Publications

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selenium accumulating leafy vegetables are a potential source of functional foods.selenium deficiency in humans has been associated with various diseases, the risks of which can be reduced through dietary supplementation. selenium accumulating plants may provide a beneficial nutrient for avoiding such illnesses. thus, leafy vegetables such as amaranthus hybridus, amaranthus sp., cucurbita maxima, ipomoea batatas, solanum villosum, solanum scabrum, and vigna unguiculata were explored for their capabilities to accumulate selenium when grown on selenium enriched soil and for use ...201526955635
the making of giant pumpkins: how selective breeding changed the phloem of cucurbita maxima from source to sink.despite the success of breeding programmes focused on increasing fruit size, relatively little is known about the anatomical and physiological changes required to increase reproductive allocation. to address this gap in knowledge, we compared fruit/ovary anatomy, vascular structure and phloem transport of two varieties of giant pumpkins, and their smaller fruited progenitor under controlled environmental conditions. we also modelled carbon transport into the fruit of competitively grown plants u ...201525546629
high throughput sequencing of small rnas in the two cucurbita germplasm with different sodium accumulation patterns identifies novel micrornas involved in salt stress response.micrornas (mirnas), a class of small non-coding rnas, recognize their mrna targets based on perfect sequence complementarity. mirnas lead to broader changes in gene expression after plants are exposed to stress. high-throughput sequencing is an effective method to identify and profile small rna populations in non-model plants under salt stresses, significantly improving our knowledge regarding mirna functions in salt tolerance. cucurbits are sensitive to soil salinity, and the cucurbita genus is ...201526010449
characterization and pathogenicity of alternaria burnsii from seeds of cucurbita maxima (cucurbitaceae) in bangladesh.in the course of survey of endophytic fungi from bangladesh pumpkin seeds in 2011~2012, two strains (cnu111042 and cnu111043) with similar colony characteristics were isolated and characterized by their morphology and by molecular phylogenetic analysis of the internal transcribed spacer, glyceraldehydes-3-phosphate dehydrogenase (gpd), and alternaria allergen a1 (alt a1) sequences. phylogenetic analysis of all three sequences and their combined dataset revealed that the fungus formed a subclade ...201526839497
sequence variations in the fad2 gene in seeded pumpkins.seeded pumpkins are important economic crops; the seeds contain various unsaturated fatty acids, such as oleic acid and linoleic acid, which are crucial for human and animal nutrition. the fatty acid desaturase-2 (fad2) gene encodes delta-12 desaturase, which converts oleic acid to linoleic acid. however, little is known about sequence variations in fad2 in seeded pumpkins. twenty-seven fad2 clones from 27 accessions of cucurbita moschata, cucurbita maxima, cucurbita pepo, and cucurbita ficifoli ...201526782391
aphid salivary proteases are capable of degrading sieve-tube proteins.sieve tubes serve as transport conduits for photo-assimilates and other resources in angiosperms and are profitable targets for piercing-sucking insects such as aphids. sieve-tube sap also contains significant amounts of proteins with diverse functions, for example in signalling, metabolism, and defence. the identification of salivary proteases in acyrthosiphon pisum led to the hypothesis that aphids might be able to digest these proteins and by doing so suppress plant defence and access additio ...201525540441
cucumber (cucumis sativus l.) and kabocha squash (cucurbita moschata duch).we established improved methods for agrobacterium-mediated transformation of cucumber (cucumis sativus l.) and kabocha squash (cucurbita moschata duch). vacuum infiltration of cotyledonary explants with agrobacterium suspension enhanced the agrobacterium infection efficiency in the proximal regions of explants. wounding treatment was also essential for kabocha squash. cocultivation on filter paper wicks suppressed necrosis of explants, keeping regeneration efficacy. putative transgenic plants we ...201525300850
instrumental and sensory analyses of quality attributes of grafted specialty melons.soilborne disease management remains a great challenge in melon production with the phaseout of soil fumigant methyl bromide. grafting has been shown to be an effective approach to control soilborne diseases. however, previous research has yielded mixed results regarding the impacts of rootstock on fruit quality. very few studies have assessed melon quality attributes using both sensory evaluation and instrumental methods.201525512001
herbivore-triggered electrophysiological reactions: candidates for systemic signals in higher plants and the challenge of their identification.in stressed plants, electrophysiological reactions (elrs) are presumed to contribute to long-distance intercellular communication between distant plant parts. because of the focus on abiotic stress-induced elrs in recent decades, biotic stress-triggered elrs have been widely ignored. it is likely that the challenge to identify the particular elr types (action potential [ap], variation potential, and system potential [sp]) was responsible for this course of action. thus, this survey focused on in ...201626872949
proteomics and metabolomics analyses reveal the cucurbit sieve tube system as a complex metabolic space.the plant vascular system, and specifically the phloem, plays a pivotal role in allocation of fixed carbon to developing sink organs. although the processes involved in loading and unloading of sugars and amino acids are well characterized, little information is available regarding the nature of other metabolites in the sieve tube system (sts) at specific sites along the pathway. here, we elucidate spatial features of metabolite composition mapped with phloem enzymes along the cucurbit sts. phlo ...201627155400
electrophysiology of pumpkin seeds: memristors in vivo.leon chua, the discoverer of a memristor, theoretically predicted that voltage gated ion channels can be memristors. we recently found memristors in different plants such as the venus flytrap, mimosa pudica, aloe vera, apple fruits, and in potato tubers. there are no publications in literature about the existence of memristors in seeds. the goal of this work was to discover if pumpkin seeds might have memristors. we selected cucurbita pepo l., cv. cinderella, cucurbita maxima l. cv warty goblin, ...201626926652
effect of african leafy vegetables on the micronutrient status of mildly deficient farm-school children in south africa: a randomized controlled study.a randomized controlled trial was conducted to assess the effect of african leafy vegetable (alv) consumption on fe, zn and vitamin a status in children.201626146039
microbacterium horti sp. nov., a bacterium isolated from cucurbita maxima cultivating soil.a novel bacterial strain thg-sl1(t) was isolated from a soil sample of cucurbita maxima garden and was characterized by using a polyphasic approach. cells were gram-reaction-positive, non-motile and rod-shaped. the strain was aerobic, catalase positive and weakly positive for oxidase. phylogenetic analysis based on 16s rrna gene sequence analysis but it shared highest similarity with microbacterium ginsengisoli kctc 19189(t) (96.6 %), indicating that strain thg-sl1(t) belongs to the genus microb ...201626757723
can adverse effects of acidity and aluminum toxicity be alleviated by appropriate rootstock selection in cucumber?low-ph and aluminum (al) stresses are the major constraints that limit crop yield in acidic soils. grafting vegetable elite cultivars onto appropriate rootstocks may represent an effective tool to improve crop tolerance to acidity and al toxicity. two greenhouse hydroponic experiments were performed to evaluate growth, yield, biomass production, chlorophyll index, electrolyte leakage, mineral composition, and assimilate partitioning in plant tissues of cucumber plants (cucumis sativus l. "ekron" ...201627621740
comparative proteomics of cucurbit phloem indicates both unique and shared sets of proteins.cucurbits are well-studied models for phloem biology but unusually possess both fascicular phloem (fp) within vascular bundles and additional extrafascicular phloem (efp). although the functional differences between the two systems are not yet clear, sugar analysis and limited protein profiling have established that fp and efp have divergent compositions. here we report a detailed comparative proteomics study of fp and efp in two cucurbits, pumpkin and cucumber. we re-examined the sites of exuda ...201627472661
the introduction of submitted approaches in iranian traditional medicine for treatment of diabetes.diabetes mellitus is a complex disorder of carbohydrate metabolism. according to past studies, the word ziabites in iranian traditional medicine (itm) correlate with diabetes. the aim of this study was to identify the definition, therapeutics, and mechanism of diabetes from the resources of itm in order to provide new recommendations for the treatment of diabetes.201627840534
no accumulation alleviates h2 o2 -dependent oxidative damage induced by ca(no3 )2 stress in the leaves of pumpkin-grafted cucumber seedlings.nitric oxide (no) and hydrogen peroxide (h2 o2 ), two important signaling molecules, are stimulated in plants by abiotic stresses. in this study, we investigated the role of no and its interplay with h2 o2 in the response of self-grafted (s-g) and salt-tolerant pumpkin-grafted (cucurbita maxima × c. moschata) cucumber seedlings to 80 mm ca(no3 )2 stress. endogenous no and h2 o2 production in s-g seedlings increased in a time-dependent manner, reaching maximum levels after 24 h of ca(no3 )2 stres ...201627935073
how do modern pesticide treatments influence the mobility of old incurred ddt contaminations in agricultural soils?even more than 50 years after the ban of ddt in germany, farmers are still affected by its persistence in contaminated soils. depending on the crop cultivated on such soils, this often leads to low-level residues of ddt and its metabolites dde and ddd ("ddx"), which are perceived as a risk by the food value chain. pesticide formulations used in modern agriculture commonly contain high levels of surfactants, but so far no open-field studies have evaluated the effects of these treatments on the mo ...201627662464
the introduction of submitted approaches in iranian traditional medicine for treatment of diabetes.diabetes mellitus is a complex disorder of carbohydrate metabolism. according to past studies, the word ziabites in iranian traditional medicine (itm) correlate with diabetes. the aim of this study was to identify the definition, therapeutics, and mechanism of diabetes from the resources of itm in order to provide new recommendations for the treatment of diabetes.201627516697
effects of dietary fiber extracted from pumpkin (cucurbita maxima duch.) on the physico-chemical and sensory characteristics of reduced-fat frankfurters.in this study, we investigated the effects of reducing fat levels from 30% to 25, 20, and 15% by substituting pork fat with water and pumpkin fiber (2%) on the quality of frankfurters compared with control. decreasing the fat concentration from 30% to 15% significantly increased moisture content, redness of meat batter and frankfurter, cooking loss, and water exudation, and decreased fat content, energy value, ph, and lightness of meat batter and frankfurter, hardness, cohesiveness, gumminess, c ...201627433101
discovering metabolic indices for early detection of squash (cucurbita maxima) storage quality using gc-ms-based metabolite profiling.squash (cucubita maxima) cultivars with good storage qualities are needed for breeding to improve poor crop supply during winter in japan. we measured changes in squash constituents during different storage periods to identify compounds that were suitable to be used as indices of storage quality. principal components analysis of compounds at 1-5 months after harvest showed that pc1 scores were lower for cultivars with a higher rather than lower sq (storage quality) ranks. partial least-squares r ...201626593601
physico-chemical properties and fatty acid composition of pomegranate, cherry and pumpkin seed oils.nut and seed oils are often considered waste products but in recent years they have been receiving growing interest due to their high concentration of hydrophilic and lipophilic bioactive components, which have important pharmacological properties on human health. the aim of this work was to compare the physico-chemical and biochemical properties of pomegranate (punicagranatum), sweet cherry (prunusavium) and pumpkin (cucurbita maxima) seed oils obtained by solvent extraction.201626033409
systemic induction of no-, redox-, and cgmp signaling in the pumpkin extrafascicular phloem upon local leaf wounding.cucurbits developed the unique extrafascicular phloem (efp) as a defensive structure against herbivorous animals. mechanical leaf injury was previously shown to induce a systemic wound response in the efp of pumpkin (cucurbita maxima). here, we demonstrate that the phloem antioxidant system and protein modifications by no are strongly regulated during this process. activities of the central antioxidant enzymes dehydroascorbate reductase, glutathione reductase and ascorbate reductase were rapidly ...201626904092
tannic acid alleviates bulk and nanoparticle nd2o3 toxicity in pumpkin: a physiological and molecular response.the effect of dissolved organic matter (dom) on nanoparticle toxicity to plants is poorly understood. in this study, tannic acid (ta) was selected as a dom surrogate to explore the mechanisms of neodymium oxide nps (nd2o3 nps) phytotoxicity to pumpkin (cucurbita maxima). the results from the tested concentrations showed that 100 mg l(-1) nd2o3 nps were significantly toxic to pumpkin in term of fresh biomass, and the similar results from the bulk particles and the ionic treatments were also evide ...201627308847
comparative analysis of cucurbita pepo metabolism throughout fruit development in acorn squash and oilseed pumpkin.both the fruit mesocarp and the seeds of winter squash can be used for consumption, although the focus of breeding efforts varies by cultivar. cultivars bred for fruit consumption are selected for fruit mesocarp quality traits such as carotenoid content, percent dry matter, and percent soluble solids, while these traits are essentially ignored in oilseed pumpkins. to compare fruit development in these two types of squash, we sequenced the fruit transcriptome of two cultivars bred for different p ...201627688889
influence of light during storage on composition of blue hubbard squash. 201618878042
carotenemia associated with green bean ingestion.carotenemia is a condition characterized by yellow discoloration of the skin and elevated blood carotene levels. excessive and prolonged ingestion of carotene-rich, yellow- or orange-colored foods such as carrots and winter squash is the most common cause, but more rarely it may be associated with consumption of other foods as well as with hypothyroidism, diabetes mellitus, anorexia nervosa, liver disease, or kidney disease. though not uncommon in children, there are few reports in the pediatric ...201615575851
the bionomics of the cocoa mealybug, exallomochlus hispidus (morrison) (hemiptera: pseudococcidae), on mangosteen fruit and three alternative hosts.the cocoa mealybug, exallomochlus hispidus morrison (hemiptera: pseudococcidae) is known to attack mangosteen, an important fruit export commodity for indonesia. the mealybug is polyphagous, so alternative host plants can serve as a source of nourishment. this study aimed to record the bionomics of e. hispidus on mangosteen (garcinia mangostana l.) and three alternative hosts, kabocha squash (cucurbita maxima l.), soursop (annona muricata, l.), and guava (psidium guajava l.). first-instar nymphs ...201728757558
glucose content and in vitro bioaccessibility in sweet potato and winter squash varieties during storage.glucose content and in vitro bioaccessibility were determined in raw and cooked pulp of arapey, cuabé, and beauregard sweet potato varieties, as well as maravilla del mercado and atlas winter squash, after zero, two, four, and six months of storage (14 °c, 80% relative humidity (rh)). the total glucose content in 100 g of raw pulp was, for arapey, 17.7 g; beauregard, 13.2 g; cuabé, 12.6 g; atlas, 4.0 g; and in maravilla del mercado, 4.1 g. these contents were reduced by cooking process and stora ...201728665302
salinity tolerance of grafted watermelon seedlings.in order to evaluate the salinity tolerance of grafted watermelon, two sets of experiments were conducted in a growing chamber where 'esmeralda' varieties were grafted onto interspecific squash (cucurbita maxima duch. × cucurbita moschata duch.) and lagenaria siceraria rootstocks. both non-grafted and self-garfted plants were used for control. for salt stress, 2.85 and 4.28 mm/l substrate doses of nacl were added with each irrigation in 2 day intervals for a duration of 23 days. interspecific-gr ...201729262705
detection of new viruses in alfalfa, weeds and cultivated plants growing adjacent to alfalfa fields in saudi arabia.a total of 1368 symptomatic plant samples showing different virus-like symptoms such as mottling, chlorosis, mosaic, yellow mosaic, vein clearing and stunting were collected from alfalfa, weed and cultivated plant species growing in vicinity of alfalfa fields in five principal regions of alfalfa production in saudi arabia. das-elisa test indicated occurrence of 11 different viruses in these samples, 10 of which were detected for the first time in saudi arabia. eighty percent of the alfalfa sampl ...201728855829
resurgence of cucurbit downy mildew in the united states: insights from comparative genomic analysis of pseudoperonospora cubensis.pseudoperonospora cubensis, the causal agent of cucurbit downy mildew (cdm), is known to exhibit host specialization. the virulence of different isolates of the pathogen can be classified into pathotypes based on their compatibility with a differential set composed of specific cucurbit host types. however, the genetic basis of host specialization within p. cubensis is not yet known. total genomic dna extracted from nine isolates of p. cubensis collected from 2008 to 2013 from diverse cucurbit ho ...201728861228
identification and genetic characterization of ralstonia solanacearum species complex isolates from cucurbita maxima in china.ralstonia solanacearum species complex is a devastating phytopathogen with an unusually wide host range, and new host plants are continuously being discovered. in june 2016, a new bacterial wilt on cucurbita maxima was observed in guangdong province, china. initially, in the adult plant stage, several leaves of each plant withered suddenly and drooped; the plant then wilted completely, and the color of their vasculature changed to dark brown, ultimately causing the entire plant to die. creamy-wh ...201729093727
anti obese potential of cucurbita maxima seeds oil: effect on lipid profile and histoarchitecture in high fat diet induced obese rats.in this study, we made an attempt to evaluate the potential of cucurbita maxima seeds oil (cso) against high-fat diet (hfd)-induced obesity in rats. we investigated the effect of cso (100 mg/kg body weight) supplementation over 30 days on the changes of hfd-induced obese rats in body weight, biochemical parameters and lipid profile as well as investigated the effects of cso on the histopathological changes. oral administration with cso revealed significant diminution in body weight gain, glucose ...201729047298
evidence based study of antidiabetic potential of c. maxima seeds - in vivo.in vitro antidiabetic efficacy of cucurbita maxima seed extract (cmse) has already been studied in our previous findings. thus, in order to validate these findings in biological system, in vivo antidiabetic activity of aqueous extract was investigated in normal as well as diabetic experimental models.201729034194
evaluation of three pumpkin species: correlation with physicochemical, antioxidant properties and classification using spme-gc-ms and e-nose methods.to ascertain the most discriminant variables for three pumpkin species principal component analysis (pca) was performed. twenty-four parameters (ph, conductivity, sucrose, glucose, total soluble solids, l*, a*, b*, individual weight, edible rate, firmness, citric acid, fumaric acid, l-ascorbic acid, malic acid, ppo activity, pod activity, total flavonoids, vitamin e, total phenolics, dpph, frap, β-carotene, and aroma) were considered. the studied pumpkin species were cucurbita maxima, cucurbita ...201728974797
karyotype stability and unbiased fractionation in the paleo-allotetraploid cucurbita genomes.the cucurbita genus contains several economically important species in the cucurbitaceae family. here, we report high-quality genome sequences of c. maxima and c. moschata and provide evidence supporting an allotetraploidization event in cucurbita. we are able to partition the genome into two homoeologous subgenomes based on different genetic distances to melon, cucumber, and watermelon in the benincaseae tribe. we estimate that the two diploid progenitors successively diverged from benincaseae ...201728917590
isolation of protein storage vacuoles and their membranes.protein-storage vacuoles (psvs) are specialized vacuoles that sequester large amounts of storage proteins. during seed development, psvs are formed de novo and/or from preexisting lytic vacuoles. seed psvs can be subdivided into four distinct compartments: membrane, globoid, matrix, and crystalloid. in this chapter, we introduce easy methods for isolation of psvs and their membranes from pumpkin seeds. these methods facilitate the identification and characterization of psv components.201727730610
dechlorination and chlorine rearrangement of 1,2,5,5,6,9,10-heptachlorodecane mediated by the whole pumpkin seedlings.short chain chlorinated paraffins (sccps) are ubiquitously present as persistent organic pollutants in the environment. however, little information on the interaction of sccps with plants is currently available. in this work, young pumpkin plants (cucurbita maxima × c. moschata) were hydroponically exposed to the congener of chlorinated decane, 1,2,5,5,6,9,10-heptachlorodecane (1,2,5,5,6,9,10-hepcd), to investigate the uptake, translocation and transformation of chlorinated decanes in the intact ...201728242253
fruit quality of seedless watermelon grafted onto squash rootstocks under different production systems.the market demand for seedless watermelon has been continuously increasing because of consumer preference. grafting is a useful tool to manage soil-borne diseases in watermelon production, but the use of squash rootstocks may negatively affect watermelon fruit quality. currently, most research has focused on seeded cultivars while grafting effects on seedless watermelons remain largely unknown. this multi-season study was conducted to assess the effects of squash rootstocks, including both cucur ...201728369915
coccinia indica agglutinin, a 17kda pp2 like phloem lectin: affinity purification, primary structure and formation of self-assembled filaments.phloem protein-2 (pp2) is an abundant soluble protein in the sieve elements in plants. its lectin property was reported in various species. the primary structure of a 17kda pp2 from coccinia indica (coccinia indica agglutinin, cia17), determined by mass spectrometry, shows extensive homology with pp2 super family phloem lectins. analysis of mass spectrometric data indicated the presence of 16 potential allelic variants of cia17 with insignificant divergence in the primary structure. the primary ...201829126937
uptake, translocation and biotransformation of n-ethyl perfluorooctanesulfonamide (n-etfosa) by hydroponically grown plants.n-ethyl perfluorooctane sulfonamide (n-etfosa) is an important perfluorooctanesulfonate (pfos) precursor (prefos) which is used in sulfluramid. the present work studied the uptake, translocation and metabolism of n-etfosa in wheat (triticum aestivum l.), soybean (glycine max l. merrill) and pumpkin (cucurbita maxima l.) by hydroponic exposure. except for parent n-etfosa, its metabolites of perfluorooctane sulfonamide acetate (fosaa), perfluorooctane sulfonamide (pfosa), pfos, perfluorohexane sul ...201829306808
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