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effect of some diets on macrolophus pygmaeus rambur (hemiptera: miridae) fitness under laboratory conditions.fitness parameters of omnivore predatory bug macrolophus pygmaeus were studied on various diets consisting of green peach aphid myzus persicae + eggplant leaf disc, eggs of ephestia kuehniella + eggplant leafdisc, pollen + eggplant leaf disc, eggs of e. kuehniella + pollen + eggplant leaf disc. the experiments were began from 1- day nymphs until 32-day adults. the tests were done at 25 degress c, 65+/-5% rh, 16l: 8d h photoperiod. the results showed that adding floral material to the animal prey ...200617385505
telomeric trans-silencing: an epigenetic repression combining rna silencing and heterochromatin formation.the study of p-element repression in drosophila melanogaster led to the discovery of the telomeric trans-silencing effect (tse), a repression mechanism by which a transposon or a transgene inserted in subtelomeric heterochromatin (telomeric associated sequence or tas) has the capacity to repress in trans in the female germline, a homologous transposon, or transgene located in euchromatin. tse shows variegation among egg chambers in ovaries when silencing is incomplete. here, we report that tse d ...200717941712
cytotype regulation by telomeric p elements in drosophila melanogaster: evidence for involvement of an rna interference gene.p elements inserted at the left telomere of the x chromosome evoke the p cytotype, a maternally inherited condition that regulates the p-element family in the drosophila germline. this regulation is completely disrupted in stocks heterozygous for mutations in aubergine, a gene whose protein product is involved in rna interference. however, cytotype is not disrupted in stocks heterozygous for mutations in two other rnai genes, piwi and homeless (spindle-e), or in a stock heterozygous for a mutati ...200717603126
unique germ-line organelle, nuage, functions to repress selfish genetic elements in drosophila melanogaster.the nuage is an electron-dense perinuclear structure that is known to be a hallmark of animal germ-line cells. although the conservation of the nuage throughout evolution accentuates its essentiality, its role(s) and the exact mechanism(s) by which it functions in the germ line still remain unknown. here, we report a nuage component, krimper (krimp), in drosophila melanogaster and show that it ensures the repression of the selfish genetic elements in the female germ line. the krimp loss-of-funct ...200717428915
[study on content and intake status of vegetable fiber in rural residents in china].to study the distribution and intake of vegetable fiber of chinese rural residents.200718303630
some biological features of cotton whitefly, bemisia tabaci (homoptera: aleyrodidae) on various host plants.development and reproduction of the cotton whitefly, bemisia tabaci (gennadius) were studied on aubergine, tomato and potato under laboratory conditions (30 degrees c and 55% rh). total life cycle from egg to adult was 14.9, 20.0 and 14.2 days on aubergine, tomato and potato, respectively. immature mortality were 12.9, 18.1 and 12.3% at the same three host plants. females of b. tabaci oviposited means of 51.8, 60.1 and 67.5 eggs on aubergine, tomato and potato, respectively and had a mean longev ...200719090122
zucchini and squash encode two putative nucleases required for rasirna production in the drosophila germline.rnai is a widespread mechanism by which organisms regulate gene expression and defend their genomes against viruses and transposable elements. here we report the identification of drosophila zucchini (zuc) and squash (squ), which function in germline rnai processes. zuc and squ contain domains with homologies to nucleases. mutant females are sterile and show dorsoventral patterning defects during oogenesis. in addition, oskar protein is ectopically expressed in early oocytes, where it is normall ...200717543859
interception of residual nitrate from a calcareous alluvial soil profile on the north china plain by deep-rooted crops: a 15n tracer study.15n-labeled nitrate was injected into different depths of an alluvial calcareous soil profile on the north china plain. subsequent movement of no3- -n and its recovery by deep-rooted maize (zea mays l.) and shallow-rooted eggplant (solanum melongena l.) were studied. under conventional water and nutrient management the mean recoveries of 15n-labeled nitrate from k(15)no3 injected at depths 15, 45, and 75 cm were 22.4, 13.8, and 7.8% by maize and 7.9, 4.9, and 2.7% by eggplant. the recovery rate ...200716979272
dissipation and residues of spinosad in eggplant and soil. 200717437051
detection of some safe plant-derived foods for ltp-allergic patients.lipid transfer protein (ltp) is a widely cross-reacting plant pan-allergen. adverse reactions to rosaceae, tree nuts, peanut, beer, maize, mustard, asparagus, grapes, mulberry, cabbage, dates, orange, fig, kiwi, lupine, fennel, celery, tomato, eggplant, lettuce, chestnut and pineapple have been recorded.200717505138
assessing effects of transgenic crops on soil microbial communities.deleterious effects of transgenic plants on soils represent an often expressed concern, which has catalyzed numerous studies in the recent past. in this literature review, studies addressing this question have been compiled. a total of 60 studies has been found, and their findings as well as their analytical approaches are summarized. these studies analyzed the effects of seven different types of genetically engineered traits, i.e., herbicide tolerance, insect resistance, virus resistance, prote ...200717522827
dietary risk assessment of spinosad in china.spinosad is an insect control agent that is derived from a naturally occurring soil bacterium, and is effective on several classes of insects, especially lepidoptera larvae. spinosad consists mainly of a mixture of spinosyns a and d and is registered for uses on a variety of crops in many countries including china. in order to understand the residue behavior of spinosad and to evaluate the dietary risk of spinosad residue in food in china, a number of residue studies were conducted on cotton, ch ...200717628261
ralstonia solanacearum strains from martinique (french west indies) exhibiting a new pathogenic potential.we investigated a destructive pathogenic variant of the plant pathogen ralstonia solanacearum that was consistently isolated in martinique (french west indies). since the 1960s, bacterial wilt of solanaceous crops in martinique has been caused primarily by strains of r. solanacearum that belong to either phylotype i or phylotype ii. since 1999, anthurium shade houses have been dramatically affected by uncharacterized phylotype ii strains that also affected a wide range of species, such as helico ...200717720825
evaluation of the weibull and log normal distribution functions as survival models of escherichia coli under isothermal and non isothermal conditions.published survival curves of escherichia coli in two growth media, with and without the presence of salt, at various temperatures and in a greek eggplant salad having various levels of essential oil, all had a characteristic downward concavity when plotted on semi logarithmic coordinates. some also exhibited what appeared as a 'shoulder' of considerable length. regardless of whether a shoulder was noticed, the survival pattern could be considered as a manifestation of an underlying unimodal dist ...200717869362
total phenolics and antioxidant capacity of indigenous vegetables in the southeast united states: alabama collaboration for cardiovascular equality project.collard greens, mustard greens, kale, okra, green onion, butter beans, butter peas, purple hull peas, rutabagas, and eggplant are frequently consumed by african americans in the southeast united states. sweet potato greens and purslane are two novel vegetables in this region. the objective of this study was to analyze total phenolics and antioxidant capacity in these indigenous vegetables. the total phenolic content was analyzed using the folin-ciocalteu method and ranged from 7.4 to 53.5 mg gal ...200717886092
strategy for allergenicity assessment of 'natural novel foods': clinical and molecular investigation of exotic vegetables (water spinach, hyacinth bean and ethiopian eggplant).foods not commonly consumed in the european union must be proven safe before being brought to market, including an assessment of allergenicity. we present a three-stepwise strategy for allergenicity assessment of natural novel foods using three novel vegetables, namely, water spinach, hyacinth bean, ethiopian eggplant.200717919138
an ecological study of lactic acid bacteria from almagro eggplant fermentation brines.identification of the predominant lactic acid bacteria (lab) involved in spontaneous fermentations of almagro eggplants, and evaluation of the biodiversity by molecular typing.200717953566
differential response related to genotoxicity between eggplant (solanum melanogena) skin aqueous extract and its main purified anthocyanin (delphinidin) in vivo.anthocyanins are the largest group of water-soluble pigments in the plant kingdom. a number of studies have demonstrated that anthocyanins present antioxidant capacity and show inhibitory effects on the growth of some cancer cells. thus, the goal of this study was to evaluate both the antimutagenicity/antigenotoxicity and mutagenicity/genotoxicity of aqueous extract obtained from the solanum melanogena, a possible novel source of anthocyanin, and its main purified anthocyanin extract (delphinidi ...200717194516
conservation and divergence of both phosphate- and mycorrhiza-regulated physiological responses and expression patterns of phosphate transporters in solanaceous species.here, orthologous genes of six phosphate transporter (pit) genes, which are members of the pht1 and pht2 families in tomato and potato, have been cloned from the solanaceous species pepper, eggplant and tobacco. overall, expressions of these genes in pepper, eggplant and tobacco showed similar patterns to those in tomato and potato: p-starvation enhancement in both leaves and roots for pht1;1, p-depletion induction exclusively in roots for pht1;2, mycorrhizal enhancement for pht1;3, and mycorrhi ...200717286830
isolation and characterisation of ralstonia solanacearum strains from solanaceae crops in ethiopia.eighty one isolates of ralstonia solanacearum -like bacteria on triphenyl tetrazolium chloride (ttc) medium were collected from different solanaceae crops (i.e. potato, tomato and pepper plants and potato tubers) at various sites in ethiopia. of these, 62 strains were identified as r. solanacearum based on their cultural characteristics on ttc medium, tomato pathogenicity bioassay, carbon source utilisation patterns and a specific pcr-based assay. by hayward's classification method, based on car ...200717304617
composition of arthropod species assemblages in bt-expressing and near isogenic eggplants in experimental fields.the environmental impact of genetically modified (gm) plants in experimental fields has been examined in several ways, in particular with respect to the dynamics of specific nontarget organisms. the approach of sampling for biodiversity in agroecosystems to compare complex patterns could also be useful in studying potential disruptions caused by gm crops. in this study, we set up replicated field plots of bt-expressing eggplants and near isogenic untransformed eggplants as a control. we monitore ...200717349136
dietary intake of carotenoid isomers in israel.controversy exists regarding the possible protective role of carotenoids against cancer. evidence is mainly against all-e-beta-carotene, while there is no evidence against other carotenoids or against mixtures of beta-carotene stereoisomers. carotenoid isomers could account for the variability in study results but are rarely estimated, and reference to the degree of their consumption is lacking. the aim of our study was to create a comprehensive database of carotenoid isomers content in food ite ...200718622950
molecular characterization and diagnosis of qoi resistance in cucumber and eggplant fungal pathogens.abstract the molecular mechanism of qoi fungicide resistance was studied using isolates of cucumber corynespora leaf spot fungus (corynespora cassiicola) and the eggplant leaf mold (mycovellosiella nattrassii). in both pathogens, a mutation at position 143 from glycine to alanine (g143a) was detected in the cytochrome b gene that encodes for the fungicide-targeted protein. moreover, the nucleotide sequence at amino acid position 143 was converted from ggt or gga in sensitive (wild-type) to gct o ...200718943516
diversity of ralstonia solanacearum infecting eggplant in the philippines.abstract the diversity of ralstonia solanacearum strains isolated from eggplant (solanum melongena) grown in five provinces of the philippine island group of luzon was assessed using a recently described hierarchical system. all strains keyed to race 1, biovar 3 or 4. phylotype-specific multiplex polymerase chain reaction (pcr) indicated that, like most other strains of asian origin, all the strains in our philippine collection belong to phylotype i. taxometric and phylogenetic analyses of parti ...200718943517
cutoff and aubergine mutations result in retrotransposon upregulation and checkpoint activation in drosophila.gametogenesis is a highly regulated process in all organisms. in drosophila, a meiotic checkpoint which monitors double-stranded dna breaks and involves drosophila atr and chk2 coordinates the meiotic cell cycle with signaling events that establish the axis of the egg and embryo. checkpoint activity regulates translation of the transforming growth-factor-alpha-like gurken signaling molecule which induces dorsal cell fates in the follicle cells [1-3]. we found that mutations in the drosophila gen ...200717363252
discrete small rna-generating loci as master regulators of transposon activity in drosophila.drosophila piwi-family proteins have been implicated in transposon control. here, we examine piwi-interacting rnas (pirnas) associated with each drosophila piwi protein and find that piwi and aubergine bind rnas that are predominantly antisense to transposons, whereas ago3 complexes contain predominantly sense pirnas. as in mammals, the majority of drosophila pirnas are derived from discrete genomic loci. these loci comprise mainly defective transposon sequences, and some have previously been id ...200717346786
a slicer-mediated mechanism for repeat-associated sirna 5' end formation in drosophila.in drosophila, repeat-associated small interfering rnas (rasirnas) are produced in the germ line by a dicer-independent pathway and function through the piwi subfamily of argonautes to ensure silencing of retrotransposons. we sequenced small rnas associated with the piwi subfamily member ago3. although other members of piwi, aubergine (aub) and piwi, associated with rasirnas derived mainly from the antisense strand of retrotransposons, ago3-associated rasirnas arose mainly from the sense strand. ...200717322028
drosophila rasirna pathway mutations disrupt embryonic axis specification through activation of an atr/chk2 dna damage response.small repeat-associated sirnas (rasirnas) mediate silencing of retrotransposons and the stellate locus. mutations in the drosophila rasirna pathway genes armitage and aubergine disrupt embryonic axis specification, triggering defects in microtubule polarization as well as asymmetric localization of mrna and protein determinants in the developing oocyte. mutations in the atr/chk2 dna damage signal transduction pathway dramatically suppress these axis specification defects, but do not restore retr ...200717199040
[effects of nacl stress on the growth, antioxidant enzyme activities and reactive oxygen metabolism of grafted eggplant].by the method of hydroponics and with the salt-tolerant eggplant cultivar 'torvum vigor' (solanum torvum) from japan as rootstock and the cultivar 'suqiqie' (solanum melongena l. ) as scion, this paper studied the differences between grafted and own-root seedlings in their photosynthetic characteristics, antioxidant enzyme activities, and reactive oxygen metabolism under 80 mmol x l(-1) nacl stress. the results showed that under nacl stress, the dry mass, chlorophyll content and photosynthetic r ...200717552189
production and characterization of arboreous and fertile solanum melongena + solanum marginatum somatic hybrid plants.in crop plants the shift from being annuals to perennials may allow future agricultural systems requiring less energy inputs. the practicability of this was tested for solanum melongena. leaf protoplasts of s. melongena (2n = 2x = 24) and one of the related arborescent species solanum marginatum (2n = 2x = 24) were electrofused and fertile somatic hybrids with arborescent habit regenerated. the magnetic cell sorter (macs) technique was used for the selection of heterokaryons. the hybrid nature o ...200717520277
[heat stress characteristics of photosystem ii in eggplant].with lower-and higher heat-resistant varieties of eggplant (solanum melongena l.) heibei i and heibei ii as test materials, and by using plant efficiency analyzer (pea) from hansatech, this paper measured the fast chlorophyll a fluorescence transient and its parameters. the results showed that ps ii construction became more sensitive to heat stress when ambient temperature was higher than 40 degrees c. the f0 went up slowly, and fv/fm and deltaf/fm' came down dramatically. heibei ii had a longer ...200717396501
potential of plant polyphenol oxidases in the decolorization and removal of textile and non-textile dyes.in this study an effort has been made to use plant polyphenol oxidases; potato (solanum tuberosum) and brinjal (solanum melongena), for the treatment of various important dyes used in textile and other industries. the ammonium sulphate fractionated enzyme preparations were used to treat a number of dyes under various experimental conditions. majority of the treated dyes were maximally decolorized at ph 3.0. some of the dyes were quickly decolorized whereas others were marginally decolorized. the ...200717915700
gene silencing mechanisms mediated by aubergine pirna complexes in drosophila male gonad.genetic studies have shown that aubergine (aub), one of the piwi subfamily of argonautes in drosophila, is essential for germ cell formation and maintaining fertility. aub mutations lead to the accumulation of retrotransposons in ovaries and testes, and stellate transcripts in testes. aub in ovaries associates with a variety of piwi-interacting rnas (pirnas) derived from repetitive intergenic elements including retrotransposons. here we found that aub in testes also associates with various kinds ...200717872506
in vitro studies of eggplant (solanum melongena) phenolics as inhibitors of key enzymes relevant for type 2 diabetes and hypertension.national diabetes education program of nih, mayo clinic and american diabetes association recommend eggplant-based diet as a choice for management of type 2 diabetes. the rationale for this suggestion is the high fiber and low soluble carbohydrate content of eggplant. we propose that a more physiologically relevant explanation lies in the phenolic-linked antioxidant activity and alpha-glucosidase inhibitory potential of eggplant which could reduce hyperglycemia-induced pathogenesis. results from ...200817706416
non-chromatographic speciation of toxic arsenic in vegetables by hydride generation-atomic fluorescence spectrometry after ultrasound-assisted extraction.a non-chromatographic, sensitive and simple analytical method has been developed for the determination of toxic arsenic species in vegetable samples by hydride generation-atomic fluorescence spectrometry (hg-afs). as(iii), as(v), dimethylarsinic acid (dma) and monomethylarsonic acid (mma) were determined by hydride generation-atomic fluorescence spectrometry using a series of proportional equations. the method is based on a single extraction of the arsenic species considered from vegetables thro ...200818585151
allergy to eggplant (solanum melongena) caused by a putative secondary metabolite.we describe a case of allergy caused by ingestion of eggplant in an atopic subject. symptoms included urticaria, itching of the throat, and hoarseness. skin prick test (spt) was positive with 4 varieties of eggplant; however, allergen-specific immunoglobulin e was not detected. spt with fractions of green long eggplant extract obtained by dialysis and ultrafiltration suggested the allergen to be less than 10 kd. spt following acetone precipitation of eggplant extract revealed that the allergen w ...200818361104
[cloning and expression analysis of drought-tolerant and wa-ter-saving related gene pmmyb in broomcorn millet].exploring novel genes and gene combinations are of primary importance in plant drought-tolerance and water-saving research. based on the est sequence of myb gene obtained in the research on molecular basis of drought-tolerance and water-saving in broomcorn millet (panicum miliaceum l.), a 1 739 bp genomic sequence of myb gene, designated pmmyb, was amplified using primers based on this est sequence and that of rice myb18. sequence analysis indicated that pmmyb consisted of 2 introns, 121 bp (347 ...200818332009
increment of iodine content in vegetable plants by applying iodized fertilizer and the residual characteristics of iodine in soil.as a new attempt to control iodine deficiency disorder (idd), we explored a method of iodine supplementation by raising the iodine content in vegetables. when grown in the soil supplemented with iodized fertilizer, the three experimental plant species (cucumber, aubergine, and radish) show increasing iodine levels in both leaf and fruit/rhizome tissues as the iodine content added in soil increases. excessive iodine added to soil can be toxic to plants, whereas the tolerance limit to excessive io ...200818265951
metabolism of conjugated sterols in eggplant. part 1. udp-glucose : sterol glucosyltransferase.a membrane-bound udp-glucose : sterol glucosyltransferase from solanum melongena (eggplant) leaves was partially purified and its specificity as well as molecular and kinetic properties were defined. among a wide spectrum of 3-oh steroids (i.e. typical plant sterols, androstane, pregnane and cholestane derivatives, steroidal alkaloids and sapogenins) and triterpenic alcohols, the highest activity was found with 22-oxycholesterol. udp-glucose appeared to be the best sugar donor. the enzyme prepar ...200818196184
metabolism of conjugated sterols in eggplant. part 2. phospholipid : steryl glucoside acyltransferase.a membrane-bound phospholipid : steryl glucoside acyltransferase from solanum melongena leaves was partially purified and its specificity and molecular as well as kinetic properties were defined. among the steryl glycosides tested (e.g. typical plant steryl glucosides, steryl galactosides and cholesteryl xyloside) the highest activity was found with cholesteryl glucoside, but some structurally related compounds such as sito- and stigmasteryl glucoside or galactoside as well as cholesteryl galact ...200818196183
chapter 16. how to define targets for small guide rnas in rna silencing: a biochemical approach.rna silencing involves various forms of sequence-specific gene silencing triggered by small rnas. in rna silencing, argonautes are crucial protein components that are directed to the target messenger rnas (mrnas) through their association with small rnas by base pairing. argonautes repress the expression of the target genes at posttranscriptional levels. full complementarity between a small rna and its target mrna results in argonaute-mediated cleavage ("slicing") of the target mrna. the d-d-h ( ...200819215766
structures and antioxidant activity of anthocyanins in many accessions of eggplant and its related species.anthocyanins were detected in extracts from the peels of 123 accessions of eggplant (solanum melongena) and its related species. their anthocyanin profiles were classified into four types, including known japanese eggplant type (type 1) and non-japanese eggplant type (type 2). although most of the eggplant accessions had one of the two known profiles, one accession had a novel profile (type 3). two accessions of related species showed another novel profile (type 4). the major anthocyanins were i ...200818831559
gene-based microsatellite development for mapping and phylogeny studies in eggplant.eggplant (solanum melongena l.) is a member of the solanaceae family. in spite of its widespread cultivation and nutritional and economic importance, its genome has not as yet been extensively investigated. few analyses have been carried out to determine the genetic diversity of eggplant at the dna level, and linkage relationships have not been well characterised. as for the other solanaceae crop species (potato, tomato and pepper), the level of intra-specific polymorphism appears to be rather l ...200818667065
heavy metal mobility in runoff water and absorption by eggplant fruits from sludge treated soil.sewage sludge addition to agricultural lands requires judicious management to avoid environmental risks arising from heavy metal and nitrate contamination of surface water and accumulation in edible plants. a field study was conducted on a silty-loam soil of 10% slope at kentucky state university research farm. eighteen plots of 22 x 3.7 m each were separated using metal borders and the soil in six plots was mixed with sewage sludge and yard waste compost mix (ss-yw) at 15 t acre(-1), six plots ...200818665990
a cross-sectional study on the prevalence of food allergy to eggplant (solanum melongena l.) reveals female predominance.only a few case reports of allergy to eggplant (solanum melongena) have been reported. a relatively large number of individuals appear to experience food-related symptoms to eggplant in india.200818681854
development of srap, srap-rga, rapd and scar markers linked with a fusarium wilt resistance gene in eggplant.fusarium wilt (fusarium oxysporum schlecht. f. sp. melongenae) is a vascular disease of eggplant (solanum melongena l.). the objectives of this work were (1) to confirm the monogenic inheritance of fusarium wilt resistance in eggplant, (2) to identify molecular markers linked to this resistance, and (3) to develop scar markers from most informative markers. we report the tagging of the gene for resistance to fusarium wilt (fom) in eggplant using srap, rga, srap-rga and rapd markers. analysis of ...200818712340
air and groundwater pollution in an agricultural region of the turkish mediterranean coast.air pollution and groundwater pollution in conjunction with agricultural activity were investigated in antayla province on the turkish mediterranean coast. the air pollution was investigated in terms of gas-phase nitric acid (hno3), sulfur dioxide (so2), ammonia (nh3), and particulate matter for a 6-month period in the atmosphere using a "filter pack" system, which was developed and optimized in our laboratory. ozone was measured by using an automated analyzer. among all of the gas-phase polluta ...200818817117
ancient chinese literature reveals pathways of eggplant domestication.changes in key traits occurring during the processes of plant domestication have long been subjects of debate. only in the case of genetic analysis or with extensive plant remains can specific sets of changes be documented. historical details of the plant domestication processes are rare and other evidence of morphological change can be difficult to obtain, especially for those vegetables that lack a substantial body of archaeological data. botanical records chronicled in the ancient literature ...200818819950
the hrp genes of pseudomonas cichorii are essential for pathogenicity on eggplant but not on lettuce.pseudomonas cichorii causes necrotic lesions in eggplant and rot in lettuce. through transposon insertion into p. cichorii strain spc9018 we produced two mutants, 4-57 and 2-99, that lost virulence on eggplant but not lettuce. analyses showed that a transposon was inserted into the hrpg gene in 4-57 and the hrct gene in 2-99. nucleotide sequences of the hrp genes of spc9018 are homologous to those of pseudomonas viridiflava bs group strains. the pathogenicity of 4-57 on eggplant was restored by ...200818832299
[nondestructive detection of grey mold of eggplant based on ground multi-spectral imaging sensor].botrytis cinerea pers. is a worldwide fungus. it is a severe threat to eggplant. chemistry methods can do an accurate identification, however they are time-consuming, require execution by professionals and are high cost. the present paper presents the development of a ground based multi-spectral imaging sensor for the grey mold detection. three channels (green, red, near-infrared) of crop images were acquired. two algorithm systems were developed. the objective of the image processing is to obta ...200818844147
evidence of local evolution of tomato-infecting begomovirus species in west africa: characterization of tomato leaf curl mali virus and tomato yellow leaf crumple virus from mali.tomato yellow leaf curl (tylc) and tomato leaf curl (tolc) diseases are serious constraints to tomato production in mali and other countries in west africa. in 2003 and 2004, samples of tomato showing virus-like symptoms were collected during a survey of tomato virus diseases in mali. three predominant symptom phenotypes were observed: (1) tylc/tolc (stunted upright growth and upcurled leaves with interveinal yellowing and vein purpling), (2) yellow leaf crumple and (3) broccoli or bonsai (sever ...200818278427
potential use of solanum melongena in agricultural areas with high mercury background concentrations.solanum melongena l. (eggplant) is a native culture from india, found throughout the world, and is one of the most popular vegetables in the mediterranean area. this work evaluates the potential use of eggplant as an economic alternative in an area with high mercury background concentrations. this implies the study of several factors such as the mercury absorption capacity of eggplant and the capacity of the soil for agricultural use. the research work has been carried out using soil from the me ...200818359138
aubergine gene overexpression in somatic tissues of aubergine(sting) mutants interferes with the rnai pathway of a yellow hairpin dsrna in drosophila melanogaster.aubergine (aub) is a member of the ppd family of proteins. these proteins are implicated in rna interference. in this article we demonstrate that the expression of the aub gene and protein increase in aub(sting) mutants. we used a genetic method to test whether aub(sting) overexpression could interfere with proper functioning of the process of rna interference in somatic tissues of drosophila melanogaster. this method is based on a transgenic line bearing a construct in which a fragment of the y ...200818385112
eco-friendly management of hadda beetle using beauveria bassiana in brinjal.brinjal (solanum melongena l.) is one among the vegetables grown in jaffna peninsula of sri lanka. it has been extensively cultivated throughout the country due to its demand among the consumers. the productivity of brinjal depends on the intensity of the attack of pests throughout the year. among the pests hadda beetle, epilachna vigintioctopunctata (coleoptera: coccinellidae) emerged as the key pest and caused serious damage on the foliage. this damage affected the flowering and fruiting abili ...200819226800
[alternative splicing of smu2af65 in solanum melongena l].u2af (u2 snrnp auxiliary factor), an essential auxiliary factor for pre-mrna splicing, is highly conserved across species. here we report the cloning and expression analysis of the homologous gene (smu2af65) encoding the large subunit of u2af65 in solanum melongena. gene specific primers for rapid amplification of cdna ends (race) are designed according to the genomic sequence of eggplant bac 77n19 and the full length cdna of tobacco npu2af65 a and b. the full length cdna of smu2af65 (genbank ac ...200819073560
toxicity of cadmium on the growth and yield of solanum melongena l.heavy metal cadmium is biomagnified through food chain and causes itai-itai disease in human. the present investigation reports the results of the effect of cadmium on seed germination, germination relative index (g.r.i.), seedling growth, chlorophyll stability index (csi) and yield of solanum melongena l. cv pusa uttam. effect of different concentrations of heavy metal cadmium (cdcl2) in hoagland's nutrient solution (10(-2) m, 10(-4) m, 10(-5) m and 10(-8) m) were employed for all seedling and ...200819297979
tomato (solanum lycopersicum): a model fruit-bearing crop.introductiontomato (solanum lycopersicum) is one of the most important vegetable plants in the world. it originated in western south america, and domestication is thought to have occurred in central america. because of its importance as food, tomato has been bred to improve productivity, fruit quality, and resistance to biotic and abiotic stresses. tomato has been widely used not only as food, but also as research material. the tomato plant has many interesting features such as fleshy fruit, a s ...200821356708
development of a real-time pcr method for the differential detection and quantification of four solanaceae in gmo analysis: potato (solanum tuberosum), tomato (solanum lycopersicum), eggplant (solanum melongena), and pepper (capsicum annuum).the labeling of products containing genetically modified organisms (gmo) is linked to their quantification since a threshold for the presence of fortuitous gmos in food has been established. this threshold is calculated from a combination of two absolute quantification values: one for the specific gmo target and the second for an endogenous reference gene specific to the taxon. thus, the development of reliable methods to quantify gmos using endogenous reference genes in complex matrixes such as ...200818303841
weed management in solanaceae crops in portugal.portugal has very good climatic-edafic conditions for solanaceae crops, regarding to either yield quality or quantity. tomato (lycopersicon esculentum miller.), potato (solanum tuberosum l.) and pepper (capsicum annuum l.) are the most social-economically important solanaceae and aubergine (solanum melongena l.) area of cultivation is increasing. tomato is cultivated for fresh consumption and, primarily, for industrial processing. is one of the most profitable vegetable crop and the main vegetab ...200819226844
[a case of food poisoning due to ingestion of eggplant, solanum melongena, grafted on devil's trumpet, datura metel].a husband and wife successively visited an emergency room with symptoms of staggering, slurred speech, mydriasis, and drowsiness, three hours after separately eating spaghetti with meat sauce. the sauce contained eggplant that had been grafted onto a devil's trumpet, datura metel. scopolamine and atropine were detected in the leftover sauce and in the sera of the patients. this is the first case of food poisoning related to datura in okinawa, japan and also might be the first report of food pois ...200819029791
agricultural reuse of reclaimed water and uptake of organic compounds: pilot study at mutah university wastewater treatment plant, jordan.the residues of polynuclear aromatic hydrocarbons (pahs), polychlorinated biphenyls (pcbs), chlorinated benzenes (cbs) and phenols were investigated for soil, wastewater, groundwater and plants. the uptake concentration of these compounds was comparatively determined using various plant types: zea mays l., helianthus annus l., capsicum annum l., abelmoschus esculentus l., solanum melongena l. and lycopersicon esculentum l. which were grown in a pilot site established at mutah university wastewat ...200818471853
does rna interference influence meiotic crossing over in drosophila melanogaster?mutations in the rna interference (rnai) genes aubergine (aub), homeless and piwi were tested for effects on the frequency, distribution and coincidence of meiotic crossovers in the long arm of the x chromosome. some increases in crossover frequency were seen in these tests, but they may have been due to a maternal effect of the balancer chromosomes that were used to maintain the rnai mutations in stocks rather than to the rnai mutations themselves. these same balancers produced strong zygotic i ...200818593512
issr and isozyme characterization of androgenetic dihaploids reveals tetrasomic inheritance in tetraploid somatic hybrids between solanum melongena and solanum aethiopicum group gilo.gene exchanges between solanum melongena and its allied relative solanum aethiopicum are a crucial prerequisite for introgression of useful traits from the allied species into the cultivated eggplant. in order to evaluate the extent of genetic recombination between the 2 species, biochemical and molecular markers were employed. a dihaploid population obtained through anther culture of the corresponding tetraploid somatic hybrids was genetically analyzed. the extent of disomic/tetrasomic inherita ...200818245797
host plant choice experiments of colorado potato beetle (coleoptera: chrysomelidae) in virginia.field and laboratory-choice experiments were conducted to understand aspects of host plant orientation by the colorado potato beetle, leptinotarsa decemlineata say (coleoptera: chrysomelidae), in virginia. in laboratory bioassays, l. decemlineata oriented to volatiles emitted by potato, solanum tuberosum l., foliage over both tomato, lycopersicon esculentum l., and eggplant, solanum melongena l., foliage, and eggplant over tomato foliage, all of which had been mechanically damaged. field choice ...200818613587
bioaccumulation of heavy metals and two organochlorine pesticides (ddt and bhc) in crops irrigated with secondary treated waste water.four crop plants oryza sativa (rice), solanum melongena (brinjal), spinacea oleracea (spinach) and raphanus sativus (radish) were grown to study the impact of secondary treated municipal waste water irrigation. these plants were grown in three plots each of 0.5 ha, and irrigated with secondary treated waste water from a sewage treatment plant. sludge from the same sewage treatment plant was applied as manure. cultivated plants were analyzed for accumulation of heavy metals and pesticides. result ...200918654831
differential growth response of various crop species to arbuscular mycorrhizal inoculation.to investigate the growth response of various crop species to mycorrhizal inoculation, arbuscular mycorrhizal fungi were applied to glycine max, vigna angularis, senna tora, hordeum vulgare var. hexastichon. zea mays, sorghum bicolor, allium tuberosum, solanum melongena, and capsicum annuum. the biomass of the inoculated crops was measured every two weeks for the 12-week growth period. by measuring biomass, we calculated the mycorrhizal responsiveness of the nine crop species. among the nine cro ...200923983512
ecological specialization of the aphid aphis gossypii glover on cultivated host plants.many plant-feeding insect species considered to be polyphagous are in fact composed of genetically differentiated sympatric populations that use different hosts and between which gene flow still exists. we studied the population genetic structure of the cotton-melon aphid aphis gossypii that is considered as one of the most polyphagous aphid species. we used eight microsatellites to analyse the genetic diversity of numerous samples of a. gossypii collected over several years at a large geographi ...200919635073
phytohormone-based activity mapping of insect herbivore-produced elicitors.in response to insect attack, many plants exhibit dynamic biochemical changes, resulting in the induced production of direct and indirect defenses. elicitors present in herbivore oral secretions are believed to positively regulate many inducible plant defenses; however, little is known about the specificity of elicitor recognition in plants. to investigate the phylogenic distribution of elicitor activity, we tested representatives from three different elicitor classes on the time course of defen ...200919124770
population dynamics of meloidogyne arenaria and pasteuria penetrans in a long-term crop rotation study.the endospore-forming bacterium pasteuria penetrans is an obligate parasite of root-knot nematodes (meloidogyne spp.). the primary objective of this study was to determine the effect of crop sequence on abundance of p. penetrans. the experiment was conducted from 2000 to 2008 at a field site naturally infested with both the bacterium and its host meloidogyne arenaria and included the following crop sequences: continuous peanut (arachis hypogaea) (p-p-p) and peanut rotated with either 2 years of ...200922736828
development of an improved isolation approach and simple sequence repeat markers to characterize phytophthora capsici populations in irrigation ponds in southern georgia.phytophthora capsici, the causal agent of phytophthora blight, is a major concern in vegetable production in georgia and many other states in the united states. contamination of irrigation water sources by p. capsici may be an important source of inoculum for the pathogen. a simple method was developed in this study to improve the efficiency of recovering p. capsici from fruits used as baits in irrigation ponds. in contrast to direct isolation on agar plates, infected fruit tissues were used to ...200919581483
the cucurbitaceae and solanaceae illustrated in medieval manuscripts known as the tacuinum sanitatis.beginning in the last two decades of the 14th century, richly illuminated versions of the tacuinum sanitatis, the latin translation of an 11th-century arabic manuscript known as taqwim al-sihha bi al-ashab al-sitta, were produced in northern italy. these illustrated manuscripts provide a window on late medieval life in that region by containing some 200 full-page illustrations, many of which vividly depict the harvest of vegetables, fruits, flowers, grains, aromatics and medicinal plants. our ob ...200919318382
cytotype regulation in drosophila melanogaster: synergism between telomeric and non-telomeric p elements.the x-linked telomeric p elements tp5 and tp6 interact synergistically with non-telomeric p elements to repress hybrid dysgenesis. in this repression, the telomeric p elements exert maternal effects, which, however, are not sufficient to establish synergism with the non-telomeric p elements. once synergism is established, the capacity to repress dysgenesis in the offspring of a cross persists for at least two generations after removing the telomeric p element from the genotype. at the molecular ...200920122295
pirnas mediate posttranscriptional retroelement silencing and localization to pi-bodies in the drosophila germline.nuage, a well-conserved perinuclear organelle found in germline cells, is thought to mediate retroelement repression in drosophila melanogaster by regulating the production of piwi-interacting rnas (pirnas). in this study, we present evidence that the nuage-pirna pathway components can be found in cytoplasmic foci that also contain retroelement transcripts, antisense pirnas, and proteins involved in messenger rna (mrna) degradation. these mrna degradation proteins, decapping protein 1/2 (dcp1/2) ...200919651888
peculiarities of pirna-mediated post-transcriptional silencing of stellate repeats in testes of drosophila melanogaster.silencing of stellate genes in drosophila melanogaster testes is caused by antisense pirnas produced as a result of transcription of homologous suppressor of stellate (su(ste)) repeats. mechanism of pirna-dependent stellate repression remains poorly understood. here, we show that deletion of su(ste) suppressors causes accumulation of spliced, but not nonspliced stellate transcripts both in the nucleus and cytoplasm, revealing post-transcriptional degradation of stellate rna as the predominant me ...200919321499
different aubergine alleles confirm the specificity of different rnai pathways in drosophila melanogaster.the crystal-stellate system is one of the best-known examples of heterochromatin-euchromatin interaction. the components of this system are homologous repetitive sequences clustered in three regions: 12e1 and h27 on the x and h11 on the y. the symptom of a disrupted crystal-stellate interaction is the presence of crystals in the spermatocytes of males lacking the crystal region. stellate silencing is based on the rnai process. many modifiers of this system have been isolated and many of these ar ...200919242123
qtl analysis of plant development and fruit traits in pepper and performance of selective phenotyping.a qtl analysis was performed to determine the genetic basis of 13 horticultural traits conditioning yield in pepper (capsicum annuum). the mapping population was a large population of 297 recombinant inbred lines (ril) originating from a cross between the large-fruited bell pepper cultivar 'yolo wonder' and the small-fruited chilli pepper 'criollo de morelos 334'. a total of 76 qtls were detected for 13 fruit and plant traits, grouped in 28 chromosome regions. these qtls explained together betwe ...200919219599
anthocyanins, colour and antioxidant properties of eggplant (solanum melongena l.) and violet pepper (capsicum annuum l.) peel extracts.acetone extracts from eggplant (solanum melongena l.) and violet pepper (capsicum annuum l.) peels both belonging to the solanaceae plant family were characterized with respect to their anthocyanin profiles, colour qualities and antioxidant capacities. according to hplc-dad-ms3 analyses the major anthocyanin in eggplant was delphinidin-3-rutinoside, while the predominant pigment in violet pepper was assigned to delphinidin-3-trans-coumaroylrutinoside-5-glucoside. since virtually all anthocyanins ...200916989312
life history and life tables of bactericera cockerelli (homoptera: psyllidae) on eggplant and bell pepper.the development, survivorship, and fecundity of the potato psyllid, bactericera cockerelli (sulc), fed on eggplant (solanum melongena l., variety special hibush) and bell pepper (capsicum annuum l., variety capsitrano) were studied in the laboratory at 26.7 +/- 2 degrees c, 70 +/- 5% rh, and at a photoperiod of 14:10 (l:d) h. immature b. cockerelli developed faster (24.1 d) when fed on eggplant than on bell pepper (26.2 d). survival rates of immature stages from egg to adult emergence were highe ...200920021762
[effects of host plants on selection behavior and biological parameters of bemisia tabaci gennadius biotype b].based on the observation of free diffusion in petri dish and biology, the selection behavior and biological parameters of bemisia tabaci gennadius biotype b on host plants cucumber, eggplant, pepper, cotton, and sweet potato were investigated. no difference was observed in the individual number of b. tabaci on the five host plant leaves during the early period of free diffusion (within 2 h), but changes were found within the following periods (4-48 h), i.e., the b. tabaci individuals increased o ...200920030150
teppeki, selective insecticide about bombus terrestris.at a time when a highly controversial debate about the causes of the widespread deaths of bees is taking place all over europe, which accused the agriculture and its practices with particular reference to the harmful effects of some insecticides, it seems important to point out as another insecticide, the teppeki, can be selective about bumble and have a good compatibility with the activity of the apiaries. this insecticide has the active ingredient flonicamid (500 g/kg) belonging to a new chemi ...200920222598
gene expression analysis in cadmium-stressed roots of a low cadmium-accumulating solanaceous plant, solanum torvum.solanum torvum sw. cv. torubamubiga (tb) is a low cadmium (cd)-accumulating plant. to elucidate the molecular mechanisms of the cd acclimation process in tb roots, transcriptional regulation was analysed in response to mild cd treatment: 0.1 mum cdcl(2) in hydroponic solution. a unigene set consisting of 6296 unigene sequences was constructed from 18 816 tb cdnas. the distribution of functional categories was similar to tomato, while 330 unigenes were suggested to be tb specific. for expression ...200919837731
risk assessment of thiacloprid and its chemical decontamination on eggplant, solanum melongena l.thiacloprid [(z)-3-(6-chloro-3-pyridylmethyl)-1,3-thiazolidin-2-ylidenecyanamide; calypso] is a systemic insecticide having persistence in the plant system. it was chosen for the management of the eggplant shoot and fruit borer, leucinodes orbonalis guen. management of this insect pest is difficult because it harbours inside the shoot and fruit portions of eggplant. the persistence of thiacloprid on eggplant has not been studied in india. the food and agriculture organisation (fao) has proposed ...200919089852
the potential of oil-utilizing bacterial consortia associated with legume root nodules for cleaning oily soils.the surfaces of root nodules of vicia faba and lupinus albus (legume crops), were colonized with bacterial consortia which utilized oil and fixed nitrogen. such combined activities apparently make those periphytic consortia efficient contributors to bioremediation of oily nitrogen-poor desert soils. this was confirmed experimentally in this study. thus, cultivating v. faba, l. albus and, for comparison, solanum melongena, a nonlegume crop, separately in oily sand samples resulted in more oil att ...200919103456
artificial substrates for oviposition and larval development of the pepper weevil (coleoptera: curculionidae).the pepper weevil, anthonomus eugenii cano (coleoptera: curculionidae), is a major pest of cultivated peppers (capsicum spp.) and other cultivated and wild species within the family solanaceae. laboratory study of this insect, as well as its biological control agents, will be greatly facilitated by an artificial rearing system that does not rely on pepper fruit. an egg collection method and amendments to a standard larval diet were investigated for use in the rearing of this weevil. spherical sa ...200919253644
arginine methylation of piwi proteins catalysed by dprmt5 is required for ago3 and aub stability.piwi family proteins are essential for germline development and bind piwi-interacting rnas (pirnas). the grandchildless gene aub of drosophila melanogaster encodes the pirna-binding protein aubergine (aub), which is essential for formation of primordial germ cells (pgcs). here we report that piwi family proteins of mouse, xenopus laevis and drosophila contain symmetrical dimethylarginines (sdmas). we found that piwi proteins are expressed in xenopus oocytes and we identified numerous xenopus pir ...200919377467
isolation and characterization of endophytic bacillus subtilis jaas ed1 antagonist of eggplant verticillium wilt.an endophytic bacterial strain, jaas ed1, was isolated from the interior of eggplant (solanum melongena l.) stems in jiangsu province, china. according to the morphological and physiological characteristics and phylogenetic analysis of the 16s rdna sequence, it was identified as bacillius subtilis. strain jaas ed1 and its cell-free filtrate had strong antifungal activity against verticillium dahliae. the strain was an internal colonizer within the eggplant without any harmful side effects to the ...200919584554
endophyte-induced verticillium protection in tomato is range-restricted.endophytes, bacterial, fungal or viral, colonize plants often without causing visible symptoms. more important, they may benefit host plants in many ways, most notably by preventing diseases caused by normally virulent pathogens. previous studies have shown that an isolate of v. dahliae from eggplant, dvd-e6, can colonize tomato endophytically, producing taller and more robust tomato plants while providing protection against a virulent v. dahliae, race 1 (vd1) isolate. expression analyses sugges ...200919649201
genetic similarity of eggplant shoot and fruit borer, leucinodes orbonalis, populations.shoot and fruit borer, leucinodes orbonalis (guenee) (pyraustidae: lepidoptera), has become a production constraint in all eggplant (solanum melongena linn. [solanaceae])-growing countries. in india, transgenic eggplants expressing bacillus thuringiensis cry toxins have been tested in fields by private- and public-sector agencies. understanding population diversity is important in designing strategies for better pest management. in the present investigation, random-amplified polymorphic dna mark ...200919715478
pepper weevil attraction to volatiles from host and nonhost plants.the location of wild and cultivated host plants by pepper weevil (anthonomus eugenii cano) may be aided by visual cues, the male-produced aggregation pheromone, herbivore-induced, or constitutive host plant volatiles. the attractiveness of constitutive plant volatiles to pioneer weevils is important in understanding, and perhaps controlling, dispersal of this insect between wild and cultivated hosts. ten-day-old male and 2- and 10-day-old female weevils were tested in short-range y-tube assays. ...200919791617
transgenic aubergines put on ice. 200919847236
small intestinal obstruction due to phytobezoar: a case report.patients with mechanical small-bowel obstructions usually present with abdominal pain, vomiting, absolute constipation and varying degrees of abdominal distention. causes can be classified as benign or malignant, or as extra- or intraluminal. a bezoar occurs most commonly in patients with impaired gastrointestinal motility. in edentulous older patients with abnormal food habits, it can also be an intestinal concretion that fails to pass along the alimentary canal. small bowel phytobezoars are ra ...200920062741
mutational analysis of eggplant latent viroid rna processing in chlamydomonas reinhardtii chloroplast.viroids of the family avsunviroidae, such as eggplant latent viroid (elvd), contain hammerhead ribozymes and replicate in the chloroplasts of the host plant through an rna-based symmetrical rolling-circle mechanism in which oligomeric rnas of both polarity are processed to monomeric linear rnas (by cleavage) and to monomeric circular rnas (by ligation). using an experimental system consisting of transplastomic lines of the alga chlamydomonas reinhardtii, a mutational analysis of sequence and str ...200919675190
analysis of the stable free radical scavenging capability of artificial polyphenol mixtures and plant extracts by capillary electrophoresis and liquid chromatography-diode array detection-tandem mass spectrometry.the oxidation process of phenolic compounds of an artificial mixture consisting of six polyphenols and the extract of eggplant (solanum melongena) skin was monitored by using capillary zone electrophoresis and liquid chromatography-diode array detection-tandem mass spectrometry. the methods developed enabled simultaneous evaluation of the antioxidative capability of each compound. the above oxidation process was carried out using two radicals, viz. the 2,2-diphenyl-1-picrylhydrazyl and hydroxyl ...200919185871
higher histamine sensitivity in non-atopic subjects by skin prick test may result in misdiagnosis of eggplant allergy.eggplant (aubergine; solanum melongena l.), a member of the nightshade family, is widely consumed as a vegetable. a high incidence of allergic reactions to eggplant appears to be commonly experienced among indians, presumably due to its high histamine content. therefore, it appeared interesting to investigate the histamine content of different varieties of eggplant (green and purple round; green and purple slender long) using raw, cooked, and dialyzed extracts. a significant varietal difference ...200919172488
collapse of germline pirnas in the absence of argonaute3 reveals somatic pirnas in flies.piwi-interacting rnas (pirnas) silence transposons in animal germ cells. pirnas are thought to derive from long transcripts spanning transposon-rich genomic loci and to direct an autoamplification loop in which an antisense pirna, bound to aubergine or piwi protein, triggers production of a sense pirna bound to the piwi protein argonaute3 (ago3). in turn, the new pirna is envisioned to produce a second antisense pirna. here, we describe strong loss-of-function mutations in ago3, allowing a direc ...200919395009
application of indoxacarb for managing shoot and fruit borer of eggplant (solanum melongena l.) and its decontamination.indoxacarb was applied at 75 and 150 g a.i. ha(-1) for two years to an eggplant (solanum melongena l.) crop grown in the field plots in order to evaluate its efficacy for management of the lepidopteron pest, shoot and fruit borer. the residues of the insecticide were quantified by high-pressure liquid chromatography (hplc). the mean initial deposits of indoxacarb on eggplant fruits were found to be 2.60-2.634 mg kg(-1) and 3.64-3.68 mg kg(-1) from the two rates of applications, respectively. the ...200919280483
effect of domestic cooking methods on the total antioxidant capacity of vegetables.as well as tables of food composition, total antioxidant capacity (tac) databases--developed in past years for investigating health effects of antioxidant-rich foods in epidemiological and nutritional studies--have been created on the basis of analytical data from raw plant material. however, most vegetables are commonly cooked before consumption. with the aim of completing a previously developed tac database, the effect of the major domestic practices (i.e. boiling, pan-frying and deep-frying) ...200919255918
functional involvement of tudor and dprmt5 in the pirna processing pathway in drosophila germlines.in drosophila, the piwi proteins, aubergine (aub), ago3, and piwi are expressed in germlines and function in silencing transposons by associating with piwi-interacting rnas (pirnas). recent studies show that piwi proteins contain symmetric dimethyl-arginines (sdmas) and that dprmt5/capsuleen/dart5 is the modifying enzyme. here, we show that tudor (tud), one of tud domain-containing proteins, associates with aub and ago3, specifically through their sdma modifications and that these three proteins ...200919959991
a regulatory circuit for piwi by the large maf gene traffic jam in drosophila.piwi-interacting rnas (pirnas) silence retrotransposons in drosophila germ lines by associating with the piwi proteins argonaute 3 (ago3), aubergine (aub) and piwi. pirnas in drosophila are produced from intergenic repetitive genes and pirna clusters by two systems: the primary processing pathway and the amplification loop. the amplification loop occurs in a dicer-independent, piwi-slicer-dependent manner. however, primary pirna processing remains elusive. here we analysed pirna processing in a ...200919812547
clinico-immunological analysis of eggplant (solanum melongena) allergy indicates preponderance of allergens in the peel.: eggplant (solanum melongena l.) is known to cause food allergy in some asian countries but detailed studies on eggplant allergy are lacking.200923283148
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