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composting of waste paint sludge containing melamine resin and the compost's effect on vegetable growth and soil water quality.melamine resin (mr) is introduced to the environment from many industrial effluents, including waste paint sludge (wps) from the automobile industry. melamine resin contains a high nitrogen (n) content and is a potential n source during composting. in this study, two carbon sources, waste paper (wp) and plant residue (pr), were used to study their effects on composting of wps. additional work tested the wps-composts effects on plant growth and soil water quality. after 84 days of composting, 85% ...201223127275
interaction of brassinosteroids and polyamines enhances copper stress tolerance in raphanus sativus.brassinosteroids (brs) and polyamines (pas) regulate various responses to abiotic stress, but their involvement in the regulation of copper (cu) homeostasis in plants exposed to toxic levels of cu is poorly understood. this study provides an analysis of the effects of exogenously applied brs and pas on radish (raphanus sativus) plants exposed to toxic concentrations of cu. the interaction of 24-epibrassinolide (ebr, an active br) and spermidine (spd, an active pa) on gene expression and the phys ...201222915739
contractile effect of radish and betel nut extracts on rabbit gallbladder.raphanus sativus (abbreviated in this paper as rs.cr) and areca catechu (ac.cr), commonly known as radish and betel nut respectively, are traditionally used in south asia for different gastrointestinal, gallbladder, and hepatic diseases. there has not been any study to see how they modulate gallbladder contractility. we selected isolated rabbit gallbladder tissue preparations, mounted them in tissue baths containing krebs-henseleit solution at 37°c, and then recorded the changes in baseline tone ...201222499720
insecticide resistance mechanisms in the green peach aphid myzus persicae (hemiptera: aphididae) ii: costs and benefits.among herbivorous insects that have exploited agro-ecosystems, the peach-potato aphid, myzus persicae, is recognized as one of the most important agricultural pests worldwide. uses over 400 plant species and has evolved different insecticides resistance mechanisms. as m. persicae feeds upon a huge diversity of hosts, it has been exposed to a wide variety of plant allelochemicals, which probably have promoted a wide range of detoxification systems.201222685539
phytotoxicity of new furan-derived aminophosphonic acids, n-aryl furaldimines and 5-nitrofuraldimine.the aim of this work was to synthesize selected furaldimines and their aminophosphonic derivatives and evaluation the phytotoxicity of new obtained products according to oecd 208 guideline. four schiff bases, n-furfurylidene-p-anisidine (1a), n-furfurylidene-p-toluidine (1b), n-furfurylidene-benzhydrylamine (1c), and n-(2-nitrofurfurylidene)-p-toluidine (1d) were synthesized and three new furan-derived n-substituted aminomethylphosphonic acids, namely: 2-furyl n-(p-methoxyphenyl)-aminomethylphos ...201323869973
comparison of bioactive phytochemical content and release of isothiocyanates in selected brassica sprouts.the consumption of brassica sprouts as raw vegetables provides a fair amount of glucosinolates (gls) and active plant myrosinase, which enables the breakdown of gls into health-promoting isothiocyanates (itcs). this study reports the determination of the main constituents related to human health found in edible sprouts of two brassica oleracea varieties, broccoli and tuscan black kale, and two raphanus sativus varieties, daikon and sango. radish sprouts exhibited the highest ability to produce i ...201323768361
deciphering the diploid ancestral genome of the mesohexaploid brassica rapa.the genus brassica includes several important agricultural and horticultural crops. their current genome structures were shaped by whole-genome triplication followed by extensive diploidization. the availability of several crucifer genome sequences, especially that of chinese cabbage (brassica rapa), enables study of the evolution of the mesohexaploid brassica genomes from their diploid progenitors. we reconstructed three ancestral subgenomes of b. rapa (n = 10) by comparing its whole-genome seq ...201323653472
effect of flue gas desulfurization residue on plant establishment and soil and leachate quality.effects on soil quality and crop establishment after incorporation of flue gas desulfurization by-product (fgd) into soil as an amendment was assessed in a mesocosm study. mesocosm units received applications equivalent to 0, 2.5, 5.0, 7.5, and 10% fgd residue [0, 25, 50, 75, and 100 tons acre(-1)]. germination, biomass production, and elemental composition of corn (zea mays l. var. dekalb dk-683), soybean [glycine max (l.) merr. var. haskell pupa 94], radish (raphanus sativus l. var. sparkler), ...201311401255
development of a chloroplast dna marker for monitoring of transgene introgression in brassica napus l.chloroplast molecular markers can provide useful information for high-resolution analysis of inter- and intra-specific variation in brassicaceae and for differentiation between its species. combining data generated from nuclear and chloroplast markers enables the study of seed and pollen movement, and assists in the assessment of gene-flow from genetically modified (gm) plants through hybridization studies. to develop chloroplast dna markers for monitoring of transgene introgression in brassica ...201323690044
a real-scale soil phytoremediation.in the present investigation, a phytoremediation process with a combination of different plant species (populus nigra (var.italica), paulownia tomentosa and cytisus scoparius), and natural growing vegetation has been proposed at real-scale (10.000 m(2)) to bioremediate and functionally recover a soil historically contaminated by heavy metals and hydrocarbons. in the attempts to assess both effectiveness and evolution of the remediation system towards a natural soil ecosystem, besides the polluti ...201323179352
comprehensive analysis of expressed sequence tags from cultivated and wild radish (raphanus spp.).radish (raphanus sativus l., 2n = 2× = 18) is an economically important vegetable crop worldwide. a large collection of radish expressed sequence tags (ests) has been generated but remains largely uncharacterized.201324144082
yield, chemical composition and nutritional quality responses of carrot, radish and turnip to elevated atmospheric carbon dioxide.future concentration of carbon dioxide in the atmosphere is very important due to its apparent economic and environmental impact in terms of climate change. however, a compressive assessment of its effect on the nutritional and chemical characteristics of food crops has yet to be established. in the present study the impact of elevated atmospheric co2 on the yield, chemical composition and nutritional quality of three root vegetables, carrot (daucus carota l. cv. t-1-111), radish (raphanus sativ ...201323576218
nucleotide sequence variation of glabra1 contributing to phenotypic variation of leaf hairiness in brassicaceae vegetables.glabra1 (gl1) belongs to the group of r2r3-myb transcription factors and is known to be essential for trichome initiation in arabidopsis. in our previous study, we identified a gl1 ortholog in brassica rapa as a candidate for the gene controlling leaf hairiness by qtl analysis and suggested that a 5-bp deletion (b-allele) and a 2-bp deletion (d-allele) in the exon 3 of brgl1 and a non-synonymous snp (c-allele) in the second nucleotide of exon 3 possibly cause leaf hairlessness. in this study, we ...201323370467
genetic analysis of hybrid seed formation ability of brassica rapa in intergeneric crossings with raphanus sativus.a hybridization barrier leads to the inability of seed formation after intergeneric crossings between brassica rapa and raphanus sativus. most b. rapa lines cannot set intergeneric hybrid seeds because of embryo breakdown, but a b. rapa line obtained from turnip cultivar 'shogoin-kabu' is able to produce a large number of hybrid seeds as a maternal parent by crossings with r. sativus. in 'shogoin-kabu' crossed with r. sativus, developments of embryos and endosperms were slower than those in intr ...201323203221
a new cryptic virus belonging to the family partitiviridae was found in watermelon co-infected with melon necrotic spot virus.a novel virus was detected in watermelon plants (citrullus lanatus thunb.) infected with melon necrotic spot virus (mnsv) using solid next-generation sequence analysis. in addition to the expected msnv genome, two double-stranded rna (dsrna) segments of 1,312 and 1,118 bp were also identified and sequenced from the purified virus preparations. these two dsrna segments encode two putative partitivirus-related proteins, an rna-dependent rna polymerase (rdrp) and a capsid protein, which were sequen ...201323775759
molecular cloning and functional expression of a water-soluble chlorophyll-binding protein from japanese wild radish.hydrophilic chlorophyll (chl)-binding proteins have been isolated from various brassicaceae plants and are categorized into class ii water-soluble chl-binding proteins (wscps). although the molecular properties of class ii wscps including brassica-type (e.g., cauliflower wscp, brussels sprouts wscp and bnd22, a drought- and salinity-stress-induced 22 kda protein of rapeseed), a lepidium-type, and an arabidopsis-type wscps have been well characterized, those of raphanus-type wscps are poorly unde ...201323266282
tandem combination of trigonella foenum-graecum defensin (tfgd2) and raphanus sativus antifungal protein (rsafp2) generates a more potent antifungal protein.plant defensins are small (45 to 54 amino acids) positively charged antimicrobial peptides produced by the plant species, which can inhibit the growth of a broad range of fungi at micro-molar concentrations. these basic peptides share a common characteristic three-dimensional folding pattern with one α-helix and three β-sheets that are stabilized by eight disulfide-linked cysteine residues. instead of using two single-gene constructs, it is beneficial when two effective genes are made into a sin ...201324022215
qtl analysis using snp markers developed by next-generation sequencing for identification of candidate genes controlling 4-methylthio-3-butenyl glucosinolate contents in roots of radish, raphanus sativus l.snp markers for qtl analysis of 4-mtb-gsl contents in radish roots were developed by determining nucleotide sequences of bulked pcr products using a next-generation sequencer. dna fragments were amplified from two radish lines by multiplex pcr with six primer pairs, and those amplified by 2,880 primer pairs were mixed and sequenced. by assembling sequence data, 1,953 snps in 750 dna fragments, 437 of which have been previously mapped in a linkage map, were identified. a linkage map of nine linka ...201323308250
effects of selenium accumulation on phytotoxicity, herbivory, and pollination ecology in radish (raphanus sativus l.).selenium (se) has contaminated areas in the western usa where pollination is critical to the functioning of both agricultural and natural ecosystems, yet we know little about how se can impact pollinators. in a two-year semi-field study, the weedy plant raphanus sativus (radish) was exposed to three selenate treatments and two pollination treatments to evaluate the effects on pollinator-plant interactions. honey bee (apis mellifera l.) pollinators were observed to readily forage on r. sativus fo ...201323000967
special plant species determines diet breadth of phytophagous insects: a study on host plant expansion of the host-specialized aphis gossypii glover.host specialization is a ubiquitous character of phytophagous insects. the polyphagous population is usually composed of some subpopulations that can use only a few closely related plants. cotton-melon aphids, aphis gossypii glover exhibited strong host specialization, and the cotton- and cucurbits-specialized biotypes had been clearly identified. however, the experimental work that addressed the roles of plant species in determining diet breadth of phytophagous insects is rare. in the present s ...201323580128
phytotoxic activity of foliar volatiles and essential oils of calamintha nepeta (l.) savi.foliar volatiles and essential oils of calamintha nepeta (l.) savi, a mediterranean plant species belonging to the labiatae family, were investigated for their phytotoxic activities on seed germination and root growth of crops (lactuca sativa l. and raphanus sativus l.) and weed species (lolium perenne l. and amaranthus retroflexus l.). foliar volatiles of c. nepeta (l.) savi strongly inhibited both germination and root growth of lettuce, and its essential oils, especially at 125, 250 and 500 μl ...201323216166
phytotoxicity of secondary metabolites isolated from flourensia oolepis s.f.blake.the aim of this study was to isolate the active principles of flourensia oolepis s.f.blake (asteraceae), which completely inhibited the germination of raphanus sativus seeds at 10 mg/ml. flavanone pinocembrin and sesquiterpene ilicol, were isolated by bioassay-guided fractionation. they were active both against monocot and dicot seeds. pinocembrin was the most active compound, with an ic50 (germination) value of 0.24, 3.40, 3.28, and 3.55 mm against panicum miliaceum, avena sativa, lactuca sativ ...201323847074
potential allelopathic indole diketopiperazines produced by the plant endophytic aspergillus fumigatus using the one strain-many compounds method.on the basis of the osmac (one strain-many compounds) strategy, 14 indole diketopiperazine (dkp) alkaloids, including spirotryprostatins (1-3), tryprostatins (4-6), and cyclotryprostatins (7-14), were isolated from the endophyte aspergillus fumigatus associated with melia azedarach l. their structures were identified by nuclear magnetic resonance and electrospray ionization mass spectrometry data. all the indole dkps were evaluated for plant growth regulation using the lettuce (lactuca sativa) s ...201324188331
comparative mapping of raphanus sativus genome using brassica markers and quantitative trait loci analysis for the fusarium wilt resistance trait.fusarium wilt (fw), caused by the soil-borne fungal pathogen fusarium oxysporum is a serious disease in cruciferous plants, including the radish (raphanus sativus). to identify quantitative trait loci (qtl) or gene(s) conferring resistance to fw, we constructed a genetic map of r. sativus using an f2 mapping population derived by crossing the inbred lines '835' (susceptible) and 'b2' (resistant). a total of 220 markers distributed in 9 linkage groups (lgs) were mapped in the raphanus genome, cov ...201323864230
carbonaceous soil amendments to biofortify crop plants with zinc.carbonaceous soil amendments, comprising mixtures of biosolids and biochar, have been demonstrated to improve fertility while reducing nitrate leaching. we aimed to determine the efficacy of a biosolids/biochar soil amendment in biofortification of vegetables with zn, an element that is deficient in one third of humanity. we grew beetroot (beta vulgaris), spinach (spinacia oleracea), radish (raphanus sativus), broccoli (brassica oleracea), carrot (daucus carota), leek (allium ampeloprsum), onion ...201323146312
chemical composition and antimicrobial and allelopathic activity of tunisian conyza sumatrensis (retz.) e.walker essential oils.conyza sumatrensis (retz.) e.walker (asteraceae) is a spontaneous annual herb, fairly widespread throughout tunisia, which has rarely been studied or valued in any sector. essential oils were obtained by hydrodistillation of different parts (flower heads, leaves, stems, and roots) of c. sumatrensis plants, which were collected in autumn (november 2007) at the flowering stage in the area of monastir, tunisia. in total, 98 compounds, representing 88.1-99.3% of the oil composition, were identified ...201323418168
development of a pcr-based method for monitoring the status of alcaligenes species in the agricultural environment.to analyze the status of the genus alcaligenes in the agricultural environment, we developed a pcr method for detection of these species from vegetables and farming soil. the selected pcr primers amplified a 107-bp fragment of the 16s rrna gene in a specific pcr assay with a detection limit of 1.06 pg of pure culture dna, corresponding to dna extracted from approximately 23 cells of alcaligenes faecalis. meanwhile, pcr primers generated a detectable amount of the amplicon from 2.2×10(2) cfu/ml c ...201424670615
biotechnological production of inducible defense-related proteins in edible radish (raphanus sativus) found in nepal.fungal infection in plant leads to use of many hazardous antifungal chemicals. alternative to these chemicals, defense related antifungal proteins can be used in case of fungal diseases.201424596739
antifungal, insecticidal, and plant growth promoting potential of streptomyces hydrogenans dh16.in the present study, an actinobacterium strain, possessing antagonistic activity against different fungal phytopathogens viz. colletotrichum acutatum, cladosporium herbarum, alternaria brassicicola, exserohilum sp., alternaria mali, colletotrichum gleospoiroides, alternaria alternata, cercospora sp., fusarium oxysporum f.sp. dianthi and fusarium moniliformae, was isolated from soil and identified as streptomyces hydrogenans dh16. application of culture supernatant (5%)/cells (10(7)  cfu ml(-1) ...201423765423
microtubules in root hairs.the microtubules of root hairs of raphanus sativus, lepidium sativum, equisetum hyemale, limnobium stoloniferum, ceratopteris thalictroides, allium sativum and urtica dioica were investigated using immunofluorescence and electron microscopy. arrays of cortical microtubules were observed in all hairs. the microtubules in the hairs show net axial orientations, but in allium and urtica helical microtubule patterns are also present. numerical parameters of microtubules in raphanus, equisetum and lim ...20144066793
evaluation of 31 potential biofumigant brassicaceous plants as hosts for three meloiodogyne species.brassicaceous cover crops can be used for biofumigation after soil incorporation of the mowed crop. this strategy can be used to manage root-knot nematodes (meloidogyne spp.), but the fact that many of these crops are host to root-knot nematodes can result in an undesired nematode population increase during the cultivation of the cover crop. to avoid this, cover crop cultivars that are poor or nonhosts should be selected. in this study, the host status of 31 plants in the family brassicaceae for ...201425276003
enhanced reduction of phenol content and toxicity in olive mill wastewaters by a newly isolated strain of coriolopsis gallica.the search for novel microorganisms able to degrade olive mill wastewaters (omw) and withstand the toxic effects of the initially high phenolic concentrations is of great scientific and industrial interest. in this work, the possibility of reducing the phenolic content of omw using new isolates of fungal strains (coriolopsis gallica, bjerkandera adusta, trametes versicolor, trichoderma citrinoviride, phanerochaete chrysosporium, gloeophyllum trabeum, trametes trogii, and fusarium solani) was inv ...201423979847
toxicity assessment through multiple endpoint bioassays in soils posing environmental risk according to regulatory screening values.toxicity profiles of two soils (a brownfield in legazpi and an abandoned iron mine in zugaztieta; basque country) contaminated with several metals (as, zn, pb and cu in legazpi; zn, pb, cd and cu in zugaztieta) and petroleum hydrocarbons (in legazpi) were determined using a multi-endpoint bioassay approach. investigated soils exceeded screening values (svs) of regulatory policies in force (basque country; europe). acute and chronic toxicity bioassays were conducted with a selected set of test sp ...201424819436
use of green manure crops and sugar beet varieties to control heterodera betae.although it is less studied than the white beet cyst nematode (heterodera schachtii), the yellow beet cyst nematode (h. betae) has been found in many countries in europe. for example in the netherlands, france and spain. h. betae causes yield losses on sandy soils. a high infestation can result in loss of complete plants. in the netherlands, this nematode is especially found in the south eastern and north eastern part, where it occurs on 18% and 5% of the fields, respectively. from a project of ...201426084110
chaetoglobosins from chaetomium globosum, an endophytic fungus in ginkgo biloba, and their phytotoxic and cytotoxic activities.in preceding studies, cultivation of chaetomium globosum, an endophytic fungus in ginkgo biloba, produced five cytochalasan mycotoxins, chaetoglobosins a, g, v, vb, and c (1-5), in three media. in the present work, five known chaetoglobosins, c, e, f, fex, and 20-dihydrochaetoglobosin a (5-9), together with the four known compounds (11-14), were isolated from the meoh extracts of the solid culture of the same endophyte. the structures of these metabolites were elucidated on the basis of spectros ...201424708412
phytotoxicity of 4,8-dihydroxy-1-tetralone isolated from carya cathayensis sarg. to various plant species.the aqueous extract from carya cathayensis sarg. exocarp was centrifuged, filtered, and separated into 11 elution fractions by x-5 macroporous resin chromatography. a phenolic compound, 4,8-dihydroxy-1-tetralone (4,8-dht) was isolated from the fractions with the strongest phytotoxicity by bioassy-guided fractionation, and investigated for phytotoxicity on lettuce (latuca sativa l.), radish (raphanus sativus l.), cucumber (cucumis sativus l.), onion (allium cepa l.) and wheat (triticum aestivum l ...201425264832
a comparative study of the terrestrial ecotoxicity of selected protic and aprotic ionic liquids.ionic liquids (ils) are a fairly new and very promising group of compounds with a vast variety of possible structures and uses. they are considered to be potentially "green", but their impact on the environment tends to be neglected or not studied enough, especially when it comes to terrestrial ecotoxicity, where there are very few studies performed to date. this work presents a comparative study of the terrestrial ecotoxicity of selected representatives of two ils groups: a new family of protic ...201424630250
determination of cadmium, lead and zinc in vegetables in jaipur (india).an atomic absorption spectroscopic method was used for the determination of lead, cadmium and zinc in vegetables grown in and around jaipur food stuffs irrigated with industrial waste water. vegetable samples were collected after maturity, and analyzed, such as spinach (spinacia oleracea), ladyfinger (abelmoschus esulentus), pepper mint (menthe pipereta), brinjal (solanum melongena), coriander (coriandrum sativum), cauliflower (brassica oleracea), onion (allium cepa), radish (raphanus sativus), ...201426445755
chemical composition and biological activities of the essential oil from anredera cordifolia grown in brazil.the chemical composition of the essential oil of anredera cordifolia (ten.) steenis (basellaceae), grown in brazil, was studied by means of gc and gc-ms analysis. in all, 19 compounds were identified, accounting for 91.6% of the total oil; hydrocarbons were the main constituents (67.7%). the essential oil was evaluated for its in vitro potential phytotoxic activity against germination and initial radicle growth of raphanus sativus l., sinapis arvensis l., and phalaris canariensis l. seeds. at 1. ...201425230514
comparative analysis of the radish genome based on a conserved ortholog set (cos) of brassica.this manuscript provides a brassica conserved ortholog set (cos) that can be used as diagnostic cross-species markers as well as tools for genetic mapping and genome comparison of the brassicaceae. a conserved ortholog set (cos) is a collection of genes that are conserved in both sequence and copy number between closely related genomes. cos is a useful resource for developing gene-based markers and is suitable for comparative genome mapping. we developed a cos for brassica based on proteome comp ...201425056003
the xanthomonas campestris effector protein xopdxcc8004 triggers plant disease tolerance by targeting della proteins.plants protect themselves from the harmful effects of pathogens by resistance and tolerance. disease resistance, which eliminates pathogens, can be modulated by bacterial type iii effectors. little is known about whether disease tolerance, which sustains host fitness with a given pathogen burden, is regulated by effectors. here, we examined the effects of the xanthomonas effector protein xopdxcc8004 on plant disease defenses by constructing knockout and complemented xanthomonas strains, and perf ...201425040905
heavy metals bioconcentration from soil to vegetables and assessment of health risk caused by their ingestion.the present study was undertaken to assess the non-carcinogenic human health risk of heavy metals through the ingestion of locally grown and commonly used vegetables viz. raphanus sativus (root vegetable), daucus carota (root vegetable), benincasa hispida (fruit vegetable) and brassica campestris leaves (leafy vegetable) in a semi-urbanized area of haryana state, india. heavy metal quantification of soil and vegetable samples was done using flame atomic absorption spectrophotometer. lead, cadmiu ...201424464601
kazusa marker database: a database for genomics, genetics, and molecular breeding in plants.in order to provide useful genomic information for agronomical plants, we have established a database, the kazusa marker database (http://marker.kazusa.or.jp). this database includes information on dna markers, e.g., ssr and snp markers, genetic linkage maps, and physical maps, that were developed at the kazusa dna research institute. keyword searches for the markers, sequence data used for marker development, and experimental conditions are also available through this database. currently, 10 pl ...201425320561
4-methylthio-butanyl derivatives from the seeds of raphanus sativus and their biological evaluation on anti-inflammatory and antitumor activities.raphanus sativus seeds (brassicaceae) known as raphani semen have long been used as anti-cancer and/or anti-inflammatory agents in korean traditional medicine. this study was designed to isolate the bioactive constituents from the seed extracts of raphanus sativus and evaluate their anti-inflammatory and antitumor activities.201424231071
the evaluation of trace elements of interest in kidney disease in commonly consumed greenhouse vegetables in isfahan, iran: preliminary results.trace elements play a significant role in biological processes. the aim of this study was to evaluate the trace elements of interest in kidney disease in commonly consumed greenhouse vegetables in isfahan, iran. six kinds of greenhouse vegetables namely; raphanus sativus (radish), cucumis sativus (cucamber), solanum lycopersicum (tomato), green capsicum annuum (green bell pepper), yellow capsicum annuum (yellow bell pepper) and red capsicum annuum (red bell pepper) were collected from isfahan gr ...201425340168
toxicity identification evaluation of anaerobically treated swine slurry: a comparison between daphnia magna and raphanus sativus.anaerobic digestion does not efficiently reduce ionic compounds present in swine slurry, which could present a potential risk to aquatic ecosystems (surface runoff) and terrestrial ambient (irrigation). the objective of this study was to evaluate the ecotoxicological characteristics of anaerobically treated swine slurry using acute and chronic (epicotyl elongation) toxicity tests with daphnia magna and raphanus sativus and identification of suspected toxic compounds using the toxicity identifica ...201425190563
spatial distribution of heavy metals in soil, water, and vegetables of farms in sanandaj, kurdistan, iran.heavy metals are ubiquitous elsewhere in nature and their measurement in environment is necessary to develop health management strategies. in this study, we aimed to find out concentrations and spatial patterns of heavy metals in main farms of sanandaj in kurdistan, iran.201425431657
draft sequences of the radish (raphanus sativus l.) genome.radish (raphanus sativus l., n = 9) is one of the major vegetables in asia. since the genomes of brassica and related species including radish underwent genome rearrangement, it is quite difficult to perform functional analysis based on the reported genomic sequence of brassica rapa. therefore, we performed genome sequencing of radish. short reads of genomic sequences of 191.1 gb were obtained by next-generation sequencing (ngs) for a radish inbred line, and 76,592 scaffolds of ≥ 300 bp were con ...201424848699
biotic elicitors effectively increase the glucosinolates content in brassicaceae sprouts.several biotic elicitors have been used in brassicaceae species to enhance their phytochemical quality. however, there is no comparison between elicitors under controlled growth conditions. in order to draw general conclusions about the use of elicitors to enrich ready-to-eat sprouts in health-promoting glucosinolates, the aim of this study was to unveil the effect of the phytohormones methyl jasmonate (25 μm), jasmonic acid (150 μm), and salicylic acid (100 μm), the oligosaccharides glucose (27 ...201424484436
evaluation of zinc oxide nanoparticle toxicity in sludge products applied to agricultural soil using multispecies soil systems.to study the environmental impact of nanoparticles, the sludges of wastewater (wwts) and water treatment (wts) plants enriched with zno nanoparticles were added to agricultural soil, and the toxic effects of the nanoparticles were studied using a microcosm system based on the soil. the wwts treated soils were characterised by statistically significant decreases (p<0.05) in vicia sativa germination at the lowest (76.2%) and medium (95.2%) application rates, decreases in the fresh biomass for trit ...201425194764
single-step purification of peroxidase by 4-aminobenzohydrazide from turkish blackradish and turnip roots.peroxidases (pods) were purified from the turkish blackradish (raphanus sativus l.) (tbr) and turnip (brassica rapa l.) using a simple and effective single-step method. an affinity resin was synthesised by coupling the 4-aminobenzohydrazide ligand and the l-tyrosine spacer-arm to cnbr-activated-sepharose-4b. the purification factors for the tbr-pod and the turnip-pod were 40.3-fold (with a yield of 10.6%) and 269.3-fold (with a yield of 9%), respectively. the molecular masses of the tbr-pod and ...201424360459
characterization of cytochalasins from the endophytic xylaria sp. and their biological functions.bioassay-guided fractionation of the fermentation extract of xylaria sp. xc-16, an endophyte from toona sinensis led to the isolation of two new cytochalasans cytochalasin z27, 1, and cytochalasin z28, 2, along with three known compounds seco-cytochalasin e, 3, and cytochalasin z18, 4, and cytochalasin e, 5. the structures of 1 and 2 were elucidated by spectroscopic and electronic circular dichroism methods. compound 5 was shown to be potently cytotoxic against brine shrimp (lc50 = 2.79 μm), com ...201425350301
perfluoroalkyl acid distribution in various plant compartments of edible crops grown in biosolids-amended soils.crop uptake of perfluoroalkyl acids (pfaas) from biosolids-amended soil has been identified as a potential pathway for pfaa entry into the terrestrial food chain. this study compared the uptake of pfaas in greenhouse-grown radish (raphanus sativus), celery (apium graveolens var. dulce), tomato (lycopersicon lycopersicum), and sugar snap pea (pisum sativum var. macrocarpon) from an industrially impacted biosolids-amended soil, a municipal biosolids-amended soil, and a control soil. individual con ...201424918303
complete mitochondrial genome of eruca sativa mill. (garden rocket).eruca sativa (cruciferae family) is an ancient crop of great economic and agronomic importance. here, the complete mitochondrial genome of eruca sativa was sequenced and annotated. the circular molecule is 247,696 bp long, with a g+c content of 45.07%, containing 33 protein-coding genes, three rrna genes, and 18 trna genes. the eruca sativa mitochondrial genome may be divided into six master circles and four subgenomic molecules via three pairwise large repeats, resulting in a more dynamic struc ...201425157569
de novo assembly and characterization of the complete chloroplast genome of radish (raphanus sativus l.).radish (raphanus sativus l.) is an edible root vegetable crop that is cultivated worldwide and whose genome has been sequenced. here we report the complete nucleotide sequence of the radish cultivar wk10039 chloroplast (cp) genome, along with a de novo assembly strategy using whole genome shotgun sequence reads obtained by next generation sequencing. the radish cp genome is 153,368 bp in length and has a typical quadripartite structure, composed of a pair of inverted repeat regions (26,217 bp ea ...201425151309
chemical composition and biological activity of the essential oil of origanum vulgare ssp. hirtum from different areas in the southern apennines (italy).the chemical composition of the essential oils of origanum vulgare ssp. hirtum, growing wild in three different localities in the southern apennines, was studied by gc-fid and gc/ms analyses. in total, 103 compounds were identified. the oils were mainly composed of phenolic compounds and all oils belonged to the chemotype carvacrol/thymol. the three essential oils were evaluated for their in vitro phytotoxic activity by determining their influence on the germination and initial radicle elongatio ...201424706631
bioactive metabolites isolated from penicillium sp. yy-20, the endophytic fungus from ginkgo biloba.six known metabolites, adenosine (1), methyl β-d-ribofuranoside (2), adenine (3), 2'-deoxyadenosine (4), 3-methylpiperazine-2,5-dione (5) and 2'-deoxyuridine (6), were isolated from the extracts of the endophytic fungus penicillium sp. yy-20 isolated from the root of ginkgo biloba, and their structures were elucidated by spectroscopic methods. the antioxidant and growth-promoting activities of these compounds were first evaluated. the results indicated that compounds 1, 3 and 4 exhibited potenti ...201424144081
rare earth elements (rees): effects on germination and growth of selected crop and native plant species.the phytotoxicity of rare earth elements (rees) is still poorly understood. the exposure-response relationships of three native canadian plant species (common milkweed, asclepias syriaca l., showy ticktrefoil, desmodium canadense (l.) dc. and switchgrass, panicum virgatum l.) and two commonly used crop species (radish, raphanus sativus l., and tomato, solanum lycopersicum l.) to the rees lanthanum (la), yttrium (y) and cerium (ce) were tested. in separate experiments, seven to eight doses of eac ...201423978671
the c-terminal extension peptide of non-photoconvertible water-soluble chlorophyll-binding proteins (class ii wscps) affects their solubility and stability: comparative analyses of the biochemical and chlorophyll-binding properties of recombinant brassica, raphanus and lepidium wscps with or without their c-terminal extension peptides.numerous members of the brassicaceae possess non-photoconvertible water-soluble chlorophyll (chl)-binding proteins (class ii wscps), which function as chl scavengers during cell disruption caused by wounding, pest/pathogen attacks, and/or environmental stress. class ii wscps have two extension peptides, one at the n-terminus and one at the c-terminus. the n-terminal peptide acts as a signal peptide, targeting the protein to the endoplasmic reticulum body, a unique defensive organelle found only ...201424389799
organelle simple sequence repeat markers help to distinguish carpelloid stamen and normal cytoplasmic male sterile sources in broccoli.we previously discovered carpelloid stamens when breeding cytoplasmic male sterile lines in broccoli (brassica oleracea var. italica). in this study, hybrids and multiple backcrosses were produced from different cytoplasmic male sterile carpelloid stamen sources and maintainer lines. carpelloid stamens caused dysplasia of the flower structure and led to hooked or coiled siliques with poor seed setting, which were inherited in a maternal fashion. using four distinct carpelloid stamens and twelve ...201526407159
amiprophosmethyl-induced efficient in vitro production of polyploids in raphanobrassica with the aid of aminoethoxyvinylglycine (avg) in the culture medium.optimum conditions for obtaining tetraploid were investigated in raphanobrassica, the intergeneric hybrid between radish (raphanus sativus) and kale (brassica oleracea var. acephala) by treating in vitro plants with an anti-mitotic agent, amiprophosmethyl (apm). initially, no tetraploids but hexaploids and octaploids were induced by the treatments. although the leaves of these polyploids of raphanobrassica showed chlorosis during subcultures in in vitro conditions, the chlorosis could be success ...201526719742
uptake and effects of six rare earth elements (rees) on selected native and crop species growing in contaminated soils.rare earth elements (rees) have become increasingly important metals used in modern technology. processes including mining, oil refining, discarding of obsolete equipment containing rees, and the use of ree-containing phosphate fertilizers may increase the likelihood of environmental contamination. however, there is a scarcity of information on the toxicity and accumulation of these metals to terrestrial primary producers in contaminated soils. the objective of this work was to assess the phytot ...201526076480
essential oil composition and antigermination activity of artemisia dracunculus (tarragon).the chemical composition of an italian oil of tarragon (artemisia dracunculus l.) was analyzed by gc/eims. the major compound of the oil was estragole (73.3%), followed by limonene (5.4%), (e)-β-ocimene (5.3%), β-pinene (3.4%) and (z)-β-ocimene (3.0%). the essential oil was tested "in vitro" for its antigermination activity against raphanus sativus l., lepidium sativum l, papaver rhoeas l. and avena fatua l. seeds and demonstrating a good inhibitory activity in a dose-dependent way.201526434144
potential health concerns of trace elements and mineral content in commonly consumed greenhouse vegetables in isfahan, iran.this study aimed to investigate the potential health concerns of trace elements and mineral content of commonly consumed greenhouse vegetables in isfahan, iran.201526605243
antimicrobial and allelopathic metabolites produced by penicillium brasilianum.six known compounds, isoroquefortine c (1), griseofulvin (2), ergosterol peroxide (3), 3β-hydroxy-(22e,24r)-ergosta-5,8,22-trien-7-one (4), cerevisterol (5) and (22e,24r)-6β-methoxyergosta-7,22-diene-3β,5α-diol (6), were produced by the fungus penicillium brasilianum, and their structures were elucidated by spectroscopic methods. this is the first report on isoroquefortine c as naturally occurring compound. their bioactivities against five phytopathogenic fungi (gibeberalla saubinetti, fusarium ...201525103127
progesterone 5β-reductase genes of the brassicaceae family as function-associated molecular markers.this study aimed to define progesterone 5β-reductases (p5βr, ec 1.3.99.6, enone 1,4-reductases) as function-associated molecular markers at the plant family level. therefore cdnas were isolated from 25 brassicaceae species, including two species, erysimum crepidifolium and draba aizoides, known to produce cardiac glycosides. the sequences were used in a molecular phylogeny study. the cladogram created is congruent to the existing molecular analyses. recombinant his-tagged forms of the p5βr cdnas ...201526108256
iscr plays a role in oxidative stress resistance and pathogenicity of a plant pathogen, xanthomonas campestris.iron-sulfur ([fe-s]) cluster is an essential cofactor of proteins involved in various physiological processes including cellular defense against oxidative stress. in xanthomonas campestris pv. campestris (xcc), iscr plays a negative role in regulation of the transcription of [fe-s] assembly genes, iscr-sufbcds. the expression level of sufbcds was up-regulated in an xcc iscr mutant. in addition, the iscr promoter activity in an xcc iscr mutant was also higher than the wild-type strain, indicating ...201525200360
construction of a reference genetic map of raphanus sativus based on genotyping by whole-genome resequencing.this manuscript provides a genetic map of raphanus sativus that has been used as a reference genetic map for an ongoing genome sequencing project. the map was constructed based on genotyping by whole-genome resequencing of mapping parents and f 2 population. raphanus sativus is an annual vegetable crop species of the brassicaceae family and is one of the key plants in the seed industry, especially in east asia. assessment of the r. sativus genome provides fundamental resources for crop improveme ...201525403353
methyl jasmonate affects morphology, number and activity of endoplasmic reticulum bodies in raphanus sativus root cells.the endoplasmic reticulum (er) bodies are er-derived structures that are found in brassicaceae species and thought to play a role in defense. here, we have investigated the occurrence, distribution and function of er bodies in root cells of raphanus sativus using a combination of microscopic and biochemical methods. we have also assessed the response of er bodies to methyl jasmonate (meja), a phytohormone that mediates plant defense against wounding and pathogens. our results show that (i) er bo ...201525305245
cadmium, copper, and lead accumulation and bioconcentration in the vegetative and reproductive organs of raphanus sativus: implications for plant performance and pollination.several studies have found high levels of cadmium (cd), copper (cu), and lead (pb) in honey bee hives located near urbanized or industrial areas. insect herbivores and pollinators may come in contact with environmental contaminants in the leaves and flowers they forage upon in these areas. our study quantified which of these metals are accumulated in the tissues of a common weedy plant that can serve as a route of exposure for insects. we grew raphanus sativus (crop radish) in semi-hydroponic sa ...201525845355
traditional phytoremedies for the treatment of menstrual disorders in district udhampur, j&k, india.herbal remedies form an integral part of healing and are considered to be the oldest forms of health care known to mankind. the present study aims to document traditional phytoremedies for the treatment of menstrual disorders in udhampur district of j&k, india.201525479156
scenario-targeted toxicity assessment through multiple endpoint bioassays in a soil posing unacceptable environmental risk according to regulatory screening values.lanestosa is a chronically polluted site (derelict mine) where the soil (lanestosa (la) soil) exceeds screening values (svs) of regulatory policies in force (basque country; europe) for zn, pb and cd. a scenario-targeted toxicity assessment was carried out on the basis of a multi-endpoint bioassay approach. acute and chronic toxicity bioassays were conducted with selected test species (vibrio fischeri, dictyostelium discoideum, lactuca sativa, raphanus sativus and eisenia fetida) in combination ...201525940475
water-soluble chlorophyll-binding proteins from arabidopsis thaliana and raphanus sativus target the endoplasmic reticulum body.non-photosynthetic chlorophyll (chl) proteins called water-soluble chl-binding proteins are distributed in brassicaceae plants. brassica oleracea wscp (bowscp) and lepidium virginicum wscp (lvwscp) are highly expressed in leaves and stems, while arabidopsis thaliana wscp (atwscp) and raphanus sativus wscp (rshwscp) are highly transcribed in floral organs. bowscp and lvwscp exist in the endoplasmic reticulum (er) body. however, the subcellular localization of atwscp and rshwscp is still unclear. ...201526289422
biofumigation with brassica juncea, raphanus sativus and eruca sativa for the management of field populations of the potato cyst nematode globodera pallida.the viability of potato cyst nematode (pcn) populations (globodera pallida) was evaluated in three field experiments using brassica juncea, raphanus sativus and eruca sativa amendments. these species were summer cultivated and autumn incorporated in experiment 1; in experiment 2, overwintered brassicaceous cover crops were spring incorporated. experiment 3 involved determination of effects of metconazole application on biomass/glucosinolate production by b. juncea and r. sativus and on pcn pre- ...201524965697
identification of a new antibacterial sulfur compound from raphanus sativus seeds.raphanus sativus l. (radish), a member of brassicaceae, is widely used in traditional medicine in various cultures for treatment of several diseases and disorders associated with microbial infections. the antibacterial activity of the different plant parts has been mainly attributed to several isothiocyanate (itc) compounds. however, the low correlation between the itc content and antibacterial activity suggests the involvement of other unknown compounds. the objective of this study was to inves ...201627781070
characterization of a digestive α-amylase in the midgut of pieris brassicae l. (lepidoptera: pieridae).the current study deals with a digestive α-amylase in the larvae of pieris brassicae l. through purification, enzymatic characterization, gene expression, and in vivo effect of a specific inhibitor, acarbose. although α-amylase activity was the highest in the whole gut homogenate of larvae but compartmentalization of amylolytic activity showed an equal activity in posterior midgut (pm) and anterior midgut (am). a three step purification using ammonium sulfate, sepharyl g-100 and deae-cellulose f ...201627014094
enhanced resistance to sclerotinia sclerotiorum in brassica napus by co-expression of defensin and chimeric chitinase genes.sclerotinia stem rot caused by sclerotinia sclerotiorum is one of the major fungal diseases of brassica napus l. to develop resistance against this fungal disease, the defensin gene from raphanus sativus and chimeric chit42 from trichoderma atroviride with a c-terminal fused chitin-binding domain from serratia marcescens were co-expressed in canola via agrobacterium-mediated transformation. twenty transformants were confirmed to carry the two transgenes as detected by polymerase chain reaction ( ...201626862081
molecular characterization of the complete genome of three basal-br isolates of turnip mosaic virus infecting raphanus sativus in china.turnip mosaic virus (tumv) infects crops of plant species in the family brassicaceae worldwide. tumv isolates were clustered to five lineages corresponding to basal-b, basal-br, asian-br, world-b and oms. here, we determined the complete genome sequences of three tumv basal-br isolates infecting radish from shandong and jilin provinces in china. their genomes were all composed of 9833 nucleotides, excluding the 3'-terminal poly(a) tail. they contained two open reading frames (orfs), with the lar ...201627271614
comparison of helper component-protease rna silencing suppression activity, subcellular localization, and aggregation of three korean isolates of turnip mosaic virus.in 2014, we performed a nationwide survey in korean radish fields to investigate the distribution and variability of turnip mosaic virus (tumv). brassica rapa ssp. pekinensis sap-inoculated with three isolates of tumv from infected radish tissue showed different symptom severities, whereas symptoms in raphanus sativus were similar for each isolate. the helper component-protease (hc-pro) genes of each isolate were sequenced, and phylogenetic analysis showed that the three korean isolates were clu ...201627059238
seed-borne viral dsrna elements in three cultivated raphanus and brassica plants suggest three cryptoviruses.since the 1970s, several dsrna viruses, including radish yellow edge virus, raphanus sativus virus 1, raphanus sativus virus 2, and raphanus sativus virus 3, have been identified and reported as infecting radish. in the present study, in conjunction with a survey of seed-borne viruses in cultivated brassica and raphanus using the dsrna diagnostic method, we discovered 3 novel cryptoviruses that infect brassica and raphanus: raphanus sativus partitivirus 1, which infects radish (raphanus sativus) ...201626974503
inducible expression of the de-novo designed antimicrobial peptide sp1-1 in tomato confers resistance to xanthomonas campestris pv. vesicatoria.antimicrobial peptides (amps) are small peptides with less than 50 amino acids and are part of the innate immune response in almost all organisms, including bacteria, vertebrates, invertebrates and plants. amps are active against a broad-spectrum of pathogens. the inducible expression of amps in plants is a promising approach to combat plant pathogens with minimal negative side effects, such as phytotoxicity or infertility. in this study, inducible expression of the de-novo designed amp sp1-1 in ...201627706237
differences in stability of seed-associated microbial assemblages in response to invasion by phytopathogenic microorganisms.seeds are involved in the vertical transmission of microorganisms from one plant generation to another and consequently act as reservoirs for the plant microbiota. however, little is known about the structure of seed-associated microbial assemblages and the regulators of assemblage structure. in this work, we have assessed the response of seed-associated microbial assemblages of raphanus sativus to invading phytopathogenic agents, the bacterial strain xanthomonas campestris pv. campestris (xcc) ...201627077013
identification, expression, and functional analysis of cle genes in radish (raphanus sativus l.) storage root.radish (raphanus sativus l.) is a widespread agricultural plant forming storage root due to extensive secondary growth which involves cambium proliferation and differentiation of secondary conductive tissues. closely related to the model object arabidopsis thaliana, radish is a suitable model for studying processes of secondary growth and storage root development. cle peptides are a group of peptide phytohormones which play important role in the regulation of primary meristems such as sam, ram, ...201626821718
myb transcription factor isolated from raphanus sativus enhances anthocyanin accumulation in chrysanthemum cultivars.a myb transcription factor gene, rsmyb1, from radish was introduced into the chrysanthemum cultivars 'peach nd', 'peach red', and 'vivid scarlet' under the control of the cauliflower mosaic virus 35s promoter. presence of rsmyb1 in transgenic lines was confirmed using polymerase chain reaction (pcr). results of reverse-transcription-pcr analysis revealed that the expression of rsmyb1 was stable in all transgenic lines and could enhance the expression levels of three key biosynthetic genes (f3h, ...201628330149
bio-optimization of the carbon-to-nitrogen ratio for efficient vermicomposting of chicken manure and waste paper using eisenia fetida.the main objective of the present study was to determine the optimum c/n ratio for converting waste paper and chicken manure to nutrient-rich manure with minimum toxicity. six treatments of c/n ratio 20, 30, 40, 50, 60, and 70 (t1, t2, t3, t4, t5, and t6, respectively) achieved by mixing chicken manure with shredded paper were used. the study involved a composting stage for 20 days followed by vermicomposting with eisenia fetida for 7 weeks. the results revealed that 20 days of composting consid ...201627197657
enantioselective separation of 4,8-dht and phytotoxicity of the enantiomers on various plant species.as a candidate for bioherbicide, 4,8-dihydroxy-1-tetralone (4,8-dht) was isolated from caryospora callicarpa epicarp and its two enantiomers, s-(+)-isosclerone and r-(-)-regiolone, were separated by chiral high-performance liquid chromatography (hplc) on a chiralcel od column with chiral stationary phase (csp)-coated cellulose-tris(3,5-dimethylphenylcarbamate). then, the phytotoxicity of 4,8-dht and its enantiomers toward the seeds germination and seedling growth of the five tested plant species ...201627110760
genome-wide characterization of the mads-box gene family in radish (raphanus sativus l.) and assessment of its roles in flowering and floral organogenesis.the mads-box gene family is an important transcription factor (tf) family that is involved in various aspects of plant growth and development, especially flowering time and floral organogenesis. although it has been reported in many plant species, the systematic identification and characterization of mads-box tf family is still limited in radish (raphanus sativus l.). in the present study, a comprehensive analysis of mads-box genes was performed, and a total of 144 mads-box family members were i ...201627703461
development of phytotoxicity indexes and their correlation with ecotoxicological, stability and physicochemical parameters during passive composting of poultry manure.both raw and composted poultry manure is applied as soil amendment. the aims of this study were: (1) to develop phytotoxicity indexes for organic wastes and composts, and (2) to assess the correlation among phytotoxicity indexes, ecotoxicological endpoints and stability and physicochemical parameters during passive composting of poultry manure. six 2-m(3) composting piles were constructed and four parameter groups (physicochemical and microbiological parameters, ecotoxicological endpoints, and b ...201627185192
changes in the ecological properties of organic wastes during their biological treatment.organic wastes, such as the organic fractions of municipal solid waste (ofmsw) or sewage sludge (ss), have become a serious environmental problem in russia as well as in other countries. the use of these wastes as soil amendments allows their negative impact on the environment to be minimized. however, before these wastes can be used, they need to be treated appropriately in order to decrease their level of hazard. in this study, composting of raw ss, ofmsw, a mixture of these two wastes (ofmsw+ ...201627692790
the effect of new thiophene-derived aminophosphonic derivatives on growth of terrestrial plants: a seedling emergence and growth test.the aim of this work was to synthesize selected thiophene-derived aminophosphonic systems and evaluate the phytotoxicity of newly obtained products according to the oecd 208 guideline. seven new thiophene-derived n-substituted dimethyl aminomethylphosphonic acid esters 2a-h were synthesized by the addition of an appropriate phosphite to azomethine bond of starting schiff bases 1a-h, and nmr spectroscopic properties of aminophosphonates were investigated. these eight compounds were analyzed in re ...201627248990
evolution of mustard (brassica juncea coss) subspecies in china: evidence from the chalcone synthase gene.to explore the phylogenetic relationship, genome donor, and evolutionary history of the polyploid mustard (brassica juncea) from china, eighty-one sequences of the chalcone synthase gene (chs) were analyzed in 43 individuals, including 34 b. juncea, 2 b. rapa, 1 b. nigra, 2 b. oleracea, 1 b. napus, 1 b. carinata, and 2 raphanus sativus. a maximum likelihood analysis showed that sequences from b. juncea were separated into two well-supported groups in accordance with the a and b genomes, whereas ...201627173323
arsenic accumulation in brassicaceae seedlings and its effects on growth and plant anatomy.we wished to evaluate the effects of arsenic on the morphology and anatomy of brassica oleracea, raphanus sativus, brassica juncea, brassica oleracea var. capitata and brassica oleracea var. italica. seeds were subjected to concentrations 0µm, 250µm, 350µm and 450µm arsenic in the form of sodium arsenate (na2haso4·7h2o) during 12 days. all species accumulated more arsenic in the roots than in the shoots, except for b. oleracea var. capitata. there was no difference of translocation factor betwee ...201626435391
chemical composition, antibacterial and phytotoxic activities of peganum harmala seed essential oils from five different localities in northern africa.peganum harmala l., also known as syrian rue or pègano, is a herbaceous plant belonging to the zygohpyllaceae family, and is widely used in traditional medicine. the chemical composition of essential oils of p. harmala seeds from five different regions of northern africa (algeria, egypt, libya, morocco and tunisia) was studied by gc and gc-ms analyses. a total of 105 compounds were identified, the main components being oxygenated monoterpenes and oxygenated sesquiterpenes. eugenol is the main co ...201627649128
environmental surveillance of commonly-grown vegetables for investigating potential lead and chromium contamination intensification in bangladesh.with regard to previously reported lead (pb) and chromium (cr) contamination in egg, a semi-quantitative assessment of the general environment of bangladesh is carried out through nation-wide sampling of commonly grown and consumed vegetables. five vegetables, namely, white potato (solanum tuberosum), green cabbage (brassica oleracea capitata var. alba l.), red spinach (amaranthus dubius), white radish (raphanus sativus var. longipinnatus), and green bean (phaseolus vulgaris) were selected for s ...201627812443
development of male sterile eruca sativa carrying a raphanus sativus/brassica oleracea cybrid cytoplasm.alloplasmic male sterile breeding lines of eruca sativa were developed by intergeneric hybridization with cms- brassica oleracea, followed by recurrent backcrosses and determination of the breeding value.201626602232
characterization of rsmyb28 and rsmyb29 transcription factor genes in radish (raphanus sativus l.).glucosinolates (gsls) are important secondary metabolites in brassicaceae plants. previous studies have mainly focused on gsl contents, types, and biosynthesis-related genes, but the molecular characterization patterns of gsl biosynthesis-related transcription factors remain largely unexplored in radish (raphanus sativus l.). to isolate transcription factor genes regulating the gsl biosynthesis, genomic dna and cdna sequences of rsmyb28 and rsmyb29 genes were isolated in radish. two r2r3-myb dom ...201627706769
evaluation of phytotoxicity effect on selected crops using treated and untreated wastewater from different configurative domestic wastewater plants.this study investigated the phytotoxicity effect of untreated and treated wastewater collected from two different configurations of domestic wastewater treatment plants in south africa. the phytotoxicity effect on vegetable seed growth was studied in terms of germination index (gi), relative seed germination (rsg) and relative root elongation (rre) using four commercial crop varieties, viz., tomato (lycopersicon esculentum), radish (raphanus sativus), carrot (daucus carota) and onion (allium cep ...201626806819
in situ identification of labile precursor compounds and their short-lived intermediates in plants using in vivo nanospray high-resolution mass spectrometry.many secondary metabolites in plants are labile compounds which under environmental stress, are difficult to detect and track due to the lack of rapid in situ identification techniques, making plant metabolomics research difficult. therefore, developing a reliable analytical method for rapid in situ identification of labile compounds and their short-lived intermediates in plants is of great importance.201627313155
metabolic activity of radish sprouts derived isothiocyanates in drosophila melanogaster.we used drosophila melanogaster as a model system to study the absorption, metabolism and potential health benefits of plant bioactives derived from radish sprouts (raphanus sativus cv. rambo), a brassicaceae species rich in glucosinolates and other phytochemicals. flies were subjected to a diet supplemented with lyophilized radish sprouts (10.6 g/l) for 10 days, containing high amounts of glucoraphenin and glucoraphasatin, which can be hydrolyzed by myrosinase to the isothiocyanates sulforaphen ...201626901196
co-pigmentation of pelargonidin derivatives in strawberry and red radish model solutions by the addition of phenolic fractions from mango peels.pelargonidin-based colors suffer from notorious instability. a phenolic mango peel extract and defined phenolic fractions thereof were shown to effectively modulate the visible absorption of anthocyanins from strawberry (fragaria x ananassa duch.) and red radish (raphanus sativus l.) by intermolecular co-pigmentation. consistently, non-acylated pelargonidin derivatives from strawberry exerted significantly greater hyper- and bathochromic spectral shifts than their acylated counterparts from red ...201627451227
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