattle facilities in southern alberta, canada, during 2002-2004. five species, culiseta inornata (williston), aedes dorsalis (meigen), culex tarsalis (coquillett), aedes vexans (meigen), and aedes campestris dyar & knab accounted for 93.6% of the mosquitoes collected. culiseta inornata, ae. dorsalis, and ae. campestris were trapped earliest in the year. cs. inornata was active latest in the year and had the longest period of activity. ae. dorsal ...201020180306
developing gis-based eastern equine encephalitis vector-host models in tuskegee, alabama.a site near tuskegee, alabama was examined for vector-host activities of eastern equine encephalomyelitis virus (eeev). land cover maps of the study site were created in arcinfo 9.2 from quickbird data encompassing visible and near-infrared (nir) band information (0.45 to 0.72 microm) acquired july 15, 2008. georeferenced mosquito and bird sampling sites, and their associated land cover attributes from the study site, were overlaid onto the satellite data. sas 9.1.4 was used to explore univariat ...201020181267
lack of evidence for the presence of mosquito-borne arboviruses in the upper rhine valley, germany, in 1999 to 2000. 201020592140
ecological approaches to informing public health policy and risk assessments on emerging vector-borne zoonoses.pathogens associated with vector-borne zoonoses occur in enzootic cycles within nature. they are driven by a combination of vertebrate host and invertebrate vector population dynamics, which in turn respond to changes in environmental stimuli. human involvement in these cycles, and hence the occurrence of human disease, is often to act as incidental host. from a public health perspective our ability to better predict human outbreaks of these diseases and prepare intervention and mitigation strat ...201022460391
larval habitat characteristics of the genus anopheles (diptera: culicidae) and a checklist of mosquitoes in guilan province, northern iran.ecological data are important in the vector control management of mosquitoes. there is scattered published information about the larval habitat characteristics and ecology of the genus anopheles (diptera: culicidae) in iran and most of available data is in relation to malaria vectors in southern iran.201122808409
tabanidae and other diptera on camel's hump vermont: ecological observations.a canopy trap and aerial nets led to finding 8 species of tabanidae. there was an abundance of calyptrate muscoid flies. camel's hump is in the green mountains of western new england, usa. discovering diptera on camel's hump involved sixteen visits over 40 years. upwards of 23 other diptera species are listed. habitats on the east side and above 762 m (2500 ft) elevation on camel's hump differ from the west slope but the boreal forest on both sides is influenced by cloud and fog precipitation on ...201122371675
newly recognized mosquito-associated viruses in mainland china, in the last two decades.there are four principal arboviruses in mainland china. two kinds of them are mosquito-borne viruses, namely japanese encephalitis virus and dengue virus, which lead to japanese encephalitis, and dengue fever/dengue hemorrhagic fever respectively; the other two are tick-borne viruses, namely tick-borne encephalitis virus and crimean-congo hemorrhagic fever virus (also known as xinjiang hemorrhagic fever virus), which contribute to tick-borne encephalitis and xinjiang hemorrhagic fever respective ...201121314994
temporal analysis of feeding patterns of culex erraticus in central alabama.host blood meals in seven mosquito species previously shown to be infected with eastern equine encephalitis virus at a site in the tuskegee national forest in southcentral alabama were investigated. of 1374 blood meals derived from 88 different host species collected over 6 years from these seven mosquito species, 1099 were derived from culex erraticus. analysis of the temporal pattern of cx. erraticus meals using a runs test revealed that the patterns of feeding upon avian and mammalian hosts f ...201121395423
host reproductive phenology drives seasonal patterns of host use in mosquitoes.seasonal shifts in host use by mosquitoes from birds to mammals drive the timing and intensity of annual epidemics of mosquito-borne viruses, such as west nile virus, in north america. the biological mechanism underlying these shifts has been a matter of debate, with hypotheses falling into two camps: (1) the shift is driven by changes in host abundance, or (2) the shift is driven by seasonal changes in the foraging behavior of mosquitoes. here we explored the idea that seasonal changes in host ...201121408172
humoral response to the anopheles gambiae salivary protein gsg6: a serological indicator of exposure to afrotropical malaria vectors.salivary proteins injected by blood feeding arthropods into their hosts evoke a saliva-specific humoral response which can be useful to evaluate exposure to bites of disease vectors. however, saliva of hematophagous arthropods is a complex cocktail of bioactive factors and its use in immunoassays can be misleading because of potential cross-reactivity to other antigens. toward the development of a serological marker of exposure to afrotropical malaria vectors we expressed the anopheles gambiae g ...201121437289
distribution of bacillus thuringiensis subsp. israelensis in soil of a swiss wetland reserve after 22 years of mosquito control.recurrent treatments with bacillus thuringiensis subsp. israelensis are required to control the floodwater mosquito aedes vexans that breeds in large numbers in the wetlands of the bolle di magadino reserve in canton ticino, switzerland. interventions have been carried out since 1988. in the present study, the spatial distribution of resting b. thuringiensis subsp. israelensis spores in the soil was measured. the b. thuringiensis subsp. israelensis concentration was determined in soil samples co ...201121498758
a multi-year study of mosquito feeding patterns on avian hosts in a southeastern focus of eastern equine encephalitis virus.abstract. eastern equine encephalitis virus (eeev) is a mosquito-borne pathogen that cycles in birds but also causes severe disease in humans and horses. we examined patterns of avian host use by vectors of eeev in alabama from 2001 to 2009 using blood-meal analysis of field-collected mosquitoes and avian abundance surveys. the northern cardinal (cardinalis cardinalis) was the only preferred host (fed on significantly more than expected based on abundance) of culiseta melanura, the enzootic vect ...201121540380
altitudinal variation in the morphometric characteristics of aedes vexans meigen from northeastern turkey.body size is one of the most significant features of organisms and is correlated with a large number of ecological and physiological variables. similar to size, biological shape is one of the most conspicuous aspects of an organism's phenotype and provides a link between the genotype and the environment. body size may change with altitude and also habitat differences associated with altitude may affect the biological shape and some morphological characteristics. four populations of aedes vexans ...201121635639
feeding behaviour of potential vectors of west nile virus in senegal.west nile virus (wnv) is a widespread pathogen maintained in an enzootic cycle between mosquitoes and birds with occasional spill-over into dead-end hosts such as horses and humans. migratory birds are believed to play an important role in its dissemination from and to the palaearctic area, as well as its local dispersion between wintering sites. the djoudj park, located in senegal, is a major wintering site for birds migrating from europe during the study period (sept. 2008- jan. 2009). in this ...201121651763
weather and land cover influences on mosquito populations in sioux falls, south dakota.this study compared the spatial and temporal patterns of culex tarsalis coquillett and aedes vexans meigen populations and examined their relationships with land cover types and climatic variability in sioux falls, sd. between 24 and 30 cdc co2-baited light traps were set annually in sioux falls from may to september 2005-2008. land cover data were acquired from the 2001 national land cover dataset and the percentages of selected land cover types were calculated within a 600-m buffer zone around ...201121661329
the mosquito aedes (aedimorphus) vexans arabiensis as a probable vector bridging the west nile virus between birds and horses in barkedji (ferlo, senegal).active catches of adult females of aedes vexans arabiensis patton, (diptera: culicidae) patton by nets or aspirator, were conducted in 2003 and 2004 in the vegetation at the edge of temporary ponds in barkedji, senegalese ferlo area. two hundred and forty-one engorged females were captured, dissected and the gut content adsorbed on a whatman filter paper and analysed using the enzyme-linked immunosorbent assay (elisa) technique to determinate the bloodmeal origin. results indicated that ae v. ar ...201121790686
Environmental factors associated with the distribution of floodwater mosquito eggs in irrigated fields in Wroclaw, Poland.A survey of distribution patterns of floodwater mosquito eggs related to environmental conditions such as moisture and plant associations was conducted by using soil samples from irrigated fields in Wroclaw, Poland. Mosquito egg distribution was determined by repeatedly flooding the soil samples with aerated water at a temperature of 25° C. Under laboratory conditions, hatching in installments of Aedes caspius (Pallas) and Aedes vexans (Meigen) were commonly observed. The results show that ~75% ...201122129404
clinical aspects of dermatitis associated with dirofilaria repens in pets: a review of 100 canine and 31 feline cases (1990-2010) and a report of a new clinic case imported from italy to dubai.cutaneous dirofilariasis is a parasitic disease caused by the mosquito-borne filarial nematodes dirofilaria (nochtiella) repens, living in the subcutaneous tissue of dogs, cats, wild carnivores, and humans. cases have been recently reported also from germany, czech republic, hungary, ukraine, russia, austria, switzerland, france, the netherlands, and the middle east. d. repens is not widely known to cause chronic pruritic dermatitis in animals. dermatological signs observed in 100 canine clinic ...201122203888
Spatio-temporal patterns of distribution of West Nile virus vectors in eastern Piedmont Region, Italy.ABSTRACT: BACKGROUND: West Nile Virus (WNV) transmission in Italy was first reported in 1998 as an equine outbreak near the swamps of Padule di Fucecchio, Tuscany. No other cases were identified during the following decade until 2008, when horse and human outbreaks were reported in Emilia Romagna, North Italy. Since then, WNV outbreaks have occurred annually, spreading from their initial northern foci throughout the country. Following the outbreak in 1998 the Italian public health authority def ...201122152822
the status of rift valley fever in animals in saudi arabia: a mini review.in august-september 2000, an overwhelming outbreak of rift valley fever (rvf) struck the southwestern part of saudi arabia and adjoining yemeni territories. during the outbreak, which was the first ever to be recorded outside africa, around 40,000 animals, mostly sheep and goats, died or aborted and 883 cases, with 124 deaths, were recorded among humans in saudi arabia. an additional 1328 human cases, with 166 deaths, were concurrently recorded in northwestern yemen. vector studies in saudi arab ...201121923257
temporal and spatial patterns of west nile virus transmission in saginaw county, michigan, 2003-2006.the dynamics of west nile virus (family flaviviridae, genus flavivirus, wnv) infection in mosquitoes, sentinel pheasants, and wild dead birds were evaluated during 2003-2006 in saginaw co., mi. mosquitoes were collected by new jersey light traps at 22 sites during may-september, pooled by species and sample location, and tested for presence of wnv rna by using a real-time reverse transcription-polymerase chain reaction assay. oral swabs from wild dead birds submitted by the public were tested by ...201121936324
the centers for disease control and prevention resting trap: a novel device for collecting resting mosquitoes.commercially available wood-fiber pots used to collect resting mosquitoes were modified to improve sampling efficiency. the modified traps, called the centers for disease control and prevention resting traps, collected 16.0 and 5.2 times more adult culex pipiens and cx. tarsalis than the conventional wood-fiber pots. the resting trap increases the mean number of resting mosquitoes collected per trap-night and is useful for collecting blood-engorged mosquitoes.201122017100
Longevity and efficacy of bifenthrin treatment on desert-pattern US military camouflage netting against mosquitoes in a hot-arid environment.The current Department of Defense pest management system does not provide adequate protection from arthropod disease vectors to personnel deployed in support of US military operations. We hypothesized that military camouflage netting, ubiquitous around living and working areas in current US military operations in Africa and the Middle East, treated with a residual pesticide such as bifenthrin may reduce the presence of biting insects and improve the military pest management system. In this study ...201122017092
Prevalence of toscana and sicilian phlebovirus antibodies in classic Kaposi sarcoma case patients and control subjects in sicily.To assess whether arthropod bites promote Kaposi sarcoma (KS), we determined the seroprevalence of Sicilian (SFSV) and Toscana (TOSV) phlebovirus antibodies in 30 patients with classic KS and 100 controls in Sicily. Nine (6.9%) subjects, all controls, were positive for SFSV, whereas 41 (31.5%) were positive for TOSV. Seroprevalence with immunoglobulin (Ig) M or IgG against either virus was significantly higher in controls (43% vs 13.3% in case patients; P < .01). Adjusted for age, IgG seropreval ...201121900487
Isolation of usutu virus in Germany.Usutu virus (USUV) is a mosquito-borne flavivirus that emerged 2001 in Austria and caused deaths in wild birds. In Germany, 70,378 female mosquitoes were captured in 2009 and 2010 and assayed for USUV. Virus was isolated in cell culture from one pool of Culex pipiens pipiens mosquitoes trapped exclusively in August 2010 in Weinheim, Germany. Subsequent phylogenetic analysis demonstrated a close relationship between the isolated USUV strain from Germany and a USUV strain from Austria, which was d ...201121896821
Competency of reptiles and amphibians for eastern equine encephalitis virus.Eastern equine encephalitis virus (EEEV) is endemic throughout most of the eastern United States. Although it is transmitted year round in Florida, transmission elsewhere is seasonal. The mechanism that enables EEEV to overwinter in seasonal foci remains obscure. In previous field studies, early season EEEV activity was detected in mosquito species that feed primarily upon ectothermic hosts, suggesting that reptiles and amphibians might represent overwintering reservoir hosts for EEEV. To determ ...201121896798
Mosquito species composition and Plasmodium vivax infection rates on Baengnyeong-do (island), Republic of Korea.Vivax malaria is a significant military and civilian health threat in the north of the Republic of Korea (ROK). The island of Baengnyeong-do is the westernmost point of the ROK and is located close to the southwestern coast of the Democratic People's Republic of Korea (DPRK). Mosquitoes were collected using a black light trap on Baengnyeong-do, and Anopheles spp. were assayed by PCR, to identify the species, and screened for sporozoites of Plasmodium vivax. Of a subsample of 257 mosquitoes, Anop ...201122072836
circulation of diverse genotypes of tahyna virus in xinjiang, people's republic of china.tahyna virus (tahv) is widely distributed in europe and asia. a previous study reported a high level of conservation of the tahv genome in isolates from europe. during 2006 and 2007, three tahyna virus isolates from mosquitoes were obtained from various locations in xinjiang, people's republic of china. we analyzed the complete coding sequence of full-length small, medium, and large segments of these isolates. molecular and phylogenetic analyses of the three complete tahv genomes showed that seq ...201121896801
implication of haematophagous arthropod salivary proteins in host-vector interactions.the saliva of haematophagous arthropods contains an array of anti-haemostatic, anti-inflammatory and immunomodulatory molecules that contribute to the success of the blood meal. the saliva of haematophagous arthropods is also involved in the transmission and the establishment of pathogens in the host and in allergic responses. this survey provides a comprehensive overview of the pharmacological activity and immunogenic properties of the main salivary proteins characterised in various haematophag ...201121951834
nationwide surveillance of west nile virus targeting mosquitoes and dead birds from april 2004 through march 2007 in japan.we conducted nationwide west nile virus (wnv) surveillance targeting mosquitoes and dead birds to reveal whether the virus and its potential vectors are present in japan. a total of 12 766 mosquitoes and 230 dead birds were collected in april 2004-march 2005 (the 2004-2005 period), 10 755 mosquitoes and 267 dead birds in april 2005-march 2006 (the 2005-2006 period), and 8624 mosquitoes and 245 dead birds in april 2006-march 2007 (the 2006-2007 period). the species of most of the mosquitoes colle ...201120163573
rapid molecular detection methods for arboviruses of livestock of importance to northern europe.arthropod-borne viruses (arboviruses) have been responsible for some of the most explosive epidemics of emerging infectious diseases over the past decade. their impact on both human and livestock populations has been dramatic. the early detection either through surveillance or diagnosis of virus will be a critical feature in responding and resolving the emergence of such epidemics in the future. although some of the most important emerging arboviruses are human pathogens, this paper aims to high ...201222219660
a diagnostic algorithm to serologically differentiate west nile virus from japanese encephalitis virus infections and its validation in field surveillance of poultry and horses.abstract the detection of west nile virus (wnv) in areas endemic for japanese encephalitis virus (jev) is complicated by the extensive serological cross-reactivity between the two viruses. a testing algorithm was developed and employed for the detection of anti-wnv antibody in areas endemic for jev. using this differentiation algorithm, a serological survey of poultry (2004 through 2009) and horses (2007 through 2009) was performed. among 2681 poultry sera, 125 samples were interpreted as bein ...201222217162
survey of mosquito fauna in san antonio, texas.a faunal survey of mosquitoes was conducted in 2008, 2009, and 2010 at various trapping locations throughout the city of san antonio, tx. a total of 22,739 mosquitoes representing 41 species in 8 genera were collected during this study. the most prevalent mosquito species collected were aedes vexans (25%), ae. sollicitans (14%), culex tarsalis (13.5%), and cx. erraticus (11%). four mosquito species (ae. bimaculatus, anopheles freeborni, cx. declarator, and psorophora horrida) not previously reco ...201223833905
re-emergence of rift valley fever virus in barkedji (senegal, west africa) in 2002-2003: identification of new vectors and epidemiological implications.the rift valley fever virus (rvfv) is a threat that must not be neglected, as the consequences of rvfv are dramatic, both for human and animal health. this virus is a zoonotic virus that already has demonstrated a real capacity for re-emerging after long periods of silence, as observed in barkedji (senegal, west africa) in 2002. in this article we present the 2nd emergence in barkedji after the 1st manifestation in 1993, and for the 1st time the circulation of rvfv during 2 consecutive years amo ...201223833896
theoretical potential of passerine filariasis to enhance the enzootic transmission of west nile virus.vertebrate reservoirs of arboviruses are often infected with microfilariae (mf). laboratory studies have shown that mf can enhance the infectivity of arboviruses to mosquitoes. soon after being ingested, mf penetrate the mosquito midgut. if the host blood also contains virus (i.e., vertebrate is dually infected), penetrating mf may introduce virus into the hemocoel. this can transform otherwise virus-incompetent mosquito species into virus-competent species and simultaneously accelerate viral de ...201223270173
combining hydrology and mosquito population models to identify the drivers of rift valley fever emergence in semi-arid regions of west africa.rift valley fever (rvf) is a vector-borne viral zoonosis of increasing global importance. rvf virus (rvfv) is transmitted either through exposure to infected animals or through bites from different species of infected mosquitoes, mainly of aedes and culex genera. these mosquitoes are very sensitive to environmental conditions, which may determine their presence, biology, and abundance. in east africa, rvf outbreaks are known to be closely associated with heavy rainfall events, unlike in the semi ...201222928055
effects of co-habitation between anopheles gambiae s.s. and culex quinquefasciatus aquatic stages on life history traits.the effective measures for the control of malaria and filariasis vectors can be achieved by targeting immature stages of anopheline and culicine mosquitoes in productive habitat. to design this strategy, the mechanisms (like biotic interactions with conspecifc and heterospecific larvae) regulating mosquito aquatic stages survivorship, development time and the size of emerging adults should be understood. this study explored the effect of co-habitation between an. gambiae s.s. and cx. quinquefasc ...201222321562
impact of mosquito bites on asexual parasite density and gametocyte prevalence in asymptomatic chronic plasmodium falciparum infections and correlation with ige and igg titers.an immunomodulatory role of arthropod saliva has been well documented, but evidence for an effect on plasmodium sp. infectiousness remains controversial. mosquito saliva may orient the immune response toward a th2 profile, thereby priming a th2 response against subsequent antigens, including plasmodium. orientation toward a th1 versus a th2 profile promotes igg and ige proliferation, respectively, where the former is crucial for the development of an efficient antiparasite immune response. here ...201222451520
identifying the main mosquito species in china based on dna barcoding.mosquitoes are insects of the diptera, nematocera, and culicidae families, some species of which are important disease vectors. identifying mosquito species based on morphological characteristics is difficult, particularly the identification of specimens collected in the field as part of disease surveillance programs. because of this difficulty, we constructed dna barcodes of the cytochrome c oxidase subunit 1, the coi gene, for the more common mosquito species in china, including the major dise ...201223071708
molecular detection of setaria tundra (nematoda: filarioidea) and an unidentified filarial species in mosquitoes in germany.knowledge of the potential vector role of culicidae mosquitoes in germany is very scanty, and until recently it was generally assumed that they are not involved in the transmission of anthroponotic or zoonotic pathogens in this country. however, anticipated changes in the course of global warming and globalization may alter their status.201222236560
key factors influencing canine heartworm, dirofilaria immitis, in the united states.an examination of the companion animal parasite council's (capc) canine heartworm data to clarify the spatial prevalence of heartworm in the united states. factors thought to influence the spatial risk of disease, as identified in a recent capc workshop, are discussed.201223111089
mosquito species composition and plasmodium vivax infection rates for korean army bases near the demilitarized zone in the republic of korea, 2011.vivax malaria is a significant military and civilian health threat in northern republic of korea (rok). mosquito collections were performed at two rok army installations, paju near the demilitarized zone (dmz) using black light traps in 2011. the dmz, a 4 km wide border, is the northernmost point of the rok and separates the rok from the democratic people's republic of korea (dprk). anopheles spp. were identified by polymerase chain reaction and screened for plasmodium vivax sporozoites. of 4,35 ...201223243112
an epizootic of eastern equine encephalitis virus, maine, usa in 2009: outbreak description and entomological studies.from july to september, 2009, an outbreak of eastern equine encephalitis virus (eeev) occurred in five counties in maine. the virus was isolated from 15 horses, 1 llama, and pheasants in three separate captive flocks. one wild turkey, screened before translocation, also showed exposure to the virus in january 2010. two pools of culiseta melanura (coquillett) tested positive for eeev during routine seasonal surveillance in york county in september, but none of the mosquitoes collected during rapi ...201223208877
development of a multi-target taqman assay to detect eastern equine encephalitis virus variants in mosquitoes.disease outbreaks caused by eastern equine encephalitis virus (eeev; togaviridae, alphavirus) may be prevented by implementing effective surveillance and intervention strategies directed against the mosquito vector. methods for eeev detection in mosquitoes include a real-time reverse transcriptase pcr technique (taqman assay), but we report its failure to detect variants isolated in connecticut in 2011, due to a single base-pair mismatch in the probe-binding site. to improve the molecular detect ...201222835151
analysis and optimization of a synthetic milkweed floral attractant for mosquitoes.a pentane extract of flowers of common milkweed, asclepias syriaca (asclepiadaceae), elicited significant orientation from both male and female culex pipiens in a dual-port flight olfactometer. analysis of the extract by gas chromatography-mass spectrometry revealed six major constituents in order of relative abundance: benzaldehyde, (e)-β-ocimene, phenylacetaldehyde, benzyl alcohol, nonanal, and (e)-2-nonenal. although not all were collected from the headspace profile of live flowers, a synthet ...201222711028
molecular xenomonitoring of dirofilaria immitis and dirofilaria repens in mosquitoes from north-eastern italy by real-time pcr coupled with melting curve analysis.dirofilaria immitis and dirofilaria repens are transmitted by bloodsucking culicid mosquitoes belonging to culex, aedes, ochlerotatus, anopheles and mansonia genera. the detection of filarioids in mosquitoes for assessing distribution of vectors and/or of pathogens in a given area (also known as "xenomonitoring"), when based on individual dissection of wild-caught female mosquitoes is time consuming and hardly applicable in large epidemiological surveys. our study aimed to evaluate the recently ...201222520170
north american wetlands and mosquito control.wetlands are valuable habitats that provide important social, economic, and ecological services such as flood control, water quality improvement, carbon sequestration, pollutant removal, and primary/secondary production export to terrestrial and aquatic food chains. there is disagreement about the need for mosquito control in wetlands and about the techniques utilized for mosquito abatement and their impacts upon wetlands ecosystems. mosquito control in wetlands is a complex issue influenced by ...201223222252
japanese encephalitis virus in mosquitoes and swine in yunnan province, china 2009-2010.the residential regions of yunnan province, canton of jing hong, in china were surveyed for japanese encephalitis virus (jev) infection in mosquito and swine vectors to determine the frequency of jev-carrying zoonotic vectors in 2009-2010. a total of 21,500 mosquitoes were collected and divided by species, and brain tissue was collected from 108 stillborn piglets. the infection rates for the different jev species were 13.2% for culex tritaeniorhynchus, 2.7% for anopheles sinensis, 0.7% for armig ...201223199264
mosquito, bird and human surveillance of west nile and usutu viruses in emilia-romagna region (italy) in 2010.in 2008, after the first west nile virus (wnv) detection in the emilia-romagna region, a surveillance system, including mosquito- and bird-based surveillance, was established to evaluate the virus presence. surveillance was improved in following years by extending the monitoring to larger areas and increasing the numbers of mosquitoes and birds tested.201222666446
detection of mosquito-only flaviviruses in europe.the genus flavivirus, family flaviviridae, includes a number of important arthropod-transmitted human pathogens such as dengue viruses, west nile virus, japanese encephalitis virus and yellow fever virus. in addition, the genus includes flaviviruses without a known vertebrate reservoir, which have been detected only in insects, particularly in mosquitoes, such as cell fusing agent virus, kamiti river virus, culex flavivirus, aedes flavivirus, quang binh virus, nakiwogo virus and calbertado virus ...201222377581
cross-correlation map analyses show weather variation influences on mosquito abundance patterns in saginaw county, michigan, 1989-2005.weather is important determinant of mosquito abundance that, in turn, influences vectorborne disease dynamics. in temperate regions, transmission generally is seasonal as mosquito abundance and behavior varies with temperature, precipitation, and other meteorological factors. we investigated how such factors affected species-specific mosquito abundance patterns in saginaw county, mi, during a 17-yr period. systematic sampling was undertaken at 22 trapping sites from may to september, during 1989 ...201222897045
detection and molecular characterization of avian plasmodium from mosquitoes in central turkey.assessing vector-parasite relationship is important in understanding the emergence of vector-borne diseases and the evolution of parasite diversity. this study investigates avian plasmodium parasites in mosquitoes collected from kayseri province in central anatolian, turkey and determines the haemosporidian parasite lineages from these mosquito species. a total of 6153 female mosquitos from 6 species were collected from 46 sites during june-august of 2008 and 2009. each mosquito's head-thorax an ...201222455723
equine seroprevalence rates as an additional indicator for a more accurate risk assessment of the west nile virus transmission.the west nile virus (wnv) is a zoonotic arbovirus that has recently been causing outbreaks in many countries in southern and central europe. in 2012, for the first time, it caused an outbreak in eastern croatia with total of 7 human clinical cases. with an aim of assisting public health personnel in order to improve survey protocols and vector control, the high risk areas of the wnv transmission were estimated and mapped. the study area included cities of osijek and slavonski brod and 8 municipa ...201324308242
potential for mosquitoes (diptera: culicidae) from florida to transmit rift valley fever virus.we evaluated aedes atlanticus dyar and knab, aedes infirmatus dyar and knab, aedes vexans (meigen), anopheles crucians wiedemann, coquillettidia perturbans (walker), culex nigripalpus theobald, mansonia dyari belkin, heinemann, and page, and psorophora ferox (von humboldt) from florida to determine which of these species should be targeted for control should rift valley fever virus (rvfv) be detected in north america. female mosquitoes that had fed on adult hamsters inoculated with rvfv were inc ...201324180117
prevalence of dirofilaria immitis (nematoda: filarioidea) in mosquitoes from northeast arkansas, the united states.a mosquito survey was conducted to identify which species of mosquitoes carry dirofilaria immitis (leidy) (nematoda: filarioidea), dog heartworm, in northeast arkansas. using polymerase chain reaction, mosquitoes were analyzed for d. immitis, dirofilaria repens railliet & henry, and acanthocheilonema dracunculoides cobbold. mosquitoes were collected from april to october 2009 using black light ultraviolet traps baited with dry ice. sixteen mosquito species were identified. d. immitis was identif ...201323926787
prevalence of dirofilaria immitis (spirurida: onchocercidae) infection in aedes, culex, and culiseta mosquitoes from north san joaquin valley, ca.canine heartworm is one of the most serious infections primarily affecting domestic dogs but will also infect cats and wild canids. to evaluate the potential of mosquitoes as vectors of dog heartworm, dirofilaria immitis (leidy) in san joaquin county, ca, we collected mosquitoes in 2011 and analyzed for infection with heartworm by using polymerase chain reaction. of 3,000 mosquito pools (total number of specimens = 36,554), d. immitis dna was detected in 97 pools of seven species, and the overal ...201324843938
evaluation of methods for collecting blood-engorged mosquitoes from habitats within a wildlife refuge.mortality of american white pelican (pelecanus erythrorhynchos) chicks attributed to west nile virus (wnv) prompted field studies on the bionomics of mosquitoes on a wildlife refuge in northern montana. one component of these studies was to identify blood meal sources for culex tarsalis, the primary vector of wnv in the region, and the potential bridge vectors aedes vexans and culiseta inornata. to accomplish this, 3 methods were evaluated to collect bloodfed mosquitoes: a gasoline powered aspir ...201323923324
geographic distribution of wolbachial infections in mosquitoes from thailand.members of the genus wolbachia are inherited intracellular bacterial endosymbionts that infect a diverse range of arthropods. here i report the results of a survey of these endosymbionts in different mosquito species from six geographic regions of northern, northeastern, western, central, eastern and southern thailand. using gene amplification assays with wsp and groe gene primers, wolbachiae were detected in 999 mosquitoes representing 28 species of 1622 specimens collected representing 74 spec ...201323660513
survey of new mosquito species of meghalaya, india.a study on mosquitoes in parts of meghalaya revealed the presence of 64 species of mosquitoes under 10 genera. in monsoon (june to october) and post-monsoon (november to may) survey, 42 and 48 species of mosquitoes were detected, respectivily. higher species diversity was found at lower elevations (up to 1050 m). anopheles peditaeniatus, an. sinensis, aedes vexans, ae. nigrostriatus, ae. annandalei, culex. fuscocephala, cx. pseudovishnui, cx. murrelli, cx. brevipalpis, coquillettidia novochracea ...201324620578
genetic diversity and differentiation between palearctic and nearctic populations of aedimorphus (=aedes) vexans (meigen, 1830) (diptera, culicidae).genetic diversity was studied at allozyme loci in two palearctic and one nearctic population of aedimorphus (=aedes) vexans, a species of public health and veterinary importance. the population from serbia was the most polymorphic (p= 35%) with the highest observed heterozygosity (h(o) = 0.027). the lowest observed heterozygosity (h(o) = 0.010) was obtained for the nearctic population. all analyses based on individual (structure analysis) and population level (pairwise f(st), nm values, amova, n ...201323701620
isolation and genomic characterization of chaoyang virus strain rok144 from aedes vexans nipponii from the republic of korea.during june 2003, mosquito surveillance was conducted at a us army installation and a us military training site 2 km south of the demilitarized zone, republic of korea. mosquitoes were collected using mosquito magnets™, sorted to species, and assayed for the presence of arboviruses. from the 3,149 mosquitoes that were sorted into 126 pools, one aedes vexans nipponii pool (out of 73 pools) tested positive for flavivirus rna by reverse transcription-pcr. after isolation from c6/36 cell culture sup ...201323127596
stage and age structured aedes vexans and culex pipiens (diptera: culicidae) climate-dependent matrix population model.aedes vexans and culex pipiens mosquitoes are potential vectors of many arbovirial diseases. due to the ongoing climate changes and reappearance of some zoonoses that were considered eradicated, there is a growing concern about potential disease outbreaks. therefore, the prediction of increased adult population abundances becomes an essential tool for the appropriate implementation of mosquito control strategies. in order to describe the population dynamics of a. vexans and c. pipiens mosquitoes ...201322971424
surveillance of japanese encephalitis virus infection in mosquitoes in vietnam from 2006 to 2008.japanese encephalitis virus (jev) infection in mosquitoes was monitored in vietnam from 2006 to 2008. a total of 15,225 mosquitoes, identified as 26 species in five genera were collected and 12,621 were grouped into 447 pools for examination of jev infection by assays for cytopathic effects in c6/36 cells and by rt-pcr to detect flavivirus rna. three jev strains were isolated from culex tritaeniorhynchus giles collected in northern and southern vietnam and two jev strains were isolated from cule ...201323358634
molecular detection and genotyping of japanese encephalitis virus in mosquitoes during a 2010 outbreak in the republic of korea.japanese encephalitis virus (jev), a mosquito-borne zoonotic pathogen, is one of the major causes of viral encephalitis. to reduce the impact of japanese encephalitis among children in the republic of korea (rok), the government established a mandatory vaccination program in 1967. through the efforts of this program only 0-7 (mean 2.1) cases of japanese encephalitis were reported annually in the rok during the period of 1984-2009. however, in 2010 there was an outbreak of 26 confirmed cases of j ...201323390520
recurrence of japanese encephalitis epidemic in wuhan, china, 2009-2010.japanese encephalitis (je) was once epidemic in most areas of china, including wuhan, a city located in the central part of china. the incidence of je dramatically decreased due to nationwide immunization with the live attenuated je virus (jev) vaccine, and no je cases were reported during 2005-2008 in wuhan. in 2009 and 2010, 31 je cases reoccurred in this area. in this study, we investigated the causes of je recurrence.201323326348
a video clip of the biting midge culicoides anophelis ingesting blood from an engorged anopheles mosquito in hainan, china.biting midges are hematophagus ectoparasites of insects, humans and other animals. culicoides (trithicoides) anophelis edwards1922 is a predator of engorged mosquitoes.201324499575
vector contact rates on eastern bluebird nestlings do not indicate west nile virus transmission in henrico county, virginia, usa.sensitive indicators of spatial and temporal variation in vector-host contact rates are critical to understanding the transmission and eventual prevention of arboviruses such as west nile virus (wnv). monitoring vector contact rates on particularly susceptible and perhaps more exposed avian nestlings may provide an advanced indication of local wnv amplification. to test this hypothesis we monitored wnv infection and vector contact rates among nestlings occupying nest boxes (primarily eastern blu ...201324287858
molecular identification of vertebrate and hemoparasite dna within mosquito blood meals from eastern north dakota.to understand local transmission of vector-borne diseases, it is important to identify potential vectors, characterize their host feeding patterns, and determine if vector-borne pathogens are circulating within the region. this study simultaneously investigated these aspects of disease transmission by collecting engorged mosquitoes within two rural study sites in the central red river valley of north dakota. mosquitoes were identified, midguts were excised, and the blood was expelled from the mi ...201324107213
european surveillance for west nile virus in mosquito populations.a wide range of arthropod-borne viruses threaten both human and animal health either through their presence in europe or through risk of introduction. prominent among these is west nile virus (wnv), primarily an avian virus, which has caused multiple outbreaks associated with human and equine mortality. endemic outbreaks of west nile fever have been reported in italy, greece, france, romania, hungary, russia and spain, with further spread expected. most outbreaks in western europe have been due ...201324157510
aedes mosquito saliva modulates rift valley fever virus pathogenicity.rift valley fever (rvf) is a severe mosquito-borne disease affecting humans and domestic ruminants. mosquito saliva contains compounds that counteract the hemostatic, inflammatory, and immune responses of the host. modulation of these defensive responses may facilitate virus infection. indeed, aedes mosquito saliva played a crucial role in the vector's capacity to effectively transfer arboviruses such as the cache valley and west nile viruses. the role of mosquito saliva in the transmission of r ...201323785528
avian hosts of west nile virus in arizona.west nile virus (wnv) causes sporadic outbreaks of human encephalitis in phoenix, arizona. to identify amplifying hosts of wnv in the phoenix area, we blood-sampled resident birds and measured antibody prevalence following an outbreak in the east valley of metropolitan phoenix during summer, 2010. house sparrow (passer domesticus), house finch (haemorhous mexicanus), great-tailed grackle (quiscalus mexicanus), and mourning dove (zenaida macroura) accounted for most wnv infections among locally r ...201323857022
emerging pathogens - how safe is blood?during the last few decades, blood safety efforts were mainly focused on preventing viral infections. however, humanity's increased mobility and improved migration pathways necessitate a global perspective regarding other transfusion-transmitted pathogens. this review focuses on the general infection risk of blood components for malaria, dengue virus, trypanosoma cruzi (chagas disease) and babesia spp. approximately 250 million people become infected by plasmodium spp. per year. dengue virus aff ...201324659943
west nile virus state of the art report of malwest project.during the last three years greece is experiencing the emergence of west nile virus (wnv) epidemics. within this framework, an integrated surveillance and control programme (malwest project) with thirteen associate partners was launched aiming to investigate the disease and suggest appropriate interventions. one out of seven work packages of the project is dedicated to the state of the art report for wnv. three expert working groups on humans, animals and mosquitoes were established. medical dat ...201324317379
francisella tularensis subspecies holarctica occurs in swedish mosquitoes, persists through the developmental stages of laboratory-infected mosquitoes and is transmissible during blood feeding.in sweden, mosquitoes are considered the major vectors of the bacterium francisella tularensis subsp. holarctica, which causes tularaemia. the aim of this study was to investigate whether mosquitoes acquire the bacterium as aquatic larvae and transmit the disease as adults. mosquitoes sampled in a swedish area where tularaemia is endemic (örebro) were positive for the presence of f. tularensis deoxyribonucleic acid throughout the summer. presence of the clinically relevant f. tularensis subsp. h ...201324057273
identification of hotspots in the european union for the introduction of four zoonotic arboviroses by live animal trade.live animal trade is considered a major mode of introduction of viruses from enzootic foci into disease-free areas. due to societal and behavioural changes, some wild animal species may nowadays be considered as pet species. the species diversity of animals involved in international trade is thus increasing. this could benefit pathogens that have a broad host range such as arboviruses. the objective of this study was to analyze the risk posed by live animal imports for the introduction, in the e ...201323894573
risk of canine and human exposure to dirofilaria immitis infected mosquitoes in endemic areas of italy.the occurrence of infections by dirofilaria immitis in canine and human populations depends on several factors linked to both the definitive and intermediate hosts. little data are available on the risk of human and dog exposure to d. immitis in endemic areas. data collected on dog- and human-bait traps in endemic areas of north-eastern italy were used to estimate the likelihood of a receptive host coming into contact with an infected vector.201323510597
vector-borne helminths of dogs and humans in europe.presently, 45% of the total human population of europe, as well as their domestic and companion animals, are exposed to the risk of vector-borne helminths (vbh) causing diseases. a plethora of intrinsic biological and extrinsic factors affect the relationship among helminths, vectors and animal hosts, in a constantly changing environment. although canine dirofilarioses by dirofilaria immitis and dirofilaria repens are key examples of the success of vbh spreading into non-endemic areas, another e ...201323324440
dirofilaria repens microfilariae in aedes vexans mosquitoes in slovakia.in this study, we screened field-caught mosquitoes for presence of dirofilaria spp. by using a polymerase chain reaction (pcr) assay. potential occurrence of dirofilaria repens and dirofilaria immitis microfilariae was examined in 3,600 mosquitoes of eight species (aedes vexans, aedes cinereus, aedes rossicus, culex pipiens, culiseta annulata, ochlerotatus sticticus, ochlerotatus cantans and ochlerotatus caspius) collected from five locations in two districts (kosice and trebisov) of eastern slo ...201323846240
evolutionary and plastic responses of freshwater invertebrates to climate change: realized patterns and future potential.we integrated the evidence for evolutionary and plastic trait changes in situ in response to climate change in freshwater invertebrates (aquatic insects and zooplankton). the synthesis on the trait changes in response to the expected reductions in hydroperiod and increases in salinity indicated little evidence for adaptive, plastic, and genetic trait changes and for local adaptation. with respect to responses to temperature, there are many studies on temporal trait changes in phenology and body ...201324454547
socio-economic impact of rift valley fever to pastoralists and agro pastoralists in arusha, manyara and morogoro regions in tanzania.rift valley fever (rvf) is a viral notifiable zoonotic disease primarily of domestic ruminants that causes significant socio-economic impacts. using the 2006-07 outbreak cases, this study aimed to establish the socio-economic impact of rvf and assessing knowledge, attitude and practice of livestock keepers towards controlling rvf in selected areas of tanzania. data were collected in arusha, manyara and morogoro regions using questionnaires, focus group discussions and in-depth interviews with ke ...201324255846
host compatibility rather than vector-host-encounter rate determines the host range of avian plasmodium parasites.blood-feeding arthropod vectors are responsible for transmitting many parasites between vertebrate hosts. while arthropod vectors often feed on limited subsets of potential host species, little is known about the extent to which this influences the distribution of vector-borne parasites in some systems. here, we test the hypothesis that different vector species structure parasite-host relationships by restricting access of certain parasites to a subset of available hosts. specifically, we invest ...201323595266
the transmission potential of rift valley fever virus among livestock in the netherlands: a modelling study.rift valley fever virus (rvfv) is a zoonotic vector-borne infection and causes a potentially severe disease. many mammals are susceptible to infection including important livestock species. although currently confined to africa and the near-east, this disease causes concern in countries in temperate climates where both hosts and potential vectors are present, such as the netherlands. currently, an assessment of the probability of an outbreak occurring in this country is missing. to evaluate the ...201323876054
identifying landscape features associated with rift valley fever virus transmission, ferlo region, senegal, using very high spatial resolution satellite imagery.dynamics of most of vector-borne diseases are strongly linked to global and local environmental changes. landscape changes are indicators of human activities or natural processes that are likely to modify the ecology of the diseases. here, a landscape approach developed at a local scale is proposed for extracting mosquito favourable biotopes, and for testing ecological parameters when identifying risk areas of rift valley fever (rvf) transmission. the study was carried out around barkedji villag ...201323452759
first detection of heartland virus (bunyaviridae: phlebovirus) from field collected arthropods.heartland virus (hrtv), the first pathogenic phlebovirus (family: bunyaviridae) discovered in the united states, was recently described from two missouri farmers. in 2012, we collected 56,428 ticks representing three species at 12 sites including both patients' farms. amblyomma americanum and dermacentor variabilis accounted for nearly all ticks collected. ten pools composed of deplete nymphs of a. americanum collected at a patient farm and a nearby conservation area were reverse transcription-p ...201323878186
modeling the impact of climate change on the dynamics of rift valley fever.a deterministic seir model of rift valley fever (rvf) with climate change parameters was considered to compute the basic reproduction number ℛ 0 and investigate the impact of temperature and precipitation on ℛ 0. to study the effect of model parameters to ℛ 0, sensitivity and elasticity analysis of ℛ 0 were performed. when temperature and precipitation effects are not considered, ℛ 0 is more sensitive to the expected number of infected aedes spp. due to one infected livestock and more elastic to ...201424795775
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