Publications

detection of the messenger rna encoding for the ferredoxin-dependent glutamate synthase in maize leaf.	ferredoxin-dependent glutamate synthase (ec 1.4.7.1), glutamate oxoglutarate aminotransferase (glutamate synthase) (gogat) messenger rna was extracted from maize (zea mays l.) leaves and partially purified through oligo(dt)-cellulose chromatography and ultracentrifugation in a sucrose gradient. mrna were translated in vitro using a reticulocyte system. the glutamate synthase subunit was characterized by immunoprecipitation with antibodies raised against the rice (oryza sativa l.) ferredoxin-glut ...	1986	16664732
non-proline-accumulating rice mutants resistant to hydroxy-l-proline.	three rice (oryza sativa l.) mutants resistant to hydroxy-l-proline (hyp), hyp 101, hyp 202 and hyp 203, were selected from an ethylene imine mutagenized m2 population of the original variety, 'nipponbare', and their biochemical and genetical characteristics were investigated. the sensitivity of the mutants to hyp could be clearly differentiated from that of the original variety when seeds were germinated and cultured with 10(-4)∼10(-3) m hyp for 10 days. a difference in hyp sensitivity was also ...	1986	24247838
in vitro characterization of salt stress effects and the selection of salt tolerant plants in rice (oryza sativa l.).	the response of plant cells to salt stress was studied on embryo derived calli of rice (oryza sativa l.) in order to identify cellular phenotypes associated with the stress. the feasability of selecting salt tolerant callus and its subsequent regeneration to plants was also studied. callus was grown on agar-solidified media containing 0%, 1% and 2% (w/v) nacl for 24 days. parameters such as fresh weight, dry weight, soluble protein and proline content were measured. the callus growth decreased m ...	1986	24247613
seed dormancy in red rice : v. response to azide, hydroxylamine, and cyanide.	the activity of nan(3) (0.5 millimolar), hydroxylamine-hcl (10-18 millimolar), and potassium cyanide (1 millimolar) as dormancy-breaking agents of dehulled red rice (oryza sativa) is ph-dependent such that medium ph values favoring formation of the uncharged chemical species resulted in the highest germination percentages. there was no promotive effect of ph itself in the range of 3 to 10. the minimum contact times for maximum response (>/=90% germination) to nan(3), kcn, and nh(2)oh-hcl are 8 h ...	1986	16664656
a comparison of the submergence response of deepwater and non-deepwater rice.	twelve cultivars of rice (oryza sativa l.), representing deepwater, short-statured, and semidwarf types, were tested for their response to submergence. the magnitude of the response varied between cultivars; however, all cultivars responded to submergence by rapid growth once internodal elongation had started. three of these cultivars were tested for elongation capacity at four ages. the deepwater rice was capable of rapid internodal elongation in response to submergence at 4 weeks of age. growt ...	1986	16664647
chilling sensitivity in oryza sativa: the role of protein phosphorylation in protection against photoinhibition.	the effects of exposure to low temperature on photosynthesis and protein phosphorylation in chilling-sensitive and cold-tolerant plant species were compared. chilling temperatures resulted in light-dependent loss of photosynthetic electron transport in chilling-sensitive rice (oryza sativa l.) but not in cold-tolerant barley (hordeum vulgare l.). brief exposure to chilling temperatures (0-15 degrees c, 10 min) did not cause a significant difference in photosynthetic o(2) evolution capacity in vi ...	1986	16664636
inactivation of alcohol dehydrogenase in rice seedlings is related to a microsomal fatty acid alpha-oxidation system.	the selective inactivation of alcohol dehydrogenase by the inactivator found in the microsomal fraction of rice (oryza sativa) seedlings growing in air (shimomura, s. & beevers, h. (1983) plant physiol. 71, 736-741; 742-746) was further studied. this inactivation was found to be essentially dependent on the presence of free fatty acids. the specificity for fatty acids and the inhibitory effects of imidazole, 2-hydroxyfatty acids and dithiothreitol on the inactivation were all consistent with the ...	1986	2939066
nucleotide sequence of the rice cytochrome f gene and the presence of sequence variation near this gene.	the rice (oryza sativa l. var. labelle) chloroplast (cp) gene encoding cytochrome f has been isolated and sequenced. the coding region of this rice gene displays 95.1%, 85.3% and 85.2% nucleotide sequence homology with that of wheat, pea and spinach, respectively. to examine the cpdna sequence variation in rice, cpdna from labelle and its parents, belle patna and dawn was compared. using the cytochrome f gene as the probe for hybridization, we found several differences in the size and number of ...	1986	2884170
carbon isotope ratios demonstrate carbon flux from c(4) host to c(3) parasite.	carbon isotope ratios of mature leaves from the c(3) angiosperm root hemiparasites striga hermonthica (del.) benth (-26.7 per thousand) and s. asiatica (l.) kuntze (-25.6 per thousand) were more negative than their c(4) host, sorghum (sorghum bicolor [l.] moench cv csh1), (-13.5 per thousand). however, in young photosynthetically incompetent plants of s. hermonthica this difference was reduced to less than 1 per thousand. differences between the carbon isotope ratios of two c(3)-c(3) association ...	1987	16665818
genetic and biochemical basis of scent in rice (oryza sativa l.).	the inheritance and biochemical basis of scent in rice was studied in the f2 population along with the f1 and its two parents, scented and non-scented 'pokura' rice strains. the f1 plants were found to be nonscented while the f2 plants seggregated into a 3∶1 ratio (non-scented: scented). in scented f2 seggregants and in the scented parental strain, a fast moving esterase isozyme, rf 0.9, is missing whereas it is present in all nonscented f2 seggregants, f1s, and in the non-scented parent. this s ...	1987	24241193
field performance and cytology of protoplast-derived rice (oryza sativa): high yield and low degree of variation of four japonica cultivars.	protoplast-derived rice plants of four japanese cultivars, nipponbare, fujisaka 5, norin 14 and iwaimochi were individually cultivated in a submerged paddy field. they exhibited more stems, which resulted in more panicles than respective control plants. other characteristics of protoplast-derived plants were (compared with controls): a slightly shorter or similar culm length, fewer spikelets per panicle, slightly lower seed fertility and similar or lighter 1,000 kernel weight. grain yield of pro ...	1987	24240226
amylase activity and growth in internodes of deepwater rice.	isoelectrofocusing, product analysis, thermal denaturation studies and affinity chromatography on cycloheptaamylose-sephadex were used to identify the amylolytic enzymes in internodes of deepwater rice (oryza sativa l.). amylolytic activity in internodes of deepwater rice consists of α-amylase (sometimes separated into two isoforms) and of β-amylase. during submergence of whole plants, α-amylase activity increases in young, growing internodes, but β-amylase activity declines. although non-growin ...	1987	24225795
position of anthers at plating and its influence on anther callusing in rice.	effect of position of anthers at plating in relation to their ability to form callus was studied in rice (oryza sativa var. taipei 309). among the callusing anthers, about 60% were those positioned on edge with one lobe in contact with the medium while the rest were flat with both lobes touching the medium. anthers in both positions produced calli and regenerated green plants.	1987	24248769
seed dormancy in red rice : vi. monocarboxylic acids: a new class of ph-dependent germination stimulants.	the weak acid character of many previously identified, but otherwise chemically dissimilar, dormancy-breaking compounds may contribute to their physiological activity. to test this idea, short chain monocarboxylic acids of one to six carbons, for which no previous reports of such activity exist, were incubated with dormant, dehulled red rice (oryza sativa) seeds. greater than 90% germination was observed after 24 hours of imbibition with 19 millimolar formic, 53 millimolar acetic, 20 millimolar ...	1987	16665509
in vitro segregation of marker genes in anther cultures of rice.	segregation of genes controlling expression of anthocyanin pigmentation in rice (oryza sativa l. subsp. indica) leaf blade and leaf sheath was examined in the microspore-derived plants. the segregation pattern of marker genes was found to fit closely the expected gametic segregation ratios among microspore-derived green as well as albino plants. microspore-derived in vitro regenerated plants expressed genetic traits similar to seedlings. the results indicate that the germ cell culture technique ...	1987	3610948
differentiation of rice varieties by electrophoresis of embryo protein.	variations in the embryo proteins separated by sds-page have been observed in 43 cultivated varieties of oryza sativa l. cluster and discriminant analysis applied to both protein components and morphological characters indicate that knowledge of the differences in embryo proteins can improve our understanding of genetic affinity and make it easier to differentiate between varieties of similar genetic backgrounds.	1987	24241569
fatty acids of rice coleoptiles in air and anoxia.	the metabolism of lipids, like that of other components, was adversely and strongly affected when rice (oryza sativa l.) coleoptiles were grown anaerobically. in aerobic coleoptiles, the amounts of total fatty acid, phospholipid, and total lipid per coleoptile increased by 2.5- to 3-fold between days three and seven, whereas under anoxia, the increases were all less than 60%. the total amount of lipid at day seven in anoxia was less than 30% of that in air. in air, the total fatty acid content a ...	1987	16665478
an evaluation of 2,5-norbornadiene as a reversible inhibitor of ethylene action in deepwater rice.	partial submergence of deepwater rice (oryza sativa l. cv habiganj aman ii) elicits three responses: enhancement of internodal elongation, inhibition of leaf growth, and promotion of adventitious root formation. all three responses can be induced in isolated stem sections by treatment with ethylene. dose-response curves indicate that the responses are linearly related to the logarithm of the ethylene concentration over two orders of magnitude. application of the cyclic olefin 2,5-norbornadiene ( ...	1987	16665450
in vivo 1-aminocyclopropane-1-carboxylate synthase activity in internodes of deepwater rice : enhancement by submergence and low oxygen levels.	inasmuch as the activity of 1-aminocyclopropane-1-carboxylate (acc) synthase cannot be measured in homogenates of deepwater rice internodes (oryza sativa l.), we have employed an in vivo assay to determine the activity of this enzyme. this assay is based on the accumulation of acc in tissue kept under n(2). submergence of whole plants or stem sections containing the uppermost, developing internode enhances the in vivo activity of acc synthase in the stem. this stimulation of in vivo acc-synthase ...	1987	16665431
effect of sugar mill effluent on oxygen uptake and carbon dioxide output of rice (oryza sativa l.c.v. mushoori) seedlings.	the effects of a sugar mill effluent on respiration of rice (oryza sativa l.c.v. mushoori) seedlings have been investigated. each experiment consisted of two parts, the effect of various concentrations of the effluent and the time-dependent changes induced by the undiluted effluent. a marked increase in the rate of respiration was noticed upon treatment with various concentrations of the effluent. the time-dependent changes in the respiratory rate were about threefold with the 12 hr of treatment ...	1987	3107978
nuclear dna amplification in cultured cells of oryza sativa l.	highly repeated nuclear dna sequences from suspension cultured cells of oryza sativa l. cv. 'roncarolo' have been cloned in pbr322. ten clones with specific digestion patterns have been randomly selected. nine sequences appear to be organized in a clustered tandem array while one is interpersed in the rice genome. the clones have been used to gather information on: (a) their modulation in cultured cells as compared to whole plant and (b) their distribution in different rice cultivars belonging t ...	1987	24241458
a 5-methyltryptophan resistant rice mutant, mtr1, selected in tissue culture.	cell lines resistant to tryptophan analogue 5-methyltryptophan (5mt) were selected in seed-derived calli of oryza sativa l. var. 'norin 8'. plants were regenerated (r1 from one selected callus line (mtr1). in three out of the six r1 plants, 5mt resistance was inherited in the r2 and r3 generations as a dominant nuclear mutation. segregation ratios in the progeny of heterozygous plants were 1∶1. morphological and fertility variation seen in some of the r2 plants were not correlated with 5-methylt ...	1987	24241455
isozymes and classification of asian rice varieties.	enzyme variation detected by starch gel electrophoresis was used to investigate the genetic structure of oryza sativa l. species. fifteen polymorphic loci coding for 8 enzymes were surveyed among 1688 traditional rices from asia. multivariate analysis of the data resulted in identification of six varietal groups, with two major ones, groups i and vi, two minor ones, groups ii and v, and two satellite ones, groups iii and iv. group i is found throughout tropical asia; it encompasses most aman ric ...	1987	24241451
plant regeneration from in vitro cultures of anthers and mature seeds of rice (oryza sativa l.) cv. basmati-370.	pollen plants were obtained from anther-derived calli of the indica rice variety basmati-370. anther-response (anthers producing pollen derived calli) and plant regeneration frequency from the pollen derived calli. was very low. donor plants which flowered at the average max/min. temperature of 34.2°/23.3°c gave a significantly higher anther-response to in vitro techniques, than did those which flowered at 29.1°/16.4°c. somatic callus induction and subsequent plant regeneration was readily obtai ...	1987	24248447
genetic analysis of waxy locus in rice (oryza sativa l.).	inheritance of waxy locus was studied in crosses of a waxy variety with four non-waxy parents having high-, intermediate-, low- or very low-amylose content. the analysis for amylose content was done on a single grain basis in parents, f1, f2, b1f1, and b2f1 seeds. the waxy parent lacking synthesis of amylose content was found to differ from the ones having high-, intermediate-, low- or very low-amylose content by one gene with major effect. dosage effects for amylose content were observed to hav ...	1987	24241102
phosphorylation of thylakoid proteins of oryza sativa: in vitro characterization and effects of chilling temperatures.	the phosphorylation of thylakoid proteins of rice (oryza sativa l.) was studied in vitro using [gamma-(32)p]atp. several thylakoid proteins are labeled, including the light-harvesting complex of photosystem ii. protein phosphorylation is sensitive to temperature, ph, and adp, atp, and divalent cation concentrations. in the range ph 7 to 8.2, phosphorylation of the light-harvesting polypeptides declines above ph 7.5, whereas labeling of several other thylakoid polypeptides increases. increasing d ...	1987	16665262
differences between indica and japonica rice varieties in co2 exchange rates in response to leaf nitrogen and temperature.	four indica and five japonica varieties of rice (oryza sativa l.) were examined to elucidate their differences in photosynthetic activity and dark respiratory rate as influenced by leaf nitrogen levels and temperatures. the photosynthetic rates of single leaf showed correlations with total nitrogen and soluble protein contents in the leaves. respiratory rate was also positively correlated with the leaf nitrogen content. when compared at the same level of leaf nitrogen or soluble protein content, ...	1987	24430670
hirschmanniella spp. in rice fields of vietnam.	root and soil samples from one-crop and two-crop rice fields were collected in a survey for hirschmanniella spp. in vietnam during 1978-80. hirschmanniella spp. were found in 50-78% of the soil samples and 98-100% of the root samples collected. population densities of nematodes in root systems were lowest at posttransplanting and highest at heading time. numbers of nematodes inside roots increased 20-22 times from transplanting to heading in fields with both crop sequences. population densities ...	1987	19290110
biochemical and immunological characterization of rice albumin.	rice albumin from oryza sativa (var. basmati 370) accounts for about 5% of the total seed proteins. a major fraction of rice albumin has been found to be a glycoprotein which is a monomer of 60 kd having iso-electric point 6.54. when rice albumin is digested with trypsin, it shows the presence of 24 peptides as against 28 peptides which were estimated from its amino acid composition. this indicates the presence of a few peptides which resemble each other in their charge and rf values. antibodies ...	1987	3620599
the effects of fruit juices and fruits on the absorption of iron from a rice meal.	the effects of the chemical composition of fruit juices and fruit on the absorption of iron from a rice (oryza sativa) meal were measured in 234 parous indian women, using the erythrocyte utilization of radioactive fe method. the corrected geometric mean fe absorptions with different juices varied between 0.040 and 0.129, with the variation correlating closely with the ascorbic acid contents of the juices (rs 0.838, p less than 0.01). ascorbic acid was not the only organic acid responsible for t ...	1987	3593665
isolation, molecular and biological properties of a lectin from rice embryo: relationship with wheat germ agglutinin properties.	rice lectin (oryza sativa, var. balilla 28) was purified from defatted embryos by aqueous acid extraction at ph 1.3 followed by ammonium sulfate precipitation between 2 and 4 m, affinity chromatography on agarose-p-aminophenyl-beta-d-n-acetylglucosamine, and gel filtration on aca 54. the homogeneity of the lectin was checked by polyacrylamide gel electrophoresis, gel filtration, and immunodiffusion. the amino acid analysis revealed a high half-cystine content (9%) and a low aromatic and hydropho ...	1987	3688888
investigations of the host range of the corn cyst nematode, heterodera zeae, from maryland.	the host range of the corn cyst nematode, heterodera zeae, recently detected in maryland, was investigated. a total of 269 plant entries, representing 68 families, 172 genera, and 204 species, was inoculated with cysts or a mixture of eggs and second-stage juveniles of h. zeae. the host range of the maryland population of h. zeae was limited to plants of the gramineae and included 11 tribes, 33 genera, 42 species, and 77 entries. all 22 corn (zea mays) cultivars tested were hosts. other economic ...	1987	19290286
evaluation of pseudomonas fluorescens for suppression of sheath rot disease and for enhancement of grain yields in rice (oryza sativa l.).	pseudomonas fluorescens strains antagonistic to sarocladium oryzae, the sheath rot (sh-r) pathogen of rice (oryza sativa l.), were evaluated in greenhouse and field tests for suppression of sh-r severity and enhancement of grain yields of rice. imprints of rice seedlings and a direct-observation technique of staining roots with fluorochromes confirmed the association of p. fluorescens with roots and the ability of the strain to move along shoot tips. in greenhouse tests, p. fluorescens-treated r ...	1987	16347428
synthesis of the low molecular weight heat shock proteins in plants.	heat shock of living tissue induces the synthesis of a unique group of proteins, the heat shock proteins. in plants, the major group of heat shock proteins has a molecular mass of 15 to 25 kilodaltons. accumulation of these proteins to stainable levels has been reported in only a few species. to examine accumulation of the low molecular weight heat shock proteins in a broader range of species, two-dimensional electrophoresis was used to resolve total protein from the following species: soybean ( ...	1987	16665553
physiological studies of a synthetic gibberellin-like bioregulator: ii. effect of site of application on biological activity.	the biological activity of the synthetic gibberellin agonist ac-94,377 (1-[3-chlorophthalimido]-cyclohexanecarboxamide) in certain plants is strictly dependent on the site of application. root application of ac-94,377 at concentrations greater than or equal to 1 micromolar to seedlings of dwarf corn (zea mays l. var d(5)), dwarf rice (oryza sativa l. cv tan-ginbozu), and sunflower (helianthus annuus l. cv nk265) seedlings resulted in readily measurable gibberellin-like biological activity. appli ...	1987	16665563
isozymic variability of traditional rice (oryza sativa l.) in africa.	eight hundred and thirtyone traditional varieties of rice, oryza sativa l., were collected in africa and analysed for their isozymic variability on 15 enzymatic systems, representing 37 presumed loci. there appears to be a correlation between the type of rice growing and the two groups indica and japonica. the degree of genetic diversity is nearly equal in african rice and the asian one. alleles due to introgression or mutational events were identified. the results suggest that the evolution of ...	1987	24241190
transient expression of foreign genes in rice, wheat and soybean cells following particle bombardment.	the development of an efficient transformation system is a prerequisite for the molecular analysis of gene expression in plants. in crop plants, this development has been hindered by difficulties encountered both in whole plant regeneration from protoplasts and in the general insusceptibility of monocots to agrobacterium-mediated transformation. we have circumvented these difficulties by transferring foreign genes directly into the intact cells (with cell walls) of three important crop plants in ...	1988	24272400
trichodorus petrusalberti n. sp. (nematoda: trichodoridae) from rice with additional notes on the morphology of t. sanniae and t. rinae.	a new species in the family trichodoridae, from the rhizosphere of rice (oryza sativa l.) in northern natal, south africa, is described and illustrated. trichodorus petrusalberti n. sp. resembles t. taylori de waele, mancini, roca, and lamberti, 1982, t. hooperi loof, 1973, and t. complexus rahman, jairajpuri, and ahmad, 1985, from which it is distinguished by the shape of the spicules. it is distinguished from the former two species by the nonthickened terminal cuticle in the males and by the s ...	1988	19290188
rapid elicitation of second messengers by nanomolar doses of triacontanol and octacosanol.	triacontanol (tria) increases the dry weight and alters the metabolism of rice (oryza sativa l.) seedlings within 10 min of application to either the shoots or roots. this activity is prevented if octacosanol (octa, c28 primary alcohol) is applied with the tria on the roots or shoots. triacontanol activity is also stopped if the octa is applied at least 1 min before the tria on the opposite part of the seedling.triacontanol rapidly elicits a second messenger that moves rapidly throughout the pla ...	1988	24226183
chromosomal locations of ten isozyme loci in rice (oryza sativa l.) through trisomic analysis.	chromosomal locations of 10 isozyme loci in rice (oryza sativa l.) were determined through trisomic analysis. all 10 genes produced altered allozyme banding patterns in specific f1 trisomics. this served as the primary source of evidence for chromosome locations of est-5, icd-1, acp-1, and pgd-1. the locations of amp-1, amp-2, amp-4, pox-5, got-1, and cat-1 were further confirmed from segregation data in bc1 generations, as the ratios deviated significantly from 1:1 in the critical trisomics but ...	1988	3408480
temperature and the cell cycle.	during the period between successive divisions, a cell traverses three stages of interphase: g1 (pre-synthetic interphase), s-phase (dna synthetic interphase) and g2 (post-synthetic interphase). the time taken for all cells in a meristem to divide (the cell doubling time (cdt] decreases in response to an increase in temperature. for example, the cdt in root meristems of zea mays decreases 21-fold as the temperature is increased from 3 to 25 degrees c. whether all phases of the cell cycle alter p ...	1988	3077857
molecular mapping of rice chromosomes.	we report the construction of an rflp genetic map of rice (oryza sativa) chromosomes. the map is comprised of 135 loci corresponding to clones selected from a psti genomic library. this molecular map covers 1,389 cm of the rice genome and exceeds the current classical maps by more than 20%. the map was generated from f2 segregation data (50 individuals) from a cross between an indica and javanica rice cultivar. primary trisomics were used to assign linkage groups to each of the 12 rice chromosom ...	1988	24232389
distribution of mitochondrial plasmid-like dna in cultivated rice (oryza sativa l.) and its relationship with varietal groups.	mitochondrial (mt) plasmid-like dna was found in most of more than 100 rice cultivars (oryza sativa l.) by the use of 0.7% agarose gel electrophoresis (age). the dna varied in molecular weight and number. by electron microscopy, small circular dnas of different sizes could be detected in addition to the dnas of high molecular weight, even in cultivars in which mt plasmid-like dna was not detected by age. the detection of the mt plasmid-like dnas by age did not depend on their presence or absence ...	1988	24232388
sequence of the chloroplast psbf gene encoding the photosystem ii 10 kda phosphoprotein from oryza sativa l.		1988	3057446
structure, expression, and heterogeneity of the rice seed prolamines.	by screening two rice (oryza sativa l.) seed cdna libraries, recombinant cdna clones encoding the rice prolamine seed storage protein were isolated. based on cross-hybridization and restriction enzyme map analyses, these clones can be divided into two homology classes. all clones contain a single open reading frame encoding a putative rice prolamine precursor (molecular weight = 17,200) possessing a typical 14-amino acid signal peptide. the deduced primary structures of both types of prolamine p ...	1988	16666363
production of kanamycin resistant rice tissues following dna uptake into protoplasts.	rice protoplasts (oryza sativa l. v taipei 309) have been transformed to kanamycin resistance following uptake of pcamvneo induced by electroporation, peg and peg combined with electroporation. protoplast-derived colonies selected on medium containing 100 μg/ml of kanamycin expressed nptii activity, and contained dna that hybridised to a 1.0 kb bamhi fragment of pcamvneo carrying the nptii gene. expression of the transformation frequency in relative terms (number of kanamycin resistant colonies ...	1988	24240260
molecular analysis of organelle dna of different subspecies of rice and the genomic stability of mtdna in tissue cultured cells of rice.	chloroplast (ct) and mitochondrial (mt) dnas were isolated from two subspecies of rice (oryza sativa), japonica (calrose 76) and indica (pi353705) and compared by restriction endonuclease fragment pattern analysis. similarly, pi353705 (a5) mtdna was also compared with the mtdna of its long term tissue cultured line, bl2. variation in the ctdna of the 2 subspecies was detected with two (avai and bgli) of the 11 restriction endonucleases tested, whereas their mtdnas showed considerable variation w ...	1988	24232271
the biophysical basis of elongation growth in internodes of deepwater rice.	partial submergence induces rapid internodal elongation in deepwater rice (oryza sativa l., cv habiganj aman ii). we measured in vivo extensibility, tissue tension, hydraulic conductance and osmotic potential in the region of cell elongation in the uppermost internode. the in vivo extensibility of the internode, measured by stretching of living tissue with a custom-made constant stress extensiometer, rose rapidly following submergence of the plant. both the elastic (e(el)) and plastic (e(pl)) ex ...	1988	16666308
factors influencing protoplast viability of suspension-cultured rice cells during isolation process.	callus cells of rice (oryza sativa l.) that were actively dividing in suspension culture had lost the ability to divide during the isolation process of protoplasts. factors influencing the protoplast viability were examined using highly purified preparations of cellulase c(1), xylanase, and pectin lyase, which were essential enzymes for the isolation of protoplasts from the rice cells. the treatment of the cells with xylanase and pectin lyase, both of which are macerating enzymes, caused cellula ...	1988	16666273
interaction of a gibberellin-induced factor with the upstream region of an alpha-amylase gene in rice aleurone tissue.	the interaction between the dna sequences of an alpha-amylase (ec 3.2.1.1) gene and a tissue-specific factor induced in rice (oryza sativa l.) aleurone tissue by gibberellin was studied. dna mobility-shift during electrophoresis indicated that a 500-base-pair sequence (hs500) of a rice alpha-amylase genomic clone (osamy-a) specifically interacted with a factor from gibberellin-induced rice aleurone tissue. the amount of complex formed between the hs500 dna fragment and the gibberellin-induced fa ...	1988	3261867
chromosomal location of genes conditioning low amylose content of endosperm starches in rice, oryza sativa l.	eight dull mutants that lower the amylose content of rice endosperm as well as waxy mutant and a cultivar with common grains were crossed in a diallele manner. the amylose content of f1 and f2 seeds was determined on the basis of single grain analysis. it was concluded that the low amylose content of dull mutants is under monogenic recessive control. alleles for low amylose content are located at five loci designated as du-1, du-2, du-3, du-4 and du-5. these loci are independent of wx locus loca ...	1988	24232104
differences between wheat and rice in the enzymic properties of ribulose-1,5-bisphosphate carboxylase/oxygenase and the relationship to photosynthetic gas exchange.	the kinetic parameters of ribulose-1,5-bisphosphate (rubp) carboxylase/oxygenase (ec 4.1.1.39) in wheat (triticum aestivum l.) and rice (oryza sativa l.) were determined by rapidly assaying the leaf extracts. the respective k m and v max values for carboxylase and oxygenase activities were significantly higher for wheat than for rice. in particular, the differences in the v max values between the two species were greater. when the net activity of co2 exchange was calculated at the physiological ...	1988	24221414
interaction of asparagine-linked oligosaccharides with an immobilized rice (oryza sativa) lectin column.	the carbohydrate-binding specificity of rice (oryza sativa) lectin was investigated by testing the ability of radioactively labelled glycopeptides and oligosaccharides to bind to a rice lectin-sepharose 4b column. rice lectin binds asparagine-linked oligosaccharides through the core nn'-diacetylchitobiose moiety. whereas beta 1-4-mannose enhances the binding strength only to a small extent, alpha 1-3-linked core mannose increases it considerably. a core alpha 1-6-linked mannose residue has a sli ...	1988	3355507
isolation and hypoglycemic activity of oryzabrans a, b, c and d, glycans of oryza sativa bran.		1988	3375329
effect of gamma-irradiated sludge on the growth and yield of rice (oryza sativa l. var. gr-3).	the effects of gamma-irradiated sludge on the growth and yield of rice (oryza sativa l. var. gr-3) in pot cultures have been studied. compared to plants grown only in soil, shoot length, root length, fresh weight, dry weight, total proteins, total soluble sugars, starch and chlorophyll content of plants grown in soil supplemented with unirradiated or gamma-irradiated sludge were found to be significantly increased. irradiation of sludge significantly stimulated the linear growth of shoot and roo ...	1988	15092639
inheritance of two endosperm protein loci in rice (oryza sativa l.).	previous studies indicated two types of phenotypic protein markers as two minor bands of sds-page for rice storage protein. a variant derived from a pakistani variety, dular, was found to show a mobility variant with band 11, a relatively faster-moving band as compared to band 10, while most of the other cultivated rices exhibited band 10 at a molecular weight of around 100-110 k. band 11 was also observed in several wild rice species. how this variant occurred is not known. another marker is ch ...	1989	24226007
selection of higher regenerative callus and change in isozyme pattern in rice (oryza sativa l.).	calli were initiated from seedling roots in rice (oryza sativa l. var. tadukan) and subcultured at 45-day intervals on ms medium supplemented with 2 mg/l 2,4-d. sectors of callus which differentiated shoot meristems (green spots) under the same 2,4-d concentration were selected from the calli subcultured 90 days after initiation. the selection was continued for about 2 years. responses to 2,4-d between original and selected lines differed considerably, although differentiation was not generally ...	1989	24225824
effects of cold-treatment on protein synthesis and mrna levels in rice leaves.	the effects of cold on protein and rna metabolism in leaves of rice (oryza sativa l.) seedlings were investigated. treatment with a diurnal cycle of 15/10 degrees c or 11/6 degrees c for up to 1 week resulted in progressive changes in the protein synthesis pattern after in vivo labeling of intact rice leaves with [(35)s]methionine. these changes were reversed when the seedlings were returned to normal growth temperatures. while de novo accumulation of several abundant proteins was suppressed, so ...	1989	16667159
increased proteolysis of senescing rice leaves in the presence of nacl and kcl.	nacl and kcl enhanced the degradation of chlorophylls and proteins in detached rice (oryza sativa) leaves in a concentration-dependent manner. ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) accounted for 73 to 80% of the protein lost by day 4 in the light. nacl at 50 millimolar increased proteolysis by 21% over the control in 4 days, but the addition of cycloheximide reduced the increase to about one-half. cycloheximide alone had no effect on proteolytic activity during this period. l ...	1989	16667137
polyamines in rice seedlings under oxygen-deficit stress.	incubation of 3-d-old seedlings of oryza sativa l. cv arborio under anaerobic conditions, leads to a large increase in the titer of free putrescine while aerobic incubation causes a slight decrease. after 2 days, the putrescine level is about 2.5 times greater without oxygen than in air. the rice coleoptile also accumulates a large amount of bound putrescine and, to a lesser extent, spermidine and spermine (mainly as acid-soluble conjugates). accumulation of conjugates in the roots is severely i ...	1989	16667132
three cdna sequences coding for glutamine synthetase polypeptides in oryza sativa l.		1989	2577497
development of salt tolerant lines of kdml and lpt rice cultivars through tissue culture.	salt tolerant lines of indica rice (oryza sativa l.) were selected out of kdml and lpt cultivars. the first selection was made in vitro by incorporating 1 or 2% nacl in the culture media. embryogenic calli from mature embryo were subjected to a salt stress for four weeks. regeneration rates after salt stress were reduced to 0.076% or less as against regeneration rates of 8.3 to 30% normally obtained for non-stressed conditions. seedlings of regenerants and of following generations were treated w ...	1989	24233365
polyamines as modulators of salt tolerance in rice cultivars.	the effect of nacl on the endogenous levels of diamine, putrescine and polyamines, spermidine and spermine, was studied in the shoot system of salt-tolerant and salt-sensitive lines of rice (oryza sativa l.) cultivars during three growth stages. salt stress increased the levels of diamine and polyamine in varying degrees among nine rice cultivars investigated. salt tolerant au1, co43, and csc1 were effective in maintaining high concentrations of spermidine and spermine, while the content of putr ...	1989	16667061
heavy metal tolerance of rice plants (oryza sativa l.) to some metal oxides at the critical levels.	the toxicity of metal oxides (cdo, zno, pbo) were compared with each other and the critical levels of metal uptake by rice plants were determined. the order of metal toxicity to rice plants is cdo greater than zno greater than pbo. the highest concentration obtained for unpolished rice was 4.97 micrograms/g at the cd 10,000 ppm in soil. this values is higher than every other we have seen in the reports for treatment with cdo. we are able to find out that the concentration of 10,000 ppm cd in the ...	1989	2600364
membrane-associated binding sites for indoleacetic acid in the rice coleoptile.	as described previously, the sensitivity of rice (oryza sativa l.) coleoptiles to auxin is modulated by oxygen. under anoxia, coleoptile elongation is insensitive to exogenously applied indole-3-acetic acid (iaa), whereas its sensitivity increases in air in the presence of the exogenous stimulus. here we report the presence of two independent classes of membrane-bound iaa-binding sites in air-grown coleoptiles. their binding activity is strictly correlated with the system's sensitivity to iaa. w ...	1989	24201521
structure and expression of the rice glutelin multigene family.	a near full-length cdna and three genomic clones for rice (oryza sativa l.) glutelin were isolated and studied. based on nucleic acid sequence and southern blot analyses, the three isolated glutelin genomic clones were representative members of three gene subfamilies each containing five to eight copies. a comparison of dna sequences displayed by relevant regions of these genomic clones showed that two subfamilies, represented by clones gt1 and gt2, were closely related and evolved by more recen ...	1989	2745459
developmental mutants showing abnormal organ differentiation in rice embryos.	zygotes of rice (oryza sativa l. cv taichung 65) were treated with 1.0 mm solution of the chemical mutagen n-methyl-n-nitrosourea. out of 1420 m2 lines, 28 single-locus recessive mutants on embryogenesis were identified. among them, we analyzed 11 mutants in the present study, which differentiated the shoot (plumule) and/or root (radicle) with abnormality. of the 11 mutants, two showed no shoot differentiation with normal root. on the other hand, we could not detect any mutant which exhibited a ...	1989	24227023
plant regeneration from indica rice (oryza sativa l.) protoplasts.	rice (oryza sativa l.) plants of the indica cultivar ir54 were regenerated from protoplasts. conditions were developed for isolating and purifying protoplasts from suspension cultures with protoplast yields ranging from 1·10(6) to 15·10(6) viable protoplasts/1 g fresh weight. protoplast viability after purification was generally over 90%. protoplasts were cultured in a slightly modified kao medium in a petri plate by placing them onto a millipore filter positioned on top of a feeder (nurse) cult ...	1989	24212899
the nucleotide sequences and expression of genes for the beta and epsilon subunits of atp synthase from rice (oryza sativa l.)	the genes for the beta and epsilon subunits of atp synthase were cloned from rice chloroplast dna. the nucleotide sequences and the deduced amino acid sequences of these genes were determined. the site of initiation of transcription was examined by s1-mapping analysis. the relation between stability of mrna and that of a stem and loop structure at the 3' end of mrna is discussed.	1989	2534354
plant regeneration from cytoplasmic hybrids of rice (oryza sativa l.).	we obtained cybrid plants by electrofusing γ-irradiated protoplasts of a cytoplasmic male-sterile line "a-58 cms" (oryza sativa l.) and iodoacetamide (ioa)-treated protoplasts of the fertile (normal) rice cultivar "fujiminori". the cybridity of the plants was confirmed by mitochondrial (mt) dna restriction endonuclease, and plasmid-like dna analyses, and by isozyme, cytological and morphological investigations. the chromosome number of the cybrid plants is 24.	1989	24232605
sugar content and activity of sucrose metabolism enzymes in milled rice grain.	most rice (oryza sativa l.) cultivars grown in the united states were selected for endosperm starch properties and not soluble sugar content. the minor pool of soluble sugar may affect the qualities of rice as a food. some cultivar variation in soluble sugar content was detected in milled grain, essentially the starchy endosperm, of long grain varieties. milled grain of cultivars lemont and texmati had a soluble sugar content of 0.21 and 0.35% (w/w), respectively, on a fresh weight basis. the do ...	1989	16666638
seed dormancy in red rice : vii. structure-activity studies of germination stimulants.	many chemically dissimilar substances break dormancy of seeds, but the relationship between chemical structure and physiological activity is unknown. in this study, the concentrations of organic acids, esters, aldehydes, alcohols, and inorganic weak acids required to elicit 50% germination of initially dormant, dehulled red rice seeds (oryza sativa) were determined. the activity of most substances was very highly and inversely correlated to lipophilicity as measured by octanol/water partition co ...	1989	16666635
establishment of a system of high-frequency embryogenesis from long-term cell suspension cultures of rice (oryza sativa l.).	suspension cultures which maintained embryogenic potency for more than 18 months were established from excised immature embryos of rice (oryza sativa l. cv. konansou). the cultures were subcultured every three days in n6 medium supplemented with proline (10 mm), casein hydrolysate (300 mg/l), sucrose (30 g/l) and 2,4-d (1 mg/l). the frequency of embryogenesis from the embryogenetic suspension cultures reached about 90% when cell clusters (about 1 mm in diameter) were transferred to a solid mediu ...	1989	24232530
long-term effects of land application of aqueous oil effluent on photosynthetic efficiency of certain varieties of oryza sativa l.		1989	2920225
mrnas and a cloned histone gene are differentially expressed during anther and pollen development in rice (oryza sativa l.).	spatial and temporal changes in the distribution of mrna sequences during anther and pollen development in rice (oryza sativa) were investigated by in situ hybridization with [3h]polyuridylic acid ([3h]poly(u)) and a cloned rice histone gene probe. annealing of sections with [3h]poly(u) showed that poly(a)-containing rna (poly(a)+rna) was uniformly distributed in the cells of the anther primordium. during the formation of the archesporial initial, the primary parietal cell, the primary sporogeno ...	1989	2476454
free proline contents in two different groups of rice mutants resistant to hydroxy-l-proline.	in four rice (oryza sativa l.) mutants resistant to hydroxy-l-proline (hyp), hyp101, hyp203, hyp205 and hyp210, and in their original variety, nipponbare, free proline and hyp contents in the seeds and in the 14-day-old seedlings have been determined. the four mutants can be divided into two groups: hyp101 and hyp203 are classified as to recessive gene and the levels of free proline are similar to that of the original variety; the second group includes mutants hyp205 and hyp210 where the hyp res ...	1989	24232472
the performance of three cultivars of rice grown near to, and distant from, a fertiliser plant.	the effects of three cultivars, two water regimes and two rates of applying nitrogen fertilisers were tested when studying the performance of rice (oryza sativa l.) near to, and distant from, a fertiliser plant emitting atmospherically dispersed pollutants. the atmosphere near the fertiliser plant had average daily peak concentrations of 144 and 210 microg m(-3) of so(2) and no(2), respectively. growth was less near to, than at a distance from, the fertiliser plant. on average it was decreased b ...	1989	15092427
[characterization of a cloned dna fragment of rice chloroplast and location rbcl gene in the recombinant plasmid].	the pure rice (oryza sativa, subsp. indica, cv. zhenshan 97b) chloroplast dna was digested by restriction enzyme bamhi and the resulting fragments were ligated to the bamhi site of pbr322. one recombinant plasmid which contains a 19.3kb insertel dna fragment was isolated from the clone bank and was named posb1. a physical map of the recombinant plasmid was constructed by cleavage with ten restriction endonucleases, and the gene rbcl was located on the posb1.	1989	2561754
[larval density of anopheles (n) albimanus wiedemann, 1821 in relation to phenology of oryza sativa (linnaeus) rice].	the larval density of anopheles (n) albimanus wiedemann, 1821, is linked with rice phenology in the two crops of the year (summer and winter). it was determined that a higher density agrees with the second stage of the vegetative cycle of the culture named beginning of shooting processes and the formation of the panicular primordium. the number of anopheline larvae was higher in the summer.	1989	2672175
the complete sequence of the rice (oryza sativa) chloroplast genome: intermolecular recombination between distinct trna genes accounts for a major plastid dna inversion during the evolution of the cereals.	the entire chloroplast genome of the monocot rice (oryza sativa) has been sequenced and comprises 134525 bp. predicted genes have been identified along with open reading frames (orfs) conserved between rice and the previously sequenced chloroplast genomes, a dicot, tobacco (nicotiana tabacum), and a liverwort (marchantia polymorpha). the same complement of 30 trna and 4 rrna genes has been conserved between rice and tobacco. most orfs extensively conserved between n. tabacum and m. polymorpha ar ...	1989	2770692
evaluation of selectable markers for rice transformation.	a variety of expression systems and selection régimes have been developed to transform plants such as tobacco, petunia, and tomato. we investigated several of these to determine whether the promoters and selectable markers used in dicotyledonous plants are suitable for selecting transformed rice callus. we compared transient expression driven by constitutive and regulated promoters in rice (oryza sativa) protoplasts and found that the 2' promoter of the octopine t-dna is approximately 3 to 4 tim ...	1989	16666739
artefactual origins of cyclic amp in higher plant tissues.	a highly sensitive radioimmunoassay has been used to determine the levels of adenosine 3',5'-cyclic monophosphate (camp) in five higher plants (lactuca sativa, helianthus annuus, oryza sativa, pinus pinaster, nicotiana tabacum). particular attention was paid to the three main sources of errors in the characterization of camp in plants: presence of interfering substances in plant tissues; possible artefactual formation of camp from endogenous atp during extraction, purification, and assay; and mi ...	1989	16667078
effects of ddt on the growth of crop plants.	the effects of ddt on the germination and growth of plants were studied using many crop species. of the species tested, oil-rich seeds of plants, such as peanut (arachis hypogaea) and mustard (brassica juncea), were more prone to ddt induced inhibition of germination and subsequent plant growth than cereals, pulses and fibre crops, like rice (oryza sativa), barley (hordeum vulgare), mung bean vigna radiata), pigeon pea (cajanus cajan) and cotton (gossypium hirsutum). studies with (14)c labelled ...	1989	15092369
photoinhibition at low temperature in chilling-sensitive and -resistant plants.	photoinhibition resulting from exposure at 7 degrees c to a moderate photon flux density (300 micromoles per square meter per second, 400-700 nanometers) for 20 hours was measured in leaves of annual crops differing widely in chilling tolerance. the incidence of photoinhibition, determined as the decrease in the ratio of induced to total chlorophyll fluorescence emission at 693 nanometers (f(v)/f(max)) measured at 77 kelvin, was not confined to chilling-sensitive species. the extent of photoinhi ...	1989	16666971
differences in the anaerobic lactate-succinate production and in the changes of cell sap ph for plants with high and low resistance to anoxia.	anaerobically treated seedlings of oryza sativa l. var arborio accumulated in their shoots more succinate than lactate and cell sap became alkaline. conversely, in triticum aestivum l. var mek 86 lactate accumulation was far higher than that of succinate and cell sap was acidified. anoxia clearly induced proton consumption in both species as an important means to prevent or counteract acidosis. other species studied were: echinochloa crus-galli l. beauv., zea mays l. var de kalb xl75, secale cer ...	1989	16666751
testing of genotoxic effects of 2,4-dichlorophenoxyacetic acid (2,4-d) using multiple genetic assay systems of plants.	the mutagenic activity of 2,4-d was assessed utilising cytogenetic, chlorophyll mutation, specific-locus and pollen viability endpoints in allium cepa and oryza sativa. in the allium root-tip assay onion bulbs with roots were treated with 25, 50, 75 or 100 ppm 2,4-d for 4 h and the frequency of aberrations was estimated. with an increase in concentration there was rise in frequency of aberrant cells. in the case of mutation assays, presoaked rice seeds were treated with 100, 200 or 300 ppm 2,4-d ...	1989	2761564
characterization of the rice (oryza sativa) actin gene family.	four rice (oryza sativa) genomic actin genes have been characterized. the rice actin genes show a conservation of intron number and position that is characteristic of plant actins. sequence comparisons revealed that the plant actins generally have a high degree of inter- and intraspecific sequence heterogeneity. however, one rice actin gene has a much higher degree of nucleotide sequence identity to a previously isolated actin sequence from arabidopsis thaliana than to any other plant actin gene ...	1990	2103449
interaction of elevated ultraviolet-b radiation and co(2) on productivity and photosynthetic characteristics in wheat, rice, and soybean.	wheat (triticum aestivum l. cv bannock), rice (oryza sativa l. cv ir-36), and soybean (glycine max [l.] merr cv essex) were grown in a factorial greenhouse experiment to determine if co(2)-induced increases in photosynthesis, biomass, and yield are modified by increases in ultraviolet (uv)-b radiation corresponding to stratospheric ozone depletion. the experimental conditions simulated were: (a) an increase in co(2) concentration from 350 to 650 microliters per liter; (b) an increase in uv-b rad ...	1990	16667735
localization of glyoxylate-cycle marker enzymes in peroxisomes of senescent leaves and green cotyledons.	crude particulate homogenates from leaves of barley (hordeum vulgare l.), rice (oryza sativa l.), leaf-beet (beta vulgaris var. cicla l.) and pumpkin (cucurbita pepo l.) cotyledons were separated on sucrose density gradients. the peroxisomal fractions appeared at a buoyant density of 1.25 g·cm(-3) and contained most of the activities of catalase (ec 1.11.1.6), and hydroxypyruvate reductase (ec 1.1.1.81) on the gradients. in peroxisomal fractions from detached leaves and green cotyledons incubate ...	1990	24202025
the genic nature of gamete eliminator in rice.	the two cultivated rice species, oryza sativa and oryza glaberrima, are morphologically alike but are reproductively isolated from each other by hybrid sterility. the hybrid is male sterile but partially female fertile. backcross experiments were conducted to introduce an alien factor controlling hybrid sterility from o. glaberrima (w025) into o. sativa (t65wx) and examine the genetic basis. an extracted sterility factor, closely linked to the wx locus, induced gametic abortion due to allelic in ...	1990	2341030
identification of two brassinosteroids from the cambial region of scots pine (pinus silverstris) by gas chromatography-mass spectrometry, after detection using a dwarf rice lamina inclination bioassay.	a simple and improved dwarf rice (oryza sativa var tan-ginbozu) lamina inclination bioassay for brassinosteroids (brs) was developed based on a previously published method (k takeno, rp pharis [1982] plant cell physiol 23: 1275-1281). the assay used 3-day-old intact seedlings, and detection of br was made more sensitive by synergizing the response to br with indole-3-acetic acid (iaa). the minimum detectable amount of br was less than 0.1 nanogram/rice plant (brassinolide equivalents). purificat ...	1990	16667906
nucleotide sequence of citrus limon 26s rrna gene and secondary structure model of its rna.	the complete nucleotide sequence of citrus limon 26s rdna has been determined. the sequence has been aligned with large ribosomal rna (l-rrna) sequences of escherichia coli, saccharomyces cerevisiae and oryza sativa. nine extensive expansion segments in dicot 26s rrna relative to e. coli 23s rrna have been identified and compared with analogous segments of monocot, yeast, amphibian and human l-rrnas. a secondary structure model for lemon 26s rrna has been derived based on the refined model of e. ...	1990	2101688
recognition efficiency of dicotyledoneae-specific promoter and rna processing signals in rice.	heterologous gene expression experiments have shown that genes of monocotyledoneae are often not transcribed in dicotyledoneae, or produce pre-mrna that is inefficiently or aberrantly processed. it is however not known how correctly and efficiently dicotyledon-specific gene expression signals are recognized in cells of monocotyledoneae. here we address this question using tobacco (nicotiana tabacum) and rice (oryza sativa) protoplasts transformed with the same hybrid gene constructs. constructs ...	1990	2177137
fingerprinting genomes using pcr with arbitrary primers.	simple and reproducible fingerprints of complex genomes can be generated using single arbitrarily chosen primers and the polymerase chain reaction (pcr). no prior sequence information is required. the method, arbitrarily primed pcr (ap-pcr), involves two cycles of low stringency amplification followed by pcr at higher stringency. we show that strains can be distinguished by comparing polymorphisms in genomic fingerprints. the generality of the method is demonstrated by application to twenty four ...	1990	2259619
food-based solutions are a viable alternative to glucose-electrolyte solutions for oral hydration in acute diarrhoea--studies in a rat model of secretory diarrhoea.	a survey of acute diarrhoea and its treatment, in 3 groups of villages in south india, revealed that use of the world health organization oral rehydration solution (who-ors) was poor or virtually non-existent and that several liquid foods were given to children during acute diarrhoea. the effects of the most commonly used, boiled and cooled supernatants of these liquid foods [rice (oryza sativa)-water, ragi (eleusine coracana)-water, arrowroot (maranta arundinacea)-water], and tender coconut-wat ...	1990	2345922
inheritance of tolerance to rice tungro bacilliform virus (rtbv) in rice (oryza sativa l.).	from a large number of rice varieties tested, no variety was identified as resistant to tungro bacilliform virus (rtbv). only in utri merah was the rtbv multiplication restrictive, whereas other varieties such as kataribhog and pankhari 203 were identified as tolerant. these varieties were crossed with a susceptible variety. tn1, to study the inheritance of restrictive multiplication and tolerance to rtbv. after 3 weeks of inoculation with rtbv, f1; f2, and f3 progenies were assessed by enzyme-l ...	1990	24221010
purification and partial characterization of pyruvate decarboxylase from oryza sativa l.	pyruvate decarboxylase(pyrdc) was purified from rice bran to a specific activity of 1 mu kat/mg and partially characterized. the holoenzyme is a tetramer of two types of subunits with molecular masses 64 kda and 62 kda. purified rice pyrdc exhibits positive cooperative kinetics with respect to pyruvate and functions with a significant lag phase. when compared to other plant pyrdc, the lag phase was shorter at low pyruvate concentrations and the s0.5 was smaller. the optimum ph (6.25) was also le ...	1990	2269301
introgression of genes from oryza officinalis well ex watt to cultivated rice, o. sativa l.	sterile ac hybrids between cultivated oryza sativa (aa) and a distant wild species, o. officinalis (cc), were backcross to o. sativa. most of the bc1 progenies were allotriploid (aac), a few were hypotriploid. aac progenies were again backcrossed to o. sativa. bc2 progenies consisting of disomic or aneuploid individuals were examined for the presence of o. officinalis traits. eleven different traits from o. officinalis were identified in these progenies. segregation data in the subsequent genera ...	1990	24221103
sucrose synthase in rice plants : growth-associated changes in tissue specific distributions.	different parts of the rice (oryza sativa l.) plant at different growth stages were analyzed for sucrose synthase (ss) by enzyme activity assay and enzyme-linked immunosorbent assay directly on the extracts or on the eluates from a gel filtration column. on a dry matter basis, the amount of soluble protein and ss activity decreased significantly, but the amount of enzyme protein changed little in growing leaves. in the grain, the ss activity was the highest at the early ripening stage and decrea ...	1990	16667853
proteins and amino acids of some local varieties of rice seeds (oryza sativa l.).	proteins and amino acids in four local rice (oryza sativa l.) varieties were identified. albumin and globulin were extracted from rice seeds, and the major promoters of these proteins were investigated by polyacrylamide gel electrophoresis to show their patterns. amino acid composition of the rice seed were determined quantitatively and qualitatively, and classified according to their acidic, basic and uncharged polar groups. essential amino acids for each variety were determined, and the hydrop ...	1990	2247436