Publications

effect of some nutritional and environmental parameters on the production of diacetyl and on starch consumption by pediococcus pentosaceus and lactobacillus acidophilus in submerged cultures.	three series of 5-day submerged cultures with pediococcus pentosaceus mitj-10 and lactobacillus acidophilus hansen 1748 were carried out in starch-based media, and the effect of cultural factors on the changes of starch, diacetyl and amylase activity determined. in axenic cultures, ped. pentosaceus mitj-10 produced more diacetyl (63.27 mg l(-1)) by adding glucose, yeast extract and caco3 (p < 0.01), at 28 degrees c (p < 0.05); but more starch was consumed (18.4 g l(-1)) in the absence of glucose ...	2000	10735253
enterocin 012, a bacteriocin produced by enterococcus gallinarum isolated from the intestinal tract of ostrich.	enterococcus gallinarum strain 012, isolated from the duodenum of ostrich, produced enterocin 012 which is active against ent. faecalis, lactobacillus acidophilus, lact. sake, listeria innocua, propionibacterium acidipropionici, propionibacterium sp., clostridium perfringens, pseudomonas aeruginosa and salmonella typhimurium. one of the four pathogenic strains of escherichia coli isolated from the intestinal tract of ostrich was inhibited by enterocin 012. no antimicrobial activity was recorded ...	2000	10736005
isolation and identification of tetracycline resistant lactic acid bacteria from pre-packed sliced meat products.	in recent years, the food chain has been recognised as one of the main routes for transmission of antibiotic resistant bacteria between the animal and human population. in this regard, the current study aimed to investigate if tetracycline resistant (tetr) lactic acid bacteria (lab) are present in ready-to-eat modified atmosphere packed (map) sliced meat products including fermented dry sausage, cooked chicken breast meat and cooked ham. from population graphs based on doubling tetracycline conc ...	2000	10930081
analysis of the genetic determinant for production of the pediocin p of pediococcus pentosaceus pep1.	pediococcus pentosaceus pep1 is a vacuum-packaged turkish sausage isolate which produces a potentially novel bacteriocin of the pediocin (anti-listeria) family of peptides designated as pediocin p. curing experiments and plasmid profile analysis indicated that both bacteriocin immunity and production determinants were linked and encoded by 9.0 mda plasmid, phd1.0. attempts to transform purified plasmid phd1.0 into recipient escherichia coli jm109 cells by electroporation were successful but none ...	2000	10986669
inducible metabolism of phenolic acids in pediococcus pentosaceus is encoded by an autoregulated operon which involves a new class of negative transcriptional regulator.	pediococcus pentosaceus displays a substrate-inducible phenolic acid decarboxylase (pad) activity on p-coumaric acid. based on dna sequence homologies between the three pads previously cloned, a dna probe of the lactobacillus plantarum pdc gene was used to screen a p. pentosaceus genomic library in order to clone the corresponding gene of this bacteria. one clone detected with this probe displayed a low pad activity. subcloning of this plasmid insertion allowed us to determine the part of the in ...	2000	11073918
expression in escherichia coli of native and chimeric phenolic acid decarboxylases with modified enzymatic activities and method for screening recombinant e. coli strains expressing these enzymes.	four bacterial phenolic acid decarboxylases (pad) from lactobacillus plantarum, pediococcus pentosaceus, bacillus subtilis, and bacillus pumilus were expressed in escherichia coli, and their activities on p-coumaric, ferulic, and caffeic acids were compared. although these four enzymes displayed 61% amino acid sequence identity, they exhibit different activities for ferulic and caffeic acid metabolism. to elucidate the domain(s) that determines these differences, chimeric pad proteins were const ...	2001	11229892
[molecular structure of the locus for sucrose utilization by lactobacillus plantarum: comparison with pediococcus pentosaceus].	structure of the sucrose utilization locus in a lactobacillus plantarum type strain was studied using pcr and southern hybridization. restriction map analysis revealed its high similarity to the sequenced sucrose utilization locus of pediococcus pentosaceus psrq1. the l. plantarum locus proved containing oppositely oriented scra and the scrbragl operon, but not agas. the l. plantarum sucrase gene (scrb) was partly sequenced. a higher (98.6%) homology was revealed between scrb than between the 16 ...	2001	11234378
dry sausage fermented by lactobacillus rhamnosus strains.	the ability of three probiotic lactobacillus rhamnosus strains gg, e-97800 and lc-705 and one commercial pediococcus pentosaceus starter strain (control) to produce dry sausage was studied. during the fermentation process the numbers of inoculated lactic acid bacteria increased from approx. 7 log10 to 8-9 log10 cfu/g and the ph values decreased from 5.6 to 4.9-5.0. the sensory test indicated that the dry sausages fermented by l. rhamnosus lc-705 were inferior to the control sausages. the presenc ...	2001	11252505
phenotypic and genotypic characterization of pediococcus strains isolated from human clinical sources.	seventy-two strains of pediococci isolated from human clinical sources were characterized by conventional physiological tests, chromogenic enzymatic tests, analysis of whole-cell protein profiles (wcpp) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and analysis of chromosomal dna restriction profiles by pulsed-field gel electrophoresis (pfge). conventional tests allowed identification of 67 isolates: 52 strains were identified as pediococcus acidilactici, 15 strains were identifi ...	2001	11283035
detection of lactobacillus, pediococcus, leuconostoc, and weissella species in human feces by using group-specific pcr primers and denaturing gradient gel electrophoresis.	denaturing gradient gel electrophoresis (dgge) of dna fragments generated by pcr with 16s ribosomal dna-targeted group-specific primers was used to detect lactic acid bacteria (lab) of the genera lactobacillus, pediococcus, leuconostoc, and weissella in human feces. analysis of fecal samples of four subjects revealed individual profiles of dna fragments originating not only from species that have been described as intestinal inhabitants but also from characteristically food-associated bacteria s ...	2001	11375166
optimum production, stability, partial purification and inhibitory spectrum of antimicrobial compounds produced by pediococcus pentosaceus di.	the optimum conditions for production of antimicrobial compounds by pediococcus pentosaceus di were studied. the antimicrobial compounds in cell free fermented medium were extracted (methanol-acetone), then tested for inhibitory activity against fourteen bacterial strains and its stability to heating, various ph and proteolytic enzymes. in addition, methanol-acetone extract was partial purified through sephadex g-100 column and thin-layer chromatography. production of antimicrobial compounds wer ...	2001	11379284
the effect of lactobacillus buchneri and other additives on the fermentation and aerobic stability of barley silage.	whole-plant barley (39.4% dry matter) was treated with various chemical and biological additives to assess their effects on silage fermentation and aerobic stability. treatments were untreated forage, forage treated with several amounts of lactobacillus buchneri and enzymes (l. buchneri at 1 x 10(5), 5 x 10(5), and 1 x 10(6) cfu/g of fresh forage), forage treated with an inoculant containing (lactobacillus plantarum, pediococcus pentosaceus, propionibacterium freudenreichii, and enzymes), or for ...	2001	11384041
[biological deacidification of wines using lactic-acid bacteria and yeasts].	based on a study of 200 lactic-acid bacteria monocultures and 30 associating lactic bacteria and yeasts cultures, a stable association was created formed by leuconostoc oenos, pediococcus pentosaceus and saccharomyces cerevisiae yeasts, intended for the biological deacidification of wine. physiology of microorganisms and their effect on the wine chemical composition was studied. by means of selective association, high quality fine wines were produced from the high-acid wines.	2001	11530675
the gram-positive tonsillar and nasal flora of piglets before and after weaning.	to investigate gram-positive nasal and tonsillar microbial flora of piglets before and after weaning.	2001	11851806
chronic progressive myelopathy: investigation with csf electrophoresis, evoked potentials, and ct scan.	chronic progressive myelopathy (cpm) is a difficult clinical problem. many patients who present with cpm turn out to have a spinal form of multiple sclerosis (ms), but until there is clear lesion dissemination, a definite clinical diagnosis cannot be made. we have looked for ms-related abnormalities in 72 patients with cpm. the mean age of onset was 42 years, mean duration was ten years, and mean kurtzke disability rating was 4.5. studies performed were cerebrospinal fluid electrophoresis for ol ...	2001	92910
[comparative study of the average longevity of males and females in non-soviet countries].		2001	90391
[clinical aspects, diagnosis and treatment of cancer of the large intestine].		2001	93232
incidence of myeloid leukaemia in lancashire.		2001	92695
pacing-induced alternate wenckebach periods: incidence and clinical significance.	alternate wenckebach periods have been defined as episodes of 2:1 atrioventricular (av) block in which conducted p waves exhibit progressive pr prolongation until two or three successively blocked p waves. ocurrence of this phenomenon during atrial pacing has been established. thirty-six patients were studied and right atrial pacing was achieved at increasing rates up to 350 beats/min in order to induce alternate wenckebach periods. his bundle recordings were obtained in every patient. the patie ...	2001	95224
flavour profiles of dry sausages fermented by selected novel meat starter cultures.	probiotic or bioprotective lactobacillus rhamnosus strains gg, lc-705 and e-97800 as well as pediococcus pentosaceus e-90390 and lactobacillus plantarum e-98098 were studied for their ability to act as main fermenting organisms in the manufacturing process of dry sausages. in the preliminary tests, their abilities to produce lactic acid and biogenic amines, histamine or tyramine, were studied in mrs broth and analysed by high-performance liquid chromatography. the strains produced higher or equa ...	2001	22062105
effect of starter culture and storage temperature on the content of biogenic amines in dry fermented sausage polican.	water activity, ph, microbial counts (total counts/tcm/, coliforms, enterobacter, proteus, pseudomonas, escherichia and lactobacillus spp., respectively), and seven biogenic amines (ba) were determined in dry fermented sausage 'polican' produced using starter culture a (lactobacillus sakei, staphylococcus carnosus, s. xylosus) or b (l. sakei, s. carnosus, pediococcus pentosaceus), ripened 42 days, and subsequently stored at 8 or 22°c 60 days. counts of lactobacilli were higher and tcm lower in t ...	2001	22062781
seat-belts.		2001	87918
[j. a. comenius' magia et scientia].		2002	135380
[menetrier's disease: protein-losing gastropathy and antiplasmin therapy (author's transl)].		2002	135109
the diagnosis of genitourinary neoplasms.		2002	123911
[histochemical changes in the morphologic structure of tissues in women with inflammatory diseases of the internal genital organs].		2002	124045
fermentation and microflora of plaa-som, a thai fermented fish product prepared with different salt concentrations.	plaa-som is a thai fermented fish product prepared from snakehead fish, salt, palm syrup and sometimes roasted rice. we studied the effects of different salt concentrations on decrease in ph and on microflora composition during fermentation. two low-salt batches were prepared, containing 6% and 7% salt (w/w) as well as two high-salt batches, containing 9% and 11% salt. ph decreased rapidly from 6 to 4.5 in low-salt batches, whereas in high-salt batches, a slow or no decrease in ph was found. lac ...	2002	11883675
growth and aroma production by staphylococcus xylosus, s. carnosus and s. equorum--a comparative study in model systems.	a laboratory medium inoculated with 20 different staphylococcus strains was prepared in accordance with a full factorial experimental design investigating the effect of temperature, ph, nacl and glucose on growth. the 12 strains most suited to growth in a fermented meat environment were inoculated in sausage minces together with pediococcus pentosaceus, incubated at 25 degrees c for 1 week and the produced aroma compounds collected. the data were analysed by multiple linear regression and partia ...	2002	11999121
persistence of escherichia coli o157:h7 in barley silage: effect of a bacterial inoculant.	the effect of a lactic acid producing bacterial (lab) inoculant on the elimination of escherichia coli o157:h7 from barley forage was assessed.	2002	12147077
a pcr-based method for identification of lactobacilli at the genus level.	we developed a polymerase chain reaction (pcr)-based method for the identification of lactobacilli at the genus level. one specific primer, lblma1-rev, was designed by analysing similarities between the nucleotide sequence of the spacer between the 16s and 23s rrna genes in a number of lactobacillus strains. amplification with lblma1-rev and r16-1, a universal primer, generated a pcr product for 23 lactobacillus species. electrophoresis did not reveal any discrete bands when escherichia coli, la ...	2002	12351242
the effect of temperature on the growth and persistence of salmonella in fermented liquid pig feed.	two studies were conducted to investigate the effect of temperature on the fate of salmonella enterica subsp. enterica serovar typhimurium dt104:30 in fermented liquid pig feed. these were (1) by co-inoculation of feed with s. typhimurium dt104:30 and pediococcus pentosaceus, as the fermenting organism, and (2) by fermenting feed for 48, 72 or 96 h prior to inoculation with s. typhimurium dt104:30. in co-inoculated feed incubated at 20 degrees c, s. typhimurium dt104:30 persisted for at least 72 ...	2002	12382689
microbiological and fermentation characteristics of togwa, a tanzanian fermented food.	selected microbiological and metabolic characteristics of sorghum, maize, millet and maize-sorghum togwa were investigated during natural fermentation for 24 h. the process was predominated by lactic acid bacteria (lab) and yeasts. the mesophiles, lactic acid bacteria, and yeasts increased and the enterobacteriaceae decreased to undetectable levels within 24 h. the isolated microorganisms were tentatively identified as lactobacillus plantarum, lactobacillus brevis, lactobacillus fermentum, lacto ...	2003	12423921
purification and genetic characterization of plantaricin nc8, a novel coculture-inducible two-peptide bacteriocin from lactobacillus plantarum nc8.	a new, coculture-inducible two-peptide bacteriocin named plantaricin nc8 (plnc8) was isolated from lactobacillus plantarum nc8 cultures which had been induced with lactococcus lactis mg1363 or pediococcus pentosaceus fbb63. this bacteriocin consists of two distinct peptides, named alpha and beta, which were separated by c(2)-c(18) reverse-phase chromatography and whose complementary action is necessary for full plantaricin nc8 activity. n-terminal sequencing of both purified peptides showed 28 a ...	2003	12514019
phylogenetic diversity of lactic acid bacteria associated with paddy rice silage as determined by 16s ribosomal dna analysis.	a total of 161 low-g+c-content gram-positive bacteria isolated from whole-crop paddy rice silage were classified and subjected to phenotypic and genetic analyses. based on morphological and biochemical characters, these presumptive lactic acid bacterium (lab) isolates were divided into 10 groups that included members of the genera enterococcus, lactobacillus, lactococcus, leuconostoc, pediococcus, and weissella: analysis of the 16s ribosomal dna (rdna) was used to confirm the presence of the pre ...	2003	12514026
bacteriocins from pediococcus pentosaceus l and s from pork meat.	two strains of pediococcus pentosaceus were isolated from refrigerated pork and found to produce antimicrobial substances that may inhibit foodborne pathogens and have potential as natural food preservatives. they were named p. pentosaceus l and s. the antimicrobial substances were purified to electrophoretical homogeneity by chloroform extraction and designated pentocins l and s with molecular masses (m) of 27 and 25 kda, respectively. both pentocins also had broad inhibition spectra and were t ...	2003	12568574
broad and complex antifungal activity among environmental isolates of lactic acid bacteria.	more than 1200 isolates of lactic acid bacteria isolated from different environments were screened for antifungal activity in a dual-culture agar plate assay. approximately 10% of the isolates showed inhibitory activity and 4% showed strong activity against the indicator mould aspergillus fumigatus. the antifungal spectra for 37 isolates with strong activity and five isolates with low or no activity were determined. several of the strains showed strong inhibitory activity against the moulds a. f ...	2003	12594034
autolytic activity and pediocin-induced lysis in pediococcus acidilactici and pediococcus pentosaceus strains.	to evaluate the autolytic phenotype of pediococcus acidilactici and p. pentosaceus, the peptidoglycan hydrolases content and the effect of pediocin ach/pa-1 and autolysins on cell lysis.	2003	12631191
use of starter cultures of lactic acid bacteria and yeasts in the preparation of togwa, a tanzanian fermented food.	starter cultures of lactic acid bacteria (lactobacillus brevis, lactobacillus cellobiosus, lactobacillus fermentum, lactobacillus plantarum and pediococcus pentosaceus) and yeasts (candida pelliculosa, candida tropicalis, issatchenkia orientalis and saccharomyes cerevisiae) isolated from native togwa were tested singly or in combination for their ability to ferment maize-sorghum gruel to produce togwa. all species of bacteria showed an ability to ferment the gruel as judged by lowering the ph fr ...	2003	12745235
proteolytic activities in togwa, a tanzanian fermented food.	proteolytic activities were investigated in sorghum-based togwa prepared by natural fermentation and using starter cultures previously isolated from the native product, i.e., lactobacillus brevis, lactobacillus cellobiosus, lactobacillus fermentum, lactobacillus plantarum, pediococcus pentosaceus, and issatchenkia orientalis in coculture with either l. brevis or l. plantarum. both proteinase and aminopeptidase activities were substantially higher in naturally fermented togwa than in those with s ...	2003	12781948
potential osmoprotectants for the lactic acid bacteria pediococcus pentosaceus and tetragenococcus halophila.	the physiological responses of the lactic acid bacteria pediococcus pentosaceus and tetragenococcus halophila (formely known as p. halophila), subjected to osmotic stress in the presence of molecules known to act as osmoprotectants for other bacteria were studied. in a defined medium, glycine betaine, dimethylsulfonioacetate, choline, proline and l-carnitine were able to relieve inhibition of growth at 0.8 m nacl. the five compounds were shown to efficiently compete with glycine betaine transpor ...	2003	12781949
the effects of using lactic acid bacteria inoculants in maize silage on the formation of biogenic amines.	silages from five ripened varieties of silage maize with dry matter contents ranging between 275 and 410 g/kg were prepared in five laboratory experiments. whole-plant maize was fermented at 22 degrees c and silages were then stored at the same temperature for 4 months. spontaneously fermented silages were prepared as control variants and compared with silages inoculated with commercial strains of lactobacillus plantarum, lactobzcillus buchneri and a mixed preparation microsil containing l. plan ...	2003	14620909
two distinct pathways for the formation of hydroxy fa from linoleic acid by lactic acid bacteria.	twenty-three of 86 strains of lactic acid bacteria transformed linoleic acid into hydroxy fa. two distinct conversion pathways were in operation. two strains of lactobacillus acidophilus and a strain of pediococcus pentosaceus produced 13(s)-hydroxy-9-octadecenoic acid 113(s)-oh 18:11 and 10,13dihydroxyoctadecanoic acid (10,13-oh 18:0) as main and minor products, respectively, whereas 13 strains, including l. casei subsp. casei, l. paracasei subsp. paracasei, l. rhamnosus, l. lactis subsp. cremo ...	2003	14870930
a case of gastric leiomyoblastoma with metastasis to the liver.		2003	262300
[cyclic amp. biochemical and clinical aspects].		2003	166470
[state of dental health of a group of handicapped adult in jönköping].		2003	150056
hodgkin's disease in children.		2003	149946
synthetic arterial grafts. iii. the outcome in 22 patients with synthetic axillofemoral bypass grafts.	during the 4-year period 1973--76, 22 patients were operated upon with axillofemoral bypass grafts at the department of surgery in lund. they were high-risk patients with severe ischaemia of the leg or previous graft infections. fourteen of the 22 grafts were well-functioning after the longest observation time of 32 months. the operative procedure is described and postoperative compliations are reviewed. the axillofemoral bypass reconstruction is recommended as an alternative for this category o ...	2003	152457
assessing the physically disabled: social factors in rehousing.		2003	152442
the equilibrium constant and the reversibility of the reaction catalysed by nicotinamide-adenine dinucleotide kinase from pigeon liver.	the reversibility of the nad+ kinase reaction was established, and the kinetic parameters of the rate equation in the reverse direction were determined. the equilibrium constant of the reaction was determined by using the purified pigeon liver enzyme and radioactively labelled nicotinamide nucleotides. the relationship between kinetic parameters of the forward and reverse reactions is in reasonable agreement with the measured equilibrium constant. as expected from the proposed mechanism of actio ...	2003	201249
[thyroid ectopy. growth retardation].		2003	262258
[relation between endocrinology and psychiatry. some recent developments].	relations between endocrinology and psychiatry are developing in two main directions: behavioural effects of hormones. after the effects of peripheral hormones, recent works are concerned with a.c.t.h. and antidiuretic hormone (which interfere with memory process) and with hypothalamic peptides (which might have psychotropic properties, independant of their endocrine regulation function). -correlations between psychiatric syndroms and endocrine disorders. in some mentally depressed patients, dis ...	2003	165466
[eosinophilic leukosis].		2003	290268
[diagnostic problems of primary amyloidosis].	surgical liver biopsy specimen of a women aged 58 with "biliary complaints" showed amyloid deposits of unknown nature. after a nearly two-years-course of the disease the patient died of cardiac and renal failure. clinical findings and laboratory tests without abnormal serum globulins suggested primary amyloidosis. morphologically amyloid was present in the heart, tongue, striated muscles and in many parenchymatous organs and endocrine glands. by electron microscopy no difference was revealed bet ...	2003	152408
unrestricted donations are capital funds, not revenue.		2003	152389
a study of repeat prescription cards in a general practice.	a study of repeat prescription cards in a general practice revealed problems in good record keeping and patient recall. a system involving a repeat register was therefore developed to create an easily accessible practice record and a recall procedure was initiated. greater control over repeat cards is now possible.examination of the age and sex of the card-holders showed a high proportion of elderly females. by scrutiny of the contents of the cards, a profile of prescribing in the practice was d ...	2004	522065
external factors influencing blood gas analysis: quality control revisited.		2004	517557
volatiles in a sausage surface model-influence of penicillium nalgiovense, pediococcus pentosaceus, ascorbate, nitrate and temperature.	thirty-two agar sausage models were arranged in a 2((5-1)) fractional factorial design to analyse the effects of penicillium nalgiovense growth, pediococcus pentosaceus starter, sodium ascorbate, sodium nitrate and temperature on 79 volatiles produced during incubation. the model focused on the outer 10 millimeters of sausages. ascorbate addition showed clear antioxidative effects, and reduced the amount of more than half of all volatiles but increased concentrations of 2-methyl-propanal and 3-m ...	2004	22064148
growth and production of volatiles by staphylococcus carnosus in dry sausages: influence of inoculation level and ripening time.	three sausage batches inoculated with normal inoculation level of pediococcus pentosaceus (5×10(6) cfu/g) and with low, intermediate, and high inoculation levels of staphylococcus carnosus (10(5), 5×10(6), 5×10(7) cfu/g, respectively) were produced. cell counts and formation of volatiles were followed throughout a ripening period of three weeks. the staphylococci exhibited the fastest growth in sausages with a low inoculation level, whereas growth was only moderate in sausages with a high initia ...	2004	22061519
a fermented meat model system for studies of microbial aroma formation.	a fermented meat model system was developed, by which microbial formation of volatiles could be examined. the model was evaluated against dry, fermented sausages with respect to microbial growth, ph and volatile profiles. fast and slowly acidified sausages and models were produced using the starter cultures pediococcus pentosaceus and staphylococcus xylosus. volatiles were collected and analysed by dynamic headspace sampling and gc-ms. the analysis was primarily focused on volatiles arising from ...	2004	22063950
addition of a-ketoglutarate enhances formation of volatiles by staphylococcus carnosus during sausage fermentation.	the effect of leucine and a-ketoglutarate addition on transamination of branched-chain amino acids was studied in model minces inoculated with pediococcus pentosaceus and staphylococcus carnosus. leucine addition changed the ratio of volatile breakdown products of leucine, isoleucine and valine but not the total amount of the volatiles and it was concluded that the amount of free amino acids does not limit transamination of amino acids. the addition of a-ketoglutarate resulted in increased level ...	2004	22061822
effect of starter culture, spice mix and storage time and temperature on biogenic amine content of dry fermented sausages.	two types of dry fermented sausage differing in spicing mixture and the diameter (low content of red pepper+diameter 80 mm, h-sausage; high content of red pepper+diameter 55 mm, p-sausage, respectively) were produced in parallel with two different starter cultures (pediococcus pentosaceus+staphylococcus carnosus, b-samples and s. carnosus+staphylococcus xylosus+lactobacillus farciminis, f-samples, respectively). the sausages were ripened 21 days and subsequently stored 91 days at the room temper ...	2004	22061810
legionnaires' disease. a cause of lung abscess.		2004	368366
aortic valve replacement in a case of ehlers danlos syndrome.	a case of ehlers danlos syndrome is described in a 54-year-old caucasian male. he had calcific aortic valve disease and dilatation of the ascending aorta. an aortic valve replacement was performed using a starr prosthesis.	2004	383121
clifford d. evans.		2004	383130
the need for health care--a pilot survey with general practice.	a feasibility study was carried out in two places in wiltshire of a method of ascertaining the morbidity of the population. traditional medical history taking and examination of the individuals were completed in a random sample of the population, of whom 89 per cent were contacted. the method appears feasible for a survey of sufficient size to measure morbidity and the study gave useful preliminary indications of unmet health care need. the cost of such a larger survey would be small in relation ...	2004	522069
lung biopsy in sarcoidosis.		2004	421597
the myelodysplasia and hydrocephalus program in nebraska: a 15 year review of costs and benefits.		2004	412114
application of laser raman and infrared spectroscopy to the analysis of membrane structure.		2004	383152
purification and characterization of bacteriocin from pediococcus pentosaceus accel.	the pediococcus pentosaceus accel bacteriocin was purified to electrophoretical homogeneity by cell adsorption-desorption and superose 12 fast performance liquid chromatography (fplc). the purified bacteriocin, with a molecular mass of 17.5 kda and an n-terminal sequence of -kyygngvtxgkhsxxvdxg-, belongs to class iia and is designated pediocin accel. it was inactivated by various proteases and stable at ph 2.0-6.0 and <100 degrees c. more than 80% activity was left even after 15 min of heating a ...	2004	14995112
relative ability of orally administered lactobacillus murinus to predominate and persist in the porcine gastrointestinal tract.	five porcine-derived lactobacillus or pediococcus isolates administered to pigs (n = 4), either singly or as a combination at approximately 10(10) cfu per day varied with respect to intestinal survival and persistence. two lactobacillus murinus strains survived best and were excreted at approximately 10(7) to 10(8) cfu/g of feces. in contrast, pediococcus pentosaceus dpc6006 had the lowest fecal count at approximately 10(5) cfu/g and was excreted at a significantly lower level than both l. murin ...	2004	15066778
cloning, deletion, and characterization of padr, the transcriptional repressor of the phenolic acid decarboxylase-encoding pada gene of lactobacillus plantarum.	lactobacillus plantarum displays a substrate-inducible pada gene encoding a phenolic acid decarboxylase enzyme (pada) that is considered a specific chemical stress response to the inducing substrate. the putative regulator of pada was located in the pada locus based on its 52% identity with padr, the pada gene transcriptional regulator of pediococcus pentosaceus (l. barthelmebs, b. lecomte, c. diviès, and j.-f. cavin, j. bacteriol. 182:6724-6731, 2000). deletion of the l. plantarum padr gene cle ...	2004	15066807
diversity of lactic acid bacteria isolated from aoc salers cheese.	the objective of this work was to describe the diversity of lactic acid bacteria in traditional raw milk salers cheeses at the species and strain levels. the characterization of 381 strains isolated during ripening and various strain collections was investigated using physiological analysis and molecular techniques: rep-pcr, species and genus specific amplifications and the sequence analysis of 16s rdna for strain typing and taxonomic identification. the strains belonged to lactobacillus plantar ...	2004	15190953
characterization of mesentericin st99, a bacteriocin produced by leuconostoc mesenteroides subsp. dextranicum st99 isolated from boza.	lactic acid bacteria isolated from boza, a cereal-fermented beverage from belogratchik, bulgaria, were screened for the production of bacteriocins. with the first screening, 13 of the 52 isolates inhibited the growth of listeria innocua and lactobacillus plantarum. the cell-free supernatant of one of these strains, classified as leuconostoc mesenteroides subsp. dextranicum st99, inhibited the growth of bacillus subtilis, enterococcus faecalis, several lactobacillus spp., lactococcus lactis subsp ...	2004	15252717
population dynamics of the constitutive biota of french dry sausages in a pilot-scale ripening chamber.	the population dynamic of constitutive biota on 84 samples belonging to two different types of french fermented dry sausages during the ripening process in a pilot-scale ripening chamber was investigated. samples were analyzed in three steps of their production: fresh product, first drying stage, and finished product. in addition, 180 strains of lactic acid bacteria were identified using a miniaturized biochemical procedure of characterization. in general, the number of lactic acid bacteria that ...	2004	15508649
the ph-unrelated influence of salt, temperature and manganese on aroma formation by staphylococcus xylosus and staphylococcus carnosus in a fermented meat model system.	the influence of manganese (0.01-0.1-1.0 microg/g), temperature (15-24 degrees c) and salt (3-4% w/w) on volatile formation in model minces inoculated with pediococcus pentosaceus and either staphylococcus xylosus or staphylococcus carnosus was studied in a full factorial experiment. in order to study the direct, ph-unrelated effect of the parameters, data were analysed by use of multiple linear regression and partial least-squares regression both before and after transformation of the volatile ...	2004	15527916
gene organization and sequences of pediocin ach/pa-1 production operons in pediococcus and lactobacillus plasmids.	to determine the locations and sequences of pediocin ach production genes in pediococcus parvulus ato77 from vegetables, lactobacillus plantarum whe92 from muenster cheese, and a lactose-fermenting isolate pediococcus pentosaceus s34 from buffalo milk.	2005	15613003
biodiversity of lactobacillus sanfranciscensis strains isolated from five sourdoughs.	five different sourdoughs were investigated for the composition of lactic acid bacteria (lab) and the biodiversity of lactobacillus sanfranciscensis strains.	2005	15836738
use of hydrolysates from atlantic cod (gadus morhua l.) viscera as a complex nitrogen source for lactic acid bacteria.	hydrolysates of cod viscera were tested as an alternative to commonly used complex nitrogen sources (peptones and/or extracts) for the type strains of the lactic acid bacteria lactococcus lactis, lactobacillus acidophilus, lactobacillus helveticus, lactobacillus casei, lactobacillus sakei and pediococcus pentosaceus. comparative studies with mrs-like media containing different nitrogen sources showed that all the fish hydrolysates performed equally well or better than commercial extracts/peptone ...	2005	15941626
development of solution phase hybridisation pcr-elisa for the detection and quantification of enterococcus faecalis and pediococcus pentosaceus in nurmi-type cultures.	nurmi-type cultures (ntcs), derived from the fermentation of caecal contents of specifically pathogen-free (spf) birds, have been used successfully to control salmonella colonisation in chicks. these cultures are undefined in nature and, consequently, it is difficult to obtain approval from regulatory agencies for their use as direct fed microbials (dfms) for poultry. progress towards the generation of effective defined probiotics requires further knowledge of the composition of these cultures. ...	2005	15949857
assessment of the effects of nurmi-type cultures and a defined probiotic preparation on a salmonella typhimurium 29e challenge in vivo.	the effects of treatment with an undefined commercial nurmi-type culture (ntc), cultured cecal contents, and a dual-strain probiotic, containing enterococcus faecalis and pediococcus pentosaceus, on salmonella typhimurium colonization were evaluated in a specific-pathogen-free bird model. two sets of trials were performed, and each study was arranged as a randomized complete block design with three treatments. treatments consisted of (i) control, (ii) commercial ntc, and (iii) cultured cecal con ...	2005	15954712
crystallization and preliminary x-ray crystallographic analysis of the pediocin immunity protein (pedb) from pediococcus pentosaceus at 1.35 a resolution.	pedb, a bacterial immunity protein conferring immunity to a newly identified pediocin (pediocin pp-1), was crystallized by the hanging-drop vapor diffusion method at 296 k. a 1.35 a data set has been collected from a single crystal at 100 k using synchrotron-radiation source. the pedb crystals belong to the hexagonal space group p6(2) or p6(4), with unit cell parameters a = b = 62.2, c = 39.9 a. analysis of the packing density shows that the asymmetric unit probably contains one molecule with a ...	2005	16019271
nucleotide sequence, structural organization and host range of prs4, a small cryptic pediococcus pentosaceus plasmid that contains two cassettes commonly found in other lactic acid bacteria.	the complete nucleotide sequence of the cryptic plasmid prs4 (3550 bp) from pediococcus pentosaceus rs4 was determined. sequence analysis revealed the presence of three open reading frames (orfs). the putative protein coded by orf 1 showed 93% identity with the mobilization protein of lactobacillus casei plasmid plc88 and 94% identity with a sequenced fragment of the mobilization protein of p. damnosus plasmid pf8801, suggesting a common origin for these three mobilization proteins. the putative ...	2005	16054305
identification of predominant lactic acid bacteria isolated from traditionally fermented vegetable products of the eastern himalayas.	gundruk, sinki and khalpi are lactic-fermented vegetable products of sikkim in india, and inziangsang is a fermented leafy vegetable product of nagaland and manipur in india. a total of 65 samples of gundruk (25), sinki (12), khalpi (25) and inziangsang (3) were analysed for microbial counts. the population of lactic acid bacteria (lab) as well as aerobic mesophilic counts were at the level of 10(7) cfu g(-1). yeasts were detected only in few samples of sinki and khalpi. no moulds were detected. ...	2005	16055218
effect of culture conditions on production of butter flavor compounds by pediococcus pentosaceus and lactobacillus acidophilus in semisolid maize-based cultures.	two series of 120 h mixed cultures of pediococcus pentosaceus mitj-10 and lactobacillus acidophilus hansen 1748 were grown in semisolid maize-based media, applying an orthogonal factorial design in order to establish the most adequate parameters to get compounds related to butter-like flavor. conditions of maize pre-treatment established in the first series were hot lime-treatment for 20 min, grinding and sieving to 0.0165 in., partial defatting and cooking for 25 min. while the culture conditio ...	2005	16055221
extremely low electrical current generated by porcine small intestine smooth muscle alters bacterial autolysin production.	the effect of extremely low electrical currents, identical to those generated by the gut smooth muscle, on bacterial autolysin production in vitro was tested in the present study. when stimulated with these electrical currents, the bacteria pediococcus pentosaceus 16:1 produced groups of peptidoglycan hydrolases that differed from those produced by the unstimulated (control) bacteria. the autolysins synthesized by the p. pentosaceus 16:1 under extremely low electrical currents were effective aga ...	2005	16118236
microbiological study of lactic acid fermentation of caper berries by molecular and culture-dependent methods.	fermentation of capers (the fruits of capparis sp.) was studied by molecular and culture-independent methods. a lactic acid fermentation occurred following immersion of caper berries in water, resulting in fast acidification and development of the organoleptic properties typical of this fermented food. a collection of 133 isolates obtained at different times of fermentation was reduced to 75 after randomly amplified polymorphic dna (rapd)-pcr analysis. isolates were identified by pcr or 16s rrna ...	2005	16332762
epidemiologic observations on suicides in virginia.		2005	1266370
linkage studies between hl-a and gpt polymorphisms and other genetic markers.	the phenotypes of 56 families with 126 children from the hamburg area as well as gene frequencies and segregation of the genetic markers gpt, ap, ada, ak, pgm1, pgm3, 6-pgd, che, c3, gc, tf, hp and cp were studied. in regard to linkage, the informative families were correlated to the results of hl-a and gpt typing. the linkage was tested according to the sequential test by morton (1955). see article. for other gene loci, linkage to the hl-a or gpt system could not be proved. but the positive lod ...	2005	1251571
effects of subzero temperatures on the microcirculation in the oral mucous membrane.		2005	1214600
thoughts about medical writing. xxix. authorship-who, and where?		2005	1169027
transplantation behavior of a.th and a.tl t-cell lymphomas in congenic resistant and hybrid strains.	seven spontaneously arising t-cell lymphomas originating in a.th or a.tl mice, which are congenic for the immune response gene (i) chromosomal segment were described. when transplanted into partner strains which were incompatible at the i region, the tumors were rapidly rejected. rejection was proposed to be due to the presence of antigens controlled by i-region genes.	2005	1079852
a 2-year follow-up of 72 hyperactive boys. classroom behavior and peer acceptance.	a 2-year, prospective follow-up of 72 hyperactive boys (94 per cent of the initial sample) examined classroom and home behavior, academic achievement, peer status, and depressive symptomatology for patients and a matched control group. sixty-five per cent of the sample was still on medication at follow-up. the patient group continued to manifest behavioral and academic difficulty. off-drug classroom behavior showed considerable stability from baseline to 2-year follow-up which did not appear to ...	2005	1249566
a volumetric assessment of pollen levels in the air of ann arbor, michigan.		2005	1196148
management of pathological fractures at the mayo clinic.		2005	1234120
[molecular bases of chronic granulomatous disease--analysis of the involvement of cytosol factors for nadph oxidase].	during the past 5 years, the discovery of cell-free superoxide generation system (bromberg y, pick e: cell immunol 88:213-221, 1984) has been revolutionized our understanding of phagocyte superoxide generation. using cell-free system, it was clarified that nadph oxidase for superoxide generation was comprised of components present in both the plasma membrane as well as in the cytosol. this oxidase could be kept inactive by keeping its components separated from each other within the cell and then ...	2005	1317471
[thrombotic occlusion and late lysis in the arteria subclavia lusoria dextra].	case history of a 45 years aged male patient with an aortic arch anomaly. thrombotic occlusion of the a. lusoria dextra with symptoms of a subclavian-steal syndrome and anoxia in the right hand. spontaneous lysis after 8 weeks is documentated by arteriography.	2005	1183296
[massive lower gastrointestinal hemorrhage of diverticular origin. diagnostic and therapeutic problems].		2005	1082809
thermal inactivation of the frozen thawed traditional meat starter culture, pediococcus pentosaceus, in a meat model system.	the equation, y((t))=y((0))e(kt), was fitted (r=0.9281, 0.9220 and 0.9117, respectively) to thermal inactivation data (55, 60 and 65°c) of the traditional meat starter culture pediococcus pentosaceus (10(7)cfu/ml) in a meat model system. the population reduction constant ('k') increased (about 2.5- and 3-fold) with an increase in the treatment temperature (from 55 to 60°c and from 60 to 65°c, respectively). the q(10) (55-65°c) for 'k' was 7.63. thermal treatments of 19.1, 9.0 and 3.1min (55, 60 ...	2006	22061889
behaviour of the constitutive biota of two types of spanish dry-sausages ripened in a pilot-scale chamber.	the behaviour of the constitutive biota in eighty four samples belonging to two different types of spanish dry-cured sausages during the ripening process in a pilot-scale chamber was investigated. samples were analyzed in three stages during production: fresh product, first drying stage and finished product. lactic acid bacteria (lab) and coagulase-negative cocci (cnc) were identified by the api system. in general, evolution of lab and cnc during the ripening process of spanish dry-cured sausage ...	2006	22062067
desferrioxamine provocative test: methodology for estimating iron and total iron binding capacity.		2006	2242435