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beta-glucan hydrolases from aspergillus niger. isolation of a beta-(1-4)-glucan hydrolase and some properties of the beta-(1-3)-glucan-hydrolase components.1. the components of an enzyme preparation from aspergillus niger, which hydrolysed substrates containing beta-(1-->3)- and beta-(1-->4)-glucosidic linkages, were separated by calcium phosphate and dowex 1 column chromatography. 2. the hydrolytic activity of each fraction from both types of column towards laminaribiose, laminarin, carboxymethylpachyman, pachydextrins, salicin, cellobiose, cellopentaose and swollen cellulose was tested. 3. the activity towards the beta-(1-->3)-glucosidic substrat ...19655862417
properties of a beta-(1-4)-glucan hydrolase from aspergillus niger.1. a beta-(1-->4)-glucan hydrolase prepared from aspergillus niger, as described by clarke & stone (1965a), showed a ph optimum in the range 4.5-6 and k(m) 0.25% when acting on a cellulose dextrin sulphate substrate. 2. the hydrolase rapidly decreased the specific viscosity of carboxymethylcellulose with a small increase in the production of reducing sugars. the identity of the products of hydrolysis of cellotetraose, cellopentaose and their reduced analogues indicate a preferential cleavage of ...19655862418
production of the enzyme naringinase by aspergillus niger.the formation of naringinase, a glycolytic enzyme produced by aspergillus niger, is repressed by glucose. production of the enzyme is decreased below ph 4.0 and is stimulated by the presence of substrate. fermentation conditions are described which cause the formation of the enzyme in approximately a fivefold greater concentration than that previously described.19655866035
[malate dehydrogenase and glyoxylate reductase of aspergillus niger]. 19655873329
[on the knowledge of the so-called glucose oxidase. 8. effect of a variation of c and n sources on the induced enzyme formation by aspergillus niger]. 19655884705
[on the methods of selecting mutants of aspergillus niger with altered capacity to synthetize organic acids]. 19655887708
[investigations on the effect of amino acids on the formation of citric acid by aspergillus niger. 1. action of several organic nitrogen sources on the mycelium activity of aspergillus niger in surface culture]. 19655898369
microbiological transformations of terpenes. v. origin of 2-nonene, 2,3-dicarboxylic acid anhydride in the fermentation of terpenoid hydrocarbons by aspergillus niger. 19654221006
purification and properties of carboxymethyl cellulase from aspergillus niger. 19654158106
studies on citric acid synthesis. 3. the synthesis of vitamins b by strains of aspergillus niger of different acid production activity. 19654159117
report on a case of subcutaneous dissemination of aspergillus niger, type awamori. 19654162961
studies on citric acid synthesis. ii. the amino acid composition of cultures of aspergillus niger of varying acid production ability. 196514338866
enzymic synthesis of o-beta-d-glucopyranosyl-(1--6)-d-galactose.1. the enzymic synthesis of o-beta-d-glucopyranosyl-(1-->6)-d-galactose has been described and evidence for the structure presented. 2. it has been shown that the transglycosylase of a. niger provides a convenient means of synthesizing (1-->6)-linked disaccharides.196514340044
[on data on so-called glucose oxidase. vii. effect of quantitative variations of a basic medium on induced glucose oxidase formation by aspergillus niger]. 196514296686
purification and properties of the glucose oxidase from aspergillus niger. 196514299649
fungal detoxication. vii. metabolism of 2,4-dichlorophenoxyacetic and 4-chloro-2-methylphenoxyacetic acids by aspergillus niger. 196514267231
production of the enzyme naringinase by aspergillus niger.[this corrects the article on p. 842 in vol. 13.].196616349665
nonessentiality of boron in fungi and the nature of its toxicity.an investigation was undertaken to determine whether any of the following fungi had a requirement for boron (b): saccharomyces cerevisiae, aspergillus niger, neurospora crassa, and penicillium chrysogenum. boron was unessential, and hence a study was made of the concentrations of b that reduced the growth of s. cerevisiae and p. chrysogenum and the mode of action of the b toxicity. fifty and 4000 mg b/liter, respectively, significantly (5% level) reduced the growth of the latter 2 species.in bot ...196616656256
[demonstration of a cytochrome c-peroxidase activity in aspergillus niger]. 19664225076
studies on nutritional requirement, chemical composition & enzyme make-up of aspergillus niger cultivated in medium containing d-tartaric acid as the sole carbon source. 19664225710
comparison of chemical changes & enzyme make-up of wild strain, & citrate & tartrate adapted strains of aspergillus niger. 19664225712
enzymic hydrolysis of barley and other beta-glucans by a beta-(1--4)-glucan hydrolase.1. a barley glucan with 68% of beta-(1-->4)-linkages and 32% of beta-(1-->3)-linkages was exhaustively hydrolysed with an aspergillus niger beta-(1-->4)-glucan 4-glucanohydrolase (ec 3.2.1.4) (clarke & stone, 1965b). the hydrolysis products were separated and estimated. 2. the lower-molecular-weight products were identified as: glucose, 1.4%; cellobiose, 11.9%; 3(2)-o-beta-glucosylcellobiose, 45.0%; a tetrasaccharide(s), which was a substituted cellobiose, 16.4%. a series of unidentified higher- ...19664290404
[malate dehydrogenase of aspergillus niger]. 19664296592
inhibitory effect of the vapours of antifungal compounds on aspergillus niger. 19665912078
the effect of substituted phenoxyacetic and phenoxybutyric acids on the growth and respiration of aspergillus niger. 19665912592
on the reaction of the glucose oxidase from aspergillus niger with bisulfite. 19665913131
direct evidence for the conversion of dethiobiotin to biotin in aspergillus niger. 19665928209
electromicroscopy studies of conidial walls of sphaerotheca macularis, penicillium levitum, and aspergillus niger. 19665929166
the effect of hydrogen peroxide on glucose oxidase from aspergillus niger. 19665938931
synthesis of malformin by an enzyme preparation from aspergillus niger.yukioka, m. (university of hawaii, honolulu), and t. winnick. synthesis of malformin by an enzyme preparation from aspergillus niger. j. bacteriol. 91:2237-2244. 1966.-an enzyme fraction derived from disrupted aspergillus cells was able to utilize each of the component labeled amino acids of malformin for the synthesis of this cyclic pentapeptide. the process was stimulated by adenosine triphosphate, k(+), and mg(++), and was optimal at approximately ph 8.5. it was not affected by inhibitors of ...19665943939
fungal detoxication. 8. metabolism of substituted 4-phenoxybutyric acids by aspergillus niger. 19665948687
aspergillus niger infection of the eye. 19665957897
formation of protoplasts in aspergillus niger. 19665958594
[studies on the cytochemical determination of glucose oxidase (e.c. 1.1.3.4) in aspergillus niger]. 19665959123
biosynthesis of malformin in washed cells of aspergillus niger. 19665963030
the pectic enzymes of aspergillus niger. a mercury-activated exopolygalacturonase.1. an exopolygalacturonase was separated from a mycelial extract of aspergillus niger with a 290-fold purification and a recovery of 8.6%. 2. the enzyme displayed its full activity only in the presence of hg(2+) ions; k(a) for mercuric chloride was about 6x10(-8)m. 3. the mercury-activated enzyme progressively removed the terminal galacturonic acid residues from alpha-(1-->4)-linked galacturonide chains and converted digalacturonic acid, trigalacturonic acid, tetragalacturonic acid and pectic ac ...19665964957
aconitase and isocitric dehydrogenases of aspergillus niger in relation to citric acid production. 19665965361
effects of d2o on citric acid and pyruvate carboxylase formation by aspergillus niger. 19665966515
effect of ferrocyanide on growth and citric acid production by aspergillus niger. 19665972643
proteinase formation in relation to physiological activities of growing mycelia of aspergillus niger. 19665982534
[studies of acid metabolism of aspergillus niger. ii. the effect of c-n ratio in the original medium on the fluconic, citric and oxalic acid accumulation at controlled ph value and medium temperature (30 degrees c)]. 19665985352
[on theeffect of hydrogen peroxide on citric acid accumulation in aspergillus niger]. 19665989268
[studies on the metabolism of acids in aspergillus niger. iv. on the participation of autolysis in the accumulation of xalic acid]. 19665989423
[studies on the metabolism of acids in aspergillus niger. 3. effect of the c/n ratio in the starting culture medium on the accumulation of gluconic-, citric-, and oxalic acid under controlled ph and extreme temperatures (43 and 10 deree c)]. 19665989526
[studies on the metabolism of acids in aspergillus niger. v. on the participation of the catabolism of gluconic and citric acid in the accumulation of oxalic acid]. 19665992183
[aspergillus niger alanine dehydrogenase]. 19666002774
[aspergillus niger variability provoked by combined effect of ethylenimine and uv-irradiation]. 19666002783
[toxicity of lead to aspergillus niger]. 19666003006
effects of glucose oxidase inhibition on carbohydrate metabolism in aspergillus niger. 19666005321
biosynthesis of citric acid. 3. dynamic role of metal ions in the production of citric acid by aspergillus niger. 19666013721
micromorphology of conidiospores of aspergillus niger by electron microscopy. 19665298079
the influence of metal-complexing agents on citric acid production by aspergillus niger. 19664959280
the pectic enzymes of aspergillus niger. a second exopolygalacturonase.1. a second exopolygalacturonase was separated from a mycelial extract of aspergillus niger with a 265-fold purification and a recovery of 1%. 2. unlike the first exopolygalacturonase this enzyme showed no requirement for metal activators, nor was it inhibited by chelating agents. 3. the two exopolygalacturonases were also distinguished by their ph optima and stability. 4. the enzyme progressively removed the terminal galacturonic residues from alpha-(1-->4)-linked galacturonide chains, converti ...19664960793
[on the production of glycoxylic acid by aspergillus niger]. 19664956477
lysosomes in type ii glycogenosis. changes during administration of extract from aspergillus niger. 19675234586
[on the toxicity exercised by zn++ and cd++ in the growth of aspergillus niger, the antagonism of these ions and mg++-zn++-cd++ interaction]. 19674965033
[kinetic studies of transaminations of keto-analogues of valine and isoleucine in aspergillus niger]. 19674969634
[studies on glucose oxidase of aspergillus niger. 3. mechanism of inhibitory action of nh4+ ion on the induction of this enzyme]. 19674383671
[sedimentation and diffusion coefficients from the transport of enzymatic activities. application of the method of determination to the glucose oxidases from penicillium notatum (notatin) and aspergillus niger (nigerin)]. 19676030353
effect of glucamylase on tissue glycogen and tissue glucamylase in the rat.1. a fungal glucamylase (alpha-1,4-glucan glucohydrolase, ec 3.2.1.3) from aspergillus niger depresses liver glycogen stores after intraperitoneal injection into the rat. the injected enzyme rapidly disappears (within about 8hr.) from the serum; less than 1% is excreted in the urine, but it is rapidly taken up in the liver, spleen, kidney, cardiac and skeletal muscle. elevated glucamylase concentrations could be demonstrated in liver and spleen tissues for 1-4 days after injection, but in kidney ...19676032976
resistivity of spores to ultraviolet and gamma radiation while exposed to ultrahigh vacuum or at atmospheric pressure.viability studies were conducted on microbial spores subjected to ultrahigh vacuum (uhv) in the 10(-9) to 10(-10) torr range. after 5 to 7 days in vacuum, they were exposed to ultraviolet (uv) or to gamma radiation either while still under vacuum or in the presence of dried air. among the four test organisms subjected to uhv and ultraviolet radiation, aspergillus niger was the most resistant; bacillus megaterium, b. subtilis var. niger, and b. stearothermophilus were about equally less resistant ...19676035043
antifungal activity of isothiocyanates and related compounds. i. naturally occurring isothiocyanates and their analogues.data are presented concerning the antifungal activity of 11 natural isothiocyanates and 27 synthetized analogues in aspergillus niger, penicillium cyclopium, and rhizopus oryzae, as well as in 13 additional saprophytic and parasitic fungi. a remarkable antifungal activity was observed in some analogues of benzylisothiocyanate and beta-phenylethylisothiocyanate. the latter-mentioned compounds have not been described previously. in the group of benzylisothiocyanates, a correlation, which was inver ...19676049294
antifungal activity of isothiocyanates and related compounds. ii. mononuclear aromatic isothiocyanates.antifungal activity on aspergillus niger, penicillium cyclopium, and rhizopus oryzae, as well as on additional saprophytic and parasitic fungi, was determined in 57 substituted derivatives of phenylisothiocyanate. most of the investigated compounds displayed rather equal activity against the three mentioned fungi, in contradistinction to the analogues of natural benzyl- and beta-phenylethylisothiocyanate with their characteristic low activity against r. oryzae. differences occurred in the type o ...19676049295
evidence for a buried location of nigeran in the cell wall of aspergillus niger. 19676052487
the parasitism of the mould penicillium purpurogenum on aspergillus niger. i. isolation of cell walls from the mycelium of aspergillus niger grown by the surface method. 19676053292
the parasitism of the mould penicillium purpurogenum on aspergillus niger. ii. the course of the lysis and some properties of the crude preparation of the enzymolytic system. 19676053293
the parasitism of the mould penicillium purpurogenum on aspergillus niger. 3. the determination of the part of some enzymes of penicillium purpurogenum in the mycelium lysis of the mould aspergillus niger. 19676056766
the parasitism of the mould penicillium purpurogenum on aspergillus niger. iv. the limitation of parasitic action. 19676056767
degradation of salicylate by aspergillus niger. 19676057252
the conversion of tryptophan to 2,3-dihydroxybenzoic acid and catechol by aspergillus niger. 19676064579
a possible role of hydroxypyruvic acid reductase in growth of a. niger. 19676066161
o-pyrocatechiuc acid carboxy-lyase from aspergillus niger. 19676066253
the isolation and characterization of asperenone, a new phenylpolyene from aspergillus niger. 19676073032
steroids and other factors influenceing the accumulation of asperenone and fermentation acids by aspergillus niger in replacement cultures. 19676073033
genetic analysis by means of the parasexual cycle in aspergillus niger. 19676075182
enzymic glucosylation of phenols.1. a transglucosylase fraction has been obtained from the mycelium of aspergillus niger. 2. the preparation will transfer alpha-d-glucopyranosyl residues from maltose and other alpha-d-glucopyranosides to phenolic and alcoholic hydroxyl groups and to carboxylic acid groups. 3. alpha-isomaltosides and alpha-maltosides are formed when resorcinol and catechol are used as acceptors. 4. ph precipitation and deae-cellulose chromatography were used to resolve the activity into two fractions. the proper ...19675584008
[endopolyglycosidase from aspergillus niger hydrolysing glucomannans from eremuruses]. 19675599805
[attempt to label biotin with sulfur-35 by aspergillus niger]. 19675624130
[acid metabolism in aspergillus niger. vi. interpretation of the relationship between c-n ratio in the end substrate and the accumulation of oxalic acid]. 19675633286
oxidative phosphorylation and respiratory control in mitochondria from aspergillus niger.1. tightly coupled mitochondria were isolated from aspergillus niger by using an all-glass homogenizer followed by differential centrifugation. 2. the mitochondria oxidized the common intermediates of the tricarboxylic acid cycle, nadh(2) and the ascorbate-tetramethyl-p-phenylenediamine system. 3. high p/o ratios and control of respiration by adp were obtained with all substrates tested. the average p/o ratios observed were: 1.5-1.8 with succinate as substrate [respiratory control ratio (rc) 2-4 ...19674292772
rotenone and amytal insensitive coupled oxidation of nadh by mitochondria from aspergillus niger. 19674294221
[research on glucose oxidase in aspergillus niger. ii. demonstration of the induction of that enzyme; conditions of its biosynthesis]. 19674234315
induction of sorbitol dehydrogenase by sorbitol in aspergillus niger. 19674299160
[studies on the role of silicic acid in the development of higher plants].germanium acid, a specific inhibitor of the silicic acid metabolism in diatoms, inhibits the growth of sinapis alba, lemna minor, wolffia arrhiza, nicotiana tabacum, tradescantia spec, zinnia elegans, and secale cereale when applied in the same concentrations as those used in the case of diatoms (15-75 μg geo2/ml medium). the growth of aspergillus niger, phycomyces blakesleanus, escherichia coli k 12, euglena gracilis and pandorina morum is not influenced by these and higher concentrations of ge ...196724549376
action mechanism of glucose oxidase of aspergillus niger. 19684299571
survey of microorganism for the production of extracellular phytase.a culture enrichment technique was used to isolate phytase-producing microorganisms. also, microorganisms from various culture collections were tested for their phytase-producing ability. a number of the aspergillus niger group produced extracellular phytase which dephosphorylated calcium phytate in acidic solution. a soil isolate, a. ficuum nrrl 3135, produced the most active phytase in a cornstarch-based medium. production of phytase was strongly repressed by inorganic phosphates and required ...19684300171
chemical and oxidative properties of coupled mitochondria from aspergillus niger. 19684300397
respiratory components of aspergillus niger mitochondria.the respiratory components of tightly coupled mitochondria from the filamentous fungus aspergillus niger were studied. cytochromes a + a(3), b, and c + c(1) were identified by difference spectra. the cytochrome spectra were qualitatively similar to yeast and rat liver mitochondria. the mitochondria contained, per gram of protein, an average of 2.9 and 7.0 mumoles of ubiquinone and nicotinamide adenine dinucleotide, respectively.19684302298
comparative mitochondrial function in two strains of aspergillus niger. 19684303003
[research on the glucose oxidase of aspergillus niger. iv. role of various amino acids in the induction of this enzyme]. 19684236647
genetics of citric acid producinig strains of aspergillus niger. i. citric acid synthesis by morphological mutants induced iwth uv. 19684178171
comparative studies on the glucose oxidases of aspergillus niger and penicillium amagasakiense. 19684297627
aspergillus niger technique for the bioassay of manganese. 19685640230
anthranilic acid hydroxylase from aspergillus niger. 19685656066
some observations on the aspergillin of aspergillus niger. 19685661613
some factors affecting the formation of protoplasts in aspergillus niger. 19685672585
induction of asexual sporulation in aspergillus niger in submerged liquid culture. 19685677992
electron microscopy of swelling and germinating conidiospores of aspergillus niger. 19685679663
metabolism of dethiobiotin in aspergillus niger. 19685679971
systemic aspergillosis caused by aspergillus niger after open-heart surgery. 19685694155
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