Publications

late features of toxic syndrome due to denatured rapeseed oil.		1982	6120328
pathology of a new toxic syndrome caused by ingestion of adulterated oil in spain.	the toxic syndrome (ts) caused by ingestion of adulterated rapeseed oil in spain is a new disease of multisystemic character whose aetiology and pathogenesis remains unknown. the most prominent pathological feature is a peculiar non-necrotizing vasculitis, that affects mainly the intima and involves vessels of every type and size in practically every organ. the ts begins with an acute clinical picture with pleuropneumopathy, fever, headaches, exanthems and eosinophilia. in these early clinical p ...	1982	7157666
influence of diet on the effects of selenium in the genesis of mammary tumors.	two experiments were conducted to study the interaction of various diets with selenium in the genesis of mammary tumors. in the first experiment, virgin c3h mice were fed either an oregon state university (o.s.u.) chow or this chow plus 0.5 or 2.0 ppm selenium in the drinking water, or wayne lab-blox chow with or without 2.0 ppm selenium in the water. selenium significantly reduced the tumor incidence in mice fed the o.s.u. chow, but not in those fed the wayne chow. in the second experiment, vir ...	1982	7145723
somatic embryogenesis in quite a direct way in cultures of mesophyll protoplasts of brassica napus l.	somatic embryos and plantlets have been obtained in quite a direct way from mesophyll protoplasts isolated from androgenetic plants of two cultivars ("loras" and "tower") of brassica napus. the procedure consists of four steps. proembryos were induced in a medium supplemented with a cytokinin and an auxin at comparatively high concentrations. they developed to mature embryos when the auxin was reduced or omitted.	1982	24257710
comparative macromolecular analysis of the cytoplasms of normal and cytoplasmic male sterile brassica napus.	chloroplast (cp) and mitochondrial (mt) compartments of normal (n) and cytoplasmic male sterile (cms) lines of brassica napus have been characterized and compared on the basis of cp and mt dna restriction enzyme analysis and in vitro protein synthesis by isolated mitochondria. cytoplasmic male sterility of b. napus (rape) comes from cms raphanus sativus (radish) through intergeneric crosses.cp dnas isolated from n and cms lines had distinct restriction patterns with sal i, kpn i and sma i enzyme ...	1982	24270618
anther culture as a breeding tool in rape : ii. progeny analyses of androgenetic lines and induced mutants from haploid cultures.	progeny analysis of androgenetic plants from inbred rape-seed (brassica napus) shows that selective growth of microspores can occur in cultured anthers. the property of privileged growth in culture seems to be linked to such characters as flowering time and seed glucosinolate content which can be analyzed in regenerated plants. this type of selection and the fact that more variability is visible in regenerants from different microspores than in the progeny of the highly inbred anther donor line, ...	1982	24270433
resistance responses to phoma lingam of plants regenerated from selected cell and embryogenic cultures of haploid brassica napus.	resistant plants and plants with reduced susceptibility against the pathogen phoma lingam could be regenerated from selected callus and embryogenic cultures of haploid rape (brassica napus) previously treated with mutagens. in the two in vitro selection systems used - absence of fungus growth on the cultures after incubation with parasite spores and resistance to the toxic filtrate - the resistance to the toxin was effective. in addition, some regenerants with increased tolerance were obtained f ...	1982	24270428
metabolism of long-chain alcohols in cell suspension cultures of soya and rape.	heterotrophic cell suspension cultures of soya (glycine max) and photomixotrophic cell suspension cultures of rape (brassica napus) were incubated with cis-9-[1-(14)c]octadecenol for 3-48 h. it was found that under aerobic conditions large proportions of the alcohol are oxidized to oleic acid, which is incorporated predominantly into phospholipids, whereas up to 30% of the substrate is esterified to wax esters. this is true for both the heterotrophic and the photomixotrophic cell suspension cult ...	1982	24271771
non-zygotic embryos of brassica napus l. contain embryo-specific storage proteins.	the storage-protein content of non-zygotic and zygotic embryos of b. napus was compared, using antibodies to guantitate 12s storage protein in extracts by rocket immunoelectrophoresis. non-zygotic embryos were induced from microspores in anther culture and on the hypocotyls of zygotic embryos in culture. all embryo-like structures were found to contain 12s storage protein, whereas preculture anthers, anthers from which embryos had been removed, and regenerated shoots did not have detectable 12s ...	1982	24272730
influence of canola hulls on gain in weight of larvae of the yellow mealworm, tenebrio molitor l.	larvae of the yellow mealworm, tenebrio molitor l., gembloux strain, race f, were reared on diets in which the protein component was supplied by defatted ground seed, defatted ground dehulled fraction, or defatted ground hulls of brassica napus l. cv. tower or brassica campestris l. cv. candle, obtained from autoclaved seed. they were also fed casein diets to which defatted ground hulls of tower or candle seed were added. gain in weight was equally good for all diets containing candle seed fract ...	1983	6204614
the use of radiography in studies of plant development in vivo.	the growth and development of individual pods in plants of oil-seed rape (brassica napus l.) were followed from anthesis onwards using a soft x-radiation in-vivo technique. changes in the length and width of pods and in the positions, numbers and sizes of seeds within them were determined quantitatively from x-ray plates and photographs. the technique provides a valuable and as yet unexploited tool for studies of plant development.	1983	24258301
formation of complex ether lipids from 1-o-alkylglycerols in cell suspension cultures of rape.	photomixotrophic cell suspension cultures of rape, brassica napus, were incubated with rac-1-o-[1'-(14)c]hexadecylglycerol. radioactivity was incorporated predominantly into choline glycerophospholipids. prolonged incubation led also to considerable proportions of labeled ethanolamine glycerophospholipids. in addition to these ionic lipids,isomeric hexadecylacylglycerols as well as hexadecyldiacylglycerols were formed. about a third of the hexadecylglycerol supplied as substrate was cleaved with ...	1983	24264539
physical and gene mapping of chloroplast dna from normal and cytoplasmic male sterile (radish cytoplasm) lines of brassica napus.	using the restriction endonucleases saii, smai, bgii and kpni, physical maps of chloroplast dna isolated from normal and cytoplasmic male sterile (radish cytoplasm) lines of b. napus were constructed and compared. in this study, a rapid and simple procedure was developed for the isolation of chloroplast dna restriction fragments from low gelling temperature agarose gels.the overall structural organization of n and cms b. napus appears to be rather similar to that of cpdnas of other higher plants ...	1983	24173113
two dimensional analysis of chloroplast proteins from normal and cytoplasmic male sterile brassica napus.	stromal and thylakoid proteins isolated from normal (n) and cytoplasmic male sterile (cms) lines of brassica napus have been compared using a two dimensional gel separation. it has been shown that: 1) stromal compartments of the two lines were very similar; 2) although there was extensive homology between protein maps of thylakoids isolated from the two lines, these could be distinguished by the spots corresponding to the β subunits of the coupling factor cf1 from the atpase complex.	1983	24264952
cdna clones for brassica napus seed storage proteins: evidence from nucleotide sequence analysis that both subunits of napin are cleaved from a precursor polypeptide.	napins are a family of small, basic storage proteins synthesized in brassica napus (rapeseed) embryos during seed maturation. cultured embryos also synthesize napins but require exogenous abscisic acid (aba) to maintain high accumulation rates. we synthesized cdna from total rna of embryos cultured on a medium containing aba, and cloned it into the pst1 site of pbr322. two clones containing napin cdna sequences selected by differential colony hybridization using [32p]cdna probes from embryos gro ...	1983	6689334
neuropathological studies on the toxic syndrome related to adulterated rapeseed oil in spain.	biopsies of muscle and sural nerves, and autopsies of patients affected by the toxic and neuromuscular syndrome produced by ingestion of adulterated rapeseed oil were studied using morphological, histochemical and ultrastructural methods. in muscle, two pathological pictures were distinguished according to their temporal sequence. in the early phase, the neuromuscular syndrome was characterized by myalgia and an inflammatory infiltration of the perimysium, the capsules of muscle spindles and int ...	1983	6317138
pulmonary vascular lesions in the toxic oil syndrome in spain.	a histological study was made of pulmonary arteries at the necropsies of nine patients who died after the ingestion of denatured rapeseed oil during the epidemic which occurred in spain in may 1981. lesions found in the elastic pulmonary arteries were characterised by pronounced intimal proliferation of an oedematous nature, accumulation of large vacuolated cells within the media, and loss of vascular smooth muscle. in muscular pulmonary arteries there was pronounced medial hypertrophy and intim ...	1983	6648850
osteoarticular manifestations of exposure to toxic rapeseed oil.		1983	6615575
ards due to ingestion of denatured rapeseed oil.	among the different etiologies of noncardiogenic acute pulmonary edema is found the administration or ingestion of various substances. we have studied two patients with ards secondary to the ingestion of toxic oil. both patients presented similar roentgenographic findings characterized by interstitial and alveolar infiltration in "butterfly" distribution, pleural effusion, and a normal cardiac silhouette. the two cases also showed a pulmonary compliance either normal or slightly diminished. with ...	1983	6872595
chloroplast dna evolution and the origin of amphidiploid brassica species.	the origin and evolution of a hybrid species complex in the genus brassica (cabbage, turnip, mustard, rapeseed oil) has been explored through mutational analysis of the maternally inherited chloroplast genome. a detailed chloroplast dna phylogeny enables identification of the maternal parent for most of the amphidiploids examined and permits quantitative resolution of the relative time of hybridization as well as the relative divergence of the diploid parents. contradictory chloroplast and nucle ...	1983	24263412
an epidemic of pulmonary hypertension after toxic rapeseed oil ingestion in spain.	the cardiac profile of 38 patients readmitted to the hospital with the clinical and radiologic findings of pulmonary artery hypertension and right ventricular failure 2 months after ingestion of toxic rapeseed oil was determined with m-mode and two-dimensional echocardiography, pulsed doppler flow studies and right and left heart catheterization and ventriculography. the echocardiogram and pulsed doppler recordings revealed right ventricular enlargement in 84% of the patients, indirect evidence ...	1983	6833663
hydrolytic enzyme activities, mainly from lysosomal localization, in sera from patients who ingested a toxic oil.	some hydrolytic enzyme activities, mainly typical of lysosomal localization, have been determined in blood sera from patients who ingested a rapeseed oil (denatured with anilines and treated by a thermal process), and in healthy subjects. beta-n-acetylglucosaminidase, beta-d-glucosidase, beta-d-glucuronidase, alpha-l-fucosidase and leucine aminopeptidase activities were significantly higher when compared with controls (p less than 0.001); higher activities but not significant (p less than 0.2) d ...	1983	6839504
evidence for a negative modulating effect of erucic acid on the peroxisomal beta-oxidation enzyme system and biogenesis in rat liver.	in male rats fed a 30 cal% high-erucic acid (22:1 (13) (cis] rapeseed oil diet for 4 weeks a transient, small increase in peroxisomal beta-oxidation was observed in liver homogenates and isolated peroxisomes after approximately 1 week. morphometric analysis revealed a progressive decrease in the average size of the liver peroxisomes (by approx. 20%), as well as their volume fraction (by more than 40%). a negative dose-response was observed when peroxisomal beta-oxidation was determined in animal ...	1983	6615876
voluntary food intake of growing pigs given diets containing rapeseed meal, from different types and varieties of rape, as the only protein supplement.	the voluntary food intake, during 30 min periods after morning and afternoon feeds and during 24 h, by growing pigs given diets containing rapeseed meal (rsm) or soya-bean meal (sbm) as the only protein supplement was determined. one diet was offered at a time and a daily changeover sequence of feeding was followed. four rapeseed meals were compared, one from seeds of british-grown winter brassica napus varieties (brsm) and the others from seeds of the varieties tower (trsm), erglu (ersm) and sp ...	1983	6639926
nadph-diaphorase used to estimate alterations in the toxic rapeseed oil syndrome.	the evaluation of nadph-diaphorase in the post-mitochondrial fraction of rat liver has been shown a sensitive method to estimate alterations of microsomal oxidase systems in acute and chronic intoxications. it is also useful to demonstrate the interaction of drugs with barbiturates, which normally induce this enzyme. to study the biochemical liver damage which may occur in the toxic rapeseed oil syndrome, we measured nadph-diaphorase in the post-mitochondrial fraction of rats given oral doses of ...	1983	6659303
effect of dietary supplementation with stearic acid on the severity of myocardial lesions.	histological studies were conducted on ventricular myocardium obtained from male rats of the sprague-dawley strain after 16 weeks of feeding diets containing 20 per cent (w/w) soybean oil, low erucic acid rapeseed oil or high erucic acid rapeseed oil. additional groups of rats were fed similar diets supplemented with up to 8 g/kg of tristearin. increasing dietary intake of stearic acid normalised the plasma ratio of c16:0 to c18:0 for rapeseed oil treatments to ratios analogous to that observed ...	1983	6686716
stimulation of microperoxisomal beta-oxidation in rat heart by high-fat diets.	1. heart microperoxisomal beta-oxidation activity, measured as cyanide-insensitive palmitoyl-coa-dependent nad+-reduction, was detected in a microperoxisome-enriched fraction from rat myocardium. the effect on this microperoxisomal beta-oxidation of the fatty acid composition of the dietary oils was investigated. 2. feeding 15% (w/w) high erucic acid rapeseed oil or partially hydrogenated marine oil for 3 weeks increased the microperoxisomal beta-oxidation in the heart 4-5-fold, compared to a so ...	1983	6849945
the effects of partially hydrogenated marine oils on the mitochondrial function and membrane phospholipid fatty acids in rat heart.	the influence of dietary partially hydrogenated marine oils containing docosenoic acid on rat heart mitochondrial membrane phospholipid fatty acid composition was studied with particular reference to cardiolipin and oxidative phosphorylation. five groups of male weanling rats were fed diets containing 20% (w/w) peanut oil (po), partially hydrogenated peanut oil (hpo), partially hydrogenated norwegian capelin oil (hco), partially hydrogenated herring oil (hho), and rapeseed oil (rso) for 10 weeks ...	1983	6855477
the effect of dietary partially hydrogenated marine oils on desaturation of fatty acids in rat liver microsomes.	the influence of dietary partially hydrogenated marine oils on distribution of phospholipid fatty acids in rat liver microsomes was studied with particular reference to the metabolism of linoleic acid. five groups of weanling rats were fed diets containing 20% (w/w) peanut oil (po), partially hydrogenated peanut oil (hpo), partially hydrogenated norwegian capelin oil (hco), partially hydrogenated herring oil (hho), and rapeseed oil (rso) for 10 weeks. the partially hydrogenated oils were supplem ...	1983	6855478
induction of a reversible cardiac lipidosis by a dietary long-chain fatty acid (erucic acid). relationship to lipid accumulation in border zones of myocardial infarcts.	previous studies have demonstrated that cardiac myocytes in the border zone of acute myocardial infarction become markedly overloaded with neutral lipid during the transition from reversible to irreversible injury. to examine directly the role of these changes in neutral lipid metabolism in the development of irreversible cellular injury and associated increases in tissue ca2+ content, the authors fed rats large amounts of a fatty acid (erucic acid) that is poorly oxidized by the heart and that ...	1983	6859230
changes in the fatty acid patterns of brain phospholipids during development of rats fed peanut or rapeseed oil, taking into account differences between milk and maternal food.	the nature and amount of essential fatty acids in dietary fat play a leading part in the repartition of brain polyunsaturated fatty acids (n-3 and n-6). in order to determine precisely the respective roles of linolenic and linoleic acids in the diet on rat brain development, we used two diets in which the percentage of linolenic acid (18:3 n-3) was different. the animals were fed peanut oil (group a) or rapeseed oil (group b) during pregnancy and throughout lactation. the study of the fatty acid ...	1983	6859808
influence of dietary content of lipids and lipotropic nutrients on chemical carcinogenesis in rats.	high incidences of breast and colon tumors are associated with high dietary fat intake. the importance of specific fat type and of other nutrients that interact with fat, as well as the mechanisms by which fat acts, is being investigated in animal models. the amount and type of dietary fat influence mammary gland carcinogenesis in rats. tumor incidence and number are increased and latency is decreased in rats fed large amounts of corn oil, other polyunsaturated vegetable oils, or lard. certain f ...	1983	6187451
lipase in lipid bodies of cotyledons of rape and mustard seedlings.	lipolytic activity was absent in the crude cotyledon extract of ungerminated rapeseed (brassica napus l. var. dwarf essex), and increased to a peak at day 4 in seedling growth, concomitant with the decrease in total lipids. about 50% of the lipase activity was recovered in the lipid bodies isolated from the cotyledon extract by flotation centrifugation. isolated lipid bodies underwent autolysis of internal triacylglycerols resulting in the release of fatty acids. after the triacylglycerols in is ...	1983	6614927
uptake of 2,4-d in higher plants from artificial rain.	sinapis alba l., lapsana communis l., achillea millefolium l., brassica napus l., lactuca sativa l., and lycopersicum esculentum l. were exposed to 2,4-dichlorophenoxy [2-14c]acetic acid (2,4-d) at 10 micrograms liter-1 in artificial rain, ph 6.5 and 3.3. the 2,4-d was absorbed in all species tested. concentrations of parent 2,4-d appeared at the highest level in achillea (0.1 mg kg-1 dry wt), and at zero level in lycopersicum. twenty-one daily treatments at ph 6.5 for 30-min periods increased d ...	1984	6428858
a study of the effect of alpha-linolenic acid on the desaturation of dihomo-gamma-linolenic acid using rat liver homogenates.	the effects of alpha-linolenic acid on delta 5 desaturation of 20: 3 n-6 dihomo-gamma-linolenic acid) to 20: 4 n-6 (arachidonic acid) and on delta 6 desaturation of 18: 2 n-6 (linoleic acid) to 18:3 n-6 (gamma-linolenic acid) were investigated in two different studies using rat liver homogenates. in the first study, rats were fed a semi-synthetic fat-free diet. when unlabelled 18:3 n-3 was added to the incubation of 14c 18:2 or 14c 20:3, the desaturation rates decreased. delta 6 desaturation was ...	1984	6324307
some aspects of the composition of avian ascitic fluid.	ascitic fluid from male single comb white leghorn chickens fed a diet containing 20% by weight rapeseed oil rich (51.6 weight percent) in erucic (22:1n9) acid contained 2.84 +/- .09 g/dl protein and 2.55 +/- .22 micrograms/ml dna. a characteristic feature of ascitic fluid as compared to liver and plasma was its high polyunsaturates, low saturates, and low saturate to unsaturate ratio. in general, the fatty acid profile of ascitic fluid is more comparable to the fatty acid profile of plasma lipid ...	1984	6473250
effects of high erucic acid diet on sphingomyelin biosynthesis in rat lung microsomes.	the amount of fatty acids in free ceramides and sphingomyelins and the biosynthesis of sphingomyelins were measured in lung microsomes of rats fed either a stock diet or semisynthetic diets containing 15% by weight of sunflower oil or rapeseed oil (48% erucic acid). the rapeseed oil diet increased the amount of 24 : 1 in free ceramides and decreased that of 24 : 0 in both fractions as compared to the other reference diets. incubations of lyophilized microsomes with [14c acyl] ceramides (16 : 0, ...	1984	6527283
succinate dehydrogenase activity in relation with cardiac morphology in rats fed low erucic acid rapeseed oil.	the histopathological status and histologically demonstratable succinate dehydrogenase activity were evaluated on contiguous heart sections of rats fed low erucic acid rapeseed oil for 18 weeks. the histologically demonstratable sdh activity was quantified and could be related with the severity of the lesion at the same location. these results were discussed in terms of effects of dietary fat on mitochondria.	1984	6537736
changes in the content of n-6 fatty acids in liver phospholipids in rats as a consequence of partially hydrogenated dietary oils.	the effects of unhydrogenated and partially-hydrogenated marine and rapeseed oils on the relative content of n-6 fatty acids in rat liver phospholipids were studied by gas-liquid chromatography. the type of dietary oil strongly affected the pattern of n-6 fatty acids in the liver phospholipids even after a relatively short feeding period (3 weeks). with diets deficient in linoleic acid, accumulation of the 20:3 n-9 fatty acid was observed in the animals receiving partially hydrogenated oils, but ...	1984	6539124
modifications to swede (brassica napus l.) anterior to the terminal ileum of pigs: some implications for the analysis of dietary fibre.	the degradation of a swede (brassica napus l., cv. danestone) diet anterior to the terminal ileum was studied in two pigs fitted with t-shaped cannulas 150 mm before the ileo-caecal junction. digestibility was calculated with reference to chromic oxide and polyethylene glycol. in addition to the total loss of free glucose and fructose, there were substantial modifications to cell-wall material recovered in digesta. these included the apparent loss of 46-50% of uronic acid residues, 72-78% of phe ...	1984	6093857
long-chain acyl-coa levels in liver from rats fed high-fat diets: is it of significance for an increased peroxisomal beta-oxidation?	the levels of long-chain acyl-coa in the livers of rats given diets containing various amounts of dietary oils were investigated. increasing the amount of soybean oil in the diet from 5% to 25% (w/w) led to a 40% increase in long-chain acyl-coa. with partially hydrogenated marine oil, a sigmoidal dose-response curve was obtained, giving a 60% increase when 20% or more of this oil was in the diet. all high-fat diets tested resulted in higher levels of long-chain acyl-coa than the low-fat control ...	1984	6717250
influence of dietary rapeseed oil and erucic acid upon myocardial performance and hemodynamics in rats.	rats fed rapeseed oil, pure erucic acid, or a control diet of sunflowerseed oil during 24-26 weeks were studied for effects upon mechanical behavior of the isolated perfused heart and upon myocardial performance and hemodynamics in intact animals both under basal and stimulated conditions. in spite of focal myocardial fibrotic lesions due to rapeseed oil, no changes were found with respect to the intrinsic myocardial contractility in vitro and and in vivo. after inotropic intervention, only the ...	1984	6729825
effect of ingestion of thermally oxidized frying oil on peroxidative criteria in rats.	thermally oxidized rapeseed oils (4 levels of deterioration; used by a manufacturer of fried fish paste in a conventional manner) were fed to rats at a practical level of concentration. rats were fed a diet ad libitum for 13 weeks that contained 15% of a test oil. the effects of the diet on several biochemical criteria related to peroxidative alterations were investigated. in groups given thermally oxidized oils relative liver weight, relative kidney weight, thiobarbituric acid-reactive substanc ...	1984	6738310
evolution of the alimentary toxic oil syndrome due to ingestion of denatured rapeseed oil.	three hundred seventeen patients with toxic oil syndrome caused by ingestion of denatured rapeseed oil were followed up after one year. total mortality for the 317 patients was 4.4%. after 12 months, 3.2% of the surviving patients have a severe motor neuropathy, 52.9% are asymptomatic, and the remaining 39.4% have mild residual myalgia. the cause of death in seven patients during the acute phase of toxic oil syndrome was respiratory failure due to interstitial pneumonitis with cerebral edema. th ...	1984	6696559
pulmonary hypertension after toxic rapeseed oil ingestion.		1984	6549605
high frequency of fusion induced in freely suspended protoplast mixtures by polyethylene glycol and dimethylsulfoxide at high ph.	fusion of freely suspended protoplast mixtures (hypocotyl protoplasts of brassica napus mixed with mesophyll protoplasts of either b. campestris or nicotiana plumbaginifolia) was induced by a solution containing 10% polyethylene glycol, 10% dimethyl-sulfoxide and 0.1m glycine-naoh buffer (ph 10.0). the fusion products represented 15 to 17 percent of the surviving cells. more than 50% of the fusion products divided within two days after fusion, indicating that the fusion procedure did not signifi ...	1984	24253515
precociously germinating rapeseed embryos retain characteristics of embryogeny.	we compared the germination of brassica napus l. embryos at three stages of development-mid-cotyledon, maturation and mature dry-to determine at which stage they acquired the capacity for normal germination and seedling development. embryos were removed from the seed and cultured on hormone-free medium, allowing them to germinate. the transition from embryogeny to germination was monitored both morphologically and biochemically, using synthesis of 12 s storage protein as a marker of embryogeny. ...	1984	24254046
control of seed germination by abscisic acid : ii. effect on embryo water uptake in brassica napus l.	germination of rape (brassica napus l.) seeds proceeds in two phases, an initial imbibition phase and a subsequent growth phase. the time courses of water uptake, o(2) uptake, and atp accumulation demonstrate that exogenous abscisic acid (aba, 0.1 millimoles per liter) specifically prevents the embryo from entering the growth phase. the inhibition of water uptake by aba is a rapid (lag-phase about 1 hour) and fully reversible process which appears to be the cause rather than the result of change ...	1984	16663788
effects of 2,4-dichlorophenoxyacetic acid in artificial acid rain on brassica napus and sinapis alba.	the effect of 2,4-dichlorophenoxyacetic (2,4-d) acid in artificial rain at ph 3.3 was examined for young plants of rape (brassica napus l.) and white mustard (sinapis alba l.). plants were treated in a closed simulation chamber system with varying co2 supply (198-418 ppm). single or daily showers were applied for 20-30 min at varying 2,4-d concentrations (0, 0.03, 0.1, 0.5, and 2.5 mg liter-1). the plants were sampled 5 days after the last treatment. epinasty was observed for brassica at 0.5 mg ...	1984	6479080
cortical microtubular lattices: absent from mature mesophyll and necessary for cell division?	using immunofluorescence microscopy, the cortical microtubular net which is regularly present in cells of young, growing tissue is shown to be absent, or largely reduced, in mature mesophyll cells of nicotiana plumbaginifolia viv., n. tabacum l., petunia hybrida hort. and brassica napus l. the onset of division in protoplasts isolated from these fully differentiated tissues is preceded by a period of dedifferentiation. one of the early events during dedifferentiation, as shown for n. plumbaginif ...	1985	24241511
an evaluation of the role of ethylene in herbicidal injury induced by picloram or clopyralid in rapeseed and sunflower plants.	the role of ethylene in herbicidal injury induced by 4-amino-3,5,6-trichloropicolinic acid (picloram) or 3,6-dichloropicolinic acid (clopyralid) was investigated in sunflower (helianthus annuus l.) and rapeseed (brassica napus l. cv altex). picloram induces herbicide injury in both species, whereas clopyralid induces injury only in sunflower. picloram applied to the third leaf of a rapeseed plant increased ethylene evolution several-fold. clopyralid had no effect on ethylene production in rapese ...	1985	16664366
fusion characteristics of plant protoplasts in electric fields.	the electrical parameters important in the fusion of plant protoplasts aligned dielectrophoretically in high-frequency alternating electric fields have been established. protoplasts were aligned in an alternating electric field between two relatively distant (1 mm) electrodes, by dielectrophoresis induced by field inhomogeneities caused by the protoplasts themselves. this arrangement allowed ease of manipulations, large throughput and low loss of protoplasts. in analytical experiments, sufficien ...	1985	24241045
role of aba in maturation of rapeseed embryos.	development of brassica napus l. cv tower embryos of different ages cultured in vitro with and without abscisic acid (aba) was compared with normal development in situ to investigate the role of aba in embryo maturation. endogenous aba levels were measured by radioimmunoassay, and sensitivity to aba was assayed in terms of its ability to suppress precocious germination and stimulate accumulation of storage protein and storage protein mrna. during development in situ, the levels of endogenous aba ...	1985	16664296
nucleotide sequence of a cdna clone of brassica napus 12s storage protein shows homology with legumin from pisum sativum.	the most abundant protein in seeds of brassica napus (l.) is cruciferin, a legumin-like 12s storage protein. by in vitro translation of embryo rna, and pulse-chase labelling of cultured embryos with (14)c-leucine, we have shown that the 30 kd α polypeptides and 20 kd β polypeptides of cruciferin are synthesized as a family of 50 kd precursors which are cleaved post-translationally. one member of the cruciferin family was cloned from embryo cdna and sequenced. the nucleotide sequence of the cruci ...	1985	24306655
semi-synthetic preparation of 1-o-[1'-14c]hexadecyl-2-acetyl-sn-glycero-3-phosphocholine (platelet activating factor) using plant cell cultures.	incubation of photomixotrophic cell suspension cultures of rape (brassica napus) and heterotrophic cell suspension cultures of soya (glycine max) with 1-o-[1'-14c]hexadecyl-sn-glycerol or rac-1-o-[1'-14c]hexadecylglycerol leads in high yield (up to 78%) to labeled 1-o-hexadecyl-2-acyl-sn-glycero-3-phosphocholines. alkaline hydrolysis of the choline glycerophospholipids yields pure 1-o-[1'-14c]hexadecyl-sn-glycero-3-phosphocholine. 1-o-[1'-14c]hexadecyl-2-acetyl-sn-glycero-3-phosphocholine (plate ...	1985	3891896
control of seed germination by abscisic acid : iii. effect on embryo growth potential (minimum turgor pressure) and growth coefficient (cell wall extensibility) in brassica napus l.	the physical mechanism of seed germination and its inhibition by abscisic acid (aba) in brassica napus l. was investigated, using volumetric growth (= water uptake) rate (dv/dt), water conductance (l), cell wall extensibility coefficient (m), osmotic pressure ( product operator(i)), water potential (psi(i)), turgor pressure (p), and minimum turgor for cell expansion (y) of the intact embryo as experimental parameters. dv/dt, product operator(i), and psi(i) were measured directly, while m, p, and ...	1985	16664118
a simple culture method for brassica hypototyl protoplasts.	hypocotyl protoplasts from oil rape, brassica napus l. cv. isuzu were cultured in the dark at 25°c in a modified nitsch and nitsch medium containing 13% sucrose, 5 g/l ficoll, 0.5 mg/l bap, 1 mg/l naa and 0.5 mg/l 2-4 d. protoplasts floated on the surface of the medium and developed into microcolonies 0.5 mm in diameter in 4-6 weeks. the microcolonies also remained on the surface of the medium. transfer to ms medium supplemented with 200 mg/l casein hydrolysate, 5mg/l bap, 0.5 mg/l naa and solid ...	1985	24253633
ether glycerolipids: novel substrates for studying specificity of enzymes involved in glycerolipid biosynthesis in higher plants.	ether glycerolipids, predominantly alkylacylglycerols and alkylacylglycerophosphocholines, are synthesized in photomixotrophic rape (brassica napus) suspension cells from various exogenous monoalkylglycerols. the stereospecific distribution of acyl moieties was studied in these ether glycerolipids with regard to chain-length and degree of unsaturation of alkyl moieties and compared with the distribution of acyl moieties in the corresponding endogenous acyl glycerolipids. the results show the fol ...	1985	3967662
initiation of postmeiotic beta-galactosidase synthesis during microsporogenesis in oilseed rape.	the synthesis of beta-galactosidase during brassica campestris pollen development results from the transcription of the haploid genome. a quantitative cytochemical method has been developed in which 5-bromo-4-chloro-3-indoxyl-beta-d-galactoside is used as substrate giving a blue-green final reaction product. we have recently detected oilseed rape plants which are heterozygous for the beta-galactosidase locus, in which 50% of the pollen grains produced are gal (having enzyme activity), while the ...	1985	16664014
purification, properties and amino acid sequence of a low-mr abundant seed protein from pea (pisum sativum l.).	the seeds of pea (pisum sativum l.) contain several proteins in the albumin solubility fraction that are significant components of total cotyledonary protein (5-10%) and are accumulated in developing seeds concurrently with storage-protein synthesis. one of these proteins, of low mr and designated 'psa la', has been purified, characterized and sequenced. psa la has an mr of 11000 and contains polypeptides of mr 6000, suggesting that the protein molecules are dimeric. the amino acid sequence cont ...	1985	3977826
glycosidase activities in sera from convalescent patients who ingested a toxic oil.	some glycosidase activities have been determined in blood sera from 21 patients who ingested a toxic oil (rapeseed oil denatured with aniline(s) and treated by a thermal process). the samples were collected from the same patients on 3 or 4 occasions during a period of 11-12 mth. during this period, the clinical state of the patients improved and, generally, they were restored to health. beta-n-acetylglucosaminidase, alpha-l-fucosidase, beta-d-glucuronidase, beta-d-glucosidase and alpha-d-mannosi ...	1985	3987046
modified determination of 2-thiobarbituric acid value in fats and oils.	several methods for the determination of tba-value were compared with a new direct modification (omitting the previous distillation), using 1-butanol as the sole solvent. the absorbance is measured at 530 nm after heating to 95 degrees c for 120 min, and cooling. the effect of variables was tested by the youdens ruggedness test (reaction time, temperature, cooling, concentration of sample, thiobarbituric acid, water, heavy metals, antioxidants, chelating agents, bubbling with oxygen or nitrogen) ...	1985	3990777
mitral valve prolapse secondary to right ventricular enlargement in patients with pulmonary hypertension after toxic rapeseed oil ingestion.	a high incidence of mitral valve prolapse (mvp) has been reported in various entities which produce important right ventricular (rv) enlargement with normal or decreased left ventricular (lv) volume. to evaluate the importance of rv enlargement in the genesis of mvp in these cases, we analyzed the echocardiographic studies from 176 patients with 'síndrome tóxico'. these patients underwent m-mode, cross-sectional and pulsed doppler examination because of the suspicion of having dietary pulmonary ...	1985	3159573
evaluation of the sweep co-distillation cleanup technique for the determination of environmental contaminants in human adipose tissue.	beef and pork fat, corn, peanut, rapeseed and paraffin oils, as well as vegetable shortening were used to investigate their suitability as fortification media for environmental chemicals in the evaluation of the sweep co-distillation technique. the animal fats produced considerable gas chromatographic background interference, while the oils were partly carried over during the sweep co-distillation process, except for rapeseed and peanut oil. residue free rapeseed oil was fortified with 26 enviro ...	1985	3980137
[specific contribution of dietary polyunsaturated fatty acids of the n-3 series to the development of nervous system membranes].	polyunsaturated fatty acids, especially omega 3 fatty acids, made a specific contribution to the nervous tissue. retarded intrauterine growth caused a twofold reduction in the oligodendrocyte ratio of omega 3/omega 6 while the same ratio increased by more than twofold in the neurons. a fatty acid-poor diet composed of arachid or sunflower oil dramatically altered the composition of cells and subcellular particles when compared with a soya or rapeseed oil diet containing omega 3 fatty acids; phos ...	1985	3992003
influence of dietary partially hydrogenated vegetable and marine oils on membrane composition and function of liver microsomes and platelets in the rat.	the aim of the present study was to investigate the influence of partially hydrogenated vegetable and marine oils on membrane composition and function of liver microsomes and platelets with particular reference to the metabolism of linoleic acid and the production of arachidonic acid metabolites. four groups of male weanling rats were fed linoleic acid supplemented diets containing 20% (w/w) of partially hydrogenated low erucic acid rapeseed oil (hlrso), partially hydrogenated herring oil (hho), ...	1985	4010485
pathological changes associated with the feeding of soybean oil or oil extracted from different rapeseed cultivars to single comb white leghorn cockerels.	a total of 384 single comb white leghorn cockerels of the hyline strain were fed either a basal (control) diet containing no added oil or a diet supplemented with 20% by weight of soybean oil, rapeseed oil from cultivars tower, candle, a mixture of echo and arlo high erucic acid rapeseed (hear), or r-500. levels of erucic acid (22:1) in the rapeseed diets varied from .03 to 10.31%. three birds from each unit were killed at 28, 56, 84, and 112 days and a wide range of tissues were examined histol ...	1985	4048047
cardiac lipid changes in rats fed oils enriched in saturates and their apparent relationship to focal heart lesions.	weanling male sprague-dawley rats were fed diets containing 20% by weight corn, soybean or low erucic acid rapeseed oils or mixtures of the latter two with cocoa butter or triolein for 1, 2, 3 or 4 weeks. these diets previously had been fed to the same strain of rats for 16 weeks, and a reduction in the incidence of focal heart lesions had been observed with the addition of cocoa butter, but not triolein. the cardiac lipid classes and the fatty acid and alkenyl ethers of the cardiac phospholipid ...	1985	4058262
estimation of microbial nitrogen in nylon-bag residues by feed 15n dilution.	rapeseed (brassica napus), barley grain, ryegrass (lolium perenne) and barley straw were labelled with 15n as an internal marker. the dilution of 15n was used to estimate microbial n (rmn15n) in the feed residues in nylon bags incubated in the rumen for 5, 12 and 24 h. for comparative purposes, diaminopimelic acid (dap) content of the residues was also determined and rumen microbial n (rmndap-n) in the feed residues calculated using dap as a bacterial marker. the influence of two bag pore sizes ...	1985	4063332
effect of dietary erucic acid on the utilization of oils or fats by growing chicks.	free erucic acid (ea) (c22:1) was blended with cambra oil (cbo), sunflower oil (sfo), and animal lard (al) at 45% (w/w). six experimental diets were formulated to incorporate either cbo, sfo, al, or ea blended oils (cbo plus ea, sfo plus ea), or fats (al plus ea) at 8%. the adverse effects of dietary ea blended with various types of oils or fats were studied by feeding 180-day-old white leghorn cockerels for a 4-week growing period. in general, adverse effects of dietary ea were clearly reflecte ...	1985	4070144
[exogenous lipid pneumonia after occupational rapeseed oil inhalation].	a 26 year-old man developed a lipid pneumonia with an intensely granulomatous inflammation following repeated occupational exposure to aerosolized rape seed oil. the intensity of the reaction, greater than that customarily seen with vegetable oil induced pneumonias, was attributed to specific components of rape seed oil.	1985	4096137
effects of high fat diets on the activity of palmitoyl-coa hydrolase in rat liver.	palmitoyl-coa hydrolase [ec 3.1.2.2.] activity in rat liver was found to be enhanced by high fat diets. partially hydrogenated marine oil and high-erucic acid rapeseed oil diets produced a greater increase than a diet containing soybean oil. with diets containing from 5 to 30% (w/w) of partially hydrogenated marine oil the increase in palmitoyl-coa hydrolase activity was similar to the increase observed in peroxisomal beta-oxidation activity (correlation coefficient r = 0.94). a positive correla ...	1985	2857471
genetic manipulation in cultivars of oilseed rape (brassica napus) using agrobacterium.	the response of oilseed rape cultivars to infection with agrobacterium tumefaciens and a. rhizogenes and the possibility of regenerating genetically transformed oilseed rape plants were examined. the frequency at which agrobacterium induced galls or hairy-roots on in vitro cultured plants ranged from 10% to 70%, depending on the cultivar. from galls induced by the tumorigenic strain t37, known to be strongly shoot inducing on tobacco, roots developed frequently. occasionally, shoots formed and s ...	1985	24247402
analysis of storage proteins in normal and aborted seeds from embryo-lethal mutants of arabidopsis thaliana.	the major storage proteins isolated from wild-type seeds of arabidopsis thaliana (l.) heynh., strain "columbia", were studied by sucrose gradient centrifugation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). both the hypocotyl and cotyledons of mature embryos contained abundant 12 s (cruciferin) and 2 s (arabin) proteins that appeared similar in size and subunit composition to the cruciferin (12 s) and napin (1.7 s) seed-storage proteins of brassica napus. the 12 s pro ...	1986	24232640
the amino-acid sequence of the 2s sulphur-rich proteins from seeds of brazil nut (bertholletia excelsa h.b.k.).	storage proteins of the albumin solubility fraction from seeds of bertholletia excelsa h.b.k. were separated by reversed-phase high-performance liquid chromatography and their primary structures were determined by gas-phase sequencing on intact polypeptides and on the overlapping tryptic and thermolysin peptides. the 2s storage proteins consist of two subunits linked by disulphide bridges. the large subunit (8.5 kda) is expressed in at least six different isoforms while the small subunit (3.6 kd ...	1986	3758080
neurotransmitter changes in cerebrospinal fluid in the spanish toxic oil syndrome: human clinical findings and experimental results in mice.	patients with spanish toxic oil syndrome (tos) complaining of neuromuscular symptoms had increased levels of homovanillic acid and 5-hydroxyindoleacetic acid (5-hiaa) in cerebrospinal fluid. severity of pain and muscle cramps correlated with the magnitude of increment in levels of monoamine metabolites. mice treated with oleyl anilide, a putative toxic compound found in some stocks of the toxic rapeseed oil, did not present clinical or anatomical findings compatible with tos. however, biochemica ...	1986	2425298
comparison of the effect of cell wall and hull fiber from canola and soybean on the bioavailability for rats of minerals, protein and lipid.	hull or cell wall material, isolated from canola (brassica napus cv. regent) or soybean (glycine max) or cellulose was added to a basal, semipurified diet at a level of 12% and fed to growing male wistar rats. the apparent availability of cu, fe, ca, p and protein were lower when the fiber-containing diets were fed compared to the control diet. the availability of mg was lower when the canola hull (ch) and cellulose (ce) diets were fed, whereas zn availability was lower when the canola cell wall ...	1986	3003294
inhibition of glucose-6-phosphatase and adenosine-triphosphatase activity in rats treated with toxic spanish rapeseed oil or synthetic anilides.	after having demonstrated the impairment of the microsomal oxidation process in rats treated with toxic spanish cooking oil or fatty acid anilides, we studied the possibility that the function of the cytoplasmic membrane had been affected. for this, the activities of glucose-6-phosphatase and adenosintriphosphatase were determined, these being enzymes of utmost importance in transport at the liver cell membrane level. wistar rats received disease-related toxic rapeseed oil (1 mg/kg/day) or linol ...	1986	3022461
microbially corrected amino acid composition of rumen-undegraded feed protein and amino acid degradability in the rumen of feeds enclosed in nylon bags.	1. in the previous work (varvikko & lindberg, 1985), 15n-labelled rapeseed (brassica napus), barley, ryegrass (lolium perenne) and barley straw were incubated in the rumen in nylon bags for 5, 12 and 24 h and microbial nitrogen in the residues was quantified using the feed 15n-dilution method. in the present study, residual amino acids (aa) of these feeds were analysed, and microbially corrected aa of feed origin (feed aa) were estimated as the difference between total residual aa and respective ...	1986	3676189
the effect of high-fat diets on microsomal lauric acid hydroxylation in rat liver.	a diet with 20% (w/w) fish oil or partially hydrogenated fish oil has been shown to stimulate omega-oxidation of lauric acid 2.5-fold with rat liver microsomal preparations after 1 week of feeding. a diet containing either 20% (w/w) soybean oil, partially hydrogenated soybean oil or rapeseed oil had no effect. the omega-oxidation was also stimulated by fasting (3.7-fold) and by clofibrate (13-fold). the stimulation of omega-oxidation with partially hydrogenated fish oil was at its highest level ...	1986	3768400
quantitative determination of triacylglycerols separated on capillary columns according to acyl carbon number and level of unsaturation.	the quantitative determination of triacylglycerols separated according to acyl carbon numbers and level of unsaturation was studied on two 10-m immobilised se-54 columns. the fat samples were silylated before on-column injection. the 0.2 mm i.d. column showed higher resolving power and the 0.32 mm i.d. column better repeatability. the application to real quantitations was investigated with analyses of butter-fat solid and liquid fractions and of untreated and interesterified 70-30 mixtures of bu ...	1986	3782362
influence of the fatty acid composition of high-density lipoprotein phospholipids on the cholesterol efflux from cultured fibroblasts.	the purpose of this work was to determine whether the changes induced by dietary manipulations in the chemical composition of high-density lipoproteins (hdl) (particularly phospholipid fatty acid composition) modified their capacity to promote [3h]cholesterol efflux from cultured fibroblasts. plasma hdl were obtained from subjects fed for six successive long periods on diets consisting of one predominant fat: peanut oil, corn oil, olive oil, soybean oil, low erucic acid rapeseed oil or milk fats ...	1986	3942761
seasonal population changes and characterization of ice-nucleating bacteria in farm fields of central alberta.	during the summer of 1983 in central alberta, changes in the bacterial population inhabiting the leaves of field beans (phaseolus vulgaris l.) and canola (brassica napus l. altex) were studied to determine if ice-nucleating bacteria were present on these plants. three colony types (white, yellow, and peach-colored) were found on field beans and canola leaves. approximately 25% of the isolates from the white colony group, which dominated the population, were ice-nucleating bacteria. no ice-nuclea ...	1986	16347106
ultrastructural study of blood cells in toxic oil syndrome.	the toxic oil syndrome is a new multisystemic disease, linked to the consumption of denatured rapeseed oil, which occurred in spain in 1981. the hematological symptoms, present in about all the patients since the beginning of this disease have been dominated by leukocytosis and eosinophilia. the ultrastructural study of circulating blood cells has shown the presence of multiple abnormal lipid vacuoles in the eosinophil series and less frequently in neutrophils, monocytes and lymphocytes, associa ...	1986	3092533
the introduction of cms mitochondria to triazine tolerant brassica napus l., var. "regent", by micromanipulation of individual heterokaryons.	cytoplasmic triazine tolerance and cytoplasmic male sterility traits were combined in the nuclear genomic background of the brassica napus variety 'regent', following protoplast fusion, selection of fusion products by manual micro-manipulation, and culture in a nicotiana tabacum nurse system. whole plant cybrid regenerants were morphologically normal and produced seed on pollination, demonstrating their potential for incorporation into a breeding program.	1986	24248394
structure of the rapeseed 1.7 s storage protein, napin, and its precursor.	napin (1.7 s protein) is a basic, low molecular weight storage protein synthesized in rapeseed (brassica napus) embryos during seed development. napin is composed of two polypeptide chains with molecular weights of 9000 and 4000 that are held together by disulfide bonds. comparison of the deduced amino acid sequence of a napin cdna clone with that of napin peptide fragments established that napin is initially synthesized as a precursor of 178 residues. this polypeptide is subsequently processed ...	1986	3771543
characterization of triazine-resistant and -susceptible isolines of canola (brassica napus l.).	morphometric, electrophoretic, and immunological procedures were used to probe the structural and physiological differences between triazine-resistant (r) and susceptible (s) isolines of canola (brassica napus l.). the r biotype exhibited increased grana stacking and decreased amounts of starch compared to the s biotype. likewise, characters associated with an increase in grana stacking (lower chlorophyll a/b ratio, increased chlorophyll a/b light-harvesting complex, and relatively lower amounts ...	1986	16665122
changes in protein synthesis in rapeseed (brassica napus) seedlings during a low temperature treatment.	changes induced by cold treatment in young rapeseed (brassica napus) seedlings were investigated at the molecular level. following germination at 18 degrees c for 48 hours, one half of the seedlings was transferred to 0 degrees c for another 48 hour period, the other half being kept at 18 degrees c as a control. newly synthesized proteins were labeled for the last 6 hours of incubation with [(35)s]methionine. the different polypeptides were separated by two-dimensional electrophoresis in polyacr ...	1986	16665102
inheritance of s-methyl-l-cysteine sulphoxide and thiocyanate contents in forage rape (brassica napus l.).	inheritance of thiocyanate and (+)s-methyl-l-cysteine sulphoxide (smco) contents were studied in a diallel cross involving five varieties of forage rape. although both additive and dominance components were significant for the two characters, the greater mean squares of the additive gene effect indicated its greater importance. non-allelic gene interaction was detected for thiocyanate only. narrow sense heritability was relatively high for both characters. the general and specific combining abil ...	1986	24248085
rapeseed embryo development in culture on high osmoticum is similar to that in seeds.	the development of brassica napus l. cv tower embryos of different ages cultured in vitro with and without high osmoticum (0.48 and 0.69 molar sorbitol) was compared with normal development in situ to investigate the role of a drying environment in embryo maturation. sensitivity to osmoticum was assayed in terms of its ability to mimic normal development, i.e. to both suppress germination and maintain 12 s storage protein (cruciferin) synthesis at levels comparable to those seen in the developin ...	1986	16664923
model system for studies of microbial dynamics at exuding surfaces such as the rhizosphere.	an autoclavable all-glass system for studying microbial dynamics at permeable surfaces is described. standard hydrophobic or hydrophilic membranes (46-mm diameter) of various pore sizes were supported on a glass frit through which nutrient solutions were pumped by a peristaltic pump. the pump provided a precisely controlled flow at speeds of 0.5 to 500 ml of defined or natural cell exudates per h, which passed through the membrane into a receiving vessel. the construction allowed a choice of mem ...	1986	11536565
synchronization of protoplasts from glycine max (l.) merr. and brassica napus (l.).	cells of glycine max originating in a suspension culture and cells of brassica napus prepared from hypocotyls were synchronized. synchronization was achieved by preparing protoplasts in the usual way and subsequently letting the protoplasts regenerate into cells by removing the cell-wall-digesting enzymes. more than 70% of the cells had divided synchronously at the end of the first cycle as determined by the mitotic index. the high frequency of mitosis critically depended on the osmolality of th ...	1986	24232032
oilseed rape nadh enoyl acyl-carrier protein reductase.		1986	3732599
a rapid and efficient alternative procedure for the regeneration of plants from hypocotyl protoplasts of brassica napus.	protoplasts of several spring and winter varieties of brassica napus were isolated from hypocotyl tissue. protoplasts divided and formed cell colonies at high frequency, without browning when cultured in modified shepards' medium. this high efficiency of proliferation was sustained through to plant regeneration with all varieties cultured. this has been attributed to the incorporation of a reservoir medium, the presence of 2,4-d in the proliferation medium, and the presence of kinetin in conjunc ...	1986	24248044
cytoplasmic male sterility in rapeseed (brassica napus l.) : 2. the role of a mitochondrial plasmid.	the linear mitochondrial (mt) plasmid in rapeseed occurs in normal, fertile plants, cytoplasmic male sterile (cms) lines (sterile and restored) and maintainers. to determine the role of plasmid genes in cytoplasmic male sterility the three internal hind iii fragments (78%) of the 11.3 kb plasmid were cloned, nick-translated and hybridized to electrophoretic blots of pst i digests of chloroplast (cp) and mt dna from normal and cms lines. there is no homology between plasmid dna and the cp and mt ...	1986	24247828
cytoplasmic male sterility in rapeseed (brassica napus l.) : 1. restriction patterns of chloroplast and mitochondrial dna.	restriction patterns of chloroplast (cp) and mitochondrial (mt) dna in brassica napus rapeseed reveal the alloplasmic nature of cytoplasmic male sterility in this crop. both the shiga and bronowski systems probably exploit cytoplasmic diversity in b. napus cultivars arising from introgression of cytoplasm from the other rapeseed species, b. campestris. nuclear genes specific to these systems do not cause sterility in maintainers (bronowski and isuzu-natane) because they have a campestris cytopla ...	1986	24247827
mapping the chloroplast genome of triazine resistant canola.	the chloroplast of a triazine resistant weed biotype of brassica campestris (bird's rape) has been transferred by repeated back-crossing into an agriculturally important strain, 'tower', of brassica napus to form a triazine resistant cultivar of canola, 'triton', that is low in both erucic acid and thioglucosinolate. in this report, the b. campestris derived chloroplast chromosome of b. napus (cv 'triton') has been cloned into bacterial plasmids and physically mapped for eight restriction enzyme ...	1986	24247607
stability of chloroplastic triazine resistance in rutabaga backcross generations.	triazine resistance originally observed in a weed biotype of birdsrape (brassica campestris l.) has been transferred through cytoplasmic substitution into rutabaga (brassica napus ssp. rapifera [metzg.] minsk.) by conventional backcrossing. photosynthetic function and resistance to triazines were examined in six backcross generations of rutabaga as well as in the original parents. chloroplast thylakoid membranes were isolated and their sensitivity to atrazine, metribuzin, and diuron assayed by m ...	1986	16664653
nucleotide sequence of a member of the napin storage protein family from brassica napus.	we have begun the molecular characterization of genes encoding napin, the 1.7 s embryo-specific storage protein of brassica napus. genomic southern blot analysis indicates that napin is encoded by a multigene family comprised of a minimum of 16 genes. two dna fragments containing single napin genes have been recovered from b. napus genomic libraries. we have determined the nucleotide sequence of one member of the napin gene family, gna. the gene has a simple structure lacking introns and contain ...	1987	3040733
drought-induced changes in protein patterns of brassica napus var. oleifera roots.	drought-induced changes in two-dimensional silver stained protein patterns of brassica napus l. var. oleifera m. root system were detected both at quantitative and qualitative levels. particularly, 13 new polypeptides of low molecular weight were evidenced in the drought-stressed tap root, 12 of which were also present in the short tuberized roots, a specific drought-induced root type. the reversibility of these modifications, observed after 3 days rehydration, suggests that they might be involv ...	1987	16665634