Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the carbohydrate moieties of suppressor igg-binding factor released by murine t cells. | the carbohydrate moieties of murine igg-binding factor (igg-bf) were studied using lectins binding n-glycosylated sequences such as concanavalin a (con a), lens culinaris agglutinin (lca), and wheat germ agglutinin (wga), and lectins binding o-glycosylated sequences such as peanut agglutinin (pna) and helix pomatia agglutinin (hpa). sources of igg-bf were: (1) supernatants from t2d4, a t cell hybridoma constitutively producing igg-bf, and (2) factor purified by affinity chromatography on rabbit ... | 1987 | 3500399 |
| ultrastructural localization of helix pomatia lectin-binding sites in mouse lung elastic fibers. | helix pomatia (snail) lectin complexed with colloidal gold (hpl-gold) recognized binding sites on elastic fibers in plastic embedded sections of lung tissue from mice of several ages. deposition of the lectin-gold particles was examined by electron microscopy. structures such as the elastic laminae of pulmonary vessels and elastic fibers throughout the lung was specifically and intensely decorated by the hpl-gold complex and easily visualized. the binding of the hpl-gold particles was primarily ... | 1987 | 3436842 |
| glycoconjugates of the human sperm surface: distribution and alterations that accompany capacitation in vitro. | we have studied changes in the binding of fluoresceinated lectins to human sperm during in vitro capacitation. we first determined the surface labeling pattern of viable sperm obtained by the swim-up procedure. sperm were labeled with 100 micrograms/ml fitc-conjugated lectin at 4 degrees c for 30 min. we simultaneously used hoechst stain 33258 as a supravital stain to help differentiate surface from intracellular lectin labeling. of 14 lectins studied, six (phytohemagglutinin-e, concanavalin a, ... | 1987 | 3147937 |
| the reaction of nitric oxide with copper proteins and the photodissociation of copper-no complexes. | the reactivity with nitric oxide was investigated for a number of type-1, type-2 and type-3 copper proteins azurin from pseudomonas aeruginosa (type-1 copper); bovine superoxide dismutase, diamine oxidase from pig kidney and galactose oxidase from dactylium dendroides (type-2 copper); haemocyanin from helix pomatia (type-3 copper); the blue oxidases ceruloplasmin from pig serum, and ascorbate oxidase from cucurbita pepo medullosa. type-1 copper formed complexes with no in the oxidised state, whi ... | 1987 | 2822126 |
| cytotoxic activity in the coelomic fluid of the annelid eisenia foetida sav. | the annelid eisenia foetida not only causes hemolysis of red blood cells of several vertebrate species, but also has a toxic effect on a variety of cell types, such as chicken fibroblasts, guinea-pig polymorphonuclear leukocytes and insect hemocytes. however, it has no influence on the vitality of the coelomocytes of lumbricus terrestris and other lumbricides, nor on the hemocytes of the snail helix pomatia, the mussels anodonta cygnea and unio tumidus, free cells of the turbellarian euplanaria ... | 1987 | 3571568 |
| helix pomatia and ulex europeus lectin binding in human breast carcinoma. | formalin fixed, paraffin embedded tissue from 100 consecutive cases of breast carcinoma were studied for binding with helix pomatia (hpa) and ulex europeus (uea1) lectins. serial sections were pretreated with trypsin or neuraminidase to determine the effect of these enzymes on lectin binding. the lectins were visualized by the peroxidase antiperoxidase technique and the cell staining proportion assessed in a semi-quantitative manner under the light microscope. correlating staining with prognosti ... | 1987 | 3309230 |
| lectin-binding characteristics of wuchereria bancrofti microfilariae. | the binding of 10 different lectins to the surface of microfilariae of wuchereria bancrofti has been investigated. wheat germ agglutinin (wga) and helix pomatia lectin (hpa) bound specifically to the sheathed microfilariae indicating the presence of n-acetyl-d-glucosamine and n-acetyl-d-galactosamine respectively on the surface. exsheathed microfilariae did not react with any of the lectins. treatment of sheathed microfilariae with proteases resulted in increased binding of wga and hpa. such tre ... | 1987 | 2884837 |
| host cell-induced differences in o-glycosylation of herpes simplex virus gc-1. i. structures of nonsialylated hpa- and pna-binding carbohydrates. | lectins with narrow oligosaccharide specificities were established as probes to study the host cell influence on the biosynthesis of o-linked oligosaccharides of the herpes simplex virus type 1 (hsv-1)-specified glycoprotein c (gc-1). we found that only gc-1 and no other glycoprotein bound to the peanut lectin (pna), with main specificity for gal(beta 1-3)galnac. previously, we have shown that only gc-1 binds to the helix pomatia lectin (hpa), with main specificity for terminal galnac. the o-lin ... | 1987 | 2825412 |
| lectin histochemistry of plaques and tangles in alzheimer's disease. | biotinyl derivatives of several lectins and avidin-horseradish peroxidase were used to study the localization of glycoconjugates in amyloid plaques and in neuritic tangles in brains of patients with alzheimer's disease (ad), downs syndrome (ds) and gerstmann-sträussler syndrome (gss). the lectins tested recognize the following residues: beta-d-galactosyl [ricinus communis agglutinin 120, (rca-1) and peanut agglutinin, (pna)]; alpha-d-galactosyl [griffonia simplicifolia agglutinin (gsa)]; alpha-d ... | 1987 | 2955631 |
| ultrastructural demonstration of maclura pomifera agglutinin binding sites in the membranocystic lesions of membranous lipodystrophy (nasu-hakola disease). | this paper reports three cases of membranous lipodystrophy (nasu-hakola disease) in two families and studies the carbohydrate components of membranocystic lesions in all three cases, using twelve kinds of lectins labelled by horseradish peroxidase (hrp). maclura pomifera agglutinin (mpa), which specifically binds alpha-d-galactose residues, strongly stained typical membranocystic lesions, whereas the other lectins did not. however, helix pomatia agglutinin (hpa), which specifically binds to n-ac ... | 1988 | 3144083 |
| enteric neuromuscular junctions: comparison of ultrastructural features in different phylogenetic groups. | the enteric neuromuscular junctions of snail (helix pomatia), locust (locusta migratoria migratorioides), cockroach (periplaneta americana), carp (cyprinus carpio) and tench (tinca tinca) were studied by means of different light and electron microscopic methods. the nitroblue tetrazolium staining revealed that the myenteric plexuses of the above species are composed of nerve cells, a network of varicose nerves and nerve bundles. instead of highly organized ganglia, single neurons or small groups ... | 1988 | 3393275 |
| the reaction of hydroxyurea with oxyhaemocyanin and methaemocyanin of the crayfish astacus leptodactylus and the snail helix pomatia. | the reaction of hydroxyurea with the oxyhaemocyanins of astacus leptodactylus and helix pomatia yielded methaemocyanins which could be regenerated with hydroxylamine. hydroxyurea did not react with astacus methaemocyanin, but quantitatively regenerated helix methaemocyanin under n2. the reaction of hydroxyurea with helix haemocyanin at ph 5.7 under air thus led to a steady state, with an oxyhaemocyanin/methaemocyanin ratio of 2.05:1. | 1988 | 3178776 |
| detection of b and t cells, with lectins or antibodies, in healthy and bovine leukemia virus-infected cattle. | lectins, polyclonal antibodies and monoclonal antibodies (mabs) were evaluated as markers for bovine lymphocytes obtained from healthy animals and from cattle infected with bovine leukemia virus (blv). in the blood from healthy cattle the proportion of cells identified as t lymphocytes with the lectin helix pomatia (hp) (67.8 +/- 6.2%) using the indirect immunofluorescence technique was similar to the proportion of cells identified by the mabs p5 (66.1 +/- 3.8%) and blt-1 (59.8 +/- 7.1%). the pr ... | 1988 | 2839934 |
| lectin-binding sites in squamous cell carcinomas and keratoacanthomas. | the lectin-binding pattern of malignant and benign human epidermal tumours was studied. twelve squamous cell carcinomas and ten kerato-acanthomas were investigated, using fluorochrome-conjugated lectins. lectins used were canavalia ensiformis agglutinin (con-a), helix pomatia agglutinin (hpa), phaseolus vulgaris agglutinin (pha-l), and ricinus communis agglutinin (rca-120). two types of fluorescence pattern, intercellular 'pemphigus-like' and peritumoral, were noted. both allowed classification ... | 1988 | 2467485 |
| cytochemical characterization of mouse l1210 leukemia. | mouse lymphatic leukemia l1210 cells were characterized with polyclonal and monoclonal antibodies and lectins. the cells were found to have the phenotype igg-, thy-1.2-, lyt-1-, lyt-2-, asialo-gm1-, tl-, i-ad-, il-2r+, peanut agglutinin+, and helix pomatia lectin +/-. they retained expression of h-2kd and h-2dd. thus, these cells resemble "null" cells. | 1988 | 2466781 |
| the subcellular localization of apomucin and nonreducing terminal n-acetylgalactosamine in porcine submaxillary glands. | antibodies prepared against enzymatically deglycosylated porcine submaxillary gland mucin (apomucin), which were unreactive with native mucin and its partially deglycosylated derivatives, were used to immunolocalize apomucin in situ. electron microscopy of sections of lowicryl k4m-embedded tissue reacted successively with antibodies and protein a-gold complexes showed apomucin exclusively in mucous cells within the rough endoplasmic reticulum, transitional elements of the endoplasmic reticulum, ... | 1988 | 3339018 |
| cytochemical localization of blood group substances in human salivary glands using lectin-gold complexes. | we investigated localization of blood group antigens and their related substances in human labial salivary and submandibular glands by application of a post-embedding cytochemical staining procedure using lectin- or glycoprotein-gold complexes. surgical tissue was obtained from 10 patients. blood group-specific lectins, such as dolichos biflorus agglutinin or helix pomatia agglutinin (group a-specific), griffonia simplicifolia agglutinin-i b4 (group b-specific), and ulex europaeus agglutinin i ( ... | 1988 | 3346537 |
| ultrastructural studies of glycoconjugates in brain micro-blood vessels and amyloid plaques of scrapie-infected mice. | lectin or glycoprotein-gold complexes and samples of scrapie-infected mouse brain embedded in lowicryl k4m were used for ultrastructural localization of glycoconjugates. the lectins tested recognize the following residues: beta-d-galactosyl [rca, ricinus communis agglutinin (aggl.) 120], n-acetyl and n-glycolyl neuraminic acid (lfa, limax flavus aggl.), n-acetyl-d-glucosaminyl and sialyl (wga, wheat germ aggl.), n-acetyl-d-galactosaminyl (hpa, helix pomatia aggl., and dba, dolichos biflorus aggl ... | 1988 | 3348084 |
| lectin-gold labeling of glycoconjugates in normal and brattleboro rat papilla: effect of vasopressin. | some reports suggest that the plasma membrane glycocalyx of collecting duct epithelial cells, as well as interstitial glycoconjugates, may be involved in vasopressin action and urinary concentration. in view of this, we have used the lectin-gold technique to map and quantify helix pomatia lectin (hpl)-binding sites in the inner medulla of kidneys from normal long-evans rats, vasopressin-deficient brattleboro rats, and brattleboro rats treated for up to 5 wk with exogenous vasopressin. the result ... | 1988 | 3348385 |
| glycolipid-lectin interactions: reactivity of lectins from helix pomatia, wisteria floribunda, and dolichos biflorus with glycolipids containing n-acetylgalactosamine. | the autoradiographic detection of 125i-labeled lectins binding to glycolipids on thin-layer chromatograms can be used to rapidly analyze total glycolipid extracts of cells or tissues for specific oligosaccharide structures. the helix pomatia lectin which binds with high affinity to terminal alpha-linked galnac residues did not bind to globoside (terminal beta 1-3galnac) but did bind the ganglioside gm2 and its asialo derivative which have terminal beta 1-4galnac residues. the lectin from dolicho ... | 1988 | 3355161 |
| biosynthesis of the human sucrase-isomaltase complex. differential o-glycosylation of the sucrase subunit correlates with its position within the enzyme complex. | the biosynthesis and maturation of human sucrase-isomaltase (si, ec 3.2.1.48-10), was studied in cultured small intestinal biopsy specimens and mucosa explants. pulse-chase experiments with [35s]methionine revealed one high mannose intermediate of mr = 210,000 (pro-sih) which was processed at a slow rate to an endo h-resistant, mature form of mr = 245,000 (pro-sic). the fully core-glycosylated form (mr = 212,000) was detected only when 1-deoxynojirimycin was added to the culture medium, thus ind ... | 1988 | 3366777 |
| purification of forssman and human blood group a glycolipids by affinity chromatography on immobilized helix pomatia lectin. | a method for the affinity purification of intact glycolipids having nonreducing terminal alpha 1-3 linked n-acetylgalatosamine residues has been developed. this technique relies on the retention of the carbohydrate-binding specificity of immobilized helix pomatia lectin in aqueous solutions of tetrahydrofuran. both forssman glycolipid and a mouse blood group a-active hexaosylceramide were bound by columns of the lectin equilibrated in a solvent containing 95% tetrahydrofuran and 5% water. after ... | 1988 | 3389512 |
| structure, biosynthesis, and glycosylation of human small intestinal maltase-glucoamylase. | maltase-glucoamylase (mga) was immunoprecipitated from detergent extracts of brush border membranes of the human small intestinal mucosa. electrophoretic analysis of the precipitates under denaturing conditions revealed a single polypeptide of mr = 335,000 in the presence or absence of reducing agents. cross-linking of brush border membranes with the homobifunctional reagent dithiobis(succinimidylpropionate) did not result in considerable changes in the electrophoretic pattern of mga. in contras ... | 1988 | 3143729 |
| heterogeneity of apical glycoconjugates in kidney collecting ducts: further studies using simultaneous detection of lectin binding sites and immunocytochemical detection of key transport enzymes. | in the search for a functional role for the polarized glycoconjugates of rat collecting duct epithelial cells, the relation between binding of various lectins and expression of cellular transport enzyme profile of the cells was studied. for this purpose, principal and intercalated cells of rat kidney collecting duct were identified by morphological criteria and by their immunocytochemically determined content of (na+ + k+)-atpase and carbonic anhydrase (ca ii), respectively. various n-acetylgala ... | 1988 | 2853698 |
| structure of a ganglioside with cad blood group antigen activity. | the cad antigen is a rare erythrocyte blood group antigen expressed on both sialoglycoprotein and ganglioside structures. it is related both serologically and biochemically to the sda blood group antigen expressed on over 90% of caucasian erythrocytes. we reported previously that cad erythrocytes contain a novel ganglioside that binds helix pomatia lectin and inhibits human anti-sda antibody. we have now purified the cad ganglioside and determined its structure. the ganglioside contained glc-gal ... | 1988 | 3167001 |
| wheat germ agglutinin and helix pomatia agglutinin lectin binding on cochlear hair cells. | the fluorescein labelled lectins fitc-wga and fitc-hpa were used to identify specific carbohydrates in cochlear hair cells. wheat germ agglutinin (wga) bound with the cell coat of both inner and outer hair cells (ihc and ohc) suggesting the presence of either n-acetyl-d-glucosamine or sialic acid. in contrast, glycoconjugates with terminal n-acetyl-d-galactosamine residues that bind with helix pomatia agglutinin (hpa), were demonstrated inside the plasma membrane of outer hair cells. wga and hpa ... | 1988 | 3170357 |
| backscattered electron imaging of lectin binding sites in tissues following freeze-fracture cytochemistry. | recently, cytochemical techniques have been applied for localizing membrane components; however, transmission electron microscopy only provides two-dimensional information about their distribution. scanning electron microscopy, on the other hand, offers the possibility of examining the three-dimensional architecture of biological samples. the fracture-label cytochemical technique was combined with the backscattered electron imaging (bei) mode of the scanning electron microscope to visualize the ... | 1988 | 3199220 |
| immunoglobulin genes, hla-b8/dr3, and immune responsiveness to primary immunogen and mitogens in normal subjects. | the genetic regulation of immune responsiveness by genes from two independent, highly polymorphic genetic systems, namely immunoglobulin allotypes and human leukocyte antigens (hla), was studied in 35 healthy caucasian volunteers. the in vivo igg class antibody response to the primary test immunogen alpha-helix pomatia hemocyanin (hph) was increased in subjects with the gm1,17;..;21 haplotype compared to that of the non-gm1,17;..;21 group. the igm-class response tended to be higher in the former ... | 1988 | 3259481 |
| inhibition of phosphatase and sulfatase by transition-state analogues. | the inhibition constants for vanadate, chromate, molybdate, and tungstate have been determined with escherichia coli alkaline phosphatase, potato acid phosphatase, and helix pomatia aryl sulfatase. vanadate was a potent inhibitor of all three enzymes. inhibition of both phosphatases followed the order wo4(2-) greater than moo4(2-) greater than cro4(2-). the ki values for potato acid phosphatase were about 3 orders of magnitude lower than those for alkaline phosphatase. aryl sulfatase followed th ... | 1988 | 3280015 |
| alpha-n-acetylgalactosaminidase deficiency, a new lysosomal storage disorder. | a new lysosomal storage disease with autosomal recessive inheritance is described in two male siblings of 5 1/2 and 4 years of age. clinical manifestations started after 9 months of age with neurological symptoms, followed by progressive psychomotor deterioration. urinary oligosaccharide excretion was abnormal and showed a characteristic pattern on chromatography. enzyme assays showed a profound deficiency of lysosomal alpha-n-acetylgalactosaminidase in cultured fibroblasts, leukocytes and plasm ... | 1988 | 3149698 |
| detection of ocular mucus in normal human conjunctiva and conjunctiva from patients with cicatricial pemphigoid using lectin probes and histochemical techniques. | conjunctival biopsies from patients with cicatricial pemphigoid affecting the conjunctiva and patients undergoing cataract surgery (normal conjunctiva) were snap-frozen, cryostat sectioned and incubated with fluorescein-conjugated lectins; peanut agglutinin (pna), helix pomatia agglutinin (hpa), soybean agglutinin (sba), wheat germ agglutinin (wga) and succinylated wheat germ agglutinin (s-wga). controls consisted of preincubating the lectins with the appropriate blocking sugars before applying ... | 1988 | 3289955 |
| distribution of lectin-binding sites in oxyntic and chief cells of isolated rabbit gastric glands. | the distribution of lectin-binding sites in oxyntic and chief cells of isolated rabbit gastric glands was determined with seven fluoresceinated lectins, to ascertain which lectins might best be used in the biochemical characterization of cell membranes and glycoproteins of these two cell types. oxyntic cell canaliculi were labeled by wheat germ, helix pomatia, and peanut lectins, suggesting a predominance of n-acetylhexosamines. tubulovesicles were heavily stained by wheat germ, helix pomatia, a ... | 1988 | 3134267 |
| dolichos biflorus agglutinin (dba) reacts selectively with mast cells in human connective tissues. | dolichos biflorus agglutinin (dba) binds to n-acetyl-d-galactosamine (galnac) residues in glycoconjugates and agglutinates erythrocytes carrying blood group antigen a. in cryostat sections of various tissues from blood group-specified humans, fluorochrome-coupled dba bound preferentially to fusiform connective tissue cells and to certain epithelial cells. the connective tissue cells were identified as mast cells by their typical metachromasia in consecutive staining with toluidine blue. double l ... | 1988 | 3138306 |
| surface carbohydrate changes on onchocerca lienalis larvae as they develop from microfilariae to the infective third-stage in simulium ornatum. | use was made of seven fitc labelled lectins as tools to investigate the surface of onchocerca lienalis larvae as they develop through to the infective third-stage in a natural vector, simulium ornatum. the lectins were derived from canavalia ensiformis (con a), lens culinaris (lentil), triticum vulgaris (wheat germ), arachis hypogaea (peanut), helix pomatia, phaseolus vulgaris (kidney bean) and tetragonolobus purpureus (asparagus pea). between 70 and 100 living parasites were examined for each d ... | 1988 | 3142948 |
| alterations in glycoconjugate expression following malignant transformation of human oropharyngeal squamous mucosa. | malignant transformation of the squamous mucosa in the human oropharynx may be accompanied by alterations in carbohydrate production. glycoconjugate expression was assessed by examining lectin-binding patterns and abh isoantigen expression in human biopsy specimens from nine cases of carcinoma in situ and ten cases of invasive squamous cell carcinoma as well as adjacent normal squamous mucosa. ten different fluorescein-conjugated lectins were used. each lectin binds to specific nonreducing end-t ... | 1989 | 2913429 |
| differential expression of galactose and n-acetylgalactosamine residues during fetal development and postnatal maturation of rat glomeruli as revealed with lectin conjugates. | a battery of fluorochrome- or peroxidase-coupled lectins, reacting with alpha- or beta-galactose (gal), terminal n-acetylgalactosamine (galnac), or gal-(beta 1-3)-galnac residues, was used to study the emergence and distribution of cellular glycoconjugates in developing and adult rat glomeruli. neuraminidase pretreatment of the specimens was applied to monitor the maturation of the glomerular sialoglycoprotein coat. in the adult glomeruli, the lectin conjugates applied reacted sparsely or not at ... | 1989 | 2923282 |
| a new method for hydrolyzing sulfate and glucuronyl conjugates of steroids. | a new method for hydrolyzing steroid conjugates (both sulfates and glucuronides conjugates) that is efficient, effective, and inexpensive is described. this method comprises incubation of the conjugates--after salting-out into ethyl acetate or elution from a c18 cartridge--with anhydrous methanolic hydrogen chloride (methanolysis) for 10 min. it has been successfully applied to our routine radioimmunoassay screening and gc/ms confirmation studies of steroids in prerace and postrace equine urine ... | 1989 | 2610346 |
| reactions of the lectin anti-ahp from the edible snail helix pomatia with n-acetyl-d-galactosamine of streptococci. | the lectin anti-ahp obtained from the albumen gland of the edible snail, helix pomatia, was used for the routine identification of group c streptococci. without any exception all 1,045 strains of group c streptococci (s.equisimilis, and s.zooepidemicus, and s.dysgalactiae) were agglutinated by the helix lectin, but none of the 12,264 strains of group a, 1,346 strains of group g, 330 strains of group b as well as that of other streptococcal groups. the presence of type-specific t antigens of grou ... | 1989 | 2622116 |
| topographical and planar distribution of helix pomatia lectin-binding glycoconjugates in secretory granules and plasma membrane of pancreatic exocrine acinar cells of the rat: demonstration of membrane heterogeneity. | the lectin-gold technique was used to detect helix pomatia lectin (hpl) binding sites directly on thin sections of rat pancreas embedded in lowicryl k4m and on freeze-fractured preparations of rat pancreas submitted to fracture label. on thin sections of acinar cells, whereas the content of zymogen granules was negative or weakly labeled, the limiting membrane displayed a high degree of labeling. in the golgi complex, labeling by hpl was localized on the trans saccules and the limiting membrane ... | 1989 | 2672769 |
| experimental glomerulonephritis induced in rats by a lectin and its antibodies. | the aim of the present study was to investigate the events following interaction of antibody with an antigen planted on the surface of glomerular endothelial cell (gen). a lectin, helix pomatia agglutinin (hpa), was planted at the surface of rat gen by the perfusion of the isolated left kidney with neuraminidase (nrd) and hpa. a subsequent perfusion with igg fraction, but not with fab fragments, of rabbit anti-hpa serum induced formation of granular immune deposits (ids) on the surface of gen. w ... | 1989 | 2689748 |
| lectin-gold cytochemistry of the golgi apparatus in rabbit luteal cells, with special emphasis on the formation of a lysosomal-type membrane. | in rabbit luteal cells embedded in glycolmethacrylate and stained with pta at low ph highly glycosylated membrane patches can be observed after vesiculation of the trans-golgi network. as these membranes could be prelysosomal, their sialic acid content was investigated by post-embedding labeling with limax flavus agglutinin (lfa)/fetuin-au. additional labeling of the golgi apparatus was performed with wheat germ agglutinin (wga)/ovomucoid au, ricinus communis agglutinini (rcai)/au and helix poma ... | 1989 | 2715051 |
| [experimental infection of 2 species of laboratory rodents with invasive larvae of elaphostrongylus cervi (nematoda, metastrongyloidea)]. | single doses (from 300 to 1000 larvi per an animal) of invasive larvae e. cervi cameron, 1931, obtained from experimentally infected snails helix pomatia l. were given to 17 guinea pigs and 17 golden hamsters. clinical nervous symptoms in the form of paresis and paralysis of limbs occurred only in the guinea pigs which were given a dose of 1000 larvi. these animals died in the period from the 75th to 117th day of infection. from their central nervous system single adult males and females of e. c ... | 1989 | 2728450 |
| demonstration of sugar residues in the ultimobranchial tubule and thyroid c-cells of the rat using peroxidase labelled lectins. | the distribution of various sugars, that contribute to the composition of the carbohydrate components of the glycoprotein in the ultimobranchial tubule and the thyroid c-cells has been studied, using eight peroxidase-labelled lectins (dolichos biblorus, glycine max, bauhinia purpurea, helix pomatia, arachis hipogaea, ulex europaeus i, limax flavus and sophora japonica). the results obtained seem to show that there are receptors for dolichos biflorus, glycine max, bauhinia purpurea, helix pomatia ... | 1989 | 2757235 |
| reactivity of visceral yolk sac endoderm with lectins: changes related to age and species. | the expression of receptors for lectins reacting with extraembryonal endoderm was compared between mouse and rat at different stages of gestation. fluorescein-conjugated helix pomatia agglutinin (hpa), dolichos biflorus agglutinin (dba), sophora japonica agglutinin (sja), peanut agglutinin (pna), bandeiraea simplicifolia agglutinin 1 (bsa-1) and gold-conjugated dba and pna were used. it was shown that the rat visceral endoderm does not express the receptors for hpa, while the mouse tissue does. ... | 1989 | 2762737 |
| synthesis and initial processing of gastric apomucin. | the initiation of the processing of apomucin was investigated using mucus glycoprotein synthesizing polysomes from rat gastric epithelial cells. the polysomes were isolated from cells labeled with [3h]palmitic acid and [14c]n-acetylgalactosamine, purified on helix pomatia-sepharose affinity column, dissociated to release peptidyl-trna, and chromatographed on deae-hplc column to separate peptidyl-trna complexes from the free and ribosomal rna and proteins. the analysis of the hplc purified peptid ... | 1989 | 2764978 |
| lectin histochemistry in ulcerative colitis and crohn's disease. | the glycoconjugate composition of intestinal goblet cell mucin was characterized according to the anatomical distribution of lectin-binding sites in surgically resected intestinal tissues and mucosal biopsy specimens obtained from 38 control subjects, and from 32 patients with the active phase of ulcerative colitis, and 12 with crohn's disease. immunoperoxidase labeling studies found that in control tissues binding by soybean agglutinin (sba), dolichos biflorus agglutinin (dba), wheatgerm agglut ... | 1989 | 2769448 |
| biosynthesis and secretion of enamel proteins in the rat incisor. | the synthesis and secretion of enamel proteins (eps) in rat incisors was examined using cytochemical and biochemical methods. radioautography after injection of 3h-methionine showed that ameloblasts in the presecretory, secretory, and maturation stages of amelogenesis actively synthesized and secreted proteins. immunocytochemistry with an antibody to mouse amelogenins revealed the presence of eps in the protein synthetic and secretory organelles of these cells at all three stages. labeling was a ... | 1989 | 2774207 |
| comparison of glycoconjugates at the surface of developing type ii pneumocytes and clara cells. | the apical surface coat of type ii pneumocytes and clara cells in pre- and post-natal rat lung was examined with lectin histochemical methods. lectins from helix pomatia (hpa), peanut (pna) and maclura pomifera (mpa) were conjugated with horseradish peroxidase and used to stain paraffin sections of fixed lung with or without certain pre-treatments. hpa and mpa were observed to react with almost all type ii pneumocytes at postnatal day 1. type ii pneumocytes that stained with a sialidase-pna sequ ... | 1989 | 2777637 |
| detection and isolation of antigen-specific b cells by the fluorescence activated cell sorter (facs). | a method is described for the isolation of antigen-specific b cells from immunized and subsequently boosted mice. antigen-specific b cells were stained by incubation with fluorescein isothiocyanate (fitc)-labelled antigen and then detected and isolated in a fluorescence activated cell sorter (facs). ovalbumin (ova) and helix pomatia haemocyanin (hph) were used as antigens in this procedure, yielding relative amounts of antigen-fitc-binding lymphocytes of 0.9 +/- 0.4% and 3.5 +/- 3.1%. the fitc-p ... | 1989 | 2784157 |
| cytochemical and biochemical characterization of glycoproteins in forming and maturing enamel of the rat incisor. | biochemical and histochemical studies have shown the presence of various carbohydrates in enamel. using lectin-gold cytochemistry, we have examined the distribution of glycoconjugates containing n-acetyl-d-galactosamine (galnac) and/or n-acetyl-glucosamine (glcnac)/n-acetyl-neuraminic acid (neunac) residues in rat incisor ameloblasts and in forming and maturing enamel embedded in lowicryl k4m, lr gold, and lr white resins. the enamel proteins that contain these carbohydrate moieties were further ... | 1989 | 2809173 |
| cytoplasmic intermediate filament proteins of invertebrates are closer to nuclear lamins than are vertebrate intermediate filament proteins; sequence characterization of two muscle proteins of a nematode. | the giant body muscle cells of the nematode ascaris lumbricoides show a complex three dimensional array of intermediate filaments (ifs). they contain two proteins, a (71 kd) and b (63 kd), which we now show are able to form homopolymeric filaments in vitro. the complete amino acid sequence of b and 80% of a have been determined. a and b are two homologous proteins with a 55% sequence identity over the rod and tail domains. sequence comparisons with the only other invertebrate if protein currentl ... | 1989 | 2583097 |
| isolation and identification of 6-desmethylnaproxen sulfate as a new metabolite of naproxen in human plasma. | a new metabolite of naproxen, 6-desmethylnaproxen sulfate (6-dmns), has been identified in plasma from normal and uremic subjects after oral administration of a single 500 mg dose of naproxen. tentative identification was achieved by the finding of an increase in the concentration of 6-dmn upon incubation of the plasma with arylsulfatase from helix pomatia or from aerobacter aerogenes. more definitive identification was established through demonstration that the hplc retention time of the conjug ... | 1989 | 2566468 |
| comparative analysis of agglutinins from hemolymph and albumin gland of helix pomatia. | the hemolymph of helix pomatia contains a weak agglutinating activity. this lectin concentration was calculated to be about 1.8 micrograms.ml-1. among the different red blood cells tested, pronase-treated sheep erythrocytes were found to be the most suitable indicator cells. their agglutination could be inhibited by galnac and glcnac. the serum agglutinin was isolated by affinity chromatography using sephadex g-200 as the matrix. it exhibited a single band in discontinuous page. in the presence ... | 1989 | 2808854 |
| saccharide residues in human gingiva as revealed with fluorochrome-coupled lectins. | the histochemical binding of 16 fluorochrome-conjugated lectins to human marginal gingiva was investigated. of a total of 14 galactose/n-acetylgalactosamine (gal/galnac)-specific lectins, dolichos biflorus (dba), helix pomatia (hpa), and helix aspersa agglutinins (haa) were blood group a-reactive whereas griffonia simplicifolia i-b4 (gsa-i-b4) and sophora japonica (sja) agglutinins were blood group b-reactive. hpa, haa and gsa-i-b4 bound to all suprabasal epithelial cells and to vascular endothe ... | 1989 | 2542513 |
| cytochemical demonstration of lectin binding-sites in the cuticle and tissues of acanthocheilonema viteae (filarioidea). | the lectin-gold technique was used for the ultrastructural localization of lectin binding sites on thin sections of lowicryl k4m embedded adult females, infective larvae and sds-2-mercaptoethanol-insoluble cuticle components of acanthocheilonema (dipetalonema) viteae. helix pomatia lectin (hpl) coupled to 14 nm gold particles, was used for the demonstration of n-acetyl-d-galactosamine-containing glycoconjugates. triticum vulgaris (wheat germ) agglutinin (wga) coupled to 10 nm gold particles afte ... | 1989 | 2566259 |
| tn epitope (n-acetyl-d-galactosamine alpha-o-serine/threonine) density in primary breast carcinoma: a functional predictor of aggressiveness. | this interpretive review attempts to dovetail advanced work by different groups of investigators on blood group and carcinoma (ca) glycoconjugates that have terminal, immunoreactive tn epitopes (galnac alpha-o-ser/thr), and on the interaction of those structures with complementary antibodies and lectins. fenlon et al. (1987) and leathem and brooks (1987) found a positive correlation between primary breast ca aggressiveness and its affinity for helix pomatia (hpa) lectin. this phenomenon was used ... | 1989 | 2467192 |
| physicochemical properties of a human glycoprotein bearing blood group a activity. | a human erythrocyte glycoprotein was isolated and purified from blood of group a+1 by a procedure involving chloroform-methanol extraction and affinity chromatography on helix pomatia lectin-sepharose 6mb, and some of its physicochemical properties were determined. the resulting preparation was homogeneous as indicated by polyacrylamide gel electrophoresis. the glycoprotein contained nearly 60% carbohydrate and 40% protein. it was water-soluble and inhibited the agglutination of a-erythrocytes. ... | 1989 | 2613683 |
| rapid detection of herpes simplex virus with fluorescein-labeled helix pomatia lectin. | the use of fluorescein-conjugated helix pomatia lectin was shown to be as effective as fluorescein-conjugated monoclonal antibody reagents for the detection and differentiation of herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2) in mrc-5 cell culture. cells infected with hsv-1 generally displayed a pattern of nongranular or diffuse fluorescence, while cells infected with hsv-2 were identified by the production of fluorescent grains and flecks. this unique nonimmunological reagent, when u ... | 1989 | 2545739 |
| mitogenic lectins bind to the antigen receptor on human lymphocytes. | the specificity of interactions between mitogenic and non-mitogenic lectins and disulfide-linked cell surface receptors on human lymphocytes was explored. lysates (nonidet-p40) of surface-radioiodinated tonsil lymphocytes and t lymphoblastoid cells (hpb-all) were absorbed with lectin-agarose derivatives (or bovine serum albumin, bsa-agarose) or immunoprecipitated with appropriate monoclonal antibodies (mab). lectin eluates and solubilized immunoprecipitates were analyzed by two-dimensional (nonr ... | 1989 | 2703017 |
| the reaction of nitrogen monoxide with the haemocyanins of the crayfish astacus leptodactylus and the snail helix pomatia. | the rate of the reaction of astacus leptodactylus methaemocyanin with no follows the henderson-hasselbalch equation with a pka of 5.85, suggesting that one imidazole ligand of cu was exchanged for no. the reaction is blocked by f- as a bridging ligand. the same imidazole residue might be responsible for the decomposition of nitrosylhaemocyanin, [cu1no+cuii], with an unlocated binding site for no, into methaemocyanin and no, as the rate increase with ph. no could react preferentially with cua of ... | 1989 | 2818567 |
| the reaction of mercaptans with tyrosinases and hemocyanins. | 1. titration of neurospora tyrosinase with 2-mercaptoethanol shows that the increase of absorbance at 700 nm is directly correlated to the loss of enzymatic activity. approximately 2 mol of 2-mercaptoethanol per mole of protein are needed for full development of the green, enzymatically inactive complex. the increase of absorbance at 700 nm is also proportional to the intensity of the epr signal and the amount of non-covalently bound 2-[35s] mercaptoethanol to the enzyme. the maximal epr intensi ... | 1989 | 209826 |
| the reaction of nitrite with the haemocyanin of the roman snail (helix pomatia). | the reaction of nitrite at ph 5.0-7.0 with the deoxyhaemocyanin of a mollusc, the roman snail (helix pomatia), yielded nitrosylhaemocyanin (cuia.no+ cuiib), in contrast with the formation of methaemocyanin with the deoxyhaemocyanin of the crustacean astacus leptodactylus (mud crayfish). with helix haemocyanin 1 no was thereby liberated per active site, as shown by m.s., as against 2 no with astacus haemocyanin. helix nitrosylhaemocyanin was characterized in c.d. by the negative extremum at 336 n ... | 1990 | 2244878 |
| influence of lectins on female sex pheromone reception byheterodera schachtii (nematoda: heteroderidae) males. | a bioassay was developed in which the chemotactic behavior of males of the sugarbeet cyst nematodeheterodera schachtii towards the female sex pheromone can be observed and documented at any time. the standardized test, performed under sterile conditions, gives reproducible results within 1-2 hr. incubation of males with the lectins fromcanavalia ensiformis, triticum vulgare, arachis hypogaea, helix pomatia, andlimax flavus did not affect chemotactic attraction to the sex pheromone. supplementing ... | 1990 | 24264204 |
| transient expression of type 2 chain in a-active hexaglycosylceramide of rat small intestine at weaning time. demonstration by affinity chromatography and ceramide glycanase hydrolysis of a-active glycosphingolipids followed by gas chromatography and mass spectrometry of permethylated hexasaccharides. | the small intestine of 15- to 23-day-old rats was cut into four segments from the duodenum to the ileum. neutral glycosphingolipids were purified from each segment and submitted to thin-layer chromatography and immunostaining with the a005 monoclonal anti-a antibody. this antibody detected an hexaglycosylceramide located mainly in the duodenum during the postnatal development. in order to characterize hexaglycosylceramides, blood group a-active glycolipids were purified by affinity chromatograph ... | 1990 | 2221915 |
| helix pomatia agglutinin binds specifically to the golgi apparatus in cultured human fibroblasts and reveals two golgi apparatus-specific glycoproteins. | fluorochrome-coupled helix pomatia agglutinin (hpa), but not other lectin-conjugates with the same nominal specificity, bound specifically to the golgi apparatus in cultured human fibroblasts, revealing a cytoplasmic juxtanuclear reticular structure. unlike other golgi-binding lectins the hpa-conjugates did not bind to the cell surface membrane or pericellular matrix. experiments with 35s-methionine-labeled cells showed that hpa recognized two glycoproteins of mr 170,000 and 400,000 among the se ... | 1990 | 2228736 |
| heterogeneity of the blood group abh antigens and variation in the expression of these antigens of secretory granules in human cervical glands. an electron microscopic observation using lectins and monoclonal antibodies. | cytochemical localization of blood group abh antigens was examined in secretory cells of human cervical glands by application of a post-embedding lectin-gold as well as immuno-gold labeling procedure using monoclonal antibodies. blood group specific lectins such as dolichos biflorus agglutinin (dba), helix pomatia agglutinin (hpa), griffonia simplicifolia agglutinin i-b4 (gsai-b4) and ulex europaeus agglutinin-i (uea-i) reacted with secretory granules but not with other cytoplasmic organellae su ... | 1990 | 2283312 |
| binding of the blood group-reactive lectins to human adult kidney specimens. | the binding of a panel of blood group-reactive lectins to frozen sections of human kidney was studied with a special emphasis on reactivity with endothelia and basement membranes. the blood group a-reactive lectins, all specific for alpha-d-n-acetylgalactosamine (galnac), helix aspersa (haa), helix pomatia (hpa), and griffonia simplicifolia i-a4 (gsa-i-a4) agglutinins bound to the endothelium in specimens with blood groups a and ab. in other samples, these lectins reacted predominantly with tubu ... | 1990 | 2297076 |
| ultrastructural comparative distribution of carbohydrates in human tracheal and frog palate mucosa using neuraminidase and lectin-colloidal gold complexes. | we have compared, at the ultrastructural level, the carbohydrate structure of glycoconjugates of the different types of secretory cells of the human tracheal mucosa (htm) and the frog palate mucosa (fpm), proposed as a model for studying bacterial adherence to mucus-coated respiratory epithelium. in addition to reactivity with concanavalin a and lens cullinaris agglutinin, reactivity of epon-embedded htm and fpm secretory granules was studied by transmission electron microscopy using neuraminida ... | 1990 | 2311103 |
| identification of glucose conjugates as major urinary metabolites of cannabidiol in the dog. | 1. three dogs were treated i.v. with cannabidiol (cbd) and urine collected at intervals to 30 h. 2. metabolites were extracted, converted into trimethylsilyl (tms) derivatives and examined by g.l.c.-mass spectrometry. 3. the major metabolites excreted at early times were identified as the phenol glucosides of 4"-hydroxy-cbd, 5"-hydroxy-cbd and 6-oxo-cbd. 4. these three oxidized metabolites were not found unconjugated, and none of the free oxidized metabolites in urine were found conjugated with ... | 1990 | 2333714 |
| ultracytochemistry of glycoconjugates in rat brunner's gland with labeled lectins. | mucous glycoproteins of rat brunner's gland were examined ultracytochemically to elucidate the intracellular localization and sites of glycosylation in relation to the functional polarity of the cell organellae using a combination of hydrophilic resin embedment and postembedding staining with labeled lectins. a few cis cisternae were stained with hpa (helix pomatia agglutinin, specific for terminal alpha-n-acetylgalactosamine) and dba (dolichos biflorus aggulutinin, specific for terminal alpha-n ... | 1990 | 2358769 |
| lectin cytochemistry reveals differences between hamster trachea and bronchus in the composition of epithelial surface glycoconjugates and in the response of secretory cells to neutrophil elastase. | hamsters exposed to an intratracheal instillation of human neutrophil elastase (hne) accumulate an abnormally high number of secretory granules in bronchial but not tracheal epithelial cells. we employed lectin cytochemistry to investigate possible differences in the epithelial cell surface glycoconjugate layer in trachea compared to bronchus which might explain the regional dissimilarity in response to hne. portions of glutaraldehyde-fixed trachea and bronchi were incubated in one of several fe ... | 1990 | 2363936 |
| glycoconjugate distribution in the human fundic mucosa revealed by lectin- and glycoprotein-gold cytochemistry. | the glycoconjugates of the human fundic mucosa were characterized at the ultrastructural level by means of direct (helix pomatia agglutinin-gold complex) and indirect lectin techniques (concanavalin a and horseradish peroxidase-gold complex; wheat germ agglutinin and ovomucoid-gold complex). surface mucous cells and mucous neck cells secreted o-glycoproteins with n-acetylgalactosamine and n-acetylglucosamine residues at the non reducing terminus of the saccharidic chain. the secretory granules o ... | 1990 | 2081692 |
| characterization of galactose-containing glycoconjugates in the human retina: a lectin histochemical study. | seven specimens of morphologically normal formalin-fixed and paraffin-embedded human retina were studied using a panel of fourteen biotinylated lectins, all of which react with glycoconjugates containing galactose and n-acetylgalactosamine residues. agaricus bisporus (aba), bauhinia purpurea (bpa), phaseolus vulgaris (pha-e), peanut (pna) and ricinus communis (rca-i) agglutinins labeled photoreceptor cells prior to enzymatic predigestion. bpa and pna bound specifically to cones. the plexiform la ... | 1990 | 2091899 |
| use of peldri ii as a sublimation dehydrant in place of critical-point drying in fracture-label cytochemistry and in backscattered electron imaging fracture-label. | peldri ii, a fluorocarbon compound solid at room temperature, was used as a sublimation dehydrant in place of critical-point drying (cpd) in freeze-fracture cytochemistry. its applicability to the fracture-label replica method was demonstrated using freeze-fractured rat pancreas labeled with helix pomatia lectin-gold complex and phospholipase a2-gold complex for the detection of glycoconjugates and phospholipids, respectively. no difference in morphology and labeling pattern was detected in cpd- ... | 1990 | 2153786 |
| flow cytometric analysis of cell surface carbohydrates in metastatic human breast cancer. | helix pomatia agglutinin (hpa)- and concanavalin a (con a)-binding carbohydrate expression were studied on 32 tumour samples from primary adenocarcinoma of the breast and 12 samples from lymph node metastases. live cells were spilled from each of the fresh samples and the extent of fluorescent-labelled hpa and con a-binding was assessed by flow cytometry. the extent of brightness was expressed in a defined quantitative fashion and the percentage of positive cells was accurately determined from a ... | 1990 | 2167120 |
| time courses of lidocaine effects on sodium membrane currents in small and large neurons. | time courses of effects of lidocaine on sodium currents and sodium dependent action potentials were studied in somata of small and large neurons. cultured rat sensory spinal ganglion cells (diameter: 30 microns) and neurons of the buccal ganglion of helix pomatia (diameter: 150 microns) served as the test cells. the latency of the suppressive action of lidocaine was the longer the larger the of the cells was. maximal blocking effects occurred within 10 min in sensory spinal ganglion cells and wi ... | 1990 | 2177020 |
| effects of alcohol on lectin binding affinity in rat gastric mucosa. | fluoresceinated lectins were employed to qualitatively evaluate cell surface carbohydrates, with and without ethanol exposure, in rat stomach mucosae. rats received 1 ml of saline, or 50% or 100% ethanol orally. after 30 min, tissue samples of the glandular stomach were retrieved, cryosectioned, and incubated with one of a panel of lectins. another set of sections was preincubated with neuraminidase to remove sialic acid residues. qualitative evaluation of lectin binding showed that although sev ... | 1990 | 2194768 |
| [determination of lectin receptors on the surface membranes of lymphoid cells]. | the authors describe a method for the detection of carbohydrate determinants contained by the cellular surface membrane glycosylated biopolymers, based on the use of horse radish peroxidase-conjugated arachis, helix pomatia, ricinus communis, lens, glycine max, sophora japonica lectins. the pattern of lectin receptor distribution on normal human blood lymphocytes, tonsillar lymphoid cells, and in some forms of leukemia and lymphoma is shown. the authors consider it essential that a lectin kit be ... | 1990 | 1692358 |
| lectin binding patterns to terminal sugars of rat lung alveolar epithelial cells. | we used post-embedding cytochemical techniques to investigate the lectin binding profiles of rat lung alveolar epithelial cells. sections from rat lung embedded in the hydrophilic resin lowicryl k4m were incubated either directly with a lectin-gold complex or with an unlabeled lectin followed by a specific glycoprotein-gold complex. the binding patterns of the five lectins used could be divided into three categories according to their reactivity with alveolar epithelial cells: (a) the limax flav ... | 1990 | 1688898 |
| a plasma membrane antigen of rat glomerular epithelial cells. antigenic determinants involving n-linked sugar residues in a 140-kilodalton sialoglycoprotein of the podocytes. | the aim of the present study was to investigate the antigenic make up of the plasma membrane of rat glomerular visceral epithelial cells (gep). a crude plasma membrane fraction (pm) was extracted by 1% sodium dioxycholate from isolated rat glomeruli. pm was digested with neuraminidase (nrd) and purified by the helix pomatia agglutinin (hpa)-affinity column. when studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the hpa-affinity purified plasma membrane fraction (hpa-pm) forme ... | 1990 | 1700199 |
| interaction of bluetongue virus with bovine lymphocytes. | freshly isolated, and established, cultures of bovine peripheral blood mononuclear leukocytes (pbmls) were exposed to bluetongue virus (btv) for the purpose of defining potential lymphotropism. pbml cultures were established in the presence of interleukin 2 (il-2) and mitogen and maintained either as bulk culture or were cloned prior to infectivity studies. all cultures appeared to be of the t cell phenotype based on the following characteristics: binding of t lymphocyte-specific lectins (i.e. p ... | 1990 | 2155289 |
| changes in glycoconjugates revealed by lectin staining in the developing airways of syrian golden hamsters. | lectin binding was studied in the developing airways of syrian golden hamsters on gestational days 11-16 (day 16 is the day of birth). the trachea and lungs were fixed in 4% formaldehyde-1% glutaraldehyde, 6% mercuric chloride-1% sodium acetate-0.1% glutaraldehyde, and 95% ethanol; embedded in paraffin; and stained with eight lectin-horseradish peroxidase conjugates: triticum vulgare (wga), dolichos biflorus (dba), helix pomatia (hpa), maclura pomifera (mpa), griffonia simplicifolia i-b4 (gsa i- ... | 1990 | 1700650 |
| characterization of the high mr glycoprotein (gp300) of equine herpesvirus type 1 as a novel glycoprotein with extensive o-linked carbohydrate. | the high mr glycoprotein (gp300) of equine herpesvirus type 1 was found to have an mr, estimated by sds-page, of over 400,000 and was confirmed as being a surface glycoprotein by 125i-labelling. in contrast to [3h]glucosamine, gp300 showed very low levels of [3h]glucosamine, gp300 showed very low levels of [3h]mannose incorporation. the mr of gp300 showed no detectable change upon treatment of purified virus with n-glycanase, and showed only a small change in virus-infected cells treated with tu ... | 1990 | 2172454 |
| difference in the ability of blood group-specific lectins and monoclonal antibodies to recognize the abh antigens in human tissues. | twelve different kinds of blood group-specific lectins have been used along with monoclonal anti-a, -b and -h antibodies for detecting the corresponding antigens in selected human tissues. although most of the lectins recognized the antigens in the tissue sections examined, they displayed marked differences in their recognition patterns in certain tissues. helix asparsa agglutinin (haa), helix pomatia agglutinin (hpa) and monoclonal anti-a antibody recognized a antigens in the mucous cells of sa ... | 1990 | 1705925 |
| [fine structure studies and identification of lectin receptors on the viral envelope of two hiv-isolates]. | the electron microscopic particle findings were compared with the levels of revertase in corresponding samples over a longer period of time, and a good correlation was found. comparative investigations of the fine-structure of two hiv isolates did not reveal any morphological differences. it can be assumed, on the basis of the comparative studies on lectin receptors using helix pomatia lectin, that the viral envelopes of the two isolates are equipped similarly with n-acetyl-d-galactosamine. the ... | 1990 | 1706878 |
| [study of lymphocyte subpopulations using peanut, soybean and snail lectins]. | the method of penning in plastic dishes was to isolate the pna+ and pna- cell populations from the tonsil tissue. carbohydrate determinants of lymphoid cell surface membranes being at varying stages of antigen-independent and antigen-dependent differentiation have been studied with the use of peanut, soya and edible snail lectins conjugated with horse radish peroxidase. | 1990 | 2253858 |
| surface array proteins of campylobacter fetus block lectin-mediated binding to type a lipopolysaccharide. | campylobacter fetus strains with type a lipopolysaccharide (lps) and a surface array protein layer (s+) have been found to be pathogenic in humans and animals. spontaneous laboratory mutants that lack surface array proteins (s-) are sensitive to the bactericidal activity of normal human serum. the ability of lectins to determine the presence of the s-layer and differentiate lps type was assessed. we screened 14 lectins and found 3 (wheat germ agglutinin, bandeiraea simplicifolia ii, and helix po ... | 1990 | 2387622 |
| defining the specificity of anti-a/b igm/g antibodies with different antigens and lectins. | the specificity of anti-histo blood group a/b antibodies is defined by the antigen used for their production and selection (in the case of mouse monoclonal antibodies, mabs) or by the antigens present on various intestinal bacteria (for polyclonal human igm and/or igg, phabs). absorption experiments with red blood cells and free antigen have been used earlier to define specificity; here we use a/b substances of human, animal and synthetic origin as well as known reactivities of mabs or lectins a ... | 1991 | 1862641 |
| a 58-kda resident protein of the cis golgi cisterna is not terminally glycosylated. | a 58-kda golgi protein (gp58) was previously identified and found to be concentrated in cis golgi cisternae in several cell types (saraste, j., palade, g.e., and farquhar, m.g. (1987) j. cell biol. 105, 2021-2029). in this study the protein was partially purified from rat pancreas and mouse myeloma cells in order to characterize its oligosaccharides. it migrated on sodium dodecyl sulfate-polyacrylamide gels as a 57-58-kda doublet under reducing conditions or as a single approximately 116-kda ban ... | 1991 | 1894639 |
| biosynthesis in vitro of glca beta 1-3nlcose4cer by a novel glucuronyltransferase (glcat-1) from embryonic chicken brain. | a novel glucuronyltransferase (glcat-1) has been detected in embryonic chicken brains. this enzyme catalyzes the biosynthesis in vitro of glucuronic acid containing glycolipids starting from neolactotetraosylceramide (nlcose4cer) and neolactohexaosylceramide (nlcose6cer). the activity is present primarily in the golgi-rich membrane fraction and can be extracted (60%) from the membrane using a neutral detergent, nonidet p-40, at ph 7.0. the detergent-solubilized glcat-1 is stable (70%) at -20 deg ... | 1991 | 1900517 |
| glycoconjugates in the human fetal endolymphatic sac as detected by lectins. | the distribution of glycoconjugates in the 11 to 16 weeks old human fetal endolymphatic sac (es) was analyzed using six biotinylated lectins; wheat germ agglutinin (wga), abrus precatorius agglutinin (apa), ulex europaeus agglutinin i (uea-i), ricinus communis agglutinin 120 (rca120), helix pomatia agglutinin (hpa), concanavalin a (cona). in the 11 week old human fetus, fluorescent reactions with wga, apa, rca120 and cona were detected in the es. there was almost no reaction with hpa and uea-i. ... | 1991 | 1919336 |
| decreased biosynthesis of forssman glycolipid after retinoic acid-induced differentiation of mouse f9 teratocarcinoma cells. lectin-affinity chromatography of the glycolipid-derived oligosaccharide. | glycolipids synthesized by the mouse teratocarcinoma f9 cells and f9 cells (ra/f9 cells) induced to differentiate by a 3-day treatment with 0.1 microm all-trans-retinoic acid were analyzed. both f9 cells and ra/f9 cells were incubated in media containing either d-[6-3h]galactose or d-[6-3h]glucosamine; the metabolically-radiolabeled glycolipids were isolated and the oligosaccharides were released from the glycolipids by ozonolysis and alkali fragmentation. from both cells, a single major pentasa ... | 1991 | 1933935 |
| metabolism and fate of ecdysteroids in the nematodes ascaris suum and parascaris equorum. | when injected with [3h]ecdysone and maintained in vitro, the parasitic nematodes, ascaris suum and parascaris equorum each produced a series of polar and relatively apolar metabolites. a. suum metabolised the compound into ecdysonoic acid ([3h]eoic), ecdysone 25-glucoside ([3h]e25gluc), putative ecdysone 22-phosphate ([3h]e22p) and a series of at least six relatively apolar metabolites. all of these, except ecdysonoic acid, were hydrolysed by a crude enzyme preparation from helix pomatia, releas ... | 1991 | 1944416 |
| aluminofluorides and beryllofluorides as inhibitors of sulphatases. analogues of hydrogen sulphate? | the inhibition of fluoride of sulphatase a from ox liver and of the sulphatases of helix pomatia and aspergillus oryzae is decreased by edta and increased by al3+ or be2+, implicating aluminofluorides and beryllofluorides in the reaction. the inhibition, which is reversible, takes several minutes to develop fully and, at least for the sulphatase of h. pomatia, is of a non-linear mixed competitive-non-competitive type. it is suggested that the aluminofluorides and beryllofluorides are acting as a ... | 1991 | 1953634 |
| the adsorption of bacteria to immobilized lectins. | the agglutination of a selection of bacteria by some lectins was examined. the lectin from codium fragile agglutinated seven strains of salmonella typhimurium. the lectin from helix pomatia agglutinated eight of 12 strains of listeria monocytogenes and a further two strains gave a weak agglutination reaction. helix pomatia lectin conjugated to magnetic microspheres enabled the adsorption of l. monocytogenes from suspension with subsequent elution by the competing ligand n-acetyl galactosamine. a ... | 1991 | 1955420 |
| expression of blood group-related antigens and helix pomatia agglutinin in malignant pleural mesothelioma and pulmonary adenocarcinoma. | in an attempt to differentiate malignant pleural mesothelioma from pulmonary adenocarcinoma by histochemical and immunohistochemical means, the glycoconjugate profiles of five reactive mesothelial lesions, 29 mesotheliomas (20 epithelial, three biphasic, and six fibrous types), and 38 well-differentiated pulmonary adenocarcinomas (34 papillary, two tubular, and two bronchioloalveolar types) were tested with abh blood group-related antigens (bgr-ag) antibody and helix pomatia agglutinin (hpa) whi ... | 1991 | 2001875 |
| experimental epilepsy in vitro: neuromodulating activity of anti-brain autoantibodies from rats exposed to electroconvulsive shock. | neuromodulating activity of anti-brain autoantibodies obtained from electroshocked (ecs) rats was tested on the neurons of isolated suboesophageal ganglion of the snail helix pomatia. in 16 out of 18 spontaneously active (pacemaker) neurons, ecs igg containing anti-brain autoantibodies induced short-lasting epileptiform discharges and membrane depolarization. membrane input resistance and time constant decreased, while membrane capacitance increased after addition of ecs igg. amplitude of evoked ... | 1991 | 1723059 |
| cell type-specific lectin staining of the tracheobronchial epithelium of the rat: quantitative studies with griffonia simplicifolia i isolectin b4. | we compared lectin staining patterns to cell population densities, as determined by morphological criteria in rat airways. eight lectins were studied: griffonia simplicifolia i isolectin b4 (gsi-b4), arachis hypogaea (pna), wisteria floribunda (wfa), glycine maximus (sba), dolichos biflorus (dba), helix pomatia (hpa), ulex europaeus (uea-1), and maclura pomifera (mpa). two of the lectins strongly stained morphologically distinct cell subpopulations. gsi-b4 stained basal cells, and mpa stained no ... | 1991 | 1701188 |
| binding of platelet agglutinating protein p37 from the plasma of a patient with thrombotic thrombocytopenic purpura to human platelets. | we have previously reported the purification of a 37 kda platelet agglutinating protein (pap p37) from the plasma of a patient with thrombotic thrombocytopenic purpura (ttp) that was shown to be present in a subset of ttp patients. to gain further insight into the interaction between pap p37 and platelets, we have studied the properties of pap p37 binding to platelets. washed human platelets from two normal donors and two ttp patients after recovery were used for the experiments. the pap p37 bin ... | 1991 | 2024244 |
| phosphofructokinase from mollusc muscle is activated by phosphorylation. | phosphofructokinase was purified from muscle tissue of two different molluscs, edible snails, helix pomatia (gastropoda), and mussels, mytilus edulis (bivalvia). under denaturing conditions, both enzymes had a molecular mass of 82 kda. in the presence of atp-mg2+, the enzymes were rapidly phosphorylated in vitro by the catalytic subunit of cyclic amp (camp)-dependent protein kinase purified from snail muscle and also by the c subunit of protein kinase from bovine heart. the extent of phosphoryla ... | 1991 | 1654810 |