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inteins in mycobacterial gyra are a taxonomic character.the a subunit of dna gyrase in mycobacteria is frequently subjected to splicing events as its gene, gyra, harbours an insertion encoding an intein. investigation of a number of different isolates of mycobacterium kansasii, mycobacterium malmoense, mycobacterium marinum, mycobacterium ulcerans and mycobacterium xenopi demonstrated that the presence of gyra inteins is not random but a taxonomic character specific for a given taxon at a species or subspecies level.19989493395
verrucous nodule of the finger. 19989521036
differentiation of mycobacterium ulcerans, m. marinum, and m. haemophilum: mapping of their relationships to m. tuberculosis by fatty acid profile analysis, dna-dna hybridization, and 16s rrna gene sequence analysis.although mycobacterium ulcerans, m. marinum, and m. haemophilum are closely related, their exact taxonomic placements have not been determined. we performed gas chromatography of fatty acids and alcohols, as well as dna-dna hybridization and 16s rrna gene sequence analysis, to clarify their relationships to each other and to m. tuberculosis. m. ulcerans and m. marinum were most closely related to one another, and each displayed very strong genetic affinities to m. tuberculosis; they are actually ...19989542909
goldfish, carassius auratus, a novel animal model for the study of mycobacterium marinum pathogenesis.we have developed an animal model for studying mycobacterial pathogenesis using mycobacterium marinum and the goldfish, carassius auratus. goldfish are injected intraperitoneally with doses between 10(2) and 10(9) cfu of m. marinum organisms. depending on the dose of m. marinum organisms administered, an acute or chronic disease is produced. the acute disease is characterized by systemic mycobacterial infection, severe peritonitis, tissue necrosis, and a short median survival time. the chronic d ...19989596770
concurrent infection due to shewanella putrefaciens and mycobacterium marinum acquired at the beach.infection with marine bacteria is uncommon. a patient with systemic lupus erythematosus who developed concurrent infection with shewanella putrefaciens and mycobacterium marinum (m. marinum) is described. after bathing leg ulcers in sea water, severe cellulitis of the left leg with necrotic areas and extensive bullae developed. infection due to s. putrefaciens was confirmed and a long course of hospitalization, oral ciprofloxacin and skin grafting was required. during hospitalization subcutaneou ...19989611378
mycobacterium marinum wrist arthritis: local and systematic dissemination caused by concomitant immunosuppressive therapy.the diagnostic difficulties and the potential serious course of mycobacterium marinum synovitis are illustrated in a patient in whom treatment with local steroid injections and immunosuppressive therapy resulted in local destruction of the wrist and systemic reactions. healing was achieved after correct medical and surgical treatment was initiated.19989670365
electron microscope studies of the in vitro phagocytosis of mycobacterium spp. by rainbow trout oncorhynchus mykiss head kidney macrophages.the cytological response of rainbow trout oncorhynchus mykiss head kidney macrophages to ingested mycobacterium spp. was examined in vitro. mycobacterium marinum or mycobacterium sp. tb267 isolated from snakehead fish channa striata bloch were opsonised with either fresh rainbow trout serum, serum which had been heat-inactivated, or rainbow trout antiserum against the extracellular products (ecp) of the 2 mycobacterium spp. a monoclonal antibody against the ecp was also used as an opsonin. suspe ...19989676251
oxidative stress response and characterization of the oxyr-ahpc and fura-katg loci in mycobacterium marinum.oxidative stress response in pathogenic mycobacteria is believed to be of significance for host-pathogen interactions at various stages of infection. it also plays a role in determining the intrinsic susceptibility to isoniazid in mycobacterial species. in this work, we characterized the oxyr-ahpc and fura-katg loci in the nontuberculous pathogen mycobacterium marinum. in contrast to mycobacterium smegmatis and like mycobacterium tuberculosis and mycobacterium leprae, m. marinum was shown to pos ...19989733688
mycobacterium marinum: chronic and extensive infections of the lower limbs in south pacific islanders.we report three adult cases of very chronic, extensive infection of the lower limbs due to mycobacterium marinum. the patients were from south pacific islands and, clinically, the widespread warty plaques resembled chromomycosis. one was associated with severe lymphoedema. all three patients gave a history of at least 20 years duration. the patients were otherwise well and not immunologically compromised. in all cases, the organism was identified on tissue cultures and was not seen on histopatho ...19989737045
the identification of mycobacterium marinum genes differentially expressed in macrophage phagosomes using promoter fusions to green fluorescent protein.mycobacterium marinum, like mycobacterium tuberculosis, is a slow-growing pathogenic mycobacteria that is able to survive and replicate in macrophages. using the promoter-capture vector pfpv27, we have constructed a library of 200-1000 bp fragments of m. marinum genomic dna inserted upstream of a promoterless green fluorescent protein (gfp) gene. only those plasmids that contain an active promoter will express gfp. macrophages were infected with this fusion library, and phagosomes containing flu ...19989767585
[mycobacterium marinum skin infections, apropos of a case]. 19989780437
[painful nodular lesions]. 19989793156
an unusual case of flexor sheath tenosynovitis.we report a case of a flexor sheath tenosynovitis caused by mycobacterium marinum.19989821624
septic arthritis of the hand and wrist.septic arthritis of the hand and wrist is relatively uncommon. the most common cause is penetrating trauma such as a human or animal bite. the most common causative organism is staphylococcus aureus. septic arthritis caused by streptococcal species. haemophilus influenzae, pseudomonas aeruginosa, serratia species, neisseria gonorrhoeae, pasteurella multocida, eikenella corrodens, and mycobacterium marinum also may occur in specific clinical settings. the best clinical results occur following an ...19989884896
atypical hand infections.the diagnosis and treatment of an atypical hand infection present a distinctive challenge for the hand surgeon. infections caused by these uncommon organisms occur more often in immunocompromised patients. this article emphasizes the salient features of mycobacterial, nocardial, and fungal infections of the hand. with an accurate and timely diagnosis, appropriate surgical and pharmacologic treatment may eradicate these unusual infections.19989884899
polymerase chain reaction-reverse cross-blot hybridization assay in the diagnosis of sporotrichoid mycobacterium marinum infection.in this paper, we report a patient in whom mycobacterium marinum sporotrichoid infection was diagnosed using polymerase chain reaction (pcr) amplification of the 16s rrna gene and subsequent analysis of the amplified product in a reverse cross-blot hybridization assay with mycobacterial species-specific probes. this molecular method allowed us rapidly to detect and identify this organism directly in the patient's lesional skin biopsy rather than in cultures in conventional media. the identificat ...19989892957
primary intravascular lymphomatosis associated with mycobacterium marinum.intravascular lymphomatosis (i.v.l.) is a rare condition in which neoplastic cells preferentially infiltrate blood vessels of the central nervous system. nonspecific symptoms associated with i.v.l. include dementia, seizures, and multifocal cerebrovascular events. i.v.l. was discovered at autopsy of a patient whose neurological deficits were predated by a particularly aggressive form of mycobacterium marinum soft-tissue infection. it is speculated that i.v.l. may have had an occult effect on the ...19989442593
pain and swelling along the nail fold of a 51-year-old man. 199810217741
[nontuberculous mycobacteria in the hospital environment].mycobacteria other than tubercle (mott) are ubiquitous. mycobacterial disease is most common at the extremes of age and at the patients with malignancy or hiv infection. in an attempt to establish effective measures to prevent nosocomial disease which has become increasingly important we investigated the presence of mott in hospital environment of oncological and pediatric units. between 1994-1997, 21 strains of mott were isolated from 500 environmental specimens (4.2% positive samples). the sou ...199810422317
sporotrichoid cutaneous infection caused by mycobacterium marinum. 199910440450
recurrent, disseminated mycobacterium marinum infection caused by the same genotypically defined strain in an immunocompromised patient.an 81-year-old male with myasthenia gravis developed a cutaneous infection with mycobacterium marinum, which apparently resolved following local heat therapy. five months later, the patient developed new skin lesions and pancytopenia. m. marinum was isolated from his bone marrow. pulsed-field gel electrophoresis was performed to determine if the skin and bone marrow isolates were clonally related. digestion of the genomic dna with the restriction enzymes spei and asei yielded indistinguishable b ...199910449508
identification of mycobacterial dna in cutaneous lesions of sarcoidosis.sarcoidosis is a multisystemic granulomatous disease of uncertain etiology. recently, mycobacterial dna especially mycobacterium tuberculosis and mycobacterium avium complex were detected in lung tissue and bronchial lavage fluid from patients with sarcoidosis by polymerase chain reaction (pcr) assays in 30% to 50% cases. moreover, cell wall-defective form (cwdf) acid-fast bacteria have been isolated from skin lesions of patients with sarcoidosis which were later confirmed as m. avium complex by ...199910472755
non-tuberculous mycobacterial tenosynovitis: a review.the clinical characteristics, outcome and treatment of non-tuberculous mycobacterial tenosynovitis are reviewed. from lesions localized in the hand, 10 different species of non-tuberculous mycobacteria have been reported. the most common are mycobacterium marinum and mycobacterium kansasii. other less frequent organisms are mycobacterium avium complex, mycobacterium szulgai, mycobacterium terrae, mycobacterium fortuitum, mycobacterium chelonae, mycobacterium abscessus, mycobacterium malmoense an ...199910482048
activation of human neutrophils by mycobacterial phenolic glycolipids.the interaction between mycobacterial phenolic glycolipids (pgls) and phagocytes was studied. human neutrophils were allowed to interact with each of four purified mycobacterial pgls and the neutrophil production of reactive oxygen metabolites was followed kinetically by luminol-/isoluminol-amplified chemiluminescence. the pgls from mycobacterium tuberculosis and mycobacterium kansasii, respectively, were shown to stimulate the production of oxygen metabolites, while pgls from mycobacterium mari ...199910540187
comparative antimycobacterial activities of ofloxacin, ciprofloxacin and grepafloxacin.infections caused by non-tuberculous mycobacteria and multidrug-resistant mycobacterium tuberculosis are difficult to treat. new compounds potentially active against these bacteria are therefore constantly being sought. among them is grepafloxacin, a new c5 fluoroquinolone. a panel of 130 isolates of mycobacteria including 33 m. tuberculosis isolates and 97 isolates of different species of atypical mycobacteria were analysed for susceptibility to grepafloxacin, ofloxacin and ciprofloxacin. the m ...199910552981
identification and semiquantitation of mycobacterium avium using a competitive pcr method.a competitive pcr method with standard dna (mimic) was developed for the rapid detection and semiquantitation of mycobacterium avium (m. avium) using primers specific for the alpha antigen sequence of the bacteria. dna from both m. avium and mycobacterium marinum was amplified by polymerase chain reaction (pcr), but only m. avium could be detected by subsequent blotting confirmation with a probe specific for the bacteria. with the pcr and subsequent dot blot hybridization, as little as 10 fg of ...199910553674
[sporotrichoid mycobacteriosis caused by mycobacterium marinum]. 199910572519
mycobacterium marinum keratitis: pigmentation a clue to diagnosis. 199910624441
picture of the month. mycobacterium marinum facial abscess. 199910323638
health and safety concerns in fisheries and aquaculture.only 10% of all foodborne illnesses in the united states are attributed to seafood, making seafood a relatively safe food commodity. the implementation of hazard analysis and critical control points guidelines should make seafood consumption even safer. concerns include closed-loop, indoor, water-recirculating production systems; harmful algal blooms in marine environments, which can cause paralytic, neurologic, amnesic, and diarrhetic shellfish poisonings and ciguatera fish poisoning; bacteria ...199910329911
atypical mycobacterial cutaneous infection. 199910330598
the mycobacterium marinum g13 promoter is a strong sigma 70-like promoter that is expressed in escherichia coli and mycobacteria species.a mycobacterium marinum promoter, designated g13, was isolated from a promoter-trap library as a constitutive producer of the mutant green fluorescent protein. sequence analysis, primer extension analysis, and computer promoter prediction analysis indicate that the g13 promoter is very similar to escherichia coli consensus sigma 70 promoters. expression of the green fluorescent protein from the g13 promoter in m. marinum is, however, up to 40 times higher than that seen from the mycobacterial hs ...199910361711
mycobacterium marinum with associated bursitis.mycobacterium marinum infections have been reported for over 50 years, mostly in association with trauma in the setting of water exposure.199910366400
superoxide production in phagocytes obtained from mycobacterium marinum-stimulated goldfish (carassius auratus) that were exposed to copper.to investigate the effects of copper exposure and recovery from copper toxicosis on the nonspecific immune response in mycobacterium marinum-inoculated goldfish.199910376889
mycobacterium marinum infection in a renal transplant recipient.infections with atypical mycobacteria occur more frequently in patients with solid organ transplants than in the normal host.199910385095
detection of mycobacteria in joint samples from patients with arthritis using a genus-specific polymerase chain reaction and sequence analysis.mycobacteria have been implicated in the pathogenesis of various forms of arthritis. the aim of this study was to examine the diagnostic potential of molecular biological techniques as well as to investigate the pathogenetic role of mycobacteria in chronic arthritis.199910402076
a finger infection in a pet store employee. 199910414641
comparative severity of experimentally induced mycobacteriosis in striped bass morone saxatilis and hybrid tilapia oreochromis spp.twenty striped bass morone saxatilis and 20 hybrid tilapia oreochromis niloticus x o. mossambicus x o. aureus each received a single intramuscular injection of 1.6 x 10(6) colony forming units per gram body weight of mycobacterium marinum. striped bass manifested significantly greater clinical and microscopic disease compared to tilapia. whereas all the striped bass had died or were clinically ill by day 8 post-infection, there was no apparent disruption of normal behaviour, physical appearance, ...199910686670
[long-standing skin nodular lesions in a 14-year-old male]. 199910687420
fish tank granuloma: response to treatment with clarithromycin. 199910188167
atypical mycobacterium infection with sporotrichoid spread in a patient with human immunodeficiency virus.a case of mycobacterium marinum infection presenting with a sporotrichoid spread in a hiv positive chinese male is presented. the patient responded to oral cotrimoxazole treatment. a brief review of the literature concerning atypical mycobacterial infection presenting in such a fashion is discussed.199910672400
[clinical analysis of 96 cases with pulmonary disease caused by nontuberculous mycobacteria].to study the clinical characteristics of nontuberculous mycobacteria (ntm) pulmonary disease.199911775923
mycobacterium marinum infections.mycobacterium marinum is a saprophytic mycobacteria capable of causing soft tissue infection in humans, usually acquired by inoculation. as with other mycobacterial infections, diagnosis may be difficult and recent developments in molecular biology are also being applied to atypical mycobacteria such as mycobacterium marinum.199917035776
analysis by enzyme-linked immunosorbent assay and immunoblot of the antibody responses of patients infected with mycobacterium marinum.the serum antibody responses of a total of 14 patients with active or recently cured mycobacterium marinum infections were analysed via a combination of enzyme-linked immunosorbent assay (elisa) and the immunodevelopment of western blots of m. marinum antigen. normal human sera and sera from patients with active pulmonary tuberculosis were also analysed as controls. the detectable igg response of m. marinum patients, as demonstrated by elisa, was highly variable and did not differ significantly ...200010683462
transformation and transposition of the genome of mycobacterium marinum.to develop and evaluate protocols for genetic manipulations (transformation and transposition) of the fish pathogen, mycobacterium marinum.200010685681
activation of cutaneous sarcoidosis following mycobacterium marinum infection of skin.sarcoidosis has long been associated with mycobacterial infection, especially with mycobacterium tuberculosis, although a causal association has not been proven. atypical mycobacteria have also been implicated in causing sarcoidosis, but there is as yet no conclusive evidence. we report the activation of cutaneous sarcoidosis following infection with m. marinum, raising further questions about the role of these bacteria in causing this chronic granulomatous disorder.200011204520
[cutaneous sporotricoid infection by mycobacterium marinum]. 200010721568
cutaneous infections due to nontuberculous mycobacteria: histopathological review of 28 cases. comparative study between lesions observed in immunosuppressed patients and normal hosts.to evaluate the histopathological features observed in patients with cutaneous infections due to nontuberculous mycobacteria (ntm) and to compare the histopathological patterns observed in immunosuppressed patients and normal hosts. twenty-eight biopsy specimens corresponding to 27 patients with cutaneous infections due to ntm were reviewed. eighteen biopsies corresponded to normal hosts (14 mycobacterium marinum, 2 mycobacterium chelonae, 1 mycobacterium terrae and 1 mycobacterium gordonae) and ...200010728814
mycobacteriosis in wild rabbitfish siganus rivulatus associated with cage farming in the gulf of eilat, red sea.infection patterns of mycobacterium marinum were studied over a period of 3 yr in wild rabbitfish siganus nivulatus populations associated with commercial mariculture cages and inhabiting various sites along the israeli red sea coastline. mycobacteriosis was first recorded from the red sea in 1990 in farmed sea bass dicentrarchus labrax and is absent from records of studies on parasites and diseases of wild rabbitfish carried out in the 1970s and 1980s. a sharp increase in the prevalence of the ...200010768289
granuloma-specific expression of mycobacterium virulence proteins from the glycine-rich pe-pgrs family.pathogenic mycobacteria, including the agent of tuberculosis, mycobacterium tuberculosis, must replicate in macrophages for long-term persistence within their niche during chronic infection: organized collections of macrophages and lymphocytes called granulomas. we identified several genes preferentially expressed when mycobacterium marinum, the cause of fish and amphibian tuberculosis, resides in host granulomas and/or macrophages. two were homologs of m. tuberculosis pe/pe-pgrs genes, a family ...200010827956
new clues to how the tb bacillus persists. 200010847835
retrospective study of mycobacterium marinum skin infections.although infection by mycobacterium marinum is well reported in the literature, there have been few epidemiologic studies. the purpose of this study was to review the epidemiology of patients with cutaneous m. marinum infection over a 3-year period at the national skin centre in singapore.200010849123
[sporotrichoid infection with mycobacterium marinum: successful therapy with oral tetracycline administration].atypical mycobacterial infections of the skin present a diagnostic and therapeutic challenge to dermatologists in many instances. we report on a patient who was diagnosed with atypical mycobacteriosis in its rarer, sporotrichoid form. possible differential diagnoses are discussed. efficient therapy using minocycline is demonstrated.200010875074
mycobacterium marinum hand infection: case reports and review of literature.three patients with mycobacterium marinum infection are described. key elements in the diagnosis of this infection are a high index of suspicion, taking a history with an emphasis on exposure to tropical fish or other potential sources of m. marinum infection, and tissue biopsy for culture and histology. the microbiologist should be informed about the suspicion of m. marinum infection so that appropriate cultures can be performed. as m. marinum does not grow under routine culture conditions, the ...200010878841
incubation period and sources of exposure for cutaneous mycobacterium marinum infection: case report and review of the literature.the diagnosis of cutaneous mycobacterium marinum infection is often delayed for months after presentation, perhaps because important clinical clues in the patient's history are frequently overlooked. knowledge of the incubation period allows the clinician to target questions about the patient's history. prompted by a case with a prolonged incubation period, we sought to determine more precisely the incubation period of m. marinum infection. the medline database for the period 1966-1996 was searc ...200010987702
[cutaneous nodular infection with hemopathy].a 36-year-old man with treated hairy cell leukemia developed a skin infection due to mycobacterium marinum. a spectrum of atypical cutaneous mycobacteriosis presentations with immunosuppression can be found. the recognition of the disease needs cutaneous biopsies for histopathology and its identification by specific laboratory methods to adapt treatment.200011015652
antibiotic susceptibility pattern of mycobacterium marinum.in vitro activities of 17 antibiotics against 53 clinical strains of mycobacterium marinum, an atypical mycobacterium responsible for cutaneous infections, were determined using the reference agar dilution method. rifampin and rifabutin were the most active drugs (mics at which 90% of the isolates tested were inhibited [mic(90)s], 0.5 and 0.6 microgram/ml, respectively). mics of minocycline (mic(90), 4 microgram/ml), doxycycline (mic(90), 16 microgram/ml), clarithromycin (mic(90), 4 microgram/ml ...200011036036
applications of gene fusions to green fluorescent protein and flow cytometry to the study of bacterial gene expression in host cells. 200011036634
skin disease and nontuberculous atypical mycobacteria. 200011044189
comparative genetic analysis of mycobacterium ulcerans and mycobacterium marinum reveals evidence of recent divergence.previous studies of the 16s rrna genes from mycobacterium ulcerans and mycobacterium marinum have suggested a very close genetic relationship between these species (99.6% identity). however, these organisms are phenotypically distinct and cause diseases with very different pathologies. to investigate this apparent paradox, we compared 3,306 nucleotides from the partial sequences of eight housekeeping and structural genes derived from 18 m. ulcerans strains and 22 m. marinum strains. this analysi ...200011053375
a case report of mycobacterium marinum infection of the hand.we report a case of mycobacterium marinum infection of the hand presenting initially as triggering of the digits. we like to highlight the unusual source of the infection and difficulty of diagnosis in this case as well as the various treatment modalities.200011063172
[aquariums and mycobacterioses].mycobacterium infections are increasingly reported in fish fanciers who keep an aquarium. inoculation occurs on abraded skin after an incubation period of 2 to 3 weeks. clinically papulo-nodules, ulcers or verrucous plaques develop. these may progress into sporotrichoid lesions or into deeper infections involving tendons and bone. cultural identification is obtained by biopsy specimen at low temperature (30-33 degrees c). faster identification may be performed by polymerase chain reaction. histo ...200011068476
susceptibility testing for mycobacteria.mycobacterial susceptibility testing is important for the management of patients with tuberculosis and those with disease caused by certain nontuberculous mycobacteria. to help standardize methods used in the clinical microbiology laboratory for testing susceptibility of mycobacteria, the national committee for clinical laboratory standards (nccls) recently updated nccls document m24-t (published in 1995), which is the tentative standard for antimycobacterial susceptibility testing of mycobacter ...200011073754
[rapidly growing mycobacteria in human pathology]. 200011075485
variable numbers of ttc repeats in mycobacterium leprae dna from leprosy patients and use in strain differentiation.strain differentiation of mycobacterium leprae would be of great value for epidemiological investigation to identify the infectious sources of leprosy, to understand transmission patterns, and to distinguish between relapse and reinfection. from the m. leprae genome sequence database, ttc dna repeats were identified. primer sets designed to amplify the region flanking ttc repeats revealed pcr products of different sizes, indicating that the number of repeats at each locus may be variable among m ...200011101592
[chronic paronychia and synovialitis of extensor tendons due to mycobacterium marinum. is diagnosis or treatment the problem?].most infections of the upper extremity are caused by staphylococcus or streptococcus and respond well to beta-lactam antibiotics. hand surgeons should be aware of the possible diagnosis of mycobacterium marinum infection: 90% of the lesions are found in the upper extremity. we present a case of a chronic, cutaneous lesion of the right middle finger with synovialitis of the extensor tendons observed in a 35-year-old woman. routine cultures from tissue of the infected finger led to the diagnosis o ...200011103693
cutaneous blastomycosis in new brunswick: case report.blastomycosis is a fungal infection of immunocompetent hosts. we present a case of cutaneous blastomycosis acquired in new brunswick, which provides evidence that this disease is endemic in atlantic canada. this case also demonstrates that the diagnosis of blastomycosis may be elusive. perseverance, a high index of clinical suspicion and close cooperation with the microbiology laboratory may be required to diagnose this uncommon condition.200011107468
topically acquired bacterial zoonoses from fish: a review.the main pathogens acquired topically from fish (through spine puncture or open wounds) are aeromonas hydrophila, edwardsiella tarda, erysipelothrix rhusiopathiae, mycobacterium marinum, streptococcus iniae, vibrio vulnificus and vibrio damsela. s. iniae has recently emerged as a public health hazard associated with aquaculture, and m. marinum often infects home aquarium hobbyists. with the expansion of aquaculture and popularity of recreational fishing in australia, medical practitioners can ex ...200011130351
[mycobacterium marinum nodular lymphangitis].mycobacterium marinum has been recognized for some forty years. it generally occurs after trauma in a patient who manipulates tropical fish living in an aquarium.200011147048
[clinical presentation and therapy of mycobacterium marinum infection as seen in 12 cases].mycobacterium marinum (m.m.) is the causative pathogen of skin infections that have been called "swimming pool granulomas". an increasing number of reports that deep structures are involved in these infections was the reason for studying the clinical presentation and response of the infection to different therapeutic regimens.200010650818
detection of a new mycobacterium species in wild striped bass in the chesapeake bay.investigation into recent declines in striped bass health in the chesapeake bay in maryland resulted in the isolation of a putative new species of mycobacterium. this isolate was obtained from fish showing skin ulcers and internal granulomas in various organs. the isolate was slow growing at 28 degrees c; was nonchromogenic; showed no activities of nitrate reduction, catalase activity, tween 80 hydrolysis, tellurite reduction, or arylsulfatase reduction; grew best at low salt concentrations; and ...200111158132
production of a species specific monoclonal antibody against a 56-kda mycobacterium marinum antigen which is potentially useful in culture identification.a monoclonal antibody (moab) designated 1h11 has been produced against a 56-kda antigen from mycobacterium marinum. this moab has been shown to be species specific by both enzyme-linked immunosorbent assay and western blot. the relationship of this 56 kda to other known mycobacterial antigens is currently under investigation. moab 1h11 recognised bacilli taken from cultures by both immunofluorescence and immunoperoxidase staining. this moab 1h11 may provide a simple method for rapid identificati ...200111172985
sporotrichoid lymphocutaneous infections: etiology, diagnosis and therapy.sporotrichoid lymphocutaneous infection is an uncommon syndrome that is often misdiagnosed and improperly treated. of the several hundred cases seen each year in the united states, the majority are caused by sporothrix schenckii, nocardia brasiliensis, mycobacterium marinum or leishmania brasiliensis. the "sporotrichoid" disease begins at a site of distal inoculation and leads to the development of nodular lymphangitis. systemic symptoms are characteristically absent. by recognizing the distinct ...200111201697
mycobacterium marinum hand infection. 200111207141
multicenter study of incidence of mycobacterium marinum in humans in spain.a retrospective survey was carried out on the epidemiological monitoring of the isolation incidence of mycobacterium marinum in 21 laboratories, in an attempt to gain an insight into the frequency of its isolation in spain. from 1991 to 1998, 39 cases (22 male and 17 female) of bacteriologically confirmed m. marinum infection were accumulated. the majority of the cases (35) were fish related. the clinical presentation usually suggested sporotrichosis, with the majority of skin eruptions appearin ...200111258515
mycobacterial infections in domestic and wild animals due to mycobacterium marinum, m. fortuitum, m. chelonae, m. porcinum, m. farcinogenes, m. smegmatis, m. scrofulaceum, m. xenopi, m. kansasii, m. simiae and m. genavense.the epidemiology and the natural distribution of mycobacterium marinum, m. fortuitum, m. chelonae, m. porcinum, m. farcinogenes, m. smegmatis, m. scrofulaceum, m. xenopi, m. kansasii, m. simiae and m. genavense are described. in addition to the bacteriological, biochemical and genetic characteristics, the authors review the pathology of these species, including the natural and experimental diseases and the accompanying lesions, diagnosis, antibiotic sensitivities and treatment of animal infectio ...200111288516
mycobacterium marinum infection in a lung transplant recipient.we report a case of mycobacterium marinum infection in a lung transplant recipient who presented with nodules on the hand and forearm following exposure to fish-tank water of a superficial hand burn. skin biopsy revealed granulomatous inflammation and fibrosis. tissue culture grew mycobacterium marinum. the patient underwent surgical excision of the lesions and treatment with ethambutol and azithromycin for 12 months and experienced complete resolution of the infection. transplant recipients who ...200111295588
a case of disseminated mycobacterium marinum infection following systemic steroid therapy.we describe the dissemination of mycobacterium marinum infection from the right middle finger to the whole extremity and two legs, with the involvement of tendons and joints, in a patient who was treated with a steroid when mycobacterium marinum infection was not suspected. the patient was successfully treated with a combined surgical and medical approach.200111303818
unusual and memorable. a tender swelling of the dorsum of a hand in a middle aged woman. 200111350842
sporotrichoid lymphangitis due to group a streptococcus.we describe what is, to our knowledge, the first case of sporotrichoid lymphangitis caused solely by group a streptococcus in an otherwise healthy patient. infection with pyogenic pathogens, such as streptococci and staphylococci, as well as with the most common causes of sporotrichoid lymphangitis (i.e., sporothrix schenkii, nocardia brasiliensis, mycobacterium marinum, and leishmania species) should be considered in differential diagnosis for some patients.200111360227
identification of mycobacterium marinum in sea-urchin granulomas.sea-urchin granuloma is a chronic granulomatous reaction arising after injury with sea-urchin spines. classified as an allergic foreign-body type of granuloma, it is believed to be a delayed-type reaction to an as yet unidentified antigen. in a clinicopathological study, 50 biopsy specimens from 35 patients diagnosed as having sea-urchin granuloma caused by paracentrotus lividus, we found different inflammatory patterns that in some cases suggested a mycobacterial infection.200111453918
the use of is2404 restriction fragment length polymorphisms suggests the diversity of mycobacterium ulcerans from different geographical areas.buruli ulcer, caused by mycobacterium ulcerans, has been reported in five continents: africa, asia, australia, and north and south america. in the present study, restriction fragment length polymorphism with the recently described m. ulcerans specific insertion sequence is2404 as a probe, was applied to mycobacterium shinshuense, mycobacterium marinum, and 14 clinical m. ulcerans isolates originating from six geographic areas: africa (n = 6), australia (n = 2), mexico (n = 1), south asia (n = 2) ...200111463115
antimicrobial susceptibility of mycobacterium marinum determined by e-test and agar dilution.mycobacterium marinum is recognized as a cutaneous pathogen requiring antibiotic treatment. we compared the e-test with a reference agar dilution method for susceptibility testing of m. marinum to amikacin, ciprofloxacin, clarithromycin, doxycycline, rifampicin, trimethoprim-sulfamethoxazole and ethambutol. mics obtained after 6 d showed agreement between the e-test and agar dilution within +/- 2 dilutions in 95% of all cases for amikacin, ciprofloxacin, doxycycline and rifampicin. inhibitory co ...200111525351
evaluation of pcr-restriction profile analysis and is2404 restriction fragment length polymorphism and amplified fragment length polymorphism fingerprinting for identification and typing of mycobacterium ulcerans and m. marinum.mycobacterium ulcerans and m. marinum are emerging necrotizing mycobacterial pathogens that reside in common reservoirs of infection and exhibit striking pathophysiological similarities. furthermore, the interspecific taxonomic relationship between the two species is not clear as a result of the very high phylogenetic relatedness (i.e., >99.8% 16s rrna sequence similarity), in contrast to only 25 to 47% dna relatedness. to help understand the genotypic affiliation between these two closely relat ...200111526162
production of selenomethionine-labelled proteins using simplified culture conditions and generally applicable host/vector systems.the amino acid analogue selenomethionine (semet) is shown to be efficiently incorporated into recombinant proteins expressed in escherichia coli grown in a simple minimal medium without the addition of synthetic amino acids. furthermore, satisfactory semet incorporation is obtained with a methionine-prototrophic strain transformed with commonly used vector systems. as examples, purified tryparedoxin 1 from crithidia fasciculata, alkylhydroperoxide reductase (ahpc) from mycobacterium marinum and ...200111601620
rapid identification of cutaneous infections by nontubercular mycobacteria by polymerase chain reaction-restriction analysis length polymorphism of the hsp65 gene.nontubercular mycobacteria (ntm) may cause cutaneous infections which are difficult to interpret due to the variability of the clinical manifestations. this study involved eight patients (four men and four women) with primary cutaneous infections caused by ntm; the skin lesions included dermo-hypodermal abscesses, suppurative granulomas, and papulonodules localized on the legs, arms, hands, and face. the general condition of the patients was relatively good and they were not immunosuppressed.200111703519
fish monocytes as a model for mycobacterial host-pathogen interactions.mycobacterium marinum, a relatively rapid-growing fish and human pathogen, has become an important model for the investigation of mycobacterial pathogenesis. m. marinum is closely related to the mycobacterium tuberculosis complex and causes a disease in fish and amphibians with pathology similar to tuberculosis. we have developed an in vitro model for the study of m. marinum virulence mechanisms using the carp monocytic cell line clc (carp leukocyte culture). we found that fish monocytes can dif ...200111705902
dynamic nature of host-pathogen interactions in mycobacterium marinum granulomas.mycobacterium marinum causes long-term subclinical granulomatous infection in immunocompetent leopard frogs (rana pipiens). these granulomas, organized collections of activated macrophages, share many morphological features with persistent human tuberculous infection. we examined organs of frogs with chronic m. marinum infection using transmission electron microscopy in conjunction with immunohistochemistry and acid phosphatase cytochemistry to better define the bacterium-host interplay during p ...200111705964
mycobacterium marinum dermatitis and panniculitis with chronic pleuritis in a captive white whale (delphinapterus leucas) with aortic rupture.a 16-year-old female white whale, delphinapterus leucas, died after nearly 18 months of chronic lymphopenia and pyogranulomatous dermatitis. necropsy revealed rupture of the aorta with hemorrhage into the cranial mediastinum and between fascial planes of the ventral neck musculature. multiple foci of ulcerative dermatitis and panniculitis were present across the thorax and abdomen and surrounded the genital folds. in addition, there was a chronic proliferative pleuritis with over 20 liters of hi ...200111724146
a unique mycobacterium species isolated from an epizootic of striped bass (morone saxatilis).we isolated a mycobacterium sp. resembling mycobacterium marinum and m. ulcerans from diseased striped bass (morone saxatilis) during an epizootic of mycobacteriosis in the chesapeake bay. this isolate may represent an undescribed mycobacterium species, based on phenotypic characteristics and comparative 16s rrna gene sequence.200111747708
mycobacterium marinum tenosynovitis in a patient with still's disease. 200111752518
case report--slowly enlarging mass of the dorsal right hand in a pregnant woman. 200111965202
[nontuberculous mycobacterial infections]. 200111977501
[lymphocutaneous syndrome due to mycobacterium marinum in a young aquarium enthusiast]. 200111844463
a promoter probe plasmid based on green fluorescent protein : a strategy for studying meningococcal gene expression.many bacterial genes are regulated in an environment-responsive fashion, and from the perspective of a pathogen, the host represents just another environment. many genes that contribute to virulence are differentially expressed in response to host environments that they encounter during colonization and invasion (1). recognition of this has led to the development of selection or reporter systems that utilize the increased activity of promoters during growth in vivo to identify genes that are sel ...200121337172
in vitro activity of 11 antimicrobial agents, including gatifloxacin and gar936, tested against clinical isolates of mycobacterium marinum.in vitro activities of 11 antimicrobial agents were determined using etest (ab biodisk, solna, sweden) against 37 clinical isolates of mycobacterium marinum. the most potent agent (mic(50,) 0.047 microg/ml) was trimethoprim/sulfamethoxazole, but only 91.9% would be categorized as susceptible. several agents had a complete spectrum of activity including gatifloxacin (mic(90,) 0.38 microg/ml), minocycline (mic(90,) 2 microg/ml), tetracycline (mic(90,) 2 microg/ml) and amikacin (mic(90,) 3 microg/m ...200211858912
complex pattern of mycobacterium marinum gene expression during long-term granulomatous infection.during latent infection of humans with mycobacterium tuberculosis, bacteria persist in the asymptomatic host within granulomas, organized collections of differentiated macrophages, and other immune cells. the mechanisms for persistence remain poorly understood, as is the metabolic and replicative state of the microbes within granulomas. we analyzed the gene expression profile of mycobacterium marinum, the cause of fish and amphibian tuberculosis, during its persistence in granulomas. we identifi ...200211891270
susceptibilities of mycobacterium marinum to gatifloxacin, gemifloxacin, levofloxacin, linezolid, moxifloxacin, telithromycin, and quinupristin-dalfopristin (synercid) compared to its susceptibilities to reference macrolides and quinolones.the susceptibility pattern of mycobacterium marinum was determined. quinupristin-dalfopristin and telithromycin were less active than clarithromycin. linezolid showed good antimicrobial activity at clinically achievable concentrations. gatifloxacin, levofloxacin, and moxifloxacin displayed activities similar to those of ciprofloxacin. gemifloxacin was less active. the etest method showed variable agreement with the reference method.200211897601
[skin nodules and ulcers of the limbs in a patient with rheumatoid arthritis].while being treated with corticosteroids and methotrexate for rheumatoid arthritis, a 63-year-old man developed livid nodules on his lower arms, hands and feet, as well as fever, necrotizing skin ulcers and rupture of a finger extensor tendon.200211935470
mycobacterium marinum infection in taiwan.mycobacterium marinum often causes skin infections, tenosynovitis, arthritis, and osteomyelitis, and occasionally results in severe disseminated infections in immunocompromised patients. in this study, the clinical features of 14 cases of m. marinum infection were retrospectively analyzed. one patient had septic arthritis, the other 13 had skin infections and/or tenosynovitis. it usually took 2 months or longer for a definite diagnosis to be made in these patients. three of the 14 patients were ...200211950119
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