Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| detection of house dust mite allergens and immunoblot analysis in asthmatic children. | the mite allergen extracts (dermatophagoides pteronyssinus and d. farinae), were fractionated by polyacrylamide gel electrophoresis and autoradiography. the molecular weight of the most apparent proteins of d. pteronyssinus was between 27,000 and 35,000, and the protein components of d. farinae were in the 9-100-kilodalton range. specific ige antibodies directed against mite allergens were utilized as probes to detect the allergens. using immunoblot analysis and radiostained technique, d. pteron ... | 1988 | 3182582 |
| investigations of patients allergic to the house dust mite dermatophagoides pteronyssinus by crossed radioimmunoelectrophoresis using purified mite bodies and whole mite culture extracts. | sera of 20 patients allergic to the house dust mite dermatophagoides pteronyssinus were investigated by crossed radioimmunoelectrophoresis (crie) using extracts prepared from purified mite bodies (pmb) and whole mite culture (wmc). by crie, different allergen patterns and different numbers of allergens were detected in the two extracts. by summarizing the values of the crie patterns of the 20 patients' sera into allergograms, 6 out of 16 allergens in pmb and 5 out of 10 allergens in wmc extracts ... | 1988 | 3170005 |
| effect of house dust mite avoidance measures on adult atopic asthma. | twenty one adult patients with asthma, with positive skin test responses to the european house dust mite, dermatophagoides pteronyssinus, were randomly allocated to a control group or to a group applying house dust mite avoidance measures. these included an initial application of liquid nitrogen to mattresses and bedroom carpets to kill the live house dust mite population. histamine airway responsiveness, symptom scores, peak expiratory flow rates (pef), and house dust mite numbers were determin ... | 1988 | 3353895 |
| the major allergen of the house dust mite, dermatophagoides pteronyssinus, is synthesized and secreted into its alimentary canal. | the natural production of der pl, the major faecal allergen from the house dust mite (dermatophagoides pteronyssinus), was investigated. mite cultures grown on radiolabelled substrate were examined at intervals over a period of 21 days and incorporation of radiolabel into the mite proteins measured over the time course. mite proteins were extracted, purified by chromatofocussing and characterized by immunodiffusion, sds-page, electroblotting and autoradiography. the faecal allergen, der pl, did ... | 1988 | 3350609 |
| sequence analysis of cdna coding for a major house dust mite allergen, der p 1. homology with cysteine proteases. | a cdna clone coding for der p 1, a major allergen from the house dust mite dermatophagoides pteronyssinus, has been sequenced. it codes for a 222 residue mature protein with a derived molecular weight of 25,371 and contains 1 potential n-glycosylation site. in addition, the cdna appears to code for a 13 residue proregion, and an incomplete signal peptide. the deduced sequence shows a high degree of homology with animal and plant cysteine proteases, particularly in the region of the contact resid ... | 1988 | 3335830 |
| specific immunotherapy with a standardized dermatophagoides pteronyssinus extract. ii. prediction of efficacy of immunotherapy. | house dust mites of the species dermatophagoides pteronyssinus (dpt) represent one of the major allergens inducing asthma. however, a strict allergy to dpt is not always observed in dpt-allergic patients, since nonallergic and other allergic triggering factors often coexist. it was suggested that specific immunotherapy with house dust-mite extracts is more effective in children than in adults. a controlled study was undertaken in 215 dpt-allergic patients with asthma ranging in age from 3 to 72 ... | 1988 | 3204255 |
| development of an enzyme-linked immunosorbent assay for detection of ige antibodies specific to dermatophagoides pteronyssinus. | allergy to dermatophagoides pteronyssinus was determined in 61 rhinitis patients using prick test (pt), enzyme-immunoassay (eia) and enzyme-linked immunosorbent assay (elisa). a total of 43 patients tested positive with pt. forty six patients were positive when tested with eia and elisa. with pt as standard test, eia was found to have 83.7% sensitivity and 44.4% specificity; elisa had 81.4% sensitivity and 38.9% specificity. there was a linear relationship between absorbance values obtained by e ... | 1988 | 3146256 |
| dose response curve of allergen and histamine in skin prick tests. | twenty-six dermatophagoides pteronyssinus (d.pt.) sensitive subjects were skin prick tested in duplicate with 15 concentrations of d.pt. ranging from 0.0018 to 17.8 mg/ml, 15 concentrations of the major allergen of d.pt., antigen p1, ranging from 0.0002 to 1.88 mg/ml and 15 concentrations of histamine dihydrochloride solution ranging from 0.048 to 114.0 mg/ml. weal areas and concentrations were transformed by taking logs and linear and non-linear regression curves fitted, allowing for confoundin ... | 1988 | 3207181 |
| occurrence and subclass distribution of igg antibodies to dietary antigens in children with atopic dermatitis and in their mothers. | occurrence and subclass distribution of igg to alpha-lactalbumin (ala), beta-lactoglobulin (blg), and ovalbumin (oa) have been determined in 30 children with atopic dermatitis (ad) and in their mothers. thirty nonatopic (na) children and their mothers were studied as controls. total ige and ige to ala, blg, oa, dermatophagoides pteronyssinus, and lolium perenne were also investigated. the main purpose of this study was to determine whether in humans, as observed in animals, maternal allergen-spe ... | 1988 | 2459993 |
| population growth and developmental stages of the house dust mite, dermatophagoides pteronyssinus (acari: pyroglyphidae). | 1988 | 3193430 | |
| prospective study on immunologic changes induced by two different dermatophagoides pteronyssinus extracts prepared from whole mite culture and mite bodies. | twenty-four children with bronchial asthma and hypersensitivity to dermatophagoides pteronyssinus by skin titration, specific serum ige, leukocyte histamine release, and bronchial challenge test were selected for immunotherapy with two partially purified mite extracts. on the basis of age and allergen-specific ige responses, patients were randomly divided for treatment with either an extract prepared from whole mite culture (wmc) or from purified mite bodies (mb). the content of major allergens ... | 1988 | 3170985 |
| effective hyposensitization in allergic rhinitis using a potent partially purified extract of house dust mite. | thirty-eight adults with allergic rhinitis have been treated with a new partially purified extract of house dust mite (dermatophagoides pteronyssinus) in a double-blind placebo-controlled trial. patients were randomized to active (pharmalgen, d. pteronyssinus) and placebo (histamine) treatment by sensitivity to d. pteronyssinus on nasal challenge. in the actively treated group nasal symptoms, assessed by visual analogue score, improved (p less than 0.01), sensitivity on nasal challenge with alle ... | 1988 | 3069238 |
| modification of the late asthmatic reaction by hyposensitization in asthmatic children allergic to house dust mite (dermatophagoides pteronyssinus) or grass pollen. | the frequency and severity of the late asthmatic reaction (lar) was studied in asthmatic children allergic to house dust mite (hdm) or grass pollen (gp) with and without hyposensitization (hs). the four groups were comparable according to their severity of asthma. all children were allergic to hdm (dermatophagoides pteronyssinus) or gp according to history, skin testing and specific ige determination via the rast. the lar occurred less frequently (29% versus 73%) (p less than 0.001) and was less ... | 1988 | 3414914 |
| [the immune response specific to dermatophagoides pteronyssinus body antigen in asthmatics]. | 1988 | 3214287 | |
| guanine and mite allergenicity in house dust. | guanine is the major nitrogenous waste product in arachnids. it may serve as an indicator for allergenic mite faecal pellets. the present study assesses the correlation between the mit allergenicity of different house dust (hd) samples and their guanine content. guanine content in hd samples was evaluated either by the acarex-test or determined quantitatively, especially for in-vitro methods. using intradermal tests in selectively sensitized dermatophagoides pteronyssinus (dpt) patients, we eval ... | 1988 | 2458199 |
| isotype specific immunoglobulin responses to the house dust mite dermatophagoides pteronyssinus and the purified allergen der p 1 in asthmatic and control subjects from the eastern highlands of papua new guinea. | the igg, ige and iga antibody responses to the whole mite extract and a purified major mite allergen der p 1, in sera from asthmatic and age- and sex-matched control subjects from the south fore region of the eastern highlands of papua new guinea, have been studied. radio-allergosorbent studies showed that the majority of the asthmatics, in contrast to control subjects, produced ige to whole mite extract, and that der p 1 was a major allergen in this population with 88% of mite allergic asthmati ... | 1988 | 3396192 |
| modification of house dust mite allergens by monomethoxypolyethylene glycol. allergenicity measured by in vitro and in vivo methods. | in animal models, allergen modification by coupling to monomethoxypolyethylene glycol (mpeg) molecules can reduce allergenicity of the extract and makes the allergen capable of suppressing boosted ige response. to investigate in a human system the degree of attenuation implied by a mpeg modification of a house dust mite (dermatophagoides pteronyssinus) extract, 55 adults with asthma caused by house dust mites were tested by skin prick test (spt) and histamine release assay (hr). rast inhibition ... | 1988 | 2448247 |
| passive sensitization of basophil leukocytes from non-atopic adult by plasma from allergic children. | basophil leukocytes from a non-atopic donor, who responded well to anti-ige, were depleted of their native membrane-bound ige by acid treatment and passively sensitized with plasma containing either phleum pratense-, dermatophagoides pteronyssinus- or dog dander- specific ige obtained from 18 allergic children. histamine release was then performed on the passively sensitized cells and the results were compared with those of bronchial provocation test (bpt), allergen-specific serum ige (rast), sk ... | 1988 | 2449829 |
| skin tests and histamine release with p1-depleted dermatophagoides pteronyssinus body extracts and purified p1. | monoclonal antibodies were raised against p1 and dp x, two major allergens in dermatophagoides pteronyssinus extracts. the concentrations of ige antibodies to p1 and dp x in sera of mite-sensitive patients were determined in rast with immunosorbent-bound monoclonal antibodies used for insolubilization of the allergens. the major allergen-specific ige titers were compared with the total ige response against d. pteronyssinus. the results of these serologic assays confirmed studies in the literatur ... | 1988 | 2453544 |
| latex allergosorbent test (last): a new immunoassay for specific ige with latex particles. | we describe a two-step latex (lx) agglutination assay for the titration of specific anti-dermatophagoides pteronyssinus ige. the samples are first incubated with allergen-coated lx of 2.3 microns diameter. bound ige is digested by pepsin and then titrated by its agglutinating activity on 0.8 micron lx particles coated with antihuman fc epsilon rabbit f(ab')2. this latex allergosorbent test detects 100 pg of specific ige per milliliter and does not depend on the concentration of total ige. owing ... | 1988 | 2455743 |
| release of highly-sulphated glycosaminoglycans and histamine from human basophils. | a new assay for highly-sulphated glycosaminoglycans (gags) has been applied to the study of allergen (dermatophagoides pteronyssinus)-induced release of gag from human basophils. highly-sulphated gag, which was not heparin, was found only in basophil-containing cellular fractions of whole blood. its release, like that of histamine, was ca++-dependent. however, allergen-induced release of gag from the basophils of atopic individuals exhibited a different time-course and dose-response from that of ... | 1988 | 2456991 |
| role of igg4 in histamine release from human basophil leucocytes. i. sensitization of cells from normal donors. | several conflicting reports on the ability of igg4 to mediate type i allergic reactions have appeared lately. we have developed a model system for testing this possibility, using passive sensitization of basophil leucocytes from normal individuals. at first, the system was optimized with regard to donor cells and anti-igg4 antibody: among 3 normal individuals with cells showing high, medium and low responses to anti-ige, only the high responder showed a reproducible, but low response to anti-igg ... | 1988 | 2457556 |
| mhc class ii restriction specificity of cloned human t lymphocytes reactive with dermatophagoides farinae (house dust mite). | in this report the antigen and restriction specificity of human t-cell clones induced with dermatophagoides farinae (d. farinae) and isolated from an atopic individual with perennial rhinitis has been investigated. of the six clones analysed, four were species specific and two showed cross-reactivity for the closely related dermatophagoides pteronyssinus (d. pteronyssinus). inhibition of antigen-dependent proliferation by murine monoclonal antibodies directed against hla-d-region gene products r ... | 1988 | 2459050 |
| loratadine, an antihistamine, blocks antigen-and ionophore-induced leukotriene release from human lung in vitro. | some h1-antihistamines possess anti-allergic properties, and inhibit the immunological release of mediators including histamine and sulfiopeptide-leukotrienes (slow reacting substance of anaphylaxis) from lung. the effects of the antihistiamine loratadine, sch29851, on the release of leukotrienes and histamine from human lung fragments were measured, using the calcium ionophore a23187 and an extract of antigen from dermatophagoides pteronyssinus, house dust mite, (with passively sensitized lung) ... | 1988 | 2470117 |
| [biochemical structure and allergenic activity of dermatophagoid mites (dermatophagoides pteronyssinus)]. | 1988 | 2971865 | |
| correlation between serum ige and specific ige antibody titer to house dust mite in children with asthma. | using the enzyme-linked immunosorbent assay (elisa) for ige specific to mites, we evaluated the relationship between total serum ige levels and ige antibodies specific to mite in 58 asthmatic children. our results showed that there was a positive correlation between total serum ige and ige antibody specific to dermatophagoides pteronyssinus (r = 0.57; p less than 0.001) and dermatophagoides farinae (r = 0.59; p less than 0.001). there was also a significant correlation between d. pteronyssinus-s ... | 1988 | 3182578 |
| comparison between modified skin prick test with standardized allergen extracts and phazet. | skin prick tests (spts) represent one of the most common techniques of skin testing and are of great value, especially when standardized extracts are used. phazet is a puncture test involving needles coated with standardized allergens. phazet and spts have been compared in 130 subjects tested with six standardized allergen extracts: orchard-grass, olive, and mugwort pollens, cat dander, dermatophagoides pteronyssinus, and alternaria. patients have been carefully selected on the basis of a sugges ... | 1988 | 3192871 |
| hypersensitivity to wheat flour in bakers. | eleven bakers and 4 pastry cooks with hypersensitivity to wheat flour underwent skin tests to flours (wheat, barley, rye, oats), pollens (wheat, barley, rye, oats, grasses), mites (dermatophagoides pteronyssinus, dermatophagoides farinae), molds (alternaria, aspergillus) and weeds (parietaria judaica). the levels of serum immunoglobulins (igg, iga, igm and ige) were studied and the values of specific ige (rast) in the presence of wheat flour and the histamine release test for wheat flour, grass ... | 1988 | 3228051 |
| sensitivity to parietaria pollen in the southampton area as determined by skin-prick and rast tests. | the pollen of parietaria species is a well-recognized and important inhalant allergen in the mediterranean area. parietaria judaica (pellitory-of-the-wall) is native to the u.k., flowering from june to september, but is not usually considered to be of any clinical importance by u.k. allergists. we skin tested 62 patients with a clinical history of summer seasonal respiratory symptoms and a control group of 11 patients with perennial respiratory symptoms only. each was skin tested in duplicate wi ... | 1988 | 3242974 |
| human immune response to allergens of house dust mite, dermatophagoides pteronyssinus. iv. occurrence of natural autologous anti-idiotypic antibodies. | igg isolated from the plasma of seven individuals hypersensitive to the common house dust mite dermatophagoides pteronyssinus (dpt) was exhaustively adsorbed onto insolubilized dpt. the unbound fraction was found by radioimmunoassay to contain antibodies recognizing the variable region of both anti-dpt igg and ige antibodies. this recognition was idiotype (id)-specific as it persisted after passage over insolubilized polyclonal igg of unrelated specificity. most of these anti-id igg carried the ... | 1988 | 3257924 |
| optimal efficacy of a fungicide preparation, natamycin, in the control of the house-dust mite, dermatophagoides pteronyssinus. | the efficacy of a fungicidal preparation, natamycin, for the effective control of dermatophagoides pteronyssinus (trouessart), was determined at different concentrations on mattress-dust microsites, including ticking. one treatment with the commercially available natamycin concentration (tymasil) resulted in a 65% reduction of house-dust mite populations within 4 weeks of treatment. the acaricidal control could be proven despite the protective role of mattress fibres such as ticking. however, mi ... | 1988 | 3259916 |
| human immune response to allergens of house dust mite, dermatophagoides pteronyssinus. v. auto-anti-idiotypic antibody characterization and cross-reactivity. | from the serum of 10 allergic subjects we have prepared igg antibodies recognizing idiotopes carried by specific antibodies to dermatophagoides pteronyssinus (dpt) allergens, and studied cross-reactivity of anti-dpt igg bystander and antigen-binding site-associated idiotopes by latex agglutination assays. idiotopes of specific anti-dpt ige were evaluated by radioimmunoassays. depending on the assay, a binding or inhibition of more than 50%, as compared to the reactivity of specific antibodies wi ... | 1988 | 3261244 |
| house dust mites and vernal keratoconjunctivitis. | the possible causative relationship between exposure to house dust mite allergen (hdma) and symptoms of vernal keratoconjunctivitis (vkc) was investigated. vkc patients were evaluated for an average of 18 months using patient reports of symptoms, physicians' observations and assays of sera and tears for total and specific ige to 10 inhalant allergens common in israel, including hdma. tear and serum samples from a group of 8 patients reacted only with hdma, and were compared with the tear and ser ... | 1988 | 3262844 |
| improved diagnosis and treatment of allergic rhinitis by the use of nasal provocation tests. | a simple and safe method of nasal provocation for out-patients that requires no special equipment is described. a total of 2,645 cases were tested by nasal provocation tests. of the total, 1,175 were tested with dermatophagoides pteronyssinus, 825 with mixed grass pollens, 352 with plantain (plantago lanceolata), and 125 for cats and correlating nasal tests with skin tests yielded 80%, 90%, and 85%, respectively. immunotherapy for allergic rhinitis was only considered justifiable when the nasal ... | 1988 | 3337397 |
| probit analysis applied to the allergen dose-response curve: a method for epidemiologic surveys. | the degree of skin sensitivity to five common allergens (grass, dermatophagoides pteronyssinus, mugwort, birch, and parietaria) was determined by the threshold dilution technique in all the skin test reactors of a random sample of 295 schoolchildren (142 male and 153 female subjects, age range 11 to 14 years), and the frequency distribution of responders at each concentration was analyzed by probit analysis. the potency of each allergen was presented in terms of median effective dose (ed50), and ... | 1988 | 3339189 |
| antigenic analysis of household dust samples. | household dust samples from the homes of 106 allergy clinic patients in baltimore were analyzed for specific allergen content. dust mite antigen content was determined by enzyme-linked immunosorbent assays (elisa) specific for the major allergens of dermatophagoides pteronyssinus, d. farinae, and d. microceras. cat and dog antigen content were determined by elisa using antisera for fel d 1 (formerly cat allergen 1) and dog allergens 3 and 13, respectively. mold content was assessed by culture wi ... | 1988 | 3341627 |
| human immune response to allergens of house dust mite, dermatophagoides pteronyssinus. iii. cross-reactivity of bystander idiotopes on allergen-specific ige antibodies. | rabbits were inoculated with affinity-purified human antibodies to the common house dust mite dermatophagoides pteronyssinus (dpt) to produce anti-idiotypic (id) antibodies. the id carried by human ige antibodies were studied in a radioimmunoassay in which specific anti-dpt ige were first insolubilized on rabbit f(ab')2 fragments against human fc epsilon. in a single individual specific anti-dpt ige antibodies shared bystander idiotopes (i.e. idiotopes located outside the antigen-binding site) w ... | 1988 | 3345795 |
| mite antigen and allergen contents of house dust samples. | the house dust mite (dermatophagoides pteronyssinus) antigen and allergen contents were measured by enzyme-linked immunosorbent assay (elisa) with enzyme-labelled anti-human ige and anti-mite rabbit igg antibodies. antigen content was high in dust samples from homes of patients with allergy but not in samples from homes of patients with kawasaki disease or of normal control subjects. allergen content was high in dust samples from homes of kawasaki disease patients. however, the values overlapped ... | 1988 | 3364213 |
| the binding of mouse hybridoma and human ige antibodies to the major fecal allergen, der p i, of dermatophagoides pteronyssinus. relative binding site location and species specificity studied by solid-phase inhibition assays with radiolabeled antigen. | the relative binding site location and species specificity of 19 mouse hybridoma antibodies, produced in four laboratories, to dermatophagoides pteronyssinus major fecal allergen, der p i, was studied by using immobilized mab and inhibitions of radiolabeled ag binding. four mab groups were defined, within which 4, 6, 8, and 5 mab, respectively, cross-inhibited each other. five mab were members of both group 2 and 3, demonstrating a considerable overlap of epitopes between the corresponding antib ... | 1988 | 3372999 |
| the pattern of human late-phase skin reactions to extracts of aeroallergens. | the kinetics and dose response of cutaneous late phase reactions (lpr) (defined as greater than 5 mm induration 6 hours after intradermal challenge with allergen) were studied in 20 randomly selected subjects with atopic rhinitis. when subjects were challenged intradermally with 0.2 biologic unit (bu) of dermatophagoides pteronyssinus or grass pollen, wheal-and-flare reactions were elicited without subsequent lprs. with 30 bu, all subjects developed lprs, whereas 1 and 10 bu provoked lprs in 28% ... | 1988 | 3379223 |
| human immune response to allergens of house dust mite, dermatophagoides pteronyssinus. isotypic analysis of antibodies in atopic and non-atopic subjects. | specific antibodies isolated by immunoabsorption on four main insolubilized allergens from dermatophagoides pteronyssinus (dpt) had the following isotypic distribution: in 16 atopic patients, 52% igg, 40% igm, 8% iga, 0.1% ige and, in 12 non-atopic individuals, 48% igg, 46% igm, 6% iga, 0.03% ige. the ratios between geometric means of antibody values in each class (atopic vs non-atopics) were 2.4 for igg, 2.0 for igm, 2.8 for iga and 66.7 for ige. the amount of anti-dpt antibodies in igg subclas ... | 1988 | 3414913 |
| strong delayed-type hypersensitivity induced against house dust mite antigens in the mice. | the aim of this study was to assess the antigenicity of house dust mite antigens using delayed-type hypersensitivity (dth) responses in mice. the crude extracts of dermatophagoides pteronyssinus and dermatophagoides farinae induced strong dth responses and was transferred with t cells. assessment of dth responses to the house dust revealed that substantial cross-reactivity at t-cell level was observed between these two mite extracts. almost all the fractions of the extract of d. pteronyssinus, w ... | 1989 | 2470689 |
| comparative analysis of physicochemical and immunochemical properties of the two major allergens from dermatophagoides pteronyssinus and the corresponding allergens from dermatophagoides farinae. | two major allergens, dp1 (der p i) and dp2 (der p ii), were isolated from the whole culture extract of dermatophagoides pteronyssinus, and the physicochemical and immunochemical properties of these allergens were compared with those of the corresponding allergens from dermatophagoides farinae, df1 (der fi) and df2 (der fii). on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, polyacrylamide gel isoelectric focusing and amino acid analysis, both dp1 and dp2 were demonstrated to have clo ... | 1989 | 2498214 |
| house dust mites in naval ships, military barracks, and homes in the hampton roads area of virginia. | this survey was undertaken to determine the possible exposure of military personnel to house dust mites in ships, barracks, and homes in the hampton roads area of virginia. during the period from april 1, 1982 through august 31, 1983, 524 dust samples were collected with vacuum cleaners. mites were extracted from aliquots of dust, counted, and identified as to species and life stage. dermatophagoides pterynssinus (european house dust mite) was more common in private homes, while dermatophagoides ... | 1989 | 2507967 |
| pyroglyphid mites in the high tatras sanatoria. | 1. in our work we studied the problematics of house dust fauna in the sanatoria and institutes of the tatra region, regarding especially the incidence of mites of the family pyroglyphidae that are considered for the major source of house dust allergen. 2. in eleven places of different altitude in this region, there were in 1982 chosen 13 localities--sanatoria and institutes. here, once quarterly, were collected house dust samples from beds and floors. 3. in the locality 1 helios, strbské pleso, ... | 1989 | 2519180 |
| [pathology caused by acari]. | this report concerns the observations the authors have been carrying out for over twelve years in sardinia; they regard the sensitization to house dust mites (dermatophagoides pteronyssinus, dermatophagoides farinae) and to storage mites (acarus siro, glycyphagus domesticus, lepidoglyphus destructor, tyrophagus putrescentiae) and are been obtained with: 1) skin tests with mite extracts; 2) radioallergosorbenttest (rast) for specific ige and igg; 3) mite exposure in homes and mite analysis; 4) hy ... | 1989 | 2519754 |
| evidence of paf-acether metabolic pathway activation in antigen challenge of upper respiratory airways. | lyso-paf-acether and paf-acether (formerly platelet-activating factor) were detected in nasal secretions from patients with hay fever who underwent local antigen challenge. lyso-paf release was observed in 12 of 13 patients, with a maximum (p less than 0.001) 5 min after stimulation and a progressive decrease during the first hour. paf was detected in the 5-min postchallenge nasal washings from two of 13 subjects. after hplc, this mediator was found in four of seven postchallenge nasal washings ... | 1989 | 2546468 |
| measurement of allergens associated with dust mite allergy. i. development of sensitive radioimmunoassays for the two groups of dermatophagoides mite allergens, der i and der ii. | sensitive radioimmunoassays were developed to measure the two groups of major allergens (der i and der ii) of dermatophagoides pteronyssinus and dermatophagoides farinae, in which radiolabeled protein a served as a general tracer. glass rods covalently coupled with f(ab')2 fragments of rabbit antiserum igg were incubated first with mite or house dust extracts, and then with affinity-purified rabbit antibodies. the bound allergen-antibody complex was detected with a 125i-labeled protein a. the tw ... | 1989 | 2583856 |
| [skin reactivity to grass pollen and the mite dermatophagoides pteronyssinus in the prick-test using 2 types of allergen preparations]. | the reliability of prick testing with allergen preparations produced in yugoslavia was assessed by comparison to the internationally accepted ones (pharmacia diagnostica) of 100,000 bu activity. the following allergens were used: the mite dermatophagoides pteronyssinus in 107 subjects, and grass pollens: dactylis glomerata, poa pratensis, phleum pratense and secale cereale in 39 subjects. the clinical significance of the response was assessed in relation to the skin reaction to the negative cont ... | 1989 | 2638564 |
| [relationship between bronchial asthma and mite]. | we studied the relationship between bronchial asthma and mite as an antigen by reviewing previous reports. the incidence of the bronchial asthma has been increasing and it is estimated to be more than 2% of the total population. the main components of the antigen in the house dust (h, d.), which has been considered to be an important antigen of the perennial asthma, are dermatophagoides farinae (d, f.) and dermatophagoides pteronyssinus (d, p.) in h, d. the housing environment in recent years ha ... | 1989 | 2689700 |
| correlations between levels of mite and cat allergens in settled and airborne dust. | thirty homes in rochester, minn., 20 of which housed cats, were studied to compare cat and mite allergen concentrations in airborne and settled dust. with air sentinels in the bedroom and living room for airborne collections, and a sample vac for collections from living room carpet and bedroom mattress, immunochemical quantifications of each were made with various radiometric assays with polyclonal and monoclonal antibodies. the most significant correlations were found between airborne mite in t ... | 1989 | 2708737 |
| skin and serum reactivity to some storage mites in children sensitive to dermatophagoides pteronyssinus. | the incidence of skin test and rast positivity for dermatophagoides farinae and storage mites (glycyphagus privatus, lepidoglyphus destructor, tyrophagus putrescentiae, acarus siro, and gohieria fusca) have been evaluated in children with bronchial asthma and positive prick test to dermatophagoides pteronyssinus. the storage mite with highest incidence of skin and rast positivity was gohieria fusca, which caused a positive response in more than 50% of the children evaluated. this antigen appears ... | 1989 | 2742217 |
| immunogenicity of monomeric and polymeric house dust mite dermatophagoides pteronyssinus. | a methodology for the preparation of a polymerized antigen from house dust mites dermatophagoides pteronyssinus is described. polymerization was carried out by glutaraldehyde treatment in pbs and sephacryl s300 column fractionation. a polymer of 135 kd was obtained. rabbits immunized with the soluble polymer (hdmp) elaborated specific antibodies as measured by a dot-immunobinding assay. the reaction was inhibited by previous incubation with the hdmp antigen. it is suggested that the polymerized ... | 1989 | 2750636 |
| hypersensitivity to pets in italy. | eight hundred and two consecutive symptomatic outpatients (with asthma and rhinitis) were studied to evaluate the incidence of prick test positivity to cat and dog danders and the association of exposure to these animals in the home, as revealed by a standard questionnaire. 14.3% were found to be skin positive to cat and/or dog danders. the incidence of cutaneous hypersensitivity to cat and/or dog danders was 25% in the 216 subjects who kept animals, significantly higher (p less than 0.0005) tha ... | 1989 | 2787593 |
| the pharmacology of leukotrienes in human airways: in vitro and in vivo studies. | immunological challenge of human lung parenchyma causes formation of arachidonate metabolites: prostaglandin d2 (pgd2) (70% of the formed mediators), leukotrienes e4 (lte4) (15%) and d4 (ltd4) (10%). leukotriene b4 (ltb4) was barely detectable (2%). inhibition of pgd2 formation by indomethacin (15 microm) was approximately 90%, but was not accompanied by redistribution of arachidonate metabolism towards sulphidopeptide leukotrienes, as postulated for aspirin-sensitive asthma. specific binding si ... | 1989 | 2803405 |
| the study of house dust mites in taiwan. | by using a radioallergosorbent test (rast) and intradermal skin testing, our previous studies strongly suggested that house dust mites are the most important allergen in taiwan, especially in asthmatic children. this study was conducted to determine the distribution of house dust mites in houses of mite-sensitive asthmatic children and normals, the species of mites in various house dust samples, the seasonal prevalence, and the relation between the content of mite allergen and the mite density i ... | 1989 | 2919796 |
| clinical significance and allergenic cross-reactivity of euroglyphus maynei and other nonpyroglyphid and pyroglyphid mites. | the clinical significance and allergenic cross-reactivity of the storage mites lepidoglyphus destructor and acarus siro and the house dust mites dermatophagoides pteronyssinus and euroglyphus maynei were investigated with specific ige antibodies by use of the rast and the rast-inhibition technique. rast-positive sera were obtained from 64 swedish farmers whose test results were positive to at least one of the four mite species. e. maynei was shown to be a common cause of sensitization among the ... | 1989 | 2926080 |
| allergenic and antigenic relationship between three species of storage mite and the house dust mite, dermatophagoides pteronyssinus. | we have explored the antigenic and allergenic relationship between the house dust mite dermatophagoides pteronyssinus and three species of storage mite, glycyphagus destructor, acarus siro, and tyrophagus longior. crossed immunoelectrophoresis demonstrated that all the mite extracts contained multiple antigens but that there was only limited cross-reactivity between the different species. six sera were obtained from workers exposed to storage mites and with occupationally related lower respirato ... | 1989 | 2754141 |
| [specific immunotherapy using the slow alpha fraction of dermatophagoides pteronyssinus. efficacy; tolerance]. | this open study was made on 126 patients (72 men, 54 women). specific immunotherapy (it) commenced in 1988 with the dermatophagoides pteronyssinus alpha fraction (alpha -f dpt) a new standardised, purified allergen, of better quality, but more reactive. the patient were aged between 4 and 66 years (mean 23.1 years) the subjects were young and the majority were male. most (93.6%) showed at least respiratory symptoms (asthma, rhinitis). in about a quarter of the patients there were also dermatolog ... | 1989 | 2624679 |
| the inhibitory actions of azelastine hydrochloride on the early and late bronchoconstrictor responses to inhaled allergen in atopic asthma. | we have examined the effect of azelastine hydrochloride, 8.8 mg, on the early and late responses to inhaled allergen in a group of 12 atopic subjects with asthma. on two separate days, 3 weeks apart, patients were administered either oral azelastine, 8.8 mg, or matched placebo. four hours later they inhaled via nebulizer, a dose of allergen (grass pollen or dermatophagoides pteronyssinus) that had previously been demonstrated to produce a 25% fall in fev1. plasma-histamine concentrations and fev ... | 1989 | 2572616 |
| high mite-allergen exposure increases the risk of sensitization in atopic children and young adults. | concentrations of major allergens of dermatophagoides pteronyssinus (dpt) and d. farinae (df), der p i and der f i, were determined in 183 dust samples of mattresses of 133 atopic and 50 nonatopic children by a sandwich-type elisa. atopic children and young adults living in houses with high levels of der p i and der f i (greater than or equal to 2000 ng/gm of dust) were found to have significantly higher serum ige levels to dpt and df (p less than 0.0001) compared to patients with low mite-aller ... | 1989 | 2478606 |
| allergic respiratory disease in grain workers: the role of storage mites. | one hundred thirty-three grain-store workers employed at a regional grain store in essex, u.k., participated in a survey of respiratory symptoms, lung function, bronchial responsiveness to methacholine and skin tests, and specific ige to occupational allergens, including extracts of five storage-mite (sm) species and grain extracts. previously reported associations between occupational respiratory symptoms and cigarette smoking, and symptoms of bronchial hyperresponsiveness to methacholine were ... | 1989 | 2778235 |
| effect of cytokines on spontaneous and allergen-induced cd23 expression, scd23 release and ig(e,g) synthesis from peripheral blood lymphocytes. | we studied the expression of the cd23 antigen as well as the release of soluble cd23 from peripheral blood lymphocytes (pbl) of atopic donors. pbl were stimulated with allergen (dermatophagoides pteronyssinus; der.p.), cytokines (interleukin-4, interferon-gamma; il-4, ifn-gamma) or combinations of stimuli. cd23 levels were enhanced after the addition of allergen or il-4 (10 u/ml) up to 10-fold compared to spontaneous expression. soluble cd23 (scd23) release was enhanced in a dose-dependent manne ... | 1989 | 2530155 |
| house dust mite, dermatophagoides pteronyssinus, and its allergens: effects of washing. | the study was undertaken to evaluate the effect washing at various temperatures would have on the house dust mite, dermatophagoides pteronyssinus, and its allergens. young and old mite cultures underwent a simulated washing programme. to record the number of live mites prior to and after washing a new counting method was applied. the findings demonstrated that although washing reduced the number of live mites, a washing programme at minimum temperature 58 degrees c was required in order to achie ... | 1989 | 2802113 |
| skin-prick test preparations of dermatophagoides pteronyssinus for prediction of a positive response to provocation testing. | skin-prick testing (spt) with allergen is widely used in the investigation of respiratory disease, yet its relationship to the results of provocation testing of the nose or bronchus remains obscure. we have studied this relationship by determining the concentration of dermatophagoides pteronyssinus extract and the associated weal size which resulted in the lowest 'false positive' (fp) and highest 'true positive' (tp) rates when compared to the results of bronchial and nasal provocation testing. ... | 1989 | 2758360 |
| suppression of the late asthmatic reaction by hyposensitization in asthmatic children allergic to house dust mite (dermatophagoides pteronyssinus). | in this study, we wanted to test the hypothesis that hyposensitization (hs) abrogates the late asthmatic reaction (lar). we therefore selected 15 asthmatic children (subjects) sensitized to house dust mite (hdm), as proven by positive prick tests and/or specific ige via the rast. all children demonstrated a positive bronchial provocation test (bpt) to hdm; 14 showed a dual asthmatic reaction and one child showed an isolated lar. all children were treated with anti-asthmatic drugs and received hs ... | 1989 | 2758353 |
| immunologic and clinical evaluation of a 12-month course of specific immunotherapy. | allergen specific immunoglobulins (ige, igg, igg1, and igg4) were evaluated during the course of a 12-month specific immunotherapy (its) in 27 allergic subjects: 12 dermatophagoides pteronyssinus (dp)-, 9 parietaria officinalis (w19)-, six perennial and timothy rye grass (g5 and g6)-sensitive cases. further, the modifications of the specific ig levels were compared with the significant improvement in symptoms and drug consumption observed after 12 months of its. ige levels significantly decrease ... | 1989 | 2742215 |
| enhanced immunodetection of blotted house dust mite protein allergens on nitrocellulose following blocking with tween 20. | the effect of blocking nitrocellulose membranes with the nonionic detergent tween 20 on the detection, by protein blotting, of ige-binding to house dust mite dermatophagoides pteronyssinus allergens has been investigated. tween blocking led to enhanced immunodetection of allergens despite removal of proteins from the membrane when compared to protein blocking agents which did not displace transferred components. the enhancement varied with the different mite components and, for one in particular ... | 1989 | 2743967 |
| immunotherapy with a standardized dermatophagoides pteronyssinus extract. systemic reactions during the rush protocol in patients suffering from asthma. | specific immunotherapy can induce severe systemic reactions (srs), especially in patients with asthma. it also appears that potent standardized extracts may be more often involved in the generation of srs than other extracts. since these srs may be potentially life threatening, it is desirable to predict their onset. a prospective study was carried out in 125 mite-allergic subjects with asthma ranging in age from 4 to 57 years to assess the incidence of srs during a rush immunotherapy (rit) prot ... | 1989 | 2708740 |
| a double-blind study of hyposensitization with an alginate conjugated extract of d. pteronyssinus (conjuvac) in patients with perennial rhinitis. 1. clinical aspects. | the efficacy of hyposensitization with a standardised extract of dermatophagoides pteronyssinus (d. pteronyssinus) conjugated to alginate and containing known amounts of antigen p1 (conjuvac) was tested in a double blind, placebo controlled, multi-centre study in 66 adult patients with perennial rhinitis. patients received 11 weekly injections of increasing concentrations of conjuvac containing from 56 x 10(1) to 448 10(3) iu d. pteronyssinus or placebo injections of the alginate diluent to some ... | 1989 | 2655491 |
| structural studies on the allergen der p1 from the house dust mite dermatophagoides pteronyssinus: similarity with cysteine proteinases. | a major allergen (der p1) has been purified to homogeneity from faecal particles from the house dust mite dermatophagoides pteronyssinus. reversed-phase microbore hplc was employed to fractionate and purify a number of tryptic peptides generated from approximately 20 nmol of purified der p1. n-terminal amino acid sequence analyses were performed on the intact polypeptide and six tryptic peptides yielding 89 unique assignments; this corresponds to 40% of the molecule. these data are compared with ... | 1989 | 2911558 |
| effect of reduction and heat on the detection of house dust mite (dermatophagoides pteronyssinus) allergen der p i by protein blotting. | employment of reducing conditions during sample preparation alters the mobility of the house dust mite (dermatophagoides pteronyssinus) allergen der p i as determined by sodium dodecylsulphate-poly-acrylamide gel electrophoresis (sds-page) from an apparent mw of approximately 25 kd (unreduced) to an apparent mw of approximately 30 kd. probing of nitrocellulose transfers with sera from subjects allergic to d. pteronyssinus showed that reduction of der p i was accompanied by a substantial loss of ... | 1989 | 2759721 |
| isolation and characterization of an allergen-rich fraction derived from cultures of dermatophagoides pteronyssinus. preliminary purification of a protein. | an allergen-rich fraction derived from mature cultures of dermatophagoides pteronyssinus was isolated. results of radio-allergosorbent tests and the disclosure of ige-binding proteins after their electrophoretic transfer from sodium dodecyl sulphate-polyacrylamide gel to nitrocellulose sheets revealed that both important groups of allergens, der p i and der p ii, seem to be contained in the above-mentioned fraction. analysis using crossed immuno-electrophoresis (cie) disclosed 5-7 antigens and a ... | 1989 | 2731992 |
| investigation of the allergenic activity of isolated fractions of a standardized partly purified whole mite body (dermatophagoides pteronyssinus) extract. | twelve fractions of a molecular weight range of 1.35-670.00 kilodaltons (kd) were isolated from a biologically standardized partly purified whole mite body extract (dermatophagoides pteronyssinus) by preparative size exclusion high-performance liquid chromatography. the allergenic activity and the antigen and allergen patterns of the isolated fractions were investigated by rast, rast inhibition and crossed (radio)immunoelectrophoresis (cie/crie). by crie, each of the fractions showed allergen pa ... | 1989 | 2592112 |
| guanine content and dermatophagoides pteronyssinus allergens in house dust samples. | the study of the relationship between the guanine content and dermatophagoides pteronyssinus (dp) allergens in house dust samples is reported. mattress and carpet dust of bedrooms from 22 different homes constituted the house dust samples. the guanine content was determined by quantitative measurements and the mite allergenicity by two immunochemical assays with a partially purified extract of dp as internal reference: rast inhibition and crossed and rocket line immunoelectrophoresis. a large sc ... | 1989 | 2715551 |
| [the determination of allergen-specific ige and igg antibodies in patients sensitized to the house dust mite dermatophagoides pteronyssinus]. | 45 patients, hypersensitive to house-dust mites, were examined by the method of skin tests to d. pteronyssinus allergen. besides, in their blood sera the levels of allergen-specific ige antibodies were determined in the radioallergosorbent test and allergen-specific igg antibodies, in the enzyme immunoassay. these tests revealed that in 91% of the patients the results of skin tests were positive, in 68% an elevated level of specific ige antibodies and in 93% of the patients an elevated level of ... | 1990 | 2099077 |
| development of dermatophagoides pteronyssinus (acari: pyroglyphidae). | developmental times (egg to adult) and fecundity of the european house dust mite, dermatophagoides pteronyssinus (trouessart), were determined for various temperatures that normally occur in the mite microhabitat in homes. egg-to-adult developmental times were 122.8 +/- 14.5, 34.0 +/- 5.9, 19.3 +/- 2.5, and 15.0 +/- 2.0 d (means +/- sd) at 16 (61 degrees f), 23 (73 degrees f), 30 (86 degrees f), and 35 degrees c (95 degrees f), respectively. developmental times of individual life stages were con ... | 1990 | 2280388 |
| [result of "as is patch test" using mite elements in atopic dermatitis (ad) patient. 2nd report, "mite lipid"]. | the antigenicity of mite lipid was studied, taking atopic dermatitis as an allergic disease. lipid was extracted from dermatophagoides pteronyssinus (dp) and d. farinae (df). it was tentatively designated a mite lipid antigen (dp-l, df-l), and then a patch test was performed. an extract from animal food (lc) and acetone were used as the controls. with only dp-l, a positive reaction of more than one plus (+) of the icdrg standard was noted in 3 out of 21 cases of ad. histology of the above-mentio ... | 1990 | 2273576 |
| [effect of specific desensitization therapy in combination with an immunopotentiator, neurotropin, on perennial nasal allergy--with special reference to mite specific igg and igg4 antibody levels]. | we treated perennial nasal allergy with specific desensitization in combination with or without neurotropin injections (nsp) from march 1983 to september 1984, and analyzed blood anti-mite igg and igg4 levels in relation to therapeutic efficacy. desensitization and nsp were given once a week and seventy subjects (33 from the desensitization group and 37 from the desensitization + nsp group) were evaluated at 24, 36 and 48 weeks. 1) in total cases clinical usefulness showed no significant differe ... | 1990 | 2260931 |
| a double-blind study of hyposensitization with an alginate-conjugated extract of dermatophagoides pteronyssinus (conjuvac) in patients with perennial rhinitis. ii. immunological aspects. | in a 2-year double-blind placebo controlled study an immunological evaluation was carried out on 33 patients (15 males, 18 females, mean age 29.2 years) with mite-induced perennial rhinitis who were submitted to specific immunotherapy (it) with an alginate-conjugated extract of d. pteronyssinus. the behaviour of ige, igg, igg1 and igg4 antibodies specific to d. pteronyssinus and its major allergen der p1 was characterized by assessment of their changes in serum, and changes in igg in nasal secre ... | 1990 | 2252161 |
| a placebo-controlled trial of immunotherapy with two extracts of dermatophagoides pteronyssinus in allergic rhinitis, comparing clinical outcome with changes in antigen-specific ige, igg, and igg subclasses. | a double-blind, placebo-controlled trial of immunotherapy was conducted in patients with dermatophagoides pteronyssinus rhinitis. thirty patients received an extract with a high content of der p i (pharmalgen), 20 received a conventional mite extract (allpyral), and 30 patients received histamine chloride (placebo). specific igg and subclasses were measured before and after 3 and 12 months of treatment by ria and/or elisa, and specific ige by rast. clinical outcome was assessed by skin prick tes ... | 1990 | 1699985 |
| evolution of the late asthmatic reaction during immunotherapy and after stopping immunotherapy. | in previous studies it was demonstrated that the frequency and the severity of the late asthmatic reaction (lar) can be attenuated by immunotherapy (it). the present study was set up to observe the evolution of the lar under it and after having stopped it. nineteen children with bronchial asthma and an lar to house dust mite (dermatophagoides pteronyssinus) (hdm) were selected for this study. all subjects received it with hdm extract during 1 year. thereafter, the children were divided randomly ... | 1990 | 2384645 |
| temporal association between der pi exposure, immediate hypersensitivity and clinical severity of eczema. | uncontrolled studies of avoidance of house dust mite (dermatophagoides pteronyssinus) by atopic subjects have reported clinical improvement in the severity of eczema. the aim of the present study was to examine the temporal relationship between environmental exposure to the major mite allergen (der pi) and clinical disease severity in children with chronic atopic eczema. twelve children were identified as being house dust mite-sensitive on the basis of skin prick test and rast to der pi. they we ... | 1990 | 2244674 |
| effect of heat treatments on the house-dust mites dermatophagoides pteronyssinus and d. farinae (acari: pyroglyphidae) in a mattress-like polyurethane foam block. | the behaviour and survival of the house-dust mites dermatophagoides pteronyssinus and d. farinae were studied under laboratory conditions in a mattress-like polyurethane foam block that was heated by an electric blanket. mites obtained from a laboratory culture were introduced into the foam block which was sliced into seven horizontal layers and covered with an electric blanket. measurements of temperature and relative humidity were taken between the layers and the number of living mites was est ... | 1990 | 2226071 |
| comparison of diversity and function of house dust mite-specific t lymphocyte clones from atopic and non-atopic donors. | panels of cd4+cd8- t lymphocyte clones (tlc), specific for house dust mite dermatophagoides pteronyssinus (dp) proteins, were generated from the peripheral blood of an atopic dp-allergic donor (ad), suffering from severe atopic dermatitis, and a histocompatible non-atopic donor (nad). we studied the diversity of tlc within these two panels in search for the possible occurrence of dominant clone types with properties that might be characteristic for the atopic or non-atopic state. tlc with specif ... | 1990 | 2387315 |
| house dust mite sensitivity: a comparison of immune response with exposure to four species of pyroglyphidae. | twenty-five atopic children under 11 years of age were studied, using skin and rast tests, for their specific ige response to four species of pyroglyphid house dust mites, dermatophagoides pteronyssinus, d. farinae, d. microceras and euroglyphus maynei. all of the children were sensitive to d. pteronyssinus, 20 (80%) of these children were also sensitive to d. farinae and d. microceras, and 16 of the latter (64%) were also sensitive to e. maynei. dust samples from various sites in the homes of t ... | 1990 | 2364312 |
| late asthmatic reaction to perennial and seasonal allergens. | twelve patients with asthma sensitized to dermatophagoides pteronyssinus and parietaria judaica were studied. patients were challenged before (winter), during (summer), and after (autumn) parietaria-pollen season. early asthmatic reaction was positive for both allergens, and the provocative dose causing a 15% decrease of fev1 was similar in all seasons. the provocative dose of methacholine causing a 15% decrease of fev1 and specific iges for both allergens were also not significantly different t ... | 1990 | 1692048 |
| who will benefit from hyposensitization? predictive parameters in house dust mite allergic asthmatics. | the aim of this study was to assess the ability of various data collected before treatment to predict the therapeutic benefits of hyposensitization. thirty-one asthmatics were hyposensitized with extract from the house dust mite dermatophagoides pteronyssinus (dp) for 2 years, 15 comparable patients served as controls. the treatment extract was either modified by coupling to monomethoxypolyethylene glycol (mpeg) or administered in a diluent containing al(oh)3. improvement would be either a great ... | 1990 | 2327561 |
| the effect of inhaled fenoterol, administered during the late asthmatic reaction to house dust mite (dermatophagoides pteronyssinus). | a double-blind, placebo-controlled crossover study was set up to investigate the effect of fenoterol (400 micrograms) during the late asthmatic reaction (lar). twenty young subjects with asthma (mean age, 11.8 years; range, 8.3 to 20.6 years) were selected on the basis that they developed an lar after bronchial challenge with dermatophagoides pteronyssinus. after the lar occurred, a placebo and fenoterol were administered blindly by a metered-dose inhaler, with an interval of 15 minutes and in a ... | 1990 | 2324409 |
| allergic bronchial asthma due to dermatophagoides pteronyssinus hypersensitivity can be efficiently treated by inoculation of allergen-antibody complexes. | antigen-antibody complexes were made from allergens of the common house dust mite, dermatophagoides pteronyssinus (dpt) and an excess of purified autologous specific antibodies. these complexes have been used to treat dpt-hypersensitive patients who suffered from chronic bronchial asthma. clinical symptoms and medication intake were followed by filling in diary cards. peak expiratory flow, measured four times a day, was also followed. intradermal skin tests and bronchial challenge tests were per ... | 1990 | 2318962 |
| ecology of house dust mites in oxfordshire. | in a survey of the mite fauna in 30 homes in oxfordshire, dermatophagoides pteronyssinus and euroglyphus maynei were found to be the most abundant species but d. farinae was absent. of a total of 14 parameters investigated for their influence on mite numbers, relative humidity was apparently the most important factor. bedrooms with humidities above 64% contained significantly more mites in mattresses than those with lower humidities. bedroom humidity and thus mattress mite numbers were apparentl ... | 1990 | 2357619 |
| the use of phadiatop in mass-screening programmes of inhalant allergies: advantages and limitations. | the validity of phadiatop as a tool in the mass screening for inhalant allergies was investigated. seventy-nine out of 600 recruits (13.2%) were classified as allergic to inhalant allergens (68 oculorhinitis; 11 asthma) on the basis of positive history, confirmed by skin testing and/or rast for the seven most common aero-allergens in italy. another 74 subjects had positive rast and/or skin tests, yet had never experienced allergic symptoms. phadiatop was positive in 145/600: in 78 out of 79 alle ... | 1990 | 2357615 |
| [accelerated desensitization with dermatophagoides pteronyssinus in severe asthmatic children. evaluation after one year of immunotherapy]. | an homogeneous group of 17 children with severe chronic asthma were given a rush immunotherapy with standardized dermatophagoides pteronyssinus extracts. maintenance dose was injected monthly for one year. symptom and medication scores as well as functional (lung function tests, specific nasal challenges), immunologic and allergic parameters (skin tests, total serum ige, specific ige and igg4) were recorded before and at the end of the study. rush immunotherapy was well tolerated in spite of som ... | 1990 | 2111693 |
| immunotherapy with a standardized dermatophagoides pteronyssinus extract. iv. systemic reactions according to the immunotherapy schedule. | specific immunotherapy with standardized extracts can induce systemic reactions (srs), possibly increased by a rush immunotherapy (rit) protocol. a prospective study in 1152 mite-allergic patients (3 to 63 years of age) examined the incidence of srs during an rit or a step protocol. all patients received the same standardized extract of dermatophagoides pteronyssinus with the same maintenance dose. in the first group, 290 patients had an rit protocol without any preventive measure. in a second g ... | 1990 | 2406324 |
| hyposensitization in asthmatics with mpeg-modified and unmodified house dust mite extract. iv. occurrence and prediction of side effects. | a double-blind study on hyposensitization (hs) with two extracts prepared from the house dust mite dermatophagoides pteronyssinus (dp) was performed on a group of asthmatics with bronchial sensitivity to dp. in 18 patients, aluminium-hydroxide was added to the dp-extract to give a depot effect (dp-group). nineteen patients were treated with a similar extract in which allergenicity had been reduced by coupling to monomethoxypolyethylene glycol (mpeg-dp-group). this extract had previously been sho ... | 1990 | 2316824 |
| [the fauna, number and spatial distribution of mites (acari) in house dust in moscow]. | there is a wide and strong allergenic background in moscow, which is determined by relatively high population density and regular frequency of the allergenic mites in various premises. 34 species have been identified, 5 of them are allergen producers. the mites of the family pyroglyphidae are the main component of the house dust fauna. dermatophagoides pteronyssinus and d. farinae distributed all over the world are predominate. a comparative evaluation of the fauna structure, numbers and frequen ... | 1990 | 2346769 |
| the spectrum of low molecular weight house dust mite (dermatophagoides pteronyssinus) allergens with emphasis on der p ii. | analysis by protein blotting of sera from 96 different house dust mite-allergic subjects revealed previously unrecognized complexity of low molecular weight (mw) (less than 20 kd) ige-binding proteins in extracts of whole bodies of dermatophagoides pteronyssinus. of 11 different ige-binding components of mw less than 20 kd identified, two (mw approximately 16 kd and approximately 15 kd), showed both a high frequency (88% and 49% respectively) and a high intensity of ige-binding. the approximatel ... | 1990 | 2310979 |
| [results of skin tests 1981 to 1989]. | in the aim of proving the existence of organism sensitivity to standard group inhalation antigens, at the orl clinic in novi sad, in the period of 1981 through july, 1989, intradermal testing was conducted in 1816 patients. the greatest number of positive reactions appeared within the city inhabitants, mostly of female sex. most frequently they reacted to house dust (53.19%), pollen (37.88%), ambrosia (18.17%), dermatophagoides pteronissynus (11.17%), and much less frequently to other allergens. ... | 1990 | 2077378 |
| [allergic individuals to japanese cedar or orchard grass consist of two subgroups based on the sensitization to dermatophagoides pteronyssinus]. | one hundred and twenty-six allergic subjects were divided into three groups based on the rast results for dermatophagoides pteronyssinus (d.p) and japanese cedar (j.c.). we compared sex, age of examination for rast, serum ige, family history of allergic diseases, prevalence rate of bronchial asthma (ba) and allergic rhinitis (ar), and onset of ar among three groups. no significant difference in the other variables except serum ige and prevalence rate of ba was found between the group with d.p-po ... | 1990 | 2222193 |
| evidence for compartmentalization of functional subsets of cd2+ t lymphocytes in atopic patients. | lymphokine secretion profiles were studied of human allergen-specific cd4+ t lymphocyte clones (tlc). to this aim, panels of house dust mite dermatophagoides pteronyssinus (dp)-specific tlc were generated from two atopic dp-allergic patients, suffering from severe atopic dermatitis (ad1) and allergic asthma (ad2), respectively, and from a non-atopic individual (nad). from ad1 additional tlc were cloned specific for tetanus toxoid or candida albicans, both ag that were not relevant for the atopic ... | 1990 | 1972164 |