Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| specificity of utilization of human salivary proteins for growth by oral streptococci. | 1979 | 287558 | |
| factors influencing the deposition and growth of oral organisms on hard surfaces. | 1979 | 287687 | |
| translocation of the pre-synaptic complex formed upon dna uptake by streptococcus sanguis and its inhibition by ethidium bromide. | donor dna in its initially bound, single-stranded form exists in a chromosomally-unassociated complex where it is resistant to exogenous dnase i but sensitive to micrococcal nuclease. most of the complexes are readily recuperable from the supernatant of recipients converted into spheroplasts. subsequent to formation of this superficially located complex, donor dna progressively associates with the recipient chromosome into which it is eventually integrated. treatment of recipients with ethidium ... | 1979 | 287850 |
| fragility of salivary aggregates of streptoccus mutans. | 1979 | 291486 | |
| experiments with pulp capping. | 1979 | 291861 | |
| structure of extracellular water-soluble polysaccharides synthesized from sucrose by oral strains of streptococcus mutans, streptococcus salivarius, streptococcus sanguis and actinomyces viscosus. | 1979 | 292363 | |
| comparative study of two variants of the mouth streptococcus sanguis with different colonial morphologies and abilities to adhere. | 1979 | 295202 | |
| bacterial endocarditis: a clinical report. | 1979 | 286207 | |
| release of lipoteichoic acid from streptococcus sanguis: stimulation of release during penicillin treatment. | the spontaneous and the penicillin-stimulated release of water-soluble, glycerol-labeled polymers was compared in streptococcus sanguis. in contrast to the spontaneous release occurring in exponentially growing or stationary-phase bacteria, penicillin-treated cells released the bulk of these polymers, and they were not replenished by synthesis during antibiotic treatment. furthermore, a major portion of the extracellular polymers was characterized as acylated lipoteichoic acid. | 1979 | 438118 |
| genetic transformation in streptococcus sanguis. spontaneous and induced competence of selected strains. | nine strains of streptococcus sanguis were examined for competence in genetic transformation with streptomycin resistance (str-r) as marker. eight strains belonged to serogroup h and one to the newly-described serogroup w. seven of the strains, one of which was the reference strain nctc 7868 (strain challis), were competent with str-r dna from strain challis. strains nctc 9124 (strain wicky) and 480 were incompetent. the efficiency of transformation was examined in four different media. use of t ... | 1979 | 375670 |
| [persistency and resistance of streptococci isolated from periodontal pockets]. | intradermal injection of cell walls or cell wall constituents (peptidoglycane) of streptococcus sanguis ii in experimental animals caused a similarly severe inflammatory reaction as with streptococcus a. the three "viridans" species of streptococci proved to be resistant to complement (active serum) as well as to lysozyme and were superior to streptococcus a in their capacity for resistance to another type of muralytic enzyme isolated from streptomyces albus. the new acylureido penicillins (mezl ... | 1979 | 374053 |
| genetic transformation in streptococcus sanguis. distribution of competence and competence factors in a collection of strains. | forty-one strains of streptococcus sanguis (37 of serogroup h and four of the newly-described serogroup w) were examined semiquantitatively for genetic transformation with streptomycin as marker. the material comprised eight reference laboratory strains and 33 recent isolates. eighteen strains (16 of serogroup h and two of w) showed spontaneous competence in genetic transformation (without added competence factor, i.e. culture filtrate.). individual culture filtrates from 19 spontaneously compet ... | 1979 | 375671 |
| aggregation and adherence of streptococcus sanguis: role of human salivary immunoglobulin a. | fourteen freshly isolated strains of streptococcus sanguis were obtained from the dental plaque of five healthy adults. whole saliva was collected concomitant with the plaque isolates from the five subjects, and a second whole saliva sample was collected 10 weeks later. all possible combinations of the first five saliva samples, the second five saliva samples, and 14 strains of bacteria were tested for aggregation. of the 140 combinations examined, 108 of 140 (77%) of the strains aggregated with ... | 1979 | 528050 |
| influence of preformed antibody on experimental streptococcus sanguis endocarditis. | the influence of preformed, anti-whole organism antibody on the development of streptococcus sanguis endocarditis was examined in both in vivo and in vitro systems. antibody prevented, rather than potentiated, endocarditis in rabbits. the infectious dose in 30 control animals was 10(6.5) +/- 0.33 (mean +/- standard deviation); this increased to 10(7.71 +/- 0.05 in 36 immunized animals (p less than 0.01). no differences in bacterial clearance mechanisms were apparent between groups. antibody also ... | 1979 | 500187 |
| biosynthesis of glycosylated glycerolphosphate polymers in streptococcus sanguis. | two types of glycosylated glycerolphosphates were synthesized when a particulate enzyme prepared from streptococcus sanguis was incubated with [3h]-phosphatidylglycerol and uridine diphosphate-[14c]glucose in the presence of mgcl2. the first type was extractable with saline and contained no fatty acid. the second type was pellet bound and could be extracted with 0.1% sodium dodecyl sulfate. both types of polymers were purified and partially characterized. the first type of polymer was fractionat ... | 1979 | 500562 |
| antimicrobial susceptibilities of species of viridans streptococci. | 1979 | 501146 | |
| effect of age on the membrane lipid composition of streptococcus sanguis. | 1979 | 508749 | |
| serologic studies of streptococcus intermedius, streptococcus constellatus, and streptococcus morbillorum by crossed immunoelectrophoresis. | a reference antigen-antibody system for streptococcus intermedius, streptococcus constellatus, and streptococcus morbillorum was established with crossed immunoelectrophoresis. a comparison of s. intermedius, s. constellatus, and s. morbillorum with crossed immunoelectrophoresis and crossed immunoelectrophoresis with intermediate gel indicated that s. intermedius and s. constellatus are closely related antigenically with as many as six common cytoplasmic antigens. s. morbillorum was antigenicall ... | 1979 | 453098 |
| lymphoproliferative responses induced by streptococcal antigens in recurrent aphthous stomatitis and behcet's syndrome. | 1979 | 455802 | |
| linkage analyses of extracellular glucans from streptococcus sanguis and streptococcus mitior. | similar alpha-(1-->6) linkage-rich, soluble, extracellular glucans have been isolated from six strains of two genetically distinct groups of streptococcus sanguis and three strains of streptococcus mitior. | 1979 | 457265 |
| streptococcal infection of endocardial and other intravascular vegetations in rabbits: natural history and effect of dexamethasone. | experiments were designed to study the natural history of infection in different parts of the vascular system. sterile vegetations were produced in rabbits by placing catheters in the inferior vena cava, tricuspid or aortic valves, and thoracic or abdominal aorta and then were infected by the intravenous inoculation of streptococcus sanguis. at 1 day after bacterial challenge, all vegs were infected, mean bacterial densities being highest in the vegs of the aortic and tricuspid valves. by 14 day ... | 1979 | 457283 |
| specificity of coaggregation reactions between human oral streptococci and strains of actinomyces viscosus or actinomyces naeslundii. | coaggregation reactions between actinomycete and streptococcal cells occurred frequently when human strains of actinomyces viscosus or a. naeslundii were mixed with human isolates of streptococcus sanguis or s. mitis, but were infrequent with other oral actinomycetes and streptococci. two groups of actinomycetes and four groups of streptococci were defined by the patterns of their coaggregation reactions and by the ability of beta-linked galactosides (i.e., lactose) to reverse these reactions. c ... | 1979 | 468376 |
| molecular characterization of unique deletion mutants of the streptococcal plasmid, pam beta 1. | 1980 | 6100905 | |
| electron microscopic visualization of extracellular polysaccharides on the cell wall of some streptococci. | the thickness of the acid polysaccharide capsule varied remarkably among the studied streptococci. in some strains, projections of the capsule and sloughing of the polysaccharide were observed in the medium. | 1980 | 6153663 |
| deoxyribonucleases of streptococcus sanguis. | three dnases, designated 1, 2 and 3 have been isolated from lysates of old (incompetent in genetic transformation) and logarithmic phase (competent) streptococcus sanguis strain challis cells. these dnases have different electrophoretic mobilities and immunological properties. influence of some activators and inhibitors on their activity was determined. molecular weights of 3 dnases are in the range of 52,000--57,000. all these enzymes are endonucleases which degrade native dna; thermally denatu ... | 1980 | 6155051 |
| lethal effect of a heterologous murein hydrolase on penicillin-treated streptococcus sanguis. | nine strains of streptococcus sanguis exhibited tolerance to benzylpenicillin: the growth of each strain was susceptible to penicillin with minimal inhibitory concentrations of 0.1 mug/ml or lower, but the bacteriolytic and bactericidal effects were limited in each case. the tolerance of these bacteria was also reflected in the large discrepancies between the minimal inhibitory and minimal bactericidal concentrations for benzylpenicillin. the hypothesis that a natural deficiency of endogenous mu ... | 1980 | 6104471 |
| chimeric streptococcal plasmids and their use as molecular cloning vehicles in streptococcus sanguis (challis). | chimeric plasmids, which were useful as cloning vehicles in a streptococcus sanguis (challis) host vector system, have been constructed. by using three different strategies of restriction endonuclease digestion and ligation, a deoxyribonucleic acid (dna) fragment bearing an erythromycin resistance determinant was ligated in vitro to a phenotypially cryptic plasmid from streptococcus ferus. recombinant plasmids could be recovered after transformation of s. sanguis (challis) with these preparation ... | 1980 | 6251030 |
| molecular cloning of an erythromycin resistance determinant in streptococci. | the erythromycin resistance determinant of plasmid pdb102, a derivative of plasmid psm19035, was cloned into the single hindiii site of the 3.6-megadalton cryptic streptococcus mutans plasmid pva318 and introduced into streptococcus sanguis strain challis by transformation. plasmid pdb201, which was isolated from one of the transformants, consisted of the vector plasmid and the 1.15-megadalton hindiii fragment d of psm19035. hindiii fragment d contained within it one of the two unique "spacer" s ... | 1980 | 6253443 |
| the effect of intensive antibacterial therapy on the sulcular environment in monkeys. part i. changes in the bacteriology of the gingival sulcus. | the changes induced in the bacteriology of the gingival sulcus were evaluated as part of a study considering the keratinizing potential of the sulcular epithelium when bacterial plaque was essentially eliminated. two rhesus monkeys were scaled and placed on a daily therapeutic regimen which included a prophylaxis, systemic tetracycline, and topical chlorhexidine. over the 40 day experimental period and 74 days post-therapy, subgingival plaque samples were taken periodically. the plaque samples w ... | 1980 | 6102117 |
| the use of monoclonal antibodies in the study of lactose-sensitive adherence of actinomyces viscosus t14v. | 1980 | 6108369 | |
| purification and properties of deoxyribonucleic acid binding factor isolated from the surface of streptococcus sanguis cells. | deoxyribonucleic acid (dna) binding factor (bf) was found in surface fluids from competent and noncompetent cells of streptococcus sanguis strains challis, wicky, and blackburn. fluids from noncompetent cells exhibited about 10% bf activity compared with extracts from competent cells. bf from competent wicky cells was purified to homogeneity by electrophoresis and immunodiffusion. purified bf preparations exhibited slight endonucleolytic activity, directed mainly against single-stranded dna. nuc ... | 1980 | 6245057 |
| effects of molecular weight of dextran on the adherence of streptococcus sanguis to damaged heart valves. | dextran-producing streptococci such as streptococcus sanguis are the organisms most frequently associated with infective endocarditis in humans. a series of experiments was designed to study how the molecular weight of dextrans affects the adherence of an endocarditis strain of s. sanguis to canine heart valves covered with platelets and fibrin. the data indicated that this adherence was dependent on dextrans of high molecular weight, such as dextran t-2000 or glucans isolated from s. sanguis or ... | 1980 | 6156909 |
| adherence of oral streptococci to keratinized and nonkeratinized human oral epithelial cells. | the ability of streptococcus mutans, streptococcus sanguis, streptococcus mitis, and streptococcus salivarius to adhere to keratinized versus nonkeratinized human oral epithelial cells was compared. s. mitis and s. salivarius exhibited significantly greater adherence to keratinized cells than to nonkeratinized cells. s. mutans and s. sanguis adhered equally well to either epithelial cell type. it is concluded that keratinization of epithelial cells may be a significant factor in the adherence of ... | 1980 | 6155336 |
| nutritionally deficient streptococcus: investigation of the hidden culprit in culture-negative endocarditis. | subacute bacterial endocarditis in a 72-year-old woman was found to be caused by nutritionally deficient ("satelliting") streptococcus sanguis. the organism, which required pyridoxal compounds or thiol substitutes, was identified after the application of relatively unusual but simple laboratory procedures. normally, the organism's nutritional requirements would be fulfilled by components of the human blood that are used to inoculate clinical blood cultures, provided that the dilutional effect of ... | 1980 | 6160620 |
| bacterial adherence in endocarditis: interaction of bacterial dextran, platelets, fibrin, and antibody. | 1980 | 6163197 | |
| rate-limiting steps of the glycolytic pathway in the oral bacteria streptococcus mutans and streptococcus sanguis and the influence of acidic ph on the glucose metabolism. | 1980 | 6249251 | |
| glucose and sucrose effects in the culture media on streptococcus sanguis omz 9 glucosyltransferase inhibition by anti-glucosyltransferase antibodies. | an anti-glucosyltransferase serum was prepared against a purified enzyme of streptococcus sanguis omz 9. antibody fractions obtained after gel filtration on a bio-gel p200 column were used to study enzyme-antibody interaction in broth culture containing either sucrose or glucose, and to localize ultrastructurally the enzyme on thin sections of bacteria using labelled and unlabelled antibodies. the antibody fractions had no effect on bacterial growth in broth culture. a strong inhibition of insol ... | 1980 | 6451617 |
| [discussion on the etiology of ulcerative colitis (uc)]. | 1980 | 6451867 | |
| interaction of inflammatory cells and oral microorganisms. ix. the bactericidal effects of human polymorphonuclear leukocytes on isolated plaque microorganisms. | 1980 | 6449581 | |
| iga1 proteases from haemophilus influenzae, streptococcus pneumoniae, neisseria meningitidis, and streptococcus sanguis: comparative immunochemical studies. | iga1 proteases from h. influenzae, n. meningitidis, s. pneumoniae, and s. sanguis were compared with respect to site of cleavage in the iga1 molecule and edta sensitivity. proteases from s. sanguis and s. pneumoniae cleaved the pro (227)-thr (228) bond within the hinge region of the alpha 1 chain and were inhibited by edta. h. influenzae iga1 protease cleaved the pro (231)-ser (232) peptide bond. the activity of iga1 proteases from h. influenzae and n. meningitidis was unaffected by edta. purifi ... | 1980 | 6768797 |
| nucleic acid transport driven by ion gradient across cell membrane. | 1980 | 6769706 | |
| conservation of cell wall peptidoglycan by strains of streptococcus mutans and streptococcus sanguis. | turnover of the cell wall peptidoglycan fraction of six different strains of streptococcus mutans and eight different strains of streptococcus sanguis was examined. cells were grown in the presence of [3h]lysine and [14c]leucine for at least eight generations and then chased in growth medium lacking the two labels. at intervals during the chase, samples of cultures were removed, and the amounts of the two labeled precursors remaining in the peptidoglycan and protein fractions were quantitated. s ... | 1980 | 6769821 |
| role of bacterial interactions in the colonization of oral surfaces of actinomyces viscosus. | the effects of several microbial residents of human oral cavities on the attachment of actinomyces viscosus t14v to tooth surfaces were assessed by using the saliva-coated hydroxyapatite (sha) in vitro system. attachment to sha by a. viscosus t14v was not inhibited in the presence of streptococcus mutans gs5, either in the presence or absence of sucrose. precoating sha beads with s. mutans in the presence of sucrose also did not retard strain t14v attachment. however, the presence of streptococc ... | 1980 | 6772577 |
| effect of temperature on the bactericidal action of sodium hypochlorite endodontic irrigant. | when the in vitro bactericidal action of 2.6 percent sodium hypochlorite endodontic irrigating solution was compared at room temperature (22 degrees c.) and at body temperature (37 degrees c.), sterility was achieved in significantly less time at 37 degrees c. | 1980 | 6779248 |
| effects of chlorhexidine and iodine on in vitro plaques of streptococcus mutans and streptococcus sanguis. | both chlorhexidine and iodine showed greater antimicrobial effect on in vitro grown s. mutans plaque than on plaque composed of s. sanguis. one treatment with iodine for 8 min inhibited the acid production of s. mutans plaque whereas s. sanguis plaque required 20 min to be similarly affected. in contrast one treatment with chlorhexidine for up to 20 min did not completely inhibit the acid production of plaque of either microorganism. repeated short term exposures increased the bactericidal effec ... | 1980 | 6929083 |
| hydrolysis of milk oligosaccharides by the oral bacterium streptococcus sanguis atcc 10557. | 1980 | 6931556 | |
| adherence of oral streptococci to hydroxyapatite in vitro via glycerol-teichoic acid. | 1980 | 6931558 | |
| switches in macromolecular synthesis during induction of competence for transformation of streptococcus sanguis. | the induction of synchronous development of competence for genetic transformation in streptococcus sanguis, by either endogenous or exogenous competence factor (cf), is manifested in the transient synthesis of a new set of at least 10 polypeptides, ranging from 14,000 to 51,000 in molecular weight. eight polypeptides (e14, e16, e24, e28, e32, e37, e44, e51) appear early, and two polypeptides (l34, l42) appear 5-10 min later. one of the newly synthesized early polypeptides, e16, is shown to be a ... | 1980 | 6934531 |
| electron microscopic mapping of deletions on a streptococcal plasmid carrying extraordinarily long inverted repeats. | deletions delta 101, delta 102, and delta 103 which occurred within the extraordinarily long inverted repeats of the self-ligated large ecori fragment of the streptococcal mls (macrolides, lincosamides, streptogramin b)-resistance plasmid psm19035 led to the formation of plasmids pdb101, pdb102, and pdb103. their molecular lengths were determined by contour length measurements to be 17.8, 17.4, and 13.9 kb, respectively. electron microscopic examination of self-annealed molecules revealed stem-l ... | 1980 | 6927813 |
| an in vitro model for studying adsorption of oral streptococci to crown and bridge cements. | a sensitive, in vitro method has been developed for the study of bacterial adsorption to dental crown and bridge cements. the method employs heavily radiolabelled cells which adhere to the wall in wells made from the cements. loss of radioactivity from the bacterial suspension during incubation is used to measure the degree of bacterial adherence to the materials. strains of streptococcus sanguis and streptococcus mutans adhered in greater numbers than a strain of streptococcus salivarius to bot ... | 1980 | 6928441 |
| effect of electrical potential on the adherence of streptococcus sanguis to gold plate. | 1980 | 6929822 | |
| in vitro development of chlorhexidine resistance in streptococcus sanguis and its transmissibility by genetic transformation. | three chlorhexidine-sensitive strains of streptococcus sanguis developed resistance to chlorhexidine when grown as continuous cultures in a fermenter containing medium with increasing concentrations of the drug. the mic of the test strains increased by between two and three dilution steps and these high values were maintained after daily transfer in drug-free medium for at least 15 d. in addition, the resistance of one of the strains persisted for more than 1 month of continuous growth in drug-c ... | 1980 | 6932090 |
| interaction of parotid saliva basic glycoprotein with streptococcus sanguis atcc 10557. | it is postulated that an initial step in dental plaque formation is the adherence of oral bacteria to the salivary pellicle. recently, we have found that a proline-rich and basic glycoprotein (mgp) from human parotid saliva, which is successfully purified by concanavalin a-sepharose affinity chromatography, binds to some oral streptococci such as s. mitis and s. sanguis. this paper deals with some inhibitors which affect the binding of the mgp to s. sanguis atcc 10557. the assay for the binding ... | 1980 | 6932503 |
| isolation of hemagglutinin from streptococcus sanguis atcc 10557. | 1980 | 6935255 | |
| establishment of potentially cariogenic streptococci in an experimental human plaque. i: streptococcus mutans. | streptococcus mutans (streptomycin resistant) was seeded to enamel slabs in the intraoral cariogenicity test (ict). after seven days in vivo, s. mutans comprised 58% of the total streptococci in the seeded plaque, compared to approximately 1% for the non-seeded control plaque. seeding s. mutans to the ict resulted in a change in the proportion of total streptococci in plaques, but no difference in ict caries compared to control slabs was recorded. | 1980 | 6937491 |
| microbiological, ultrastructural and spectroscopic analyses of the anti-tooth-plaque properties of fluoride compounds in vitro. | 1980 | 6944012 | |
| interactions of competent streptococcus sanguis (wicky) cells with native or denatured, homologous or heterologous deoxyribonucleic acids. | competent cell-deoxyribonucleic acid (dna) interactions were examined using tritium-labeled homologous or heterologous native or denatured dnas and competent streptococcus sanguis wicky cells (strain we4). the dnas used were extracted from we4 cells, escherichia coli b cells, and e. coli bacteriophages t2, t4, t6, and t7. the reactions examined were: (i) total dna binding, (ii) deoxyribonuclease-resistant dna binding, and (iii) the production of acid-soluble products from the dna. optimal temper ... | 1980 | 6991480 |
| identification of tetracycline-resistant r-plasmids in streptococcus agalactiae (group b). | in this report, 30 tetracycline-resistant clinical isolates of group b streptococcus were examined to assess the extent to which tetracycline resistance is plasmid mediated. of these, 27 showed no physical or genetic evidence of plasmid-mediated resistance; however, one conjugative and two small (3.5 x 10(6)-dalton) multicopy non-self-transmissible tetracycline resistance plasmids were identified. the conjugative plasmid was transmissible to streptococcus faecalis as well as to streptococcus aga ... | 1980 | 7004347 |
| aphthous ulcers--a review of the literature on etiology, pathogenesis, diagnosis, and treatment. | recurrent aphthous ulcerations are seen in everyday dental practice. these lesions may easily be misdiagnosed and mistreated. a comprehensive but nondefinitive review of the literature is presented in hope that the practitioner can better diagnose, evaluate, research, and treat ras. although many modalities of treatment have been advocated, a truly effective therapeutic regimen to prevent ras does not yet seem available. additional research on the ideology, pathogenesis, and treatment of ras is, ... | 1980 | 7005287 |
| isolation and immunobiological classification of streptococcus sanguis from human tooth surfaces. | a total of 113 pure cultures of streptococcus sanguis were obtained from dental plaque samples of 64 subjects. all isolates synthesized glucan from sucrose, elaborated peroxide, and were alpha-hemolytic. two biotypes and four serotypes were differentiated within the species. biotype a (95 isolates) fermented salicin and inulin and hydrolyzed arginine and esculin, whereas biotype b (18 isolates) did not possess these activities. the isolates were serotyped with autoclaved extracts against whole-c ... | 1980 | 7229009 |
| characterization of tufted streptococci isolated from the "corn cob" configuration of human dental plaque. | streptococci isolated from "corn cob" configurations of human dental plaque possess a polar fibrillar tuft extending 100 to 150 nm from one pole of the cell. the two strains studied were physiologically related to the streptococcus sanguis-streptococcus mitior group and were most similar to streptococcus mitis atcc 903. the corn cob streptococci were serologically related to s. sanguis serotype 1. the polar tuft contained at least two antigenically distinct components, one serologically related ... | 1980 | 6987171 |
| quantitative in vitro assay for "corncob" formation. | the interaction of bacterionema matruchotii with strains of streptococcus sanguis produces a structure which morphologically resembles a corncob. to determine the specific bacterial surface receptors involved in the interaction, we developed a quantitative assay. the assay consisted of mixing saline suspensions of [ch(3)-(3)h]thymidine-labeled streptococci and b. matruchotii, incubating at 37 degrees c for 2 h, and filtering the mixture through a 5-mum polycarbonate membrane filter. the free coc ... | 1980 | 7011981 |
| manganese antagonizes the inhibitory effect of fluoride on the glucose metabolism of streptococcus mutans. | manganese stimulated the utilization of glucose by streptococcus mutans, streptococcus sanguis, streptococcus mitior and streptococcus milleri. s. mutans serotype c strains formed larger amounts of intracellular polysaccharide, lactic acid and produced a lower terminal ph in glucose broth when grown in the presence of 0.5 mm mn. manganese stimulated the utilization of glucose by resting cell suspensions of each of the four oral streptococcal species examined. fluoride inhibited the utilization o ... | 1980 | 7242378 |
| simple method for demonstrating small plasmid deoxyribonucleic acid molecules in oral streptococci. | a simple procedure for rapidly demonstrating small plasmids (less than 10 megadaltons) in oral streptococci is described. logarithmic-phase, glycine-treated cells from 1.5-ml broth cultures were converted to osmotically fragile forms and lysed with sodium dodecyl sulfate. after the hydrodynamic shearing of host chromosomal deoxyribonucleic acid, such lysates were analyzed by low-voltage agarose gel electrophoresis. small plasmids, migrating significantly faster than chromosomal deoxyribonucleic ... | 1980 | 7396483 |
| genetic transformation of streptococcus sanguis (challis) with cryptic plasmids from streptococcus ferus. | by using the basic methodology initially published by kretschmer et al. (j. bacteriol. 124:225-231, 1975), we have been able to introduce phenotypically cryptic plasmids from streptococcus ferus (formerly streptococcus mutans subsp. ferus) into streptococcus sanguis by genetic transformation. in this system, the entry of the cryptic plasmids is selected indirectly. this is effected with transforming deoxyribonucleic acid mixtures in which the cryptic plasmid deoxyribonucleic acid is present in a ... | 1980 | 7399689 |
| lymphoproliferative responses to oral bacteria in humans with varying severities of periodontal disease. | we assessed in vitro the lymphocyte blastogenic resp onsess of peripheral blood lymphocytes to antigen extracts of a large battery of oral microorganisms in a population of humans with varying severities of periodontal disease. when the magnitudes and frequencies of statistically positive blastogenic responses to various oral microorganisms were compared, three general patterns emerged. the actinomyces species proved to be potent stimulators of lymphocyte blastogenesis in most subjects tested, w ... | 1980 | 7399694 |
| an immunoelectrophoretic analysis of the strep. sanguis and adult human oral mucosa antigen extracts used for immunological investigations of recurrent aphthous stomatitis. | water-soluble antigens of strep. sanguis strain 2a, atcc 10556, and adult human oral mucosa (ahom) were analysed by four immunoelectrophoretic methods. crossed immunoelectrophoresis (cie), using rabbit antibodies raised against the soluble antigens, revealed 26 antigens in the standard strep. sanguis antigen extract and 16 antigens in the standard adult human oral mucosa extract. all 26 strep. 2a antigen-antibody precipitates observed in the cie were identified as streptococcal components. eleve ... | 1980 | 7446131 |
| infective endocarditis, 1970-1979. a study of culture-positive cases in st. thomas' hospital. | ninety-three episodes of culture-positive endocarditis occurred in 88 patients in the years 1970 to 1979. streptococci caused two-thirds of the cases, with streptococcus mitior and streptococcus sanguis most common. among the group d streptococci, streptococcus bovis was much more common than streptococcus faecalis. staphylococci accounted for a quarter of the episodes. streptococcal endocarditis was commonest in the elderly, whereas staphylococcal endocarditis occurred at all ages. degenerative ... | 1980 | 7465764 |
| attachment of oral cytophaga species to hydroxyapatite-containing surfaces. | model systems simulating the cementum portion of teeth were used to characterize the attachment process by which certain species of oral cytophaga initiate the colonization of the tooth root surface in vitro. the adsorption of these bacteria to spheroidal hydroxyapatite beads and mechanically powdered root material followed langmuir isotherm kinetics. from such data, the number of binding sites per 20 mg of substrate and the affinity constants were evaluated for two strains of cytophaga sp. rest ... | 1980 | 7216436 |
| the deposition of streptococcus sanguis nctc 7868 from a flowing suspension. | measurements were made of the rates at which continuously cultured streptococcus sanguis nctc 7868 cells accumulated on the inside surfaces of narrow glass capillaries from suspensions of the bacteria flowing down the capillaries at different velocities. initially, the rate of accumulation of bacteria on the clean walls of the capillary was rapid. the deposition rate decreased with time, however, resulting in a saturation coverage of the glass surface which was considerably less than a monolayer ... | 1980 | 7229614 |
| presynaptic donor dna-protein complexes in transformation of streptococcus sanguis: identification of the protein component. | 1980 | 7378079 | |
| effects of monocytopenia and anticoagulation in experimental streptococcus sanguis endocarditis. | the role of blood monocytes in the attachment of streptococci to endocardial vegetations was investigated in an experimental streptococcus sanguis endocarditis by depletion of blood monocytes with the cytostatic drug vp 16-213 alone and combined with anticoagulant treatment with warfarin sodium. the numbers of streptococci in the vegetations of control, monocytopenic, and monocytopenic/anticoagulated rabbits were comparable. in the vegetations streptococci were found mainly in areas free of phag ... | 1980 | 7378272 |
| modulation of immunoreactivity to periodontal disease-associated microorganisms during pregnancy. | the lymphocyte blastogenic response to a panel of antigens and mitogens was assessed in a group of 20 women throughout their pregnancy. in addition, a group of five nonpregnant women was monitored simultaneously to identify variations in response to the same stimulants. the stimulants included orally associated bacterial antigens (streptococcus sanguis, actinomyces viscosus, bacteroides asaccharolyticus, bacteroides melaninogenicus subsp. intermedius, bacteroides [capnocytophaga] ochraceus, and ... | 1980 | 7399691 |
| microflora and chemical composition of dental plaque from subjects with hereditary fructose intolerance. | we compared the microbiological and chemical composition of dental plaque from subjects with hereditary fructose intolerance who restrict their dietary sugar intake with that of control subjects who do not. the two groups showed no significant differences in chemical composition of plaque: the mean protein, carbohydrate, calcium, magnesium, and phosphate contents were similar. dental plaque from both groups contained similar numbers of total colony-forming units per microgram of plaque protein, ... | 1980 | 7399699 |
| streptococcus sanguis sepsis and meningitis: a complication of vacuum extraction. | 1980 | 7416226 | |
| experimental streptococcus sanguis endocarditis: immune complexes and renal involvement. | an increase of agglutinins and circulating immune complexes (cic) was observed in the sera of rabbits after the induction of bacterial endocarditis (be) by intravenous injection of live streptococci into animals with a non-bacterial thrombotic endocarditis. rabbits immunized for 12 days with heat-killed streptococci developed higher levels of agglutinins and cic than did those unimmunized during be. the levels remained elevated in the immunized rabbits after the induction of streptococcal endoca ... | 1980 | 7418263 |
| bactericidal effect of hydrogen peroxide is prevented by the lactoperoxidase-thiocyanate system under anaerobic conditions. | streptococcus sanguis and peptostreptococcus anaerobius were exposed to various combinations of the components of the lactoperoxidase-thiocyanate-hydrogen peroxide system. the bactericidal effect of hydrogen peroxide was prevented under anaerobic conditions by lactoperoxidase together with thiocyanate, but not by lactoperoxidase or thiocyanate alone. thiocyanate was effective already at a molar ratio to hydrogen peroxide of 1:100. | 1980 | 7429633 |
| the effects of fluoride on the growth of oral streptococci. | four species of oral streptococci were examined for their ability to grow in a complex medium supplemented with naf, and at various ph levels. streptococcus mutans strains were the most susceptible to inhibition, with streptococcus mitior and streptococcus sanguis being the least affected. the s. mutans genotype comprising serotypes c, e and f was more susceptible to inhibition by fluoride than was the genotype comprising serotypes d and g. the results suggest that the fluoride concentration of ... | 1980 | 7432189 |
| ultrastructural location of streptococcus mutans and streptococcus sanguis antigens in carious human dentine. | the ultrastructural localization of bacterial antigens of streptococcus sanguis and of the various serotypes a,b,c,d,e,f and g of streptococcus mutans was studied in human carious dentine using the indirect peroxidase-antiperoxidase method with appropriate controls. no positive staining was seen in adjacent normal dentine. in the inner dentine underlying the cytoplasm of fibroblasts and schwann cells of unmyelinated nerve fibrils. in sclerosed tubules, or on the plasmalemma of the odontoblast pr ... | 1981 | 7035442 |
| vancomycin prophylaxis of experimental streptococcus sanguis. inhibition of bacterial adherence rather than bacterial killing. | using a strain of streptococcus sanguis tolerant to vancomycin to infect aortic vegetations in rats, we found that prophylactic intravenous vancomycin given 30 min before bacterial challenge decreased the incidence of endocarditis from 88 to 8% (p less than 10(-5)). because peak vancomycin serum levels were below the minimal bactericidal concentration, mechanisms of protection other than bacterial killing were investigated. s. sanguis were incubated with inhibitory concentration of vancomycin (5 ... | 1981 | 7287904 |
| constrictive pericarditis due to streptococcus sanguis. | we have described the third case of pericarditis caused by anaerobic streptococci in children. despite operative drainage and high-dose penicillin therapy, there was rapid progression to constrictive pericarditis. complete pericardiectomy was life-saving. | 1981 | 7221646 |
| bacterial aggregating activity in human saliva: comparisons of bacterial species and strains. | using a quantitative assay to measure saliva-mediated bacterial aggregating activity, we have surveyed 20 streptococcal strains with saliva samples obtained from a large population study. individual saliva samples demonstrated characteristic levels of aggregating activity for streptococcus sanguis m5. in general, high activity for this strain was associated with high activity for other strains of s. sanguis. streptococcus mitis, and streptococcus salivarius. the population distribution of aggreg ... | 1981 | 7228395 |
| adherence of streptococcus mutans and streptococcus sanguis to salivary components bound to glass. | adherence of radiolabeled streptococcus mutans and streptococcus sanguis to saliva-treated glass surfaces was studied under conditions which minimized bacteria-glass interactions. treatment of glass with an alkylsilane solution decreased nonspecific bacterial adherence and enhanced adsorption of radiolabeled salivary components to these surfaces. addition of triton x-100 to the bacterial suspensions also reduced nonspecific adherence to siliconized glass, but did not affect adherence to salivary ... | 1981 | 7251139 |
| genetic transformation of streptococcus mutans. | three strains of streptococcus mutans belonging to serotypes a, c, and f were transformed to streptomycin resistance by deoxyribonucleic acids derived from homologous and heterologous streptomycin-resistant strains of s. mutans and streptococcus sanguis strain challis. homologous transformation of s. mutans was less efficient than heterologous transformation by deoxyribonucleic acids from other strains of s. mutans. | 1981 | 7251168 |
| lactose-reversible coaggregation between oral actinomycetes and streptococcus sanguis. | freshly isolated strains of oral actinomycetes were obtained from human dental plaque and were tested for the ability to coaggregate with common laboratory stock strains of streptococcus sanguis. strains belonging to the genera actinomyces, arachnia, bifidobacterium, and bacterionema were isolated. only members of the genus actinomyces coaggregated with the streptococci, and only actinomyces viscosus and actinomyces naeslundii exhibited lactose-reversible interactions. a total of 61 strains, con ... | 1981 | 7263074 |
| microbial surface interactions: reduction of the haemagglutination activity of the oral bacterium fusobacterium nucleatum by absorption with streptococcus and bacteroides. | oral strains of fusobacterium nucleatum showed haemagglutination (ha) of sheep red blood cells and attachment of ha-active f. nucleatum fragments to other microorganisms allowed a means of studying microbial surface interactions. ha-active sonicated fragments (sf) prepared from f. nucleatum were mixed with whole cell suspensions of 48 bacterial strains and, after incubation, the whole cells were separated from the non-absorbed fragments by differential centrifugation. attachment of f. nucleatum ... | 1981 | 7041865 |
| expression of streptococcal plasmid-determined resistance to erythromycin and lincomycin in escherichia coli. | 1981 | 7035838 | |
| experimental endocarditis caused by streptococcus sanguis: single and combined antibiotic therapy. | the effectiveness of penicillin g, fosfomycin, and cefoxitin alone and in combination was studied in vitro and in the treatment of left-sided streptococcus sanguis endocarditis in rabbits. in vitro, the combinations penicillin g plus fosfomycin, penicillin g plus cefoxitin, and fosfomycin plus cefoxitin were synergistic or partially synergistic for s sanguis. therapy with the combinations was more effective in eradicating the species from cardiac vegetations that was that with each antibiotic us ... | 1981 | 7283409 |
| salivary immunoglobulin a antibodies reacting with antigens from oral streptococci: longitudinal study in humans. | the salivary immunoglobulin a (iga) activity to antigens from four common oral streptococci was analyzed in samples from five humans. from each individual, parotid and whole saliva were collected 12 times over a period of 4 months. in samples collected at different occasions, the salivary iga activity varied considerably. the variations showed a covariation with the concentration of total iga in the saliva samples. a covariation was also found between salivary iga antibodies of different specifi ... | 1981 | 7287178 |
| interference of secretory immunoglobulin a with sorption of oral bacteria to hydroxyapatite. | the potential of secretory immunoglobulin a (s-iga) to interfere with the initial phase of dental plaque formation was studied by using an in vitro method which permits the quantitative determination of the sorption of radiolabeled oral bacterial cells to hydroxyapatite (ha) beads. the importance of specific s-iga antibodies was evaluated by a comparison of the effect of pure preparations of colostral s-iga, polymeric myeloma iga, or preabsorbed s-iga. specific antibody molecules bound at the ha ... | 1981 | 7014466 |
| sucrose-induced ecological response of experimental dental plaques from caries-free and caries-susceptible human volunteers. | microbial succession, experimental cariogenicity, and sucrose metabolism were examined in dental plaques which developed on sterile bovine enamel inserts in acrylic palatal appliances. the appliances were worn for a period of 14 days by 10 caries-free and 10 caries-susceptible human volunteers. three of six enamel inserts on each appliance were exposed extraorally to 10% sucrose in 0.85% saline six times a day, and three were exposed simultaneously to 0.85% saline as a control environment. the r ... | 1981 | 7333665 |
| enolases from fluoride-sensitive and fluoride-resistant streptococci. | the enolase from a highly fluoride-sensitive strain of streptococcus salivarius and its fluoride-resistant mutant, as well as those from strains of streptococcus sanguis and streptococcus mutans with intermediate and low sensitivities to fluoride have been shown to be inhibited by fluoride. comparisons of the purified, strain-specific enzymes showed a high degree of similarity for all preparations. the michaelis constants for the substrate 2-phosphoglycerate were 1.3 x 10(-4) to 2.4 x 10(-4) m, ... | 1981 | 7333671 |
| viability of six species of normal oropharyngeal bacteria after exposure to cigarette smoke in vitro. | bacteria in culture medium at 37 degrees c were subjected to eight puffs of smoke from one cigarette, and viable cell counts were done at intervals ranging from 0 min to 3 h after exposure to smoke. the cigarette contained 23.0 mg of tar and 1.4 mg of nicotine. each 25.0 ml puff of smoke from a mechanical smoking apparatus was passed over a 1.0 ml suspension of bacteria dispersed over a 25 cm2 surface in a sterile flask. filtered air was in contact with the suspension between puffs, and during t ... | 1981 | 7339446 |
| role of bacteriocin during plaque formation by streptococcus salivarius and streptococcus sanguis on a tooth in an artificial mouth. | 1981 | 7251515 | |
| genetic transformation in streptococcus sanguis. competence factor and competence factor inactivator. | genetic transformation in streptococcus sanguis is a complex, multi-step process, involving several factors. the competence factor (cf), occurring in the culture filtrates of some strains, is essential in streptococcal transformation, but other factors are also involved. the presence of serum during growth is not obligatory for the preparation of active culture filtrate, but serum increases the amount and duration of cf activity of some strains. the stability of the cf activity differs distinctl ... | 1981 | 7257776 |
| genetic transformation of streptococcus sanguis. further studies on the production and isolation of the competence factor. | two new media were developed, containing only the dialyzable components of todd hewitt broth (th) with (medium ii) or without (medium iv) inactivated horse serum. the two media were used to detect activity of the competence factor (cf) and the competence factor inactivator (cgi) of streptococcus sanguis, and in preliminary experiments of cf isolation. because all the dissolved substances with molecular weight (mol. wt.) less than 12,000 can be removed from these media by dialysis, leaving the cf ... | 1981 | 7257777 |
| microbial studies on plaque from carious and caries-free proximal tooth surfaces in a population with high caries experience. | 1981 | 6973394 | |
| pulmonary clearance and phagocytic cell response to normal pharyngeal flora. | because human lungs are repetitively inoculated with the normal bacterial flora of the pharynx, we determined the pulmonary clearance of representative species after aerosol inoculation of a murine model, and characterized the phagocytic cell response by bronchoalveolar lavage. viable bacteria remaining in the lungs at 1, 2, and 4 h were: streptococcus sanguis, 24%, 8%, and 1%; streptococcus salivarius, 49%, 24%, and 5%; neisseria catarrhalis, 69%, 49%, and 22%. clearance of streptococcus sangui ... | 1981 | 7235361 |
| role of streptococcus sanguis (strain wicky) cell surface-located deoxyribonucleic acid-binding factor in transformation of a homologous strain. | in a previous report we demonstrated the presence of a factor binding deoxyribonucleic acid (dna) in vitro (bf) in cell leakage fluids from transformable streptococcus sanguis strains wicky, challis, and blackburn. bf originating from strain wicky was purified to homogeneity, and its properties are described. in this work, it was found that bf occurs at the surface of wicky cells in two forms, loosely bound (lb-bf) and strongly bound to the cell envelope. it was demonstrated that lb-bf formed fa ... | 1981 | 6894295 |
| effect of bacterial aggregation on the adherence of oral streptococci to hydroxyapatite. | several in vitro assay systems to measure the adherence of human dental plaque bacteria to solid surfaces such as teeth, glass, and hydroxyapatite have been published. in many studies a variety of macromolecular solutes have been used to study the adherence process. often these solutes are able to aggregate the test bacterial and thus may alter the outcome of adherence experiments. in this study, the effects of the aggregation of streptococcus sanguis on adherence to spheroidal hydroxyapatite is ... | 1981 | 7228408 |