Publications

epidemiological investigation on subcutaneous nodule containing a live worm suspected to be dirofilaria repens infection in a woman from rural kerala, india.		0	24049370
capture-recapture method for estimating annual incidence of imported dengue, france, 2007-2010.	imported dengue cases pose the public health risk for local circulation in european areas, especially southeast france, where the aedes mosquito is established. using a capture-recapture method with chao's estimator, we estimated the annual incidence of dengue fever and the completeness of existing mandatory notification and laboratory network surveillance systems. during 2007-2010, >8,300 cases with laboratory evidence of recent dengue infection were diagnosed. of these cases, 4,500 occurred in ...	0	24188574
enveloped viruses disable innate immune responses in dendritic cells by direct activation of tam receptors.	upon activation by the ligands gas6 and protein s, tyro3/axl/mer (tam) receptor tyrosine kinases promote phagocytic clearance of apoptotic cells and downregulate immune responses initiated by toll-like receptors and type i interferons (ifns). many enveloped viruses display the phospholipid phosphatidylserine on their membranes, through which they bind gas6 and protein s and engage tam receptors. we find that ligand-coated viruses activate tam receptors on dendritic cells (dcs), dampen type i ifn ...	0	23954153
sex-specific response of a mosquito to parasites and crowding.	host-parasite interactions are significantly influenced by the sex of the host and the environment in which the host is found. sex-specific responses to parasite infection, however, may change according to the host environment. i examine the combined effect of parasite infection and crowding on males and females of the mosquito aedes albopictus. at a high larval density, infected males experienced a greater relative reduction in body size than did infected females, whereas the pattern was revers ...	0	15252979
gap junction channel gating.	over the last two decades, the view of gap junction (gj) channel gating has changed from one with gjs having a single transjunctional voltage-sensitive (v(j)-sensitive) gating mechanism to one with each hemichannel of a formed gj channel, as well as unapposed hemichannels, containing two, molecularly distinct gating mechanisms. these mechanisms are termed fast gating and slow or 'loop' gating. it appears that the fast gating mechanism is solely sensitive to v(j) and induces fast gating transitio ...	0	15033578
building scientific capacity in developing countries.		0	14710175
globalisation and the challenges to health systems.		0	12114243
voltage-dependent gating of single gap junction channels in an insect cell line.	de novo formation of cell pairs was used to examine the gating properties of single gap junction channels. two separate cells of an insect cell line (clone c6/36, derived from the mosquito aedes albopictus) were pushed against each other to provoke formation of gap junction channels. a dual voltage-clamp method was used to control the voltage gradient between the cells (vj) and measure the intercellular current (ij). the first sign of channel activity was apparent 4.7 min after cell contact. ste ...	0	7524710
frequencies of mitochondrial dna haplotypes in laboratory cage populations of the mosquito, aedes albopictus.	a laboratory cage experiment was undertaken to study changes over time in the frequencies of two mitochondrial dna (mtdna) haplotypes in the mosquito, aedes albopictus, under two conditions: bidirectionally compatible matings and unidirectionally incompatible matings. frequencies were monitored for 10 generations in three replicate cages for each of the two conditions above. in cages with bidirectionally compatible strains, changes in haplotype frequencies were nondirectional and neither haploty ...	0	1398054
electrical coupling between cells of the insect aedes albopictus.	1. cell pairs of an insect cell line (aedes albopictus, clone c6/36) were used to study the electrical properties of intercellular junctions. a double voltage-clamp approach was adopted to control the voltage gradient between the cells and measure the intracellular current flow. 2. determinations of junctional conductance (gj) revealed two types of intercellular contacts, gap junctions and cytoplasmic bridges. identification occurred by means of functional criteria, i.e. the dependency of gj on ...	0	1593470
sequences of the coding and flanking regions of the large ribosomal subunit rna gene of mosquito mitochondria.	we have sequenced a 1.6 kbp region of the mosquito (aedes albopictus) mitochondrial genome containing the large ribosomal subunit ("lsu") rna gene, and have located the ends of the gene by s1 protection analysis and by comparison with rna sequences. the gene is preceded by a trnaval gene and followed by genes for trnaieuuag (rather than trnaleuuaa, as in mammalian mitochondria) and an extended reading frame homologous to mammalian urf1. it is approximately 1335 residues long and is very low (17% ...	0	6093053
origin of the actinomycin d insensitive rna species in aedes albopictus cells.	in the presence of actinomycin d or a combination of actinomycin d and either camptothecin or alpha-amanatin. aedes albopictus cells synthesize a variety of single stranded rna species. these actinomycin d resistant species are ethidium bromide sensitive and they are present in the cell cytoplasm in an rnase resistant structure which has the sedimentation and buoyant density characteristics of mitochondria. twelve actinomycin d insensitive rna species can be detected by electrophoresis in 7m ure ...	0	652522
methylated constituents of aedes albopictus poly (a)-containing messenger rna.	poly (a)-containing mrna prepared from cultured mosquito (aedes albopictus) cells was found to contain methylated 5'-terminal "caps" as well as internal m6a residues. both type i [m7g(5')ppp(5')xmp] and type ii [m7g(5')ppp(5')xmpymp] caps were present, at molar ratio of ca five to one. all four common rna bases were represented in the second position (xm) of the caps, adenine being the most abundant and n6-methyladenine being absent. the four bases were also represented in the third position (ym ...	0	198748
plaque production by arboviruses in singh's aedes albopictus cells.	we report plaquing tests of 124 virus strains, mostly arboviruses of 21 serological groups, in singh's line of aedes albopictus cells. thirty of these plaqued: all were arboviruses of six groups and were known or presumed to be mosquito borne. failing to plaque were 86 strains of arboviruses, mostly tick borne, two strains of insect pathogens, and six animal viruses not classified as arboviruses. among mosquito-borne agents, plaquing ability appeared related to serological classification. califo ...	0	234160
phylogenomics and analysis of shared genes suggest a single transition to mutualism in wolbachia of nematodes.	wolbachia, endosymbiotic bacteria of the order rickettsiales, are widespread in arthropods but also present in nematodes. in arthropods, a and b supergroup wolbachia are generally associated with distortion of host reproduction. in filarial nematodes, including some human parasites, multiple lines of experimental evidence indicate that c and d supergroup wolbachia are essential for the survival of the host, and here the symbiotic relationship is considered mutualistic. the origin of this mutuali ...	0	23960254
alien insects in italy: comparing patterns from the regional to european level.	the introduction of species outside their native range contributes to the loss of biodiversity, alters the structure and functioning of ecosystems, and damages economy and human health. insects are one of the taxa with the highest frequency of introduction due to their high diversity, biological properties, and close association with human activities. here, the allodiversity of italian entomofauna was analyzed, with a focus on tuscany (central italy). a list of alien insects in tuscany is includ ...	0	24219427
molecular cloning and sequence analysis of a novel p450 gene encoding cyp345d3 from the red flour beetle, tribolium castaneum.	a novel cdna clone encoding a cytochrome p450 gene has been isolated from the insecticide-susceptible strain of the red flour beetle, tribolium castaneum (herbst) (coleoptera: tenebrionidae). the nucleotide sequence of the clone, designated cyp345d3, was determined. the cdna is 1554 bp in length and contains an open reading frame from base pairs 32 to 1513, encoding a protein of 493 amino acid residues and a predicted molecular weight of 57466 daltons. the putative protein contains the classic h ...	0	20331399
strategies to reduce mortality from bacterial sepsis in adults in developing countries.		0	18752342
pharmacology of ativisha, musta and their substitutes.	the ayurvedic literature during the medieval period suggests the use of musta (cyperus rotundus), a common weed, as a pratinidhi dravya (substitute) for ativisha (aconitum heterophyllum), an endangered species. contemporary ayurvedic practice also uses cryptocoryne spiralis, (known as naattu atividayam in south india) and nagaramusta (cyperus scariosus) as substitutes for ativisha and musta, respectively. this article reviews published literature on the pharmacology of the above four species. bo ...	0	26167002
resolution of a chikungunya outbreak in a prospective cohort, cebu, philippines, 2012-2014.		0	27649081
itaya virus, a novel orthobunyavirus associated with human febrile illness, peru.	our genetic analyses of uncharacterized bunyaviruses isolated in peru identified a possible reassortant virus containing small and large gene segment sequences closely related to the caraparu virus and a medium gene segment sequence potentially derived from an unidentified group c orthobunyavirus. neutralization tests confirmed serologic distinction among the newly identified virus and the prototype and caraparu strains. this virus, named itaya, was isolated in 1999 and 2006 from febrile patient ...	0	25898901
in reply.		0	23667395
identity elements for the aminoacylation of metazoan mitochondrial trna(arg) have been widely conserved throughout evolution and ensure the fidelity of the agr codon reassignment.	eumetazoan mitochondrial trnas possess structures (identity elements) that require the specific recognition by their cognate nuclear-encoded aminoacyl-trna synthetases. the aga (arginine) codon of the standard genetic code has been reassigned to serine/glycine/termination in eumetazoan organelles and is translated in some organisms by a mitochondrially encoded trna(ser)ucu. one mechanism to prevent mistranslation of the aga codon as arginine would require a set of trna identity elements distinct ...	0	25603118
dengue induces platelet activation, mitochondrial dysfunction and cell death through mechanisms that involve dc-sign and caspases.	worldwide, dengue is the most prevalent human arbovirus disease. dengue infection may cause a range of clinical manifestations from self-limiting febrile illness through to a life-threatening syndrome accompanied by both bleeding and shock. thrombocytopenia is frequently observed in mild and severe disease; however, the mechanisms involved in denv-induced platelet activation and thrombocytopenia are incompletely understood.	0	23433144
feeding and breeding aspects of stomoxys calcitrans (diptera: muscidae) under laboratory conditions.	bionomic aspects of stomoxys calcitrans (linnaeus, 1758) (diptera: muscidae) were studied under laboratory conditions. for this reason, laboratory-rearing techniques were optimized at the national veterinary school of toulouse. the colony was maintained at 25±2°c, 50±10% rh under a 12-hour light cycle and observed daily. the size of each adult cage is 30x30x30 cm and designed to house about 500-1,000 flies. the average cycle from egg to adult was 19.2±1.7 days. the mean longevity of imagos was 9 ...	0	23193515
climatic similarity and biological exchange in the worldwide airline transportation network.	recent increases in the rates of biological invasion and spread of infectious diseases have been linked to the continued expansion of the worldwide airline transportation network (wan). here, the global structure of the wan is analysed in terms of climatic similarity to illuminate the risk of deliberate or accidental movements of climatically sensitive organisms around the world. from over 44,000 flight routes, we show, for each month of an average year, (i) those scheduled routes that link the ...	0	17426013
problems with mitochondrial dna as a marker in population, phylogeographic and phylogenetic studies: the effects of inherited symbionts.	mitochondrial dna (mtdna) has been a marker of choice for reconstructing historical patterns of population demography, admixture, biogeography and speciation. however, it has recently been suggested that the pervasive nature of direct and indirect selection on this molecule renders any conclusion derived from it ambiguous. we review here the evidence for indirect selection on mtdna in arthropods arising from linkage disequilibrium with maternally inherited symbionts. we note first that these sym ...	0	16048766
dengue risk among visitors to hawaii during an outbreak.	despite the high rates of dengue in many tropical destinations frequented by tourists, limited information is available on the risk for infection among short-term visitors. we retrospectively surveyed 4,000 persons who arrived in hawaii during the peak of the 2001-2002 dengue outbreak and collected follow-up serologic test results for those reporting denguelike illness. of 3,064 visitors who responded, 94 (3%) experienced a denguelike illness either during their trip or within 14 days of departu ...	0	15890133
an outbreak of chikungunya in jamshedpur, jharkhand in 2011.		0	23287142
rickettsia felis in aedes albopictus mosquitoes, libreville, gabon.		0	23017437
in vitro screening antibacterial activity of bidens pilosa linné and annona crassiflora mart. against oxacillin resistant staphylococcus aureus (orsa) from the aerial environment at the dental clinic.	currently multiresistant staphylococcus aureus is one common cause of infections with high rates of morbidity and mortality worldwide, which directs scientific endeavors in search for novel antimicrobials. in this study, nine extracts from bidens pilosa (root, stem, flower and leaves) and annona crassiflora (rind fruit, stem, leaves, seed and pulp) were obtained with ethanol: water (7:3, v/v) and their in vitro antibacterial activity evaluated through both the agar diffusion and broth microdilut ...	0	25076435
random amplification and pyrosequencing for identification of novel viral genome sequences.	ssrna viruses have high levels of genomic divergence, which can lead to difficulty in genomic characterization of new viruses using traditional pcr amplification and sequencing methods. in this study, random reverse transcription, anchored random pcr amplification, and high-throughput pyrosequencing were used to identify orthobunyavirus sequences from total rna extracted from viral cultures of acute febrile illness specimens. draft genome sequence for the orthobunyavirus l segment was assembled ...	0	22468136
lessons learned during dengue outbreaks in the united states, 2001-2011.	since 2001, three autochthonous dengue fever outbreaks have occurred in the united states: in hawaii (2001); brownsville, texas (2005); and southern florida (2009-2011). we sought to characterize and describe the response to these outbreaks from the perspectives of public health and vector control officials. by conducting a medical literature review through pubmed and news media searches through google, we identified persons involved in managing each outbreak; 26 persons then participated in qua ...	0	22469195
chikungunya in the caribbean--threat for europe.		0	25062125
the dispersal of aedes albopictus in the territory of hawaii.		1946	20996630
relative susceptibility of aëdes aegypti, aëdes albopictus, aëdes canadensis and anopheles quadrimaculatus to plasmodium gallinaceum.		1949	18137143
biological observations on the mosquito, aedes albopictus.		1954	14348973
susceptibility of aedes albopictus to dirofilaria repens infection in cats.		1961	13727536
a study of filaria in taiwan monkeys.	filarial adult were found in the intermuscular connective tissue of the peritracheal and mandibular region of the taiwan monkey(macaca cyclopis). the microfilariae in the peripheral blood demonstrated a marked nocturnal periodicity with a peak between 2100-0100 hours and a low diurnal minimum between 900 and 1300 hours. staining the microfilaria by various stains demonstrated differences in size. no development of microfilariae was detected in the culex fatigans, culex tritaeniohyncus, aedes alb ...	1964	12913612
dengue type 2 virus in naturally infected aedes albopictus mosquitoes in singapore.	a strain of dengue type 2 virus has been isolated from aedes albopictus collected in singapore. this is the first report of a natural isolation of dengue virus from this species, which has long been suspected as a vector in nature.	1965	17747572
[resistance to insecticides in aedes aegypti l. and aedes albopictus in phnom-penh (cambodia)].		1966	5976658
low humidity water-retention ability in eretmapodites chrysogaster and aedes albopictus.		1967	4382866
experimental studies with chikungunya virus in aedes aegypti and aedes albopictus.		1967	4383887
observations on possible competitive displacement between populations of aedes aegypti linnaeus and aedes albopictus skuse in calcutta.	the possibility of competitive displacement in calcutta between aedes aegypti, a known vector of arboviruses, and a. albopictus, a suspected vector, was explored by general collections of immature stages from all types of breeding-places and by exposing oviposition traps in tenement houses, and gardens in urban, suburban, and rural environments. a. aegypti was predominant in houses and tenements in urban areas, but a. albopictus was not excluded. both species occurred in about equal densities in ...	1967	5301385
barriers to hybridization between aedes aegypti and aedes albopictus (diptera: culicidae).		1967	28556103
susceptibility of aedes albopictus and aedes aegypti cell lines to infection by arbo and other viruses.		1968	5680415
isolation of dengue viruses in aedes albopictus cell cultures.		1969	5307611
a study on the cytopathic effect of arboviruses on cultures from aedes albopictus cell line.		1969	5771694
colorado tick fever virus: growth in a mosquito cell line.	two strains of colorado tick fever virus grew in singh's aedes albopictus cells. in one of three experiments, virus growth continued for 7 weeks.	1969	5798247
loss of mouse virulence in chikungunya virus from the carrier culture of aedes albopictus cell line.		1969	5823163
susceptibility of the aedes albopictus and a. aegypti cell lines to infection with arboviruses.		1969	5787147
heterochromatin in cells derived from aedes albopictus (skuse) mosquito tissue culture.		1971	5124485
studies on the characterization of cells in aedes albopictus cell cultures.		1971	5092725
propagation of arboviruses in singh's aedes cell lines. i. growth of arboviruses in aedes albopictus and a. aegypti cell lines.		1971	5142320
infection of an aedes albopictus cell line with an arbovirus isolated from anopheline mosquitoes.		1971	4398590
aedes aegypti (l.) and aedes albopictus (skuse) in singapore city. 1. distribution and density.	the distribution and density of ae. aegypti and ae. albopictus in singapore were assessed from extensive larval surveys carried out from 1966 to 1968 to evaluate their respective roles in the epidemiology of dengue haemorrhagic fever and to study their ecology in the urban areas. ten urban areas where the majority of dengue haemorrhagic fever cases occurred were surveyed.the results showed that both species were common in the city, with ae. aegypti being the dominant species. the distribution of ...	1971	5316745
aedes aegypti (l.) and aedes albopictus (skuse) in singapore city. 2. larval habitats.	detailed information on the breeding habitats of ae. aegypti and ae. albopictus is necessary when planning programmes for their control. the larval habitats of the two species in 10 city areas were counted and classified according to type, frequency of occurrence, location, and function. of all the breeding habitats recorded 95% were domestic containers. the most common ae. aegypti breeding habitats were ant traps, earthenware jars, bowls, tanks, tin cans, and drums, ant traps being the most com ...	1971	5316746
aedes aegypti (l.) and aedes albopictus (skuse) in singapore city. 3. population fluctuations.	fluctuations in the adult ae. aegypti and ae. albopictus populations and their relationship to rainfall were studied by weekly collections from a number of stations in the city. aedes aegypti populations generally fluctuated with the rainfall, with multiple peaks, except in the middle of the year when there was no increase in rainfall at the time of the peak in population. it is suggested that other regulating factors, in addition to rainfall, also determine the fluctuations of this species. aed ...	1971	5316747
aedes aegypti (l.) and aedes albopictus (skuse) in singapore city. 4. competition between species.	there is a current belief stemming from statements made in the literature that ae. aegypti is displacing ae. albopictus in a number of cities of south-east asia and in calcutta, india. a critical review of these works showed that either the observations were inconclusive or the methods of collection were biased for one or the other species.extensive surveys of the larval habitats of the two species in singapore showed that the sharing of breeding habitats was uncommon in both urban and rural are ...	1971	5316748
aedes aegypti (l.) and aedes albopictus (skuse) in singapore city. 5. observations in relation to dengue haemorrhagic fever.	dengue haemorrhagic fever in singapore was a disease of the urban human population, with concentrations of cases occurring in areas of high population density. mosquito surveys revealed that these areas also had high population densities of ae. aegypti and ae. albopictus.the disease occurred throughout the year but the incidence of cases appeared to follow a seasonal pattern. observations from 1966 to 1968 showed that the number of cases increased in april, reached a peak in november, and, there ...	1971	5316749
adaptation of singh's aedes albopictus (atc-15) cell line to media with calf and goat sera and their susceptibility to infection with some arboviruses.		1971	5103347
cross-insemination frequencies between strains of aedes albopictus and members of the aedes scutellaris group.		1971	5118866
3. rna of uninfected and sindbis virus-infected aedes albopictus cells.		1971	5142328
observations on the host range and feeding preferences of aedes albopictus (skuse).		1971	5153593
experimental infection of aedes aegypti and aedes albopictus with dengue viruses.		1971	5159140
meiosis in the male of culex pipiens and aedes albopictus and fertilization in the culex pipiens-complex.		1971	4398667
relative population densities of aedes albopictus and a. guamensis on guam.	aedes guamensis farner & bohart is indigenous to guam but a. albopictus is known to have arrived in 1944. the larval ecology of the two species is very similar; both species breed in tree holes, coconut shells, and other natural and man-made water containers. the purpose of the study was to compare the relative densities of larval populations of the two species with those determined in a survey made in 1948-49. the results suggest that as the population density of a. albopictus increased, that o ...	1972	4538192
chromosome characterization of three cell lines derived from aedes albopictus (skuse) and aedes aegypti (l.).		1972	4402377
propagation of 3 relatively solvent-resistant arboviruses in singh's aedes albopictus and a. aegypti cell lines.		1972	5036201
lipids of cultured mosquito cells (aedes albopictus). comparison with cultured mammalian fibroblasts (bhk 21 cells).		1973	4797240
[method of simultaneous mass production of larvae of aedes aegypti (l.) and aedes albopictus (skuse) diptera-culicidae].		1973	4773086
effect of amino acids on growths of singh's aedes albopictus cells and japanese encephalitis virus.		1973	4587473
morphological variants of sindbis virus obtained from infected mosquito tissue culture cells.	tissue-cultured aedes albopictus cells infected with morphologically homogeneous sindbis virus were found to produce progeny virions which could be divided into three classes based on size. the thickness of the envelope was constant on all three sizes of progeny virions suggesting that the variability in size rested with the viral nucleocapsid. it is suggested that the three classes of virions have icosahedral nucleocapsids composed of common subunits organized in decreasing triangulation number ...	1973	4128381
host-induced modification of dengue-2 virus surface antigens in aedes albopictus cells.		1973	4132537
susceptibility of aedes albopictus mosquito cell line to some arboviruses.		1973	4147144
replication of dengue virus type 2 in aedes albopictus cell culture.	the replication of type 2 dengue (d-2) virus in aedes albopictus (aal) mosquito cell cultures differed from that in vertebrate (llc-mk2) rhesus monkey kidney cells. virus readily replicated in aal cells at either 30 or 37 c, but had no apparent effect on the host cell. persistent infection was established with continual virus production for at least 6 months, although the virulence of progeny virus for both suckling mice and llc-mk2 cells became attenuated. density gradient analysis of infected ...	1973	4201188
singh's aedes albopictus cell cultures as helper cells for the adaptation of obodhiang and kotonkan viruses of the rabies serogroup to some vertebrat cell cultures.	multiplication of rabies serogroup viruses, obodhiang and kotonkan, was induced in vertebrate cell cultures by using singh's aedes albopictus cell cultures as "helper cells."	1973	4633481
studies of interferon production in aedes albopictus mosquito cells.		1973	4683227
prevalence of aedes aegypti and aedes albopictus and observations on the ecology of dengue haemorrhagic fever in several areas of thailand.		1973	4718121
induction of an interferon-like substance in persistently infected aedes albopictus cells.		1973	4735533
homologous viral interference in aedes albopictus cultures chronically infected with sindbis virus.	complete homologous interference is demonstrated in cultures of aedes albopictus cells chronically infected with sindbis virus. the interference occurred before there was any detectable rna synthesis by the superinfecting virus.	1973	4735592
purification of japanese encephalitis virus grown in bhk21 and singh's aedes albopictus cells by polyethylene glycol precipitation.		1973	4738144
hybridization between aedes aegypti and aedes albopictus in malaysia.		1973	4749074
field and laboratory observations on landing and biting periodicities of aedes albopictus (skuse).		1973	4749076
aedes mascarensis macgregor on mauritius. i. ecology in relation to aedes albopictus (skuse) (diptera: culicidae).		1973	4779920
temperature-sensitive virus from aedes albopictus cells chronically infected with sindbis virus.	cultures of aedes albopictus cells persistently infected with wild-type sindbis virus (sv-w) give rise to small plaque-forming mutants which are also temperature sensitive. these mutants, designated sv-c, are neutralized by antiserum produced against sv-w. mutant ts clones were isolated from sv-c by plaque purification. after serial undiluted passage in bhk or mosquito cells, each of the clones gave rise to ts(+) revertants which, however, remained mutant with respect to plaque morphology. ninet ...	1974	4855741
protein kinase and phosphoproteins of vesicular stomatitis virus.	protein kinases of similar but not identical activity were found associated with vesicular stomatitis (vs) virions grown in mouse l cells, primary chicken embryo (ce) cells, and bhk-21 cells, as well as being present in vs virions grown in hela and aedes albopictus cells. the virion kinase preferentially phosphorylated the nucleocapsid ns protein in vitro and to a lesser extent the envelope m protein. other virion proteins were phosphorylated in vitro only after drastic detergent treatment. part ...	1974	4359419
semliki forest virus replication in cultured aedes albopictus cells: studies on the establishment of persistence.		1974	4610090
growth inhibition of japanese encephalitis virus by proline deficiency in singh's aedes albopictus cells.		1974	4611418
[viral specific rna synthesis in sindbis infected "aedes albopictus" mosquito cells i. - evidence for a 18s rna species in infected cells (author's transl)].		1974	4463758
[feeding behavior of aedes aegypti (l.) and aedes albopictus (skuse) females and factors influencing the number of eggs deposited by same].		1974	4450555
comparison of lipid composition of aedes aegypti and aedes albopictus cells obtained from logarithmic and stationary phases of growth.	the total lipids, total neutral lipids, and total phospholipids from aedes aegypti and aedes albopictus cells cultivated in vitro in a medium containing fetal calf serum were analyzed. the mosquito cells were harvested in the logarithmic and stationary phases of growth. the fatty acid profiles of the lipids showed differences during the aging of the cells but not betweeen species. there was an increase in chain elongation and unsaturation of the fatty acids in the stationary phase when compared ...	1975	1117810
failure of rubella virus to replicate in mosquitos.	rubella virus failed to replicate in mosquitoes (aedes albopictus and culex fatigans) following parenteral inoculation. the virus demonstrated in mosquitoes tested immediately after inoculation but was not detected in insects sampled 7, 14 and 21 days postinoculation. this experiment provides further evidence that rubella is not an arbovirus, but does not invalidate its classification as a togavirus.	1975	345
mosquito cells infected with vesicular stomatitis virus yield unsialylated virions of low infectivity.	vesicular stomatitis virus propagated in and released from aedes albopictus cells had the normal complement of viral proteins; the glycoprotein contained carbohydrate but no sialic acid. these virions had markedly reduced hemagglutinating activity and exhibited a very high ratio of physical particles to infectious virus. in vitro sialylation of vesicular stomatitis virions grown in mosquito cells resulted in a 100-fold increase in both infectivity and hemagglutination titers to levels approachin ...	1975	163913
morphogenesis of sindbis virus in cultured aedes albopictus cells.	cultured mosquito cells were found to produce sindbis virus nearly as efficiently as bhk-21 cells at 28 c. in virtually all of the cells observed in the electron microscope, virus morphogenesis was found to occur within complex vesicular structures which developed after viral infection. viral nucleocapsids were first seen in these vesicles and appeared to be enveloped within these structures. the process of envelopment within these inclusions differed in some respects from the process previously ...	1975	170422
small- and large-plaque variants of chikungunya virus in two vertebrate and seven invertebrate cell lines.	nine cells lines--bhk-21, vero, aedes albopictus, a. aegypti (monolayer and howwow vesicles), a. w-albus, a. vittatus, anopheles stephensi and culex quinquefasciatus--were infected with small- and large-plaque (sp, lp) variants of chikungunya virus. ross strain, and incubated at different temperatures. in the aedes (29 plus or minus 1 degrees c) and the vertebrate cell lines (36 degrees c), infectivity titers of extracellular virus rapidly reached a peak; cytopathic effect (cpe) occurred only in ...	1975	235192
growth of tahyna virus at low temperatures.	replication of two tahyna virus strains in the aedes albopictus mosquito cell line was studied in the temperature range from 6 to 28 degrees c. the virus grew in this temperature range; its replication rate was related to the temperature of incubation. at lower temperatures the virus titres increased more slowly and did not reach as high maximum values as at higher temperatures. short increase in incubation temperature resulted in a short increase in the titre of virus previously incubated at 10 ...	1975	241231
isolation of spotted fever group and wolbachia-like agents from field-collected materials by means of plaque formation in mammalian and mosquito cells.	three isolations from ticks (dermacentor occidentalis) of a rickettsia of the spotted fever group and 5 isolations from chipmunk (eutamias rugicaudus) blood of a wolbachia-like agent were obtained from plaques formed in singh's aedes albopictus (mosquito) and vero (african green monkey kidney) cell cultures. these organisms could not be isolated by injection of the infected ticks or blood into embryonated chicken eggs, guinea pigs, or voles (microtus pennsylvanicus), but fluid cultures of grace' ...	1975	241251
detection of west nile complement-fixing antigen in aedes albopictus cell cultures.		1975	52295
observations of aedes albopictus cell cultures persistently infected with sindbis virus.		1975	1072595