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an isoenzyme marker possibly associated with the susceptibility of biomphalaria glabrata populations to schistosoma mansoni.nine laboratory populations and one field population of the snail host biomphalaria glabrata were compared with respect to their electrophoretic patterns for acid phosphatase (acp) and with their susceptibility to schistosoma mansoni infection. a strong correlation (r = 0.98) was noted between the frequency of the isoenzymes acp2-s and acp2-f observed in the populations and the level of snail susceptibility as determined by bioassay. the isoenzyme acp2-s was associated with susceptibility, acp2- ...19816123248
concanavalin a-induced receptor redistribution on biomphalaria glabrata hemocytes: characterization of capping and patching responses. 19817024423
genetic variation among laboratory strains of the planorbid snail biomphalaria glabrata.isozymes of laboratory strains of biomphalaria glabrata have been studied by starch gel electrophoresis. methods are outlined for adaptation of this technique to the genetic study of these snails. twenty-eight presumptive gene loci have been identified. twelve invariant enzymes were observed. sixteen loci displayed some polymorphism within or among the strains. these polymorphisms were generally widespread among strains from brazil, puerto rico, st. lucia, and the dominican republic. a high degr ...19817337694
the behavior of biomphalaria glabrata (gastropoda: pulmonata) hemocytes following exposure to lectins. 19817324324
distribution and variation of hemagglutinating activity in the hemolymph of biomphalaria glabrata. 19817320518
comparison of concanavalin a-reactive determinants on hemocytes of two biomphalaria glabrata snail stocks: receptor binding and redistribution. 19816165627
biogenic amine metabolism in biomphalaria glabrata-ii. monoamine oxidase activity. 19816116567
lack of resistance of the snail biomphalaria glabrata after nine years of exposure to bayluscide. 19817268836
properties of glycogen synthase and phosphorylase from biomphalaria glabrata (mollusca).glycogen synthase and phosphorylase were characterized from the cephalopedal region of the snail, biomphalaria glabrata. glycogen synthase exhibited increases and decreases in its activity ratio (-g6p/+g6p) under conditions that are known to cause conversion of the two forms of the enzyme from mammalian systems, implying that the snail's synthase also possesses interconvertible forms. each form had a distinct ph optimum, with the g6p-independent form (synthase alpha) exhibiting maximum activity ...19826804613
molluscicidal properties of various saponins.a series of 24 different saponins isolated from various medicinal plants have been tested against biomphalaria glabrata, one of the snail vectors of schistosomiasis (bilharziasis). in general, monodesmosidic triterpenoid saponins exhibit strong molluscicidal activity whereas bidesmosidic saponins as well as the aglycones are fully inactive. some dammarane glycosides and alkaloidal saponins have also been tested.198217402079
lectin-induced mitogenesis of cytotoxic amebae (nuclearia) isolated from biomphalaria glabrata (mollusca:gastropoda). 19827095235
lectin-induced modulation of snail hemocyte surface determinants: clearance of con a-receptor complexes.the fate of surface concanavalin a (con a)-receptor complexes on hemocytes from two stocks of the snail, biomphalaria glabrata (pr albino and 10-r2), was studied using both direct (con a-fitc) and indirect (biotin-con a, avidin-fitc) fluorescent staining methods. the results of experiments in which living hemocytes were exposed first to biotin-con a, followed by fixation and subsequent treatment with avidin-fitc to localized surface-bound lectin, demonstrate that con a complexes are rapidly clea ...19826182034
anacardic acid: molluscicide in cashew nut shell liquid.the components of anacardic acid, (a mixture of 6-n-c (15) alkylsalicylic acids whose side chains vary in degrees of unsaturation) have been isolated by high pressure liquid chromatography from a crude extract of cashew nut shell, anacardium occidentale, and tested for toxicity to fresh water snails, biomphalaria glabrata. the triene component is the most toxic form (lc (50) 0.35 ppm), the diene and monoene components are less toxic (lc (50) 0.9 and 1.4 ppm), and the saturated component is relat ...198217402106
ribeiroia marini: irradiated miracidia and induction of acquired resistance in biomphalaria glabrata. 19827056343
concanavalin a-mediated phagocytosis of yeast by biomphalaria glabrata hemocytes in vitro: effects of temperature and lectin concentration. 19827045238
evaluation of chemotherapy in the control of schistosoma mansoni in marquis valley, saint lucia. ii. biological results.following the final chemotherapy campaign of a 4-year program, the biological assessment of transmission of schistosoma mansoni was evaluated for 5 years. no infected biomphalaria glabrata were found amongst 28,791 and 13,550 snails examined from flowing and static index sites, respectively. however, 89 infected snails of 16,602 examined (0.54%) were found from additional sites. sentinel snails examined between march 1974 and october 1977 revealed no infections and their use was discontinued. fo ...19827058972
ultrastructure of encapsulation of schistosoma mansoni mother sporocysts by hemocytes of juveniles of the 10-r2 strain of biomphalaria glabrata.dynamic aspects of the cellular responses of juvenile (2-3 mm shell diameter) 10-r2 strain biomphalaria glabrata to newly penetrated schistosoma mansoni (nih-sm-pr-1 strain) were studied at the ultrastructural level. as early as 3 hr postexposure (pe), host hemocytes had contacted the parasite's surface and by 7.5 hr, had phagocytosed sporocyst microvilli and small pieces of underlying tegument. most sporocysts observed at 24 hr pe lacked tegumental cytoplasm, and germinal cells and other intern ...19827077450
predation of the snail biomphalaria glabrata by freshwater shrimps in st. lucia, west indies. 19827082076
further characterization of acquired resistance in biomphalaria glabrata.the albino strain of biomphalaria glabrata is capable of developing an acquired resistance to echinostomes that is relatively specific, i.e., strong against echinostoma lindoense, challenged by the homologous species, moderate or weak against closely related e. paraensei and e. liei, and nonexistent against paryphostomum segregatum and schistosoma mansoni. the present results show that acquired resistance induced by e. paraensei is much stronger against the heterologous e. lindoense than against ...19827119983
swellengrebel lecture: survival of schistosoma mansoni and other trematode larvae in the snail biomphalaria glabrata. a discussion of the interference theory. 19827123644
in vitro encounters between schistosoma mansoni primary sporocysts and hemolymph components of susceptible and resistant strains of biomphalaria glabrata.encounters between axenically transformed sporocysts of the puerto rican 1 strain of schistosoma mansoni and hemocytes and plasmaaa components of susceptible "m line" and resistant 10-r2 and 13-16-1 strains of biomphalaria glabrata were studied in vitro. after 24 hours of incubation in susceptible hemolymph components, 94.4% of 36 observed sporocysts retained normal structure, whereas 9l.6% of 48 sporocysts were destroyed after 24 hours of incubation in resistant hemolymph components. sporocysts ...19827125067
[evaluation of 10 years of research on s. mansoni schistosomiasis a in the french west indies].from 1971 until 1982, we studied the ecological factors which stead fast a perennial schistosomiasis in guadeloupe on an exhaustive mode. more recently, we start a similar investigation in martinique. in guadeloupe the single vector of schistosoma mansoni is the snail biomphalaria glabrata which is an ubiquist species. but the transmission to man is restricted to the "canal-sewers" where faeces are dropped and not in the rivers of basse-terre or the ponds of grande-terre. moreover, the infection ...19827126776
amebocytic accumulations in biomphalaria glabrata: fine structure.internal pathologic conditions in four stocks of biomphalaria glabrata involve amebocytes. most snails exhibiting these conditions are also nonsusceptible to infection with schistosoma mansoni. fine structure studies reveal that periaortic and atrial amebocytic accumulations show two responses that involve heart tissue: a generalized response in which amebocytes have infiltrated the myocardium and focal areas of nodules in which heart tissue is encapsulated and destroyed. in pericardial accumula ...19827128891
transmission of schistosoma mansoni from man to snail: laboratory studies on the influence of snail and miracidial densities on transmission success.the relationship between infection success, the density of schistosoma mansoni miracidia and the density of the snail host biomphalaria glabrata was examined experimentally. within laboratory infection arenas, the distribution of miracidial infections/snail was approximately uniform and the net rate of snail infection was directly positive binomial to miracidial density. in contrast, however, the rate of infection declined exponentially as snail density increased. mathematical models are develop ...19827145477
population structure in biomphalaria glabrata: examination of an hypothesis for the patchy distribution of susceptibility to schistosomes.it has been suggested that the patchy distribution of snail susceptibility to schistosomes might be due to genetic drift. since direct estimation of genes for susceptibility is not feasible in population studies, electrophoretic techniques were used to examine this hypothesis for populations of biomphalaria glabrata from puerto rico. these populations were characterized by relative genetic homogeneity within populations and differentiation among local populations. the data presented are consiste ...19827149103
effects of starvation and infection with schistosoma mansoni on the release rate of free amino acids (faa) by biomphalaria glabrata.1. the elimination of dissolved free amino acids (faa) by individual snails during a period of 2 hr was measured. the faa concentration in the hemolymph and in the homogenate also was determined. 2. for uninfected, normally fed snails a daily faa production of 0.7 +/- 0.25 mumol glycine equivalents/g live wt was found. with snails starving for 7 days the daily faa release rate fell to a mean value of 0.44 +/- 0.19 mumol glycine equivalents/g live wt. infection with schistosoma mansoni, however, ...19827172633
use of praziquantel in patients with schistosomiasis mansoni previously treated with oxamniquine and/or hycanthone: resistance of schistosoma mansoni to schistosomicidal agents.fourteen patients with active schistosomiasis mansoni in spite of previous treatment with oxamniquine and/or hycanthone were treated with praziquantel, single oral dose of 45 to 50 mg/kg body-weight. all underwent clinical, laboratory and electrocardiographic examination before and after treatment. untoward effects (dizziness, drowziness, nausea and abdominal pain) were observed in ten. laboratory findings disclosed no significant alteration and the electrocardiograms showed no abnormalities. mo ...19827179419
[comparative study of the susceptibility of biomphalaria glabrata populations of belo horizonte (mg), to schistosoma mansoni infection and to molluscacides]. 19827182912
[study of the mechanisms of rejection in incompatible mollusk-schistosome pairs from infestations by means of a natural route or by microsurgical transplantations of parasitic stages].the non-compatible host-parasite combination used in these studies is an albino brazilian strain of biomphalaria glabrata (bre) and a guadeloupean strain of schistosoma mansoni (gua). this work allows us to emphasize three main points: --the failure of infestation by miracidia transplantations in the non-compatible combination indicates that the origin of resistance does not lie at the level of the tegument, but further away, in the hemolymph of the host. --the results of the histological studie ...19826131592
schistosoma mansoni: quantification of skin penetration and early migration by differential external radioassay and autoradiography.eleven gamma-emitting radionuclides (49sc, 54mn, 59fe (+2 and +3), 60co, 65zn, 75se (as selenomethionine, selenocystine, selenite and selenate), 109cd, 125sb, 133ba, 137cs and 203hg) were screened as labelling agents for schistosoma mansoni cercariae by incubation of infected biomphalaria glabrata snails in radioactive solution according to the technique of christensen (1977). only [75se]methionine yielded satisfactorily labelled cercariae. multiple regression analysis of volume, number of cerca ...19826803218
effect of schistosoma mansoni on glycogen synthase and phosphorylase from biomphalaria glabrata (mollusca). 19826804614
elimination of biomphalaria pfeifferi, bulinus tropicus and lymnaea natalensis by the ampullarid snail, marisa cornuarietis, in a man-made dam in northern tanzania.marisa cornuarietis is a well known ampullarid competitor/predator of biomphalaria glabrata in puerto rico. for the first time in africa a flourishing population of marisa has been established in a small, permanent, man-made dam at kisangara, near moshi, tanzania. prior to the release of m. cornuarietis in june 1977, this dam supported thriving populations of the pulmonate snail hosts biomphalaria pfeifferi and lymnaea natalensis; bulinus tropicus and the melaniid melanoides tuberculata were als ...19826122367
introduction of biomphalaria glabrata to egypt and other african countries. 19826926781
altered feeding response as a cause for the altered heartbeat rate and locomotor activity of schistosoma mansoni-infected biomphalaria glabrata.biomphalaria glabrata infected with schistosoma mansoni for 33 days fed more often than uninfected snails. whereas uninfected snails had nocturnal increases in feeding, snails with a 33-day-old infection of s. mansoni fed as often during the day as in the night. using direct observation and film analysis, we found that feeding increased the heartbeat rate and locomotor activity of b. glabrata. when snails were allowed to feed ad lib., infected snails had higher heartbeat rates than uninfected sn ...19836631636
effects of alterations in the heartbeat rate and locomotor activity of schistosoma mansoni-infected biomphalaria glabrata on cercarial emergence.during a 27-hr period of food deprivation, biomphalaria glabrata infected with schistosoma mansoni had lower heartbeat rates and locomotor activities than did controls that were feeding ad lib. however, there was no difference between the cercarial emergence from control and experimental snails either before, during, or after the period of food deprivation. no correlation was found between the locomotor activity of the snail and the number of cercariae emerging from the snail in continuous light ...19836631637
purine metabolism in biomphalaria glabrata under starvation and infection with schistosoma mansoni.purine content and its alterations during a starvation period of 29 days and an infection period of 52 days were studied. during starvation the amount of uric acid increased from 2.11 +/- 0.95 mg/g dry wt in control snails to a maximum of 6.73 +/- 3.99 mg/g dry wt on day 29 of starvation for uric acid and from 4.73 +/- 2.10 mg/g dry wt in control snails to a maximum of 11.29 +/- 3.48 mg/g dry wt on day 23 of starvation for guanine. during infection there was an increase with both uric acid and g ...19836641159
[experimental infection of biomphalaria glabrata with schistosoma mansoni by the injection of miracidia]. 19836672864
lysosomal enzyme activities in susceptible and refractory strains of biomphalaria glabrata during the course of infection with schistosoma mansoni.the distribution and abundance of the lysosomal enzyme markers, acid phosphatase (ap), peroxidase (po), and nonspecific esterase (ne), within circulating blood cells (hemocytes) were examined in a schistosome-susceptible (pr albino m-line) and a resistant (10-r2) strain of biomphalaria glabrata during the course of infection with schistosoma mansoni. the dynamics of serum (cell-free hemolymph) ap activities and total hemocyte numbers in infected snails also were investigated. hemocyte subpopulat ...19836674450
schistosoma mansoni: immunofluorescent detection of its antigen reacting with biomphalaria glabrata amoebocytes.the reaction of amoebocytes in the hemolymph of the infected intermediate host, biomphalaria glabrata, to schistosoma mansoni antigens has been investigated using the indirect immunofluorescence antibody test. monolayers of amoebocytes, prepared from hemolymph of infected and normal snails, were first fixed and then reacted with antisera obtained from mice infected for 7 to 9 weeks. nonspecific and cross-reactions between the antisera and monolayers of amoebocytes were eliminated by absorbing th ...19836337058
isolation and characterization of a lectin from the snail biomphalaria glabrata and a study of its combining site.the hemagglutinins from the spawn of the water snail biomphalaria glabrata were isolated by affinity chromatography on hog gastric mucin coupled to sepharose 4b. the n-acetyl-d-glucosamine eluate (0.1 m) was fractionated further on bio-gel p-300, yielding two fractions. fraction 1 had an mr of 350 000 and displayed one band in immunoelectrophoresis, but was heterogeneous in discontinuous electrophoresis. it agglutinated human red blood cells with a1 and b specificity at concentrations of 12 and ...19836360215
laboratory comparison of the relative success of biomphalaria glabrata stocks which are susceptible and insusceptible to infection with schistosoma mansoni.a method of interrupting the life-cycle of the human blood fluke schistosoma by increasing the proportion of genetically insusceptible intermediate host snails in natural populations was first proposed nearly 25 years ago. the method assumes that insusceptible snails will be at a selective advantage over susceptible snails when the schistosome parasite is present, and therefore natural selection will act to increase the proportion of alleles for insusceptibility. a major objection to the propose ...19836856336
acquired resistance in snails. induction of resistance to schistosoma mansoni in biomphalaria glabrata. 19836874229
displacement of biomphalaria glabrata by the snail thiara granifera in field habitats in st. lucia, west indies.thiara granifera is a melaniid snail capable of maintaining very high densities in a variety of habitats. it has been introduced into the new world from the far east and is now spreading rapidly throughout the caribbean. in puerto rico and dominica casual observations following natural invasion by t. granifera suggest that it may exert a powerful restraining influence on populations of biomphalaria glabrata, the major intermediate host of schistosoma mansoni in the caribbean. the potential of t. ...19836882056
mimicry of snail host antigens by miracidia and primary sporocysts of schistosoma mansoni.polyvalent antisera generated in rabbits to soluble haemolymph components from schistosoma mansoni-susceptible (pr albino 'm line') and s. mansoni-resistant (10-r2) stocks of the snail biomphalaria glabrata were employed as membrane probes to determine if antigens related to snail haemolymph were produced by the early larval stages of s. mansoni (pr-1 strain). using immunofluorescence and immunoelectron microscopical methods we have demonstrated that antibodies to susceptible (anti-suscept) and ...19836191268
schistosoma mansoni and biomphalaria glabrata share epitopes: antibodies to sporocysts bind host snail hemocytes. 19836195272
surface antigens of biomphalaria glabrata (gastropoda) hemocytes: occurrence of membrane-associated hemolymph-like factors antigenically related to snail hemoglobin. 19836190950
the ultrastructure of the amebocyte-producing organ in biomphalaria glabrata.the amebocyte-producing organ (apo) in normal and echinostome-sensitized biomphalaria glabrata was studied at the ultrastructural level. the apo in unexposed snails consists of small clusters of primary ameboblasts resting on the epithelial cells lining the pericardium. the ameboblasts are held in a loose reticulum formed by extensions from smooth muscle and few fibroblastic cells. secondary ameboblasts and amebocytes constitute further stages of this cell line. amebocytes, resembling cells in t ...19836683673
[microflora and microfauna of the habitat of biomphalaria glabrata (say, 1818)]. 19836680234
identification of antigenically distinct hemocyte subpopulations in biomphalaria glabrata (gastropoda) using monoclonal antibodies to surface membrane markers.five monoclonal antibodies (mabs) against surface antigens on circulating, glass-adherent hemocytes of the snail, biomphalaria glabrata, were produced by somatic cell hybridization methods. two mabs (iid2.6-bg and iid4.8-bg) are pan-hemocytic, reacting uniformly with epitopes shared by all adherent hemocytes. determinants recognized by these mabs also are present in soluble form and appear to be associated with a hemoglobin-depleted ultracentrifuged fraction of snail hemolymph. hybridoma-derived ...19836192927
on the molluscicidal activity of tannin containing plants.the aqueous and methanolic extracts of a series of typical tannin containing plants exhibit strong molluscicidal properties against the freshwater snail biomphalaria glabrata, which is the intermediate host of schistosomiasis. the crude extracts of krameria triandra (krameriaceae) and arctostaphylos uvaursi (ericaceae) were active at concentrations of 50 ppm. hydrolyzable and condensed tannins are responsable for the molluscicidal activity. this report indicates a new class of plant derived comp ...198317404963
[effect of metallic magnesium and various magnesium salts on the oviposition of biomphalaria glabrata (say, 1818)]. 19836689541
[biological control: helobdella triserialis lineata blanchard, 1849 (hirudinea: glossiphonidae) on biomphalaria glabrata say, 1818 (mollusca: planorbidae), in the laboratory]. 19836675164
specificity of ovulation hormones of some basommatophoran species studied by means of iso- and heterospecific injections.isospecific injections of intercerebral commissure (com) extracts of l. stagnalis, l. palustris, and l. ovata (lymnaeidae) and of extracts of com/mediodorsal bodies complexes (com/db) of bulinus truncatus (bulinidae) and biomphalaria glabrata (planorbidae) induced oviposition in a high percentage of the experimental snails. positive results of heterospecific injections between the first three species indicated a likely structural similarity of the ovulation hormones within the genus lymnaea. the ...19836685080
chronic exposure of the eggs and adults of biomphalaria glabrata (say) to the molluscicide, nicotinanilide.some effects of chronic, low-dosage treatment of biomphalaria glabrata eggs and adults with nicotinanilide, are reported. the susceptibility of eggs decreased with age. the development and hatching of 0-1 day old eggs could be prevented by continuous exposure to 0.0005-0.001 mg/l-1 whereas prehatch stages had a susceptibility similar to that of adult snails (0.1-0.2 mg/l-1). the possible use of slow-release formulations of nicotinanilide in the control of field populations of snails and schistos ...19836636300
surface antigens of biomphalaria glabrata (gastropoda) hemocytes: evidence for linkage-independence of some hemolymph-like surface antigens and con a receptor-bearing macromolecules. 19836886469
genetic selection for tolerance to niclosamide and copper in biomphalara glabrata (mollusca: pulmonata).successive generations of the pr-79 and m-line laboratory stocks of biomphalaria glabrata were exposed in the lc90 of niclosamide or copper sulfate. survivors from each generation produced the succeeding generation by self fertilization. the pr-79 stock showed no evidence of tolerance to either molluscicide following five generations of selection. on the other hand, m-line f5 snails demonstrated approximately two-fold higher tolerance to both molluscicides than did non-selected m-line snails.19846495387
an ultrastructural study on ventricular encapsulation reactions in biomphalaria glabrata exposed to irradiated echinostome parasites. 19846735576
equations for describing growth of the schistosome host snail biomphalaria glabrata.biomphalaria glabrata and stagnicola palustris were raised in aquaria under crowded and uncrowded conditions. measurements of greatest shell diameter were taken at regular intervals. von bertalanffy and logistic growth curves were fitted by least squares and maximum likelihood methods. the resulting parameter estimates produced better fits for the logistic equation than for the von bertalanffy equation.19846737172
[in vitro development of hemolymph granulocytes of biomphalaria glabrata (gastropoda, planorbidae). study by scanning electron microscopy].the morphological evolution in vitro of biomphalaria glabrata granulocytes on a cover-glass is described by scanning electron microscopy. 4 stages have been characterized: stage 1: globular cells showing low adhesion to cover-glass; stage 2: flattened cells in contact with substrate over a large surface, which present two well differentiated cytoplasmic zones: an endoplasm in which the nucleus is embedded and an ectoplasm which extends into very thin filopodia with a spherical terminal bulb; sta ...19846508142
immune-recognition of schistosoma mansoni primary sporocysts may require specific receptors on biomphalaria glabrata hemocytes.cellular interactions, leading to cell-mediated cytotoxicity when biomphalaria glabrata hemocytes encapsulate schistosoma mansoni sporocysts, have been investigated. rabbit antibodies (igg), when bound to antigens on sporocyst surfaces, prevent the normal cytoadherence (ca) of hemocytes from both susceptible and resistant host snails. since interference with ca occurs with even fixed sporocysts, the effect is not due to igg stimulated modulation of the parasite surface. using two antisera with s ...19846240624
[selection of genotypes of schistosoma mansoni and their maintenance by sporocyst transplantation].schistosoma mansoni was isolated by hatching eggs obtained from a naturally infected rat in grand etang, guadeloupe; fifty biomphalaria glabrata were exposed to five miracidia each. the resulting cercariae were used to infect laboratory mice which were later sacrificed to provide worms for enzyme analyses and eggs for further infections. seven enzymes in extracts of individual worms were examined by isoelectric focusing in polyacrylamide gels: acp, g6pdh, pgm, gpi and hk showed no variation, whe ...19846437612
spirulina as a nutrient source in experimental media for maintaining the schistosome vector, biomphalaria glabrata. 19846441531
an ultrastructural study of the effect of parasitism by larval schistosoma mansoni on the calcium reserves of the host, biomphalaria glabrata.the tissues of biomphalaria glabrata contain three types of calcium cells which can be differentiated by their location and the size and number of their contained corpuscles. x-ray analysis has confirmed the presence of calcium and magnesium in the corpuscles. molluscs containing the larval stages of schistosoma mansoni at 40 days post infection show disintegration of the calcareous corpuscles in type-a calcium cells and erosion of the inner surface of the shell.19846467339
experimental studies of age-prevalence curves for schistosoma mansoni infections in populations of biomphalaria glabrata.we report the results of experimental studies of the generation of age-prevalence curves for schistosoma mansoni infections in laboratory populations of biomphalaria glabrata. within snail populations of varying sizes and age structures, the net force of infection is shown to be linearly dependent on the rate at which miracidia are introduced into the aquatic habitat of the host. for individual snails, the per capita force of infection is shown to be related to snail age and size, and the death ...19846472887
schistosoma mansoni: passive transfer of resistance by serum in the vector snail, biomphalaria glabrata.passive transfer of natural resistance to schistosoma mansoni (pr-1 strain) has been successfully accomplished in the snail intermediate host, biomphalaria glabrata (pr albino, m-line strain). injection of serum (cell-free hemolymph) from a naturally schistosome-resistant strain of b. glabrata (10-r2) into pr albino snails induced a complete protection from a primary infection with the parasite in 29 of 48 snails (60.4%). in comparison, inoculation of homologous pr albino serum or heterologous p ...19846479289
role of cellular and humoral components in the encapsulation response of biomphalaria glabrata to schistosoma mansoni sporocysts in vitro. 19846483876
isolation, characterization and functional assessment of a hemagglutinin from the plasma of biomphalaria glabrata, intermediate host of schistosoma mansoni.an agglutinin from the plasma of biomphalaria glabrata, vector of schistosoma mansoni, has been isolated and partially characterized. its isolation is a simple two-step process, yielding an essentially pure preparation that can then be manipulated free from other plasma components. it is a large glycoprotein (10(6) daltons), found in four of the five snail strains tested, and possesses affinity for galactose-type sugar residues. based upon criteria such as molecular weight, carbohydrate and eryt ...19846500135
chronobiological aspects of the host-parasite relationships between biomphalaria glabrata and schistosoma mansoni: cercarial production and infectivity, and growth kinetics of the host. 19846501917
intergeneric reproductive stimulation and parthenogenesis in schistosoma mansoni.when mice were infected with female cercariae of schistosoma mansoni and male cercariae of schistosomatium douthitti, many mixed pairs formed. the paired females were approximately the same size as those in unisexual infections, far smaller than females paired with s. mansoni males. although the sch. douthitti males possessed well-developed testes, sperm were not found in their female partners, which developed scanty vitelline glands and produced laterally spined eggs typical of s. mansoni. such ...19846504564
is planorbarius metidjensis compatible with schistosoma haematobium and s. bovis?on the basis of laboratory snail infection experiments it is concluded that planorbarius metidjensis is unimportant in the epidemiology of schistosoma haematobium in north west africa. 410 p. metidjensis spain were exposed to 8 isolates of s. haematobium from ghana, kenya, madagascar, mauritius, morocco, tanzania, south africa and sudan. 50 p. metidjensis morocco and 50 p. metidjensis algarve were exposed to s. haematobium morocco. all infections failed to develop to maturity. serial sections of ...19846509700
[activities of a schistosoma mansoni focus of transmission in martinique (french antilles)].a focus of intestinal schistosomiasis was discovered in november 1981 in martinique in the saint-pierre area. an analysis of its structure and of the mecanisms of the transmission of the parasite was carried out between 1981 and 1983. this focus presents the following particularities: a transmission site very small constituted by water cress beds; a small human population infected with a prevalence of 13% (positive stools) to 41,3% (positive serologies); a weak fecal contamination of the water; ...19846524823
the adenylate nucleotide pool in the digestive gland-gonad complex of biomphalaria glabrata infected by schistosoma mansoni.the levels of adenylate nucleotides were examined in the digestive gland and ovotestes of biomphalaria glabrata during cercarial shedding of schistosoma mansoni, 10 weeks post-infection. in general, parasitization resulted in decreases in the adenylate levels in both tissues, but the results were not statistically significant. moreover, the energy charge ratio was not significantly altered. the mean energy charge in ovotestes from uninfected and infected individuals was 0.81 and 0.77, respective ...19846527694
[biomphalaria glabrata in the state of piaui].the occurrence of biomphalaria glabrata is recorded for the first time in the state of piaui, where it was collected from several breeding places in the city of parnaíba. examination of 694 specimens showed that a part of them were infected with trematodes other than schistosomatidae. so far no autochthonous cases of schistosomiasis have been identified in the city. the presence of b. glabrata in parnaíba extends by 20 km eastward its range on the northern coastal region of the great northeaster ...19846535921
[new foci of transmission of schistosoma mansoni in the state of pará].two new foci of transmission of schistosoma mansoni in the state of pará are recorded, with the finding of naturally infected biomphalaria glabrata in the municipalities of viseu and belém. uninfected specimens of biomphalaria straminea, as well as the planorbid species biomphalaria schrammi, drepanotrema lucidum and d. anatinum, were found in the same area.19846535922
comparison of the rhythmic emergence of schistosoma mansoni cercariae from biomphalaria glabrata in different lighting regimens. 19846541689
[chemoprophylaxis of schistosomiasis: molluscacidal activity of natural products--assays with adult snails and oviposition].data concerning the molluscicidal activity of 159 extracts from 84 brazilian plants on biomphalaria glabrata, the most important intermediate host of schistosoma mansoni in brazil, are presented. seventy eight extracts (49,0%) showed activity against snails or eggs, but only twenty nine (18.2%) were active on both, snails and eggs. extracts of two species (mikania hirsutissima and qualea multiflora) have shown to be lethals to adult snails at 10 ppm concentration.19846548884
schistosoma mansoni: long-term maintenance of clones by microsurgical transplantation of sporocysts.schistosoma mansoni sporocysts originally derived from monomiracidially infected biomphalaria glabrata snails were serially transplanted into the cephalopedal sinus of anesthetized snails by the microsurgical implantation of fragments of parasitized hepatopancreas and ovotestis. three to six passages each of five male and five female clones were maintained for as long as 2.0 years. of the recipient snails which survived surgery, 87% released cercariae, usually beginning 5-7 weeks after surgery. ...19846692880
[experimental schistosomiasis iii. observations on the infestation of biomphalaria glabrata by a miracidium of schistosoma mansoni].380 b. glabrata infected with 1 miracidium of s. mansoni produced 87 positive snails (22,9%), 38 of which sending out male cercariae and 49 female cercariae. the snails belonged to 3 experimental series kept at 27 degrees and 24 degrees c for 15 weeks under continuous lighting. there was no difference between the proportions of both sexes. the survival of the positive snails was favoured by the male sex. the shedding of female cercariae was greater than shedding of male. during those 15 weeks, t ...19846721371
effects of temperature on the larval development of angiostrongylus cantonensis in the intermediate host, biomphalaria glabrata.the effects of temperature on the larval development of angiostrongylus cantonensis in biomphalaria glabrata were studied under controlled conditions. when b. glabrata were maintained at different, constant temperatures, the first-stage larvae developed to third-stage larvae between 20 degrees and 31 degrees c. the velocity of development to the third-stage in the snail depended on the relationship, y = -0.1281 + 0.0081 x (y velocity of development; x rearing temperature). the "threshold of deve ...19846741223
schistosoma mansoni: agglutination of sporocysts, and formation of gels on miracidia transforming in plasma of biomphalaria glabrata.the resistance or susceptibility of biomphalaria glabrata strains to strains of schistosoma mansoni, the human blood fluke, are evidenced by the responses of snail hemocytes to sporocysts of the schistosome, both in vivo and in vitro. it is now reported that living sporocysts of the pr1 strain of s. mansoni agglutinate in the plasma of all tested strains of b. glabrata, in contrast to fixed sporocysts which agglutinate only in plasma from resistant snail strains. the agglutinating activity in re ...19846745386
isolation and partial characterization of an antigen shared between schistosoma mansoni, fasciola hepatica, and biomphalaria glabrata.an antigen was isolated and partially characterized from adult schistosoma mansoni which immunologically cross-reacted with fasciola hepatica and biomphalaria glabrata, a schistosome intermediate snail host. the antigen was isolated from a solubilized freeze-thaw preparation containing 0.5% triton x-100 by passage through a cnbr-activated sepharose 4b column coupled with rabbit igg prepared against a homogenate of b. glabrata hepatopancreas. the eluted antigen (designated as smw-53p) stained wit ...19852419536
enzyme histochemical comparison of biomphalaria glabrata amebocytes with human granuloma macrophages.in fresh water snails, amebocytes are the principal cells that react to parasitic infection. ultrastructurally, amebocytes resemble mammalian macrophages. to clarify the relationship between amebocytes and macrophages, we compared the histochemical staining for seven enzymes in biomphalaria glabrata snail amebocytes, both in the amebocyte-producing organ (apo) and in the encapsulation reaction formed around parasite sporocysts with the staining in macrophages from the lymph nodes of patients wit ...19852981947
molluscicide activity of the "avelós" plant (euphorbia tirucalli, l.) on biomphalaria glabrata, the mollusc vector of schistosomiasis.an aqueous solution of the latex of euphorbia tirucalli collected at sites receiving large amounts of sunlight showed molluscicide action on biomphalaria glabrata, with ld50 obtained at the concentration of 28,0 ppm and ld90 at the concentration of 85,0 ppm. the toxicity of the product for fish was similar to that of bayluscide and of copper sulfate used for comparison. however, the wide distribution of the plant, its easy propagation and the simple procedure for extraction of the active substan ...19853939311
genetics of biomphalaria glabrata: linkage analysis of genes for pigmentation, enzymes, and resistance to schistosoma mansoni.the snail, biomphalaria glabrata, is a major intermediate host of the human blood fluke, schistosoma mansoni, in the americas. the inheritance and linkage relationships of a gene enabling adult snails to resist infection by a puerto rican strain of the parasite were analyzed using two laboratory stocks that differed in susceptibility, pigmentation, and five electrophoretically detectable enzyme markers. segregation ratios in second-generation intraspecific hybrids between susceptible (m-stock) a ...19853866575
effect of high dosages of bacterial challenge on acid phosphatase release from biomphalaria glabrata hemocytes. 19853920329
release pattern of aminopeptidase from biomphalaria glabrata hemocytes subjected to high-level bacterial challenge. 19853923126
assessment of schistosomiasis in the dominican republic.active transmission of intestinal schistosomiasis is currently limited to the southeastern part of the dominican republic. a population-based stool survey in 1980 detected 4 asymptomatic individuals among 114 selected at random in 2 towns and a rural community in el seibo province. the distribution of the transmitting snail, biomphalaria glabrata, considerably exceeds that of schistosoma mansoni, extending to the national district and capital city of santo domingo and well into certain central v ...19853931167
isolation and partial characterization of shared antigens of biomphalaria glabrata and schistosoma mansoni and their evaluation by the elisa and the eitb.serum from humans infected with schistosoma mansoni, when reacted with a biomphalaria glabrata soluble hepatopancreas antigen extract (bgsha) yields 2 lines of precipitation by gel diffusion and 1 by immunoelectrophoresis. the igg from the serum of a human infected with s. mansoni was coupled to cnbr-activated sepharose 4b. bgsha (8.0 mg) was then filtered through the gel and the bound antigens, denoted bgsm, eluted with hcl-glycine, ph 2.6. these bound antigens comprised 2.8% of the total bgsha ...19853932633
pathophysiologic alterations in biomphalaria glabrata infected with angiostrongylus costaricensis. 19853981029
biomphalaria glabrata (gastropoda): effect of urethane on the morphology and function of hemocytes, and on susceptibility to schistosoma mansoni (trematoda). 19853998486
[factors responsible for preventing the carrying out of the schistosoma mansoni cycle in the waters of grande terre of guadeloupe (french antilles)].different surveys which have been conducted in ponds and pools in the grande terre of guadalupe, have demonstrated the absence of schistosoma mansoni transmission in spite of apparent favourable ecological conditions. four hypothesises can explain this absence of transmission: 1 - the presence of biomphalaria glabrata populations non-susceptible to the infestation by s. mansoni. 2 - ecological conditions unfavourable to the survival of parasitized snails. 3 - ecological conditions unfavourable t ...19854004031
schistosoma mansoni: lectin-dependent cytotoxicity of hemocytes from susceptible host snails, biomphalaria glabrata.normally benign hemocytes from a strain (m-line) of the snail, biomphalaria glabrata, susceptible to schistosoma mansoni, became cytotoxic toward the sporocyst stage if the parasite was first treated with the lectin, concanavalin a. concanavalin a binding was inhibitable with alpha-methyl mannoside and killing was dose-dependent. maximal levels of concanavalin a-induced cytotoxicity were comparable with levels observed when hemocytes from a resistant snail strain (13-16-r1) encountered untreated ...19854040473
plasma components which mediate cellular defences in the gastropod mollusc biomphalaria glabrata.blood plasmas from certain strains of biomphalaria glabrata are known to have components which facilitate a hemocyte-effected cytotoxic response against encapsulated schistosoma mansoni sporocysts. the possible identity of the factor(s) has been investigated. sporocysts placed in snail plasma rapidly acquire a wide variety of host plasma antigens, at least some of which are displayed on the parasite surface. plasmas from strains of snail resistant to the parasite agglutinate fixed sporocysts, wh ...19854043487
31p nmr studies on adenylates and other phosphorus metabolites in the schistosome vector biomphalaria glabrata.31p nuclear magnetic resonance spectroscopy was applied successfully to whole intact and de-shelled snails and to the isolated digestive gland-gonad tissue complex as well as other tissues of biomphalaria glabrata. several phosphorus metabolites, including atp and adp, were observed. the mean atp/adp ratio calculated for the tissue complex was 3.1 and the atp concentration was 0.73 nmoles/mg tissue fresh weight. assignments for amp, sugar phosphates, and inorganic phosphate peaks were tentativel ...19854057009
biomphalaria glabrata in haiti.biomphalaria glabrata, an intermediate host for schistosoma mansoni, was first reported in haiti in 1891 at one location in the départment du nord and in 1977 it was reported at several sites in one additional watershed. our study identifies two additional locations each on a different watershed plus a third possible site. a wide but discontinuous distribution of the snail on the north coast of haiti is confirmed (no autochthonous infections with s. mansoni have been reported). while there are m ...19854082255
orientation to light of juvenile and adult forms of melanic and albino populations of biomphalaria glabrata(say, 1818). 19854088040
[resistance of biomphalaria schrammi of arcos, minas gerais, brazil, to infection with 2 strains of schistosoma mansoni].the descendants of planorbid snail biomphalaria schrammi crosse, 1864, collected in the region of arcos, state of minas gerais, brazil, were exposed to miracidia of two strains of schistosoma mansoni: the "le" strain from belo horizonte, minas gerais state and the "sj" strain from são josé dos campos, state of são paulo. of the 172 snails exposed to miracidia of both strains, none were infected. on the other hand, 100 biomphalaria glabrata snails of the control group showed infection rates of 88 ...19854088045
[a stock of infected snails for the mass breeding of cercariae and schistosomules].a routine to maintain infected snails biomphalaria glabrata able to produce 1-2 million cercariae per week and a mean shedding of about 3,500 cercariae per snail was quantitatively described. to a monthly production of about 5.3 and 6.5 million cercariae during the years of 1982 and 1983 it was necessary to expose an average of 1,557 and 1,957 snails, respectively. the efficiency described was maintained by infecting 2,000 snails per month (infectivity index of about 60%). a maximal production o ...19854088046
surface antigens of biomphalaria glabrata (gastropoda) hemocytes: functional heterogeneity in cell subpopulations recognized by a monoclonal antibody. 19853981030
[effect of stevia rebaudiana in biomphalaria glabrata]. 19863575816
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