Publications

phylogeny of wolbachia pipientis based on glta, groel and ftsz gene sequences: clustering of arthropod and nematode symbionts in the f supergroup, and evidence for further diversity in the wolbachia tree.	current phylogenies of the intracellular bacteria belonging to the genus wolbachia identify six major clades (a-f), termed 'supergroups', but the branching order of these supergroups remains unresolved. supergroups a, b and e include most of the wolbachiae found thus far in arthropods, while supergroups c and d include most of those found in filarial nematodes. members of supergroup f have been found in arthropods (i.e. termites), and have previously been detected in the nematode mansonella ozza ...	2005	16339946
[differentiation between the urban mosquito culex pipiens pipiens f. molestus and culex torrentium (diptera: culicidae) by the molecular genetic methods].	the molecular characters suitable for the differentiation between the autogenous form of culex pipiens pipiens (the urban mosquito) and the species culex torrentium, which was previously identified erroneously as an anautogenous form of c. pipiens pipiens (shaikevich, vinogradova, 2004), are given. these mosquitoes differ in the infection by the endosymbiotic bacteria wolbachia pipientis, 6 transitions among 247 nucleotide sequences of 3' part of the cytochrome oxidase subunit i (coi) gene of mi ...	2005	16396396
can cytoplasmic incompatibility inducing wolbachia promote the evolution of mate preferences?	the maternally inherited bacterium, wolbachia pipientis, manipulates host reproduction by rendering uninfected females reproductively incompatible with infected males (cytoplasmic incompatibility, ci). hosts may evolve mechanisms, such as mate preferences, to avoid fitness costs of wolbachia infection. despite the potential importance of mate choice for wolbachia population dynamics, this possibility remains largely unexplored. here we model the spread of an allele encoding female mate preferenc ...	2005	16033569
detection and identification of ehrlichia spp. in ticks collected in tunisia and morocco.	a broad-range 16s rrna gene pcr assay followed by partial sequencing of the 16s rrna gene was used for the detection of members of the family anaplasmataceae in ticks in north africa. a total of 418 questing ixodes ricinus ticks collected in tunisia and morocco, as well as 188 rhipicephalus ticks from dogs and 52 hyalomma ticks from bovines in tunisia, were included in this study. of 324 adult i. ricinus ticks, 16.3% were positive for ehrlichia spp., whereas only 3.4 and 2.8% of nymphs and larva ...	2005	15750072
effects of nifedipine and diltiazem on myocardial ischemia in patients with severe stable angina pectoris treated with nitrates and beta-blockers.	in a randomized, cross-over, double-blind study, the effects of nifedipine were compared with those of diltiazem in 20 patients with severe stable angina pectoris and multivessel coronary artery disease treated with nitrates and beta-blockers. the comparison was performed by bicycle ergometry, clinical evaluation, and ambulatory 24-h ecg monitoring for 7-8 weeks. as compared with placebo, both nifedipine and diltiazem significantly reduced the daily number of anginal attacks and nitroglycerin co ...	2005	1282586
[hepatotoxicity of cyproterone acetate].		2005	1410910
[repeated cesarean section. report of 100 cases].		2005	1175985
cancer communiqué. encore--a new approach to rehabilitation.		2005	1175846
academic freedom.		2005	1069648
secretogranin i/chromogranin b is a heparin-binding adhesive protein.	the major heparin-binding protein secreted by pc12 cells was purified from conditioned medium. amino-terminal sequencing of the purified protein identified it as secretogranin i/chromogranin b (sgi/chmb). the protein showed the same electrophoretic mobility and biochemical characteristics as previously reported for sgi/chmb and could be purified in high yield using a simple procedure. in vitro experiments demonstrated that sgi/chmb effectively promoted cell-substratum adhesion of nih 3t3 and pc1 ...	2005	1560226
[gastroenterological disorders in diabetes mellitus. iii. other disorders].		2005	545514
phobic response factors from the fear survey schedule.	a phobic response scale was factored from responses to the fear survey schedule. subjects were 137 male and 168 female college students. a six factor solution extracted 41.78% of the trace. the factors were as follows: i (hostile-dependence), ii(body assult), iii (developmental fears), iv (performance and evaluation), v (death evasion), and vi (nuisance animals). an analysis of sex differences by item and scale generally supported and extended previous research. some conceptual ties were made by ...	2005	1151900
[resection of chondrosarcoma of the sternum with restoration of the thoracic wall. report of a case].		2005	1081246
[hyperthyroidism during pregnancy].	the authors review the current knowledge of fetus and woman thyroid physiology during pregnancy. they analyze the abnormalities related to hyperthyroidism, and clinical and therapeutic aspects of thyrotoxicosis; in detail possible fetal complications. they suggest the practical management of thyrotoxicosis during pregnancy.	2005	1287750
regulation of glycoconjugate expression on cloned mammalian cells. studies on the growth dependence of membrane-associated/blood group h angigen.		2005	1253842
post-cataract cryptococcal endophthalmitis.		2005	1449785
appraisal of teachers in the faculty of veterinary science, university of liverpool.	in 1988, the faculty of veterinary science, university of liverpool, introduced a system of teacher appraisal, following the report of a faculty working party. the system is designed to provide information which will help the personal development of teaching skills, and also to provide the information required by the university for promoting a lecturer to senior lecturer. it incorporates opinions from students, collected formally by means of questionnaires, and from personal peers chosen by the ...	2005	1287953
primary dental care: an educational methodology for dental hygiene students.		2005	1069646
autosomal recessive inheritance of nager acrofacial dysostosis.	nager acrofacial dysostosis is a variant of mandibulofacial dysostosis with severe micrognathia, malar hypoplasia, and radial limb defects. most cases are sporadic, but autosomal recessive inheritance has been suggested. a family is reported in which two sibs are affected by this syndrome, presenting further evidence for autosomal recessive inheritance. the recognition of this syndrome as a distinct entity has important implications. after the birth of a child with orofacial malformations sugges ...	2005	3367347
[gastric secretion in gastroduodenal ulcer].		2005	545511
[vascular surgery in the aged].		2005	538948
the relationship of pretreatment family functioning to drinking behavior during follow-up by alcoholic patients.	the relationship of alcoholics' perceptions of the pretreatment functioning of their families to drinking outcomes during an 18-month follow-up was examined. family functioning was hypothesized to be predictive of drinking behavior, particularly in subjects with low assertion of autonomy scores. these individuals report greater dependency and attachment, and therefore might be more affected by the state of important relationships. the results indicated that with low autonomy male alcoholics, gre ...	2005	1449124
influence of alcohol intake on the course and consequences of spinal cord injury.		2006	1428390
[immenkamp inactivator plate and schulte interceptor].		2006	1426454
[application of the isler and ganz classification of pelvic ring fractures in clinical practice].	the classification of pelvic fractures proposed by isler and ganz was applied to 152 patients. on the basis of this experience, the following conclusion were drawn. 1. it is possible to classify all pelvic ring injuries by dividing them in lesions of the anterior and of the posterior ring segment. 2. the classification requires a thorough examination; often a ct scan is needed. the original classification of 9 out of 18 patients based on x-ray morphology had to be revised after ct examination. 3 ...	2006	1361709
[facing adversity with optimism. interview by kjell arne bakke].		2006	1489601
nutrition and head start.		2006	1458961
automated tumor registry for oncology. a va-dhcp mumps application.	the va automated tumor registry for oncology, version 2, is a multifaceted, completely automated user-friendly cancer database. easy to use modules include: automatic casefinding; suspense files; abstracting and printing; follow-up; annual reports; statistical reports; utility functions.	2006	1482866
an enhanced-response system for performing chemiluminescent and bioluminescent tests.		2006	1366493
community action research teams in population focused nursing a post diploma learning experience.		2006	1636365
chondromyxoid fibroma of the mandible. diagnostic image cytometry findings and review of the literature.	a case of chondromyxoid fibroma in the mandible of a 12-year-old boy is reported. a review of the literature is presented with emphasis on flow and image cytometric analysis and evaluation of the radiologic and histologic differences between chondromyxoid fibroma and chondrosarcoma.	2006	1574309
child abuse: does prosecution serve any useful purpose?		2006	1635513
[the basic groups of modern preparations. ii. loop diuretics. osmotic diuretics. aquaretics. uricosuric diuretics].		2006	1440324
rate of elimination of wolbachia pipientis by doxycycline in vitro increases following drug withdrawal.	wolbachia pipientis is an obligate intracellular bacterium within the family anaplasmataceae that infects many terrestrial arthropods and arthropod-transmitted nematodes (filariae). several filarial species are major human pathogens, and antibiotics with activity against wolbachia offer a promising new therapeutic approach, since the adult worms are relatively refractory to conventional anthelmintics but depend on wolbachia for reproduction and viability. in a natural filarial parasite of cattle ...	2006	16495252
asymmetrical interactions between wolbachia and spiroplasma endosymbionts coexisting in the same insect host.	we investigated the interactions between the endosymbionts wolbachia pipientis strain wmel and spiroplasma sp. strain nsro coinfecting the host insect drosophila melanogaster. by making use of antibiotic therapy, temperature stress, and hemolymph microinjection, we established the following strains in the same host genetic background: the sw strain, infected with both spiroplasma and wolbachia; the s strain, infected with spiroplasma only; and the w strain, infected with wolbachia only. the infe ...	2006	16820474
supporting collaboration through a nursing informatics curriculum stage ii.	collaboration is at the center of the process used to design, implement and evaluate an integrated informatics curriculum in a baccalaureate nursing program. this paper describes the second stage of a process to design the informatics nursing courses. the challenges to foster faculty collaborative relationships as well as to enhance the course content of all nursing informatics curriculum. a number of strategies were used to develop the collaborative efforts between the faculty and nursing staff ...	2006	1482910
[long-term outcome of a hospital series of patients with atrio-ventricular accessory pathway].	ninety five patients with a mean age of 39 +/- 19 years, 82 of whom were symptomatic, having an accessory atrioventricular bidirectional conduction pathway (wpw syndrome: 77; "concealed": 18) were followed up for an average of 7.3 +/- 2.6 years. the objectives were to analyse: the incidence and causes of death and the possible predictive factors of death due to the wpw syndrome--the influence of medical treatment and type of medication on survival and symptoms. of the 8 cardiac deaths, 6 seemed ...	2006	1363771
delusional reduplication following closed-head injury.	somatic delusions following brain injury are not uncommon, and have been well documented in the literature. this study documents a case of somatic delusion which was seen in a patient following a head injury secondary to a motorcycle accident. although perhaps not typical it serves to illustrate an interesting example of a somatic delusion following head trauma. on recovery from coma this patient reported the existence of a 'third arm' adjacent to the limb that had received the greatest impact i ...	2006	1393179
effects of picture referencing on pvc chair, love seat, and settee assemblies by students with mental retardation.	this study examined the effects of two indirect corrective feedback procedures on the assembly skills of five secondary students with moderate mental retardation. picture and video referencing conditions, during which the experimenters pointed to a picture or video screen following a performance error and requested the student do the step again, were more effective than assembly photographs, sequenced pictures, sequenced pictures and modeling, and video modeling conditions. picture referencing e ...	2006	1626083
[critical intra-abdominal infections: emerging concepts].		2006	1592313
package simplifies compliance with federal regulations.		2006	1636208
glomerular immune deposits in murine lupus models may contain histones.	two types of lupus mice, nzb/nzw f1 female hybrids and mice with graft-versus-host disease (gvhd), were studied. histones h3 and h2a were detected by immunofluorescence in glomeruli of 22/22 proteinuric gvhd and 8/12 proteinuric nzb/w f1 female mice; in non-proteinuric animals, 3/5 gvhd and 2/27 nzb/w f1 female were positive. using antibodies to histone peptides it was shown that mainly the n-terminal regions of histones h3 and h2a were exposed in glomerular deposits. western blot analysis revea ...	2006	1458682
cough variant asthma: usefulness of a diagnostic-therapeutic trial with prednisone.	cough variant asthma is characterized as a persistent, nonproductive cough with minimal or no wheezing and dyspnea. the diagnosis can be overlooked or misdisagnosed. we describe the severity of cough, the misery of some patients who have this syndrome and the usefulness of a diagnostic-therapeutic trial in ten patients with cough variant asthma. we evaluated ten patients whose chief complaint was persistent nonproductive cough. during the course of evaluation, all patients received a diagnostic- ...	2006	1471782
analysis of viral pathogenesis by in situ hybridization.		2006	1560322
shoulder dystocia.		2006	1560295
increased expression of beta-amyloid protein precursor and microtubule-associated protein tau during the differentiation of murine embryonal carcinoma cells.	expression of the genes encoding the beta/a4 amyloid protein precursor (app) and microtubule-associated protein tau was studied in an embryonal carcinoma cell line (p19) that differentiates in vitro into cholinergic neurons after treatment with retinoic acid. expression of app increased 34- (mrna) and 50-fold (protein) during neuronal differentiation; app-695 accounted for most of this increase. these remarkable increases in app expression coincided with a proliferation of neuronal processes and ...	2006	1560239
[digestive hemorrhage caused by aorto-duodenal fistula. report of 5 cases].	the aortoduodeonal fistula is a rare condition and has high mortality. we report five retrospective cases of digestive hemorrhage due to an aortoduodenal fistula (4 secondary and 1 primary) between 1986 and 1990. three of them died. attention is called to the importance of clinical suspicion and the main diagnostic methods in early diagnosis such as oral endoscopy. we also enhance the importance of emergency surgery for survival.	2006	1599763
considerations in modifying a semiquantitative food frequency questionnaire.	quantifying dietary intake remains a challenge. although advances have been made, we are still attempting to find a quick yet reliable intake method. utilizing brief, frequency-type questionnaires that have already been tested is an attractive option. however, many of us would also like to alter those questionnaires to make them more specific to our study population. the items covered in this article should help to illuminate some of the tissues that must be considered.	2006	1894908
considerations in modifying a semiquantitative food frequency questionnaire.	quantifying dietary intake remains a challenge. although advances have been made, we are still attempting to find a quick yet reliable intake method. utilizing brief, frequency-type questionnaires that have already been tested is an attractive option. however, many of us would also like to alter those questionnaires to make them more specific to our study population. the items covered in this article should help to illuminate some of the tissues that must be considered.	2006	1894908
role of technetium 99m-labeled monoclonal antibody in the management of melanoma patients.	a study was performed to assess the usefulness of a radiolabeled monoclonal antibody (moab; technetium 99m nr-ml-05 fab) as a detecting agent, as well as to evaluate its role in the overall decision-making process in the management of cutaneous malignant melanoma.	2006	1634923
can anopheles gambiae be infected with wolbachia pipientis? insights from an in vitro system.	wolbachia pipientis are maternally inherited endosymbionts associated with cytoplasmic incompatibility, a potential mechanism to drive transgenic traits into anopheles populations for malaria control. w. pipientis infections are common in many mosquito genera but have never been observed in any anopheles species, leading to the hypothesis that anopheles mosquitoes are incapable of harboring infection. we used an in vitro system to evaluate the ability of anopheles gambiae cells to harbor diverse ...	2006	17028229
increased male mating rate in drosophila is associated with wolbachia infection.	the maternally inherited bacterium wolbachia pipientis infects 25-75% of arthropods and manipulates host reproduction to improve its transmission. one way wolbachia achieves this is by inducing cytoplasmic incompatibility (ci), where crosses between infected males and uninfected females are inviable. infected males suffer reduced fertility through ci and reduced sperm production. however, wolbachia induce lower levels of ci in nonvirgin males. we examined the impact of wolbachia on mating behavi ...	2006	17040394
wolbachia endosymbiotic bacteria of brugia malayi mediate macrophage tolerance to tlr- and cd40-specific stimuli in a myd88/tlr2-dependent manner.	lymphatic filarial nematodes are able to down-regulate parasite-specific and nonspecific responses of lymphocytes and apc. lymphatic filariae are reliant on wolbachia endosymbiotic bacteria for development and survival. we tested the hypothesis that repeated exposure to wolbachia endosymbionts would drive macrophage tolerance in vitro and in vivo. we pre-exposed murine peritoneal-elicited macrophages to soluble extracts of brugia malayi female worms (bmfe) before restimulating with bmfe or tlr a ...	2006	16818783
wolbachia infection reduces sperm competitive ability in an insect.	the maternally inherited bacterium wolbachia pipientis imposes significant fitness costs on its hosts. one such cost is decreased sperm production resulting in reduced fertility of male drosophila simulans infected with cytoplasmic incompatibility (ci) inducing wolbachia. we tested the hypothesis that wolbachia infection affects sperm competitive ability and found that wolbachia infection is indeed associated with reduced success in sperm competition in non-virgin males. in the second male role, ...	2006	16777737
feminizing wolbachia in zyginidia pullula (insecta, hemiptera), a leafhopper with an xx/x0 sex-determination system.	zyginidia pullula is a grass-dwelling leafhopper characterized by a bisexual reproduction mode. in this species, some females collected in northern italy, when mated with males, gave origin to an exclusively female brood. here, we demonstrated that in these lineages an infection by a new strain of wolbachia pipientis-designated as wzygpul-was detected by amplifying and sequencing the wsp and 16s rrna genes. about half of the female progeny were characterized by intersexual phenotypes, i.e. showi ...	2006	16928646
multilocus sequence typing system for the endosymbiont wolbachia pipientis.	the eubacterial genus wolbachia comprises one of the most abundant groups of obligate intracellular bacteria, and it has a host range that spans the phyla arthropoda and nematoda. here we developed a multilocus sequence typing (mlst) scheme as a universal genotyping tool for wolbachia. internal fragments of five ubiquitous genes (gatb, coxa, hcpa, fbpa, and ftsz) were chosen, and primers that amplified across the major wolbachia supergroups found in arthropods, as well as other divergent lineage ...	2006	16936055
survival of wolbachia pipientis in cell-free medium.	wolbachia pipientis is an obligate intracellular bacterium found in a wide range of invertebrate taxa. while over ecological timescales wolbachia infections are maintained by strict maternal inheritance, horizontal transfer events are common over evolutionary time. to be horizontally transferred between organisms, wolbachia bacteria must pass through and survive an extracellular phase. we used baclight live-dead staining, pcr, and fluorescence in situ hybridization to assess the ability for puri ...	2006	16950898
toward a wolbachia multilocus sequence typing system: discrimination of wolbachia strains present in drosophila species.	among the diverse maternally inherited symbionts in arthropods, wolbachia are the most common and infect over 20% of all species. in a departure from traditional genotyping or phylogenetic methods relying on single wolbachia genes, the present study represents an initial multilocus sequence typing (mlst) analysis to discriminate closely related wolbachia pipientis strains, and additional data on sequence diversity in wolbachia. we report a new phylogenetic characterization of four genes (aspc, a ...	2006	17036209
diversifying selection and host adaptation in two endosymbiont genomes.	the endosymbiont wolbachia pipientis infects a broad range of arthropod and filarial nematode hosts. these diverse associations form an attractive model for understanding host:symbiont coevolution. wolbachia's ubiquity and ability to dramatically alter host reproductive biology also form the foundation of research strategies aimed at controlling insect pests and vector-borne disease. the wolbachia strains that infect nematodes are phylogenetically distinct, strictly vertically transmitted, and r ...	2007	17470297
new criteria for selecting the origin of dna replication in wolbachia and closely related bacteria.	the annotated genomes of two closely related strains of the intracellular bacterium wolbachia pipientis have been reported without the identifications of the putative origin of replication (ori). identifying the ori of these bacteria and related alpha-proteobacteria as well as their patterns of sequence evolution will aid studies of cell replication and cell density, as well as the potential genetic manipulation of these widespread intracellular bacteria.	2007	17584494
on the taxonomic status of the intracellular bacterium wolbachia pipientis: should this species name include the intracellular bacteria of filarial nematodes?		2007	17684235
disruption of the wolbachia surface protein gene wspb by a transposable element in mosquitoes of the culex pipiens complex (diptera, culicidae).	culex pipiens quinquefasciatus say and culex pipiens pipiens linnaeus are sibling species incriminated as important vectors of emerging and re-emerging infectious diseases worldwide. the two forms differ little morphologically and are differentiated mainly based upon ecological, behavioural, physiological and genetic traits. within the north american zone of sympatry, populations of cx. p. quinquefasciatus and cx. p. pipiens undergo extensive introgression and hybrid forms have been reported in ...	2007	17298560
symbiont genes in host genomes: fragments with a future?	while lateral transfer is the rule in the evolutionary history of bacterial and archaeal genes, events of transfer from prokaryotes to eukaryotes are rare. germline-transmitted animal symbionts, such as wolbachia pipientis, are well placed to participate in such transfers. in a recent issue of science, dunning hotopp et al. identified instances of transfer of wolbachia dna to host genomes. it is unknown whether these transfers represent innovation in animal evolution.	2007	18005738
f supergroup wolbachia in bush crickets: what do patterns of sequence variation reveal about this supergroup and horizontal transfer between nematodes and arthropods?	wolbachia pipientis, an intracellular, alpha-proteobacterium, is commonly found in arthropods and filarial nematodes. most infected insects are known to harbor strains of wolbachia from supergroups a or b, whereas supergroups c and d occur only in filarial nematodes. here, we present molecular evidence from two genes (ftsz and 16s rdna) that 2 orthopterans (the bush cricket species orocharis saltator and hapithus agitator; gryllidae: eneopterinae) are infected with wolbachia from the f supergrou ...	2007	16924406
population replacement strategies for controlling vector populations and the use of wolbachia pipientis for genetic drive.	in this video, jason rasgon discusses population replacement strategies to control vector-borne diseases such as malaria and dengue. "population replacement" is the replacement of wild vector populations (that are competent to transmit pathogens) with those that are not competent to transmit pathogens. there are several theoretical strategies to accomplish this. one is to exploit the maternally-inherited symbiotic bacteria wolbachia pipientis. wolbachia is a widespread reproductive parasite that ...	2007	18979023
widespread lateral gene transfer from intracellular bacteria to multicellular eukaryotes.	although common among bacteria, lateral gene transfer-the movement of genes between distantly related organisms-is thought to occur only rarely between bacteria and multicellular eukaryotes. however, the presence of endosymbionts, such as wolbachia pipientis, within some eukaryotic germlines may facilitate bacterial gene transfers to eukaryotic host genomes. we therefore examined host genomes for evidence of gene transfer events from wolbachia bacteria to their hosts. we found and confirmed tran ...	2007	17761848
anthraquinones as defensive compounds in eggs of galerucini leaf beetles: biosynthesis by the beetles?	eggs of leaf beetles of the tribe galerucini, subfamily galerucinae, contain polyketides that are unusual in insects: 1,8-dihydroxylated anthraquinones (chrysazin, chrysophanol) and anthrones (dithranol, chrysarobin) deterring predators. the host plants do not contain these compounds. in the present study, we tested the hypothesis that the beetles, but not bacterial or fungal microorganisms living as endosymbionts within the beetles, produce the anthraquinones. the tansy leaf beetle galeruca tan ...	2007	17879233
taxonomic status of the intracellular bacterium wolbachia pipientis.	wolbachia pipientis is a maternally inherited, intracellular bacterium found in more than 20 % of all insects, as well as numerous other arthropods and filarial nematodes. it has been the subject of a growing number of studies in recent decades, because of the remarkable effects it has on its arthropod hosts, its potential as a tool for biological control of arthropods of agricultural and medical importance and its use as a target for treatment of filariasis. w. pipientis was originally discover ...	2007	17329802
[evolution from retrotransposons to retroviruses: origin of the env gene].	in genome of drosophila melanogaster, various families of retrotransposons with different combination of functional domens and mechanisms of transposition are present. however only retrotransposons of gypsy family are retroviruses related to errantiviruses. other families seemingly appeared as intermediate forms of retroviruses evolution. despite the fact that the question on origin of retroviruses remains unclear, now the hypothesis of their origin from retrotransoposons can be considered the m ...	2007	18257289
contracts for rmos in obstetrics and gynaecology.		2007	1852332
bone marrow biopsy in myelodysplastic syndromes: morphological characteristics and contribution to the study of prognostic factors.	ten characteristics of bone marrow (bm) biopsies in paraffin sections, obtained at diagnosis from patients with myelodysplastic syndromes (mds) classified according to the fab criteria, were analysed to identify both the most relevant morphologic data and any possible influence on survival. agreement between two observers was obtained for 94% of the data. bm cellularity was increased in 63% of the cases and was higher in refractory anaemia with excess of blasts (raeb). raeb in transformation (ra ...	2007	2375920
the mhc-binding and gp120-binding functions of cd4 are separable.		2007	2387122
objectives to direct the training of emergency medicine residents on off-service rotation: otolaryngology.	this is the sixth article in a continuing series on objectives for emergency medicine training; otolaryngological objectives will be presented. otolaryngological skills and knowledge areas are frequently encountered in clinical practice. the core content in emergency medicine devotes an entire section to their listing. off-service rotations provide some one-half of all rotational-based experience in emergency medicine training. specific behaviorally based objectives for mastery of knowledge and ...	2007	2045654
therapy-related leukaemia in wegener's granulomatosis.		2007	1934930
skeletal applications of videofluoroscopy.		2007	1919376
school policies and programs on smoking and health--united states, 1988.		2007	2493573
double-blind study with fenticonazole or bifonazole lotions in pityriasis versicolor.	the efficacy of fenticonazole 2% lotion was studied in patients suffering from pityriasis versicolor in a double-blind comparison with bifonazole 1% lotion, both applied once daily for three weeks. forty-six patients were recruited and randomly allocated to one or other treatment group. clinical and mycological examinations, performed at baseline and at weekly intervals, have shown an excellent response to both treatments. two cases of mild transient desquamation were reported in the fenticonazo ...	2007	3182117
chemical contamination in office buildings.		2007	1951046
phosphoglucomutase-1 polymorphism among chinese in taiwan.	phosphoglucomutase-1 (pgm1) phenotyping of 1,128 chinese blood donors was performed by thin-layer isoelectric focusing on agarose. the pgm1 gene frequencies were: 1a, 0.6005; 1b, 0.1500; 2a, 0.1510; 2b, 0.0973, and rare variants, 0.0058. the rare variants found in this series were pgm1 w21, w2, w3, w6 and w9 (or w10) with pgm1 w21 being the most common variant among chinese with a phenotype frequency of 0.8%.	2007	1828778
transfection of wolbachia pipientis into drosophila embryos.	wolbachia is a genus of obligate intracellular alpha-proteobacteria represented by the type species wolbachia pipientis (dumler et al., 2001). wolbachia commonly reside within cytoplasmic vacuoles of arthropods and helminths (werren and windsor, 2000; casiraghi et al., 2004); vertebrate infections have not been identified. wolbachia are maternally transmitted from mothers to offspring though the embryonic cytoplasm. wolbachia are able to induce a diverse range of phenotypes in their invertebrate ...	2007	18770621
common risk factors in the development of cataract among arabs residing in kuwait.		2007	1868952
rolling chair sign.		2007	2241609
magnetic resonance imaging in infants exposed to cocaine prenatally: a preliminary report.		2007	1914354
allergic reaction of the oral mucosa in a patient with a synthetic denture.		2007	1868728
fine-structural aspects of bromodeoxyuridine incorporation in sister chromatid differentiation and replication banding.	the structure of harlequin-stained chromosomes following substitution with low levels of 5-bromodeoxyuridine (brdurd) over two cell cycles and high levels over the last part of one cycle (replication banding) was studied in chinese hamster ovary (cho) cells. by using correlative light (lm) and scanning electron microscopy (sem), it was shown that the effects of both the ultraviolet light (u.v.) and hot ssc treatment steps of the harlequin staining procedure were necessary to obtain sister-chroma ...	2008	2621225
epidural morphine, hypertension, and aortic surgery.		2008	2570289
the untreated, shrinking simple bone cavity: case report.		2008	3064707
simple diagnosis of the zellweger syndrome by gas-liquid chromatography of dimethylacetals.	the absence of peroxisomes in patients with the cerebrohepatorenal syndrome of zellweger leads to several biochemical abnormalities, including deficient synthesis of plasmalogens as well as accumulation of very long-chain fatty acids and intermediates in bile acid biosynthesis. accumulation of very long-chain fatty acids in serum and fibroblasts has hitherto been used most extensively for diagnosis. due to the relatively small amounts of the very long-chain fatty acids also in the zellweger pati ...	2008	3760714
standardization of a colorimetric method to quantify growth and metabolic activity of wolbachia-infected mosquito cells.	the aedes albopictus aa23 cell line, which is persistently infected with wolbachia pipientis strain walbb, tends to grow as aggregated clusters of cells that are difficult to disperse for conventional quantification based on cell number. we used a. albopictus c7-10 cells to validate conversion of methylthiazole tetrazolium (mtt) to a colored formazan product with respect to incubation time, cell number over a 40-fold range, and metabolic activity as cells enter stationary phase. using this assay ...	2008	18622662
crystallization and preliminary diffraction analysis of a dsba homologue from wolbachia pipientis.	alpha-dsba1 is one of two dsba homologues encoded by the gram-negative alpha-proteobacterium wolbachia pipientis, an endosymbiont that can behave as a reproductive parasite in insects and as a mutualist in medically important filarial nematodes. the alpha-dsba1 protein is thought to be important for the folding and secretion of wolbachia proteins involved in the induction of reproductive distortions. crystals of native and semet alpha-dsba1 were grown by vapour diffusion and belong to the monocl ...	2008	18259058
cloning, expression, purification and characterization of a dsba-like protein from wolbachia pipientis.	wolbachia pipientis are obligate endosymbionts that infect a wide range of insect and other arthropod species. they act as reproductive parasites by manipulating the host reproduction machinery to enhance their own transmission. this unusual phenotype is thought to be a consequence of the actions of secreted wolbachia proteins that are likely to contain disulfide bonds to stabilize the protein structure. in bacteria, the introduction or isomerization of disulfide bonds in proteins is catalyzed b ...	2008	18387819
molecular and functional characterization of granulin-like molecules of insects.	granulins are a group of highly conserved growth factors that have been described from a variety of organisms spanning the metazoa. here, we report on the identification of two partial transcripts encoding granulin-like molecules from aa23 embryonic cells of aedes albopictus and primary haemocytes from manduca sexta. both these partial transcripts had the characteristic 12-cysteine motif that is a hallmark of the granulin family and they represent the first granulin mrna transcripts identified f ...	2008	18405836
genetic and cytogenetic analysis of the fruit fly rhagoletis cerasi (diptera: tephritidae).	the european cherry fruit fly, rhagoletis cerasi, is a major agricultural pest for which biological, genetic, and cytogenetic information is limited. we report here a cytogenetic analysis of 4 natural greek populations of r. cerasi, all of them infected with the endosymbiotic bacterium wolbachia pipientis. the mitotic karyotype and detailed photographic maps of the salivary gland polytene chromosomes of this pest species are presented here. the mitotic metaphase complement consists of 6 pairs of ...	2008	18545272
genome evolution of wolbachia strain wpip from the culex pipiens group.	the obligate intracellular bacterium wolbachia pipientis strain wpip induces cytoplasmic incompatibility (ci), patterns of crossing sterility, in the culex pipiens group of mosquitoes. the complete sequence is presented of the 1.48-mbp genome of wpip which encodes 1386 coding sequences (cdss), representing the first genome sequence of a b-supergroup wolbachia. comparisons were made with the smaller genomes of wolbachia strains wmel of drosophila melanogaster, an a-supergroup wolbachia that is al ...	2008	18550617
effects of constraint on the oscillatory pattern of endosonic files.	when operated in air, the transverse oscillation of the endosonic file exhibits a series of nodes and antinodes along its length with the greatest displacement amplitude occurring at the unconstrained tip. endosonic files of varying design, length, and thickness exhibit differences in their oscillation. preliminary work has revealed that the endosonic file is susceptible to constraint when working within the root canal and a model system was designed to investigate this problem. the constraining ...	2008	2607288
differential expression of the il-2 receptor subunits, p55 and p75 on various populations of primary peripheral blood mononuclear cells.	unstimulated pbl were examined for expression of il-2r subunits, il-2rp55 and il-2rp75, by two-color flow cytometric analyses using mab. nkh-1+ non-t non-b cells expressed il-2rp75 but not il-2rp55, and the il-2rp75 sites on purified nkh-1+ cells were determined to be 1630 sites/cell by binding of 125i-labeled tu27 mab specific for il-2rp75. in the cd4+ t cell population, il-2rp55+ cells were significantly detected, but little or marginally of the il-2rp75+ cells. however, il-2rp75+ cells were s ...	2008	2584707
[clinical study of osteoporosis].		2008	2614196
ovarian and adrenal function in the parthenogenetic whiptail lizard cnemidophorus uniparens in the field and laboratory.	the purpose of this study was to document circulating concentrations of corticosterone in the plasma in relation to the ovarian follicular cycle in animals in the field and to examine the effects of captivity and laboratory manipulations on plasma corticosterone in the parthenogenetic whiptail lizard cnemidophorus uniparens. in nature, circulating concentrations of corticosterone are highest in previtellogenic individuals when concentrations of progesterone are basal and estradiol-17 beta is und ...	2008	2583474
delirium tremens.		2008	2492848
hyponatraemia in patients with cirrhosis.	hyponatraemia occurs in nearly half of patients in hospital with cirrhosis and ascites, and is due to the excessive retention of free water which results from the kidney's inability to excrete it normally. the morbidity and mortality associated with hyponatraemia is largely attributable to central nervous system disturbances. the degree to which brain water content increases depends on the duration of hyponatraemia and on compensatory mechanisms. altered steroid and peptide hormones in cirrhotic ...	2008	2217672