Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
[effect of thermostabile exotoxin on the fertility of the fleas of the daughter generation]. 1977927383
[biological preparation, myasol, for the control of myiasis of sheep]. 1977929947
responsibility of 16s rna for the stimulation of polypeptide synthesis by spermidine. 1977326259
mutagenicity of bacillus thuringiensis exotoxin i. mammalian tests. 1977140159
a method for the isolation of asporogenic mutants of bacillus thuringiensis.asporogenic and oligosporogenic mutants of bacillus thuringiensis can be isolated by streaking the bacteria onto a solid medium, incubating at 42 degrees c, and picking small, raised areas of growth which appear on the streaks after 48 h.1978346187
inhibition of rna polymerase from bacillus thuringiensis and escherichia coli by beta-exotoxin.the characteristics of exotoxin inhibition of deoxyribonucleic acid (dna) dependent ribonucleic acid (rna) polymerase isolated from escherichia coli and bacillus thuringiensis were investigated. rna polymerase isolated from a variety of growth stages was partially purified and assayed using several different native and synthetic dna templates, and exotoxin inhibition patterns were recorded for each. although 8 to 20-h rna polymerase extracts of e. coli retained normal sensitivity to exotoxin (50 ...1978350363
determination of fatty acid compositions of bacillus cereus and related bacteria: a rapid gas chromatographic method using a glass capillary column.a rapid gas chromatographic method for the determination of fatty acid compositions of bacillus cereus and related bacteria is presented. by the use of a free fatty acid phase-coated glass capillary column, the complete separation of fatty acids, including the branched ones, was achieved. the method enables a more distinct differentiation of bacillus species than can be obtained with packed columns.1978416751
[radiosensitivity of the bacteriophages of aerobic sporulating microorganisms]. 1978417102
[a new variety of bacillus thuringinesis very toxic to mosquitoes: b. thuringiensis var. israelensis serotype 14].a new strain of b. thuringiensis is described, the flagllar antigen of which is a new one named h14. this strain produces unusual crystals, with all shapes and sizes, and shows a high toxicity for larvae of aedes aegypti.1978417869
toxicity of bacillus thuringiensis spores to the tobacco hornworm, manduca sexta.toxicity of bacillus thuringiensis spores to the tobacco hornworm, manduca sexta, is described. the numbers of larvae killed were in relation to spore dry weight. at a surface application of 6.8 ng/cm2, there was an 85 percent survival, but less than 50 percent survived at 68.2 ng/cm2. striking similarity of spores to parasporal crystals is revealed by slope of mortality curves, inhibition of stadial growth, and 50 percent lethal dose values based on protein content.1978623457
[comparative characteristics of spore-forming and asporogenic strains of bacillus thuringiensis].comparative characteristics of sporogenous and asporogenous bacillus thuringiensis strains is carried out. asporogenous strains are found to differ from wild type strains in a number of criteria, including colony morphology, character of growth on rich and poor media and uv-sensitivity. sporogenous strains form r colonies, they are more stable and more rare produce variants forming s colonies. s colonies are typical for asporogenous mutants, and under the cultivation in unfavourable conditions ( ...1978640382
degradation by eco r1 endonuclease of dna isolated from phages infecting bacillus licheniformis and bacillus thuringiensis.dna was isolated from lytic phages of two strains, bacillus licheniformis, a producer of bacitracin, and bacillus thuringiensis forming protein paracrystals with pronounced insecticidal effects. its sensitivity to eco r1 restriction endonuclease was determined. it was the aim of the work to find out whether these phages could serve as vectors in the transfer and possible amplification of genes of the two important industrial species of bacilli. approximate values of the molecular weight of dna o ...1978648976
the heat-stable exotoxin of bacillus thuringiensis. 1978648977
inactivation of bacillus thuringiensis spores by ultraviolet and visible light.the inactivation of bacillus thuringiensis spores and spores treated with two protectants, one proteinaceous and the other a commercial product, shade, at wavelengths of the near-ultraviolet and visible spectra and at 254 nm is described. determination of the inactivating wavelengths may be used to establish an efficient sunlight protective system for b. thuringiensis when used as a microbial insecticide.1978655707
[proteolytic enzymes bound to bac. thuringiensis crystals].it was demonstrated that crystals of entomopathogenic protein from bac. thuringiensis contain admixture of proteinase either adhered to their surface on inconponated into crystal lattice defects. a proteolytic action, particularly when enhanced by crystal dissolution, causes progressive degradation of crystal proteins with molecular weights of 140 000--129 000 down to the components with smaller molecular weights. this may, at least, partially account for the contradictions in the literature dat ...1978656507
bacillus thuringiensis delta-endotoxin: evidence that toxin acts at the surface of susceptible cells.enzymically activated delta-endotoxin of bacillus thuringiensis covalently bound to sephadex beads, has the same effect on insect cells in tissue culture as free toxin. the effect is prevented by antitoxin antibody and heat denaturation and is not due to a nonspecific protein effect, the beads, or toxin released from the beads. the toxin, therefore, probably acts at the cell surface.1978658294
studies on the mechanism of tetracycline resistance in mutants of bacillus thuringiensis. 1978667166
[hygienic standardization of dendrobacillin in the air of industrial enterprises and in the atmosphere]. 1978669319
[comparative phage sensitivity of r- and s-strains of bacillus thuringiensis]. 1978672690
transduction in bacillus thuringiensis.bacteriophage cp-51, originally reported as a generalized transducing phage for bacillus cereus and b. anthracis, has been shown to carry out generalized transduction in several strains of b. thuringiensis. a newly isolated phage, cp-54, which has a broader host range than cp-51, also mediates generalized transduction in b. thuringiensis. cp-51 and cp-54 are similar in size and morphology and are related serologically, but they are not identical. cp-54 is more cold labile than cp-51, and, as wit ...1978677877
occurrence of two serologically distinct groups within bacillus thuringiensis serotype 3 ab var. kurstaki. 1978681772
an image reversal process for electron microscope film.an improved technique has been developed to produce a positive image directly on some standard electron microscope films (kodak 4489, so 163, and ilford electron microscope film). this technique has been useful for processing micrographs of freeze-etched and shadowed specimens, and eliminates the need for producing positive image transparencies before printing.1978682183
partial characterization of a cubic bacillus phage.partial characterization of a cubic bacillus phage. can. j. microbiol. 24: 986--993. phage bam35 is an icosahedron of about 63 nm in diameter. it has a double capsid with spikes at the vertices, and a tail which seems to appear upon nucleic acid ejection. the phage contains dna and, probably, lipids which seem to be located in the inner coat. the phage is bacillus-specific, uv- and lipase-resistant, and sensitive to heat, chloroform, and ether. the latent period is 50 min and the burst size is 3 ...1978688106
isolation of bacillus thuringiensis for microbiological control of insects.eleven of 180 local isolates, belonging to bacillus thuringiensis, were tested for their pathogenicity to pinkbollworm larvae (pectinophora gossypiella). three strains, namely rs-25, rs-35, and rs-45, showed a positive insecticidal activity. their activities based on the crystalline inclusion bodies. the ld50 (hours) for larvae was 8, 12, and 6 hours, respectively.1978696044
purification of the protein crystal from bacillus thuringiensis by zonal gradient centrifugation.a method is described for the large-scale purification of the bacillus thuringiensis protein crystal by zonal gradient centrifugation. nabr gradients are employed in a beckman j21-b centrifuge equipped with a jcf-z rotor.1978708033
[isolation and identification of bacillus thuringiensis from environmental objects]. 1978710921
possible origin and function of the parasporal crystal in bacillus thuringiensis. 1978718701
metaphase arrest in the bone marrow cells of rattus norvegicus by the beta-exotoxin of bacillus thuringiensis.the beta-exotoxin, thuringiensin a, from bacillus thuringiensis var. thuringiensis has been found to be c-mitotic in the bone marrow cells of rattus norvegicus. this exotoxin therefore appears to be a potential tool for metaphase arrest in karyological studies. the delta-endotoxin protein has no similar activity.1978754420
[toxicity of bacillus thuringienses var. israelensis for larvae of aedes aegypti and anopheles stephensi].the comparative study of the larvicidal action of b. thuringiensis var. israelensis on a. aegypti and a. stephensi shows the greater sensitivity of a. aegypti, with 100% of mortality in 30 to 40 min. at high doses. but, for both species of mosquito, the toxicity of these bacteria is very high, as shown by the mortality regression curves and by the lc50. this toxicity is associated with the crystals and can be extracted from them by dilute alkali solution, like the general case of the other serot ...197896979
[cytological study of the action of bacillus thuringiensis var. israelensis on mosquito larvae].a comparison is made between the cytopathological effects of b. thuringiensis var. israelensis on aedes aegypti larvae and the cytopathological effects of the other varieties of b. thuringiensis on lepidoptera larvae. the same primary action is observed, with the loss of integrity of the gut epithelium, the cells of which appear swollen, distorted and finally burst.197897020
[study of lysogeny in bacillus thuringiensis and b. cereus].forty-eight strains of bacillus thuringiensis and 12 strains of b. cereus were treated with ultraviolet light and mitomycin c. the former agent was the more effective inducer. bacillus thuringiensis produces at least seven different phage particles with long, non-contractile tails. the frequencies of lysogeny and polylysogeny are 83 and 25% respectively. morphologically defective phages occur in 25% of strains, whereas five of them produce low molecular-weight bacteriocins. one strain of b. cere ...197898223
[modes of tetracycline resistance in bacillus thuringiensis].the sensitive strain and tetracycline-resistant mutants exhibit two types of growth curves after addition of tetracycline. the level of resistance of these strains is enhanced by a short period of preincubation with a non-inhibitive concentration of tetracycline.197899258
[relative sensitivity of various cell cultures to the thermostable exotoxin of b. thuringiensis].the "thermostable" b. thuringiensis exotoxin is active on cell cultures of mammals "in vitro", except on the kb strain from a human tumor. the primary cultures are the most sensitive: first, with monkey kidney cells, the growth is inhibited by 0.1 mg of toxin per ml; next, the young rabbit kidney cells react to 0.25 mg of toxin per ml. the established lines of cells come last: human diploid cells (lyon 4) and heteroploid cells (bhk21c13), with the same active dose of 1 mg of toxin per ml. no pro ...1978100834
deoxyribonucleic acid relatedness between bacillus anthracis, bacillus cereus and bacillus thuringiensis. 1978105229
radiosensitivity of bacteriophages of aerobic spore-forming microorganisms.under conditions of the direct action of radiation on seven original phages of aerobic spore-forming bacteria and four standard phages of e. coli, it was established that they possessed different sensitivity to gamma radiation and were distributed into four groups. the phages of e. coli of the t series, especially t2 phage, proved the most sensitive. the phages of aerobic spore-forming bacilli were characterized by greater resistance to the action of gamma radiation, which is evidently associate ...1978105763
[toxins and enzymes of several species of bacillus, especially of the b. cereus-thuringiensis group (author's transl)].in the examined strains the production of following toxins or enzymes was determined by bioassay or by semiquantitative and routine diagnostical tests: delta-endotoxin, alpha-exotoxin, beta-exotoxin hemolysin, phospholipase c, proteinase. the production of delta-endotoxin (= a parasporal crystal toxic for several insects) is the only character in that b. thuringiensis differs from b. cereus. other biochemical features as production of so-called alpha-exotoxin (= soluble toxic protein), hemolysin ...1979112804
virulence factors in bacillus thuringiensis: purification and properties of a protein inhibitor of immunity in insects.we have previously shown that bacillus thuringiensis subsp. alesti, serotype 3, produces two extracellular inhibitors of the immune system of saturniid pupae (designated inhibitors a and b; edlund et al., 1976). starting from the culture supernatant of a new mutant of b. thuringiensis with a decreased extracellular proteolytic activity, we have now purified immune inhibitor a(ina). the procedure described consists of three steps: ultrafiltration, precipitation with ammonium sulphate and chromato ...1979118233
[toxicity of bacillus thuringiensis var. israelensis for simulium larvae, vectors of onchocerciasis].on field trials in infested streams, the primary powder r 153-78 made of b. thuringiensis var. israelensis with a potency of 3,000 i.t.u. aedes aegypti/milligramme is very toxic for blackfly larvae, especially for simulium damnosum s.l. larvae. the lethal concentration 100, in 24 h. is 0.2 x 10(-6) for a 10 min. treatment. this high toxicity, specific for diptera larvae such as mosquitoes and blackflies, is related to the special characteristic of the bacterial crystal protein which has a clear ...1979118818
[determination of the antibacterial activity of various mycotoxins using bacillus thuringiensis (berliner)].antibacterial activity of pure preparations of mycotoxins, was studied against strains of bacillus thuringiensis (berliner). most of fungal metabolites used provokes: 1) growth bacterial inhibition similar to lethal effect of antibiotics 2) enlargment of cell volume; this cell abnormality induction resembles those obtained with mitomycin c. the bioassay of fungal toxins with b. thuringiensis can be recommended as a sensitive technique to supplement chemical characterization. by using specific ce ...197936567
mechanism of inhibition of active potassium transport in isolated midgut of manduca sexta by bacillus thuringiensis endotoxin.after incubation at ph 10 or higher, bacillus thuringiensis spores and endotoxin, at concentrations above 0.1 iu/ml, affected transport parameters in the isolated midgut of manduca sexta larvae. (toxic activity was lost during roughly 1 week at ph 11.) about 60% of the short-circuit current was inhibited, and the remainder was reversibly inhibited by anoxia. electrical resistance was reduced by about 55% and oxygen uptake stimulated by about 30%. influx of potassium from blood-side to lumen-side ...197943881
toxicity of parasporal crystals of bacillus thuringiensis subsp. israelensis to mosquitoes.toxicity of bacillus thuringiensis subsp. israelensis (onr-60a/who 1897) parasporal crystals to three medically important mosquito larvae is described. the numbers of larvae killed are in relation to crystal dry weight. the crystals are lethally toxic to aedes aegypti linnaeus (mean 50% lethal concentration [lc50] = 1.9 x 10(-4) micrograms/ml), culex pipiens var. quinquefasciatus say (lc50 = 3.7 x 10(-4) micrograms/ml), and anopheles albimanus wiedemann (lc50 = 8.0 x 10(-3) micrograms/ml). purfi ...197944177
[effect of ph on the growth of bacillus thuringiensis].the effect of ph on the growth phase parameters was studied with bacillus thuringiensis. the experimental data obtained were used to draw the regression equation describing the spore titre as a function of ph. as a result of analysing the equation coefficients and the effect of ph on the growth phase parameters, a ph profile has been proposed which favours an increase in the titre of spores. the profile decreases the rate of oxygen uptake at the exponential growth phase and increases the rate of ...197934081
[comparative sensitivities of six different species of mosquitoes to bacillus thuringiensis var. israelensis]. 197934488
on the formation of crystal proteins during sporulation in bacillus thuringiensis var. thuringiensis.the processes of sporulation and crystal formation in bacillus thuringiensis could be desynchronized, using short term temperature shift-up conditions or addition of chloramphenicol at the beginning of sporulation. isolation of a sp-, cr+ mutant, blocked at early stage of sporulation further confirmed that crystal formation can occur in the absence of sporulation. the presence of one of the two antigenic subunits of the crystal protein in chloramphenicol treated cultures and both of them in cult ...1979426602
[protein composition of crystals (delta-endotoxin) of different serotypes of bac. thuringiensis].the crystals of entomopathogenic protein from bac. thuringiensis contain admixtures of proteinases adhering to their surfaces. a newly developed technique of protease inactivation allowed to estimate the true protein composition of the crystals of various strains of bac. thuringiensis. it was shown that the crystals of all strains (with the exception of v and viii) are composed of only one protein with molecular weights of 145,000, 135,000 and 130,000, depending on the strain. the crystals of se ...1979435578
[bacillus thuringiensis plasmid dna].plasmid dnas were isolated from bacillus thuringiensis and their physico-chemical properties were studied. the dnas were purified using ultracentrifugation in a cscl2 density gradient. the molecular weight of these extrachromosomal dnas determined by electrophoresis in 0.6% agarose gel and by electron microscopy was 6 . 10(6)--10 . 10(6) daltons; a dna with a molecular weight of 90 . 10(6) daltons was also found. digestion with restriction endonuclease ecori yielded fragments of the plasmid dnas ...1979440159
[proteinases in growth and spore formation of bacillus thuringiensis].some serine proteases and leucine aminopeptidases were detected inside and outside the cells during the analysis of three crystalline and two acrystalline strains of bac. thuringiensis var. galleriae. the data obtained on the protease formation during growth and sporulation and the level of their activity are indicative of intracellular proteases involvement in spore- and crystal formation. the enzymes isolated from the culture medium do not probably take part in these processes. the intracellul ...1979454710
[antibacterial activity of zearalenone].zearalenone, a mycotoxin secreted by fusarium sp. and gibberella zeae, shows a narrow range of antibacterial activity limited to some gram-positive aerobic spore-forming bacteria. in bacillus thuringiensis (berliner), a highly sensitive species, this activity is characterized by a decrease of cellular division and induction of atypical cells. these effects resemble those obtained with two other mycotoxins which possess a lactone structure: aflatoxin b1 and patulin.1979455155
[genetic and radiobiologic characteristics of bacillus thuringiensis tg13 phage]. 1979456889
[fibrinolytic activity of several variants of bacillus thuringiensis]. 1979461377
[amination and biosynthesis of glutamate by r- and s-forms of bacillus thuringiensis].these studies were designed to determine the biochemical nature of the bacillus thuringiensis growth being dependent on glutamate during cultivation in a minimal medium. this is possible to be due to the absence of enzymes which catalyze glutamic acid synthesis by direct amination of alpha-ketoglutaric acid, glutamate dehydrogenase and glutamate synthase, and a decrease in the activity of the enzyme catalyzing amination of pyruvic acid, alanine dehydrogenase. it has been shown that the lack of g ...1979470635
autoradiographic study of the localization and evolution of growth zones in bacterial colonies.incorporation of [3h]leucine in the bacteria of 18 to 48 h-old colonies of pseudomonas aeruginosa, pseudomonas putida, bacillus thuringiensis, staphylococcus aureus and escherichia coli enabled the localization of bacterial multiplication sites by means of autoradiography of sagittal sections. in colonies where fast diameter expansion occurred, all the bacteria from the peripheral corona contributed to peripheral growth; in colonies where the expansion was slower, the growth rate of the bacteria ...1979479831
[bacteriocin-like factor in bacillus thuringiensis].the paper describes bacillus thuringiensis var. galleriae strains possessing a bacteriocin-like factor (factor k) which inhibits the cultures of a number of serotypes of bac. thuringiensis. the k+ cultures exhibit no antibacterial effect on other gram-positive and gram-negative microorganisms. the maximal level of factor k is found by the 8--9th hour of cultivation. no correlation has been established between the strucutred elements in the preparations of k+ cultures and the antibacterial activi ...1979481284
toxicity of parasporal crystals of bacillus thuringiensis to the indian meal moth, plodia interpunctella.toxicity of bacillus thuringiensis parasporal crystals to the indian meal moth, plodia interpunctella, is described. the numbers of insects killed were in relation to crystal dry weight. mortality was determined by comparing adult emergence in diets treated with crystals to emergence in untreated diets. there was only a 30% survival at an application of 0.414 microgram/cm2, and the mean 50% lethal concentration value was found to be 0.299 microgram/cm2. the use of emergence data has provided a r ...1979485134
[hygienic basis for regulating the use of bacterial insecticides in agriculture and the permissible residues of their producer, bac. thuringiensis, in plant products]. 1979499875
mutagenicity of bacillus thuringiensis exotoxin iii. sister chromatid exchange in rats in vivo. 1979500386
converting bacteriophage for sporulation and crystal formation in bacillus thuringiensis.bacteriophage tp-13, a converting phage for sporulation and crystal formation in bacillus thuringiensis, was isolated from soil. the phage converted anoligosporogenic (sporulation frequency, 10(-8), acrystalliferous mutant to spore positive, crystal positive at a high frequency. each plaque formed by tp-13 in a lawn of sensitive cells contained spores and crystals. these spores were heat stable, and each one was capable of producing a plaque from which tp-13 could be reisolated. conversion of ce ...1979500567
toxicity of bacillus thuringiensis toward aedes aegypti larvae. 1979501129
[electron microscopic study of the interaction between phages and bacillus thuringiensis cells].the interaction of phages belonging to different morphological groups with the cells of bacillus thuringiensis var. galleriae r and s variants was studied. no adsorption of phages tg11 and tg18 on the cells of r variant was found upon infection in a liquid medium. what is characteristic of phage tg11 is that it is predominantly adsorbed at the poles of s variant cells. phage tg18 particles are uniformly distributed along the perimeter of s variant cells. phage tg13 is adsorbed on the both varian ...1979502910
who looks at bacteria for vector control. 1979503213
study on the possibility of adopting a complex method in blattella germanica (l.) control on board ships.campaings to control the german cockroach, blattela germanica, were conducted at 14-day intervals over a period of 5.5 months on two merchant vessels of similar size and type. on one ship only the chemical "gertoks", carbamate insecticide containing 1% propoxur was applied, and on the second ship two preparations simultaneously, a chemical and a biological--"biotrol 25 w" containing bacillus thuringiensis. - in order to assess the success of the control, the extent of infestation in the accomoda ...1979506564
[partial purification and properties of proteases of bitoxibacillin].the paper describes properties of proteases a and b isolated from the biological insecticide bitoxibacillin by sulphate precipitation and sephadex g-75 gel filtration. proteases a and b of bitoxibacillin belong to the neutral bacterial proteases. ph optimum was found to be 6.0 and 7.5 for detection of proteolytic activity of protease a and protease b, respectively. thermal stability of proteases a and b was similar and increased by 25% upon addition of cacl2. both proteases were inhibited with e ...1979514993
interchain crosslinks in the entomocidal bacillus thuringiensis protein crystal. 1979520581
purification of the insecticidal toxin from the parasporal crystal of bacillus thuringiensis subsp. kurstaki. 1979526269
[effect of certain organophosphorus insecticides on spores of the bacillus thuringiensis group of bacteria]. 1979530120
phagocytosis-stimulating mediators in insects.the ability of insect blood cells to ingest all kinds of synthetic particles and also a wide range of microorganisms in a very short time after injection has up to now been regarded as a phagocytic function without any humoral mediators. in a phagocytosis model with latex beads and nonhagocytosable cells of bacillus thuringiensis subtoxicus, we are able to demonstrate the existence of lymphokine-like factors, which intervene in cellular defence reactions of insect. the following results were obt ...1979543373
[effect of high doses of the toxins from bacillus thuringiensis strains on mice and sheep].the investigation on bac. thuringiensis toxines applied orally and subcutaneously on experimental white mice and on sheep proved that high doses of the dipel endotoxin are most toxic for mice of all strains (no. 862, 2e, 171) and spores and crystals of strain nd-1 used in dipel injection. the endotoxin of strain 862 applied orally to sheep at a dose of 10 cm3/kg body weight caused slight changes only to some paraclinical indices, namely: total protein, carotene, etc. exotoxin had none or only sl ...1979545851
[using a combined transcription-translation system for determining the role of cryptic plasmids of bacillus thuringiensis].the possibility of entomocyde crystal protein synthesis was studied using a heterological cell-free system with bacillus thuringiensis plasmid dna as template. the high level of template activity is usual for bac. thuringiensis plasmid dna. immunochemical studies of the in vitro synthesized polypeptides showed that bac. thuringiensis plasmid dna does not direct crystal protein synthesis.1979547175
[pigment-forming mutants of bacillus thuringiensis]. 1979547696
[plasmids and crystal formation in bacillus thuringiensis]. 1979547698
biological and immunological properties of crystalline proteins from b. thuringiensis var. galleriae and var. insectus.the antigenic and toxic properties of the crystalline proteins from two varieties of b. thuringiensis (var. galleriae and var. insectus) were studied. methods of double diffusion in agar and two-dimensional disc immunoelectrophoresis revealed four antigens in the crystalline protein from var. galleriae and three in that from var. insectus. a definite correlation was found between the toxicity of the crystalline protein and its capacity to interact with antiserum in a precipitation reaction in ag ...1979549674
cytotoxic activity of the delta-endotoxin of bacillus thuringiensis var thuringiensis (berliner) on fibrosarcoma in swiss mice. 1979549845
[effect of bacterial insecticides on soil self-purification processes and on the survival in the soil of pathogenic enterobacteria with the example of salmonella typhimurium]. 1979387535
structure of cloned ribosomal dna cistrons from bacillus thuringiensis.a library of b. thuringiensis dna has been prepared by using the plasmid pbr322 as a cloning vehicle and e. coli as a host cell. by screening this collection with specific probes, 17 clones were identified whose hybrid plasmids contain rrna genes of b. thuringiensis. several of these plasmids have been mapped with restriction endonucleases and by dna-rna hybridization. by using maps of overlapping fragments, we have been able to establish an overall map of the ribosomal gene cluster.1979388353
contamination of the deep tissues of carcasses by bacteria present on the slaughter instruments or in the gut. 1979222722
[hygienic basis of processes utilizing plant products grown using bac. thuringiensis-based bacterial insecticides]. 19807203061
crystal-forming proteins of bacillus thuringiensis. the limited hydrolysis by endogeneous proteinases as a cause of their apparent multiplicity.the crystals of the entomocidal protein of bacillus thuringiensis are admixed with proteinases that in the course of their dissolution cause gradual degradation of the "genuine" crystal-forming protein components (i.e. the primary biosynthetic products) to products of lower molecular weight. this phenomenon might explain at least partially the contradictory data on the molecular parameters of the crystal-forming proteins. preliminary inactivation of the proteinases adsorbed on the crystals allow ...19806994717
ultrastructure, physiology, and biochemistry of bacillus thuringiensis. 19807000441
a. m. heimpel (1923-1979): an appreciation. 19807003024
deposit assessment of bacillus thuringiensis formulations applied from an aircraft.deposit assessment after aerial spraying with bacillus thuringiensis (b.t.) formulations consists of the analysis of the splash of droplets on kromekote papers and determining the number of bacterial colonies growing on nutrient agar medium petri dishes. however, neither of these two methods provides a number of spores deposited although this figure is essential to determine treatment efficiency. both methods are evaluated with regard to this need and a new approach is suggested.19807011511
the crystalline layers in spores of bacillus cereus and bacillus thuringiensis studied by freeze-etching and high resolution electron microscopy.the comparative morphology of spores from bacillus cereus, b. thuringiensis s-9 (wildtype), b. thuringiensis hb 9-1 (acrystaliferous mutant) and b. finitimus, was studied by the freeze-etching technique. particular attention was given to the three crystalline layers found in these spores, the pitted layer, the parasporal layer, and the basal layer of the exosporium with lattice constants of 9.2 nm, 5.8 nm and 8.0 nm respectively. the parasporal layers, corresponding to the fraction of fl of sche ...19806766865
[relationship between spore formation and exoproteinase synthesis in bacillus thuringiensis].the localization of exoproteinase in the conventionally "periplasmic" space of bacillus thuringiensis was established. the enzyme located at the outer surface of the membrane was found, in the course of sporogenesis, within the spore where it could fulfill various functions. the role of glutamine synthetase was determined in the regulation of exoproteinase synthesis and sporogenesis. the both processes were inhibited in the conditions suppressing the activity of glutamine synthetase.19806106151
the isolates of bacillus thuringiensis serotype 10 with a highly preferential toxicity to mosquito larvae. 19806108343
[evaluation of the larvicidal activity of bacillus thuringiensis serotype h14 on mosquitoes in natural breeding places].the experiments conducted with bacillus thuringiensis var. israelensis, serotype h14, in nature, show that this bacillus has a specific and extremely high larvicidal power against the larval population of culicidae. it operates very quickly but its action seems relatively short-time lasting. considering the increasing resistance of larvae to organophosphorus compounds, this new serotype presents a particular interest for the control of mosquitoes.19806112072
[laboratory and field evaluation of the mosquito larvicidal effect of bacillus thuringiensis var. israelensis (author's transl)]. 19806113102
[dynamics of nucleic acid and protein content in bacillus thuringiensis cells].the content of dna, rna and protein was assayed in bacillus thuringiensis var. insectus guk at different growth stages and under different cultivation conditions. the content of rna and protein was found to be maximal in the cells at the end of the lag phase and at the beginning of the exponential phase; then, this content decreased with aging of the culture. the content of dna in the strain depended on the conditions of cultivation, and reached amounts corresponding to 10-13 chromosomes when th ...19806156386
parasporal crystals produced by oligosporogenous mutans of bacillus thuringiensis (spo-cr+).six oligosporogenic (spo-) mutant strains of bacillus thuringiensis were selected from survivors of treatment with n-methyl-n'-nitro-n-nitrosoguanidine. each strain was blocked at or before stage ii of spore development, but all produced typical bipyramidal-shaped crystalline inclusion bodies. toxicity of the paraporad typical bipyramidal-shaped crystalline inclusion bodies. toxicity of the parasporal endotoxin isolated from the mutant strains was assayed by an in vitro technique using cultured ...19806247047
[properties of e. coli, salm. typhimurium and cl. perfringens strains isolated from soil containing bacterial insecticides based on bac. thuringiensis]. 19806247250
[plasmids of bacillus thuringiensis var. galleriae strain 612].we studied bacillus thuringiensis var galleriae, strain 612 plasmids. b. thuringiensis cells contain double-stranded plasmid dna molecules (ranging of about 12% from total dna content) with buoyant density 1.59 g/cm3. plasmid dna content was constant during the exponential and stationary phases of bacterial growth. the plasmid fractions consist of dna molecules with molecular weights of 5.9 x 10(6), 10.0 x 10(6), and 110.9 x 10(6) daltons (pvd1, pvd2 pvd3, respectively). endonuclease ecori cuts ...19806252447
[lysogeny in bacillus thuringiensis].different strains of several bacillus thuringiensis serotypes were analyzed for lysogeny. all of the studied cultures released phages. phages with an isometric capsid and a noncontractible tail were detected in preparations of the majority of the cultures. the growth of a group of related phages was limited upon lysogenization of the galleriae serotype with tm2 phage isolated from a culture of the morrisoni serotype. the physico chemical parameters of the phages were determined.19807207263
[growth characteristics of polylysogenic culture of bacillus thuringiensis subspecies galleriae in a chemostat]. 19807207265
generalized transduction in bacillus thuringiensis var. berliner 1715 using bacteriophage cp-54ber.a phage isolated from lysates of phage cp-54 grown on bacillus cereus 569 and selected on the basis of its ability to infect bacillus thuringiensis var. berliner 1715 (serotype i) was designated cp-54ber. phages cp-54ber and cp-54 were similar in size, morphology, cryosensitivity and stabilization by dimethyl sulphoxide. they showed significant differences with regard to inactivation by specific antiserum, adsorption to the berliner strains and host range. phage cp-54ber was able to mediate gene ...19807252480
[safety of the use of the serotype h-14 of bacillus thuringiensis for the non-target fauna of the mosquito breeding places of the french mediterranean coast]. 19807312401
[immediate larvicidal activity and residual action of the endotoxin of the serotype h-14 of bacillus thuringiensis in 2 mosquito biotopes on the french mediterranean coast].the primary powder r.153.78 of the serotype h.14 of b. thuringiensis has been evaluated in small field plots against larvae of ae. detritus and cx. pipiens.. the minimal concentration of the primary powder inducing a complete mortality was 0.1 mg/1 against ae. detritus and 0,4 mg/1 against cx. pipiens, corresponding at dosages of respectively about 310-420 and 1240-1680 international toxic units per litre. the residual larvicide effectiveness was close to nil in spite of the chemical stability o ...19807312402
[activity of the serotype h-14 of bacillus thuringiensis against the principal species of anthropophilic mosquitoes of the french mediterranean coast]. 19807312403
patterns of plasmid dna in crystalliferous and acrystalliferous strains of bacillus thuringiensis. 19807335824
[sanitary microbiological standards for bac. thuringiensis, a producer of bacterial insecticides, in plant products].survival of bac. thuringiensis, producers of bacterial insecticides in plant products, was studied in the process of culinary and technological treatment (thermal treatment, preservation, etc.), depending on the extent of dissemination of plant raw material, type of the plant product and pattern of culinary treatment. a study was also made of the viability of the above species in finished products and dishes exposed to varying storage temperatures. according to the data obtained, the use of plan ...19807405137
sporeless mutants of bacillus thuringiensis. iii. the process of crystal formation.in order to investigate the formation of the parasporal crystal of b. thuringiensis with special reference to the spore, sequential ultrastructural analysis of sporulation was performed using a sporeless mutant strain (sp-) as well as its parent wild strain (sp+). from the logarithmic growth to the end of forespore formation, the same sequential process of sporulation proceeded in both strains and a forespore with double membranes appeared. thereafter, subsequent sporulation in the sp- strain wa ...19807414596
bacillus thuringiensis parasporal crystal toxin: dissociation into toxic low molecular weight peptides. 19807417316
purification and properties of phosphatidylinositol-specific phospholipase c of bacillus thuringiensis.a phosphatidylinositol-specific phospholipase c was purified from the culture broth of bacillus thuringiensis to a homogeneous state as indicated by polyacrylamide gel electrophoresis. specific activity of purified enzyme was 312 units/mg, and the recovery of the enzyme activity was 27.2%. the purified enzyme (molecular weight: 23 000 +/- 1000) was maximally active at ph 7.5 and not influenced by edta. the enzyme specifically hydrolyzed phosphatidylinositol, but did not act on phosphatidylcholin ...19807417468
Displaying items 301 - 400 of 7316