Publications

the biosynthesis of sulfoquinovosyldiacylglycerol: studies with groundnut (arachis hypogaea) leaves.	the biosynthetic pathway of sulfoquinovosyldiacylglycerol (sqdg) was investigated using groundnut (arachis hypogaea) leaf discs and 35s-labeled precursors. [35s]so4(2-) was actively taken up by the leaf discs and rapidly incorporated into sqdg. after 2 h, 1.5% of the [35s]so4(2-) added to the incubation medium was taken up, of which 28% was incorporated into sqdg. the methanol-water phases of the lipid extracts of the leaf discs were analyzed for the 35s-labeled intermediates. up to 2 h of incub ...	1988	3341737
models relating subcellular effects of temperature to whole plant responses.	the analysis of the temperature dependence of the rate of photosynthetic carbon assimilation by leaves of c3 plants has involved subdivision into a diffusional (mainly stomatal) component and a biochemical one. the latter has been further analysed in terms of rubisco activity and of the capacity for regeneration of rubp. rubisco activity has been modelled in terms of the number of active (carbamylated) enzyme sites and the kinetics of these sites. rubp regeneration capacity has been modelled in ...	1988	3152040
lectin histochemistry of the thyroid gland.	the authors carried out a histochemical study with lectins (ulex europaeus agglutinin-i [uea-i], triticum vulgaris [wga], glycine max [sba], dolichos biflorus [dba], and arachis hypogaea [pna]) in different thyroid gland conditions (17 benign nodular goiters, three diffuse hyperplasias, five hashimoto's thyroiditis, 20 follicular adenomas, 14 well-differentiated papillary carcinomas, five well-differentiated follicular carcinomas, and 30 normal thyroids) in order to determine if specific lectin ...	1988	3179951
differences in properties of peanut seed lectin and purified galactose- and mannose-binding lectins from nodules of peanut.	two lectins were purified by affinity chromatography from mature peanut (arachis hypogaea l.) nodules, and compared with the previously characterised seed lectin of this plant. one of the nodule lectins was similar to the seed lectin in its molecular weight and amino-acid composition and ability to bind derivatives of galactose. however, unlike the seed lectin, this nodule lectin appeared to be a glycoprotein and the two lectins were only partially identical in their reaction with antibodies pre ...	1988	24220730
detection of lectins in nodulated peanut and soybean plants.	the direct double-antibody enzymelinked immunosorbent assay system was used in the detection and measurement of seed lectins from peanut (arachis hypogaea l.) and soybean (glycine max l.) plants (psl and sbl, respectively) that had been inoculated with their respective rhizobia. concentrations of psl dropped to undetectable levels in peanut roots at 9 d and stems and leaves at 27 d after planting; sbl could no longer be detected in soybean roots at 9 d and in stems and leaves at 12 d. a lectin a ...	1988	24220729
bahiagrass for the management of meloidogyne arenaria in peanut.	bahiagrass (paspalum notatum) cultivars argentine, pensacola, and tifton-9 were non-hosts for meloidogyne arenaria, m. incognita, and heterodera glycines in a greenhouse experiment using field soil infested with these nematodes. the effect of pensacola bahiagrass in rotation with peanut (arachis hypogaea) on m. arenaria was studied in 1986 and 1987 in a field at the wiregrass substation near headland, alabama. each year soil densities of second-stage juveniles of m. arenaria, determined near pea ...	1988	19290315
potential of crops uncommon to alabama for management of root-knot and soybean cyst nematodes.	vigna unguiculata, cassia fasiculata, and sesamum indicum did not support meloidogyne arenaria, m. incognita, or heterodera glycines race 4 in greenhouse studies with soils from peanut and soybean fields. fagopyron eseulentum, cyamopsis tetragonoloba, and cucurbita pepo were hosts to the two meloidogyne spp. but were nonhosts to h. glycines. meloidogyne arenaria and m. incognita galled but reproduced poorly in the roots of three types of amaranthus cruentus, and low densities of these two meloid ...	1988	19290317
fragmentation of the beta-subunit of human chorionic gonadotropin produced by choriocarcinoma.	when human chorionic gonadotropin (hcg) was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions with dithiothreitol (dtt), a smaller weight material (ctp'), in addition to the beta-subunit, could be detected by western blot analysis using antiserum for hcg beta-carboxy-terminal peptide (ctp). the ctp' band was much more apparent with urinary hcg from a patient with choriocarcinoma than with that from normal pregnant women. second-dimensional electroph ...	1988	2454807
purification and characterization of a differentiation-specific sialoglycoprotein of lactating-guinea-pig mammary tissue.	a large acidic glycoprotein, pas-i, was purified from the fat-globule membrane of guinea-pig milk. threonine and serine accounted for over 30 mol% of the amino acids, and galactose, n-acetylgalactosamine, n-acetylglucosamine, mannose and sialic acid were the principal sugars detected. on a molar basis, sialic acid accounted for over 60% of the total sugar. removal of sialic acid by treatment with neuraminidase revealed the presence of binding sites for peanut (arachis hypogaea) agglutinin, a lec ...	1988	2456756
glycoconjugates in normal human kidney. a histochemical study using 13 biotinylated lectins.	the avidin-biotin-peroxidase complex technique was used with 13 lectins to study the glycoconjugates of normal human renal tissue. the evaluated lectins included triticum vulgaris (wga), concanavalin ensiformis (cona), phaseolus vulgaris leukoagglutinin and erythroagglutinin (pha-l and pha-e), lens culinaris (lca), pisum sativum (psa), dolichos biflorus (dba), glycine max (sba), arachis hypogaea (pna), sophora japonica (sja), bandeiraea simplicifolia i (bsl-i), ulex europaeus i (uea-i) and ricin ...	1988	2466021
cytochemical approach to the development and behaviour of rat alveolar type ii cells in culture.	the development and characteristics of rat alveolar type ii cells were monitored by using various cytochemical techniques. polarized light microscopy was found useful for observing live type ii cells in culture. cells progressively lose their birefringent granules starting from 48 h of cells in culture, indicating the disappearance of the phospholipids organized lamellae in the lamellar bodies. similar results were obtained by using an immunocytochemical approach with antibodies raised against t ...	1988	2450083
method for showing human spermatogenesis using arachis hypogaea (aha) lectin.	using biotinylated arachis hypogaea agglutinin (aha) or peanut lectin (pna) and an avidin-peroxidase procedure, the various stages of spermatid development were visualised with great clarity; a light haematoxylin counterstain permitted the easy recognition of spermatogenic cells in the same section. this method is particularly useful in the interpretation of poorly fixed material and may be helpful in studies of cyclical maturation of spermatozoa, irrespective of whether the material is obtained ...	1988	3056983
histogenesis of the renal cysts in adult (autosomal dominant) polycystic kidney disease: a histochemical study.	we studied the kidneys from ten patients with adult (autosomal dominant) polycystic kidney disease (apkd) stained with lectins specific for different segments of the nephron on 20 cysts from each case (ranging in size from 0.1 to 1.3 cm in nine cases and from 1.5 to 6 cm in one case). the epithelium of all cysts with positive reactivity (arachis hypogaea and epithelial membrane antigen) was of collecting duct origin. many cysts remained unstained. cysts of proximal tubule origin could not be ide ...	1988	3065782
cell type-specific glycoconjugates of collecting duct cells during maturation of the rat kidney.	the ontogeny of lectin-positive epithelial cell types and the maturation of polarized expression of the glycocalyx of the collecting ducts (cd) of the rat kidney were studied from samples of 18th-day fetal and neonatal kidneys of various ages. lectins from dolichos biflorus (dba) and vicia villosa (vva), with preferential affinity to principal cells, stained virtually all cd cells of the fetal kidneys. however, within two days postnatally, the number of cells positive for dba and vva decreased t ...	1988	2842054
heterogeneity of apical glycoconjugates in kidney collecting ducts: further studies using simultaneous detection of lectin binding sites and immunocytochemical detection of key transport enzymes.	in the search for a functional role for the polarized glycoconjugates of rat collecting duct epithelial cells, the relation between binding of various lectins and expression of cellular transport enzyme profile of the cells was studied. for this purpose, principal and intercalated cells of rat kidney collecting duct were identified by morphological criteria and by their immunocytochemically determined content of (na+ + k+)-atpase and carbonic anhydrase (ca ii), respectively. various n-acetylgala ...	1988	2853698
lectin binding to the human retina.	formalin-fixed, paraffin-embedded tissue sections of the human retina were stained with different fluorescein isothiocyanate-conjugated lectins. the lectins used were concanavalin a (con a), triticum vulgaris (wga), glycine maximum (sba), dolichos biflorus (dba), ulex europaeus (uea i), arachis hypogaea (pna), and ricinus communis (rca i). con a stained both the inner and outer segments of the rods and cones, whereas wga stained the inner and outer segments of the rods and the outer segments of ...	1988	3128141
pneumocystis carinii: surface reactive carbohydrates detected by lectin probes.	pneumocystis carinii obtained from rat lungs (rlh) and in vitro culture (rtc) were reacted with a panel of 14 fluorescein isothiocyanate conjugated lectins. percentage fluorescence and fluorescent intensity were determined for both trophic and cyst forms. all rlh and rtc derived organisms bound strongly concanavalin a (con a), and wheat germ agglutinin (wga). however, differences in soybean agglutinin (sba) binding between rlh and rtc organisms was observed. different subsets of the organism bou ...	1988	3142787
surface carbohydrate changes on onchocerca lienalis larvae as they develop from microfilariae to the infective third-stage in simulium ornatum.	use was made of seven fitc labelled lectins as tools to investigate the surface of onchocerca lienalis larvae as they develop through to the infective third-stage in a natural vector, simulium ornatum. the lectins were derived from canavalia ensiformis (con a), lens culinaris (lentil), triticum vulgaris (wheat germ), arachis hypogaea (peanut), helix pomatia, phaseolus vulgaris (kidney bean) and tetragonolobus purpureus (asparagus pea). between 70 and 100 living parasites were examined for each d ...	1988	3142948
endogenous as well as exogenous hormonal modulation of lectin binding sites in normal and neoplastic tissue of rat mammary gland.	free and sialic acid conjugated binding sites for the lectin from arachis hypogaea (peanut agglutinin, pna) have been histochemically demonstrated in normal rat mammary tissue and in n-nitrosomethylurea (nmu) induced mammary tumors of rats. the lectin binding sites were predominantly associated with secretory activities of the normal and neoplastic breast tissue (secretory pna binding sites). in normal breast tissue and in nmu induced mammary tumors the expression of secretory pna binding sites ...	1988	3150553
the toxicity of indian calotropis procera rbr latex in the black rat, rattus rattus linn.	toxicity studies with latex of calotropis procera rbr were conducted in the black rat, rattus rattus. the latex was fed through bait prepared from wheat flour, ground nut oil and sugar at concentrations of 5, 7.5 or 10% (w/w). the bait was fed up to mortality and for a maximum of 10 days. the signs produced were passivity, sluggishness, sedation, dyspnea, weakness, reduction in weight, anorexia, diarhhea, hematuria, bleeding from nose, eyes and anus, eye lens opacity, mild tetanic convulsions, c ...	1988	3176304
changes in peanut lectin binding sites on the neuroectoderm during neural tube formation in the bantam chick embryo.	cell surface carbohydrates in the neurulating ectoderm of bantam chick embryos of stage 6-11 were examined using the fluorescein isothiocyanate-labeled and ferritin-labeled peanut lectin, arachis hypogaea agglutinin (pna), which is gal beta 1----3galnac specific. weak fluorescence showing pna binding sites was seen on the apical surfaces of neural plate cells. on the surfaces of neural tube cells the fluorescence was more intense and appeared as a band. when using ferritin particles as a quantit ...	1988	3177890
specific and nonspecific inhibition of adhesion of oral actinomyces and streptococci to erythrocytes and polystyrene by caseinoglycopeptide derivatives.	various caseinoglycopeptide derivatives prepared from mammalian milk were evaluated as inhibitors of hemagglutinations mediated by actinomyces viscosus ny1, streptococcus sanguis omz9, and, for comparative purposes, plant lectins from arachis hypogaea and bauhinia purpurea. it was found that recognition of the beta-d-galactose-(1----3)-2-acetamido-2-deoxy-d-galactose carbohydrate chain by actinomyces viscosus ny1 organisms and arachis hypogaea and b. purpurea agglutinins had similar structural r ...	1988	3182077
tracheobronchial epithelium of the sheep: iv. lectin histochemical characterization of secretory epithelial cells.	conventional histochemical characterization of the mucus secretory apparatus is often difficult to reconcile with the biochemical analysis of respiratory secretions. this study was designed to examine the secretory glycoconjugates in airways using lectins with biochemically defined affinities for main sugar residues of mucus. we used five biotinylated lectins--dba (dolichos biflorus) and sba (glycine max) for n-acetyl galactosamine (galnac), bsa i (bandeiraea simplicifolia) and pna (arachis hypo ...	1988	3189886
mycoflora and mycotoxins of peanut (arachis hypogaea l.) seeds in egypt. iii. cellulose-decomposing and mycotoxin-producing fungi.	from 40 peanut seed samples collected in egypt, forty-three species and one variety of fungi, belonging to 16 genera, were collected. the most dominant genera were aspergillus (11 species + one variety), penicillium (11 species) and fusarium (4 species). from the preceding genera a. fumigatus, a. flavus, a. niger, p. chrysogenum and f. oxysporum were the most frequent species. forty-nine isolates belonging to 12 species and one variety were tested for production of mycotoxins, after growth on li ...	1988	3216881
expression of glycoconjugates on normally developing and immunologically impaired hymenolepis diminuta.	the carbohydrates on the surface of hymenolepis diminuta were analyzed with gold-labelled lectins, and it was found that the surface coat of the anterior body differs from that of the strobila in its lectin-binding properties. binding sites for lectins from abrus precatorius (apa), arachis hypogaea (pna), glycine max (sba) and for wheat germ agglutinin (wga) and succinylated wga were located on the scolex and strobilation zone. lectin-gold particles attached mainly to the electron-dense spines. ...	1988	3237676
lectin histochemistry on mucous substances of the taste buds and adjacent epithelia of different vertebrates.	in the present study carbohydrate residues in taste buds (tbs) and adjacent epithelial formations of a teleostean fish, a frog and the rabbit were detected by means of lectin histochemistry. biotinylated lectins from pisum sativum (psa), arachis hypogaea (pna), dolichos biflorus (dba), triticum vulgaris (wga and succinylated wga), glycine max (sba) and ulex europaeus (uea i) have been applied. the lectins were bound to an avidin-biotin-peroxidase-complex (abc) and visualized by diaminobenzidine/ ...	1988	3259218
carbohydrate-binding specificities of five lectins that bind to o-glycosyl-linked carbohydrate chains. quantitative analysis by frontal-affinity chromatography.	the carbohydrate-binding specificities of lectins purified from agaricus bisporus (aba-i), arachis hypogaea (pna), bauhinia purpurea (bpa), glycine max (sba), and vicia villosa (vva-b4) have been studied by affinity chromatography on columns of the immobilized lectins, and quantitatively analyzed by frontal affinity chromatography. these five lectins could be classified into two groups with respect to their reactivities with typical mucin-type glycopeptides, beta-d-galp-(1----3)-alpha-d-galpnac- ...	1988	3274131
site-specific monoclonal antibodies against peanut agglutinin (pna) from arachis hypogaea. immunohistochemical study of tissue-cultured cells and of 27 cases of hodgkin's disease.	the purpose of this study was to increase the sensitivity of the staining reaction for the t antigen on the surface of neoplastic cells grown in vitro with the use of site-specific monoclonal antibodies (mabs). the authors describe anti-peanut agglutinin (pna) mabs selected by screening the hybridomas with pna and pna bound to bovine serum albumin conjugated with the t antigen. the selected hybridomas (f2c8, f3d12, f3a5) were then grown in pristane-sensitized mice or in the amicon hollow fiber s ...	1988	3282447
comparative study on the main membrane-surface sialoglycopeptides released from young and old human erythrocytes with trypsin.	1. the amount of sialoglycopeptide (sgp) mixture released from young (y) erythrocytes by trypsin treatment was significantly larger than that from old (o) ones. 2. the two main membrane-surface sialoglycopeptides (y-sgp and o-sgp) were isolated in yields of about 45% and 42% from the mixtures by a combination of sephadex g-50 superfine gel filtration with deae-cellulose chromatography, respectively. molecular weights of 12,000 and 12,100, respectively, were found for the y-sgp and the o-sgp. 3. ...	1988	3356136
haemoglobin m-hyde park associated with polyagglutinable red blood cells in a south african family.	twelve of 35 members tested in a large ethnically-mixed south african family were found to have both haemoglobin m type hyde park and persistent polyagglutinable red blood cells. the characteristics of the polyagglutination have not been recorded previously. the cells of affected family members were not agglutinated by arachis hypogea, dolichos biflorus or salvia sclarea, but were agglutinated weakly by salvia horminum and bsii (gsii) and reacted strongly with glycine soja and sophora japonica l ...	1988	3377987
glycoconjugate expression in human renal oncocytomas. a lectin histochemical study.	a panel of lectins chosen for its specificity for segments of the nephron was used in an avidin-biotin-peroxidase reaction to stain 13 human renal oncocytomas and a specimen of oncocytomatosis. the lectins were derived from lotus tetragonolobus, glycine max, dolichos biflorus, and arachis hypogaea. two patterns predominated: one of staining with the lectins of d biflorus and g max, suggesting distal tubular or collecting-duct differentiation, and one of staining with the lectin of l tetragonolob ...	1988	3395218
efficacy of fumigant and nonfurmigant nematicides for control of meloidogyne arenaria on peanut.	three tests were conducted to evaluate the efficacy of fumigant and nonfumigant nematicides for control of meloidogyne arenaria race 1 on peanut. methyl bromide, 1,3-d, methyl isothiocyanate, and methyl isothiocyanate mixtures were applied 7 or 8 days preplant either broadcast or in-the-row. aldicarb, ethoprop, fenamiphos, and f5145 were applied at different rates and by different methods at-plant or at early flowering. of the 32 treatments evaluated, only seven resulted in yield increases (p = ...	1988	19290312
soybean-peanut rotations for the management of meloidogyne arenaria.	rotating soybean (glycine max cv. kirby) with peanut (arachis hypogaea cv. florunner) for managing meloidogyne arenaria race 1 was studied for 3 years (1985-87) in a field near headland, alabama. each year soybean plots had lower soil numbers of m. arenaria second-stage juveniles (j2) at peanut harvest than did plots in peanut monocnlture. peanut following either 1 or 2 years of soybean resulted in approximately 50% reduction in j2 soil population densities and a 14% (1-year soybean) or 20% (2-y ...	1988	19290309
crops uncommon to alabama for the management of meloidogyne arenaria in peanut.	in a 1987 field study juveniles of meloidogyne arenaria assayed at the time of peanut harvest were almost undetectable in plots planted with american jointvetch (aeschynomene americana), castor bean (ricinus communis), partridge pea (cassia fasiculata), sesame (sesamum indicum), and cotton (gossypium hirsutum), whereas plots with peanut (arachis hypogaea) averaged 120 juveniles/100 cm(3) soil. application of aldicarb in peanut resulted in an average of 27 juveniles/100 cm(3) soil. in 1988 all pl ...	1989	19287678
effects of bahiagrass and nematicides on meloidogyne arenaria on peanut.	a field infested with meloidogyne arenaria and with a history of peanut yield losses was divided into two equal parts. one-half of the field (bahia site) was planted to bahiagrass in 1986 and maintained through 1987. the other half (peanut site) was planted to soybean in 1986 and peanut in 1987 with hairy vetch planted each fall as a cover crop. in 1988 identical nematicide treatments including 1,3-dichloropropene (1,3-d), aldicarb, and ethoprop were applied to the two sites, and the sites were ...	1989	19287670
resistance to meloidogyne arenaria in arachis spp. germplasm.	field and greenhouse evaluations of 116 wild arachis spp. genotypes demonstrated the presence of resistance to reproduction of the root-knot nematode meloidogyne arenaria race 1. resistance in greenhouse tests was based on test lines having </= 2.5% of the number of eggs per gram of roots as did the susceptible a. hypogaea cv. tamnut 74. in field tests, resistant genotypes were identified on the basis of having lower (p = 0.05) final nematode population densities than did tamnut 74. resistance w ...	1989	19287667
ditylenchus destructor in hulls and seeds of peanut.	the potato rot nematode, ditylenchus destructor thorne, is reported for the first time in hulls and seeds of peanut. the populations found differed from d. dipsaei and d. myceliophagus in habitat, number of lateral incisures, shape of tail tip, and length of postvulval sac. infected hulls had brown necrotic tissue at the point of connection with the peg, and a black discoloration appeared first along the longitudinal veins. infected seeds were usually shrunken, and testae and embryos had a yello ...	1989	19287571
lectin histochemistry of the embryonic heart: expression of terminal and penultimate galactose residues in developing rats and chicks.	rat embryos at days 10-18 of gestation and chicken embryos at days 3-6 of incubation were fixed and processed for lectin histochemistry. the distribution of binding sites for a lectin from the peanut arachis hypogaea (pna) conjugated to horseradish peroxidase (hrp) was determined on tissue sections both before and after enzymatic cleavage of sialic acid with neuraminidase (sialidase). endocardial cushion tissue in the rat, but not in the chick, reacted with pna-hrp prior to digestion with sialid ...	1989	2916442
cytochemical localization of carbohydrates in intercalated duct and acinar cells of mouse parotid gland.	the glycoconjugate composition of mouse intercalated duct and acinar cells of parotid gland has been compared. mucins containing 1,2-glycols were demonstrated by the tannic acid-uranyl acetate technique. hexose residues of glycoconjugates were identified using ferritin conjugated with canavalia ensiformis agglutinin (con a), triticum vulgare or wheat germ agglutinin (wga), ricinus communis i agglutinin (rca-i), phaseolus vulgaris agglutinin (pha-e) and arachis hypogaea agglutinin (pna). whereas ...	1989	2470700
isolation and partial characterization of the cell membrane of walker-256 carcinosarcoma ascites cells.	cell membranes of carcinosarcoma-256 ascites cells were isolated by the zn++ method. neutral sugars, sialic acid, fucose and n-acetylhexosamines were determined in the cell membrane fraction. cells were agglutinated by wheat germ agglutinin, concanavalin a, and the lectins from phaseolus vulgaris and glycine max. no agglutination was observed with peanut lectin (arachis hypogaea). gel electrophoresis under dissociating conditions revealed more than 40 bands in the membrane fraction after silver ...	1989	2486452
adherence of pseudomonas aeruginosa to tracheal epithelium.	adherence of mucoid and nonmucoid strains of pseudomonas aeruginosa to tracheal epithelium was studied with a perfused-trachea model. the species specificity of adherence was studied by infecting tracheas from hamsters, guinea pigs, or mice. perfused tracheas from hamsters were infected with strains of p. aeruginosa in the presence of various sugars, lectins, cations, or charged polymers. adherence of mucoid strains of p. aeruginosa was greatest for guinea pigs; that for hamsters and mice was ap ...	1989	2494109
regional distribution of glycoconjugates in normal, transitional and neoplastic human colonic mucosa. a histochemical study using lectins.	regional distribution of glycoconjugates in normal and neoplastic colonic mucosa was studied by means of eight lectins: dolichos biflorus (dba), glycine max (sba), triticum vulgare (wga), arachis hypogaea (pna), griffonia simplicifolia-i (gs-i), canavalia ensiformis (con a), limax flavus (lfa), and ulex europaeus-i (uea-i). the lectin binding patterns were examined in 40 normal colonic mucosa (nm) (12 proximal (p) and 28 distal (d], 38 carcinomas (15 p and 23 d), and 31 transitional mucosa (tm) ...	1989	2505446
[some chemical and serological properties of non-dialysable fractions of human seminal plasmas].	some chemical and serological properties of water-soluble and undialysable fraction (wsf) and water-insoluble and undialysable fraction (wisf) of human seminal plasmas were studied. both of the fractions contained proteins as the main components and also carbohydrates as minor components, and each fraction gave 9 bands which were stained with the periodate-schiff and/or coomassie brilliant blue reagents on sodium dodecyl sulfate-polyacrylamide slub gel electrophoresis. wsf reacted with 17 of 19 ...	1989	2515272
o-glycosylation of leukosialin in k562 cells. evidence for initiation and elongation in early golgi compartments.	the o-glycosylation of leukosialin, a major sialoglycoprotein found on leukocytes, has been studied in the human erythroleukemic cell line k562. the appearance of its o-linked chains has been followed in pulse-chase experiments with [35s]methionine by immunoprecipitation with an anti-peptide antiserum as well as with a lectin from salvia sclarea seeds (ssa) specific for galnac-ser/thr and the peanut (arachis hypogaea) agglutinin (pna) which recognizes gal beta 1----3galnac-ser/thr structures. an ...	1989	2526734
comparison of antimalarial efficacy of artemisinin (qinghaosu) and arteether against plasmodium cynomolgi b infection in monkeys.	arteether (30:70 mixture of alpha and beta epimers) used as injectable intramuscular preparation (in neutralized ground-nut oil) had curative action at 5 mg/kg x 3 d against blood-induced plasmodium cynomolgi b infection in rhesus monkeys, while the curative dose of artemisinin (qinghaosu) oil suspension was 10 mg/kg x 7 d or 20 mg/kg x 3 d. parasite clearance with arteether was achieved within 24 h. neither arteether nor artemisinin exhibited any causal prophylactic or radical curative action u ...	1989	2603208
characterization of a neuraminidase from corynebacterium aquaticum responsible for th polyagglutination.	th polyagglutinability is characterized by the agglutination of the red blood cells (rbc) by arachis hypogaea, medicago disciformis, vicia cretica but, in contrast to the t phenomenon, not by glycine max (glycine soja). because th transformation of rbc has been obtained in vitro, the mechanism of th polyagglutinability expression has been studied and reproduced experimentally. an enzyme with neuraminidase specificity has been isolated from the culture supernatant of corynebacterium aquaticum, an ...	1989	2617954
exposure of cryptantigens on red blood cell membranes in patients with acquired immune deficiency syndrome or aids-related complex.	patients with acquired immune deficiency syndrome (aids) or aids-related complex (arc) are subject to recurrent and severe infections due to organisms known to cause red blood cell membrane modifications. these red cell modifications include the exposure of novel carbohydrate cryptantigens that can react with naturally occurring antibodies and potentially result in hemolysis. we examined the frequency of cryptantigen exposure on the surface of red cells from aids/arc patients. blood samples from ...	1989	2656988
lectin-binding sites in neoplastic and non-neoplastic colonic mucosa of 1,2-dimethylhydrazine-treated rats.	qualitative changes of glycoconjugates in luminal surface and goblet cell mucin from colon mucosa of 1,2-dimethylhydrazine (dmh)-treated rats were studied. eight fluoresceinated lectins were used: dolichos biflorus (dba), glycine max (sba), triticum vulgare (wga), limax flavus (lfa), arachis hypogaea (pna), griffonia simplicifolia-i (gs-i), ulex europaeus-i (uea-i) and canavalia ensiformis (con a). the lectin-binding patterns were studied in tumors arising in proximal and distal portions of the ...	1989	2689774
studies on in vitro degradation and in vivo digestion of a slow ammonia releasing urea product.	a slow ammonia releasing urea product (uromalt) has been produced by autoclaving and drying of germinated barley and urea. in vitro degradability of nitrogen (ivdn, raab et al., 1983) was in average 62 +/- 9% in 4 hours, compared to 76 +/- 8% with an autoclaved and dried mixture of urea and not-germinated barley. mixtures of urea with leucaena l. or azadarichta indica (neem cake) showed degradabilities of about 80 and 93%, respectively, when dried at 39 degrees c, but of 65% when dried at 100 de ...	1989	2735818
lectin binding sites in normal rat ovary and enu-induced sertoli cell tumors of the ovaries.	a panel of seven fluorescein isothiocyanate (fitc) labeled lectins were used to study the distribution of specific binding sites in histologic sections of rat ovaries and enu-induced sertoli cell tumors (sct) of the ovaries. ten sct and 5 normal ovaries derived from berlin druckey iv (bd-iv) rats were examined by fitc lectins. the tissues examined were fixed in 10% buffered formalin and embedded in paraffin blocks. in normal ovaries, lectin binding sites were more uniform, ordered and consistent ...	1989	2764514
detection of glycated polypeptides in human aqueous humor by lectin-binding analysis.	the proteins from 30 samples of normal human aqueous humor were separated into major fractions by sodium dodecyl-sulfate polyacrylamide gel electrophoresis, transferred to nitrocellulose, and probed with lectins to characterize the glycated polypeptides. the lectins used were derived from canavalia ensiformis (concanavalin-a), phaseolus vulgaris (erythroagglutinin), arachis hypogaea (peanut agglutinin), and ulex europaeus agglutinin-i. using this microanalytical technique, the glycated polypepti ...	1989	2767173
isolation and characterization of amaranthin, a lectin present in the seeds of amaranthus caudatus, that recognizes the t- (or cryptic t)-antigen.	a lectin (amaranthin) present in the seeds of amaranthus caudatus has been isolated by fractionation on deae-cellulose followed by affinity chromatography on synsorb-t beads (gal beta 1,3galnac alpha-o-r-synsorb). the lectin appeared homogeneous by gel electrophoresis at ph 4.3 and gave a single protein band by sodium dodecyl sulfate-polyacrylamide gel electrophoresis with mr = 33,000-36,000. a native mr = 54,000 was determined by gel filtration suggesting that amaranthin exists as a homodimer. ...	1989	2777780
lectins as probes to pneumocystis carinii surface glycocomplexes.	the binding characteristics of a panel of commercially available fitc-conjugated lectins to pneumocystis carinii (pc) were assessed by fluorescence microscopy and flow cytometry. rat pc obtained from infected lung homogenates were incubated with fitc-conjugated lectins in a series of concentrations, counterstained with propidium iodide, and analyzed for percent fluorescence and fluorescence intensity. all organisms bound concanavalin a and wisteria floribunda agglutinin, 2 representatives of the ...	1989	2785201
early development of the representation of the body surface in si cortex barrel field in neonatal rats as demonstrated with peanut agglutinin binding: evidence for differential development within the rattunculus.	physiological studies have demonstrated a highly organized somatotopic representation of the body surface in si cortex of rat. this representation is correlated morphologically with the presence of barrel-shaped structures in layer iv. conventional staining techniques reveal barrels in the latter part of the first postnatal week. recently, the peroxidase conjugates of lectins, which recognize glycosylated molecules, have been used to study barrel field formation. con a, for example, has been sho ...	1989	2792289
studies of lectin binding to the human cervix uteri: i. normal cervix.	lectins of bauhinia purpurea (bpa), canavalin ensiformis (con a), griffonia simplicifolia i (gs i), griffonia simplicifolia ii (gs ii), maclura pomifera (mpa), arachis hypogaea (pna), glycine max (sba), ulex europaeus i (uea i) and triticum vulgaris (wga) were used to evaluate cell surface carbohydrates in formalin-fixed paraffin-embedded tissue sections of normal human cervix uteri. consistent patterns of staining of the squamous epithelium were obtained in all 30 cases with bpa, gs ii, mpa, pn ...	1989	2793521
specificity of aspartate aminotransferases from leguminous plants for 4-substituted glutamic acids.	aspartate aminotransferase (glutamate-oxalacetate transaminase) was partially purified from extracts of germinating seeds of peanut (arachis hypogaea), honey locust (gleditsia triacanthos), soybean (glycine max), and sophora japonica. the ability of these enzyme preparations, as well as aspartate aminotransferase purified from pig heart cytosol, to use 4-substituted glutamic acids as amino group donors and their corresponding 2-oxo acids as amino group acceptors in the aminotransferase reaction ...	1989	16666674
effects of ddt on the growth of crop plants.	the effects of ddt on the germination and growth of plants were studied using many crop species. of the species tested, oil-rich seeds of plants, such as peanut (arachis hypogaea) and mustard (brassica juncea), were more prone to ddt induced inhibition of germination and subsequent plant growth than cereals, pulses and fibre crops, like rice (oryza sativa), barley (hordeum vulgare), mung bean vigna radiata), pigeon pea (cajanus cajan) and cotton (gossypium hirsutum). studies with (14)c labelled ...	1989	15092369
acute tubulointerstitial nephritis in children: clinical, morphologic, and lectin studies. a report of the southwest pediatric nephrology study group.	clinical histories and renal biopsies were reviewed in 12 children with acute tubulointerstitial nephritis, which was drug related in eight, idiopathic in one, and multifactorial in three. presentation with rashes and hypertension was most common in patients with drug-associated nephritis. eosinophils, which were present in the majority of the renal biopsies, did not distinguish between drug-related and non-drug-related disease. the majority of the children had a good outcome irrespective of the ...	1989	2688404
role of carbohydrate moieties in peanut (arachis hypogaea) peroxidases.	the activities of a cationic (c.prx) and an anionic peroxidase isolated from peanut (arachis hypogaea)-cell suspension culture were drastically reduced when they were deglycosylated with glycopeptidase f or oxidized by 10 mm-periodate. in contrast with the controls, the deglycosylated or the oxidized peroxidases were much more susceptible to proteolytic degradation. in radiolabelling experiments with [35s]methionine, the non-glycosylated c.prx was synthesized in the tunicamycin-treated cultures ...	1989	2604691
th activation of maternal and cord blood.	th activation of red cells is characterized by agglutination with the peanut lectin from arachis hypogaea and is diminished by treatment with proteolytic enzymes. the first cases of th activation were associated with bacterial infections. more recently, a high incidence of th activation in congenital hypoplastic anemia has been reported, along with the finding that 13.5 percent of cord bloods are th activated. the incidence of th reactivity in newborn infants was confirmed by studying 200 paired ...	1989	2773030
[lectin-binding properties of the neuroepithelium of the vomeronasal organ, olfactory epithelium proper and the septal organ of masera in mice (semithin section study)].	the apical border of the vomeronasal neuroepithelium, the olfactory epithelium proper, and the septal organ possess varying lectin-binding properties. this can be judged by their ability to bind a peculiar lectin and/or by their reactivity to the given lectin. the following lectins have been used: triticum vulgaris agglutinin (wga), ulex europeus agglutinin (uea-1), arachis hypogea agglutinin (pna), lymbus polyphenus agglutinin (lpa), glycine soja agglutinin (sba) and dolchos diflerus agglutinin ...	1989	2604560
thermal regulation of phosphoenolpyruvate carboxylase and ribulose-1,5-bisphosphate carboxylase in c(3) and c(4) plants native to hot and temperate climates.	exposure of leaf sections from 2-week-old seedlings of sorghum (sorghum bicolor l.) (c(4) plant), corn (zea mays l.) (c(4)), peanut (arachis hypogaea l.) (c(3) plant), and soybean (glycine max l.) (c(3)) to 40 or 45 degrees c for up to 4 hours resulted in significant increases in the levels of 102 kilodalton (c(4)), 52 kilodalton (c(3) and c(4)), and 15 kilodalton (c(3) and c(4)) polypeptides. these proteins comigrated, respectively, with authentic phosphoenolpyruvate carboxylase (pepc) and the ...	1989	16666926
evidence of altered structural and secretory glycoconjugates in the jejunal mucosa of patients with gluten sensitive enteropathy and subtotal villous atrophy.	the pattern of lectin histochemistry in formalin fixed, paraffin embedded normal jejunal and subtotal villous atrophy specimens from patients with gluten sensitive enteropathy were compared. there was no significant difference in the binding pattern of five lectins (arachis hypogaea, canavalia ensiformis, lens culinaris, phaseolus vulgaris and triticum vulgaris) between normal and abnormal specimens. there were significant changes in the binding pattern of three lectins (dolichos biflorus, ulex ...	1989	2753405
mevalonate-metabolizing enzymes in arachis hypogaea.	the activities of the mevalonate metabolizing enzymes-hmg-coa reductase, mevalonate kinase, mevalonate phosphokinase and mevalonate pyrophosphate decarboxylase -were assayed with the respective substrates in green seedlings of arachis hypogaea. mvapp decarboxylase is the rate-limiting step among these enzymes and is inhibited by phenolic acids. its activity in the seedlings was found to decrease in the absence of light and on treatment with abscisic acid. these results suggest that regulation of ...	1989	2528059
plant regeneration from immature embryos of peanut.	plant regeneration from immature embryos of peanut (arachis hypogaea l.) can be accomplished through somatic embryogenesis. the highest frequency of somatic embryo formation occurred on b5 medium plus 0.5-1.0 mg/l picloram. shoots and plants developed from the somatic embryos only after extended culture on basal medium. shoots were excised from thick embryonic roots and rerooted on murashige and skoog medium containing half the normal concentration of inorganic salts. this technique should be us ...	1989	24233140
effects of crop rotation and nonfumigant nematicides on peanut and corn yields in fields infested with criconemella species.	the effects of nematicide treatments and corn-peanut cropping sequences on the population development of criconemella ornata, and c. sphaerocephala and the related impact on crop yields were investigated at two north carolina locations. criconemella ornata and c. sphaerocephala were present at the norman perry farm, bertie county (bertie); however, only c. ornata was found at the central crops research station, johnston county (ccrs). an untreated control was compared to aldicarb 15g, carbofuran ...	1989	19287608
vanadate mimics effects of fungal cell wall in eliciting gene activation in plant cell cultures.	cell-suspension cultures of peanut (arachis hypogaea l.) can be used as a very sensitive and rapidly responding physiological system for monitoring extracellular signals. elicitors effect the activation of the genes that code for a set of enzymes synthesizing stilbenes. within 2-6 h after administering micromolar, concentrations of orthovanadate to the suspended cells, the enzyme activities of phenylalanine ammonia-lyase, stilbene synthase, and cinnamate 4-hydroxylase increased 10-to 100-fold. t ...	1989	24212338
lectin histochemistry of ameloblastomas and odontogenic keratocysts.	the cell membrane carbohydrate components of 10 simple (follicular and/or plexiform pattern) and 5 acanthomatous ameloblastomas, one plexiform unicystic ameloblastoma, one soft tissue ameloblastoma and 11 odontogenic keratocysts were studied in paraffin-embedded tissues using horseradish peroxidase-conjugated lectins. the presence of glucose and mannose was demonstrated by intense labelling with concanavalin ensiforme (con a) in 73% of the ameloblastomas examined, while periodate oxidation of th ...	1989	2473205
distribution of lectin binding glycoprotein in osteoclasts.	arachis hypogaea (pna) lectin, specific for gal-b-1,3-galnac disaccharide and wheat germ (wga) lectin, specific for (glcnac) and terminal neuraminic acid were used to identify histiocytic giant cells, osteoclast like giant cells and osteoclasts. pna lectin, without neuraminidase predigestion was not bound by the giant cells, while they showed a strong reaction with wga lectin. neuraminidase pretreatment decreased wga lectin binding, which supports that neuraminic acid plays a role in the binding ...	1989	2925448
renal cystic disease of infancy: results of histochemical studies. a report of the southwest pediatric nephrology study group.	histochemical techniques utilizing tetragonolobus lotus (proximal tubules), arachis hypogaea (distal nephron, i.e., distal convoluted tubules and collecting ducts), and antibodies against tamm-horsfall protein (thick ascending limbs of henle) were used to determine the site of origin of renal cysts in five children with autosomal recessive polycystic kidney disease (arpkd) and three patients with glomerulocystic disease (gcd) presenting in the 1st year of life. the findings support a distal neph ...	1989	2702085
paralongidorus bullatus n. sp. from groundnut soils in niger and comments on xiphinema parasetariae luc.	paralongidorus bullatus n. sp. from groundnut soils in sador'é niger is described and illustrated. it is distinguishable from most species of the genus by its body and odontostyle lengths, knob-like head, and digitate ending of the protoplasmic region of the tail. length of the female body is 4.4-5.5 mm, of the odontostyle 132-156 mum, and of the tail 32-44 mum. tail terminus is conoid to broadly rounded. uteri are well developed, without sperms. males are not found. xiphinema parasetariae luc 1 ...	1990	19287761
purification of isozymes of superoxide dismutase from groundnut (arachis hypogea) seedlings.	groundnut seedlings contain five isozymes of superoxide dismutase. these isozymes were purified to homogeneity by ammonium sulfate precipitation, ionexchange chromatography on diethyl amino ethyl cellulose, gel filtration using sephadex g100 and preparative gel electrophoresis. manganese containing superoxide dismutase showed optimal activity at ph 7.8 whereas activity was one fifth at ph 9.8. this difference in the activity was not observed in case of copper-zinc enzymes.	1990	2282075
influence of lectins on female sex pheromone reception byheterodera schachtii (nematoda: heteroderidae) males.	a bioassay was developed in which the chemotactic behavior of males of the sugarbeet cyst nematodeheterodera schachtii towards the female sex pheromone can be observed and documented at any time. the standardized test, performed under sterile conditions, gives reproducible results within 1-2 hr. incubation of males with the lectins fromcanavalia ensiformis, triticum vulgare, arachis hypogaea, helix pomatia, andlimax flavus did not affect chemotactic attraction to the sex pheromone. supplementing ...	1990	24264204
nonparametric approach to multitrait selection for yield in groundnut (arachis hypogaea l.).	eight characters related to nitrogen fixation and pod development measured 30 days after flowering were evaluated for their correct grading of the relative yield performance of 17 genetically diverse lines of groundnut (arachis hypogaea l.). each line was assigned a high or low yield status based on its pod yield, shelling percentage, and 100-kernel weight. seventeen character combinations were examined for their relative merit in correct identification of the yield status of lines. the characte ...	1990	24220899
[constituents of the seed coat of arachis hypogaea l].	from the seed coat of arachis hypogaea two compounds were isolated. they were identified as the dimer of proanthocyanidin-a type(i) and d-(+)-catechin (ii) on the basis of uv, ir, ms, cd, 1h-nmr, 13c-nmr and chemical evidences. it has been found that ii is the effective constituent of this plant and i has some active effects as well.	1990	2206388
an investigation of the dissociation and denaturation of legumin by salts using laser light scattering and circular dichroism spectroscopy.	the dissociation of legumin, a 12 s seed storage globulin from pisum sativum, has been studied by laser light scattering and circular dichroism spectroscopy. salts from the hofmeister series, in particular sodium perchlorate, were used as dissociating agents. the mr 360,000 hexameric protein was found to dissociate first to trimers and further to monomers and the number of amino acids involved in the trimer-trimer interaction estimated to be 23(+/-4). native legumin appears to be more strongly b ...	1990	2153027
isolation and characterization of blood group n antigen precursor glycoproteins with thomsen-friedenreich (t) activity, n antigen precursor glycoproteins and t-active glycoproteins from cyst fluids of malignant ovarian clear cell carcinoma.	1. two perchloric acid-soluble glycoprotein fractions from cyst fluids of 2 patients with ovarian clear cell carcinoma in malignant were subjected to a systematic affinity chromatography using vicia unijuga lectin-cellulofine column and arachis hypogaea lectin-cellulofine column and separated into four glycoproteins, blood group n antigen precursor glycoprotein with thomsen-friedenreich (t) activity, n antigen precursor glycoprotein, t-active glycoprotein and serologically inactive glycoprotein, ...	1990	2282960
separation and biochemical characterization of blood group n antigen precursor glycoproteins with thomsen-friedenreich (t) activity, t-active glycoproteins and n antigen precursor glycoproteins from ascites of primary ovarian cancer patients.	1. three perchloric acid-soluble fractions from ascites of three primary ovarian cancer patients were subjected to sephacryl s-300 gel filtration, respectively, and three fr. 1 which were eluted in the vicinity of void volume as minor fractions, were then separated by a systematic affinity chromatography using vicia unijuga lectin-sepharose cl-4b column and arachis hypogaea lectin-sepharose cl-4b column into three glycoproteins, blood group n antigen precursor glycoprotein with thomsen-friedenre ...	1990	2257955
localization of plant lipids for light microscopy using p-phenylenediamine in tissues of arachis hypogaea l.	p-phenylenediamine (ppd) can be used en bloc to preserve and differentiate cell lipids in aldehyde-fixed peanut plant tissues treated with osmium tetroxide during dehydration in 70% ethanol. semithin plastic sections for light microscopy need no further staining and can be mounted in histoclad after drying on a slide. brown staining above background differentiates lipid-containing structures. nonspecific staining can be distinguished in control preparations extracted en bloc with lipid solvents.	1990	1694315
expression of a stilbene synthase gene in nicotiana tabacum results in synthesis of the phytoalexin resveratrol.	a gene from groundnut (arachis hypogaea) coding for stilbene synthase was transferred together with a chimaeric kanamycin resistance gene. it was found to be rapidly expressed after induction with uv light and elicitor in tobacco cells (nicotiana tabacum). comparative studies of stilbene synthase mrna synthesis in groundnut and transgenic tobacco suspension cultures revealed the same kinetics of gene expression. stilbene synthase specific mrna was detectable 30 minutes after elicitor induction a ...	1990	2103451
effect of gamma-interferon on lectin-binding glycoproteins in cultured human keratinocytes.	we report the effect of exposure of human keratinocyte cultures to human recombinant gamma-interferon (g-ifn) on the expression of glycoproteins. concanavalia ensiformis agglutinin (con-a), and arachis hypogaea agglutinin (pna) were used to investigate expression of glycoproteins. np-40 extracts from cultures grown with or without 100 u/ml g-ifn were analyzed by incubation of sds-polyacrylamide gels with 125i-labeled lectins. comparison of con-a binding glycoprotein profiles showed both qualitat ...	1990	2115319
lectins and heparin-binding features of human spermatozoa as analyzed by flow cytometry.	sperm cell surface characteristics may serve as a useful diagnostic feature for the evaluation of seminal samples, but to date no useful analytical method(s) is available for their routine quantitative analysis. fluorescein isothiocyanate-conjugated lectins, proteins with unique and specific saccharide-binding characteristics, and heparin are potentially useful markers for such an analytical procedure. a protocol is outlined whereby frozen semen is thawed and treated with either fluorescein isot ...	1990	2124086
[a study of the t-antigen in ovarian tumor].	by means of the avidin-biotin-peroxidase complex (abc) method with peanut lectin (pna, arachis hypogaea), the role of t-antigen in the human ovarian tumor was investigated and the following results were obtained. 1) among benign tumors, 76.9% cases were t-antigen negative and cryptic t-antigen positive. 2) among malignant ovarian tumors, 55.2% cases were t-antigen positive and 20.7% cases were negative for both t-antigen and cryptic t-antigen. 3) in malignant ovarian tumors, the lower the tissue ...	1990	2153744
autosomal recessive polycystic kidney disease: characterization of human peritoneal and cystic kidney cells in vitro.	renal cystic epithelia and peritoneal mesothelia from two humans with autosomal recessive polycystic kidney disease (arpkd) were grown in culture. cystic epithelial and mesothelial cells formed continuous monolayers in vitro. by electron microscopy, cystic renal cells exhibited a single apical cilium and numerous short, stubby microvilli, both in situ and in vitro. mesothelial cells exhibited intra- and extracellular membrane-limited, lipid-filled vesicles and surface microvilli. cystic kidney c ...	1990	2154094
characterization of an in vitro system of human renal papillary collecting duct cells.	we have developed an in vitro model of human papillary collecting duct cells isolated from cadaver kidneys using methods similar to those we previously reported for the isolation of human proximal tubule cells. to date we have isolated papillary collecting duct cells from 100 normal human kidneys. papillae were dissected and digested in cellgro containing 400 u/ml collagenase. cells were plated on fibronectin-coated culture flasks at a density of 10(4) live cells/ml in cellgro supplemented with ...	1990	2161826
[separation of thomsen-friedenreich (t) antigen and n antigen precursor glycoproteins from perchloric acid-soluble fraction of cyst fluid of human ovarian clear cell carcinoma and their some chemical and serological properties].	one m perchloric acid-soluble fraction separated from cyst fluid of a patient (blood group ab) with ovarian clear cell carcinoma in malignant was subjected to sephacryl s-200 gel filtration and fraction 1 which was eluted at void volume, reacted with both of the lectins of vicia unijuga and arachis hypogaea. this fraction was then separated into 6 fractions (deae-frs. 1-6) in a deae-sephacel column chromatography. deae-frs. 3-6 were sugar-rich glycoprotein fractions which contained galactose and ...	1990	2165527
biochemical characterization of glycoprotein components in human ovarian cyst fluids by lectins.	1. perchloric acid-soluble glycoprotein fractions (pasfs) were separated, in yields of 180-610 mg per 100 ml of cyst fluid, from the cyst fluids of human ovarian cystadenomas (ocas) in benign and borderline and ovarian clear cell carcinoma (occ) in malignant, and then identified as glycoproteins with 19.1-69.5% carbohydrate by their chemical composition analyses. 2. these pasfs reacted with anti-a, -b, -lea and/or -leb sera, but did not react with anti-h, -m and -n sera. all of the pasfs reacted ...	1990	2165928
application of flow cytometry to studies on the human acrosome.	this study describes the use of flow cytometry combined with specific labelling of the human sperm acrosome using a fitc-labelled plant lectin (arachis hypogea agglutinin). localization of the label to the acrosome was encouraged by freezing the sperm for at least 24 hours at -70 degrees c prior to labelling. studies of sperm from 53 normospermic men revealed that acrosome labelling followed a single normal distribution without the presence of subpopulations. the average fluorescence and degree ...	1990	2211342
lectin typing of campylobacter isolates.	isolates of campylobacter jejuni, c coli, c fetus and c laridis were tested for agglutination reactions with a panel of five lectins: arachis hypogaea, bauhinia purpurea, solanum tuberosum, triticum vulgaris and wisteria floribunda. twenty three patterns of agglutination (lectin types) were recorded among 376 isolates. patterns were consistent and reproducible. only 4.5% of isolates were untypable because of autoagglutination. some lectin types were found exclusively or predominantly in a specie ...	1990	2262570
stimulation of vascular cell proliferation by beta-galactoside specific lectins.	an investigation was conducted to assess the effects of various beta-galactoside specific lectins on the growth of vascular cells in vitro. the plant lectins from peanut (arachis hypogaea), mushroom (agaricus bisporus), and coral tree (erythrina corallodendron) were used in these studies with the ultimate purpose of comparing those findings with data derived with the lectin isolated from rat lung. peanut lectin was added to confluent and subconfluent cultures of smooth muscle cells (smc), pulmon ...	1990	2379767
differences in expression of oligosaccharide determinants by phenotypically distinct sublines of the dunning 3327 rat prostate cancer.	oligosaccharides expressed by the 3327-h and 3327-mat lylu sublines of the dunning rat prostate cancer model have been compared in formalin-fixed and routinely paraffin-embedded tumour tissues. binding by lectins of defined specificity has been employed to identify expression of seven oligosaccharide structures by primary and metastatic prostatic carcinoma cells. neuraminidase digestion was employed to reveal determinants masked by sialic acid. the presence of core man alpha 1----3(man alpha 1-- ...	1990	2388349
effect of peanut tannin extracts on growth of aspergillus parasiticus and aflatoxin production.	twenty-three peanut (arachis hypogaea l.) genotypes were evaluated for kernel resistance to aspergillus parasiticus spear. colonization and aflatoxin contamination when incubated under high relative humidity. also, tannin-containing extracts from kernel coats (testae) and cotyledons of these genotypes were prepared and tested for their effect on a. parasiticus growth and aflatoxin production in vitro. the lowest degree of colonization, less than 30%, was noted in kernels from the genotypes, toal ...	1990	2388679
glycoconjugate histochemistry in the glycocalyx of guinea pig middle ear mucosa.	an attempt was made to determine the composition of oligosaccharide side chains in the apical cell surface glycocalices of the middle ear epithelium. this glycoconjugate is located on the innermost luminal surface, but its function is still unknown. the lectins evaluated included triticum vulgare, canavalia ensiformis, maclura pomifera, arachis hypogaea, ricinus communis i (rca-i), and limax flavus. the staining results obtained with the six different lectin probes in the lowicryl k4m-embedded g ...	1990	2393562
changes in glycoconjugates revealed by lectin staining in the developing airways of syrian golden hamsters.	lectin binding was studied in the developing airways of syrian golden hamsters on gestational days 11-16 (day 16 is the day of birth). the trachea and lungs were fixed in 4% formaldehyde-1% glutaraldehyde, 6% mercuric chloride-1% sodium acetate-0.1% glutaraldehyde, and 95% ethanol; embedded in paraffin; and stained with eight lectin-horseradish peroxidase conjugates: triticum vulgare (wga), dolichos biflorus (dba), helix pomatia (hpa), maclura pomifera (mpa), griffonia simplicifolia i-b4 (gsa i- ...	1990	1700650
lectin histochemical characteristics of the canine female mammary gland.	twelve biotinylated lectins and an avidin-biotin-peroxidase method were used to detect and localize specific carbohydrate residues on formalin-fixed, paraffin-embedded female canine mammary gland sections. histologic sections from 3 lactating and 7 nonlactating mixed-breed dogs (age 5.6 +/- 0.35 years) were incubated with arachis hypogea agglutinin (peanut agglutinin; pna), concanavalia ensiformis agglutinin (cona), dolichos biflorus agglutinin (dba), glycine max agglutinin (sba), griffonia simp ...	1990	1700652
elicitor-specific induction of one member of the chitinase gene family in arachis hypogaea.	chitinases are believed to play an important role in plant defence against bacterial and fungal attack. in peanut (arachis hypogaea) chitinase genes form a small multigene family. four chitinase cdnas (chit 1-4) were isolated from cultured peanut cells. expression of individual chit genes was assayed by the polymerase chain reaction (pcr) followed by analysis of restriction fragment length polymorphisms (rflp). uv irradiation, dilution of cell cultures and treatment with phytophthora megasperma ...	1990	1980004
in vitro embryo explant cultures of peanut to evaluate resistance to ditylenchus destructor.	population densities of d. destructor on embryo explants of 22 peanut genotypes grown in vitro were compared with those in roots and seeds of the same genotypes grown in the greenhouse. during the first 8 weeks after inoculation, the optimum incubation period was 6 weeks for maximum reproduction of ditylenchus destructor on embryo explants of peanut (arachis hypogaea l. cv. sellie) inoculated with 250 nematodes at 25 c. nematode numbers increased 17-fold. deletion of mnso ho and a higher khpo co ...	1990	19287727
host status of seven weed species and their effects on ditylenchus destructor infestation of peanut.	the host suitability to ditylenchus destructor of seven common weed species in peanut (arachis hypogaea) fields in south africa was determined. based on the number of nematodes per root unit, white goosefoot (chenopodium album), feathertop chloris (chloris virgata), purple nutsedge (cyperus rotundus), jimson weed (datura stramonium), goose grass (eleusine indica), khaki weed (tagetes minuta), and cocklebur (xanthium strumarium) were poor hosts. ditylenchus destructor survived on all weed species ...	1990	19287723
histopathology of ditylenchus destructor on peanut.	the time and mode of entry, and development of ditylenchus destructor in peanut were studied in field and greenhouse experiments. few nematodes were present in the cortex of the roots. at 90-120 days after planting, d. destructor was observed in the exocarp at the base of the pod near the point of connection with the peg. the peg was invaded from this primary infection site. the endocarp of the hull was usually penetrated through openings at the base of the mesocarp and sometimes at the pod apex ...	1990	19287720
morphological comparison of three host races of meloidogyne javanica.	a morphological and morphometric comparison using light microscopy and scanning electron microscopy was made of six populations of meloidogyne javanica belonging to three host races (infective on pepper, peanut, or noninfective on both). the variability of certain morphological characters was studied within these populations, and the reliability of these taxonomic traits was evaluated for usefulness in species identification. the most useful diagnostic characters of m. javanica were head and sty ...	1990	19287689