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lactobacillus salivarius ren inhibits rat oral cancer induced by 4-nitroquioline 1-oxide.despite significant advances in cancer therapy, cancer-related mobility and mortality are still rising. alternative strategies such as cancer prevention thus become essential. probiotics represent an emerging option for cancer prevention, but studies are limited to colon cancers. the efficiency of probiotics in the prevention of other cancers and the correlative mechanism remains to be explored. a novel probiotics lactobacillus salivarius ren (l. salivarius ren) was isolated from centenarians at ...201323658366
lactobacillus salivarius: bacteriocin and probiotic activity.lactic acid bacteria (lab) antimicrobial peptides typically exhibit antibacterial activity against food-borne pathogens, as well as spoilage bacteria. therefore, they have attracted the greatest attention as tools for food biopreservation. in some countries lab are already extensively used as probiotics in food processing and preservation. lab derived bacteriocins have been utilized as oral, topical antibiotics or disinfectants. lactobacillus salivarius is a promising probiotic candidate commonl ...201324010610
antimicrobial potential for the combination of bovine lactoferrin or its hydrolysate with lactoferrin-resistant probiotics against foodborne pathogens.previous reports have shown that several probiotic strains can resist the antibacterial activity of bovine lactoferrin (blf), but the results are inconsistent. moreover, a portion of orally administered apo-blf is digested in vivo by pepsin to yield blf hydrolysate, which produces stronger antibacterial activity than that observed with apo-blf. however, whether blf hydrolysate affects the growth of probiotic strains is unclear. therefore, various probiotic strains in taiwan were collected and ev ...201323332852
potential probiotic lactic acid bacteria of human origin induce antiproliferation of colon cancer cells via synergic actions in adhesion to cancer cells and short-chain fatty acid bioproduction.the activities and modes of probiotic action of lactic acid bacteria isolated from infant feces were investigated for alternative application in the prevention and biotherapy of colon cancer. from a total of 81 isolates of gram-positive rod and cocci bacteria obtained from healthy infants, only 15 isolates had the probiotic criteria which included growth inhibition against eight food-borne pathogens, no blood hemolysis, and tolerance to gastrointestinal tract properties such as ph 2.5 and 0.3 % ...201323239414
isolation and characterization of bacteriocin-producing bacteria from the intestinal microbiota of elderly irish subjects.to isolate and characterize bacteriocins produced by predominant species of lactic acid bacteria from faeces of elderly subjects.201323181509
co-administration of bacillus subtilis rjgp16 and lactobacillus salivarius b1 strongly enhances the intestinal mucosal immunity of piglets.probiotics, including bacillus spp. and lactobacillus, are potential replacements for low dose in-feed antibiotics for pig. this study aimed to evaluate the effect of the co-administration of bacillus subtilis rjgp16 and lactobacillus salivarius b1 as potential probiotics to stimulate local immune responses. thirty two newborn piglets were divided into four groups and orally administrated with different combination of probiotics (none; rjgp16; b1; rjgp16 and b1) at the age of 0, 7 and 11 days. w ...201322901748
identification of lactobacillus strains with probiotic features from the bottlenose dolphin (tursiops truncatus).in order to develop complementary health management strategies for marine mammals, we used culture-based and culture-independent approaches to identify gastrointestinal lactobacilli of the common bottlenose dolphin, tursiops truncatus.201323855505
antibacterial efficacy of a human β-defensin-3 peptide on multispecies biofilms.the aggregation of mixed bacterial flora into sessile biofilms on root canal surfaces can be one of the causes of persistent apical periodontitis. the aim of this study was to evaluate the antibacterial efficacy of human β-defensin-3 (hbd3) peptide on multispecies biofilms by using confocal laser scanning microscopy.201324238461
effect of metalloporphyrins on red autofluorescence from oral bacteria.the aim of this study was to assess the red autofluorescence from bacterial species related to dental caries and periodontitis in the presence of different nutrients in the growth medium. bacteria were grown anaerobically on tryptic soy agar (tsa) supplemented with nutrients, including magnesium-porphyrins from spinach and iron-porphyrins from heme. the autofluorescence was then assessed at 405 nm excitation. on the tsa without additives, no autofluorescence was observed from any of the species ...201323659237
surface glycosaminoglycans mediate adherence between hela cells and lactobacillus salivarius lv72.the adhesion of lactobacilli to the vaginal surface is of paramount importance to develop their probiotic functions. for this reason, the role of hela cell surface proteoglycans in the attachment of lactobacillus salivarius lv72, a mutualistic strain of vaginal origin, was investigated.201324044741
bactofencin a, a new type of cationic bacteriocin with unusual immunity.bacteriocin production is an important probiotic trait of intestinal bacteria. in this study, we identify a new type of bacteriocin, bactofencin a, produced by a porcine intestinal isolate lactobacillus salivarius dpc6502, and assess its potency against pathogenic species including staphylococcus aureus and listeria monocytogenes. genome sequencing of the bacteriocin producer revealed bfna, which encodes the mature and highly basic (pi 10.59), 22-amino-acid defensin-like peptide. matrix-assisted ...201324169573
probiotic gut effect prevents the chronic psychological stress-induced brain activity abnormality in mice.a probiotic formulation (lactobacillus helveticus r0052 and bifidobacterium longum r0175 combination, probio'stick(®) ) displays anxiolytic-like activity and reduces apoptosis in the lymbic system in animal models of depression. based on the hypothesis that modulation of gut microbiota by this probiotic formulation has beneficial effects on brain activity in stress conditions, we report a set of probiotic-evoked physiological, cellular, and molecular events in the brain of probio'stick(®) pretre ...201424372793
unusual genome complexity in lactobacillus salivarius jcm1046.lactobacillus salivarius strains are increasingly being exploited for their probiotic properties in humans and animals. dissemination of antibiotic resistance genes among species with food or probiotic-association is undesirable and is often mediated by plasmids or integrative and conjugative elements. l. salivarius strains typically have multireplicon genomes including circular megaplasmids that encode strain-specific traits for intestinal survival and probiotic activity. linear plasmids are le ...201425201645
poultry farm hygiene: microbiological quality assessment of drinking water used in layer chickens managed under the battery cage and deep litter systems at three poultry farms in southwestern nigeria.water troughs from deep litter and caged chicken water troughs (drinkers) fixed to each of the different 3-tier cages containing layer chickens in farms a, b and c were subjected to a 7-day study which involved the monitoring of poultry farm hygiene. drinkers were washed before filling with water on day 1. for days 3, 5 and 7 water was served without prior washing. the occurrence and characterization of the bacteria isolates were investigated and data obtained were analyzed and compared. for the ...201424783781
screening of lactobacillus strains of domestic goose origin against bacterial poultry pathogens for use as probiotics.lactobacilli are natural inhabitants of human and animal mucous membranes, including the avian gastrointestinal tract. recently, increasing attention has been given to their probiotic, health-promoting capacities, among which their antagonistic potential against pathogens plays a key role. a study was conducted to evaluate probiotic properties of lactobacillus strains isolated from feces or cloacae of domestic geese. among the 104 examined isolates, previously identified to the species level by ...201425104766
lactobacillus salivarius and l. gasseri down-regulate aggregatibacter actinomycetemcomitans exotoxins expression.beneficial microbes, such as lactobacilli establish a symbiosis with the host and confer health-associated effects, by limiting the growth of indigenous pathogens and challenging microbes introduced by altered foods. nevertheless, there is scarce information on the effects of beneficial microbes on the virulence properties of bacterial species associated with oral diseases, such as periodontitis. aggregatibacter actinomycetemcomitans is a gram-negative species highly implicated in the etiology o ...201424860281
effect of bacterial cell-free supernatants on infectivity of norovirus surrogates.bacterial metabolic products were evaluated for inhibitory effects on viral propagation in cell culture. cell-free supernatants (cfs) were prepared from growth of enterococcus faecalis atcc 19433, pseudomonas fluorescens atcc 13525, escherichia coli 08, staphylococcus epidermidis atcc 12228, bacillus subtilis 168, bacillus coagulans 185a, b. coagulans 7050, clostridium sporogenes pa3679, and a commercial probiotic mixture of lactobacillus acidophilus, lactobacillus rhamnosus, bifidobacterium bif ...201424406013
in vitro and in vivo effects of beneficial vaginal lactobacilli on pathogens responsible for urogenital tract infections.the aim of this work was to evaluate the effects of beneficial human vaginal lactobacilli (lb) on urogenital pathogens through in vitro and in vivo experiments. co-aggregative and antimicrobial properties between five vaginal lb strains and urogenital pathogens or potential pathogens (streptococcus agalactiae, staphylococcus aureus and candida albicans strains) were assayed. also, associative cultures of lb strains and s. agalactiae were performed and bacterial growth, ph, lactic acid and hydrog ...201424523160
effect of intestinal colonisation by two lactobacillus strains on the immune response of gnotobiotic mice.the effect of intestinal colonisation on the immune system was investigated in germ-free mice monoassociated with lactobacillus strains isolated from calf faeces. single doses of lactobacillus acidophilus l36 or lactobacillus salivarius l38 were administered to germ-free mice by intragastric gavage. ten days later, the mice were euthanised. gene expression levels of interleukin 5 (il-5), il-6, il-10, il-12b, il-17a, gamma interferon (ifn-γ), transforming growth factor beta 1 (tgf-β1), and tumour ...201424939801
probiotics in the prevention of eczema: a randomised controlled trial.to evaluate a multistrain, high-dose probiotic in the prevention of eczema.201424947281
in vitro evaluation of the safety and probiotic properties of lactobacilli isolated from chicken and calves.a total of 73 chicken and calves isolates were diagnosed using matrix-assisted laser desorption ionization-time-of flight mass spectrometry (maldi-tof ms). after a preliminary subtractive screening based on the high acid tolerance at ph 2.5 and bile resistance at 0.3% oxgall, twenty isolates belonging to the species lactobacillus salivarius, lactobacillus agilis, lactobacillus reuteri, lactobacillus murinus and lactobacillus amylovorus were in vitro screened for the safety assessment and probiot ...201424291759
assessment of probiotics supplementation via feed or water on the growth performance, intestinal morphology and microflora of chickens after experimental infection with eimeria acervulina, eimeria maxima and eimeria tenella.in this study, the effect of probiotic supplementation via drinking water or feed on the performance of broiler chickens experimentally infected with sporulated oocysts of eimeria acervulina (5 × 10(4)), eimeria maxima and eimeria tenella (2 × 10(4) each one) at 14 days of age was evaluated. two hundred and forty 1-day-old ross 308 male chicks were separated into eight equal groups with three replicates. two of the groups, one infected with mixed eimeria oocysts and the other not, were given a b ...201424601749
effect of bile salt hydrolase inhibitors on a bile salt hydrolase from lactobacillus acidophilus.bile salt hydrolase (bsh), a widely distributed function of the gut microbiota, has a profound impact on host lipid metabolism and energy harvest. recent studies suggest that bsh inhibitors are promising alternatives to antibiotic growth promoters (agp) for enhanced animal growth performance and food safety. using a high-purity bsh from lactobacillus salivarius strain, we have identified a panel of bsh inhibitors. however, it is still unknown if these inhibitors also effectively inhibit the func ...201425526498
[substrate specificities of bile salt hydrolase 1 and its mutants from lactobacillus salivarius].in order to analyze the correlation between critical residues in the catalytic centre of bsh and the enzyme substrate specificity, seven mutants of lactobacillus salivarius bile salt hydrolase (bsh1) were constructed by using the escherichia coli pet-20b(+) gene expression system, rational design and site-directed mutagenesis. these bsh1 mutants exhibited different hydrolytic activities against various conjugated bile salts through substrate specificities comparison. among the residues being tes ...201425007580
enhanced expression of codon optimized mycobacterium avium subsp. paratuberculosis antigens in lactobacillus salivarius.it is well documented that open reading frames containing high gc content show poor expression in a+t rich hosts. specifically, g+c-rich codon usage is a limiting factor in heterologous expression of mycobacterium avium subsp. paratuberculosis (map) proteins using lactobacillus salivarius. however, re-engineering opening reading frames through synonymous substitutions can offset codon bias and greatly enhance map protein production in this host. in this report, we demonstrate that codon-usage ma ...201425237653
effects of lactobacillus salivarius-containing tablets on caries risk factors: a randomized open-label clinical trial.to evaluate the effects of the lactic acid bacterium lactobacillus salivarius on caries risk factors.201425178882
development of a potential probiotic fresh cheese using two lactobacillus salivarius strains isolated from human milk.cheeses have been proposed as a good alternative to other fermented milk products for the delivery of probiotic bacteria to the consumer. the objective of this study was to assess the survival of two lactobacillus salivarius strains (cect5713 and ps2) isolated from human milk during production and storage of fresh cheese for 28 days at 4°c. the effect of such strains on the volatile compounds profile, texture, and other sensorial properties, including an overall consumer acceptance, was also inv ...201424971351
aggregation factor as an inhibitor of bacterial binding to gut mucosa.modern research in the area of probiotics is largely devoted to discovering factors that promote the adherence of probiotic candidates to host mucosal surfaces. the aim of the present study was to test the role of aggregation factor (aggl) and mucin-binding protein (mbpl) from lactococcus sp. in adhesion to gastrointestinal mucosa. in vitro, ex vivo, and in vivo experiments in rats were used to assess the adhesive potential of these two proteins expressed in heterologous host lactobacillus saliv ...201424823989
regulation of host weight gain and lipid metabolism by bacterial bile acid modification in the gut.alterations in the gastrointestinal microbiota have been implicated in obesity in mice and humans, but the key microbial functions influencing host energy metabolism and adiposity remain to be determined. despite an increased understanding of the genetic content of the gastrointestinal microbiome, functional analyses of common microbial gene sets are required. we established a controlled expression system for the parallel functional analysis of microbial alleles in the murine gut. using this app ...201424799697
lactobacillus salivarius ren prevent the early colorectal carcinogenesis in 1, 2-dimethylhydrazine-induced rat model.the objective of this study was to investigate the impact of lactobacillus salivarius ren (ls) on modulating colonic micro flora structure and influencing host colonic health in a rat model with colorectal precancerous lesions.201424754742
covalent immobilization of hlf1-11 peptide on a titanium surface reduces bacterial adhesion and biofilm formation.bacterial infection represents a major cause of implant failure in dentistry. a common approach to overcoming this issue and treating peri-implant infection consists in the use of antibiotics. however, the rise of multidrug-resistant bacteria poses serious concerns to this strategy. a promising alternative is the use of antimicrobial peptides due to their broad-spectrum activity against bacteria and reduced bacterial resistance responses. the aim of the present study was to determine the in vitr ...201424704699
draft genome sequence of a novel lactobacillus salivarius strain isolated from piglet.lactobacillus salivarius is part of the vertebrate indigenous microbiota of the gastrointestinal tract, oral cavity, and milk. the properties associated with some l. salivarius strains have led to their use as probiotics. here we describe the draft genome of the pig isolate l. salivarius cp400, providing insights into host-niche specialization.201424526652
discovery of bile salt hydrolase inhibitors using an efficient high-throughput screening system.the global trend of restricting the use of antibiotic growth promoters (agp) in animal production necessitates the need to develop valid alternatives to maintain productivity and sustainability of food animals. previous studies suggest inhibition of bile salt hydrolase (bsh), an intestinal bacteria-produced enzyme that exerts negative impact on host fat digestion and utilization, is a promising approach to promote animal growth performance. to achieve the long term goal of developing novel alter ...201424454844
treatment of atopic dermatitis eczema with a high concentration of lactobacillus salivarius ls01 associated with an innovative gelling complex: a pilot study on adults.to evaluate the efficacy of a highly concentrated lactobacillus salivarius preparation containing a gelling complex formed by streptococcus thermophilus st10 and tara gum in the treatment of atopic dermatitis (ad).201425291127
preliminary results on clinical effects of probiotic lactobacillus salivarius ls01 in children affected by atopic dermatitis.the goal of this study was to evaluate the clinical efficacy of an intake of lactobacillus salivarius ls01 (dsm 22775) for the treatment of atopic dermatitis (ad) in children.201425291124
lactobacillus salivarius wb21-containing tablets for the treatment of oral malodor: a double-blind, randomized, placebo-controlled crossover trial--reply to letter. 201425241000
lactobacillus salivarius wb21 tablets for the treatment of oral malodor: a commentary. 201425240998
urinary metabolomic fingerprinting after consumption of a probiotic strain in women with mastitis.infectious mastitis is a common condition among lactating women, with staphylococci and streptococci being the main aetiological agents. in this context, some lactobacilli strains isolated from breast milk appear to be particularly effective for treating mastitis and, therefore, constitute an attractive alternative to antibiotherapy. a (1)h nmr-based metabolomic approach was applied to detect metabolomic differences after consuming a probiotic strain (lactobacillus salivarius ps2) in women with ...201424880136
converging effects of a bifidobacterium and lactobacillus probiotic strain on mouse intestinal physiology.evidence has grown to support the efficacy of probiotics in the management of gastrointestinal disorders, many of which are associated with dysregulated fluid and electrolyte transport. a growing body of evidence now suggests that the host microbiota and probiotics can influence intestinal ion transport and that these effects often occur in a strain-dependent manner. in this study, we sought to investigate the effects of two therapeutically relevant organisms, bifidobacterium infantis 35,624 and ...201424852567
protection of dried probiotic bacteria from bile using bile adsorbent resins.enteric coated oral tablets or capsules can deliver dried live cells directly into the intestine. previously, we found that a live attenuated bacterial vaccine acquired sensitivity to intestinal bile when dried, raising the possibility that although gastric acid can be bypassed, significant loss of viability might occur on release from an enteric coated oral formulations. here we demonstrate that some food-grade lyophilised preparations of lactobacillus casei and lactobacillus salivarius also sh ...201424080386
identification of lactobacillus strains of goose origin using maldi-tof mass spectrometry and 16s-23s rdna intergenic spacer pcr analysis.the objective of our study was to identify lactobacillus sp. strains of goose origin using maldi-tof mass spectrometry, its-pcr and its-pcr/rflp. all three techniques proved to be valuable tools for identification of avian lactobacilli and produced comparable classification results. lactobacillus strains were isolated from 100% of geese aged 3 weeks to 4 years, but from only 25% of chicks aged 1-10 days. among the 104 strains isolated, we distinguished 14 lactobacillus species. the dominant spec ...201424607713
characterization of some potentially probiotic lactobacillus strains isolated from iranian native chickens.the objective of the present study was to isolate, identify and characterize new lab strains with high probiotic potentials from iranian (isfahan) indigenous chickens. from 90 isolated labs, 11 isolates had high growth rate under different stress conditions, including acid (ph 2.5), bile (0.5% oxgall), salt (6-15%) and temperatures 15 and 45°c, and their aggregation time was less than 120 min. based on the molecular identification using 16s rdna sequencing and phylogenetic analysis, the isolates ...201425742971
lactobacillus salivarius wb21--containing tablets for the treatment of oral malodor: a double-blind, randomized, placebo-controlled crossover trial.this study evaluated the effect of probiotic intervention using lactobacilli on oral malodor.201424556493
in vitro evaluation of the probiotic and functional potential of lactobacillus strains isolated from fermented food and human intestine.this study aims to evaluate the functional and probiotic characteristics of eight indigenous lactobacillus strains in vitro. the selected lactobacilli include strains of lactobacillus casei subsp. casei, lactobacillus salivarius subsp. salicinius, lactobacillus fermentum, lactobacillus plantarum, lactobacillus delbrueckii subsp. lactis, lactobacillus delbrueckii subsp. bulgaricus, and lactobacillus rhamnosus. all strains tolerated both ph 2 for 3 h and 1% bile salt for 24 h. the strains cicc 231 ...201425046742
screening of indigenous oxalate degrading lactic acid bacteria from human faeces and south indian fermented foods: assessment of probiotic potential.lactic acid bacteria (lab) have the potential to degrade intestinal oxalate and this is increasingly being studied as a promising probiotic solution to manage kidney stone disease. in this study, oxalate degrading lab were isolated from human faeces and south indian fermented foods, subsequently assessed for potential probiotic property in vitro and in vivo. based on preliminary characteristics, 251 out of 673 bacterial isolates were identified as lab. a total of 17 strains were found to degrade ...201424723820
differential effects of lactobacilli on activation and maturation of mouse dendritic cells.lactic acid bacteria (lab) are of interest because of their potential to modulate immune responses. the effects of lab range from regulation to stimulation of the immune system. a series of studies were performed in vitro to study the effects of six lactic acid bacteria (lab), lactobacillus helveticus lh-2, lactobacillus acidophilus la-5, la-115, la-116 and la-14, and lactobacillus salivarius, on maturation and activation of mouse dendritic cells. production of tumour necrosis factor (tnf)-?, in ...201424913839
administration of lactobacillus salivarius li01 or pediococcus pentosaceus li05 improves acute liver injury induced by d-galactosamine in rats.this work investigated the effect of the intragastric administration of five lactic acid bacteria from healthy people on acute liver failure in rats. sprague-dawley rats were given intragastric supplements of lactobacillus salivarius li01, lactobacillus salivarius li02, lactobacillus paracasei li03, lactobacillus plantarum li04, or pediococcus pentosaceus li05 for 8 days. acute liver injury was induced on the eighth day by intraperitoneal injection of 1.1 g/kg body weight d-galactosamine (d-galn ...201424639205
cost-effective endo-mannanase from bacillus sp. cfr1601 and its application in generation of oligosaccharides from guar gum and as detergent additive.the indigenous bacteria bacillus sp. cfr1601 produced significant levels of endo-mannanase when grown on agro-wastes, namely, green gram husk and sunflower oil cake (25.6 iu/ml), used as sole carbon and nitrogen sources, respectively. under immobilized cell system, synthetic supports (polyurethane foam, scotch brite, polyester; up to 33.2 iu/ml) were found marginally superior as compared to natural supports (cotton and silk; up to 28.2 iu/ml) for endo-mannanase production. cooperative interactio ...201424320239
evaluation of immunomodulatory activity of two potential probiotic lactobacillus strains by in vivo tests.here we evaluate the immunomodulatory function of two potential probiotic strains, lactobacillus salivarius cicc 23174 and lactobacillus plantarum cgmcc 1.557. mice were fed with each lactobacillus strain at different doses for several consecutive days. the effects of the two probiotic strains on immune organs, immune cells and immune molecules were investigated on days 10 and 20. both lactobacillus strains increased the spleen index, improved the spleen lymphocyte transformation rate, enhanced ...201526143437
inhibitory effect of lactobacillus salivarius on streptococcus mutans biofilm formation.dental caries arises from an imbalance of metabolic activities in dental biofilms developed primarily by streptococcus mutans. this study was conducted to isolate potential oral probiotics with antagonistic activities against s. mutans biofilm formation from lactobacillus salivarius, frequently found in human saliva. we analysed 64 l. salivarius strains and found that two, k35 and k43, significantly inhibited s. mutans biofilm formation with inhibitory activities more pronounced than those of la ...201524961744
design of novel urogenital pharmabiotic formulations containing lactobacilli, salivaricin crl 1328 and non-microbial compounds with different functionalities.the administration of pharmabiotics is a promising alternative to antimicrobial drugs for the treatment and/or prevention of female urogenital infections.201524827975
antifouling coatings for dental implants: polyethylene glycol-like coatings on titanium by plasma polymerization.titanium dental implants are commonly used for the replacement of lost teeth, but they present a considerable number of failures due to the infection on surrounding tissues. the aim of this paper is the development of a polyethylene glycol-like (peg-like) coating on the titanium surface by plasma polymerization to obtain a novel improved surface with suitable low bacterial adhesion and adequate cell response. surface analysis data of these coatings are presented, in particular, water contact ang ...201525766480
impact of probiotics in women with gestational diabetes mellitus on metabolic health: a randomized controlled trial.probiotics are live microorganisms that may confer health benefits on the host. recent trials of probiotic use among healthy pregnant women demonstrate potential for improved glycemic control. the aim of this study was to investigate the effects of a probiotic capsule intervention on maternal metabolic parameters and pregnancy outcome among women with gestational diabetes.201525687568
effect of lactobacillus salivarius on th1/th2 cytokines and the number of spleen cd4⁺ cd25⁺ foxp3⁺ treg in asthma balb/c mouse.bronchial asthma is a chronic airway inflammatory disease that involves t lymphocytes.201526339333
impact of exopolysaccharide production on functional properties of some lactobacillus salivarius strains.the aim of this work was to characterize functional properties of lactobacillus salivarius strains isolated from chicken feces. detection of genes responsible for exopolysaccharide (eps) production revealed that all strains harbored a dextransucrase gene, but p-gtf gene was only detected in strain e4. analysis of eps production levels showed significant alterations among strains tested. biofilm formation was found to be medium composition dependant, and there was a negative correlation with biof ...201526267164
in vitro and in vivo screening of native lactic acid bacteria toward their selection as a probiotic in broiler chickens.among 360 isolates from the gastrointestinal tract (git) of broilers, eleven isolates which showed in vitro probiotic properties were identified and selected for further tests. after the in vitro screening, three strains were chosen for the in vivo study of persistence of fresh cultures and then one strain was selected for the in vivo study of persistence of lyophilized culture. lyophilized lactobacillus salivarius dspv 001p was capable of persisting in broilers during a complete rearing, even 2 ...201526267089
the oral and gut microbiomes are perturbed in rheumatoid arthritis and partly normalized after treatment.we carried out metagenomic shotgun sequencing and a metagenome-wide association study (mgwas) of fecal, dental and salivary samples from a cohort of individuals with rheumatoid arthritis (ra) and healthy controls. concordance was observed between the gut and oral microbiomes, suggesting overlap in the abundance and function of species at different body sites. dysbiosis was detected in the gut and oral microbiomes of ra patients, but it was partially resolved after ra treatment. alterations in th ...201526214836
heterophil phagocytic activity stimulated by lactobacillus salivarius l61 and l55 supplementation in broilers with salmonella infection.newborn chicks are susceptible to salmonella enterica serovar enteritidis (se). the objective of this study was to evaluate the effect of lactobacillus probiotic isolated from chicken feces on heterophil phagocytosis in broiler chicks. a total of 150 newborn broiler chicks were divided into 5 groups (30 chicks per group) as follows: group 1 (normal control), given feed and water only, group 2 (positive control) given feed, water and se infection, group 3 (l61 treated) given feed, water, se infec ...201526580288
impact of environmental factors on bacteriocin promoter activity in gut-derived lactobacillus salivarius.bacteriocin production is regarded as a desirable probiotic trait that aids in colonization and persistence in the gastrointestinal tract (git). strains of lactobacillus salivarius, a species associated with the git, are regarded as promising probiotic candidates and have a number of associated bacteriocins documented to date. these include multiple class iib bacteriocins (salivaricin t, salivaricin p, and abp-118) and the class iid bacteriocin bactofencin a, which show activity against medicall ...201526341205
detection and genomic characterization of motility in lactobacillus curvatus: confirmation of motility in a species outside the lactobacillus salivarius clade.lactobacillus is the largest genus within the lactic acid bacteria (lab), with almost 180 species currently identified. motility has been reported for at least 13 lactobacillus species, all belonging to the lactobacillus salivarius clade. motility in lactobacilli is poorly characterized. it probably confers competitive advantages, such as superior nutrient acquisition and niche colonization, but it could also play an important role in innate immune system activation through flagellin–toll-like r ...201525501479
lactobacillus and lactobacillus cell-free culture supernatants modulate chicken macrophage activities.lactobacilli are commensal microbes that reside in the intestines of several species, including chickens. structural constituents of lactobacilli are able to stimulate the host immune system. macrophages are crucial players in both innate and adaptive immune systems. here, we investigated the effects of lactobacillus acidophilus, lactobacillus reuteri, lactobacillus salivarius and their cell-free culture supernatants on the pro-inflammatory gene expression profile, nitric oxide (no) production a ...201526679813
characterization of the effects of three lactobacillus species on the function of chicken macrophages.lactobacillus acidophilus, lactobacillus reuteri and lactobacillus salivarius can influence the adaptive immune responses in chickens but vary in their ability to do so. the present study attempted to identify how these three bacteria alter the innate immune system. a chicken macrophage cell line, mq-ncsu, was co-cultured with the three live lactobacillus species, alone or in combination, grown at different temperatures for various durations of time. late exponential growth phase bacteria were m ...201525847283
clinical characteristics of bacteraemia caused by lactobacillus spp. and antimicrobial susceptibilities of the isolates at a medical centre in taiwan, 2000-2014.the clinical characteristics of 89 patients with lactobacillus bacteraemia treated at a university-affiliated hospital in northern taiwan during 2000-2014 were retrospectively evaluated. lactobacillus spp. were identified by 16s rrna sequencing analysis and matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (maldi-tof/ms). antimicrobial susceptibilities of the isolates were determined by broth microdilution. the most commonly isolated species was lactobacillus salivariu ...201526298673
identification and characterization of intestinal lactobacilli strains capable of degrading immunotoxic peptides present in gluten.identify and characterize bacteria from the proximal gastrointestinal tract of pigs capable of degrading immunogenic gluten peptides.201525376327
effect of probiotic lactobacillus salivarius ubl s22 and prebiotic fructo-oligosaccharide on serum lipids, inflammatory markers, insulin sensitivity, and gut bacteria in healthy young volunteers: a randomized controlled single-blind pilot study.this study investigated the effect of 6-week supplementation of a probiotic strain lactobacillus salivarius ubl s22 with or without prebiotic fructo-oligosaccharide (fos) on serum lipid profiles, immune responses, insulin sensitivity, and gut lactobacilli in 45 healthy young individuals. the patients were divided into 3 groups (15/group), that is, placebo, probiotic, and synbiotic. after 6 weeks, a significant reduction (p < .05) in total cholesterol, low-density lipoprotein (ldl) cholesterol, a ...201525331262
subspecies diversity in bacteriocin production by intestinal lactobacillus salivarius strains.a recent comparative genomic hybridization study in our laboratory revealed considerable plasticity within the bacteriocin locus of gastrointestinal strains of lactobacillus salivarius. most notably, these analyses led to the identification of two novel unmodified bacteriocins, salivaricin l and salivaricin t, produced by the neonatal isolate l. salivarius dpc6488 with immunity, regulatory and export systems analogous to those of abp118, a two-component bacteriocin produced by the well character ...201522892690
complete genome sequence of lactobacillus salivarius ren, a probiotic strain with anti-tumor activity.lactobacillus salivarius ren (lsr) (cgmcc no. 3606) is a probiotic strain that was isolated from the feces of a healthy centenarian living in bama, guangxi, china. previous studies have shown that this strain decreases 4-nitroquinoline 1-oxide (4-nqo)-induced genotoxicity in vitro. it also suppresses 4-nqo-induced oral carcinogenesis and 1,2-dimethylhydrazine (dmh)-induced colorectal carcinogenesis, and therefore may be used as an adjuvant therapeutic agent for cancer. here, we report the comple ...201526133929
functional role of oppa encoding an oligopeptide-binding protein from lactobacillus salivarius ren in bile tolerance.lactobacillus salivarius is a member of the indigenous microbiota of the human gastrointestinal tract (git), and some l. salivarius strains are considered as probiotics. bile tolerance is a crucial property for probiotic bacteria to survive the transit through the git and exert their beneficial effects. in this work, the functional role of oppa encoding an oligopeptide transporter substrate-binding protein from l. salivarius ren in bile salt tolerance was investigated. in silico analysis reveale ...201525998246
improvement of lysm-mediated surface display of designed ankyrin repeat proteins (darpins) in recombinant and nonrecombinant strains of lactococcus lactis and lactobacillus species.safety and probiotic properties make lactic acid bacteria (lab) attractive hosts for surface display of heterologous proteins. protein display on nonrecombinant microorganisms is preferred for therapeutic and food applications due to regulatory requirements. we displayed two designed ankyrin repeat proteins (darpins), each possessing affinity for the fc region of human igg, on the surface of lactococcus lactis by fusing them to the usp45 secretion signal and to the peptidoglycan-binding c termin ...201525576617
antibacterial properties of hlf1-11 peptide onto titanium surfaces: a comparison study between silanization and surface initiated polymerization.dental implant failure can be associated with infections that develop into peri-implantitis. in order to reduce biofilm formation, several strategies focusing on the use of antimicrobial peptides (amps) have been studied. to covalently immobilize these molecules onto metallic substrates, several techniques have been developed, including silanization and polymer brush prepared by surface-initiated atom transfer radical polymerization (atrp), with varied peptide binding yield and antibacterial per ...201525545728
effect of ph and dilution rate on specific production rate of extra cellular metabolites by lactobacillus salivarius uco_979c in continuous culture.the effect of ph and dilution rate on the production of extracellular metabolites of lactobacillus salivarius uco_979 was studied. the experiments were carried out in continuous mode, with chemically defined culture medium at a temperature of 37 °c, 200 rpm agitation and synthetic air flow of 100 ml/min. ethanol, acetic acid, formic acid, lactic acid and glucose were quantified through hplc, while exopolysaccharide (eps) was extracted with ethanol and quantified through the dubois method. the re ...201525805342
silver deposition on titanium surface by electrochemical anodizing process reduces bacterial adhesion of streptococcus sanguinis and lactobacillus salivarius.the aim of this study was to determine the antibacterial properties of silver-doped titanium surfaces prepared with a novel electrochemical anodizing process.201524890701
lactic acid bacteria as a surface display platform for campylobacter jejuni antigens.food poisoning and diarrheal diseases continue to pose serious health care and socioeconomic problems worldwide. campylobacter spp. is a very widespread cause of gastroenteritis. over the past decade there has been increasing interest in the use of lactic acid bacteria (lab) as mucosal delivery vehicles. they represent an attractive opportunity for vaccination in addition to vaccination with attenuated bacterial pathogens.201525662187
a randomized controlled trial to test the effect of multispecies probiotics on cognitive reactivity to sad mood.recent insights into the role of the human microbiota in cognitive and affective functioning have led to the hypothesis that probiotic supplementation may act as an adjuvant strategy to ameliorate or prevent depression.201525862297
immunomodulatory effects of lactobacillus salivarius ls01 and bifidobacterium breve br03, alone and in combination, on peripheral blood mononuclear cells of allergic asthmatics.the aim of this study was to evaluate probiotic characteristics of lactobacillus salivarius ls01 and bifidobacterium breve br03 alone and in combination and their immunomodulatory activity in asthmatic subjects. subjects affected by allergic asthma were recruited. initially, ls01 and br03 were analyzed for their growth compatibility by a broth compatibility assay. to study the antimicrobial activity of probiotic strains, an agar diffusion assay was performed. finally, cytokine production by peri ...201525749784
administration of bifidobacterium breve ps12929 and lactobacillus salivarius ps12934, two strains isolated from human milk, to very low and extremely low birth weight preterm infants: a pilot study.the preterm infant gut has been described as immature and colonized by an aberrant microbiota. therefore, the use of probiotics is an attractive practice in hospitals to try to reduce morbidity and mortality in this population. the objective of this pilot study was to elucidate if administration of two probiotic strains isolated from human milk to preterm infants led to their presence in feces. in addition, the evolution of a wide spectrum of immunological compounds, including the inflammatory b ...201525759843
strains of enterococcus faecalis differ in their ability to coexist in biofilms with other root canal bacteria.to investigate the relationship between protease production and the ability of enterococcus faecalis strains to coexist in biofilms with other bacteria commonly recovered from infected root canals.201526172346
effects of lactobacillus salivarius ren on cancer prevention and intestinal microbiota in 1, 2-dimethylhydrazine-induced rat model.probiotics have been suggested as a prophylactic measure in colon cancer. the aim of this study was to investigate the impact of lactobacillus salivarius ren (ren) in modulating colonic microbiota structure and colon cancer incidence in a rat model after injection with 1,2-dimethyl hydrazine (dmh). the results indicated that oral administration of ren could effectively suppress dmh-induced colonic carcinogenesis. a significant decrease in cancer incidence (87.5% to 25%) was detected in rats fed ...201526025172
microbial inhibition of oral epithelial wound recovery: potential role for quorum sensing molecules?awareness of the impact of microbiota in both health and disease is growing. using a new in vitro oral mucosa co-culture model, we recently showed a clear inhibition of epithelial wound healing in the presence of an oral microbial community. in this paper, we have used the same model in combination with specific oral microbial species to obtain a better insight into the role of the oral microbiota in wound healing. monocultures of klebsiella oxytoca and lactobacillus salivarius significantly inh ...201525995981
high-resolution structures of lactobacillus salivarius transketolase in the presence and absence of thiamine pyrophosphate.probiotic bacterial strains have been shown to enhance the health of the host through a range of mechanisms including colonization, resistance against pathogens, secretion of antimicrobial compounds and modulation of the activity of the innate immune system. lactobacillus salivarius ucc118 is a well characterized probiotic strain which survives intestinal transit and has many desirable host-interaction properties. probiotic bacteria display a wide range of catabolic activities, which determine t ...201526457526
characterization of lactobacillus salivarius alanine racemase: short-chain carboxylate-activation and the role of a131.many strains of lactic acid bacteria produce high concentrations of d-amino acids. among them, lactobacillus salivarius ucc 118 produces d-alanine at a relative concentration much greater than 50 % of the total d, l-alanine (100d/d, l-alanine). we characterized the l. salivarius alanine racemase (alr) likely responsible for this d-alanine production and found that the enzyme was activated by carboxylates, which is an unique characteristic among alrs. in addition, alignment of the amino acid sequ ...201526543773
heterologous surface display on lactic acid bacteria: non-gmo alternative?lactic acid bacteria (lab) are food-grade hosts for surface display with potential applications in food and therapy. alternative approaches to surface display on lab would avoid the use of recombinant dna technology and genetically-modified organism (gmo)-related regulatory requirements. non-covalent surface display of proteins can be achieved by fusing them to various cell-wall binding domains, of which the lysine motif domain (lysm) is particularly well studied. fusion proteins have been isola ...201525880164
the comparative evaluation of the antimicrobial effect of propolis with chlorhexidine against oral pathogens: an in vitro study.this study aimed to compare the antimicrobial effectiveness of ethanolic extract of propolis (eep) to chlorhexidine gluconate (chx) on planktonic streptococcus mutans, streptococcus sobrinus, lactobacillus acidophilus, lactobacillus salivarius subsp. salivarius, aggregatibacter actinomycetemcomitans, prevotella intermedia, porphyromonas gingivalis, staphylococcus aureus, enterococcus faecalis, actinomyces israelii, candida albicans, and their single-species biofilms by agar dilution and broth mi ...201626949701
antibacterial effects of n-acetylcysteine against endodontic pathogens.the success of endodontic treatment depends on the eradication of microorganisms from the root canal system and the prevention of reinfection. the purpose of this investigation was to evaluate the antibacterial and antibiofilm efficacy of n-acetylcysteine (nac), an antioxidant mucolytic agent, as an intracanal medicament against selected endodontic pathogens. minimum inhibitory concentrations (mics) of nac for actinomyces naeslundii, lactobacillus salivarius, streptococcus mutans, and enterococc ...201627033208
characterization of antimicrobial substance from lactobacillus salivarius kl-d4 and its application as biopreservative for creamy filling.lactobacillus salivarius kl-d4 isolated from duck intestine produced bacteriocin which was stable at high temperature and a wide ph range of 3-10. its cell free supernatant at ph 5.5 exhibited wide inhibitory spectrum against both g+ and g- bacteria. the highest bacteriocin production was obtained in mrs broth supplemented with 0.5 % (w/v) caco3 at 6 h by gentle shaking. pcr walking using specific primers at the conserved region of class-ii bacteriocin resulted in 4 known genes of kld1, kld2, kl ...201627462508
antimicrobial activity of lactobacillus strains of chicken origin against bacterial pathogenss.this study was conducted to identify and evaluate the antimicrobial activity of some lactobacillus isolates of chicken origin. among 90 isolates 14 lactobacillus species were distinguished using maldi-tof mass spectrometry and 16s-ardra. the dominant species was l. salivarius (34.4%), followed by l. johnsonii (23.3%), l. crispatus (13.3%) and l. reuteri (11.1%). all lactobacilli were screened for antimicrobial activity against wild-type strains of salmonella enterica, escherichia coli, and clost ...201627762430
poultry blood from slaughterhouses: development of a biopreservation system to improve microbiological quality prior to transforming blood into by-products.the aim of this study was to investigate the use of indigenous lactic acid bacteria (lab) with specific additives as a biopreservation system (bs) for poultry blood during its storage in slaughterhouses. the bs consisted of two lab (enterococcus faecalis dspv 008sa or lactobacillus salivarius dspv 032sa) with 4 additives (lactose 2 g/l, yeast extract 0.4 g/l, ammonium citrate 0.4 g/l and nacl 1 g/l). after 24 h storage at 30ºc, lower counts of enterobacteria, coliforms, pseudomonas spp. and stap ...201627352105
in vitro inhibition of eimeria tenella sporozoite invasion into host cells by probiotics.the aim was to study the effects of probiotics isolated from the intestinal tract of livestock animals on eimeria tenella invasion into madin-darby bovine kidney (mdbk) cells in vitro. e. tenella sporozoites were purified and labeled with 5(6)-carboxyfluorescein diacetate n-succinimidyl ester before seeding on cell cultures, and invasion was evaluated by fluorescence microscopy. two protocols (a and b) were used. in protocol a, enterococcus faecium # 589 or lactobacillus salivarius subsp. saliva ...201627809987
using in vitro immunomodulatory properties of lactic acid bacteria for selection of probiotics against salmonella infection in broiler chicks.poultry is known to be a major reservoir of salmonella. the use of lactic acid bacteria has become one of successful strategies to control salmonella in poultry. the purpose of this study was to select lactic acid bacteria strains by their in vitro immunomodulatory properties for potential use as probiotics against salmonella infection in broiler chicks. among 101 isolated lactic acid bacteria strains, 13 strains effectively survived under acidic (ph 2.5) and bile salt (ranging from 0.1% to 1.0% ...201626799658
three novel candidate probiotic strains with prophylactic properties in a murine model of cow's milk allergy.food allergies can have significant effects on morbidity and on quality of life. therefore, the development of efficient approaches to reduce the risk of developing food allergies is of considerable interest. the aim of this study was to identify and select probiotic strains with preventive properties against allergies using a combination of in vitro and in vivo approaches. to that end, 31 strains of bifidobacteria and lactic acid bacteria were screened for their immunomodulatory properties in t ...201626729723
crystal structure of bile salt hydrolase from lactobacillus salivarius.bile salt hydrolase (bsh) is a gut-bacterial enzyme that negatively influences host fat digestion and energy harvesting. the bsh enzyme activity functions as a gateway reaction in the small intestine by the deconjugation of glycine-conjugated or taurine-conjugated bile acids. extensive gut-microbiota studies have suggested that bsh is a key mechanistic microbiome target for the development of novel non-antibiotic food additives to improve animal feed production and for the design of new measures ...201627139829
comparative study on the characteristics of weissella cibaria cmu and probiotic strains for oral care.probiotics have been demonstrated as a new paradigm to substitute antibiotic treatment for dental caries, gingivitis, and chronic periodontitis. the present work was conducted to compare the characteristics of oral care probiotics: weissella cibaria cmu (chonnam medical university) and four commercial probiotic strains. survival rates under poor oral conditions, acid production, hydrogen peroxide production, as well as inhibition of biofilm formation, coaggregation, antibacterial activity, and i ...201627999400
5-fluorouracil sensitivity varies among oral micro-organisms.5-fluorouracil (5-fu), a commonly used chemotherapeutic agent, often causes oral mucositis, an inflammation and ulceration of the oral mucosa. micro-organisms in the oral cavity are thought to play an important role in the aggravation and severity of mucositis, but the mechanisms behind this remain unclear. although 5-fu has been shown to elicit antibacterial effects at high concentrations (>100 µm), its antibacterial effect at physiologically relevant concentrations in the oral cavity is unknow ...201627296270
probiotics and refractory chronic spontaneous urticaria.background. in chronic spontaneous urticaria (csu) first-line therapy with an antihistamine-based regimen may not achieve satisfactory control in patients. thus, a continuing need exists for effective and safe treatments for refractory csu. aim. to evaluate the clinical efficacy and safety of an intake of a combination of 2 probiotics (lactobacillus salivarius ls01 and bifidobacterium breve br03) in patients with csu who remain symptomatic despite concomitant h1-antihistamine therapy. methods. t ...201627608474
development of putative probiotics as feed additives: validation in a porcine-specific gastrointestinal tract model.enforced restrictions on the use of antibiotics as growth promoters (agps) in animal production have prompted investigations into alternative feed additives in recent decades. probiotics are currently the main feed additive used in livestock. however, the selection of probiotic candidates relies on human-based methods and little is known about the verification criteria for host-specific selection. we investigated the probiotic potential of lactobacillus salivarius strains isolated from fed pig f ...201627633101
isolation and characterization of lactobacilli from human faeces and indigenous fermented foods for their potential application as probiotics.this study was conducted to select lactobacillus strains from various sources on the basis of their probiotic attributes, such as acid and bile tolerance, binding to intestinal cells, and antimicrobial activity. twelve isolates were obtained from human and food sources and were evaluated against standard probiotic lactobacillus rhamnosus gg (lgg). isolates were also studied for their antibiotic susceptibility. isolate lactobacillus fermentum gpi-6 showed the best survival profile at 0.3% and 1% ...201626928011
milk and blood biomarkers associated to the clinical efficacy of a probiotic for the treatment of infectious mastitis.previous studies have shown the efficacy of oral administration of selected lactobacilli strains to treat mastitis. the objective of this study was to find microbiological, biochemical and/or immunological biomarkers of the probiotic effect. women with (n=23) and without (n=8) symptoms of mastitis received three daily doses (10(9) cfu) of lactobacillus salivarius ps2 for 21 days. samples of milk, blood and urine were collected before and after the probiotic intervention, and screened for a wide ...201626925605
characterization of a new lactobacillus salivarius strain engineered to express ibv multi-epitope antigens by chromosomal integration.to obtain adhesive and safe lactic acid bacteria (lab) strains for expressing heterologous antigens, we screened lab inhabitants in intestine of tibetan chickens by analyzing their adhesion and safety properties and the selected lab was engineered to express heterologous antigen (utepi c-a) based on chromosomal integration strategy. we demonstrated that a new lactobacillu salivarius tcmm17 strain is strongly adhesive to chicken intestinal epithelial cells, contains no endogenous plasmids, is sus ...201626618736
prevention of infectious mastitis by oral administration of lactobacillus salivarius ps2 during late pregnancy.previous studies have shown that oral administration of lactobacilli can be an efficient approach to treat lactational infectious mastitis. in this trial, we have evaluated the potential of lactobacillus salivarius ps2 to prevent this condition when orally administered during late pregnancy to women who had experienced infectious mastitis after previous pregnancies.201626611780
detection of signature volatiles for cariogenic microorganisms.the development of a breath test by the identification of volatile organic compounds (vocs) emitted by cariogenic bacteria is a promising approach for caries risk assessment and early caries detection. the aim of the present study was to investigate the volatile profiles of three major cariogenic bacteria and to assess whether the obtained signatures were species-specific. therefore, the headspaces above cultures of streptococcus mutans, lactobacillus salivarius and propionibacterium acidifacien ...201626610336
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