Publications

spectroscopic studies of the oxidation-reduction properties of three forms of ferredoxin from desulphovibrio gigas.	electron paramagnetic resonance spectra were recorded of three forms of desulphovibrio gigas ferredoxin, fdi, fdi' and fdii. the g = 1.94 signal seen in dithionite-reduced samples is strong in fdi, weaker in fdi' and very small in fdii. the g = 2.02 signal in the oxidized proteins is weak in fdi and strongest in fdii. it is concluded that most of the 4fe-4s centres in fdi change between states c- and c2-; fdi' contain both types of centre. there is no evidence that any particular centre can chan ...	1977	189829
characterization of a novel thiosulfate-forming enzyme isolated from desulfovibrio vulgaris.	an enzyme that formed thiosulfate from bisulfite and trithionate was purified from extracts of desulfovibrio vulgaris. this enzyme, designated as "thiosulfate-forming" enzyme, required the presence of both bisulfite and trithionate. various 35s-labeling studies showed that thiosulfate was formed from bisulfite and the inner sulfur atom of trithionate. this involved a nucleophilic attack by the bisulfite ion, resulting in the displacement of the two outer sulfonate groups of trithionate that recy ...	1977	199572
cytochrome c-551.5 (c7) from desulfuromonas acetoxidans.	cytochrome c-551.5 of the anaerobic sulfur-reducing bacterium desulfuromonas acetoxidans has been purified to homogeneity and characterized. it elicits absorption bands at 551.5, 522.5 and 418 nm in the reduced form; the absorptivity ratio aalpha(red)/a280nm(ox) equals 3.8 for the pure preparation. the molecular weight was estimated to be 9800 by gel filtration. determination of the amion acid composition and analysis of the n-terminal amino acid sequence showed the cytochrome to be identical wi ...	1977	192288
effect of pressure upon the fluorescence of various flavodoxins.	the effects of hydrostatic pressure in the range of 10(-3) to 11 kbar on the fluorescence of flavodoxins from peptostreptococcus elsdenii, desulfovibrio vulgaris, azotobacter vinelandii, and clostridium mp were investigated. the first three flavoproteins showed under high pressure enhancements of flavin fluorescence of over 50 times resulting from the release of flavin mononucleotide from the protein complex. the clostridial flavodoxin showed a very much smaller fluorescence change. at ph 7.5 th ...	1977	20943
nmr characterization of three forms of ferredoxin from desulphovibrio gigas, a sulphate reducer.	a nmr and magnetic susceptibility study of the oxidized and reduced states of three different oligomers (forms) of a [4fe-4s] ferrodoxin protein from desulphovibrio gigas, fdi, fdi', and fdii was carried out. fdi and fdi' are different trimers and fdii a tetramer of the same basic subunit. a probable assignment of the contact shifted resonances is indicated. since the temperature dependences of the contact shifted responances associated with each [4fe-4s] are not all similar a delocalized model ...	1977	836818
phylogenetic studies of two rubredoxins from sulfate reducing bacteria.	the sequences of two rubredoxins isolated from the sulfate reducing bacteria: desulfovibrio vulgaris and desulfovibrio gigas have been elucidated. they have similar sequences but many more differences occur than would be expected from two bacteria of the same genus. of the 52 sites, only 37 are occupied by identical residues. the primary structures are compared with those of the anaerobic bacteria rubredoxins of clostridium pasteurianum, micrococcus aerogenes, pseudomonas oleovorans and peptostr ...	1977	864718
atp and acetylene-reducing activity of a sulfate-reducing bacterium.	a sulfate-reducing bacterium, a strain of desulfovibrio vulgaris, when lactate or pyruvate was supplied as an electron and energy source, reduced acetylene only in the presence of sulfate. acetylene reduction started after a lag of 1 h. h2 which acts as an electron and energy source for sulfate reduction was unable to reduce acetylene even in the presence of sulfate. it was suggested, from the measurements of the amounts of adenine nucleotides of the bacterium incubated under various conditions, ...	1977	871967
evidence for the presence of phosphoriboisomerase and ribulose-1,5-diphosphate carboxylase in extracts of desulfovibrio vulgaris.	cell extracts of desulfovibrio vulgaris were found to incorporate 14co2 into acid-stable products when ribose-5-phosphate or ribulose-1,5-diphosphate was used as a substrate. this co2 fixation required adenosine triphosphate and produced 3-phosphoglyceric acid as one of the products. the assimilation of co2 by pentose phosphates was unrelated to the pyruvate-co2 exchange reaction. the pyruvate-co2 exchange did not require adenosine triphosphate, did not produce phosphorylated compounds, and, unl ...	1977	873881
growth of desulfovibrio in lactate or ethanol media low in sulfate in association with h2-utilizing methanogenic bacteria.	in the analysis of an ethanol-co(2) enrichment of bacteria from an anaerobic sewage digestor, a strain tentatively identified as desulfovibrio vulgaris and an h(2)-utilizing methanogen resembling methanobacterium formicicum were isolated, and they were shown to represent a synergistic association of two bacterial species similar to that previously found between s organism and methanobacterium strain moh isolated from methanobacillus omelianskii. in lowsulfate media, the desulfovibrio produced ac ...	1977	879775
amino acid sequence of desulfovibrio vulgaris flavodoxin.	the complete amino acid sequence for the 148-amino acid flavodoxin from desulfovibrio vulgaris was determined to be: h3n+-met-pro-lys-ala-leu-ile-val-tyr-gly-ser-thr-thr-gly-asn-thr-glu-tyr-thr-ala-glu-thr-ile-ala-arg-glu-leu-ala-asn-ala-gly-tyr-glu-val-asp-ser-arg-asp-ala-ala-ser-val-glu-ala-gly-gly-leu-phe-glu-gly-phe-asp-leu-val-leu-leu-gly-cys-ser-thr-trp-gly-asp-asp-ser-ile-glu-leu-gln-asp-asp-phe-ile-pro-leu-phe-asp-ser-leu-glu-glu-thr-gly-ala-gln-gly-arg-lys-val-ala-cys-phe-gly-cys-gly-as ...	1977	402366
the n-terminal sequence of superoxide dismutase from the strict anaerobe desulfovibrio desulfuricans.		1977	323056
dithionite reduction in the presence of a tetrapyrrole-containing fraction from the desulfoviridin of desulfovibrio gigas.	low-molecular-weight fractions obtained from the desulfoviridin of d. gigas and from the growth medium of desulfovibrio sp. 10455 promoted the reduction of sodium dithionite to sulphide in the presence of reduced methylviologen. these fractions contained a tetrapyrrole of the isobacteriochlorin type which was not complexed with iron, nor was it complexed with protein. the observations are discussed in relation to the function of sulphite reductases in the sulphate-reducing bacteria.	1977	901305
study of two forms of ferredoxin from desulfovibrio gigas by differential pulse polarography.		1977	907681
anaerobic vibrio-like organisms cultured from blood: desulfovibrio desulfuricans and succinivibrio species.	two unusual anaerobic vibrio-like organisms were recovered from blood cultures of two patients. one isolate was identified as desulfovibrio desulfuricans. it appeared to be the cause of a 24-h episode of fever, chills, and profuse perspiration. this is apparently the first documented report of human infection due to this organism. the second isolate was a succinivibrio species. it has rarely been described as a cause of bacteremia. the clinical significance of the organism remains unclear.	1977	858783
bisulfite reductase of desulfovibrio vulgaris: explanation for product formation.	bisulfite reductase, purified from desulfovibrio vulgaris, was coupled with the pyruvate phosphoroclastic reaction. moderate to low reducing conditions resulted in the formation of trithionate; however, when the concentration of reductant was high, a mixture of trithionate and thiosulfate was formed. sulfide was also a detectable product, but only when the concentration of bisulfite was low. flavodoxin mediated native coupling between bisulfite reductase and the phosphoroclastic reaction. a mode ...	1977	914772
growth of sulfate-reducing bacteria with sulfur as electron acceptor.	in addition to three new isolates, six strains of representative species of sulfate-reducing bacteria were tested for their capacity to use elemental sulfur as an electron acceptor for growth. there was good growth and sulfide production by strain norway 4 and the three isolates, two of which had been enriched with sulfur flower and one isolated from a culture with green sulfur bacteria. slow but definite growth was observed with desulfovibrio gigas. the type of strains of desulfovibrio desulfur ...	1977	843165
significance and taxonomic value of iso and anteiso monoenoic fatty acids and branded beta-hydroxy acids in desulfovibrio desulfuricans.	the fatty acids obtained from extractable lipids of the anaerobic sulfate bacterium desulfovibrio desulfuricans were identified. saturated and monoenoic iso (c15-c19) and anteiso (c15, c17) fatty acids and saturated normal (c14-c18) and monoenoic normal (c16, c18) fatty acids were shown to be shown to be present by capillary gas chromatography-mass spectrometry. iso and anteiso beta-hydroxy fatty acids were analyzed as trimethylsilyl ethers in the same way. the position of methyl branches in the ...	1977	845113
the mass spectrometry of iso and anteiso monoenoic fatty acids.		1977	909359
the taxonomy of some new isolates of dissimilatory sulfate-reducing bacteria.	the interrelationships between 92 isolates of sporing and non-sporing sulfate-reducing bacteria were determined. of the 116 biochemical and physiological characteristics examined, only 25 were useful for discrimination of groups. responses to most of the test were negative. a similarity coefficient and a principal component factor analysis of these data were made. the deoxyribonucleic acids buoyant densities (dna) from all strains and the electrophoretic properties of adenylylsulfotransferase (a ...	1977	922597
an iron-containing superoxide dismutase from the strict anaerobe desulfovibrio desulfuricans (norway 4).	superoxide dismutase, the enzyme which catalyzes the dismutation of superoxide free radicals (formula: see text) has been purified to homogeneity from the strict anaerobe sulfate-reducing bacterium desulfovibrio desulfuricans (norway 4). its molecular weight is 43,000 and it is composed of two subunits of equal size which are not covalently bound. the enzyme was found to contain iron by atomic absorption and the absence of acid-labile sulfur indicates that it is not an iron-sulfur protein. elect ...	1977	857918
[participation of sulfate reducing bacteria in copper precipitation].	chemical precipitation of copper by hydrogen sulphide at three values of ph (3.0; 5.0; 7.0) did not result in complete elimination of the metal from a solution. if sulphate reducing bacteria and a source of organic substance, for instance, disintegrated reed, are introduced into the medium, the microorganisms begin to grow, the redox potential decreases, hydrogen sulphide is formed, and copper is completely eliminated from a solution within 10--15 days in model experiments.	1977	859457
isolation and characterization of desulforedoxin, a new type of non-heme iron protein from desulfovibrio gigas.		1977	861023
a structural model of rubredoxin from desulfovibrio vulgaris at 2 a resolution.		1977	881725
[formation and utilization of lactate during methane fermentation of cellulose].	the role of lactate was studied in the course of methane formation from cellulose by a mixed bacterial association. lactate was accumulated in the medium during intensive degradation of cellulose and was assimilated in the period of active gas production. glucose added to the medium was converted into lactate within 30 min. if lactate served as a substrate for a methane forming association which was previously cultivated in a medium containing cellulose, it was almost completely converted into m ...	1978	745555
the discovery of nature's biosynthetic pathways.	uro'gen-iii is a key intermediate on the biosynthetic pathways to the vitally important natural pigments haem, chlorophyll and the cytochromes. how the unexpected structure of uro'gen-iii is synthesized by living things has long been a major puzzle. studies based on 13c-labelling are described which show a) that a single intramolecular rearrangement occurs and b) that this step occurs after the open-chain linear tetrapyrrole system has been built. a second study involves stereospecific labelling ...	1978	620714
the role of dihydrogen and hydrogenase in nitrogen fixation.		1978	667177
inhibition of methanogenesis by sulphate reducing bacteria competing for transferred hydrogen.	a methanogenic bacterial consortium was obtained after inoculation of benzoate medium under n2/co2 atmosphere with intertidal sediment. a hydrogen donating organotroph and methanococcus mazei were isolated from this enrichment. h2-utilising sulphate reducing bacteria were isolated under h2/co2 in the absence of organic electron donors. the methanococcus was able to produce methane in yeast extract medium under n2/co2 if the h2 donating organism was present, and sulphate reduction occurred if the ...	1978	678014
dissimilatory reduction of bisulfite by desulfovibrio vulgaris.	the reduction of bisulfite by desulfovibrio vulgaris was investigated. crude extracts reduced bisulfite to sulfide without the formation (detection) of any intermediates such as trithionate or thiosulfate. when the particulate fractions was removed from crude extracts by high-speed centrifugation, the soluble supernatant fraction reduced bisulfite sequentially to trithionate, thiosulfate, and sulfide. addition of particles or purified membranes to the soluble fraction restored the original activ ...	1978	721780
hydrogen as a substrate for methanogenesis and sulphate reduction in anaerobic saltmarsh sediment.	hydrogen gas stimulated sulphate reduction in a saltmarsh sediment and the importance of h2 transferred from organotrophic bacteria to the sulphate-reducers is discussed. beta-fluorolactate inhibited sulphate reduction whether lactate, ethanol or hydrogen was being used as growth substrate. when added to sediment beta-fluorolactate inhibited sulphate reduction with a consequent increase in methane production. addition of h2 stimulated methanogenesis in sediment and this stimulation was greater i ...	1978	678015
a chemiosmotic model for sulphate respiration.		1978	720591
electron-transfer mechanisms in multihaem cytochromes [proceedings].		1978	744410
a comparative spectroscopic study of two non-haem iron proteins lacking labile sulphide from desulphovibrio gigas.	the ultraviolet visible, and near infrared spectrum of a two-iron protein from desulphovibrio gigas, a new type of non-haem iron protein lacking labile sulphide, is compared with that of d. gigas rubredoxin. the charge transfer band maxima of rubredoxin at 495 and 565 nm are less separated in the new protein implying a higher symmetry of the two iron centres. the existence of a spin-spin interaction between the two iron centres in the new protein is suggested by the magnetic susceptibility measu ...	1978	205263
reactivity of desulfovibrio gigas hydrogenase toward artificial and natural electron donors or acceptors.	a purified preparation of hydrogenase from d. gigas was inactive toward ferredoxin, flavodoxin or rubredoxin in the absence of cytochrome c3 (m.w. 13,000), in an atmosphere of hydrogen, although direct reduction of benzyl viologen or fmn was possible. the hydrogen evolution reaction from dithionite was possible with methyl viologen. the same reaction also occured with cytochrome c3 (m.w. 13,000) or cytochrome c3 (m.w. 26,000). addition of either ferredoxin or flavodoxin did not accelerate the re ...	1978	667185
studies on the reaction of imidazole with cytochrome c3 from desulfovibrio vulgaris.		1978	26395
separation of hydrogenase from intact cells of desulfovibrio vulgaris. purification and properties.		1978	620819
isolation and characterization of desulfovibrio growing on hydrogen plus sulfate as the sole energy source.		1978	623496
structural control of the redox potentials and of the physiological activity by oligomerization of ferredoxin.		1978	658398
hydrogen bonding of flavoprotein. i. effect of hydrogen bonding on electronic spectra of flavoprotein.	the effect of hydrogen bonding on the transition energy and the oscillator strength of the isoalloxazine nucleus of flavins was studied by the molecular orbital method. among the possible hydrogen bondings examined, characteristic spectral shifts were found for the hydrogen bondings at n(1) and n(5) of the nucleus. the hydrogen bonding at n(1) resulted in the shift of the first absorption band towards blue and that of the second one towards red. on the other hand, the hydrogen bonding at n(5) re ...	1978	28779
[staining of sulfate-reducing bacteria with hexacyanoferrat-compounds (cultural and cytochemical evidence of fe) (author's transl)].	hexacyanoferrat-compounds are suitable 1. to indicate the valence of fe-ions in cultures of desulfuricants (desulfovibrio spec., desulfotomaculum) in fluid and solid medium before and after inoculation; 2. stain directly the sulfate-reducing bacteria in presence of fe-ions and give a colour-reaction of those parts of the cell containing fe-compounds, e.g. fes. therefore they act as a cytochemical indicator system. the chemical base of the reactions are as follows: (1) 4hs + so42 leads to h2s + 2 ...	1978	77605
kinetics of reduction of the cytochrome c3 from desulvovibrio vulgaris.		1978	214075
oxidation-reduction studies of the mo-(2fe-2s) protein from desulfovibrio gigas.	potentiometric titration followed by e.p.r. measurements were used to determine the midpoint reduction potentials of the redox centres of a molybdenum-containing iron-sulphur protein previously isolated from desulfovibrio gigas, a sulphate-reducing bacterium (moura, xavier, bruschi, le gall, hall & cammack (1976) biochem. biophys. res. commun. 728 782-789; moura, xavier, bruschi, le gall & cabral (1977) j. less common metals 54, 555-562). the iron-sulphur centres could readily be distinguished i ...	1978	212012
nmr studies of electron carrier proteins from sulphate reducing bacteria.	the sulphate-reducing bacteria have a complex electron transfer system which leads to the reduction of sulphate by oxidation of either organic substrates or molecular hydrogen. these bacteria can either produce or consume molecular hydrogen. the central part of this electron pathway for desulovibrio gigas is constituted by hydrogenase (3 x (4fe-4s)). cytochrome c3 (4 haems with different redox potentials) and a one (4fe-4s) cluster ferredoxin. this ferredoxin is isolated in different oligomeric ...	1978	208661
epr determination of the oxidation-reduction potentials of the hemes in cytochrome c3 from desulfovibrio vulgaris.	epr spectroscopy in conjunction with oxidation-reduction potentiometry has been used to determine the half-reduction potentials of the four hemes of cytochrome c3. as predicted, the four hemes of cytochrome c3 have different mid-point potentials. the em values are: heme i,--284 mv; heme ii,--310 mv; heme iii,--324 mv and heme iv,--319 mv. the n-values in each case was near one.	1978	208660
characterization of the periplasmic hydrogenase from desulfovibrio gigas.		1978	208565
rapid sampling culture chamber.	an all-glass chamber for culturing anaerobic and aerobic bacteria in liquid medium is described. the system permits both rapid sampling and turbidimetric measurements under controlled atmospheric conditions.	1978	350159
dimethyl sulfoxide as an electron acceptor for anaerobic growth.	the isolation from lake mud of a bacterium which can use dimethyl sulfoxide (dmso) as an electron acceptor for growth is described. the isolate, called strain dl-1, was a small, gram negative, non-motile spiral. the sole product of dmso reduction was dimethyl sulfide (dms). other electron acceptors used by the isolate included sulfite, thiosulfate, elemental sulfur, methionine sulfoxide, tetramethylene sulfoxide, nitrate, and oxygen (microaerophilically). sulfate was not reduced and could not ev ...	1978	414686
occurrence of glutathione in bacteria.	glutathione and soluble thiol content were examined in a broad spectrum of bacteria. significant soluble thiol was present in all cases. the thiol compound was glutathione in most of the gram-negative bacteria but not in most of the gram-positive bacteria studied. glutathione was absent in four anerobes and one microaerophile but was present in a blue-green bacterium. the glutathione content of escherichia coli increased significantly during transition from exponential to stationary phase.	1978	417060
growth yields and growth rates of desulfovibrio vulgaris (marburg) growing on hydrogen plus sulfate and hydrogen plus thiosulfate as the sole energy sources.		1978	28099
physiological and morphological observations on thiovulum sp.	cell suspensions of thiovulum sp., collected from enrichment cultures, were grown, maintained, and harvested for periods up to 7 months. in open-flow cultures run with aerated seawater, a continuous supply of hydrogen sulfide was provided by diffusion through a semipermeable membrane from either a live culture of desulfovibrio esturaii, neutralized sodium sulfide, or a n2-h2s gas mixture. attempts to grow thiovulum in pure culture failed despite variation in concentrations of dissolved oxygen an ...	1978	101531
siroheme: methods of isolation and characterization.		1978	149890
structural homology of cytochromes c.	cytochromes c from many eukaryotic and diverse prokaryotic organisms have been investigated and compared using high-resolution nuclear magnetic resonance spectroscopy. resonances have been assigned to a large number of specific groups, mostly in the immediate environment of the heme. this information, together with sequence data, has allowed a comparison of the heme environment and protein conformation for these cytochromes. all mitochondrial cytochromes c are found to be very similar to the cyt ...	1978	203462
rubredoxin.		1978	213684
purification of electron-transfer components from sulfate-reducing bacteria.		1978	213690
purification and some properties of cytochrome c553(550) isolated from desulfovibrio desulfuricans norway.		1979	219858
electrochemistry of cytochrome c3 from desulfovibrio desulphuricans (norway) [proceedings].		1979	225230
purification and properties of cytochrome c-553, an electron acceptor for formate dehydrogenase of desulfovibrio vulgaris, miyazaki.	cytochrome c-553 of desulfovibrio vulgaris, miyazaki, was purified to homogeneity. the absorption spectrum of the ferro form has four peaks at 553, 525, 417 and 317 nm with a plateau near 280 nm, and that of the ferri form has three peaks at 525, 410 and 360 nm with a plateau near 280 nm and a shoulder at 560 nm. the millimolar absorbance coefficient of the alpha-peak of the ferro form is 23.9. the molecular weight of cytochrome c-553 is 8000, and it contains one heme. its isoelectric point is r ...	1979	226135
oxidation-reduction potentials of the hemes in cytochrome c3 from desulfovibrio gigas in the presence and absence of ferredoxin by epr spectroscopy.	1. ferricytochrome c3 from d. gigas exhibits two low-spin ferric heme epr resonances with gz-values at 2.959 and 2.853. ferrocytochrome c3 is diamagnetic based on the absence of any epr signals. 2. epr potentiometric titrations result in the resolution of the two low-spin ferric heme resonances into two additional heme components representing in total the four hemes of the cytochrome, with em values of -235 mv and -315 mv at heme resonance i and em values of -235 mv and -306 mv at heme resonance ...	1979	227477
[hexacyanoferrate-compounds as indicator substances for the efficacy of antimicrobial agents to desulfuricants (author's transl)].	hexacyanoferrate-compounds are suitable to indicate the efficacy of antimicrobial substances to sulfate-reducing bacteria. they act as a cytochemical indicator system. (stübner, 9), e. g. k3feiii(cn)6 showes fes formation before any visible blackening of the medium. by using hexacyanoferrate compounds it is easier to determine the efficacy of antimicrobial agents. in addition a microscopical control is recommended to detect damaged or avital bacteria in the limiting concentrations of the agents.	1979	44792
study on dissimilatory reduction of sulphates.	a new strain of sulphate reducing bacteria was isolated from swampy forest soil. after 120 h reduction, sulphate conversion attained 100%, the molar ratio of the consumed lactate and reduced sulphate amounted to 2:1. this confirms the reduction mechanism proposed by senez (1951).	1979	87119
the effect of organic substrate concentration on activity for microbiological reduction of sulfates.	lactate, pyruvate, malate and alpha-glycerophosphate are excellent sources of energy and structural carbon for desulfovibrio vulgaris. a fully stoichiometric course of the sulfate reduction process has been observed. a simplified equation of the reaction describing the respiration process of the above bacteria in the presence of alpha-glycerophosphate is proposed.	1979	92173
[fixation of molecular nitrogen by sulfate-reducing bacteria from petroleum strata].	the activity of nitrogen fixation by the museum strains of sulfate reducing bacteria isolated from oil deposits was studied using the acetylene technique. the mesophilic sulpfate reducing bacteria belonging to the species desulfovibrio africanus 2372 and d. baculatus x were found to have a high nitrogenase activity. d desulfuricans subsp. aestuarii 2198 reduced acetylene at a low rate. the thermophilic sulfate reducing cultures of d. thermophilus 7, desulfotomaculum nigrificans 781 and dm. nigri ...	1979	423802
isolation and characterization of a molybdenum iron-sulfur protein from desulfovibrio africanus.		1979	426817
the interaction of polymeric viologens with hydrogenases from desulfovibrio desulfuricans and clostridium pasteurianum.	the interaction between hydrogenases from either desulfovibrio desulfuricans or clostridium pasteurianum and electron donors methyl viologen or polymeric viologens was examined. extracts from each organism contained a single gel electophoretic band of active hydrogenase. the hydrogenase of d. desulfuricans was much more stable than that of cl. pasteurianum. with methyl viologen apparent km and vm values were 0.5 mm and 0.62 mumole h2/min per milligram protein for the cl. pasteurianum and 0.7 and ...	1979	396014
the amino acid sequence of desulforedoxin, a new type of non heme iron protein from desulfovibrio gigas.		1979	508323
[distribution of thermophilic sulfate reducing bacteria in the oil-bearing strata of apsheron and western siberia].	enrichment cultures of sulfate-reducing bacteria were obtained by inoculating the water of high-temperature gas and oil-bearing strata. a study of the morphology of these cells has shown that the thermophilic bacterium desulfovibrio thermophilus occurs in the deposits of the apsheron peninsula while desulfootomaculum nigrificans is found in the deposits of west siberia. the former organism is involved in the accumulation of h2s in the apsheron strata. the temperature of the strata is believed to ...	1979	530138
sulfide production by some rumen bacteria.		1979	533168
transformation of mercuric chloride and methylmercury by the rumen microflora.	the microflora in strained rumen fluid did not methylate or volatilize 203hg2+ at detectable rates. however, there was an exponential decay in the concentration of added ch3hg+, which was attributed to demethylation. the major product of demethylation was metallic mercury (hg0), and it was released as a volatile product from the reaction mixture. demethylation occurred under both anaerobic and aerobic conditions. the rate of demethylation was proportional to the concentration of added ch3hg+-hg ...	1979	539820
epr spectroscopy of the iron-sulphur cluster and sirohaem in the dissimilatory sulphite reductase (desulphoviridin) from desulphovibrio gigas.	desulphoviridin in the oxidized state showed epr signals around g = 6, consistent with the sirohaem being in the high-spin ferric state. this was unreactive with sulphite, sulphide or cyanide; but readily reduced by methyl viologen. when the enzyme was treated with na2s2o4 the sirohaem was slowly reduced and a spectrum of a reduced iron-sulphur cluster at g = 2.07, 1.93, 1.91 appeared over the course of an hour. an intermediate in this reaction was indicated by a free radical signal which appear ...	1979	228747
redox studies on rubredoxins from sulphate and sulphur reducing bacteria.		1979	228978
hydrogenase activity in the dry state: isotope exchange and reversible oxidoreduction of cytochrome c3.	hydrogenase (hydrogen:ferricytochrome c3 oxidoreductase, ec 1.12.2.1) catalyzes three types of reactions, i.e., (1) conversion between hydrogen modifications, para-h2 and ortho-h2, (2) exchange reaction between hydrogen isotopes, and (3) reversible oxidoreduction of an electron carrier with h2 and protons. we observed that purified desulfovibrio hydrogenase in the dry state could catalyze not only the conversion and exchange reactions (yagi, t., tsuda, m., mori, y. and inokuchi, h. (1969) j. am. ...	1979	222328
structure and sequence of the multihaem cytochrome c3.	the molecular structure of cytochrome c3 isolated from desulfovibrio desulfuricans has been solved on the basis of its crystallographic determination at 2.5 a resolution and of an essentially complete sequence. the molecule consists of a single polypeptide chain wrapped around a very compact core of four non-parallel haems which present a relatively high degree of exposure to the solvent. alignment of the amino acid sequences of cytochrome c3 from several species suggests that the structure repo ...	1979	229424
structure-function relationship in hemoproteins: the role of cytochrome c3 in the reduction of colloidal sulfur by sulfate-reducing bacteria.	cytochromes c3 of different strains of sulfate-reducing bacteria have been purified and tested for their capacity to reduce colloidal sulfur to hydrogen sulfide. the results are in good agreement with the activities reported for the whole cells. cytochrome c3 is the sulfur reductase of some strains of sulfate-reducing bacteria such as desulfovibrio desulfuricans norway 4 and sulfate-reducing bacterium strain 9974 from which the sulfur reductase activity can be purified with the cytochrome c3. in ...	1979	229785
cytochrome c3 from the sulfate-reducing anaerobe desulfovibrio africanus benghazi: purification and properties.	cytochrome c3 was purified from desulfovibrio africanus benghazi by extraction with alkaline deoxyribonuclease, fractionation with ammonium sulfate, batch elution from carboxymethyl sephadex followed by chromatography on the same resin, and gel filtration on sephadex g-75. the preparation was judge homogeneous by a variety of criteria. the molecular weight was determined in an analytical ultracentrifuge, and values between 14,400 and 15,490 were obtained, depending upon the presumed value of par ...	1979	230179
study of cytochromes c3 from desulfovibrio vulgaris (hildenborough) and desulfovibrio desulfuricans (norway) by differential pulse polarography and spectroelectrochemical method.	(1) cytochromes c3 from desulfovibrio vulgaris (hildenborough strain) and desulfovibrio desulfuricans (norway strain) have been studied by differential pulse polarography and spectroelectrochemical method. both cytochromes exhibit two reduction potential values eh approximately --0.25 and --0.34 v (d. vulgaris), eh approximately --0.16 and --0.34 v (d. desulfuricans). (2) titrations by dithionite and controlled potential electrolysis coupled with polarography and spectrophotometry suggest that i ...	1979	758940
solar-driven chemical energy source for a martian biota.	microorganisms deep in the martian soil could derive energy indirectly from the sun via chemical reactions involving atmospheric products of the solar ultraviolet flux. the viking discovery of a chemically uniform regolith which, though poor in organics, is rich in sulfur-containing compounds suggests reaction sequences in which sulfur is recycled through reduced and oxidized states by biologically catalyzed reactions with photochemically-produced atmospheric constituents. one candidate reaction ...	1979	481874
isolation and characterization of a rubredoxin and two ferredoxins from desulfovibrio africanus.	rubredoxin and two distinct ferredoxins have been purified from desulfovibrio africanus. the rubredoxin has a molecular weight of 6000 while the ferredoxins appear to be dimers of identical subunits of approximately 6000 to 7000 molecular weight. rubredoxin contains one iron atom, no acid-labile sulfide and four cysteine residues per molecule. its absorbance ratio a278/a490 is 2.23 and its amino acid composition is characterized by the absence of leucine and a preponderance of acidic amino acids ...	1979	508735
amino acid sequence of desulfovibrio gigas ferredoxin: revisions.		1979	518659
on the redox properties of three bisulfite reductases from the sulfate-reducing bacteria.		1979	230837
oxidative phosphorylation linked to the dissimilatory reduction of elemental sulphur by desulfovibrio.	hydrogenase and cytochrome c3 purified from desulfovibrio gigas and d. desulfuricans strain norway form a soluble complex which is capable of transferring electrons from molecular hydrogen to colloidal sulphur (s0). in this reaction, sulphur is reduced to hydrogen sulphide. since both strains are capable of growth using elemental sulphur as terminal electron acceptor, it was of interest to check for oxidative phosphorylation in this sulphur reduction sytem. membranes isolated from d. gigas or d. ...	1979	232998
biochemical studies on sulfate-reducing bacteria. xv. separation and comparison of two forms of desulfoviridin.	desulfoviridin from desulfovibrio vulgaris was separated into two forms by deae-sephadex column chromatography. the major form had a pi of 4.4 and the minor form one of 4.5-4.6. both forms produced mainly trithionate, besides thiosulfate and sulfide, in methylviologen-linked sulfite reduction. the specific activities of sulfite reduction, as well as of hydroxylamine reduction, were virtually identical in both forms. there were no great differences in their absorption spectra, cd spectra, molecul ...	1979	429261
crystallographic data for cytochrome c3 from two strains of desulfovibrio vulgaris, miyazaki.	two crystalline forms of cytochrome c3 isolated from two strains of desulfovibrio vulgaris, miyazaki, tentatively designated as d. vulgaris, miyazki f and d. vulgaris, miyazaki k, have been found. both belong to the orthorhombic system, space group p2(1)2(1)2(1), but have different cell dimensions; a=54.1, b=68.9 and c=35.0 a for d. vulgaris, miyazaki f, and a=43.5, b=41.2, and c=62.9 a for d. vulgaris, miyazaki k. the asymmetric unit of each crystal contains one molecule of cytochrome c3.	1979	479126
catalytic activity of the chromophore of desulfoviridin, sirohydrochlorin, in sulfite reduction in the presence of iron.	sirohydrochlorin chromophore prepared by acetone/hcl treatment of desulfoviridin, the sulfite reductase from desulfovibrio, catalyzed the reduction of sulfite to sulfide and thiosulfate in equimolar amounts when coupled with a hydrogen-hydrogenase-methyl viologen system. this activity was manifested at acidic ph and increased exponentially with decrease in ph. the km value for sulfite was nearly 10 times that of desulfoviridin. inorganic iron was necessary for the reduction, since inactivation o ...	1979	479127
restriction endonucleases and their recognition sequences.		1980	6249680
on the nature of the oxidation-reduction properties of nitrite reductase from desulfovibrio desulfuricans.		1980	6254507
a cobalt containing protein isolated from desulfovibrio gigas, a sulfate reducer.		1980	6244822
amino acid sequence of cytochrome c3 from desulfovibrio vulgaris, miyazaki.	the complete amino acid sequence of a tetrahemoprotein, cytochrome c3 isolated from desulfovibrio vulgaris, miyazaki, was determined to be ala-pro-lys-ala-pro-ala-asp-gly-leu-lys-met-asp-lys-thr-lys-gln-pro-val-val-phe -asn-his-ser-thr-his-lys-ala-val-lys-cys-gly-asp-cys-his-his-pro-val-asn-gly-lys-glu-asn-tyr-gln-lys-cys-ala-thr-ala-gly-cys-his-asp-asn-met-asp-lys-lys-asp-lys-ser-ala-lys-gly-tyr-tyr-his-ala-met-his-asp-lys-gly-thr-lys-phe-lys-ser-cys-val-gly-cys-his-leu-glu-thr-ala-gly-ala-asp- ...	1980	6249799
a new restriction endonuclease from the anaerobic bacterium, desulfovibrio desulfuricans, norway.	the purification and characterization of a new restriction endonuclease, dde 1, from a sulfate-reducing, anaerobic bacterium, desulfovibrio desulfuricans, norway, is reported. the enzyme recognizes the sequence (see formula index) and cleaves at the position indicated by the arrows. the enzyme preparation obtained is suitable for restriction mapping an ligation.	1980	6255409
mössbauer and epr studies of desulforedoxin from desulfovibrio gigas.		1980	6244281
highly active immobilized hydrogenase from desulfovibrio gigas.		1980	6245644
serological characteristics within the genus desulfovibrio.	antisera were prepared against one strain each of desulfovibrio desulfuricans, d. vulgaris and d. salexigens. the antisera were tested for cross reactivity against 36 heterologous desulfovibrio strains by both agglutination titration and by double immunodiffusion percipitin plates. generally no cross-reaction was demonstrated by agglutination even between heterologous strains of the same species, suggesting that the surface antigens of desulfovibrio are highly specific. in immunodiffusion plates ...	1980	6772102
the anaerobic dissimilation of benzoate by pseudomonas aeruginosa coupled with desulfovibrio vulgaris, with sulphate as terminal electron acceptor.		1980	6778744
evidence for a three-iron center in a ferredoxin from desulfovibrio gigas. mössbauer and epr studies.	the tetrameric form of a desulfovibrio gigas ferredoxin, named fd ii, mediates electron transfer between cytochrome c3 and sulfite reductase. we have studied two stable oxidation states of this protein with mössbauer spectroscopy and electron paramagnetic resonance. we found 3 iron atoms/monomer and a spin concentration of 0.9 spins/monomer for the oxidized protein. taken together, the epr and mössbauer data demonstrate conclusively the presence of a spin-coupled structure containing 3 iron atom ...	1980	6245073
[thiosulfate as an intermediate product of bacterial sulfate reduction].	sulfur compounds produced at intermediate stages during transformation of sulfate to sulfide were analyzed in experiments with a culture of sulfate reducing bacteria. small quantities of thiosulfate can accumulate in the medium at the beginning of growth of the sulfate reducing bacterium. the data are discussed and compared with the results of chambers and trudinger (1975) who could not detect thiosulfate in similar experiments.	1980	7207258
desulforedoxin: preliminary x-ray diffraction study of a new iron-containing protein.		1980	7253026
redox reactions of the hydrogenase-cytochrome c3 system from desulfovibrio gigas with the synthetic analogue of ferredoxin active site [fe4s4 (s-ch2-ch2oh)4]2-.		1980	7370033
the association of hydrogenase and dithionite reductase activities with the nitrite reductase of desulfovibrio desulfuricans.		1980	7387702
purification of carbon monoxide dehydrogenase, a nickel enzyme from clostridium thermocaceticum.	carbon monoxide dehydrogenase (co dehydrogenase) has been purified from the homoacetate-fermenting bacterium, clostridium thermoaceticum. by use of 63ni, it has been determined that the dehydrogenase is a metallo nickel enzyme. nickel was rapidly taken up by the organism and most of the ingested metal was found to be incorporated into co dehydrogenase. as estimated by gel filtration, the native enzyme has a molecular weight of 410,000. ferredoxin and a membrane-bound b-type cytochrome, both obt ...	1980	6893049
factors affecting the production of hydrogenase by desulfovibrio desulfuricans.	an examination of conditions for the growth of desulfovibrio desulfuricans, with the aim of optimizing hydrogenase production, is reported. an ammonium sulfate-lactate-yeast extract medium gave 5 to 10 times as much hydrogenase activity as a peptone-yeast extract medium. it made little if any difference whether the gas used for sparging was nitrogen, hydrogen, or a mixture thereof but increasing the rate of sparging and agitation did result in a slight decrease in activity. control of ph during ...	1980	7006764
purification and properties of the periplasmic hydrogenase from desulfovibrio desulfuricans.	the periplasmic hydrogenase of desulfovibrio desulfuricans was isolated and purified. cells were washed with tris-edta and the enzyme precipitated from the wash with ammonium sulfate. absorption chromatography on deae and hydroxyapatite yielded the enzyme at better than 95% purity as judged by gel electrophoresis. the hydrogenase catalyzed the production of more than 9000 mumol h2/min mg protein(-1) from reduced methyl viologen at 37 degrees c. it is very stable and resists inactivation by heat ...	1980	7006765
cysteine synthesis by desulfovibrio vulgaris extracts.	extracts of desulfovibrio vulgaris were found to contain serine transacetylase and cysteine synthase activities. when extracts were incubated with bisulfite and o-acetylserine, or acetyl coenzyme a plus l-serine, under a hydrogen atmosphere, cysteine was formed. pyruvate served as a reductant for bisulfite reduction to sulfide and concomitantly provided the acetyl moiety for acetyl coenzyme a formation. consequently, when extracts were incubated with pyruvate, bisulfite, and l-serine, cysteine s ...	1980	7364731