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[clinical criteria for evaluation of hemophilia severity in expert estimation of patient's working capacity].data are presented on the evaluation of hemophilia severity during expert estimation of the patient's working capacity according to the information of the republican centre. the authors have developed the most optimal classification for the evaluation of the disease severity; the influence of hemorrhagic symptoms and concomitant diseases on hemophilia course and prognosis has been considered.20061426924
[differential diagnosis of 2 forms of necrospermia]. 20063321663
antigen-receptor complex stimulation triggers protein kinase c-dependent cd11a/cd18-cytoskeleton association in t lymphocytes.although it is well accepted that intercellular adhesion involving the cd11a/cd18 (lfa-1) complex is critical in a wide array of t cell-dependent processes, recent demonstrations of an lfa-1 high avidity state, induced by triggering the t cell receptor (tcr) complex, has raised questions about the intracellular signals generated and molecular events leading to effective cell coupling, as well as their orderly sequence. in this study, we assessed the effects of t cell activation on the actin-base ...20061346786
chemotherapy-related hemolytic-uremic syndrome after the treatment of head and neck cancer. a case report.a 62-year-old woman who was being treated for squamous cell carcinoma of the head and neck developed a chemotherapy-related hemolytic-uremic syndrome during the second cycle of neoadjuvant chemotherapy consisting of cisplatin, bleomycin, and methotrexate. though the syndrome was suspected early, attempts at reversing the hematologic and renal abnormalities were unsuccessful. at postmortem examination, the characteristic microvascular lesions of the hemolytic-uremic syndrome were found in the kid ...20072224786
insulin secretagogues induce ca(2+)-like changes in cytoplasmic mg2+ in pancreatic beta-cells.the effects of insulin secretagogues on the cytoplasmic mg2+ concentration ([mg2+]i) of pancreatic beta-cells were studied in suspensions and in individual beta-cells using dual-wavelength fluorometry and the indicator mag-fura-2. average [mg2+]i was in the 800-900 microm range in a medium containing 3 mm glucose. when the sugar concentration was raised to 20 mm, the cells reacted with an initial lowering of [mg2+]i followed by an increase. the sugar apparently also stimulated leakage of the mg2 ...20072223873
insulin secretagogues induce ca(2+)-like changes in cytoplasmic mg2+ in pancreatic beta-cells.the effects of insulin secretagogues on the cytoplasmic mg2+ concentration ([mg2+]i) of pancreatic beta-cells were studied in suspensions and in individual beta-cells using dual-wavelength fluorometry and the indicator mag-fura-2. average [mg2+]i was in the 800-900 microm range in a medium containing 3 mm glucose. when the sugar concentration was raised to 20 mm, the cells reacted with an initial lowering of [mg2+]i followed by an increase. the sugar apparently also stimulated leakage of the mg2 ...20072223873
[delirious and hallucinatory disorders in late psychoses with the pattern of "home paranoia"].paranoid and hallucination syndromes were studied in 71 cases of senile non-dementia psychoses located at the patients' house. two major variants were singled out: paranoid and hallucinatory (the latter subdivided into patterns with or without the persecution delusion, and physical affection delusion). paranoid variant was shown to be based upon a special transformation of visual perception with formation of delusions of penetration of a "stranger" into the patients' home. hallucinatory variants ...20072176045
a new manual method for assessing two-dimensional cancellous bone structure: comparison between iliac crest and lumbar vertebra.we have developed a simple method for the direct analysis of two-dimensional trabecular structure. using this method, we have compared the structure of the left and right iliac crest and the second lumbar vertebra and studied the relationship between two-dimensional trabecular structure and the ultimate compressive stress of bone samples from the second lumbar vertebra. transverse cores were taken from the left and right iliac crests and second lumbar vertebrae of 35 subjects (12 females) who di ...20071950674
body mass index variations: centiles from birth to 87 years.this report provides body mass index (weight/height2) values for the french population from birth to the age of 87 years. bmi curves increase during the first year, decrease until the age of 6, increase again up to 65 years and decrease thereafter. these variations reflect the total changes of fat body mass during life. the 50th centile values of wt/ht2 at the ages of 20, 40, 60, 80 years are 21.5, 24.6, 25.4, 24.4 kg/m2 for men and 20.6, 22.6, 24.1, 23.4 kg/m2 for women. the values for the 3rd, ...20071855495
time to adoption of an innovation by dentists in private practice: sealant utilization.dentists can be divided into five adoption categories based upon their time of adoption of pit and fissure sealants: innovators, early adopters, early majority, late majority, and laggards. the differences among dentists in the five adoption categories were examined for four classes of variables: practice characteristics, dentist characteristics, communication of information, and practice environment. questionnaires were mailed in september 1984 to a random sample (n = 521) of washington state d ...20071920266
admissions to mental handicap hospitals. 20071932957
sleep and sexual function in the elderly male. 20071892957
rna polymerase beta-subunit gene (rpob) sequence analysis for the identification of bacteroides spp.partial rpob sequences (317 bp) of 11 species of bacteroides, two porphyromonas spp. and two prevotella spp. were compared to delineate the genetic relationships among bacteroides and closely related anaerobic species. the high level of inter-species sequence dissimilarities (7.6-20.8%) allowed the various bacteroides spp. to be distinguished. the position of the bacteroides distasonis and bacteriodes merdae cluster in the rpob tree was different from the position in the 16s rrna gene tree. base ...200717184287
antianaerobe activity of ceftobiprole, a new broad-spectrum cephalosporin.agar dilution testing of 463 anaerobes showed most gram-positive beta-lactamase-negative strains (other than some clostridium difficile and peptostreptococcus anaerobius), as well as both beta-lactamase-positive and beta-lactamase-negative strains of fusobacterium nucleatum, to have ceftobiprole mic values of < or =0.016 to 4 microg/ml. ceftobiprole was less active against beta-lactamase-positive gram-negative bacilli, especially the members of the bacteroides fragilis group. like ceftobiprole, ...200717240107
barnesiella viscericola gen. nov., sp. nov., a novel member of the family porphyromonadaceae isolated from chicken caecum.two bacterial strains isolated from chicken caecum, c46t and c47, were characterized using a polyphasic taxonomic approach that included analysis of the phenotypic and biochemical features, cellular fatty acid profiles, menaquinone profiles and phylogenetic position (using 16s rrna gene sequence analysis). the 16s rrna gene sequence analysis showed that these strains belonged to the family porphyromonadaceae. these strains shared 100 % 16s rrna gene sequence similarity with each other and were r ...200717267976
phase-variable expression of a family of glycoproteins imparts a dynamic surface to a symbiont in its human intestinal ecosystem.the recent report of the synthesis of glycoproteins by the abundant intestinal symbionts bacteroides showed that these organisms use a novel bacterial enzyme to decorate their surfaces with a sugar residue derived from their environment. as a first step in understanding the importance of these glycoproteins to the bacteria and to the bacterial-host symbiosis, we identified and characterized the abundant glycoproteins of bacteroides distasonis (proposed reclassification as parabacteroides distaso ...200717284602
luminex detection of fecal indicators in river samples, marine recreational water, and beach sand.research to understand and remediate coastal pollution is moving toward a multitiered approach in which traditional enumeration of fecal indicators is accompanied by molecular analysis of a variety of targets. technology that rapidly detects multiple microbial contaminants would benefit from such an approach. the luminex 100 system is a suspension array that assays multiple analytes rapidly in a single well of a microtiter plate. the ability of the system to simultaneously detect multiple fecal ...200717350051
identification of commensal bacterial strains that modulate yersinia enterocolitica and dextran sodium sulfate-induced inflammatory responses: implications for the development of probiotics.an increasing body of evidence suggests that probiotic bacteria are effective in the treatment of enteric infections, although the molecular basis of this activity remains elusive. to identify putative probiotics, we tested commensal bacteria in terms of their toxicity, invasiveness, inhibition of yersinia-induced inflammation in vitro and in vivo, and modulation of dextran sodium sulfate (dss)-induced colitis in mice. the commensal bacteria escherichia coli, bifidobacterium adolescentis, bacter ...200717485456
association between the cfxa gene and transposon tn4555 in bacteroides distasonis strains and other bacteroides species.the bacteroides genus, the most prevalent anaerobic bacteria of the intestinal tract, carries a plethora of the mobile elements, such as plasmids and conjugative and mobilizable transposons, which are probably responsible for the spreading of resistance genes. production of beta-lactamases is the most important resistance mechanism including cephalosporin resistance to beta-lactam agents in species of the bacteroides fragilis group. in our previous study, the cfxa gene was detected in b. distaso ...200717486409
evolution of symbiotic bacteria in the distal human intestine.the adult human intestine contains trillions of bacteria, representing hundreds of species and thousands of subspecies. little is known about the selective pressures that have shaped and are shaping this community's component species, which are dominated by members of the bacteroidetes and firmicutes divisions. to examine how the intestinal environment affects microbial genome evolution, we have sequenced the genomes of two members of the normal distal human gut microbiota, bacteroides vulgatus ...200717579514
segmented filamentous bacteria in a defined bacterial cocktail induce intestinal inflammation in scid mice reconstituted with cd45rbhigh cd4+ t cells.the aim was to analyze the influence of intestinal microbiota on the development of intestinal inflammation. we used the model of chronic inflammation that develops spontaneously in the colon of conventional severe combined immunodeficiency (scid) mice restored with the cd45 rb(high) subset of cd4+t cells isolated from the spleen of normal balb/c mice.200717607724
investigation and treatment of a multiple limb birth.we report a baby born with three fully developed normally functioning legs. the third leg was attached to the baby's sacrum via an iliac bone at the site of a spina bifida. a primitive perineum was present. innervation of the limb was via a sacral hiatus with a myelomeningocele. a rudimentary peritoneal sac was found at operation containing a blind loop of bowel suspended by a mesentery. the additional leg was found to have no cortical representation using somatic evoked potentials although lumb ...20071892571
orthopaedic manifestations of sickle-cell disease.sickle-cell disease is a well-recognized clinical entity. the pathophysiology of this hemoglobinopathy has been described in detail by numerous investigators since the first case report appeared in 1910. orthopaedic manifestations of sickle-cell disease account for much of the morbidity associated with this disorder, including pain, osteonecrosis, arthritis, and sepsis. effective management of these bone and joint sequelae reflect accurate diagnosis, understanding of this disorder's pathophysiol ...20082238715
[questions and answers on urological care. management of a balloon catheter which cannot be removed smoothly]. 20082630750
assisting the patient to learn the "patient role". 20084381302
neurocristopathy syndrome: review of four cases.aberrant embryologic development of the neural crest may clinically result in one of several patterns of malformation. four examples of the neurocristopathy syndrome are described that collectively feature a very broad range of expression in these infants. because of the many expressions, the syndrome may not be recognized. we believe that greater familiarity with the disorder will aid in the discovery of unsuspected anomalies and provide clues about predisposing etiologies.20082359735
bacteroides (parabacteroides) distasonis splenic abscess in a sickle cell patient.splenic abscess is not an uncommon complication of patients with sickle-cell disease. here we describe an 18 year-old boy with sickle cell disease and left upper quadrant abdominal pain. computerized axial tomography revealed left sided free flowing pleural effusion and splenomegaly with liquefaction and possible gas formation. the splenic fluid grew an unusual organism known as bacteroides distasonis. the patient received antimicrobial therapy and underwent a splenectomy with full recovery. the ...200818176010
adherence and invasion of bacteroidales isolated from the human intestinal tract.members of the genera bacteroides and parabacteroides are important constituents of both human and animal intestinal microbiota, and are significant facultative pathogens. in this study, the ability of bacteroides spp. and parabacteroides distasonis isolated from both diarrhoeal and normal stools (n = 114) to adhere to and invade hep-2 cells was evaluated. the presence of putative virulence factors such as capsule and fimbriae was also investigated. adherence to hep-2 cells was observed in 75.4% ...200818828854
bioactivation of selenocysteine derivatives by beta-lyases present in common gastrointestinal bacterial species.studies in cell cultures and animal models have demonstrated cancer chemopreventive effects of certain selenium compounds. here we describe the screening of cysteine s-conjugate beta-lyase activity in bacterial species that are implicated in the bio-activation of sulfur- and selenocysteine derivatives. we screened a range of bacterial species commonly found in the human intestine for beta-lyase activity on se-p-methoxybenzylselenocysteine and the natural occurring s-methylcysteine and se-methyls ...200819326339
sistrunk revisited: a 10-year review of revision thyroglossal duct surgery at toronto's hospital for sick children.the definitive surgical management of thyroglossal duct cysts (tgdc) was elucidated by sistrunk in 1920. however, the procedure is often poorly performed. we reviewed the charts of 143 patients managed for tgdc at the hospital for sick children. toronto, between 1978-1988. these patients underwent 214 surgical procedures at hsc and elsewhere for cure. one hundred and five patients needed only one procedure. thirty-eight patients had recurrent disease. these needed 109 procedures for cure. inadeq ...20082593216
[thromboses of the subclavian vein and jugular vein and superior vena cava: sonographic diagnosis and control of treatment using tissue-type plasminogen activator therapy].in the suspected diagnosis "superior influx congestion" sonography performed with the 5 mhz linear scanner is suitable as a non-invasive method for the primary diagnosis of thrombotic occlusions of the vena subclavia and vena jugularis interna, and for follow-up monitoring under thrombolytic therapy. in successful lysis it is possible to verify a sonomorphological and doppler sonographic sequence of stages from the freshly formed, stratified stage via the high-echo to the low-echo thrombus with ...20082516362
an unusual cause of abdominal pain. 20082268772
[analysis of the nursing factors responsible for expanding the sphere of daily activities of patients with parkinson's disease]. 20082612141
[analysis of the nursing factors responsible for expanding the sphere of daily activities of patients with parkinson's disease]. 20082612141
cardiac hypersensitivity to 5-aminosalicylic acid. 20082566070
fifty hours for the poor? 20082612144
fifty hours for the poor? 20082612144
[shoulder joint arthroplasty--joint replacement]. 20082576713
a nationwide evaluation of the academic performance of male and female optometry students.the number of women entering optometry has risen steadily over the last two decades. the present study is a nationwide evaluation of the academic performance of male and female optometry students graduating in 1986. the data presented was obtained through the cooperation of 12 of the 15 u.s. schools and colleges of optometry. factors which were investigated include preoptometry grade point average (gpa), gpa at the end of the first two years of optometry school, gpa for the second two years of o ...20082394900
closed intramedullary nailing of diaphyseal fractures of the femur. problems related to anatomical variations of the greater trochanter.an examination of the radiographs of 100 normal femurs revealed an anatomical variation consisting of lateralization of the trochanteric mass in relation to the femoral diaphysis. this involves disalignment of the apex of the greater trochanter in relation to the medullary canal, with consequent complications during closed intramedullary nailing such as varus of the fracture segments, valgus of the nail, fracture of the medial cortex. since becoming aware of this anatomical variation and its pos ...20092737894
rural nursing: developing the theory base.many health care needs of rural dwellers cannot be adequately met through the use of existing nursing models alone but require unique approaches emphasizing the special needs of this population. the development of an integrated theory base for rural nursing is necessary. a retroductive approach, building upon both qualitative and quantitative research data, is being used to develop a theory of rural nursing. key concepts identified are: work beliefs and health beliefs; isolation and distance; se ...20092772454
[an epidemiologic study of the orodental status of 6-15-year-old children in the languedoc-roussillon region].an epidemiological study of a representative sample of 702 children, aged 6 to 15 years, was conducted in the languedoc-roussillon region composed of the departments of aude, gard, hérault, lozère and pyrénées orientales. the mean dmft at 12 years of 4.21 was always higher in girls (5.13) than in boys (3.46) of the same age. the dmft was lower in urban areas than in rural zones, but the difference was only statistically significant at 11 years of age. the pits and fissures were the most caries-a ...20092738052
the acid-etch retained rest for removable partial dentures.this article will describe the techniques for composite and metal rests. it will also discuss a clinical follow-up of 18 patients who have had metal or composite rests in place from three to 18 months.20092691082
a century of change in neurosurgery at johns hopkins: 1889-1989.neurosurgical admissions to the johns hopkins hospital during the first year of its existence, the first and last years of harvey cushing's practice, the last years of walter dandy's practice, the last years of earl walker's tenure, and the current neurosurgical case load are reviewed. sixteen patients with neurosurgical problems were admitted during the first year of the hospital's existence. in the first year of harvey cushing's staff appointment, 38 neurosurgical patients were admitted. infec ...20092681565
a neurophysiological study of children with reading, writing and spelling difficulties.recordings of early and late auditory evoked potentials and spectral analysis of scalp eegs of 14 eight- and nine-year-old boys with difficulties in reading, writing and spelling were compared with those of 18 matched controls. the recordings of the poor readers lacked the normal asymmetry of central conduction time and wave amplitude in the early (brainstem) auditory evoked potentials, and late (cortical) evoked potential components were reduced in amplitude. spontaneous eeg activity was signif ...20092806738
when to use posts. 20092698331
clinical studies of polymyalgia rheumatica. a proposal of diagnostic criteria.a differential diagnosis of polymyalgia rheumatica (pmr) and rheumatoid arthritis (ra) can be difficult in elderly patients, especially at the prodromal phase of ra. furthermore, it was felt that there might be a diagnostic criteria for pmr specific to the japanese. accordingly, we have retrospectively studied the pmr patients who had come to our hospital, for making a diagnostic criteria. we especially stressed the distinguishing of pmr from ra in elderly patients. the criteria proposed are as ...20092681899
the bioavailability of apigenin-7-glucoside is influenced by human intestinal microbiota in rats.we investigated the impact of human intestinal microbiota on bioavailability of the flavone apigenin-7-glucoside (a7g) by comparing germ-free and human microbiota-associated (hma) rats. first, the ability of the human intestinal microbiota to convert a7g was proven in vitro by incubating a7g with fecal suspensions. apigenin, naringenin, and 3-(4-hydroxyphenyl)propionic acid were formed as main metabolites. after application of a7g to germ-free rats, apigenin, luteolin, and their conjugates were ...200919403720
breast volume measurement of 598 women using biostereometric analysis.a study of the volumes of the right and left breasts of 598 subjects was undertaken using biostereometric analysis. this measurement uses close-range stereophotogrammetry to characterize the shape of the breast, and is noncontact, noninvasive, accurate, and rapid with respect to the subject involvement time. using chi-square tests, volumes and volumetric differences between breast pairs were compared with handedness, perception of breast size by each subject, age, and menstrual status. no signif ...20092729845
[long-term follow-up of vena cava filters: real-time sonography and the native x-ray image].in 16 patients with vena cava filters (12 günther filters, 4 kimray-greenfield filters) a follow-up study was performed by ultrasound and abdominal plain film. ultrasound allows to identify type and location of the filter as well as the entrapment of emboli. abdominal radiography is useful for exact evaluation of changes in filter position. combination of sonography and plain films is considered to be most suitable for follow-up studies of caval filters.20093035643
[causes of death and follow-up in life insurance. 5 years' study]. 20102873492
[current approaches to immunological diagnosis of autoimmune and immune complex diseases]. 20102974642
[surgery in the treatment of patients with small cell lung cancer].the data on surgical and combined treatment of 52 cases of small cell lung cancer are presented and the end results are evaluated. the findings on survival time make the case for surgery as a component of combined treatment of tumor. the best results were obtained in cases of stage i and ii tumor, absence of metastases into intrathoracic lymph nodes, tumor invasion limited to bronchopulmonary lymph nodes or the root of the lung, intermediate cell pattern of small cell lung cancer and surgery fol ...20102998088
lysyl oxidase in osteogenesis imperfecta and marfan's syndrome. 20102897892
correction of iatrogenic floating toe following resection of the base of the proximal phalanx.for many decades, resecting the base of the proximal phalanx was a popular method of correcting hammertoes. the long-term result of the procedure, however, is a flail, proximally retracted, "floating" toe. correction of this challenging surgical problem can be accomplished with a v-y advancement flap and specific releases.20102937523
communicating leveled clinical expectations to nursing students.nurse educators agree on the need for objectives in the classroom situation and frequently communicate them in a written form. written expectations for clinical, however, are rarely given to student nurses prior to their clinical experience. clinical expectations are understood by instructors, but communicating them to students result in mixed interpretations. the article describes the development of a written method of communicating clinical expectations to solve this problem. this method corre ...20103035139
cerebral perfusion pressure: a hemodynamic mechanism of mannitol and the postmannitol hemogram.sixteen patients each received infusions of 1 g of mannitol per kg over 5 to 10 minutes, and serial determinations of intracranial pressure (icp), systemic arterial blood pressure (sabp), central venous pressure, cerebral perfusion pressure (cpp), hematocrit, hemoglobin, serum na+, k+, osmolarity, and fluid balance were carried out for 4 hours. urine output was replaced volume for volume with 5% dextrose in 0.45% nacl solution. we tested the hypothesis that patients with high (greater than or eq ...20103116451
lessons learned from the anaerobe survey: historical perspective and review of the most recent data (2005-2007).the rationale and lessons learned through the evolution of the national survey for the susceptibility of bacteroides fragilis group from its initiation in 1981 through 2007 are reviewed here. the survey was conceived in 1980 to track emerging antimicrobial resistance in bacteroides species.201020067390
first report of parabacteroides goldsteinii bacteraemia in a patient with complicated intra-abdominal infection.using 16s rrna sequence analysis, we report the first isolation of parabacteroides goldsteinii as a monobacterial isolate from blood culture in a patient with abdominal sepsis. p. goldsteinii phenotypically resembles parabacteroides merdae and parabacteroides distasonis and may be misidentified by commonly used enzymatic systems, suggesting that it may be more frequently present in clinical specimens than previously appreciated but either misidentified or ignored.201020139022
detection of resistance genes and susceptibility patterns in bacteroides and parabacteroides strains.susceptibility to five antimicrobials was determined for bacteroides spp. (n = 52) and parabacteroides distasonis (n = 8). all isolates were susceptible to metronidazole. the resistance rates to ampicillin, cefoxitin, tetracycline and clindamycin were 98%, 9.6%, 65.3% and 19.2% of the bacteroides strains, respectively. the genes cepa, cfia, cfxa, tetq, ermf and nim were found in 69.2%, 17.3% 9.6%, 50%, 7.7% and 3.8% for these strains respectively. all p. distasonis strains were resistant to ampi ...201020159050
intestinal bacteroides species associated with coeliac disease.to characterise the predominant species of bacterial populations associated with duodenal biopsies of paediatric patients with active and treated coeliac disease.201020972239
epizootic rabbit enteropathy inoculum (tec4): antibiograms and antibiotic fractionation.epizootic rabbit enteropathy (ere) emerged and spread in europe within the last 13 years causing major economical loss. the aims of the study was to evaluate antibiograms of tec4, an inoculum composed of an extract of intestinal content of affected rabbits, and to test the potential of different antibiotic-based tec4 fractions to reproduce the disease. twenty nine different antibiotic discs were incubated for determining bacteria resistance. in a complementary study, nine tubes of liquid medium ...201020978841
oral administration of parabacteroides distasonis antigens attenuates experimental murine colitis through modulation of immunity and microbiota composition.commensal bacteria have been shown to modulate the host mucosal immune system. here, we report that oral treatment of balb/c mice with components from the commensal, parabacteroides distasonis, significantly reduces the severity of intestinal inflammation in murine models of acute and chronic colitis induced by dextran sulphate sodium (dss). the membranous fraction of p. distasonis (mpd) prevented dss-induced increases in several proinflammatory cytokines, increased mpd-specific serum antibodies ...201021087444
identification and characterization of a novel fumarase gene by metagenome expression cloning from marine microorganisms.fumarase catalyzes the reversible hydration of fumarate to l-malate and is a key enzyme in the tricarboxylic acid (tca) cycle and in amino acid metabolism. fumarase is also used for the industrial production of l-malate from the substrate fumarate. thermostable and high-activity fumarases from organisms that inhabit extreme environments may have great potential in industry, biotechnology, and basic research. the marine environment is highly complex and considered one of the main reservoirs of mi ...201021092234
resistant starches types 2 and 4 have differential effects on the composition of the fecal microbiota in human subjects.to systematically develop dietary strategies based on resistant starch (rs) that modulate the human gut microbiome, detailed in vivo studies that evaluate the effects of different forms of rs on the community structure and population dynamics of the gut microbiota are necessary. the aim of the present study was to gain a community wide perspective of the effects of rs types 2 (rs2) and 4 (rs4) on the fecal microbiota in human individuals.201021151493
effect of lumbar posture on lifting.twenty laborers assumed specific lumbar spine postures and lifted a 157 n crate to three different hand heights to determine if lumbar spine flexion moments or trunk muscle activity were affected by the lifting postures. lumbar flexion moments were lowest when the workers used the lordotic and straight back postures, while the average erector spinae muscle activity tended to be highest in the lordotic and straight back postures. the kypohotic posture regularly reduced the activity of the erector ...20102954217
use of reca protein to enrich for homologous genes in a genomic library.reca protein-coated probe has been utilized to enrich genomic digests for desired genes in order to facilitate cloning from genomic libraries. using a previously cloned hla-b27 gene as the reca-coated enrichment probe, we obtained a mean 108x increase in the ratio of specific to nonspecific plaques in lambda libraries screened for b27 variant alleles of estimated 99% homology to the probe. class i genes of lesser homology were less enriched: 6.7x for non-b27 genes of estimated greater than 95% h ...20102901713
brain tumor death risk in mental patients.several investigators have previously reported a low cancer mortality in mental patients. they hypothesized there were possibilities of actual exclusion between cancer and psychosis. on the other hand, some authors warned that the reported association between psychiatric illness and cancer death might vary, depending on the used statistical methods. to clarify this problem, we have examined the incidence of deaths caused by malignant neoplasm, particularly from brain tumor, in the chronic patien ...20102869114
possible association between breast cancer and malignant melanoma. 20102898922
possible association between breast cancer and malignant melanoma. 20102898922
[electron microscopy and histochemical studies of the origin and function of myoepithelioid cells in glomangioma and peripheral barrier arteries].epithelioid cells (myoepithelioid cells, glomus cells) are a structural element characteristic of hoyer-grosser shunts (glomus organs, arterio-venous epithelioid cell glomerula) and bailey's glomangioma (masson's angio-neuromyome-artériel, glomus tumors). their functional importance cannot be estimated without knowledge of their histogenetic origin. the myoepithelioid cells are derived from smooth muscle cells, which can be shown ultrastructurally, histochemically, or by light microscopy.20103020817
expression of t cell receptor gamma-chain in murine cytotoxic t cells: analysis of a highly transcribed nonrearranged gene cluster.the murine t cell receptor gamma-gene family is organized in 4 gene clusters which encode variable, joining and constant gene segments. because the function of the gamma-gene products is still unclear, there is great interest in functional rearrangements of these genes. we searched for new variable gamma-gene segments in the gamma 4-gene cluster. the mapping and sequencing of a supposed new variable segment of a functionally spliced transcript from a cdna library of the cytotoxic t cell clone 3f ...20102849571
[encapsulating peritonitis in periodic disease. apropos of a case studied by x-ray computed tomography].a case of encapsulating peritonitis complicating the course of familial mediterranean fever is reported. this encapsulating peritonitis was responsible for abdominal pain and had a "pseudocystic" appearance on ultrasonography and computed tomography. ultrasound guided aspiration produced a yellowish fluid rich in proteins and poor in cells. surgical operation revealed a congested appearance of the peritoneum associated with a richly fibrinous appearance of the external wall of the mass. the diff ...20102817728
influence of endoscopic papillotomy on the management of bile duct stones.in a 4 year period two hundred and seventy-nine patients were treated for gallstones in a single surgical unit. one hundred and thirteen were treated for stones in the bile duct, of them 49 patients were treated surgically and 64 endoscopically. twenty-three patients had papillotomy without previous cholecystectomy. there were two deaths, both after surgery following failure of endoscopic treatment. morbidity was greater in the surgically treated group. it is concluded that a large proportion of ...20103768645
report of the second public health service aids prevention and control conference. report of the workgroup on intravenous drug abuse. 20113147488
back pain. four. positive lifting. 20113156311
drgs and disease staging for reimbursing medicare patients.beginning october 1, 1983, medicare began reimbursing many hospitals on the basis of a set of fixed fees tied to diagnosis-related groups (drgs). using 1979-1981 maryland data for medicare patients, this paper compares the drg system with the disease staging patient classification system in terms of structure, explanation of resource consumption (length of stay) of hospital patients, and impact on reimbursement by type of hospital. the two systems are conceptually and empirically different in cl ...20113093783
[the role of the nurse and her education between the past and the future. analysis of realities in calabria]. 20113143127
food allergy. 20113202279
needle aspiration of nonperitonsillar head and neck abscesses. a six-year experience.twenty-five patients with various head and neck abscesses have been managed with needle aspiration as the initial surgical modality over a six-year period. twenty (83%) of 24 patients' abscesses resolved without formal surgical intervention. patient 25 in the series whose abscess, though initially resolving, reaccumulated within two weeks requiring an incision and drainage. recurrent cancer was then diagnosed. eight of ten large-volume abscesses (over 10 ml) were controlled with needle aspiratio ...20113166766
[hospital long term care]. 20113147353
antiimmunoglobulin-treated b cells respond to a b cell differentiation factor for igg1.we have determined whether b cells previously activated by anti-ig (anti-ig blasts) are responsive to lymphokines that induce isotype switching. culture of anti-ig blasts with a mixture of lymphokines, including bsf-1, resulted in marked secretion of igm and igg1, but not other igg isotypes. the igg1 response of anti-ig blasts to lymphokines was 13-fold greater than was observed with splenic b cells. b cell blasts induced by 8-mercaptoguanosine or dextran sulfate did not secrete high levels of a ...20113088197
differential suppression of testosterone and estradiol in hirsute women with the superactive gonadotropin-releasing hormone agonist leuprolide.to determine the dose of the gnrh agonist leuprolide necessary to maximally suppress ovarian testosterone secretion, 10 moderately to severely hirsute women (5 with idiopathic hirsutism and 5 with polycystic ovarian syndrome) were given gradually increasing leuprolide doses, starting with either 5 or 10 micrograms/kg.day. serum testosterone and estradiol, basal lh, and the lh response to gnrh were measured before and at the end of each treatment period, until maximal suppression of estradiol and ...20113138274
myasthenia gravis in childhood and adolescence. report on 209 patients and review of the literature.authors review the different theoretical and practical problems of childhood and adolescent myastenia gravis, including the heterogeneous group of congenital myasthenia and the big casuistics of the literature. there are reports on 113 cases with childhood myasthenia gravis and 96 cases of adolescent myasthenia. ratio of these forms ranged 10.76% in childhood myasthenia and 9.14% in adolescent age, resp., 19.9% of the whole patient material. a classification is given concerning juvenile myasthen ...20113077607
[experiences with the preventive use of aminophylline (euphylline) in apnea and periodic respiration in infants].sleep apnea and periodic breathing in infants are the result of a mild hypoxia and they are the requirement for sids. as a results of the modern medicine each 4.-5. death is the cause of sids during the first year of life. the positive effect of theophylline on apnea of prematurity is known since 1973. we have given theophylline (euphyllin) from 1979 till 1986 to 198 premature infants with a birth weight below 2000 g for apnea prophylaxis orally in a doses of 9 mg/kg body weight per day distribu ...20113043316
dietary resistant and butyrylated starches have different effects on the faecal bacterial flora of azoxymethane-treated rats.epidemiological studies have suggested that dietary fibre lowers the risk of colorectal cancer, which may be due to increased butyrate production from colonic fermentation of a type of fibre, resistant starch (rs). the present study investigated the effects of dietary rs and butyrylated rs on the faecal microbiota of rats treated with azoxymethane. a total of four groups of nine rats were fed diets containing either standard maize starch (low-amylose maize starch (lams), low rs), lams with 3 % t ...201121255474
application of quantitative real-time pcr for rapid identification of bacteroides fragilis group and related organisms in human wound samples.our goal was to establish a quantitative real-time pcr (qrt-pcr) method to detect bacteroides fragilis group and related organisms from clinical specimens. compared to conventional anaerobic culture, qrt-pcr can provide accurate and more rapid detection and identification of b.-áfragilis group and similar species. b.-áfragilis group and related organisms are the most frequently isolated anaerobic pathogens from clinical samples. however, culture and phenotypic identification is quite time-consum ...201121439390
design of species-specific oligonucleotide probes for the detection of bacteroides and parabacteroides by fluorescence in situ hybridization and their application to the analysis of mouse caecal bacteroides-parabacteroides microbiota.aims:  to develop species-specific monitoring techniques for rapid detection of bacteroides and parabacteroides inhabiting the mouse intestine by fluorescence in situ hybridization. methods and results:  the specificity of oligonucleotide probes was evaluated by fluorescence whole-cell hybridization. oligonucleotide probes specific for each species hybridized only with the target bacteria. using these probes, caecal bacteroides-parabacteroides microbiota of conventional mice and specific pathoge ...201121535330
antimicrobial resistance and prevalence of resistance genes in intestinal bacteroidales strains.this study examined the antimicrobial resistance profile and the prevalence of resistance genes in bacteroides spp. and parabacteroides distasonis strains isolated from children's intestinal microbiota.201121655744
metabolic labeling of fucosylated glycoproteins in bacteroidales species.members of the bacteroidales order are among the most abundant gram-negative bacteria of the human colonic microbiota. these species decorate their cell-surface glycoproteins with fucosylated glycans, which are believed to play important roles in host intestinal colonization. currently, there is no method for the enrichment of these glycoproteins for their identification. here, we describe a chemical approach directed toward labeling and detecting fucosylated glycoproteins from cultured bacteroi ...201121676614
Butyrate esterified to starch is released in the human gastrointestinal tract.Short-chain fatty acids (SCFAs) maintain human colonic function and may help prevent colonic disease. A study with ileostomists showed that starches acylated with specific SCFAs largely survive passage through the small intestine, but the percentage released in the colon has not been established.201121940597
prevalence of antimicrobial resistance among clinical isolates of bacteroides fragilis group in canada in 2010-2011: canward surveillance study.clinical isolates of bacteroides fragilis group (n = 387) were collected from patients attending nine canadian hospitals in 2010-2011 and tested for susceptibility to 10 antimicrobial agents using the clinical and laboratory standards institute (clsi) broth microdilution method. b. fragilis (59.9%), bacteroides ovatus (16.3%), and bacteroides thetaiotamicron (12.7%) accounted for ∼90% of isolates collected. overall rates of percent susceptibility were: 99.7%, metronidazole; 99.5%, piperacillin-t ...201122203594
[primary transport of acute patients. optimal use of rescue resources exemplified by the canton of graubünden]. 20113927607
lipid and lipoprotein effects on endothelial eicosanoid formation.an important function of endothelium is to release pgi2, a prostaglandin produced from arachidonic acid that prevents platelet aggregation and causes arterial relaxation. small amounts of other eicosanoids also are produced, but their role in endothelial function has not been elucidated. much of the arachidonic acid present in the endothelium is obtained preformed from the plasma, either as ffa or from lipoproteins. arachidonic acid is efficiently incorporated into endothelial lipids even when o ...20113061005
metastatic adenocarcinoma of the carpus: a case report.a patient is described who presented with a painful swelling of the dorsum of the hand which arose from the hamate and proved histologically to be a secondary from a previously resected gastric adenocarcinoma.20113171299
the 12-hour control of intraocular pressure on carteolol 2% twice daily.in a 15-week double-blind, placebo-controlled, crossover study both carteolol 2% and timolol 0.5% produced a significant reduction in intraocular pressure. twelve hours after administration the reduction in pressure on carteolol was significantly less than that obtained on timolol.20113067744
environmental stress, reactivity and ischaemic heart disease.this article provides an overview of work in two areas of biobehavioural research: the effects of environmental stress and the role of psychophysiologic reactivity in the development of ischaemic heart disease. attention is given first to evidence that low socio-economic status, low social support, and occupational settings characterized by high demands and low levels of control over the job are associated with increased coronary risk. also discussed is a promising animal primate model of social ...20113129010
[results of examination of a population from a region with a high incidence of esophageal cancer: precancerous lesions of the esophageal mucosa].esophagoscopic evidence of chronic esophagitis of varying degree was found in 60% of the total number of 1348 examined males living in a region with high incidence of esophageal cancer. the high incidence of chronic esophagitis corroborates the hypothesis that this disease is a precursor of esophageal cancer.20112943079
[results of examination of a population from a region with a high incidence of esophageal cancer: precancerous lesions of the esophageal mucosa].esophagoscopic evidence of chronic esophagitis of varying degree was found in 60% of the total number of 1348 examined males living in a region with high incidence of esophageal cancer. the high incidence of chronic esophagitis corroborates the hypothesis that this disease is a precursor of esophageal cancer.20112943079
obesity in children. 20113257638
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