Publications

the flexibility of 'box-loop', 'cross loop' and 'vertical loop' systems in the vertical plane.		1980	273442
[chronic digestive loss of plasma proteins evaluated with 51cr-albumin in billroth i and ii gastrectomized patients].	the digestive clearance of albumin 51cr was determined in patients submitted to gastrectomy due to peptic ulcer, 6 to 18 months after surgery. patients were of both sexes, with ages varying from 24 to 70 years. reconstruction after resection was according to billroth i and ii techniques (groups b and c). the group of control (group a) presented no digestive illness. each group was represented by 10 individuals. the mean value and respective standard derivation for each group was respectively: gr ...	1980	550789
pathogenesis of myocarditis in rocky mountain spotted fever.	because myocarditis has been noted frequently as present at necropsy in fatal rocky mountain spotted fever (rmsf) and ecg alterations may also accompany the disease, we studied the pathogenesis of myocarditis in rmsf. increased heart weight in eight of nine cases and increased interstitial volume in six of nine cases suggest that myocardial edema is present. specific immunofluorescent demonstration of rickettsia rickettsii in myocardial capillaries, venules, and arterioles in eight of nine cases ...	1980	6767463
properties of selected rickettsiae of the spotted fever group.	eight strains of spotted fever group rickettsiae were studied to gain insight into the extent of variation of their properties. two standard strains of rickettsia rickettsii and one strain of rickettsia conorii were included among the eight for comparison. the molar percentage of guanine plus cytosine for each strain did not differ significantly from that for r. rickettsii, 32.6 +/- 0.7%. two strains caused extended fever in guinea pigs, one strain caused fever of short duration, and the other s ...	1980	6769801
development and characterization of high-titered, group-specific fluorescent-antibody reagents for direct identification of rickettsiae in clinical specimens.	rabbits were inoculated with purified antigen preparations of coxiella burnetii and representative species of the spotted fever and typhus groups of rickettsiae. their antibody responses were monitored by complement fixation tests; high-titered antisera were fractionated with ammonium sulfate and then labeled with fluorescein isothiocyanate by the dialysis method. the conjugates had homologous 3+ staining titers of 1:256 to 1:2,048 and did not exhibit nonspecific staining. the rickettsia rickett ...	1980	6769955
measles in patients suspected of having rocky mountain spotted fever.	diagnostic titer rises of measles antibody were observed in six of 46 paired sera from persons originally suspected of having rocky mountain spotted fever and found to be serologically negative for this disease. atypical or typical measles is now occurring relatively more frequently in adolescents and young adults and should be considered in the differential diagnosis for patients in these age groups who have undiagnosed febrile rash illnesses.	1980	6771427
rickettsial infection of the pulmonary microcirculation: the basis for interstitial pneumonitis in rocky mountain spotted fever.	pulmonary tissue from 10 patients with fatal rocky mountain spotted fever was examined by brightfield microscopy for histopathologic lesions and by immunofluorescence for rickettsia rickettsii. the distribution of rickettsiae and the vasculitis of the pulmonary microcirculation coincided. the lungs demonstrated the consequent interstitial pneumonia--alveolar septal congestion and interstitial edema; alveolar edema, fibrin, macrophages, and hemorrhage; and interlobular septal edema. the effects o ...	1980	6772542
spotted fever group rickettsiae in dermacentor variabilis from cape cod, massachusetts.	spotted fever group (sfg) rickettsiae were identified by indirect immunofluorescent antibody tests in 1.1% of 6,956 specimens of dermacentor variabilis collected on cape cod, massachusetts. forty-one of 45 rickettsial isolates were serotyped as rickettsia montana, one as rickettsia rickettsii, and three were unidentified. studies of canines and humans with clinical evidence of spotted fever, and of healthy dogs with antibody to sfg rickettsiae, indicated that exposure of the canine population to ...	1980	6773430
the rickettsial plaque. evidence for direct cytopathic effect of rickettsia rickettsii.	monolayers of primary chick embryo cells were infected with rickettsia rickettsii in a plaque assay system with 0.5 per cent agarose overlay. plaques appeared 5 days after inoculation and were examined on day 6 by supravital staining, immunofluorescence for r. rickettsii, and electron microscopy. in all studies maintenance of the topographic integrity of the monolayer and the plaque allowed assessment of temporospatial relationships of rickettsial infection and cytopathologic changes. plaques co ...	1980	6777602
laboratory diagnosis of rocky mountain spotted fever.	to examine the use of the laboratory in the diagnosis of rocky mountain spotted fever and to determine the specificity and sensitivity of the weil-felix test, hemagglutination, complement fixation, and skin biopsy immunofluorescence in the hospital, we reviewed our experience during th year 1978. sera were submitted from 142 patients and skin biopsies from 16 patients suspected of having rmsf. sensitivity rates of methods in the acute phase were skin biopsy, 70%; proteus ox-19 agglutination, 65% ...	1980	6777873
serological evidence of infection by spotted fever group rickettsiae in karnataka state.		1980	6780455
detection of rocky mountain spotted fever antibodies by a latex agglutination test.	a latex test for immunodiagnosis of rocky mountain spotted fever, using erythrocyte-sensitizing substance from rickettsia rickettsii adsorbed to latex particles, has been developed. the test was evaluated with a total of 123 single and 118 paired human sera submitted for rocky mount spotted fever testing. this test is simple, sensitive, and specific. its efficiency, relative to the reference microimmunofluorescence test, was 95.1% for single sera and approached 100% for paired sera.	1980	6785303
in vitro studies of rickettsia-host cell interactions: ultrastructural study of rickettsia prowazekii-infected chicken embryo fibroblasts.	secondary chicken embryo fibroblasts infected in suspension with the breinl strain of rickettsia prowazekii and grown in monolayer culture were examined by both transmission and scanning electron microscopy at specific intervals after infection to study the effects of prolonged intracellular growth on the fine structure of the host cell and the rickettsiae. cytopathological changes in the infected host cells were not apparent until late in the intracellular growth cycle when the cells began to r ...	1980	6783534
rocky mountain spotted fever in clermont county, ohio. ii. distribution of population and infected ticks in an endemic area.	the epidemiology of rocky mountain spotted fever (rmsf) in relation to the distribution of the human population and infected ticks has not been defined. a study was undertaken in clermont county, ohio, to describe the distribution of population in a highly endemic focus of rmsf and to determine the prevalence of ticks infected with rickettsiae of the spotted fever group. this area was compared to another area of similar size in the same county where the incidence of rmsf was much lower. the popu ...	1980	7352458
the role of cottontail rabbits (sylvilagus spp.) in the ecology of rickettsia rickettsii in the united states.	rocky mountain (sylvilagus nuttallii) and eastern (s. floridanus) cottontails were examined for their susceptibility to virulent and avirulent strains of the spotted fever agent, rickettsia rickettsii. both species of rabbits responded to inoculation of yolk sac suspensions containing 500 egg ld50 of either virulent or avirulent rickettsiae, with rickettsemias detectable as early as 3 days after inoculation and lasting up to 7 days. when fed upon by infected ticks, only one of three rocky mounta ...	1980	7406116
serological cross-reaction and cross-protection in guinea pigs infected with rickettsia rickettsii and rickettsia montana.	antisera produced in guinea pigs inoculated with rickettsia rickettsii or rickettsia montana were cross-reactive but sufficiently specific to identify the primary infecting agent. guinea pigs immunized with r. montana were protected from fatal infection with r. rickettsii, although a few (25%) developed mild fever of short duration.	1980	6893191
the occurrence of eschars in rocky mountain spotted fever.	two patients with fatal, laboratory-confirmed rocky mountain spotted fever (rmsf) were noted early in their course to have a skin lesion characteristic of a rickettsial eschar. postmortem immunofluorescent and histopathologic studies demonstrated that the lesions were sites of extensive contiguous infection by rickettsia rickettsii with associated injury to numerous local blood vessels. rickettsial vasculitis and occlusive luminal thrombosis were associated with dermal and epidermal coagulative ...	1981	7240465
differential requirements for enriched atmospheric carbon dioxide content for intracellular growth in cell culture among selected members of the genus rickettsia.	in an in vitro chicken embryo cell culture system, strains of rickettsia prowazekii, r. mooseri, and r. rickettsii, but not of r. tsutsugamushi, required an atmosphere enriched in co2 for intracellular growth.	1981	6785241
epidemic typhus in the united states associated with flying squirrels.	between july 1977 and january 1980, seven cases of sporadic, nonepidemic "epidemic" typhus (rickettsia prowazekii) were discovered in virginia, west virginia, and north carolina. the reservoir seemed to be the southern flying squirrel (glaucomys volans), an animal indigenous to the eastern united states; however, the vector or mode of acquisition was not evident. diagnosis was established principally through complement fixation, indirect immunofluorescence, and toxin neutralization tests. patien ...	1981	6785459
fatty acid composition of rickettsiae.	the fatty acid compositions of selected strains of rickettsiae were studied by gas-liquid chromatography. the profiles of all the rickettsiae except coxiella burnetii were qualitatively similar. the fatty acid composition of c. burnetii was similar to that of certain legionella species.	1981	6787071
endemicity of spotted fever group rickettsiae in connecticut.	to compare rickettsial infectivity and seropositivity rates against spotted fever group (sfg) rickettsiae, ticks and wild mammals were collected from three areas where rickettsia rickettsii was thought to be enzootic in connecticut during 1978-1979, and from four additional sites (with no reported human cases) between 1976 and 1979. of the 1,001 dermacentor variabilis examined by the hemolymph test, 59 (5.9%) contained rickettsia-like organisms; direct immunofluorescence tests verified the prese ...	1981	6789690
serotypes of tick-borne spotted fever group rickettsiae from western california.	a rickettsial survey of ixodid ticks known to bite man was conducted in 1979 in four coastal counties of california to obtain isolates from tick species that might be involved in the transmission of spotted fever-like illnesses, and to examine serologic characteristics of the rickettsiae relative to defined members of the spotted fever group (sfg). one hundred and seventy (19.4%) of 877 ticks comprising three species were shown by hemolymph test to harbor rickettsia-like organisms. a total of 85 ...	1981	6789691
rocky mountain spotted fever in childhood.		1981	6798547
the liver in rocky mountain spotted fever.	hepatic tissues from nine patients who had fatal rocky mountain spotted fever were examined in sequential sections by brightfield and immunofluorescence microscopy for histologic lesions and for coincidence of these lesions with the distribution of rickettsia rickettsii. the basic hepatic lesion in rocky mountain spotted fever is an inflammation of the portal triad in which large mononuclear cells and neutrophils predominate. rickettsia were demonstrated in lesions of the portal triads in eight ...	1981	6781327
serotypes of spotted fever group rickettsiae isolated from dermacentor andersoni (stiles) ticks in western montana.	adult dermacentor andersoni ticks were collected by flagging vegetation in 18 canyons bordering the bitterroot valley, montana, an area where nearly 400 cases of rocky mountain spotted fever (rmsf) have occurred since 1900. three hundred and nine (8.3%) of the 3,705 ticks collected contained hemocyte-associated rickettsia-like organisms of three morphologic types, coccobacillary, fine bacillary, and coarse bacillary (long forms). only the coccobacillary and fine bacillary organisms stained with ...	1981	6782899
rocky mountain spotted fever. serological evidence of previous subclinical infection in children.	serological tests for rocky mountain spotted fever were performed on single serum specimens from sixth graders from forsyth county, north carolina, an area highly endemic for this disease. five of 508 *.098%) sera were positive (titer greater than or equal to 1:64) using the indirect fluorescent antibody method. elevated proteus agglutination antibody titers (greater than 1:160) to ox-19, but not ot ox-2, were common (19.6%) in these children. no correlation of those with positive ox-19 titers c ...	1982	6798859
in vitro sensitivity of rickettsia rickettsii to doxycycline.		1982	6802907
antibodies to spotted fever-group rickettsiae in dogs and prevalence of infected ticks in southern connecticut.	blood samples and ticks were obtained from dogs to assess canine exposure to spotted fever-group (sfg) rickettsiae during 1978-1980 in southern connecticut. of the 1,576 dog sera screened by microimmunofluorescence. 174 (11.0%) contained specific antibodies at titers greater than or equal to 1:64 against rickettsia montana (n = 34), r rickettsii (n = 31), r rhipicephali (n = 19), or the unclassified 369-c rickettsia (n = 90). end points greater than or equal to 1:8,192 to r rickettsii and to r r ...	1982	6803626
rocky mountain spotless fever: a dilemma for the clinician.	failure of the characteristic rash to develop during the course of illness in rocky mountain spotted fever may lead to the delay or failure of diagnosis and may result in fetal encephalopathic illness with disseminated vascular injury. four patients are described herein in whom a rash failed to develop and the diagnosis was initially incorrect. each patient was seen at least once before hospital admission by a physician and given ineffective antibiotic therapy, resulting in fatal complications. ...	1982	6807231
rocky mountain spotted fever in areas of high and low prevalence: survey for canine antibodies to spotted fever rickettsiae.	antibodies to rickettsia rickettsii were detected by indirect immunofluorescence in sera from 149 of 467 dogs (32%) examined from 4 military installations located in kentucky, north carolina, pennsylvania, and virginia. the prevalence at individual installations ranged from 4.3% at fort knox, ky, to 63.4% at fort bragg, nc. most of the seropositive dogs were in the working and sporting groups of dogs. the difference in antibody prevalence between sexes was not significant. serologic responses we ...	1982	6808878
human endothelial cell culture plaques induced by rickettsia rickettsii.	primary cultures of human umbilical vein endothelial cells were inoculated with plaque-purified rickettsia rickettsii. after adsorption of rickettsiae, monolayers were overlaid with medium containing 0.5% agarose. small plaques appeared on day 4 postinoculation, and distinct 1- to 2-mm plaques were observed on day 5. plaquing efficiency was less than that of primary chicken embryo cells in the same medium. human endothelial cell monolayers were susceptible to infection by r. rickettsii and under ...	1982	6809631
penicillin-induced unstable intracellular formation of spheroplasts by rickettsiae.	penicillin g (greater than or equal to 20 micrograms/ml) is rapidly rickettsiacidal for intracellular rickettsia prowazekii. light and electron microscopic examinations revealed that penicillin g in culture medium induced a predictable transformation into typical enlarging spheroplasts deficient in the internal, putative peptidoglycan layer of the outer membrane. under certain conditions, spheroplasts ruptured to discharge contents into host cell cytoplasm and to leave empty shells of defective ...	1982	6809842
reactivation of rickettsia rickettsii in dermacentor andersoni ticks: an ultrastructural analysis.	virulent rickettsia in dermacentor andersoni lose their pathogenicity and virulence for guinea pigs when subjected to physiological stresses, such as starvation (overwintering), of its tick vector. however, incubation of infected ticks at an elevated temperature (37 degrees c) for 24 to 48 h or feeding for a time (usually greater than 10 h) induces r. rickettsii to revert to a virulent state, a phenomenon defined as "reactivation." electron microscopy reveals that the microcapsular and slime lay ...	1982	6811439
establishment of cell cultures persistently infected with spotted fever group rickettsiae.	a cell line was established from the tunica vaginalis of meadow voles, microtus pennsylvanicus, which could be persistently infected with the spotted fever group rickettsiae, rickettsia rickettsii, r. rhipicephali, and r. slovaca. as determined by light and electron microscopy, all cells in the cultures became infected and remained so even after 20 serial passages over a period of months. the rickettsiae-infected vole cell line is an excellent experimental model to study the noncytolytic host-ce ...	1982	6820331
[dr. ignacy kwarta].		1982	347527
[etiology and pathogenesis of neurological symptoms in low bite].	50 patients were subjected to complex examination. in bite lowering the head of the condyle is displaced backwards and upwards into the depth of the glenoid fossa. the pressure of the displaced mandibular joints upon the arterial cord and the tympanic vein as well as upon the venous network around the mandibular joint capsule may produce disturbed blood supply to the mucosa of the middle ear and of the auditory tube, and finally, obstruction of the auditory tube. disturbances in the blood supply ...	1983	270856
carcinoma of the lung presenting as cushing's syndrome.	a case of bronchial carcinoma of the lung presenting as cushing's syndrome is described. the clinical features and laboratory investigations leading to the diagnosis are outlined. the autopsy findings, histology and the ultrastructure of the tumour are illustrated and the literature is reviewed.	1983	264633
[unusual treatment of a large interdental gap].		1983	270799
chemomechanical removal of pulpal and dentinal debris with sodium hypochlorite and hydrogen peroxide vs normal saline solution.		1983	264933
problem solving in orthodontic and orthognathic surgery.		1983	264847
[the occurrence of rickettsiosis of the rickettsia rickettsii group].		1983	6675165
rocky mountain spotted fever in an urban canine population.	a survey for the prevalence of rocky mountain spotted fever antibodies in dogs from a well-defined endemic area in columbus, ohio (franklin county), was conducted during the summer of 1981. seventy-three blood samples from dogs in this area were tested by microimmunofluorescence for antibodies to rickettsia rickettsii and other rickettsial antigens. thirty-three (45.2%) of these samples were positive for r rickettsii, with titers ranging from 1:8 to 1:2,048. for comparison, 137 blood samples fro ...	1983	6418697
rocky mountain spotted fever in dogs associated with human patients in ohio.		1983	6418831
biological properties of rabbit antibodies to a surface antigen of rickettsia rickettsii.	because of the potential significance of external components of the obligate intracellular parasite rickettsia rickettsii in host-parasite interactions, we have begun the first phase of a study to isolate and characterize surface antigens of this organism. an antiserum to a rickettsial surface component was obtained from rabbits inoculated with immune precipitates prepared by crossed immunoelectrophoresis of triton x-100 extracts of r. rickettsii strain r. this antiserum (i) protected guinea pig ...	1983	6403467
role of phospholipase-associated penetration mechanism in cell injury by rickettsia rickettsii.	the cholesterol receptor-binding drugs amphotericin b and digitonin reduced rickettsial plaque formation, presumably by blocking rickettsial attachment, even when added to the plaque model on day 4 post-inoculation when infected foci were well established. the phospholipase inhibitor phentermine reduced plaque formation, presumably by inhibiting the phospholipase-associated entry step of penetration.	1983	6404837
absorption of rickettsia rickettsii antibodies by rickettsia rickettsii antigens in four diagnostic tests.	immunoabsorption profiles were determined for rickettsia rickettsii antigens used in four confirmatory tests for detection of antibodies to rocky mountain spotted fever. a human serum reactive in the four tests was absorbed with each test antigen and then reexamined by all four tests. the results indicated that the whole organism and complement-fixation antigen absorbed the whole array of antibodies to r. rickettsii, whereas erythrocyte-sensitizing substance coated on latex or erythrocytes did n ...	1983	6404927
latex-rickettsia rickettsii test reactivity in seropositive patients.	in correlating results obtained from a new latex-rickettsia rickettsii test with results obtained from a reference microimmunofluorescence test for rocky mountain spotted fever, we found that for seropositive patients each microimmunofluorescence titer (up to 4,096) was usually double the median titer obtained from the latex-r. rickettsii test. the pattern of immunoserological response indicated that latex-r. rickettsii is reactive with sera containing either anti-r. rickettsii immunoglobulin m ...	1983	6404929
rickettsiae-infected ticks (acari: ixodidae) and seropositive mammals at a focus for rocky mountain spotted fever in connecticut, usa.		1983	6405035
summertime and ticks.		1983	6405617
correlation of rickettsial titers, circulating endotoxin, and clinical features in rocky mountain spotted fever.	blood rickettsial titers, skin biopsy results, and circulating endotoxin measurements were correlated with the clinical course of disease in patients with rocky mountain spotted fever (rmsf). nine of 11 patients with documented rmsf had rickettsia rickettsii isolated from plasma samples. of the eight patients in whom rickettsial titers were measured, seven had 10(0.7) to 10(1.2) median tissue culture infective doses (tcid50) per milliliter; all seven had mild to moderately severe disease. one pa ...	1983	6407418
[survey of natural infection of ticks with rickettsia of the spotted fever group in the northwestern section of xinjiang province].		1983	6411346
detection of fibrils associated with rickettsia rickettsii.	the ultrastructural appearance of the "halozone" formed at the interface between the spotted fever agent rickettsia rickettsii and the cytoplasm of persistently infected cultured vole cells (microtus pennsylvanicus) was studied by transmission electron microscopy. in sections of epoxy-embedded specimens stained with uranyl acetate and lead citrate, the halozone appeared clear and devoid of ultrastructural features. however, when unembedded preparations of whole infected cells were examined at 1, ...	1983	6411620
coating of polymeric surfaces for immunoassay by a forced adsorption technique.	a forced adsorption technique for coating polymeric surfaces is described. this technique is simple and allows us to use (i) relatively impure ligands to be coated on latex surface and (ii) the minimum amount of ligands for preparation of large-volume lots of latex reagent. the serologic results are highly reproducible from lot to lot of the reagent, in contrast to the variability found with passively adsorbed reagents. use of this technique could be extended to coat flat surfaces for solid-phas ...	1983	6411771
rocky mountain spotted fever vaccine in an animal model.	formalin-killed, purified rickettsia rickettsii vaccine was evaluated in a guinea pig model of r. rickettsii infection. vaccinated guinea pigs were partially protected by the vaccine when challenged with virulent, viable rickettsiae. greater protection was observed when higher doses of vaccine were given and when frequent booster injections were administered. stimulation of cell-mediated immunity to the vaccine antigens was variable and also appeared to be achieved more reproducibly with booster ...	1983	6413529
detection of rickettsia rickettsii antibodies in human sera by crossed immunoelectrophoresis.	to identify rickettsia rickettsii antigens of immunological importance, we examined sera from patients with serologically confirmed cases of rocky mountain spotted fever by crossed immunoelectrophoresis for antibodies to antigens extracted from the r strain of r. rickettsii with the detergent triton x-100. sixteen antigens were identified in the detergent extract by crossed immunoelectrophoresis with a hyperimmune rabbit serum raised against whole rickettsiae. when the rabbit antiserum was place ...	1983	6415093
evaluation of latex-rickettsia rickettsii test for rocky mountain spotted fever in 11 laboratories.	a latex-rickettsia rickettsii test for detection of antibodies to rocky mountain spotted fever (rmsf) was evaluated during the 1980 rmsf season in 11 laboratories in nine states where the disease is endemic. in a double-blind study, all sera submitted to each laboratory for rmsf testing were also examined by the latex-r. rickettsii test. a portion of each specimen was then sent to the new york state laboratory for testing by latex-r. rickettsii and by the reference microimmunofluorescence test. ...	1983	6415103
serodiagnosis of rocky mountain spotted fever: comparison of igm and igg enzyme-linked immunosorbent assays and indirect fluorescent antibody test.	to characterize igm and igg antibody responses in rocky mountain spotted fever (rmsf), a microtiter enzyme-linked immunosorbent assay (elisa) using density gradient-purified rickettsia rickettsii as antigen was developed. sera of vaccinated individuals and patients with rmsf were tested by elisa and by indirect fluorescent antibody (ifa) tests. diagnostic agreement between elisa and the ifa test was 76% and 52% for igg and igm antibody, respectively. diagnostic agreement between the elisa for ig ...	1983	6415180
reactogenicity, immunogenicity, and efficacy of a chick embryo cell-derived vaccine for rocky mountain spotted fever.	a new formalin-inactivated vaccine prepared by sucrose density gradient centrifugation of tissue culture-grown rickettsia rickettsii was evaluated for safety and immunogenicity in a placebo-controlled, double-blind study. most of the 52 seronegative adult vaccinees, 88% after the first and 66% after the second dose, experienced brief, mild (mostly local) reactions, but only 50% exhibited a systemic immune response to vaccination. six unvaccinated volunteers (controls) and 16 of these vaccinees w ...	1983	6415182
susceptibility of inbred mice to rickettsiae of the spotted fever group.	a mouse strain susceptible to lethal infection with rickettsia conorii was required for testing vaccine efficacy and for studying the immunology and pathogenesis of infection. among 20 strains of inbred mice inoculated intraperitoneally with the malish strain of r. conorii, the c3h/hej mouse strain was the most susceptible, with a 50% lethal dose of approximately 10 pfu. infection of all mouse strains resulted in a measurable antibody response; the highest titers correlated with the greatest deg ...	1984	6418657
infection of human vascular endothelial cells by rickettsia rickettsii.	rocky mountain spotted fever is caused by rickettsia rickettsii, an obligate intracellular bacterial parasite. the organism primarily attacks endothelial cells and occasionally attacks smooth-muscle cells of small blood vessels. an effective means of examining host-parasite interaction in rocky mountain spotted fever would be to use an in vitro model system with a host-cell type that is similar in structure and function to the putative target cell in human infections. because human umbilical-vei ...	1984	6421942
rocky mountain spotted fever vaccine: a regional need.	because the killed rickettsia vaccines have failed to provide protection against rocky mountain spotted fever, we approached the problem of protective immunity to rickettsia rickettsii with a closely related, live, less pathogenic spotted fever rickettsia. guinea pigs vaccinated with r conorii produced antibodies to spotted fever group rickettsiae and did not have fever or other signs of illness after challenge with r rickettsii. a vaccine against rocky mountain spotted fever would be useful in ...	1984	6424239
biochemical and immunochemical analysis of rickettsia rickettsii strains of various degrees of virulence.	six strains of rickettsia rickettsii from montana and north carolina were examined in an effort to identify rickettsial constituents associated with virulence. fever responses, scrotal reactions, and mortalities of male guinea pigs inoculated intraperitoneally with 1,000 pfu of rickettsial strains revealed that the two montana patient strains ( sheila smith and norgaard ) and one montana strain ( sawtooth female 2) from the wood tick, dermacentor andersoni, could be placed in the group of highes ...	1984	6427110
effect of synthetic protease inhibitors of the amidine type on cell injury by rickettsia rickettsii.	to evaluate the importance of proteolytic activity in the pathogenesis of cell injury by rickettsia rickettsii, a series of four aromatic amidine inhibitors of trypsin-like proteases were introduced into the plaque model. the compounds were shown to be active toward plaque reduction with their order of effectiveness parallel to their antitrypsin activity. one of the compounds, bis(5-amidino-2-benzimidazolyl)-methane, at a concentration of 10(-5) m demonstrated complete inhibition of plaque forma ...	1984	6428311
epidemiology of rocky mountain spotted fever in ohio, 1981: serologic evaluation of canines and rickettsial isolation from ticks associated with human case exposure sites.	a survey for the prevalence of rocky mountain spotted fever (rmsf) antibodies in dogs associated with confirmed human cases in ohio was conducted during 1981. twelve of 14 confirmed cases (85%) had a history of dog association prior to onset of rmsf. a total of 29 dogs were included in the study, with 16 dogs providing serum samples for antibody testing and the remainder providing tick samples. serum samples tested by indirect microimmunofluorescence techniques revealed 12/16 dogs (75%) to be se ...	1984	6435461
rocky mountain spotted fever. gastrointestinal and pancreatic lesions and rickettsial infection.	recent clinical studies have shown a high incidence of nausea, vomiting, diarrhea, and abdominal pain in rocky mountain spotted fever (rmsf), and case reports have documented rickettsial infection and vascular injury in the small intestine, appendix, and gallbladder. to determine the incidence and distribution of rickettsia rickettsii and rickettsial lesions that might be the basis for these clinical manifestations of rmsf, tissues that were available from the stomach, small intestine, colon, an ...	1984	6439172
susceptibility and reservoir potential of the dog to spotted fever-group rickettsiae.	this study was initiated to determine the degree of susceptibility of dogs to virulent and nonvirulent spotted fever-group rickettsiae and to evaluate dogs as sources of infection for ticks. dogs were exposed either by inoculation (syringe) or by infective tick bite to the following rickettsial serotypes: (1) rickettsia rickettsii (wachsmuth and sawtooth female 2 strains), (2) r montana (m/5-6 b strain), and (3) r rhipicephali (3-7-female 6 strain). results indicated that dogs inoculated with 1, ...	1984	6548617
injury restricted to cells infected with spotted fever group rickettsiae in parabiotic chambers.	one chamber of paired parabiotic chambers separated by 0.2 micron poresized membrane filters which prevented passage of rickettsiae were infected with either rickettsia rickettsii or r. conorii. infected vero cell monolayers underwent necrosis. uninfected monolayers in adjoining chambers which shared the same extracellular milieu including soluble rickettsial products did not undergo necrosis.	1984	6152112
production and characterization of monoclonal antibodies to rickettsia rickettsii.	five mouse ascitic fluids (mafs) containing monoclonal antibody to rickettsia rickettsii were produced from three original fusions by murine hybridoma technology. the five mafs were fractionated and purified; each contained monoclonal antibody of the immunoglobulin g2a subclass. each monoclonal antibody-containing maf was titrated by indirect immunofluorescence against three r. rickettsii isolates from humans and four other spotted fever group rickettsiae. each maf was also titrated in the compl ...	1984	6209219
rickettsia rickettsii-induced cellular injury of human vascular endothelium in vitro.	the endothelial cell is the putative primary target cell in humans infected with rickettsia rickettsii, the etiological agent of rocky mountain spotted fever. although the clinical manifestations of infection by this organism are well documented, the mechanism of injury to the endothelial cell is not understood. the ability to culture human endothelial cells in vitro provides a unique system with which to study this host-parasite interaction directly. human vascular endothelial cells derived fro ...	1984	6724689
antibodies to rickettsia rickettsii in peromyscus leucopus from a focus of rocky mountain spotted fever in connecticut.	during 1980-1982, white-footed mice (peromyscus leucopus) were captured in newtown, connecticut, an area where rickettsia rickettsii, the etiologic agent of rocky mountain spotted fever, is thought to be enzootic. an indirect microimmunofluorescence test identified specific antibodies to this organism in 16 of 237 (7%) sera: titration end points for 14 samples were relatively high (1:128-1:2048). antibodies were detected in mice during 1980 and 1981 with monthly prevalences varying from 8 to 22% ...	1984	6744122
[liver injury during combined chemotherapy of acute leukemias].	the authors observed 70 patients with acute leukemia treated by polychemotherapy. in 2 patients the signs of severe liver damage were found. the occurrence, course and results of laboratory tests suggested purinethol predominantly as the cause of development of these alterations. the complication did not occur frequently in our patients. the changes regressed in a satisfactory way after the withdrawal of the hepatotoxic cytostatic from the therapy.	1984	263624
[use of the direct antiglobulin test in capillary tubes in the diagnosis of abo hemolytic disease of the newborn. critical review after 7 years experience].		1984	263617
[is somnambulism related to paradoxical sleep?].		1984	185591
restriction endonuclease mapping and cloning of mycobacterium fortuitum var. fortuitum plasmid pal5000.	a restriction map of mycobacterium fortuitum var. fortuitum plasmid pal5000 was established. the unique sites for apai, bamhi, bglii, bsteii, clai, ecori, ecorv, hpai, kpni and nari were located on the 5.0-kb plasmid. the plasmid had no sites for ahaiii, bcli, hindiii, psti, sphi and xbai. pal5000 was cloned into pbr322 and propagated in escherichia coli. three hybrid pal5000-pbr322 plasmids carrying the complete pal5000 sequence were constructed by joining the plasmids at their bamhi, ecori or ...	1985	3002238
restriction endonuclease mapping and cloning of mycobacterium fortuitum var. fortuitum plasmid pal5000.	a restriction map of mycobacterium fortuitum var. fortuitum plasmid pal5000 was established. the unique sites for apai, bamhi, bglii, bsteii, clai, ecori, ecorv, hpai, kpni and nari were located on the 5.0-kb plasmid. the plasmid had no sites for ahaiii, bcli, hindiii, psti, sphi and xbai. pal5000 was cloned into pbr322 and propagated in escherichia coli. three hybrid pal5000-pbr322 plasmids carrying the complete pal5000 sequence were constructed by joining the plasmids at their bamhi, ecori or ...	1985	3002238
rocky mountain spotted fever in dogs and its differentiation from canine ehrlichiosis.	rocky mountain spotted fever (rmsf) or ehrlichiosis was diagnosed in dogs on the basis of specific immunofluorescent testing for each disease. comparisons between clinical and laboratory findings were made between the 2 diseases. the incidence of rmsf tended to be more seasonal and it affected younger dogs. purebred dogs appeared to be more susceptible to both diseases. in general, rmsf had a more rapid and severe course of clinical illness than did ehrlichiosis, but acute ehrlichiosis was diffi ...	1985	3972706
energy and protein relationships in the broiler. 1. effect of protein levels and feeding regimens on growth, body composition, and in vitro lipogenesis of broiler chicks.	male broiler chicks were fed ad libitum diets containing 18, 23, or 30% protein for 3 weeks. in addition, chicks were fed diets containing 12, 23, or 30% protein on a 3-day rotation. finally, chicks were fed either the 23 or 30% protein on a schedule of 2 days on feed and 1 day off feed. these feeding schemes allowed chicks to consume low to excess protein coupled with either adequate or inadequate energy intake. feed intakes were equal for the 18, 23, and rotational percent protein dietary grou ...	1985	3975192
contact urticaria from diethyl fumarate.	the contact urticariagenic properties of diethyl fumarate were studied using the guinea pig ear swelling assay and by open application on the human upper back skin. diethyl fumarate caused non-immunologic contact urticaria in both human and guinea pig skin, and the reactions exhibited similar dose dependency and timing of maximal response.	1985	3995941
canine rocky mountain spotted fever: a kennel epizootic.	within a period of 5 consecutive days after the initial observation of illness was made, 7 of 12 siberian husky dogs developed clinical signs of rickettsia rickettsii infection. one dog died and was necropsied. clinical signs of infection consisted of lethargy, anorexia, ocular and nasal discharges, and neurologic disorder (incoordination and rolling). scleral blood vessel injection, fever, lymphadenomegaly, splenomegaly, and increased bronchovesicular lung sounds were prominent findings. clinic ...	1985	4062017
[modifications in relation to age in the number and diameter of nerve fibers in dorsal spinal roots of the rat].		1985	4062475
[effects of thymosin on the humoral immune response to antibody-dependent cell cytotoxicity].		1985	4065002
rocky mountain spotted fever mimicking acute cholecystitis.	rocky mountain spotted fever can present with predominantly abdominal symptoms including nausea, vomiting, diarrhea, and abdominal pain. two elderly patients presented with an acute febrile illness and abdominal symptoms. rash was not present initially. workup disclosed cholelithiasis in one, and a thickened gallbladder wall surrounded by a sonolucent zone suggesting a pericholecystic abscess was found by ultrasonography in the other. both patients underwent emergency laparotomy, with cholecyste ...	1985	4074033
a study of intrathecal self-injection of morphine by rats, and the difficulties entailed.	rats were chronically implanted with an intrathecal cannula, through which they were taught to self-administer morphine. after a training period of 2 weeks, their self-injection behaviour was studied before, during and after nociceptive stimulation. in response to nociceptive stimulation the rats increased their intrathecal self-injection of morphine. this effect is specific for morphine, since under the same conditions rats did not increase nacl self-injection. acute naloxone pre-treatment was ...	1985	3840877
the medical profession and nuclear war. a social history.	since world war ii, individual physicians and medical organizations in the united states have cooperated with the federal government in preparing for nuclear war. while most physicians have maintained a neutral stance, a minority have resisted federal policies. health professionals participated actively at the wartime laboratories that developed the atomic bomb and in the medical research that followed hiroshima and nagasaki. professional organizations helped with civil defense planning for nucl ...	1985	3892071
gastric inhibitory polypeptide and hyperinsulinemia in the zucker (fa/fa) rat: a developmental study.	the role of the gut hormone gip (gastric inhibitory polypeptide; glucose-dependent insulinotropic polypeptide) in the development of hyperinsulinemia of pre-obese (fa/fa) zucker rats was investigated. plasma gip levels were compared in lean and fa/fa pups from 21 to 35 days of age. the onset of both basal and glucose-stimulated hyperinsulinemia was studied. possible causal roles for glucose, gip and acetylcholine in hyperinsulinemia were investigated in the isolated perfused pancreas preparation ...	1985	3897081
glutaminyl-trna synthetase of escherichia coli.		1985	3911010
serological evidence of subclinical rocky mountain spotted fever infections in texas.		1985	3918125
rocky mountain spotted fever in children in kansas: the diagnostic value of an igm-specific immunofluorescence assay.		1985	3918126
the prevalence of antibodies to rickettsia rickettsii in an area endemic for rocky mountain spotted fever.	a study of rickettsia rickettsii was conducted in rowan, cabarrus, and granville counties, north carolina in an attempt to define the prevalence of endemic infection in this area. serum samples were obtained from 1,976 healthy persons and tested by indirect hemagglutination for detectable antibodies to r. rickettsii. of this group, 568 (28.7%) had detectable antibody (greater than or equal to 1:8), 80 (4%) had titers greater than or equal to 1:64, and 1,408 (70%) had no detectable antibody (less ...	1985	3921622
characterization of monoclonal antibodies protecting mice against rickettsia rickettsii.	hybridomas producing monoclonal antibodies to rickettsia rickettsii were prepared from mice to investigate the function of rickettsial antigens. of the 31 reactive hybridoma lines thus far tested for immunoglobulin subclasses, 11 belonged to the igg2a subclass, 9 to the igg2b subclass, and 7 to the igg3 subclass; four did not react with any of the isotyping sera. five of the antibodies recognized epitopes present on molecules that were presumed to be polysaccharide and heterogeneous in molecular ...	1985	3923129
rickettsia-like organisms in ticks and antibodies to spotted fever-group rickettsiae in mammals from northern mississippi.	studies were conducted from april through august during 1974 and 1975 on 30 randomly selected trapping sites in wall doxey state park, holly springs national forest, and 1.6 km south of the park in marshall county, mississippi to provide information on the occurrence of ticks involved in the maintenance and transmission of spotted fever-group rickettsiae in nature. of 365 animals (14 species) collected, 186 (51%) were parasitized by 4,169 ticks. species of ticks collected included: dermacentor v ...	1985	3923212
isolation and identification of rickettsia rickettsii from the rabbit tick (haemaphysalis leporispalustris) in the atlantic zone of costa rica.	during the ecological studies done in costa rica on rocky mountain spotted fever, we have been able to isolate 2 strains of rickettsiae from the rabbit tick haemaphysalis leporispalustris, indistinguishable from r. rickettsii isolated from humans in our country. moreover, serological evidence of this agent was demonstrated in convalescent guinea pigs and in the sera of the wild rabbit (sylvilagus braziliensis).	1985	3923853
[preliminary studies on the occurrence of rickettsiae of the spotted fever group in rhipicephalus sanguineus captured in suburban areas].	in the urban periphery of rome, two sub-zones could be distinguished, characterized by uncultivated steppeland and by small shacks with large quantities of domestic refuse, respectively. rhipicephalus sanguineus appeared to be typical of this second sub-zone. r. sanguineus adults and nymphae were captured in different seasonal periods and were checked for the prevalence of rickettsiae of the spotted fever (sf) group. moreover, larvae from hatched eggs, laid by captured r. sanguineus females, wer ...	1985	3924079
ultrastructural changes observed in rickettsia rickettsii incubated at different temperatures in cell-free medium.	when cells of rickettsia rickettsii were suspended at different temperatures in growth medium free of host cells, ultrastructural changes were observed in some of these organisms. depending upon the temperature and length of incubation, loosely organized cells developed into compact, intensely stained, rod-shaped organisms. these compact cells closely resembled the morphology of the original culture of rickettsia used for inoculation. morphological changes were primarily noted in cells maintaine ...	1985	3924384
diurnal host seeking of adult pacific coast ticks, dermacentor occidentalis (acari: ixodidae), in relation to vegetational type, meteorological factors, and rickettsial infection rates in california, usa.		1985	3930742
developmental sequence and surface membrane assembly of rickettsiae.		1985	3933409
spotted fever group rickettsiosis in japan.	the acute febrile disease with characteristic rash encountered in tokushima prefecture was proved to be a spotted fever group rickettsiosis, which showed a significant rise in agglutinins to both proteus ox2 and ox19 and significantly high levels of cf antibodies to rickettsia akari and rickettsia rickettsii.	1985	3934436
pulmonary pathology of rocky mountain spotted fever (rmsf) in children.	despite infrequent respiratory symptoms, histopathologic changes were identified in the lungs of 15 of 16 children dying of rocky mountain spotted fever (rmsf). gross examination demonstrated increased lung weight, edema, congestion, focal hemorrhage, and bronchopneumonia in a few cases. paraffin sections were stained with hematoxylin and eosin and phosphotungstic acid-hematoxylin, and available blocks were examined by direct immunofluoresence for rickettsia rickettsii. cases and controls matche ...	1985	3938014
spotted fever group rickettsiae in immature and adult ticks (acari: ixodidae) from a focus of rocky mountain spotted fever in connecticut.	immature and adult ixodid ticks were collected during 1983 and 1984 in newtown, connecticut, an area endemic for rocky mountain spotted fever (rmsf), to determine prevalence of infection by spotted fever group (sfg) rickettsiae. direct fluorescent-antibody (fa) staining revealed sfg organisms in 6 (1.8%) of 332 dermacentor variabilis larvae, 5 (7.8%) of 64 d. variabilis nymphs, and in 2 (40%) of 5 ixodes cookei nymphs removed from small- and medium-sized mammals. hemolymph tests detected rickett ...	1985	3938341
[evaluation of weil-felix test results by comparing the agglutinability of proteus ox2 and ox19 commercial antigens: basis for the presumption of a spotted fever group rickettsiosis].		1985	3938464