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antisense suppression of an acid invertase gene (mai1) in muskmelon alters plant growth and fruit development.to unravel the roles of soluble acid invertase in muskmelon (cucumis melo l.), its activity in transgenic muskmelon plants was reduced by an antisense approach. for this purpose, a 1038 bp cdna fragment of muskmelon soluble acid invertase was expressed in antisense orientation behind the 35s promoter of the cauliflower mosaic virus. the phenotype of the antisense plants clearly differed from that of control plants. the transgenic plant leaves were markedly smaller, and the stems were obviously t ...200818641398
a new strain of melon necrotic spot virus that is unable to systemically infect cucumis melo.we report a new strain of melon necrotic spot virus (mnsv) that is unable to systemically infect cucumis melo. a spherical virus (w-isolate), about 30 nm in diameter like a carmovirus, was isolated from watermelons with necrotic symptoms. the w-isolate had little serological similarity to mnsv, and it did not cause any symptoms in six melon cultivars susceptible to mnsv; however, the host range of the w-isolate was limited exclusively to cucurbitaceous plants, and transmission by o. bornovanus w ...200818943404
recovery from cucurbit leaf crumple virus (family geminiviridae, genus begomovirus) infection is an adaptive antiviral response associated with changes in viral small rnas.a strong recovery response occurs in cantaloupe (cucumis melo) and watermelon (citrullus lanatus) infected with the bipartite begomovirus cucurbit leaf crumple virus (culcrv). this response is characterized by initially severe symptoms, which gradually become attenuated (almost symptomless). an inverse relationship was detected between viral dna levels and recovery, indicating that recovered tissues had reduced viral titers. recovered tissues also were resistant to reinfection with culcrv; i.e., ...200818943741
possible amelioration of atherogenic diet induced dyslipidemia, hypothyroidism and hyperglycemia by the peel extracts of mangifera indica, cucumis melo and citrullus vulgaris fruits in rats.hitherto unknown efficacy of the peel extracts of mangifera indica (mi), cucumis melo (cm) and citrullus vulgaris (cv) fruits in ameliorating the diet-induced alterations in dyslipidemia, thyroid dysfunction and diabetes mellitus have been investigated in rats. in one study, out of 4 different doses (50-300 mg/kg), 200 mg/kg of mi and 100 mg/kg for other two peel extracts could inhibit lipidperoxidation (lpo) maximally in liver. in the second experiment rats were maintained on pre-standardized a ...200819276533
alternative sanitizers to chlorine for use on fresh-cut "galia" (cucumis melo var. catalupensis) melon.chlorine is commonly used to reduce microbial load in fresh-cut vegetables. however, the production of chlorinated organic compounds, such as trihalomethanes, which are potential carcinogens, has created the need to investigate the efficiency of nontraditional sanitizers and alternative techniques. the effects of 4 novel sanitizers were tested in fresh-cut "galia" melon: chlorine dioxide (clo(2)) at 3 mg/l, peracetic acid (paa) at 80 mg/l, hydrogen peroxide (h(2)o(2)) at 50 mg/l, and nisin at 25 ...200819021810
long-distance trafficking of macromolecules in the phloem.the fact that macromolecules such as proteins and mrnas overcome the symplastic barriers between various tissue domains was first evidenced by the movement of plant viruses. we have recently demonstrated that viral infection disengages the symplastic restriction present between the sieve element-companion cell complex and neighboring cells in tobacco plants. as a result, green fluorescent protein, which was produced in mesophyll and bundle sheath cells, could traffic into the sieve tube and trav ...200819704648
construction of a fosmid library of cucumber (cucumis sativus) and comparative analyses of the eif4e and eif(iso)4e regions from cucumber and melon (cucumis melo).a fosmid library of cucumber was synthesized as an unrestricted resource for researchers and used for comparative sequence analyses to assess synteny between the cucumber and melon genomes, both members of the genus cucumis and the two most economically important plants in the family cucurbitaceae. end sequencing of random fosmids produced over 680 kilobases of cucumber genomic sequence, of which 25% was similar to ribosomal dnas, 25% to satellite sequences, 20% to coding regions in other plants ...200818273646
ethylene production in plants during transformation suppresses vir gene expression in agrobacterium tumefaciens.ethylene evolution from plants inhibits agrobacterium-mediated genetic transformation, but the mechanism is little understood. in this study, the possible role of ethylene in agrobacterium-mediated genetic transformation was clarified. it was tested whether or not plant ethylene sensitivity affected genetic transformation; the sensitivity might regulate bacterial growth during co-cultivation and vir gene expression in agrobacterium tumefaciens. for these experiments, melon (cucumis melo) was use ...200818331427
3-methylthiopropionic acid ethyl ester, isolated from katsura-uri (japanese pickling melon, cucumis melo var. conomon), enhanced differentiation in human colon cancer cells.the fully ripened fruit of katsura-uri japanese pickling melon ( cucumis melo var. conomon) has rarely been used for food because the midripened fruit is utilized for making pickles, but the fully ripened fruit is no longer valuable for pickles due to the fruit body being too soft. we have considered the utilization of the fully ripened katsura-uri fruit that may be used for nonpickling products, particularly if the fully ripened fruit demonstrated health benefits such as anticarcinogenic proper ...200818426216
antioxidant, sugar, mineral, and phytonutrient concentrations across edible fruit tissues of orange-fleshed honeydew melon (cucumis melo l.).orange-fleshed, non-netted honeydew ( cucumis melo l.) is a relatively new melon in the marketplace and has shown a lot of potential as an alternative to netted muskmelons (cantaloupes), which are often prone to surface contamination by enteric bacteria. orange-fleshed honeydew is a cross between orange-fleshed cantaloupe and non-netted, green-fleshed honeydew. this glasshouse study investigated the nutritional profile (phytonutrient and sugar contents) in different tissues of mature orange-fles ...200818454549
reduction by gaseous ozone of salmonella and microbial flora associated with fresh-cut cantaloupe.this research investigates the efficacy of gaseous ozone, applied under partial vacuum in a controlled reaction chamber, for the elimination of salmonella inoculated on melon rind. the performance of high dose, short duration treatment with gaseous ozone, in this pilot system, on the microbial and sensory quality of fresh-cut cantaloupes was also evaluated. gaseous ozone (10,000 ppm for 30 min under vacuum) reduced viable, recoverable salmonella from inoculated physiologically mature non-ripe an ...200818456110
survival of sa11 rotavirus in fresh fruit juices of pineapple, papaya, and honeydew melon.survival of rotavirus in fresh fruit juices of papaya (caraca papaya l.), honeydew melon (cucumis melo l.), and pineapple (ananas comosus [l.] merr.) was studied. clarified juices were prepared from pulps of ripe fruits and sterilized by ultrafiltration. one milliliter of juice from each fruit was inoculated with 20 microl of 1 x 10(6) pfu of sa11 rotavirus and sampled immediately (0-h exposure) and 1 and 3 h later at 28 degrees c. mean viral titers in juices of papaya (ph 5.1) and honeydew melo ...200818522042
effect of gaseous ozone and hot water on microbial and sensory quality of cantaloupe and potential transference of escherichia coli o157:h7 during cutting.the effect of gaseous ozone and hot water, alone or in combination, on the sensory and microbial quality of cantaloupe melon was investigated. escherichia coli o157:h7 transmission from the rind to edible melon flesh during cutting practices was also investigated. four different treatments consisting of hot water (75 degrees c, 1min), gaseous ozone (10,000ppm, 30min), gaseous ozone supplied by carbon monoxide gas and the combination of hot water and gaseous ozone were evaluated. sensory quality ...200817993390
mapping of genetic loci that regulate quantity of beta-carotene in fruit of us western shipping melon (cucumis melo l.).melon (cucumis melo l.) is highly nutritious vegetable species and an important source of beta-carotene (vitamin a), which is an important nutrient in the human diet. a previously developed set of 81 recombinant inbred lines (ril) derived from group cantalupensis us western shipper market type germplasm was examined in two locations [wisconsin (wi) and california (ca), usa] over 2 years to identify quantitative trait loci (qtl) associated with quantity of beta-carotene (qbetac) in mature fruit. ...200818773190
occurrence and distribution of ten viruses infecting cucurbit plants in guilan province, iran.during the 2006 and 2007 growing seasons, a systematic survey was conducted in open-field of melon (cucumis melo l.), cucumber (c. sativus l.), squash (cucurbita sp.), and watermelon (citrulus lanatus l.) crops in 16 major cucurbit-growing areas of guilan province in iran. symptomatic leaf samples were collected and screened by double-antibody sandwich elisa (das-elisa) or rt-pcr to detect zucchini yellow mosaic virus (zymv), watermelon mosaic virus (wmv), cucurbit aphid-borne yellows virus (cab ...200818564898
comparative histochemical analyses of oxidative burst and cell wall reinforcement in compatible and incompatible melon-powdery mildew (podosphaera fusca) interactions.the spatial-temporal expression patterns of oxidative burst and cell wall reinforcement were analyzed in leaves of resistant and susceptible melon (cucumis melo l.) cultivars in response to podosphaera fusca (fr.) braun & shishkoff, the main causal agent of powdery mildew in cucurbits. extensive development of powdery mildew mycelia and a progressive increase in haustorial count were recorded in the susceptible cultivar after 4d, while in the resistant cultivar powdery mildew failed to grow and ...200818585824
salmonella litchfield outbreak associated with a hotel restaurant--atlantic city, new jersey, 2007.on july 10, 2007, the pennsylvania department of health notified the new jersey department of health and senior services (njdhss) of three culture-confirmed cases of salmonella litchfield infection with matching pulsed-field gel electrophoresis (pfge) patterns. data from pulsenet, the national molecular subtyping network for foodborne disease surveillance, confirmed 11 cases (including the three from pennsylvania) of this rarely identified salmonella serotype in five states during a 5-week perio ...200818636066
distribution and pathway for phloem-dependent movement of melon necrotic spot virus in melon plants.the translocation of melon necrotic spot virus (mnsv) within tissues of inoculated and systemically infected cucumis melo l. 'galia' was studied by tissue-printing and in situ hybridization techniques. the results were compatible with the phloem vascular components being used to spread mnsv systemically by the same assimilate transport route that runs from source to sink organs. virus rnas were shown to move from the inoculated cotyledon toward the hypocotyl and root system via the external phlo ...200818705860
edible alginate-based coating as carrier of antimicrobials to improve shelf-life and safety of fresh-cut melon.the effect of malic acid and essential oils (eos) of cinnamon, palmarosa and lemongrass and their main active compounds as natural antimicrobial substances incorporated into an alginate-based edible coating on the shelf-life and safety of fresh-cut "piel de sapo" melon (cucumis melo l.) was investigated. melon pieces (50 g) were coated with alginate-based edible coating containing malic acid (ec) and eos or their active compounds before to be packed in air filled polypropylene trays and stored a ...200818164505
complete nucleotide sequence of a new isolate of tomato leaf curl new delhi virus infecting cucumber, bottle gourd and muskmelon in thailand. 200818193155
enzymatic detection of mercuric ions in ground-water from vegetable wastes by immobilizing pumpkin (cucumis melo) urease in calcium alginate beads.present report describes a quick and simple test based on enzyme inhibition for the detection of mercury in aqueous medium by urease immobilized in alginate beads. urease was extracted from the discarded seeds of pumpkin (cucumis melo) and was purified to apparent homogeneity (5.2-fold) by heat treatment at 48+/-0.1 degrees c and gel filtration through sephadex g-200. the homogeneous enzyme preparation (sp activity 353 u/mg protein, a(280)/a(260)=1.12) was immobilized in 3.5% alginate leading to ...200817933526
molecular authentication of three italian melon accessions by arms-pcr and its1 (internal transcribed spacer 1) secondary structure prediction.genetic assessment was carried out on three italian melon accessions by sequence and structural analysis of the internal transcribed spacer 1 (its1) from three populations belonging to two cucumis melo l. varieties (madras and tendral). alignment of the 18s-5.8s-26s sequences from three melon accessions showed that there were three single-nucleotide polymorphisms (snps) and one short insertion-deletion (indel) at the 5'end its1. an amplification refractory mutation system (arms)-pcr-based analys ...200818478086
factorial design for the optimization of enzymatic detection of cadmium in aqueous solution using immobilized urease from vegetable waste.free as well as alginate immobilized urease was utilized for detection and quantitation of cadmium (cd2+) in aqueous samples. urease from the seeds of pumpkin (cucumis melo), being a vegetable waste, was extracted and purified to apparent homogeneity (sp. activity 353 u/mg protein; a280/a260=1.12) by heat treatment at 48+/-0.1 degrees c and gel filtration through sephadex g-200. the homogeneous enzyme preparation was immobilized in 3.5% alginate leading to 86% immobilization and no leaching of t ...200818378444
melon fruits: genetic diversity, physiology, and biotechnology features.among cucurbitaceae, cucumis melo is one of the most important cultivated cucurbits. they are grown primarily for their fruit, which generally have a sweet aromatic flavor, with great diversity and size (50 g to 15 kg), flesh color (orange, green, white, and pink), rind color (green, yellow, white, orange, red, and gray), form (round, flat, and elongated), and dimension (4 to 200 cm). c. melo can be broken down into seven distinct types based on the previously discussed variations in the species ...200818322855
bin mapping of genomic and est-derived ssrs in melon (cucumis melo l.).we report the development of 158 primer pairs flanking ssr motifs in genomic (gssr) and est (est-ssr) melon sequences, all yielding polymorphic bands in melon germplasm, except one that was polymorphic only in cucurbita species. a similar polymorphism level was found among est-ssrs and gssrs, between dimeric and trimeric est-ssrs, and between est-ssrs placed in the open reading frame or any of the 5'- or 3'-untranslated regions. correlation between ssr length and polymorphism was only found for ...200818806992
identification of qtls for resistance to powdery mildew and ssr markers diagnostic for powdery mildew resistance genes in melon (cucumis melo l.).powdery mildew caused by podosphaera xanthii is an important foliar disease in melon. to find molecular markers for marker-assisted selection, we constructed a genetic linkage map of melon based on a population of 93 recombinant inbred lines derived from crosses between highly resistant ar 5 and susceptible 'earl's favourite (harukei 3)'. the map spans 877 cm and consists of 167 markers, comprising 157 simple sequence repeats (ssrs), 7 sequence characterized amplified region/cleavage amplified p ...200818797839
characterization and role of polyphenol oxidase and peroxidase in browning of fresh-cut melon.polyphenol oxidase (ppo) and peroxidase (pod) were extracted from two different varieties of melon ( cucumis melo l. cantalupensis cv. charentais and c. melo l. inodorus cv. amarillo) and characterized using reliable spectrophotometric methods. in both cases the enzymes followed michaelis-menten kinetics, showing different values of kinetics parameters between the two cultivars: k m = 7.18 +/- 0.70 mm ('charentais') and 6.66 +/- 0.20 mm ('amarillo') mm; v max = 7.93 +/- 0.35 units/min ('charenta ...200818069784
reme1, a copia retrotransposon in melon, is transcriptionally induced by uv light.for the first time, numerous sequences of copia and gypsy retrotransposons from the cucumis melo genome have been obtained and analyzed. phylogenetic analyses of sequences of both types of long terminal repeat (ltr) retrotransposons were carried out. the melon genome contains approximately 20,000 gypsy and 6,800 copia elements, comprising about 26% of its total size. starting from a retrotransposon fragment, we have cloned and characterized an entire melon retrotransposon, named reme1, which is ...200818034313
effect of gamma rays on different explants of callus treatment of multiple shoots in cucumis melo cv. bathasa.a mutagenesis programme was carried out using physical mutagens (gamma rays) on cucumis melo cv. bathasa. in irradiated seeds the number of shoots formed in the lower doses was significantly higher than the controls. decrease in the number of shoots and shoot bud formation was observed with higher doses in all the explants studies. there was complete lethality in the 10, 15 and 20 kr doses. in irradiated stem cultures the maximum number of shoots were observed in 2 kr. the number of shoots decre ...200819295084
employment of pumpkin (cucumis melo) urease entrapped in alginate beads for quantitation of cadmium in aqueous media.the potential of employment of free as well as alginate-immobilized urease for the quantitation of cadmium (cd(2+)) was explored. urease from the seeds of pumpkin (cucumis melo) was purified to apparent homogeneity by heat treatment at 48 +/- 0.1 degrees c and gel filtration through sephadex g-200. the purified enzyme exhibited a single band on native page under coomassie brilliant blue and silver staining. the enzyme entrapped in 3.5% alginate beads (with 86% immobilization) exhibited no leachi ...200818830881
on the genetic control of heterosis for fruit shape in melon (cucumis melo l.).the objective of the present work is to study the genetic basis of heterosis for fruit shape (fs) in melon observed in a cross between the spanish cultivar "piel de sapo" (ps) and the korean accession pi 161375 (songwang charmi [sc]) using a set of near-isogenic lines (nils) with contrasting phenotypes for fs, each carrying a single chromosomal introgression from sc within the genetic background of ps. we investigated the fs of homozygous nils, hybrids nil x ps, and all 2-way crosses between nil ...200918815117
cucurbitane-type triterpenoids from the stems of cucumis melo.phytochemical investigation of the stems of cucumis melo led to the isolation and identification of 21 cucurbitane-type triterpenoids, including nine new compounds (1-9) and 12 known compounds. their structures were determined on the basis of spectroscopic analyses, chemical methods, and comparison with spectroscopic data in the literature. two known compounds, cucurbitacin b (10) and cucurbitacin a (11), showed significant cytotoxic activity against the proliferation of a549/atcc and bel7402 ce ...200919348465
1h nmr, gc-ei-tofms, and data set correlation for fruit metabolomics: application to spatial metabolite analysis in melon.a metabolomics approach combining (1)h nmr and gas chromatography-electrospray ionization time-of-flight mass spectrometry (gc-ei-tofms) profiling was employed to characterize melon (cucumis melo l.) fruit. in a first step, quantitative (1)h nmr of polar extracts and principal component analyses (pca) of the corresponding data highlighted the major metabolites in fruit flesh, including sugars, organic acids, and amino acids. in a second step, the spatial localization of metabolites was investiga ...200919298059
identification of host attractants for the ethiopian fruit fly, dacus ciliatus loew.the ethiopian fruit fly, dacus ciliatus, is an oligophagous pest of cucurbit crops, particularly melons, cucumbers, and marrows (summer squash). the present study aimed to identify host attractants for d. ciliatus and was guided by a behavioral bioassay and an electrophysiological assay. we tested volatile compounds from the fruits of a host plant, ripe and unripe galia melon, cucumis melo var. reticulates. both sexes were attracted to melon volatiles. those of ripe melon were preferred. gas chr ...200919440796
the genome of the cucumber, cucumis sativus l.cucumber is an economically important crop as well as a model system for sex determination studies and plant vascular biology. here we report the draft genome sequence of cucumis sativus var. sativus l., assembled using a novel combination of traditional sanger and next-generation illumina ga sequencing technologies to obtain 72.2-fold genome coverage. the absence of recent whole-genome duplication, along with the presence of few tandem duplications, explains the small number of genes in the cuc ...200919881527
an oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (cucumis melo l.).melon (cucumis melo) is a horticultural specie of significant nutritional value, which belongs to the cucurbitaceae family, whose economic importance is second only to the solanaceae. its small genome of approx. 450 mb coupled to the high genetic diversity has prompted the development of genetic tools in the last decade. however, the unprecedented existence of a transcriptomic approaches in melon, highlight the importance of designing new tools for high-throughput analysis of gene expression.200919821986
occurrence of fuligo gyrosa causing slime mold of oriental melon.recently, a severe slime mold infestation affected oriental melon plants in fields in chilgok county, gyeongbuk province, korea. specimens were collected from the fields and examined for identification. a species of myxomycetes, fuligo gyrosa, was identified based on its morphological characteristics. this is the first report that f. gyrosa causes slime mold of oriental melon.200923983540
centromere repositioning in cucurbit species: implication of the genomic impact from centromere activation and inactivation.the centromere of an eukaryotic chromosome can move to a new position during evolution, which may result in a major alteration of the chromosome morphology and karyotype. this centromere repositioning phenomenon has been extensively documented in mammalian species and was implicated to play an important role in mammalian genome evolution. here we report a centromere repositioning event in plant species. comparative fluorescence in situ hybridization mapping using common sets of fosmid clones bet ...200919706458
reproductive biology of the andromonoecious cucumis melo subsp. agrestis (cucurbitaceae).cucumis melo subsp. agrestis (cucurbitaceae) is cultivated in many african regions for its edible kernels used as a soup thickener. the plant, an annual, andromonoecious, trailing-vine species, is of high social, cultural and economic value for local communities. in order to improve the yield of this crop, the first step and our aim were to elucidate its breeding system.200919671577
a set of est-snps for map saturation and cultivar identification in melon.there are few genomic tools available in melon (cucumis melo l.), a member of the cucurbitaceae, despite its importance as a crop. among these tools, genetic maps have been constructed mainly using marker types such as simple sequence repeats (ssr), restriction fragment length polymorphisms (rflp) and amplified fragment length polymorphisms (aflp) in different mapping populations. there is a growing need for saturating the genetic map with single nucleotide polymorphisms (snp), more amenable for ...200919604363
superoxide dismutase activity in mesocarp tissue from divergent cucumis melo l. genotypes.muskmelons (cucumis melo l.) are well-known as excellent sources of several vitamins, minerals and non-enzymatic antioxidant phytochemicals such as vitamin c and pro-vitamin a. less well-studied is their potential role as sources of enzymatic antioxidants such as superoxide dismutase (sod), which have been associated with enhanced reactive oxygen species scavenging capacity in some muskmelon fruits. in this study, we investigated the variability in sod activities among diverse advanced breeding ...200919529987
occurrence of fruit rot of melon caused by sclerotium rolfsii in korea.in 2007 to 2008, a fruit rot of melon (cucumis melo l.) caused by sclerotium rolfsii occurred sporadically in a farmer's vinyl house in jinju city. the symptoms started with watersoaking lesion and progressed into the rotting of the surface of fruit. white mycelial mats appeared on the lesion at the surface of the fruit and a number of sclerotia formed on the fruit near the soil line. the sclerotia were globoid in shape, 1~3 mm in size, and white to brown in color. the hyphal width was measured ...200923983527
nutritional composition, microbiological and sensory properties of dried melon: a traditional turkmen product.seven different cultivars (taze vaharman, gökjek, arap hatli, türkmen vaharmani, torli payendeke, yazki bişek, gizil gülabi) of honeydew melons (cucumis melo l.) from turkmenistan were processed to a dried traditional product known as 'kavun kaki' in order to evaluate the most suitable cultivar for drying process. the chemical composition (dry matter, crude oil, crude protein, crude fibre, energy, ash, water-soluble solids, total sugar, invert sugar, titratable acidity, ph and mineral contents) ...200918608557
cloning and characterization of a soluble acid invertase-encoding gene from muskmelon.soluble acid invertase (s-aiv; ec 3.2.1.26) is thought to play a critical role in sucrose hydrolysis in muskmelon (cucumis melo l.) fruit. a full-length cdna clone encoding s-aiv was isolated from muskmelon by rt-pcr and race. the clone, designated as cms-aiv1, contains 2178 nucleotides with an open reading frame of 1908 nucleotides. the deduced 636 amino acid sequence showed high identities with other plant soluble acid invertases. northern blot analysis indicated that cms-aiv1 was expressed in ...200918317948
the cucurbitaceae and solanaceae illustrated in medieval manuscripts known as the tacuinum sanitatis.beginning in the last two decades of the 14th century, richly illuminated versions of the tacuinum sanitatis, the latin translation of an 11th-century arabic manuscript known as taqwim al-sihha bi al-ashab al-sitta, were produced in northern italy. these illustrated manuscripts provide a window on late medieval life in that region by containing some 200 full-page illustrations, many of which vividly depict the harvest of vegetables, fruits, flowers, grains, aromatics and medicinal plants. our ob ...200919318382
species and genus specificity of the intergenic spacer (igs) in the ribosomal rna genes of cucurbitaceae.the use of intergenic spacer (igs) fragments of plant ribosomal dna (rdna) for the differentiation between genera and species is tested by cross-hybridization experiments with different igs probes of two cucurbitaceae, cucurbita pepo (zucchini) and cucumis sativus (cucumber). hybridization with cloned fragments of different parts of the igs of ribosomal dna exhibit a different degree of conservation within and between the cucurbitaceae genera. in general, cucurbita species seem to be closer rela ...20092631730
molecular cloning, characterization and expression analysis of cmapx.the rt pcr and race methods were used to obtain the cdna sequence of an apx gene of muskmelon after the leaves were induced with powdery mildew. the cdna length of the apx gene is 1,047 bp with a 750 bp orf encoded a 249 amino acid and the molecular weight of apx protein is 27.3 kda. the analysis showed that the cmapx genomic dna contained 10 extrons and 9 introns. the identity of the amino acid sequence deduced from the cdna with the apx family of other homologous members was about 74-97%. a fu ...200918756374
interactions of bacillus spp. and plants--with special reference to induced systemic resistance (isr).biological control of soil-borne pathogens comprises the decrease of inoculum or of the disease producing activity of a pathogen through one or more mechanisms. interest in biological control of soil-borne plant pathogens has increased considerably in the last few decades, because it may provide control of diseases that cannot or only partly be managed by other control strategies. recent advances in microbial and molecular techniques have significantly contributed to new insights in underlying m ...200918845426
a multi-state outbreak of salmonella saintpaul in australia associated with cantaloupe consumption.a multi-state outbreak of salmonella enterica serovar saintpaul infection occurred in australia during october 2006. a case-control study conducted in three affected jurisdictions, new south wales, victoria and australian capital territory, included 36 cases with the outbreak-specific strain of s. saintpaul identified by multiple locus variable-number tandem repeat analysis (mlva) in a faecal specimen and 106 controls. consumption of cantaloupe (rockmelon) was strongly associated with illness (a ...200918559128
virus-specific read-through codon preference affects infectivity of chimeric cucumber green mottle mosaic viruses displaying a dengue virus epitope.a cucumber green mottle mosaic virus (cgmmv) was used to present a truncated dengue virus type 2 envelope (e) protein binding region from amino acids 379 to 423 (eb4). the eb4 gene was inserted at the terminal end of the cgmmv coat protein (cp) open reading frame (orf). read-through sequences of tmv or cgmmv, caa-uag-caa-uua, or aaa-uag-caa-uua were, respectively, inserted in between the cp and the eb4 genes. the chimeric clones, prt, prg, and pcg+fsrtre, were transcribed into full-length capped ...200919325913
generation of transgenic oriental melon resistant to zucchini yellow mosaic virus by an improved cotyledon-cutting method.production of melon (cucumis melo l.) worldwide is often limited by the potyvirus, zucchini yellow mosaic virus (zymv). in order to engineer melon lines resistant to zymv, a construct containing the translatable coat protein (cp) sequence coupled with the 3' non-translatable region of the virus was generated and used to transform an elite cultivar of oriental melon (silver light) mediated by agrobacterium using an improved cotyledon-cutting method. removal of 1-mm portion from the proximal end o ...200919479262
sensitivity to azoxystrobin in didymella bryoniae isolates collected before and after field use of strobilurin fungicides.isolates of didymella bryoniae (auersw.) rehm, causal agent of gummy stem blight on cucurbits, developed insensitivity to azoxystrobin in the eastern united states 2 years after first commercial use in 1998. baseline sensitivity of this fungus to azoxystrobin has never been reported. the objectives were to compare baseline sensitivities of d. bryoniae from south carolina and other locations to sensitivities of isolates exposed to azoxystrobin for one or more seasons, and to compare sensitivity i ...200919488995
a consensus linkage map identifies genomic regions controlling fruit maturity and beta-carotene-associated flesh color in melon (cucumis melo l.).the nutritional value and yield potential of us western shipping melon (usws; cucumis melo l.) could be improved through the introgression of genes for early fruit maturity (fm) and the enhancement of the quantity of beta-carotene (qbetac) in fruit mesocarp (i.e., flesh color). therefore, a set of 116 f(3) families derived from the monoecious, early fm chinese line 'q 3-2-2' (no beta-carotene, white mesocarp) and the andromonoecious, late fm usws line 'top mark' (possessing beta-carotene, orange ...200919551368
a conserved ethylene biosynthesis enzyme leads to andromonoecy in two cucumis species.andromonoecy is a widespread sexual system in angiosperms, characterized by plants carrying both male and bisexual flowers. monoecy is characterized by the presence of both male and female flowers on the same plant. in cucumber, these sexual forms are controlled by the identity of the alleles at the m locus. in melon, we recently showed that the transition from monoecy to andromonoecy result from a mutation in 1-aminocyclopropane-1-carboxylic acid synthase (acs) gene, cmacs-7. to isolate the and ...200919578542
curtovirus-cucurbit interaction: acquisition host plays a role in leafhopper transmission in a host-dependent manner.curly top disease (ctd) of vegetable crops is caused by viruses in the genus curtovirus (family geminiviridae). cucurbits are reported to be susceptible to ctd; however, the disease is rare in california despite annual outbreaks in other hosts (e.g., common bean, pepper, sugar beet, and tomato). consistent with these observations, no obvious curly top symptoms were observed in melon fields surveyed for ctd in central california in 2004 and 2005, whereas the disease was readily observed in tomato ...200919055441
protective role of mangifera indica, cucumis melo and citrullus vulgaris peel extracts in chemically induced hypothyroidism.an investigation was made to evaluate the pharmacological importance of fruit peel extracts of mangifera indica (mi), citrullus vulgaris (cv) and cucumis melo (cm) with respect to the possible regulation of tissue lipid peroxidation (lpo), thyroid dysfunctions, lipid and glucose metabolism. pre-standardized doses (200mg/kg of mi and 100mg/kg both of cv and cm), based on the maximum inhibition in hepatic lpo, were administered to wistar albino male rats for 10 consecutive days and the changes in ...200919059228
cadmium accumulation by muskmelon under salt stress in contaminated organic soil.human-induced salinization and trace element contamination are widespread and increasing rapidly, but their interactions and environmental consequences are poorly understood. phytoaccumulation, as the crucial entry pathway for biotoxic cd into the human foodstuffs, correlates positively with rhizosphere salinity. hypothesising that organic matter decreases the bioavailable cd(2+) pool and therefore restricts its phytoextraction, we assessed the effects of four salinity levels (0, 20, 40 and 60 m ...200919162301
hypersensitive response to aphis gossypii glover in melon genotypes carrying the vat gene.aphis gossypii glover causes direct and indirect damage to cucumis melo l. crops. to decrease the harmful effects of this pest, one of the most economically and environmentally acceptable options is to use genetically resistant melon varieties. to date, several sources of resistance carrying the vat gene are used in melon breeding programmes that aim to prevent a. gossypii colonization and the subsequent aphid virus transmission. the results suggest that the resistance conferred by this gene is ...200919474089
influence of thermal and dense-phase carbon dioxide pasteurization on physicochemical properties and flavor compounds in hami melon juice.the influence of thermal and dense-phase carbon dioxide (dp-co(2)) pasteurization on physicochemical properties and flavor compounds in hami melon juice was investigated. melon juice was pasteurized using dp-co(2) treatment and compared to a conventional high-temperature-short-time (htst) method. the dp-co(2) treatment was carried out using a dp-co(2) unit (55 degrees c, 60 min, and 35 mpa). the thermal pasteurization was performed at 90 degrees c for 60 s with an adapted laboratory setup. effec ...200919522496
polyploidization facilitates biotechnological in vitro techniques in the genus cucumis.prezygotic interspecific crossability barrier in the genus cucumis is related to the ploidy level of the species (cucumber (c. sativus), x = 7; muskmelon (c. melo) and wild cucumis species, x = 12). polyploidization of maternal plants helps hybridization among other cucumis species by overcoming prezygotic genetic barriers. the main objective of this paper is to compare the results of several methods supporting interspecific crosses in cucumber without and with polyploidization (comparison betwe ...201021234406
effects of combined heat and acetic acid on natural microflora reduction on cantaloupe melons.produce is an important source of nutrients and phytochemicals, which is important in a healthy diet. however, perishable fresh produce has caused recent outbreaks of foodborne diseases. high level of nutrients and water activity, direct contact with soil, and lack of thermal procedures during primary processing make fresh produce a potential food safety hazard. fruits and vegetables with rough surfaces can harbor microorganisms and support their multiplication, increasing the risk of this hazar ...201020501053
role of leaf glandular trichomes of melon plants in deterrence of aphis gossypii glover.external characteristics of the leaf epidermis and their effects on behaviour of aphis gossypii glover were evaluated in two cucumis melo l. genotypes, 'bola de oro' (aphid susceptible) and tgr-1551 (aphid resistant) in order to explore their role in the early rejection of tgr-1551 by this aphid. no differential effects of epicuticular waxes on aphid behaviour were observed. the type, distribution and number of trichomes on melon leaves were also studied. pubescence in melon, measured as the num ...201020522187
chlorotic curly stunt: a severe begomovirus disease of bottle gourd in northern india.bottle gourd (lagenaria siceraria) an important vegetable crop in india was observed to be affected by a chlorotic curly stunt disease (ccsd) during 2003-2006 in the vegetable growing areas of delhi and adjoining state of haryana. the affected plants are severely stunted and bear very small chlorotic and mildly curled leaves. incidence of the disease varied from 4.7 to 36%. the disease could be easily transmitted by whitefly, bemisia tabaci but not by sap. the causal virus was found to be a bego ...201023637479
soft rot on cucumis melo var. makuwa caused by rhizopus oryzae.rhizopus oryzae is reported for the first time on cucumis melo l. var. makuwa makino. a detailed description of this korean specimen is given, along with its rdna internal transcribed spacer sequence. on the basis of mycological characteristics and molecular data, the fungus was identified as r. oryzae went & prinsen geerligs.201023956677
antisense repression of sucrose phosphate synthase in transgenic muskmelon alters plant growth and fruit development.to unravel the roles of sucrose phosphate synthase (sps) in muskmelon (cucumis melo l.), we reduced its activity in transgenic muskmelon plants by an antisense approach. for this purpose, an 830 bp cdna fragment of muskmelon sucrose phosphate synthase was expressed in antisense orientation behind the 35s promoter of the cauliflower mosaic virus. the phenotype of the antisense plants clearly differed from that of control plants. the transgenic plant leaves were markedly smaller, and the plant hei ...201020138160
field experiments of anopheles gambiae attraction to local fruits/seedpods and flowering plants in mali to optimize strategies for malaria vector control in africa using attractive toxic sugar bait methods.based on recent studies in israel demonstrating that attractive toxic sugar bait (atsb) methods can be used to decimate local anopheline and culicine mosquito populations, an important consideration is whether the same methods can be adapted and improved to attract and kill malaria vectors in africa. the atsb approach uses fruit or flower scent as an attractant, sugar solution as a feeding stimulant, and an oral toxin. the atsb solutions are either sprayed on vegetation or suspended in simple ba ...201020854666
[impacts of root-zone hypoxia stress on muskmelon growth, its root respiratory metabolism, and antioxidative enzyme activities].by using aeroponics culture system, this paper studied the impacts of root-zone hypoxia (10% o2 and 5% o2) stress on the plant growth, root respiratory metabolism, and antioxidative enzyme activities of muskmelon at its fruit development stage. root-zone hypoxia stress inhibited the plant growth of muskmelon, resulting in the decrease of plant height, root length, and fresh and dry biomass. comparing with the control (21% o2), hypoxia stress reduced the root respiration rate and malate dehydroge ...201020873618
extraordinary resistance to insecticides reveals exotic q biotype of bemisia tabaci in the new world.a strain of the whitefly bemisia tabaci (gennadius) possessing unusually high levels of resistance to a wide range of insecticides was discovered in 2004 in the course of routine resistance monitoring in arizona. the multiply resistant insects, collected from poinsettia (euphorbia pulcherrima willd. ex klotzsch) plants purchased at a retail store in tucson, were subjected to biotype analysis in three laboratories. polyacrylamide gel electrophoresis of naphthyl esterases and sequencing of the mit ...201021309242
food reservoir for escherichia coli causing urinary tract infections.closely related strains of escherichia coli have been shown to cause extraintestinal infections in unrelated persons. this study tests whether a food reservoir may exist for these e. coli. isolates from 3 sources over the same time period (2005-2007) and geographic area were compared. the sources comprised prospectively collected e. coli isolates from women with urinary tract infection (uti) (n = 353); retail meat (n = 417); and restaurant/ready-to-eat foods (n = 74). e. coli were evaluated for ...201020031048
branched-chain and aromatic amino acid catabolism into aroma volatiles in cucumis melo l. fruit.the unique aroma of melons (cucumis melo l., cucurbitaceae) is composed of many volatile compounds biosynthetically derived from fatty acids, carotenoids, amino acids, and terpenes. although amino acids are known precursors of aroma compounds in the plant kingdom, the initial steps in the catabolism of amino acids into aroma volatiles have received little attention. incubation of melon fruit cubes with amino acids and alpha-keto acids led to the enhanced formation of aroma compounds bearing the ...201020065117
efficient dna extraction from nail clippings using the protease solution from cucumis melo.owing to the increasing importance of genomic information, obtaining genomic dna easily from biological specimens has become more and more important. this article proposes an efficient method for obtaining genomic dna from nail clippings. nail clippings can be easily obtained, are thermostable and easy to transport, and have low infectivity. the drawback of their use, however, has been the difficulty of extracting genomic material from them. we have overcome this obstacle using the protease solu ...201020306236
host range and complete genome sequence of cucurbit chlorotic yellows virus, a new member of the genus crinivirus.cucurbit chlorotic yellows virus (ccyv) causes chlorotic yellows on cucumber (cucumis sativus) and melon (cucumis melo) and is transmitted by bemisia tabaci biotype b and q whiteflies. to characterize the host range of ccyv, 21 cucurbitaceous and 12 other plant species were inoculated using whitefly vectors. all tested cucumis spp. except cucumis anguria and cucumis zeyheri were systemically infected with ccyv, although infection rates varied among species. citrullus lanatus, cucurbita pepo, and ...201020465411
rapid detection of squash leaf curl virus by loop-mediated isothermal amplification.a loop-mediated isothermal amplification (lamp) assay was employed to develop a simple and efficient system for the detection of squash leaf curl virus (slcv) in diseased plants of squash (cucurbita pepo) and melon (cucumis melo). completion of lamp assay required 30-60 min under isothermal conditions at 65 degrees c by employing a set of four primers targeting slcv. although the sensitivity of the lamp assay and the polymerase chain reaction (pcr) assay was comparable at high virus concentratio ...201020603151
the levels of male gametic mitochondrial dna are highly regulated in angiosperms with regard to mitochondrial inheritance.the mechanisms that regulate mitochondrial inheritance are not yet clear, even though it is 100 years since the first description of non-mendelian genetics. here, we quantified the copy numbers of mitochondrial dna (mtdna) in the gametic cells of angiosperm species. we demonstrate that each egg cell from arabidopsis thaliana, antirrhinum majus, and nicotiana tabacum possesses 59.0, 42.7, and 73.0 copies of mtdna on average, respectively. these values are equivalent to those in arabidopsis mesoph ...201020605854
functional analysis of the cucumisin propeptide as a potent inhibitor of its mature enzyme.cucumisin is a subtilisin-like serine protease (subtilase) that is found in the juice of melon fruits (cucumis melo l.). it is synthesized as a preproprotein consisting of a signal peptide, nh(2)-terminal propeptide, and 67-kda protease domain. we investigated the role of this propeptide (88 residues) in the cucumisin precursor. complementary dnas encoding the propeptides of cucumisin, two other plant subtilases (arabidopsis ara12 and rice rsp1), and bacterial subtilisin e were expressed in esch ...201020639575
cucumber (cucumis sativus) and melon (c. melo) have numerous wild relatives in asia and australia, and the sister species of melon is from australia.among the fundamental questions regarding cultivated plants is their geographic origin and region of domestication. the genus cucumis, which includes cucumber (cucumis sativus) and melon (cucumis melo), has numerous wild african species, and it has therefore been assumed that melon originated in africa. for cucumber, this seemed less likely because wild cucumbers exist in india and a closely related species lives in the eastern himalayas. using dna sequences from plastid and nuclear markers for ...201020656934
antimutagenic; differentiation-inducing; and antioxidative effects of fragrant ingredients in katsura-uri (japanese pickling melon; cucumis melo var. conomon).six fragrant ingredients were identified in fully-ripened katsura-uri (japanese pickling melon; cucumis melo var. conomon). four of them were sulfur-containing compounds [methylthioacetic acid ethyl ester (mtae), acetic acid 2-methylthio ethyl ester (amte), 3-methylthiopropionic acid ethyl ester (mtpe), and acetic acid 3-methylthio propyl ester (amtp)]; and the others were benzyl acetate and eugenol. the newly identified mtae and amtp possessed antimutagenic activity as determined by their abili ...201020801232
genetics of flavonoid, carotenoid, and chlorophyll pigments in melon fruit rinds.external color has profound effects on acceptability of agricultural products by consumers. carotenoids and chlorophylls are known to be the major pigments of melon (cucumis melo l.) rinds. flavonoids (especially chalcones and anthocyanins) are also prominent in other fruits but have not been reported to occur in melons fruit. we analyzed the pigments accumulating in rinds of different melon genotypes during fruit development. we found that melon rind color is based on different combinations of ...201020815398
a k+ channel from salt-tolerant melon inhibited by na+.• the possible roles of k(+) channels in plant adaptation to high na(+) conditions have not been extensively analyzed. here, we characterize an inward shaker k(+) channel, mirk (melon inward rectifying k(+) channel), cloned in a salt-tolerant melon (cucumis melo) cultivar, and show that this channel displays an unusual sensitivity to na(+) . • mirk expression localization was analyzed by reverse-transcription pcr (rt-pcr). mirk functional analyses were performed in yeast (growth tests) and xenop ...201021077888
isolation and characterization of a gs2 gene in melon (cucumis melo l.) and its expression patterns under the fertilization of different forms of n.we isolated a novel glutamine synthetase (gs, ec 6.3.1.2) gene m-gs2 (accession: ay773090) by the race approach from melon. the full-length cdna of m-gs2 is 1807 bp and contains a 1296 bp open reading frame (orf) encoding 432 amino acids. the deduced protein contains conserved structural domains among plant gs2 proteins and shares extensive sequence homology with gs2 enzymes from other higher plants. m-gs2 expresses with specificity in leaf, and identification of a chloroplast transit peptide (c ...201019669951
cloning and characterization of a sucrose synthase-encoding gene from muskmelon.a full-length cdna clone encoding sucrose synthase (ss; ec 2.4.1.13) was isolated from muskmelon (cucumis melo l.) by rt-pcr and race. the clone, designated as cmss1, contains 2,585 nucleotides with an open reading frame of 2,412 nucleotides. the deduced 804 amino acid sequence showed high identities with other plant sucrose synthase. real time pcr analysis indicated that cmss1 expression differed among root, stem, leaf, flower and fruit tissues. the analysis during fruit development indicated t ...201019415524
identification and characterization of the causal organism of gummy stem blight in the muskmelon (cucumis melo l.).gummy stem blight is a major foliar disease of muskmelon (cucumis melo l.). in this study, morphological characteristics and rdna internal transcribed spacer (its) sequences were analyzed to identify the causal organism of this disease. morphological examination of the jeonbuk isolate revealed that the percentage of monoseptal conidia ranged from 0% to 10%, and the average length × width of the conidia was 70 (± 0.96) × 32.0 (± 0.15) µm on potato dextrose agar. the blast analysis showed nucleoti ...201023956648
functional identification of arabidopsis atsip2 (at3g57520) as an alkaline α-galactosidase with a substrate specificity for raffinose and an apparent sink-specific expression pattern.arabidopsis atsip2 has recently been suggested to be a raffinose synthase gene. however, it has high amino acid identity to functionally characterized alkaline α-galactosidases from cucumis melo and zea mays. using the sf9 insect cell expression system, we demonstrate that recombinant atsip2 is a genuine alkaline α-galactosidase with a distinct substrate specificity for raffinose, and not a raffinose synthase. a β-glucuronidase reporter construct using the atsip2 promoter shows that atsip2 is st ...201020739305
influence of genotype and explant source on the in vitro regeneration ability of different melon varieties.nine genotypes of melon (cucumis melo l.) were selected for the investigation of regeneration. most of the tested varieties showed regeneration ability on medium containing 0.5 mg l⁻¹ or 1 mg l⁻¹ ba, but following the appearance of shoot buds, only six varieties produced leafy shoots. the effect of combinations of ba with different auxins (iaa, na, 2,4-d) and aba in the culture medium on shoot regeneration was tested on cotyledon explants of 'hógolyó' and 'hale's best'. to establish optimal cond ...201021112840
sequencing of 6.7 mb of the melon genome using a bac pooling strategy.cucumis melo (melon) belongs to the cucurbitaceae family, whose economic importance among horticulture crops is second only to solanaceae. melon has a high intra-specific genetic variation, morphologic diversity and a small genome size (454 mb), which make it suitable for a great variety of molecular and genetic studies. a number of genetic and genomic resources have already been developed, such as several genetic maps, bac genomic libraries, a bac-based physical map and est collections. sequenc ...201021073723
genome-wide bac-end sequencing of cucumis melo using two bac libraries.although melon (cucumis melo l.) is an economically important fruit crop, no genome-wide sequence information is openly available at the current time. we therefore sequenced bac-ends representing a total of 33,024 clones, half of them from a previously described melon bac library generated with restriction endonucleases and the remainder from a new random-shear bac library.201021054843
[genetic diversity of a germplasm collection of cucumis melo l. using srap markers].genetic relationships and classifications of a set of melon accessions were analyzed to provide the experimental support for utilizing effectively genetic materials for breeding. sequence-related amplified polymorphism technique was adopted to analyze 61 melon accessions. sixteen primer combinations with clear band pattern and polymorphism were selected from over 42 primer combinations. four hundred and fifty-two loci were detected by 16 pairs of srap primers. among them, 265 were polymorphic, t ...201020650857
[expression analysis of the unisex-determine gene m in cucumber].sex determination in plants has various mechanism; however, a single gene locus controlling unisexual expression is unique in the cucurbitaceae plants, particularly cucumber (cucumis sativus l.) and melon (cucumis melo l.). in this study, with quantitative rt-pcr method, two sets of near-isogenic lines (nils) were used to analyze the expression pattern of gene csacs2 (genbank accession number fj529216). additionally, chemical applications (agno3 and avg) were used to investigate the effect of th ...201020566468
karyotyping in melon (cucumis melo l.) by cross-species fosmid fluorescence in situ hybridization.chromosome identification is critical for cytogenetic research and will accelerate studies on genetic variation and breeding, especially for those species with relatively little sequence information. so far, no reliable cytological landmarks have been developed to distinguish individual chromosomes in melon. in this study, using fish (fluorescence in situ hybridization) combined with comparative genome information, we selected 21 cucumber fosmids anchored by ssr markers as chromosome-specific cy ...201020551614
generation of a bac-based physical map of the melon genome.cucumis melo (melon) belongs to the cucurbitaceae family, whose economic importance among horticulture crops is second only to solanaceae. melon has high intra-specific genetic variation, morphologic diversity and a small genome size (450 mb), which make this species suitable for a great variety of molecular and genetic studies that can lead to the development of tools for breeding varieties of the species. a number of genetic and genomic resources have already been developed, such as several ge ...201020509895
new 2-(2-phenylethyl)chromone derivatives from the seeds of cucumis melo l var. reticulatus.chemical investigation of the methanolic extract of the seeds of cucumis melo l. var. reticulatus (cucurbitaceae) afforded three new chromone derivatives; 5,7-dihydroxy-2-[2-(4-hydroxyphenyl)ethyl]chromone 3, 5,7-dihydroxy-2-[2-(3,4-dihydroxyphenyl)ethyl]chromone 4, and 7-glucosyloxy-5-hydroxy-2-[2-(4-hydroxyphenyl)ethyl]chromone 6, together with three known compounds; beta-amyrin 1, beta-sitosterol 2, and beta-sitosterol-3-o-beta-glucopyranoside 5. their structures were established by uv, ir, 1 ...201020420317
a genetic map of melon highly enriched with fruit quality qtls and est markers, including sugar and carotenoid metabolism genes.a genetic map of melon enriched for fruit traits was constructed, using a recombinant inbred (ri) population developed from a cross between representatives of the two subspecies of cucumis melo l.: pi 414723 (subspecies agrestis) and 'dulce' (subspecies melo). phenotyping of 99 ri lines was conducted over three seasons in two locations in israel and the us. the map includes 668 dna markers (386 ssrs, 76 snps, six indels and 200 aflps), of which 160 were newly developed from fruit ests. these est ...201020401460
steering nitrogen fertilisation by means of portable chlorophyll meter reduces nitrogen input and improves quality of fertigated cantaloupe (cucumis melo l. var. cantalupensis naud.).farming is considered one of the main causes of land degradation and underground water pollution. the increased availability of agricultural inputs has led to a dramatic rise in yields, which has resulted in soil fertility spoilage and overuse of fertilisers. therefore horticultural practice improvement must consider appropriate nitrogen (n) management. this paper reports results on the application of an optical diagnostic system (n-tester) to guide n fertilisation in muskmelon (cucumis melo l.) ...201020355071
transformation of a marker-free and vector-free antisense acc oxidase gene cassette into melon via the pollen-tube pathway.melons have short shelf-lives due to fruit ripening caused by ethylene production. the 1-aminocyclopropane-1-carboxylic acid (acc) oxidase gene is essential for ethylene biosynthesis. as fruit ripening in other fruit crops can be deterred by down-regulation of acc oxidase expression, we have carried out similar work to improve fruit quality and shelf-life of the melon cucumis melo. a marker-free and vector-free antisense 1-aminocyclopropane-1-carboxylic acid oxidase construct was transformed int ...201120865442
structural basis for reduced activity of 1-aminocyclopropane-1-carboxylate synthase affected by a mutation linked to andromonoecy.1-aminocyclopropane-1-carboxylate synthase (acs) is a key enzyme in the biosynthesis of the plant hormone ethylene. recently, a new biological role for acs has been found in cucumis melo where a single point mutation (a57v) of one isoform of the enzyme, causing reduced activity, results in andromonoecious plants. we present here a straightforward structural basis for the reduced activity of the a57v mutant, based on our work on malus domestica acs, including a new structure of the unliganded app ...201121075107
a new australian species of luffa (cucurbitaceae) and typification of two australian cucumis names, all based on specimens collected by ferdinand mueller in 1856.as a result of his botanical explorations in northern australia, ferdinand von mueller named several cucurbitaceae that molecular data now show to be distinct, requiring their resurrection from unjustified synonymy. we here describe and illustrate luffa saccata f. muell. ex i.telford, validating a manuscript name listed under luffa graveolens roxb. since 1859, and we lectotypify cucumis picrocarpus f. muell. and cucumis jucundus f. muell. the lectotype of the name cucumis jucundus, a synonym of ...201122171190
grafting helps improve photosynthesis and carbohydrate metabolism in leaves of muskmelon.the most important quality for muskmelon (cucumis melo l.) is their sweetness which is closely related to the soluble sugars content. leaves are the main photosynthetic organs in plants and thus the source of sugar accumulation in fruits since sugars are translocated from leaves to fruits. the effects of grafting muskmelon on two different inter-specific (cucurbita maxima×c. moschata) rootstocks was investigated with respect to photosynthesis and carbohydrate metabolism. grafting zhongmi1 muskme ...201122043173
evaluation of cucurbitacin-based gustatory stimulant to facilitate cucumber beetle (coleoptera: chrysomelidae) management with foliar insecticides in melons.the bitter plant-derived compounds cucurbitacins are known to stimulate feeding of adult cucumber beetles (coleoptera: chrysomelidae). a cucurbitacin-based gustatory stimulant applied as a flowable bait combined with either spinosad or carbaryl was compared with foliar sprays of spinosad and carbaryl for controlling two cucumber beetle species (diabrotica undecimpunctata undecimpunctata mannerheim and acalymma trivittatum mannerheim) in honeydew melons (cucumis melo l.). field studies were condu ...201121882695
analysis of the melon (cucumis melo) small rnaome by high-throughput pyrosequencing.melon (cucumis melo l.) is a commercially important fruit crop that is cultivated worldwide. the melon research community has recently benefited from the determination of a complete draft genome sequence and the development of associated genomic tools, which have allowed us to focus on small rnas (srnas). these are short, non-coding rnas 21-24 nucleotides in length with diverse physiological roles. in plants, they regulate gene expression and heterochromatin assembly, and control protection agai ...201121812964
a consensus linkage map for molecular markers and quantitative trait loci associated with economically important traits in melon (cucumis melo l.).a number of molecular marker linkage maps have been developed for melon (cucumis melo l.) over the last two decades. however, these maps were constructed using different marker sets, thus, making comparative analysis among maps difficult. in order to solve this problem, a consensus genetic map in melon was constructed using primarily highly transferable anchor markers that have broad potential use for mapping, synteny, and comparative quantitative trait loci (qtl) analysis, increasing breeding e ...201121797998
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