Publications

effect of metoclopramide on adrenal secretion in sheep: influence of dexamethasone and sodium intake.	metoclopramide, a competitive dopamine antagonist, stimulates aldosterone in man and monkey without affecting cortisol secretion. in sheep, metoclopramide also stimulates aldosterone but ist action on adrenocortical secretion is more controversial. to clarify the action of metoclopramide in conscious sheep, the response of plasma aldosterone, cortisol, angiotensin ii and potassium were studied after 0.16 and 0.64 mg/kg metoclopramide, with and without pretreatment with dexamethasone. the effect ...	1987	3613572
effect of metoclopramide on adrenal secretion in sheep: influence of dexamethasone and sodium intake.	metoclopramide, a competitive dopamine antagonist, stimulates aldosterone in man and monkey without affecting cortisol secretion. in sheep, metoclopramide also stimulates aldosterone but ist action on adrenocortical secretion is more controversial. to clarify the action of metoclopramide in conscious sheep, the response of plasma aldosterone, cortisol, angiotensin ii and potassium were studied after 0.16 and 0.64 mg/kg metoclopramide, with and without pretreatment with dexamethasone. the effect ...	1987	3613572
effects of roll tilt of the animal and neck rotation on different size vestibulospinal neurons in decerebrate cats with the cerebellum intact.	in decerebrate cats with the cerebellum intact we recorded the activity of lateral vestibulospinal neurons projecting to lumbosacral segments of the spinal cord (ivs neurons) and related the resting discharge, as well as the response characteristics of these neurons to roll tilt of the animal and neck rotation, with the cell size inferred from the conduction velocity of the corresponding axons. a slight negative correlation was found between resting discharge rate and conduction velocity of the ...	1987	3615170
in vitro antistaphylococcal activity of pefloxacin alone and in combination with other antistaphylococcal drugs.	mics of pefloxacin and nine antistaphylococcal drugs were determined for 200 isolates of staphylococcus aureus, staphylococcus epidermidis, staphylococcus haemolyticus, staphylococcus hominis, and staphylococcus saprophyticus. all the strains were susceptible to pefloxacin, vancomycin, and rifampin. oxacillin-resistant strains were uniformly resistant to cephalothin and were more likely to be resistant to gentamicin, erythromycin, clindamycin, doxycycline, and trimethoprim-sulfamethoxazole than ...	1987	3481244
biphasic effects of epidermal growth factor on bone nodule formation by isolated rat calvaria cells in vitro.	osteoblast-like cells isolated from fetal rat calvaria (rc) form three-dimensional nodules in vitro having the morphological and immunohistochemical characteristics of bone. when administered continually over 21 days of culture, egf caused a dose-related inhibition of nodule formation, as well as a reduction in the proportion of mineralized nodules, at concentrations between 10(-12) to 10(-7) m. the same conditions caused an increase in both cell proliferation and saturation density in the cultu ...	1987	3502681
biphasic effects of epidermal growth factor on bone nodule formation by isolated rat calvaria cells in vitro.	osteoblast-like cells isolated from fetal rat calvaria (rc) form three-dimensional nodules in vitro having the morphological and immunohistochemical characteristics of bone. when administered continually over 21 days of culture, egf caused a dose-related inhibition of nodule formation, as well as a reduction in the proportion of mineralized nodules, at concentrations between 10(-12) to 10(-7) m. the same conditions caused an increase in both cell proliferation and saturation density in the cultu ...	1987	3502681
fluorescent low density lipoprotein and acetylated low density lipoprotein labeling of cultured bovine trabecular endothelial cells.		1987	3508231
[topical organization of the neuronal projections of the substantia nigra and ventral field of the tectum mesencephali onto the head of the caudate nucleus in the cat brain].	the investigation performed on cats by means of retrograde axonal transport of horseradish peroxidase and luminophores has presented the data demonstrating spatial organization of separate part projections of the nigral complex and the tegmental ventral field to various segments of the caudate nucleus head. terminal fields from neurons of various parts of the substantia nigra and the tegmental ventral field are demonstrated to overlap in segments of the caudate nucleus. experiments with double f ...	1987	3426407
experimental study of autologous free omental grafts.	the fate of intraabdominal free omental grafts was studied in 15 dogs and it was found that a connection between the hollow organ and the transplanted omentum was established only when the former had been injured. the larger, pigeon-egg-sized free fatty omental grafts became necrotic already from the fourth day onwards being replaced by an abscess-like structure, while in thin membrane-like grafts tissue necrosis due to the creeping in of connective tissue was less marked. in both cases, an inta ...	1987	3434086
molecular epidemiology of trimethoprim resistance among coagulase-negative staphylococci.	a 42% (70 of 167 isolates) incidence of resistance to 20 micrograms of trimethoprim per ml was found among clinical isolates of coagulase-negative staphylococci from two hospitals. a specific trimethoprim resistance gene probe from a conjugative staphylococcus aereus plasmid was used to investigate the location of the trimethoprim resistance gene among 29 isolates. in 14 trimethoprim-resistant isolates, the probe hybridized with only chromosomal dna, in 9 it hybridized with only plasmid dna, and ...	1987	3435115
phenotypic expression and genetic heterogeneity of lincosamide inactivation in staphylococcus spp.	we examined the resistance phenotype and the genetic basis of lincosamide modification in 25 clinical isolates of staphylococcus spp. inactivating lincomycin and clindamycin. the strains were resistant to high levels of lincomycin but remained susceptible to clindamycin. however, mbcs and inoculum effects showed that the activity of clindamycin was impaired. the distribution in these strains of nucleotide sequences related to lina and lina', the genes encoding lincosamide nucleotidylation in sta ...	1987	3439797
expression of tumor cell properties in synovial cells in culture.	the tumorigenic properties of human rheumatoid arthritis synovial cells in culture were investigated. the synovial cells developed good colonies and secreted plasminogen activator (pa) and collagenase in the cell cultures, as do hela cells. since pa and progesterone receptor (pgr) are considered to be end products of estradiol action in breast cancer cells, the estrogen receptor (er) and pgr content in these cells was also assayed. large amounts of er and pgr were detected in the synovial cells ...	1987	3028023
hirano body filaments contain actin and actin-associated proteins.	hirano bodies are eosinophilic, rod-shaped intraneuronal inclusions whose frequency increases with age and with alzheimer's disease. to investigate their composition and possible relationship to the neuronal cytoskeleton, we employed immunocytochemistry and immunoelectronmicroscopy by using antisera to cytoskeletal proteins. the presence of actin, alpha-actinin, vinculin and tropomyosin was demonstrated diffusely throughout the hirano body. the presence of these proteins supports the contention ...	1987	3029338
ligand- and weak base-induced redistribution of asialoglycoprotein receptors in hepatoma cells.	the receptor for asialoglycoproteins (asgpr) was localized in human hepatoma hep g2 cells by means of quantitative immunoelectron microscopy. without ligand added to the culture medium, we found 34% of the total cellular receptors on the plasma membrane, 37% in compartment of uncoupling receptor and ligand (curl), and 21% in a trans-golgi reticulum (tgr) that was defined by the presence of albumin after immuno-double labeling. a small percent of the asgpr was associated with coated pits, the gol ...	1987	3034918
comparison of four serological tests for the detection of specific immunoglobulin m in cord sera of infants congenitally infected with cytomegalovirus.	four serological tests (3 immunoassays using enzyme-labelled antigen and 1 radioimmunoassay) were compared as regards the detection of cytomegalovirus (cmv) immunoglobulin m in cord sera. 68 cord sera from infants congenitally infected by cmv were included in the study. the infections were primarily diagnosed by virus isolation close to birth. four laboratories in 3 countries were involved, each laboratory using its own or a commercial test. of the sera tested in the different laboratories 50-80 ...	1987	3039652
temporal requirement for epidermal growth factor and insulin in the stimulation of hepatocyte dna synthesis.	primary monolayer cultures of adult rat hepatocytes were used to study the temporal interaction of epidermal growth factor (egf) and insulin in their stimulation of dna synthesis. the hepatocytes were cultured both under defined conditions and with serum. egf and insulin interacted synergistically. the entry into s phase (g1 exit) followed first-order kinetics both in untreated and hormone-stimulated cells. addition of egf and insulin at the time of plating did not alter the lag period before th ...	1987	3294862
campylobacter pyloridis: a new organism to explain an old problem?		1987	3309916
[medico-genetic studies of human provisional organs].		1987	3318529
effect of cyclooxygenase blockade on gas exchange and hemodynamics in pseudomonas pneumonia.	acute bilateral pseudomonas aeruginosa pneumonia was induced in 10 anesthetized dogs, after which five dogs received intravenous indomethacin (2 mg/kg) (indomethacin group), whereas five others were infused with saline (2 ml/kg) (control group). plasma levels of 6-ketoprostaglandin f1 alpha(6-keto-pgf1 alpha) and thromboxane b2 (txb2), stable metabolites of prostacyclin (pgi2) and thromboxane a2 (txa2), respectively, were measured by radioimmunoassay. although txb2 levels were not different befo ...	1987	3320011
biosynthesis of 1-alkenes in higher plants. a model study with the composite carthamus tinctorius l.	uneven numbered 1-alkenes, such as 1-pentadecene, 1-heptadecene, 1,8-heptadecadiene, 1,8,11-heptadecatriene and 1,8,11,14-heptadecatetraene are the predominant hydrocarbons in germinating safflower (carthamus tinctorius l.). according to their chain length and positions of double bonds they are derived from palmitic, stearic, oleic, linoleic or linolenic acid, respectively. this has been proved by administration of synthetic alpha-, beta-, or gamma-deuterated 12-phenyldodecanoic acids to germina ...	1987	3816783
[dangerous refuse].		1987	260384
evaluation of endodontic instruments as received from the manufacturer: the demand for quality control.	two hundred seventy new files, reamers, and hedström files from three different manufacturers were evaluated for debris and defects. statistical analysis of the new instruments showed that one company's instruments were cleaner than the other two but still contained defects. a standard for quality is proposed.	1987	269342
the general practitioner and continuing education.	a continuing education program for the general practitioner is presented that would provide a degree or certificate of advanced status on completion. this long-term program would be made possible by giving credit hours for continuing education courses taken, then redeeming these credits for the certificate or degree on completion. the dentist's choice in continuing education programs is protected because he can choose between programs with or without objectives, and programs that are tailored to ...	1988	266520
toxic effects of natural saline waters on mallard ducklings.	water from 10 saline wetlands in saskatchewan was provided as drinking water for 1-day-old mallards (anas platyrhynchos). ducklings given water with conductivity from 3,750 to 7,490 microns hos/cm grew as well as birds on fresh water during a 14-day trial, but birds given water with conductivity of 4,000 microns hos/cm grew poorly during the last 2 wk of a 28-day trial. ducklings given water with conductivity of 7,720 micron hos/cm grew poorly during a 14-day trial. six of 10 ducklings given wat ...	1988	3352095
protective effect of glucan against experimentally induced staphylococcal mastitis in ewes.	glucan, an immunostimulant, was evaluated for its ability to modify a staphylococcal mammary challenge in ewes. glucan was administered subcutaneously to ewes prior to lactation or during lactation, and all ewes, including a control group, were subsequently challenged intramammarily with staphylococcus haemolyticus 40 days after the mean lambing date. the glucan treatment was shown to modify the staphylococcal mammary infection as the milk bacterial counts from all of the glucan-treated groups w ...	1988	3354193
piezoelectric polymer curvature sensor for measurement of regional curvature radius of lv wall.	to evaluate regional myocardial function, we developed a curvature sensor for direct and instantaneous measurement of the regional curvature radius of the lv wall. the sensor is a bimorph of two sheets of thin piezoelectric polymer film. the relation between output voltage of the sensor and the reciprocal of the known curvature radius has been shown to be linear. in anesthetized dogs, we inserted the curvature sensor into the subepimyocardium with a specially designed introducer. special care wa ...	1988	3364584
effects of estradiol on cerebral cortical neurons and their responses to adenosine.	the effects of iontophoretically applied 17 alpha- and 17 beta-estradiol on the spontaneous firing of, and actions of purines (adenosine, adenosine 5'-n-ethylcarboxamide) on, neurons in the rat cerebral cortex have been determined. both steroids, applied as acetate, sulphate or hemisuccinate esters, depressed the spontaneous firing of approximately half of the neurons tested. 17 beta-estradiol potentiated the inhibitory actions of adenosine on some neurons (26%) and antagonized the effects of ad ...	1988	3370499
subacute bacterial endocarditis in a patient with ankylosing spondylitis.	a 57 year old white man with aortic insufficiency and previously undiagnosed ankylosing spondylitis, who developed subacute bacterial endocarditis (sbe), is described. emergency aortic valve replacement was necessary, and the aortic valve pathology showed diffusely fibrosed and thickened valve leaflets with bacterial vegetations. this is the first recorded case of sbe in ankylosing spondylitis.	1988	3389930
new orally active alpha-ketohydroxy pyridine chelators for the treatment of iron overload.		1988	3390580
influence of coenzyme q10 on doxorubicin uptake and metabolism by mouse myocardial cells in culture.		1988	3416371
genetic characterization of leishmania isolates at 37 enzyme loci.		1988	3417374
experimental evaluation of the mitroflow pericardial heart valve prosthesis. part ii. pathologic examination.	the results of the morphologic and histopathologic examination of 37 bovine pericardial heart valve prostheses that were retrieved after experimental implantation in chacma baboons for periods of one to twelve months are presented in this study. the implanted prostheses consisted of 33 mitroflow valves, size 21 mm, that belonged to four different groups according to the method of preparation of the pericardium (process i to iv) and 4 commercially available ionescu-shiley valves, size 19 mm. all ...	1988	3421507
the establishment and characterization of mouse l-929 cells in protein-free eagle's minimal essential medium.	the mouse cell line l-929 was established in protein-free eagle's minimal essential medium. the cells have been 'adapted' to continuous growth in the medium using stepwise reductions in the concentration of fetal bovine serum. the cells designated l-929-ws have now been propagated in protein-free eagle's minimal essential medium for two years. the population-doubling time was about 37 h. the addition of serum stimulated cell growth only slightly, but the saturation density was significantly incr ...	1988	3058707
quality analysis of dsa equipment.	in the framework of a quality analysis project for the improvement of digital subtraction angiography (dsa) equipment, an inventory was made of the image quality and radiation dose of dsa equipments in six hospitals in the netherlands. the image quality was investigated with a contrast detail (cd) phantom. the entrance dose of the radiation on this phantom and the skin dose at the level of the eye lenses and the thyroid gland were measured in these hospitals using a human phantom during a standa ...	1988	3067118
the bacteria responsible for ureolysis in artificial dental plaque.	the origin of ureolytic activity in artificial-mouth plaques was established by assessing the contribution to plaque ureolytic activity of the isolated bacteria. to overcome losses of ureolytic activity caused by the unstable presence of urease in oral bacteria, ureolytic bacteria were isolated from an exceptionally active plaque (1 mumol nh3/min per mg protein) in which 63 per cent of the flora was ureolytic. after their ability to metabolize urea was stabilized, 13 ureolytic bacteria remained: ...	1988	3075450
blast colonies containing hemopoietic progenitor cells can give rise to abelson virus (a-mulv)-transformed cell lines.	we have established an in vitro system with which to examine the ability of abelson murine leukemia virus (a-mulv) to infect early hemopoietic progenitor cells. blast cell colonies containing less than 100 cells were shown to contain up to 85% of cells with secondary hemopoietic colony-forming ability. infection of cells from these blast colonies resulted in generation of transformed mast cell lines when a feeder was provided. morphological examination of cells taken from infected cultures at va ...	1988	2446900
activation of the mas oncogene during transfection of monoblastic cell line dna.	using the tumorigenicity assay, we observed an activation of the mas oncogene during transfection of human acute myelocytic leukemia (ctv-2) dna. respective transfectants contained amplified mas sequences characterized by rearrangements in 5' and 3' noncoding regions that were transcribed into a 3.3 kb mas rna species. our data represent the second report on activation of the mas gene.	1988	2453759
activation of vesicular stomatitis virus fusion with cells by pretreatment at low ph.	fusion of vesicular stomatitis virus (vsv) with vero cells was measured after exposure of the virus to low ph under a variety of experimental conditions. the method of relief of fluorescence self-quenching of the probe octadecylrhodamine was used to monitor fusion. incubation of the virus at ph 5.5 prior to binding to cells led to significant enhancement of fusion at the plasma membrane, whereas fusion via the endocytic pathway was inhibited. fusion of ph 5.5-pretreated vsv showed a similar ph t ...	1988	2832405
high-performance liquid chromatographic separation and immunological characterization of soluble bovine viral diarrhea virus antigen.	sodium dodecyl sulphate-polyacrylamide gel electrophoresis and western blot analysis of the protein extracts from bovine viral diarrhea virus (bvdv, singer strain) infected primary calf testicle cells (soluble antigen) showed the presence of four virus specific polypeptides of 105, 90, 84 and 67 kilodaltons (kd) the 84-kd being the most abundant. anion-exchange high-performance liquid chromatography (hplc) of soluble antigen separated the virus specific polypeptides in individual peaks while the ...	1988	2832429
opioids and behavior: genetic aspects.	three animal models, based on genetic differences in endogenous opioid peptides and opioid receptors, are described. obese mice and rats, whose pituitary opioid content is elevated, may be used to investigate eating disorders. recombinant inbred strains of mice, which differ in brain opioid receptors and analgesic responsiveness, can be used for study of opioid- and nonopioid-mediated mechanisms of pain inhibition. individual reactivity to opioids can be examined in c57bl/6 and dba/2 inbred stra ...	1988	2837410
inactivation of lincosaminide antibiotics in staphylococcus. identification of lincosaminide o-nucleotidyltransferases and comparison of the corresponding resistance genes.	resistance to lincomycin by inactivation has been detected in numerous clinical isolates of staphylococcus; in crude extracts of staphylococcus haemolyticus bm4610 and staphylococcus aureus bm4611, inactivation of lincomycin and clindamycin requires the presence of a nucleoside 5'-triphosphate (atp, gtp, ctp, or utp) as nucleotidyl donor and mg2+ as cofactor. the biochemical mechanism of lincosaminide inactivation was elucidated by determination of the structure of inactivated lincomycin and cli ...	1988	2846528
characteristics of coagulase-negative staphylococci that help differentiate these species and other members of the family micrococcaceae.	one hundred reference strains and 1,240 clinical isolates representing 26 species of the family micrococcaceae were used to evaluate the potential of tests for synergistic hemolysis, adherence to glass, pyroglutamyl-beta-naphthylamide hydrolysis, and susceptibility to a set of five antimicrobial agents for differentiating these species and strains within the species. sixty-eight percent of the clinical isolates exhibited synergistic hemolysis; 69% of the clinical staphylococci but none of the mi ...	1988	2846632
influence of immunomodulatory agents on bovine humoral and cellular immune responses to parenteral inoculation with bovine rotavirus vaccines.	sodium diethyldithiocarbamate (dtc), mycobacterium cell wall extract (mcwe, regressin), killed corynebacterium parvum (c. parvum, immunoregulin) and muramyldipeptide (mdp) were each combined with purified, live bovine rotavirus and inoculated into 3 month-old holstein-friesian calves in order to examine their ability to potentiate specific humoral and cellular immune responses. the vaccinated calves were boosted twice at 3 and 6 weeks after initial vaccine inoculation. the rotavirus was administ ...	1988	2847397
in-vitro induction of resistance in coagulase-negative staphylococci to vancomycin and teicoplanin.	twenty strains of coagulase-negative staphylococci (18 strains of staphylococcus haemolyticus and two of s. epidermidis) were serially subcultured in broth media containing subinhibitory concentrations (half the mic) of either vancomycin or teicoplanin. the mic of the antibiotic was again measured after five passages in antibiotic-containing broth. the organisms were then subcultured in broth containing antibiotic concentrations half of the new mic value. the experiment was terminated after 25 p ...	1988	2972670
glutamine synthetase: a marker of an astroglial subpopulation in primary cultures of defined brain areas.	primary cultures from various areas of newborn mouse brain were developed and characterized. enriched astroglial cultures of the cerebral hemispheres, cerebellum, medulla oblongata and olfactory bulbs contained about 80-90% glial fibrillary acidic protein (gfa) immunolabelled cells. these cultures were composed of a majority of flat, 'protoplasmic-like' cells. the aim of this culture model was used: (1) to study glutamine synthetase (gs) activity during in vitro astroglial development; (2) to co ...	1988	2897279
mutants of actinomyces viscosus t14v lacking type 1, type 2, or both types of fimbriae.	mutants of actinomyces viscosus t14v lacking type 1 or type 2 fimbriae or both were selected by their failure to react with rabbit antibodies against either or both fimbrial antigens. immunospecific double labeling with iron dextran and ferritin-conjugated antibodies showed two types of fimbriae on individual cells of the parent organism, a single type on mutant strains with type 1+2- and type 1-2+ fimbriae and no labeled or unlabeled fimbriae on a type 1-2- fimbria-deficient strain. the mutatio ...	1988	2902012
localization of the mdx mutation within the mouse dystrophin gene.	we have mapped human and mouse x chromosome-specific genomic and cdna probes through an interspecies mus musculus/spretus pedigree which contains the mdx mutation. the positions of these markers relative to one another and to the mdx mutation were delineated. using probes corresponding to segments of the human duchenne muscular dystrophy (dmd) gene transcript, the position of a cross-hybridizing mouse equivalent gene (mdmd) was located. in more than 200 animals mapped, three were identified whic ...	1988	2903046
site of action of a gonococcal growth inhibitor produced by staphylococcus haemolyticus.	the inhibitory substance produced by staphylococcus haemolyticus strain no. 7 acts on growing as well as resting gonococcal cells, as shown by reductions in viable counts. the optical density of these cell suspensions was only slightly reduced. the inhibitor caused lysis of gonococcal spheroplasts at 24 degrees c and 37 degrees c, but was much less active at 4 degrees c. acting on intact gonococcal cells, the inhibitor caused a temperature-dependent release of radioactive cytoplasmic material. e ...	1988	3134553
the amino acid sequence of a gonococcal growth inhibitor from staphylococcus haemolyticus.	a gonococcal inhibitor produced by staphylococcus haemolyticus was separated into three components by reverse-phase h.p.l.c. the amino acid composition analysis of each of the three components indicated extensive similarities. n-terminal sequence analysis of all three components allowed the identification of the first 27-30 residues of each. the complete primary structure of each component was determined from the sequence analysis of trypic peptides and peptides generated by mild acid hydrolysis ...	1988	3138972
rat catcher's warfarin treatment associated with rectal haemorrhage.		1988	3147777
detection of parasite antigens in different biological fluids.		1988	3151006
transferrin receptor and 4f2 expression by nk-sensitive and nk-resistant tumour cell lines.	upon stimulation with a phorbol ester and known tumour promoter, 12-o-tetradecanoylphorbol-13-acetate (tpa), the human erythroleukemia k562 cell line, a standard target for human natural killer (nk) cells, shows a significant reduction in expression of transferrin receptors (tfr) and becomes resistant to nk-mediated cytolysis. prompted by the initial finding that expression of tfrs by k562 cells correlates with target sensitivity to nk cytotoxicity, we have investigated the role of two proposed ...	1988	3165309
trisomy 11 in nonlymphocytic neoplasia.		1988	3165669
suppression of the radiation-induced expression of a tumor-associated antigen in human cell hybrids by the protease inhibitor antipain.	the effect of the protease inhibitor antipain (1.25 micrograms/ml) on the radiation-induced expression of a tumor-associated antigen in human cell hybrids has been investigated. a variety of treatment protocols have been studied where antipain was present before, during and at various times post-irradiation. it was found that antipain suppressed the radiation-induced expression of the tumor-associated antigen in all treatment protocols. the most effective suppression was obtained in those protoc ...	1988	3191580
[echocardiographic assessment of the functional state of an experimental aorto-left ventricular conduit].		1988	3209092
immunostimulating staphylococcal lipoteichoic acid prevents pulmonary tumor colonization in balb/c-mice.	immunostimulating and antineoplastic activities of staphylococcal lipoteichoic acid (lta) were studied in balb/c-mice. systemic administration of lta (1 mg or 2 mg i. p., 7 and 4 days prior to challenge) significantly enhanced chemiluminescence response of peritoneal macrophages (p less than 0.0125) and induced enlargement of the spleen (p less than 0.025) as compared to non-treated controls. in vivo the number of lung colonies was significantly lower (p less than 0.0125) in lta-treated mice 14 ...	1988	3223138
[kidney morphology in hemolytic-uremic syndrome].	morphology of the kidneys in hemolytic-uremic syndrome is considered basing on autopsy findings obtained for 3 infants with 5-17-day history of acute renal failure. a newborn infant of 17 days developed the disease after feto-fetal hemotransfusion when macerated fetus-donor hemolysis products entered the circulation of the fetus-recipient through monochorionic placenta. the second case in an infant of 6 months was due to adtp vaccine. the last infant aged 16 months manifested the syndrome in the ...	1988	3233069
periodicity coding in the inferior colliculus of the cat. ii. topographical organization.	1. the topographical distributions of single-unit and multiple-unit responses to amplitude-modulated tones--and to other relevant parameters of simple tonal stimuli--were defined across the main frequency representational gradient and within narrow frequency ranges represented in "frequency band laminae" in the principal midbrain auditory nucleus, the central nucleus of the inferior colliculus (icc), in adult, barbiturate-anesthetized cats. 2. responses to amplitude-modulated tones with the carr ...	1988	3236053
periodicity coding in the inferior colliculus of the cat. ii. topographical organization.	1. the topographical distributions of single-unit and multiple-unit responses to amplitude-modulated tones--and to other relevant parameters of simple tonal stimuli--were defined across the main frequency representational gradient and within narrow frequency ranges represented in "frequency band laminae" in the principal midbrain auditory nucleus, the central nucleus of the inferior colliculus (icc), in adult, barbiturate-anesthetized cats. 2. responses to amplitude-modulated tones with the carr ...	1988	3236053
staphylococcus haemolyticus: cocarde growth around imipenem disks.	twelve multiple-drug-resistant isolates of coagulase-negative staphylococci from diverse clinical sources, which had yielded cocarde growths (double zones of inhibition) around 10 micrograms imipenem disks, were identified as staphylococcus haemolyticus. this was the only staphylococcal species which exhibited this unusual phenotypic trait at our institution during a 7-month observation period.	1988	3243092
studies on type ii progesterone receptor in mcf-7 cells.	these experiments demonstrate for the first time the existence of a type ii progesterone receptor (rpii) in mcf-7 human breast cancer cells. rpii was shown to have a lower affinity for tritiated progesterone ([3h]pg) (kd greater than or equal to 13 nm) than classical rp (kd less than or equal to 3 nm). rpii was detected by cytosolic, nuclear, and whole cell assays of mcf-7 cells. scatchard analysis of [3h]pg binding data revealed that classical rp but not rpii could be recompartmentalized from t ...	1988	3254626
functionally polarized layers formed by epidermal cells on a permeable transparent collagen film.	rat epidermal cells were cultured on a transparent collagen film, which was permeable to low mr substances. then the cells were bathed in both media (apical and basal), which were separated by the collagen membrane. the cells formed a multi-layered epidermal sheet with well-developed structures of desmosomes. this sheet on a permeable support was found to be an effective permeability barrier for glucose and amino acids. the epidermal layer showed functional polarity for the uptake and excretion ...	1988	3255753
maintenance of multiplication rate stability by cell populations in the face of heterogeneity among individual cells.	we addressed the question of how a population of cells can maintain a constant multiplication rate given the heterogeneity of its individual members. using the nih 3t3 line of mouse embryo cells, comparisons were made of multiplication rates between offspring subclones and the parental clones from which the subclones were derived. parents at all levels of multiplication rate produced offspring with a wide range of multiplication rates. offspring from parent cells with the highest growth rates ra ...	1988	3255757
staphylococcus haemolyticus urinary tract infection in a male patient.	urinary tract infections caused by staphylococci are usually attributed to staphylococcus epidermidis or s. saprophyticus. the case study reported here describes a persistent urinary tract infection caused by s. haemolyticus in a 38-year-old male whose infection was ultimately resolved through the use of the antibiotic trimethoprim-sulfamethoxazole.	1988	3260240
human interleukin-2 anti-idiotypes.	we describe the generation of a monoclonal anti-idiotypic (anti-id) antibody directed against affinity purified rabbit antibodies (id) to recombinant human il-2. this monoclonal antibody, 6a12, has the ability to inhibit the neutralization of il-2 activity by the id, suggesting that it may bind at or near the il-2 neutralizing site on the id. 6a12 exhibits il-2 agonist activity on pha-activated human t cells. the purified igg of 6a12 is also shown to bind to a purified soluble recombinant p55 su ...	1988	3264260
synthetic peptides of shiga toxin b subunit induce antibodies which neutralize its biological activity.	shiga toxin b chain, the binding subunit of shiga toxin, was recently purified; and the amino acid sequence of this 7,716-dalton polypeptide was determined (n.g. seidah, a. donohue-rolfe, c. lazure, f. auclair, g. t. keusch, and m. chretien, j. biol. chem. 261:13928-13931, 1986). in the present study, synthetic peptides corresponding to three overlapping sequences from the n-terminal region of this subunit were prepared. the peptides synthesized consisted of residues 5 to 18, 13 to 26, and 7 to ...	1988	3286503
synthetic peptides of shiga toxin b subunit induce antibodies which neutralize its biological activity.	shiga toxin b chain, the binding subunit of shiga toxin, was recently purified; and the amino acid sequence of this 7,716-dalton polypeptide was determined (n.g. seidah, a. donohue-rolfe, c. lazure, f. auclair, g. t. keusch, and m. chretien, j. biol. chem. 261:13928-13931, 1986). in the present study, synthetic peptides corresponding to three overlapping sequences from the n-terminal region of this subunit were prepared. the peptides synthesized consisted of residues 5 to 18, 13 to 26, and 7 to ...	1988	3286503
kingella kingae infection in healthy children.	kingella kingae is a gram-negative occasional, but normal, inhabitant of the nasopharynx. we present two new cases of this infection that occurred in previously healthy children, and compare and contrast them to other cases reported in the literature. k. kingae osteomyelitis generally has an insidious, subacute onset, whereas septic arthritis has an acute presentation. to date, all strains of k. kingae have been sensitive to penicillin, and no residual damage has been reported following osteomye ...	1988	3292580
[studies of the antigenic structure of trichophyton equinum].	in the studies on antigenic structure of t. equinum double diffusion precipitation test in agar gel and immunoelectrophoresis were used. while preparing precipitation sera in rabbits it was manifested that the best results are achieved by immunization with live antigen. in the group of metabolic antigens and water extracts of the strains of t. equinum which were tested, 10-11 serologically+ active fractions were found whereas in protein component only l1 fraction. in comparative studies based on ...	1989	2518619
[staphylococcus haemolyticus. bacterial resistance to teicoplanin and associated morpho-structural aspects].	to improve our understanding of the bases of teicoplanin resistance we studied a "one-step" mutant of s. haemolyticus from a morphological point of view. the results show that the organization of peptidoglycan is altered in the resistant s. haemolyticus strain. it is suggested that the alterations, including the spongier cell wall structure, may prevent the teicoplanin from reaching its target.	1989	2533873
susceptibility of gram positive cocci to teicoplanin. three year survey (1984-1986) at perugia university.	from 1984 to 1986, 1038 gram positive cocci (865 staphylococci and 173 enterococci) have been tested for sensitivity to teicoplanin by the agar diffusion method: only one strain (staphylococcus haemolyticus) resulted to have no zone of inhibition around the teicoplanin disk. all staphylococcus haemolyticus strains showed higher mic: 2.79 mcg/ml after 24h of incubation and 20.50 mcg/ml after 48h. the reduced teicoplanin sensitivity of staphylococcus haemolyticus was not detected by the agar diffu ...	1989	2535245
activities of daptomycin and teicoplanin against staphylococcus haemolyticus and staphylococcus epidermidis, including evaluation of susceptibility testing recommendations.	the in vitro activities of daptomycin, teicoplanin, and three other antimicrobial agents were determined against 105 strains of staphylococcus haemolyticus and 92 strains of staphylococcus epidermidis. the mics for 90% of strains tested (mic90s) of fusidic acid and rifampin were less than or equal to 0.25 microgram/ml. the mic90s of daptomycin and vancomycin were less than or equal to 4 micrograms/ml. teicoplanin had a comparable mic90 of less than or equal to 4 micrograms/ml for isolates of s. ...	1989	2543286
a b-type pdgf receptor lacking most of the intracellular domain escapes degradation after ligand binding.	the characteristics of the human b-type platelet-derived-growth-factor (pdgf) receptor expressed in chinese hamster ovary (cho) cells, were compared with those of a mutant receptor lacking all but 19 amino acids of the intracellular domain. the transfected wild-type receptor was synthesized as a 160-kda precursor that was processed to 190 kda. each cho cell expressed 30,000-100,000 receptors which bound pdgf-bb with a kd of about 0.5 nm. analysis of pdgf-ab binding yielded non-linear scatchard p ...	1989	2546765
efficacy of rifabutin in the treatment of aids-related complex.	we performed a phase 1-2 antiviral dose escalation trial of rifabutin, a rifamycin antibiotic with anti-hiv-1 activity in vitro. we followed 16 men with aids-related complex (arc) for a mean duration of 29 weeks; the maximum toxicity-limited dose of rifabutin was 2400 mg/day, which was achieved in two patients. there was some evidence of anti-hiv-1 activity in two patients, one of whom had an improvement in immune status, but 11 of the 16 patients showed a deterioration in either virologic or im ...	1989	2576627
high-performance liquid chromatographic determination of pirprofen and five of its metabolites in human plasma without hydrolysis and in human urine before and after chemical hydrolysis.	selective high-performance liquid chromatographic methods for the simultaneous determination of pirprofen and five of its metabolites either in plasma or in urine before and after chemical hydrolysis were developed. after addition of an internal standard and a buffer, the compounds were extracted from plasma using reversed-phase c18 bond-elut columns and from urine using pre-packed silica extrelut 1 columns, back-extraction into sodium hydroxide and acidification of the alkaline phase before inj ...	1989	2613803
bacterial contamination of intraocular lenses: the source of the bacteria.	in order to investigate potential sources for the bacterial contamination of intraocular lenses, specimens were taken for culture from a variety of sites. a swab from the lid margin, the operating room air, an intraocular lens which was allowed to rest on the eye, and the sodium hyaluronate were cultured during routine intercapsular lens implant surgery on 31 patients. positive cultures were obtained from six intraocular lenses. subtyping by three independent methods showed that isolates from fi ...	1989	2630346
characteristic of marrow culture and clinical relevance in leukemia with 8/21 translocation.	karyotypes and in vitro cultures of bone marrow cells from eleven patients with acute myelogenous leukemia m2 type were performed. 8/21 translocation was found in 5 patients. in all of them the cultures in vitro shared one characteristic, i.e., clusters were small and less in number. these patients could relatively easily obtain complete remission. the similarity of cfu-gm in patients with t (8; 21) may be another important biological phenotype.	1989	2630651
characteristic of marrow culture and clinical relevance in leukemia with 8/21 translocation.	karyotypes and in vitro cultures of bone marrow cells from eleven patients with acute myelogenous leukemia m2 type were performed. 8/21 translocation was found in 5 patients. in all of them the cultures in vitro shared one characteristic, i.e., clusters were small and less in number. these patients could relatively easily obtain complete remission. the similarity of cfu-gm in patients with t (8; 21) may be another important biological phenotype.	1989	2630651
solute and water transport across the pulmonary epithelium: a new chapter in lung physiology inaugurated by alfred jost.	early experiments by jost and policard demonstrated the presence of secretory activity in the lungs of the fetal rabbit. subsequent work has demonstrated a system of active ion transport across the pulmonary epithelium of the sheep fetus, in which uphill movement of chloride ions to the pulmonary lumen provides the main force for lung liquid secretion. in the last 10-20% of gestation a reabsorptive mechanism in response to beta-adrenergic stimulation is developed which is brought into play in th ...	1989	2663091
assay for sialidase using erythrocytes and peroxidase-labeled peanut lectin.	a rapid and sensitive sialidase assay method based on peroxidase-labeled peanut lectin (pna) binding to desialylated erythrocyte is described. formalinized sheep erythrocytes were used both as a stable substrate for sialidase and as a target for the lectin. in the case of sialidases from vibrio cholerae and arthrobacter ureafaciens, a linear relationship was observed between the amount of peroxidase-labeled pna bound to erythrocytes and the enzyme amount. binding of the lectin to sialidase-treat ...	1989	2688936
characterization of toxigenic vibrios isolated from the freshwater environment of hiroshima, japan.	the occurrence and characterization of toxigenic vibrios in surface water and sediment samples of the fresh water environment of the ohta river were studied. the membrane filter, pad preenrichment technique, followed by the placement of membranes onto thiosulfate citrate-bile salt-sucrose agar, was used for the enumeration of total vibrios. qualitative examination of pathogenic vibrios was also attempted. in addition, a survey was conducted to determine the incidence of clostridium botulinum in ...	1989	2690736
characterization of toxigenic vibrios isolated from the freshwater environment of hiroshima, japan.	the occurrence and characterization of toxigenic vibrios in surface water and sediment samples of the fresh water environment of the ohta river were studied. the membrane filter, pad preenrichment technique, followed by the placement of membranes onto thiosulfate citrate-bile salt-sucrose agar, was used for the enumeration of total vibrios. qualitative examination of pathogenic vibrios was also attempted. in addition, a survey was conducted to determine the incidence of clostridium botulinum in ...	1989	2690736
pemphigoid, pemphigus and desmoplakin as antigenic markers of differentiation in normal and tumorigenic mouse keratinocyte lines.	the expression of differentiation stages in a murine epidermal cell transformation model has been investigated as a basis for studies of chemically-induced differentiation. antibodies in sera of patients with the autoimmune diseases bullous pemphigoid and pemphigus vulgaris exhibit specific reactivity to antigenic determinants of basal and spinous cells, respectively, in sections of mouse and human epidermis. in addition, spinous cells in epidermis are reactive with a mouse monoclonal antibody t ...	1989	2691098
falciparum malaria--present day problems. an experience with 425 cases.	clinical details and present day problems encountered in 425 cases of falciparum malaria (pf) are reported. 10.11% had taken chloroquine prior to reporting to us. parasitic count done in 23.05% cases lacked correlation with severity of disease. pattern of fever varied markedly but 5.4% were afebrile throughout and presented only with bodyache and malaise. apyrexial spell was noted in 5.64%. 28.70% had typical facial looks of anaemia and sallow complexion. cerebral symptoms were noted in 3.05%. o ...	1989	2693436
precision of bioassays for apparent and true metabolizable energy adjusted to zero nitrogen balance.	formulae are presented for calculating the variance of estimates of amen and tmen obtained from several commonly used bioassay protocols. formulae are also developed for calculating the variance of the difference between bioavailable energy (be) estimates of two materials tested in the same bioassay. methods are described for determining the allocation of experimental units among dietary treatments that minimizes the variance of amen or tmen estimates in bioassays based upon a completely randomi ...	1989	2704668
comparative virulence of human isolates of coagulase-negative staphylococci tested in an infant mouse weight retardation model.	human infections caused by coagulase-negative staphylococci have steadily increased in numbers and severity. causes may be the use of artificial prostheses, immunocompromising chemotherapy and radiation therapy, and sophisticated surgical techniques, to name a few. although the infectivity of coagulase-negative staphylococci as a group has been well documented for humans, attempts to study the pathogenesis of infections caused by individual species of coagulase-negative staphylococci have been h ...	1989	2715323
antimicrobial resistance in nosocomial isolates of staphylococcus haemolyticus.	staphylococcus haemolyticus is frequently cultured from hospitalized patients and is characterized by resistance to multiple antimicrobial agents. we found that s. haemolyticus represented 70 of 524 (13%) coagulase-negative staphylococcal isolates identified by the clinical microbiology laboratories of two hospitals over 2 months. s. haemolyticus isolates were recovered from wounds (44%), urine (26%), blood (10%), and other sources (20%). all s. haemolyticus isolates were tested for susceptibili ...	1989	2729941
adverse effects of the use of unusual phenethylamine compounds sold as illicit amphetamine.	a 40-year-old man, who had regularly taken illicit amphetamine by intra-nasal inhalation for several years without ill effects, was admitted to hospital with signs of massive adrenergic overstimulation shortly after inhaling material which he had purchased in the belief that it was amphetamine. the administration of the beta-blocker practolol produced a paradoxical increase in blood pressure. after his discharge from hospital he suffered disabling feelings of anxiety for several weeks. analysis ...	1989	2770471
[epidemiologic and clinical study of infection caused by hepatitis delta virus in the health service area of la coruna].	a prospective study on 175 hepatitis hbsag positive patients was carried out between march 1986 and june 1987 in order to study the epidemiological and clinical aspects of the hvd infection. the global prevalence of this infection was 19.42%, with a statistically relevant predominance amongst intravenous drug addicts (ivda). moreover, in most cases it was associated with liver disease (91.17%). chronic liver disease had a greater incidence amongst hvd positive infected patients and presented mor ...	1989	2772347
nucleotide sequences and expression of cdna clones for boar and bull transition protein 1 and its evolutionary conservation in mammals.	during spermatogenesis, the nucleoproteins undergo several dramatic changes as the germinal cells differentiate to produce the mature sperm. with nuclear elongation and condensation, the histones are replaced by basic spermatidal transition proteins, which are themselves subsequently replaced by protamines. we have isolated cdna clones for one of the transition proteins, namely for tp1, of bull and boar. it turned out that tp1 is a small, but very basic protein with 54 amino acids (21% arginine, ...	1989	2777004
opening remarks of president fernando e. agrait on the occasion of the 50th anniversary of the cayo santiago macaque colony.		1989	2780954
sciatic nerve regeneration in the rat. validity of walking track assessment in the presence of chronic contractures.	the ability of the walking track assessment of sciatic nerve function to correlate with results of a nerve repair has been well documented following acute nerve injury and after recovery of the nerve following nerve repair. the long-term effects of partial denervation upon the lower extremity of the rat include the development of chronic contractures due to residual partial muscle paralysis and imbalance. the present study evaluated the ability of the walking track technique to portray accuratel ...	1989	2796718
[electrophysiological analysis of the interaction of the hypothalamic preoptic area and the nonspecific system of the thalamus in the rabbit].		1989	2806656
smoking and other tobacco use.		1989	2815598
smoking and other tobacco use.		1989	2815598
evaluation of spontaneous resistance to glycopeptide antibiotics in staphylococcal populations.		1989	16312453
[characteristics of the dental arches in orthognathic bite and sagittal anomalies of the bite in preschool children].		1989	267348
[endangering the professional work through eye diseases--endangering the eye through dental work].		1990	268104
proceedings: dorsal column and transcutaneous electrical stimulation for intractable pain.		1990	171921
nursing management process in the financial area.		1990	258789