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an outbreak of infections with acinetobacter calcoaceticus in burn patients: contamination of patients' mattresses.during a 21-month period acinetobacter calcoaceticus was the most common organism causing infections in a university burn center. forty-three of 103 patients admitted became infected with this organism. risk factors associated with burn wound colonization with acinetobacter included larger burns and foley catheter use; however, only a longer duration of hospitalization was an independent discriminator of colonization. infection-control measures, including strict isolation and closure and repaint ...19853968451
isolation of acinetobacter calcoaceticus from a cow with mastitis. 19853976314
assessment of head-space gas-liquid chromatography for the rapid detection of growth in blood cultures.blood for transfusion was inoculated with between 10(0) and 10(2) colony-forming units (cfu) per ml of each of 59 microbial isolates and added to cooked meat broth. at intervals up to 72 h incubation, the cultures were examined by conventional visual inspection and automated head-space gas-liquid chromatography (hs-glc). forty-six isolates including all those examined of staphylococcus aureus, streptococcus pyogenes, s. pneumoniae, s. faecalis, s. milleri, s. mitior, s. mitis, s. salivarius, s. ...19853988854
transferable amikacin resistance in pseudomonas maltophilia and acinetobacter calcoaceticus. 19853997255
enzymatic profile of clinical isolates of acinetobacter calcoaceticus.the enzymatic profiles of 109 clinical isolates of acinetobacter calcoaceticus subsp. anitratus and lwoffi were determined with conventional plate tests and the rapid api zym system (analytab products, plainview, n.y.). the majority of strains tested lacked dnase, hemolysin, protease, elastase and gelatinase. strong enzymatic activities of butyrate esterase (c4), caprylate esterase (c8) and leucine arylamidase were detected in all isolates. no trypsin, chymotrypsin, alkaline phosphatase or gluco ...19854000064
acinetobacter calcoaceticus septicemia in patients with cancer.at our institution, 95 cases of acinetobacter septicemia occurred over a ten-year period (1973 to 1982) in patients being treated for cancer. in 24 patients the infection was polymicrobial, while acinetobacter ssp was the only offending pathogen in 71 patients. in 76 patients (80%), the infection was related to an indwelling central venous catheter (cvc). a sharp increase in the frequency of acinetobacter septicemia was noticed in the years 1981 and 1982 and coincided with a marked increase in t ...19854001997
in-vitro and in-vivo activities of antimicrobial agents against acinetobacter calcoaceticus.the virulence of clinical isolates of acinetobacter calcoaceticus subsp. anitratus was studied in mice, and in-vitro and in-vivo activities of several antimicrobial agents were evaluated. in in-vitro susceptibility testings, tetracyclines, aminoglycosides, and peptide were highly sensitive, and minocycline and doxycycline were the most active of 21 antibiotics tested against 84 clinical isolates of acin. calcoaceticus. virulence tests for mice revealed that some strains exhibited high virulence ...19854008377
[utilization of 3-chlorobenzoic acid by acinetobacter calcoaceticus].a strain of acinetobacter calcoaceticus assimilating 3-chlorobenzoic acid (3-cba) as a sole source of carbon and energy was isolated from soil near moscow. when 3-cba is utilized, 2-chloro-cis, cis-muconic acid is accumulated in the medium. the liberation of chlorine atoms is 50--60% of the theoretically possible value. the oxidation of 3-cba yields 3-chloropyrocatechol (3-cpc) and 4-chloropyrocatechol (4-cpc). 4-cpc serves as a source of nutrition for a. calcoaceticus. the oxidation of 3-cpc yi ...19854010553
[acinetobacter calcoaceticus infection--bacteriological and clinical studies of septicemia]. 19854032800
regions of broad-host-range plasmid rk2 involved in replication and stable maintenance in nine species of gram-negative bacteria.the replication and maintenance properties of the broad-host-range plasmid rk2 and its derivatives were examined in nine gram-negative bacterial species. two regions of rk2, the origin of replication (oriv) and a segment that encodes for a replication protein (trfa delta kild, designated trfa*), are sufficient for replication in all nine species tested. however, stable maintenance of this minimal replicon (less than 0.3% loss per generation under nonselection conditions) is observed only in esch ...19854044529
replacement of methoxatin by 4,7-phenanthroline-5,6-dione and the inability of other phenanthroline quinones, as well as 7,9-di-decarboxy methoxatin, to serve as cofactors for the methoxatin-requiring glucose dehydrogenase of acinetobacter calcoaceticus.glucose dehydrogenase from a. calcoaceticus has been dissociated into apoenzyme and methoxatin coenzyme, and enzyme activity restored by replacing coenzyme with 4,7-phenanthroline-5,6-dione but not with 1,10- nor 1,7-phenanthroline-5,6-diones nor with 7,9-decarboxy methoxatin.19854052066
synergistic necrotizing fasciitis: a case of polymicrobic infection with acinetobacter calcoaceticus. 19854053677
structural studies of the capsular polysaccharide of acinetobacter calcoaceticus bd4.compositional analysis of the intact and carboxyl-reduced capsular polysaccharide of acinetobacter calcoaceticus bd4 (ps-4) showed it to consist of l-rhamnose, d-glucose, d-glucuronic and d-mannose in molar ratios of 4:1:1:1. 13c-nuclear magnetic resonance spectroscopy, methylation analysis, oligosaccharide analysis and base-catalyzed beta-elimination were used to elucidate the primary structure. oligosaccharides were obtained by enzymatic depolymerization with a specific bacteriophage-induced d ...19854054115
a 28,000-dalton protein of normal mouse serum binds specifically to the inner core region of bacterial lipopolysaccharide.normal mouse serum was found to contain a protein, referred to here as factor, which binds to the inner core region of lipopolysaccharides (lpss) of various bacterial families. since factor-lps interactions resulted in activation of guinea pig complement, factor activity could be assayed by a passive hemolysis test with sheep erythrocytes coated with lps or lipid a from acinetobacter calcoaceticus (which was found earlier to bind particularly well to factor). factor was purified by g-50 and hydr ...19854066028
endemic nosocomial acinetobacter calcoaceticus bacteremia. clinical significance, treatment, and prognosis.the medical records of 27 patients with blood cultures positive for acinetobacter calcoaceticus over a recent five-year period (0.7% of all positive blood cultures) were reviewed retrospectively to determine the epidemiologic and clinical significance of these isolates. eighteen isolates represented true bacteremias, 16 of which were hospital acquired. patients most frequently were located in an intensive care unit or on a surgical ward. a seasonal july-to-september peak incidence was noted. the ...19854074029
identification of 2-keto-3-deoxy-1,7-dicarboxyheptonic acid as a constituent of the lipopolysaccharide of acinetobacter calcoaceticus nctc 10305.a 3-deoxyaldulosonic acid, not previously described, was identified as a component of the lipopolysaccharide of acinetobacter calcoaceticus nctc 10305: 2-keto-3-deoxy-1,7-dicarboxyheptonic acid. this compound, whose steric configuration has not been determined, was released from lipopolysaccharide after acid hydrolysis as monosaccharide and in the form of a glucose-substituted disaccharide. it was identified by combined gas-liquid chromatography/mass spectrometry using various derivatization pro ...19854076175
an underestimated nosocomial pathogen, acinetobacter calcoaceticus. 19854077770
[comparison of the in vitro activity of amikacin, netilmicin, piperacillin and moxalactam against acinetobacter calcoaceticus var. anitratum]. 19854079560
interconvertible molecular-weight forms of the bifunctional chorismate mutase-prephenate dehydratase from acinetobacter calcoaceticus.acinetobacter calcoaceticus belongs to a large phylogenetic cluster of gram-negative procaryotes that all utilize a bifunctional p-protein (chorismate mutase-prephenate dehydratase) [ec 5.4.99.5-4.2.1.51] for phenylalanine biosynthesis. these two enzyme activities from ac. calcoaceticus were inseparable by gel-filtration or deae-cellulose chromatography. the molecular weight of the p-protein in the absence of effectors was 65,000. in the presence of l-tyrosine (dehydratase activator) or l-phenyl ...19854083897
versatile mercury-resistant cloning and expression vectors.cloning vectors have been constructed employing two diverse replicons, incq and p15a. both vectors confer resistance to kanamycin (km) and mercuric ions (hg2+). one of these vectors, pdg105, is a broad-host-range, nonconjugative, oligocopy incq plasmid, which is capable of transforming escherichia coli, acinetobacter calcoaceticus, and pseudomonas putida. the second vector, pdg106, is a narrow-host-range, multicopy cloning vector compatible with pbr322. both vectors contain unique cloning sites ...19854092936
exocellular esterase and emulsan release from the cell surface of acinetobacter calcoaceticus.an esterase activity has been found, both in the cell-free growth medium and on the cell surface of the hydrocarbon-degrading acinetobacter calcoaceticus rag-1. the enzyme catalyzed the hydrolysis of acetyl and other acyl groups from triglycerides and aryl and alkyl esters. emulsan, the extracellular heteropolysaccharide bioemulsifier produced by strain rag-1, was also a substrate for the enzyme. gel filtration showed that the cell-free enzyme was released from the cell surface either emulsan fr ...19853838301
tolerance of acinetobacter calcoaceticus rag-1 to the cationic surfactant cetyltrimethylammonium bromide: role of the bioemulsifier emulsan.emulsan, the polyanionic heteropolysaccharide bioemulsifier produced by acinetobacter calcoaceticus rag-1, was found to enhance the tolerance of rag-1 cells to the toxic effects of the cationic detergent cetyltrimethylammonium bromide (ctab). emulsan-mediated tolerance was obtained with the purified deproteinated apoemulsan; ca. 9 micrograms of apoemulsan neutralized 1 microgram of ctab. deesterified apoemulsan was only half as effective in protecting the cells from ctab toxicity. tolerance was ...19853838426
evaluation of antibiotic susceptibility testing by agar dilution and the micro media system (fox panel).the susceptibilities of 350 gram-positive cocci and 638 gram-negative bacilli to various antimicrobial agents were compared by using the micro-media system (mms) (fox panel) (micro-media systems, inc., potomac, md.) and a standard agar dilution procedure. major discrepancies occurred with enterococci, among which 48 of 53 isolates (91%) were found to be resistant to penicillin g by agar dilution and reported as susceptible by the mms. other large discrepancies occurred with staphylococcus aureus ...19853850093
plasmid transfer and behaviour in acinetobacter calcoaceticus ebf65/65.at least one plasmid from each of the incompatibility groups b, c, fiv, h2/s, i alpha, i delta, p, w and x was shown to be capable of transfer from escherichia coli k12 to acinetobacter calcoaceticus ebf65/65. transfer was influenced by the presence of pav2 (thought to encode a restriction-modification system) in the recipient strain; however, not all plasmids belonging to a particular incompatibility group behaved identically. all plasmids were unstable to varying degrees in a. calcoaceticus eb ...19853851820
aztreonam in the treatment of serious orthopedic infections.aztreozam was evaluated in the treatment of a variety of orthopedic infections. included were 17 patients with osteomyelitis, three with purulent arthropathy with prostheses, and 16 with superficial infections secondary to trauma or surgical procedure. pathogens were gram-negative bacilli sensitive to aztreonam. concomitant antibiotics were administered for gram-positive cocci that were present initially or by superinfection. infecting organisms included pseudomonas aeruginosa (minimal inhibitor ...19853881949
membrane-bound lactate dehydrogenases and mandelate dehydrogenases of acinetobacter calcoaceticus. location and regulation of expression.acinetobacter calcoaceticus possesses an l(+)-lactate dehydrogenase and a d(-)-lactate dehydrogenase. results of experiments in which enzyme activities were measured after growth of bacteria in different media indicated that the two enzymes were co-ordinately induced by either enantiomer of lactate but not by pyruvate, and repressed by succinate or l-glutamate. the two lactate dehydrogenases have very similar properties to l(+)-mandelate dehydrogenase and d(-)-mandelate dehydrogenase. all four e ...19853890833
[lack of specificity of protected distal bronchial samples in the bacteriological diagnosis of nosocomial pneumonia caused by gram negative bacilli].lower respiratory tract secretions (lrs) were sampled during a two year period in patients undergoing mechanical ventilation using a method previously described by matthew et al. only those lrs positive for gram-negative bacilli were reported in this study. the patients were separated in two groups: those with nosocomial pneumonia due to gram-negative bacilli (np+ group) and those without (np- group). the species most often isolated from lrs were: pseudomonas aeruginosa (25%), proteus (20%), esc ...19853893235
[carbenicillin resistance of gram-negative bacteria: incidence, biochemical and genetic determinism].of nine hundred ampicillin resistant (amp-r) enterobacteria strains, isolated in hospital between july and december 1981, 73,7% are also carbenicillin-resistant (carb-r). this particular double resistance varies depending upon the species considered: indole positive proteus (23%), enterobacter cloacae (64%), citrobacter freundii (67%), acinetobacter calcoaceticus (73%), proteus mirabilis (75%), serratia marcescens (90%), escherichia coli (91%), providencia stuartii (96%) and klebsiella pneumonia ...19853903620
membrane-bound lactate dehydrogenases and mandelate dehydrogenases of acinetobacter calcoaceticus. purification and properties.procedures were developed for the optimal solubilization of d-lactate dehydrogenase, d-mandelate dehydrogenase, l-lactate dehydrogenase and l-mandelate dehydrogenase from wall + membrane fractions of acinetobacter calcoaceticus. d-lactate dehydrogenase and d-mandelate dehydrogenase were co-eluted on gel filtration, as were l-lactate dehydrogenase and l-mandelate dehydrogenase. all four enzymes could be separated by ion-exchange chromatography. d-lactate dehydrogenase and d-mandelate dehydrogenas ...19853904742
solubilization of bacterial cells in organic solvents via reverse micelles.a reverse micellar system containing tween 85 and water in isopropylpalmitate was developed which permitted the solubilization of bacteria in the form of homogenous organic solutions. the presence of the bacteria in solution was demonstrated by light microscopy. immediately after solubilization, isolated bacterial cells were observed, which by aging tend to form larger aggregates. cells of escherichia coli remained viable in this system for at least one day and retained beta-galactosidase activi ...19853921020
anti-pseudomonal activity of hr 810.the anti-pseudomonal activity of hr 810, a new 2-aminothiazolyl cephalosporin, was compared to that of carbenicillin, azlocillin and cefsulodin against 187 non-fermenters. hr 810 was the best agent against pseudomonas aeruginosa, pseudomonas putida, pseudomonas fluorescens and acinetobacter calcoaceticus with an mic50 less than or equal to 4 mg/l and an mic90 less than or equal to 16 mg/l. it was as effective as azlocillin against pseudomonas stutzeri and pseudomonas mendocina, with an mic50 les ...19853922898
comparative in vitro activities of cefpiramide and apalcillin individually and in combination.the in vitro activities of cefpiramide and apalcillin were compared with those of other third-generation cephalosporins and extended-spectrum penicillins against over 1,000 clinical bacterial isolates. the activity of cefpiramide against pseudomonas aeruginosa was comparable to those of piperacillin and cefoperazone, inhibiting 90% of strains at concentrations less than or equal to 16.0 micrograms/ml. this drug was also active against a broad range of gram-negative organisms but was generally le ...19853925875
energy transduction by electron transfer via a pyrrolo-quinoline quinone-dependent glucose dehydrogenase in escherichia coli, pseudomonas aeruginosa, and acinetobacter calcoaceticus (var. lwoffi).the coupling of membrane-bound glucose dehydrogenase (ec 1.1.99.17) to the respiratory chain has been studied in whole cells, cell-free extracts, and membrane vesicles of gram-negative bacteria. several escherichia coli strains synthesized glucose dehydrogenase apoenzyme which could be activated by the prosthetic group pyrrolo-quinoline quinone. the synthesis of the glucose dehydrogenase apoenzyme was independent of the presence of glucose in the growth medium. membrane vesicles of e. coli, grow ...19853926746
antibacterial activity in vitro and regression studies for ceftazidime and ceftriaxone.the antibacterial activity in vitro of ceftazidime and ceftriaxone was investigated against 575 recent clinical isolates. both cephalosporins displayed excellent activity against most of the pathogens tested, in particular gram-negative bacteria, including pseudomonas aeruginosa. apart from the pseudomonas group, acinetobacter calcoaceticus and campylobacter jejuni ceftriaxone was slightly to moderately more active than ceftazidime overall. ceftriaxone was moderately active against streptococcus ...19853929558
nucleotide sequence, secondary structure and evolution of the 5s ribosomal rna from five bacterial species.the nucleotide sequences of the 5s ribosomal rnas of the bacteria agrobacterium tumefaciens, alcaligenes faecalis, pseudomonas cepacia, aquaspirillum serpens and acinetobacter calcoaceticus have been determined. the sequences fit in a generally accepted model for 5s rna secondary structure. however, a closer comparative examination of these and other bacterial 5s rna primary structures reveals the potential of additional base pairing and of multiple equilibria between a set of slightly different ...19852408888
epidemic spread of acinetobacter calcoaceticus in a neurosurgical department analyzed by electronic data processing.an epidemic spread of acinetobacter calcoaceticus var. anitratus in two neurosurgical wards is described retrospectively and prospectively using electronic data processing. isolation of the species from sputum preceded the isolation from csf by 1/2-1 year. control measures directed against spread by air and indirect contact controlled the epidemic. reexamination of 20 selected strains from the epidemic revealed two distinct resistance patterns.19852862191
molecular analysis of an antibiotic resistance plasmid, pav5, and its derivative plasmids in acinetobacter calcoaceticus.the non-conjugative plasmid pav5 specifies resistance to kanamycin/neomycin (kmr) and tetracycline (tcr). physical evidence is presented to show that pav5 gives rise to two plasmids, pav51 (kmr) and pav52 (tcr), which are formed by deletion of apparently non-overlapping segments of pav5. expression of tcr has been obtained in escherichia coli and is associated with a 1.9 kb hindiii fragment found in pav5 and in pav52. expression of kmr has been obtained in e. coli and is associated with a 1.3 kb ...19853007656
acinetobacter calcoaceticus encoded mutarotase: nucleotide sequence analysis of the gene and characterization of its secretion in escherichia coli.the nucleotide sequence of the mutarotase gene from acinetobacter calcoaceticus has been determined. it reveals an open reading frame of 381 amino acids. the codon usage of a. calcoaceticus for this gene is similar to e. coli except for the amino acids leu, ala, glu, and arg where major differences exist. this did not interfere drastically with high level expression in e. coli. the regulatory sequences for the initiation of translation are similar to the ones described for e. coli. the n-termina ...19863012466
molecular cloning into tn5 and integration in the pseudomonas aeruginosa chromosome: a tool for heterologous gene expression.the dna primase gene of the promiscuous incp-1 conjugative plasmid rp1, encoding two polypeptides of 118 and 80 kda, was inserted into the transposon tn5 in escherichia coli. the derivative transposon, tn2523, was then transposed to a temperature-sensitive replication mutant of the promiscuous incp-1 conjugative plasmid r68 at permissive temperature and the plasmid transferred to pseudomonas aeruginosa strain pao. the latter strain was then grown at non-permissive temperature to identify transpo ...19863016149
the oxidation of glucose by acinetobacter calcoaceticus: interaction of the quinoprotein glucose dehydrogenase with the electron transport chain.the coupling of the quinoprotein glucose dehydrogenase to the electron transport chain has been investigated in acinetobacter calcoaceticus. no evidence was obtained to support a previous suggestion that the soluble form of the dehydrogenase and the soluble cytochrome b associated with it are involved in the oxidation of glucose. analysis of cytochrome content, and of reduction of cytochromes in membranes by substrates, and of sensitivity to cyanide indicated that glucose, succinate and nadh are ...19863021895
polymyxin-b inhibition of lps-induced interleukin-1 secretion by human monocytes is dependent upon the lps origin.polymyxin-b (pmb) is an antibiotic known to inhibit various biological activities induced by lipopolysaccharides (lps). we have investigated the ability of pmb to inhibit lps-induced interleukin-1 (il-1) secretion by human monocytes in vitro. interleukin-1 was assayed by the conventional comitogenic assay using mice thymocytes. our data demonstrate that pmb (1-2 micrograms/assay)-mediated inhibition of lps-induced il-1 secretion depends on the origin of the lps. interleukin-1 secretions induced ...19863023974
sulbactam/ampicillin: in vitro spectrum, potency, and activity in models of acute infection.more than 90% of community hospital-isolated strains of staphylococcus (including methicillin-resistant isolates), streptococcus, haemophilus, neisseria, branhamella, bacteroides, escherichia coli, klebsiella, enterobacter aerogenes, proteus, and acinetobacter calcoaceticus were inhibited by the sulbactam/ampicillin (1:2) combination at concentrations of 8 micrograms/16 micrograms per ml. the peak serum level from a 15-min infusion of 1 g/2 g of sulbactam/ampicillin is more than seven times this ...19863026001
investigation of an outbreak of infection with acinetobacter calcoaceticus in a special care baby unit.during a period of 6 months, an 'epidemic strain' of acinetobacter calcoaceticus was isolated from 10 pre-term neonates in a special care baby unit (scbu). of these, nine had pulmonary infections. the tenth was found to have conjunctival colonization only. the 'epidemic strain' was characterized by serotyping, biotyping, bacteriocin typing and antibiograms. an identical strain was isolated from an 'ambu' resuscitation device but not from other environmental samples or from staff on the unit. the ...19862870108
localization of hydrolytic enzymes in acinetobacter calcoaceticus.the localization of hydrolytic enzymes, phosphatase, esterase, lipase and palmitoyl-coa hydrolase was analysed in the cytosol, cytoplasmic membrane, periplasmic fraction, outer membrane and culture supernatant in dependence on the growth rate of the bacteria. the unspecific phosphatase was found to be a cytosolic enzyme. a lipase was the only extracellular enzyme detected. the results pointed to a secretion of the lipase into the culture medium via cytoplasmic and outer membrane. the palmitoyl-c ...19862870172
defence reactions of glossina morsitans morsitans against different species of bacteria and trypanosoma brucei brucei.tsetse flies, glossina morsitans morsitans, fed on rats infected with trypanosoma brucei brucei showed wide fluctuations in total and differential haemocyte counts. similar fluctuations occurred in controls fed on non-infected rats and also between the two groups without showing any difference which could be attributed to the infection. trypanosome infection of the tsetse haemocoel occurred in 16.25% of the flies, starting from the second day after feeding on the infected rats, but salivary glan ...19862872785
transformation and mobilization of cloning vectors in acinetobacter spp.r300b-, rsf1010-, and rk2-derived plasmids were introduced into acinetobacter sp. strain ho1-n and acinetobacter calcoaceticus bd413 by transformation and conjugal mobilization. the transformation frequencies of bd413 were 4.2 x 10(6) to 6.3 x 10(6) transformants per micrograms of dna per 10(9) recipient cells. conjugal mobilization frequencies were 1.1 x 10(-1) to 8.5 x 10(-1) per recipient. an improved method for the transformation of a. calcoaceticus bd413 is reported.19863941044
effect of oxygen limitation on the content of n-hexadecane-inducible cytochrome p-450 in acinetobacter calcoaceticus strain eb 104.the content of cytochrome p-450 as a function of oxygen supply was studied during growth of acinetobacter on n-hexadecane in batch cultures at constant ph and agitation. the rate of growth and the content of cytochrome p-450 were not affected as long as the dissolved oxygen tension ranged above 3 to 5% of saturation. the amount of cytochrome p-450 increased when the oxygen tension declined to zero. cytochrome p-450 levels of about 0.3 to 0.4 nmol/mg protein, i.e. a more than a threefold increase ...19863585713
prophage induction by ultraviolet light in acinetobacter calcoaceticus.uv-induction of prophage p78 of acinetobacter calcoaceticus increased with the uv-dose given to the lysogenic strain from the spontaneous induction frequency of about 0.8% to a maximal frequency of 10%. this 10- to 20-fold increase of induction frequency, as measured by the number of infective centres, was accompanied by a 1000-fold increase in the yield of free phage. this effect was probably due to an increase in burst size under the conditions of lysogenic induction. unusually, the lysogen wa ...19863794658
isolation of acinetobacter calcoaceticus from cows with metritis. 19863799102
in vitro susceptibility of acinetobacter species to various antimicrobial agents.the in vitro activity of 18 antimicrobial agents against 40 clinical isolates each of acinetobacter calcoaceticus subsp. anitratum and acinetobacter lwoffi was studied. most of the newer 4-quinolone derivatives were extremely active against these organisms. newer beta-lactam agents, such as cefpirome, bmy 28142, and brl 36650, were also extremely active, inhibiting all strains at clinically achievable levels. most agents were two- to fourfold more active against a. lwoffi.19863800353
purification and characterization of quinoprotein glucose dehydrogenase from acinetobacter calcoaceticus l.m.d. 79.41.quinoprotein glucose dehydrogenase (ec 1.1.99.17) from acinetobacter calcoaceticus l.m.d. 79.41 was purified to homogeneity. it is a basic protein with an isoelectric point of 9.5 and an mr of 94,000. denaturation yields two molecules of pqq/molecule and a protein with an mr of 48000, indicating that the enzyme consists of two subunits, which are probably identical because even numbers of aromatic amino acids were found. the oxidized enzyme form has an absorption maximum at 350 nm, and the reduc ...19863800975
comparative activity of imipenem, ceftazidime and cefotaxime against acinetobacter calcoaceticus.imipenem was the most active drug against acinetobacter, even against strains possessing beta-lactamases. mics of imipenem for 50% and 90% of the a. calcoaceticus (var. anitratum) were 0.28 and 0.61 mg/l respectively whereas for ceftazidime they were 4.4 and 10.0, and for cefotaxime, 7.9 and 22.2 mg/l. no change occurred in the in-vitro activity of imipenem against acinetobacter, during the period 1980-1985. geometric mean mics in 1985 were 0.33 mg/l for a. calcoaceticus var. anitratum; the valu ...19863546246
postantibiotic effect of imipenem on gram-positive and gram-negative micro-organisms.the bactericidal effect (be) of an antibiotic reduces the infective population, and its postantibiotic effect (pae) assures a persistent inhibition of bacterial cells after a short exposure to the antimicrobial agent. both effects prevent the early regrowth of the infecting organisms when the antibiotic tissue levels decrease to below the mic value. the be and the pae of imipenem, cefotaxime, ceftazidime, piperacillin, gentamicin and ampicillin on escherichia coli, klebsiella pneumoniae, enterob ...19863546247
evolved aniline catabolism in acinetobacter calcoaceticus during continuous culture of river water.adaptation of acinetobacter calcoaceticus from river water to aniline depends on the dynamics of parent and mutant populations. the parent, acinetobacter strain don26 phenotype ani0, was common in river water and assimilated aniline effectively at micromolar concentrations, but was inhibited at higher concentrations of aniline. the ani0 phenotype was also characterized by a broad specificity for oxidation of chloroanilines by aniline-induced cells. the mutant ani+ phenotype was represented by do ...19863707123
[temperature dependence of membrane-bound aldehyde dehydrogenase from acinetobacter: effect of solubilization and chain length of the substrates].the nadp+-dependent aldehyde dehydrogenase of intracytoplasmic membranes of acinetobacter calcoaceticus shows a characteristic behaviour of its reaction velocities in dependence on temperature. the calculated "real" activation energies depend on the increasing chain-length of the homologous aldehydes and grow appropriate to the membrane-bound and to the micellar form of the enzyme with 6.2 and 7.3 kj/mole ch2-group, respectively. the values of the activation energy of the membrane-bound enzyme a ...19863707551
acinetobacter calcoaceticus in three cases of late abortion in water buffaloes. 19863716168
variation in composition and yield of exopolysaccharides produced by klebsiella sp. strain k32 and acinetobacter calcoaceticus bd4.the exopolysaccharides produced by klebsiella sp. strain k32 and acinetobacter calcoaceticus bd4 under different growth conditions have been analyzed for sugar composition. the first use of ion chromatography for the quantitative determination of microbial exopolysaccharide composition is reported. klebsiella sp. strain k32 produced a polymer composed of rhamnose, galactose, and mannose early in its fermentation. the composition of the polymer varied markedly depending on the growth stage of the ...19863729401
emulsifier formation with acinetobacter: search for an excretion-reduced mutant of acinetobacter calcoaceticus 69-v.after growth on acetate three groups of acinetobacter strains could be identified with respect to the excretion of a bioemulsifier. mutants of a. calcoaceticus 69-v were selected which produced reduced amounts of emulsifier.19863746629
[aerobic treatment of pig slurry: microbial aspects].during the treatment of raw pig slurry by batch aerobic fermentation, the presence of surfaces for bacterial attachment resulted in a 1,000-fold increase in the microbial population. in the absence of such surfaces, a 65% reduction of the chemical oxygen demand (cod) of the manure was obtained after 168 h of aeration, whereas a value of 90% was observed in their presence. in the early stages of the treatment, during which the redox potential passed from a value characteristic of anaerobic condit ...19863768808
[diffuse growth of strains of acinetobacter calcoaceticus on blood agar and their motility digging appearance on soft-agar medium]. 19863773266
pathogenic significance of acinetobacter calcoaceticus: analysis of experimental infection in mice.the phenomenon that mixed infection with certain species of bacteria and acinetobacter calcoaceticus is more virulent than single infection was analyzed experimentally. in mixed infections with a. calcoaceticus paired with either escherichia coli, serratia marcescens, or pseudomonas aeruginosa, the virulence of the latter three organisms was markedly increased over that of single infections only by slime-producing strains of a. calcoaceticus. of the 100 strains of a. calcoaceticus tested, 14 had ...19863773789
plasmid rp1-mediated susceptibility of acinetobacter calcoaceticus to rat polymorphonuclear leukocyte granule contents.the resistance plasmid rp1 was transferred by conjugation to a plasmidless strain of acinetobacter calcoaceticus. acquisition and expression of rp1 by a. calcoaceticus ho1-n was associated with an increase in sensitivity to the antimicrobial activity of extracted contents from rat polymorphonuclear leukocyte granules. plasmid rp1-associated antibiotic resistance and sensitivity to granule contents were cured by exposure to acridine orange. assays with granule extract fractions separated by fast ...19863781624
ultracytochemical localization of aldehyde dehydrogenase in acinetobacter calcoaceticus.a membrane-bound aldehyde dehydrogenase is induced in acinetobacter calcoaceticus grown on aliphatic hydrocarbons as sole carbon source. this enzyme is nadp-dependent and is able to oxidize medium- and long-chain aliphatic aldehydes to their corresponding fatty acids. electron micrographs of sectioned alkane-adapted bacteria showed hydrocarbon inclusions in the cytoplasmic matrix. the cytochemical phenazine methosulphate-tetranitro tetrazolium blue capture reaction allowed to localize the activi ...19863572743
alcohol oxidation by acinetobacter calcoaceticus eb 104--a n-alkane-utilizing and cytochrome p-450-producing strain.a soluble, nadp+-dependent alcohol dehydrogenase (adh) with a ph optimum at 8.7 was found in a. calcoaceticus eb 104 after growth on different carbon sources. n-alkanols with short and medium chain length were employed as test substrates. the km values decreased with increasing chain length. the vmax values remained nearly unchanged. the activities determined were independent of the carbon source. furthermore, a n-alkanol-dependent reduction of dcpip was measured in membrane fractions of cells g ...19863806370
comparative antimicrobial activity of enoxacin, ciprofloxacin, amifloxacin, norfloxacin and ofloxacin against 177 bacterial isolates.the in-vitro antimicrobial activity of five new quinolones, enoxacin (ci-919, at-2266), ciprofloxacin (bay o 9867), amifloxacin (win 49375) norfloxacin (mk 0366), and ofloxacin (orf 18489), was compared against 104 strains of enterobacteriaceae, 51 pseudomonas species, 7 acinetobacter calcoaceticus var. anitratus, and 15 enterococci. in general the quinolones tested were active against most bacterial strains. ciprofloxacin was the most active with mic90 for all genera tested ranging from 0.03 to ...19862941401
cloning and expression of acinetobacter calcoaceticus catbcde genes in pseudomonas putida and escherichia coli.this report describes the isolation and preliminary characterization of a 5.0-kilobase-pair (kbp) ecori dna restriction fragment carrying the catbcde genes from acinetobacter calcoaceticus. the respective genes encode enzymes that catalyze four consecutive reactions in the catechol branch of the beta-ketoadipate pathway: catb, muconate lactonizing enzyme (ec 5.5.1.1); catc, muconolactone isomerase (ec 5.3.3.4); catd, beta-ketoadipate enol-lactone hydrolase (ec 3.1.1.24); and cate, beta-ketoadipa ...19863003031
separation of isoenzymes of citrate synthase and isocitrate dehydrogenase by fast protein liquid chromatography.fast protein liquid chromatography (fplc) has been shown to be a rapid and effective method of separating isoenzymes of citrate synthase and isocitrate dehydrogenase in extracts of pseudomonas aeruginosa and acinetobacter calcoaceticus. the advantages of fplc over conventional methods of fractionation are discussed and it is suggested that this may be a valuable and more general technique for isoenzyme resolution.19863081366
microplate technique to determine hemolytic activity for routine typing of listeria strains.because the hemolysis produced by listeria monocytogenes and listeria seeligeri on blood agar is frequently difficult to interpret, we developed a microplate technique for the routine determination of hemolytic activity with erythrocyte suspensions. this microtechnique is a simple and reliable test for distinguishing clearly between hemolytic and nonhemolytic strains and could be used instead of the camp (christie-atkins-munch-petersen) test with staphylococcus aureus in the routine typing of li ...19863088037
in vitro activity of aztreonam against gram negative bacteria from clinical specimens and its comparison with other commonly used antibiotics.a total of 755 gram negative bacteria isolated from clinical specimens were tested against aztreonam by the disc agar diffusion test. the strains of bacteria used in this study consisted of escherichia coli (314), enterobacter aerogenes (30), e. agglomerans (7), e. cloacae, (39), citrobacter diversus (9), c. freundii (13), hafnia alvei (3), acinetobacter calcoaceticus (10), klebsiella oxytoca (6), k. ozaenae (5), k. pneumoniae (107), morganella morganii (3), moraxella sp. (10), pasteurella multo ...19863088346
enhanced emulsan production in mutants of acinetobacter calcoaceticus rag-1 selected for resistance to cetyltrimethylammonium bromide.mutants of acinetobacter calcoaceticus rag-1 that produced elevated levels of the polymeric bioemulsifier emulsan were isolated on the basis of their resistance to the cationic surfactant cetyltrimethylammonium bromide (ctab). such mutants showed maximum enhancement in both overall yield and specific productivity of some two- to threefold over that of the wild type. in addition, the effect was also observed in a resting cell system in the presence of chloramphenicol, indicating that the mutation ...19863089157
determination of endotoxins on hypodermic needles by means of a chromogenic limulus amoebocyte lysate assay. development of a test model.a test model was developed in order to describe the determination of endotoxins on hypodermic needles in a reliable and reproducible manner. as in all in-vitro experiments one has to be very careful about extrapolating data to in-vivo situations. however by choosing a hydrophilic (salmonella typhimurium atcc 14028) and a hydrophobic bacterium (acinetobacter calcoaceticus var. anitratus rr 8212/113), one could hope to obtain a quite representative idea about the extraction of contaminating gram-n ...19863101323
antibacterial properties of imipenem with special reference to the activity against methicillin-resistant staphylococci, cefotaxime-resistant enterobacteriaceae and pseudomonas aeruginosa.imipenem was examined with standardized agar dilution procedures against a wide range of bacteria. geometric mean mics against the genera escherichia, klebsiella, enterobacter, citrobacter and serratia were 0.1-0.4 mg/l, and proteus and providencia spp. were inhibited by 0.25-4 mg/l. acinetobacter calcoaceticus var. anitratum strains were inhibited by concentrations ranging from 0.12-0.5 mg/l. methicillin-susceptible staphylococci were highly susceptible to the drug (mics: less than or equal to ...19863102452
emergence of resistant bacterial strains during treatment of infections in the respiratory tract.in order to investigate the frequency of the emergence of resistance during treatment, 1,403 episodes of lower respiratory infection were studied in a general hospital with three departments of chest medicine in a period of four years. in 650 episodes the pathogen was isolated and in 82 of those failure of therapy was accompanied by emergence of resistance to the agent used. factors associated with this phenomenon were: intensive care, tracheostomy, involvement of pseudomonas aeruginosa, enterob ...19863103208
quinoprotein glucose dehydrogenase and its application in an amperometric glucose sensor.glucose dehydrogenase (gdh), one of the recently discovered nad(p)+-independent 'quinoprotein' class of oxidoreductase enzymes, was purified from acinetobacter calcoaceticus lmd 79.41 and immobilised on a 1,1'-dimethylferrocene-modified graphite foil electrode. the second-order rate constant (ks) for the transfer of electrons between gdh and ferrocenemonocarboxylic acid (fmca) in a homogeneous system, determined using direct current (dc) cyclic voltammetry, was found to be 9.4 x 10(6) litres mol ...19863454651
acinetobacter calcoaceticus sepsis in children with malignancies.the clinical and microbiologic characteristics of 29 episodes of sepsis caused by acinetobacter calcoaceticus were reviewed in 25 children with underlying malignancies. of the 29 episodes of sepsis with this organism 28 occurred from 1980 through 1984, compared with 1 episode from 1973 to 1979. risk of infection was associated with the presence of intravascular cannulae, osteosarcoma and recent administration of antitumor chemotherapy. there was no association with neutropenia, malnutrition or f ...19863463939
antimicrobial activity of imipenem against 1386 clinical isolates.1386 isolates from clinical specimens were tested against imipenem by disc agar diffusion. the bacteria used in this study consisted of escherichia coli, enterobacter aerogenes, e. agglomerans, e. cloacae, klebsiella pneumoniae, k. oxytoca, k. ozanae, proteus mirabilis, p. vulgaris, providencia stuartii, p. rettgeri, acinetobacter calcoaceticus, citrobacter diversus, c. freundii, morganella morganii, serratia liquefaciens, s. marcescens, hafnia alvei, aeromonas hydrophila, pseudomonas aeruginosa ...19863468319
susceptibility of non-fermentative gram-negative bacteria to ciprofloxacin, norfloxacin, amifloxacin, pefloxacin and cefpirome.susceptibility of 340 mainly clinically isolated gram-negative non-fermenters to ciprofloxacin, norfloxacin, amifloxacin, pefloxacin and cefpirome was determined by agar dilution. ciprofloxacin was most active, with mic90s against all organisms ranging between less than 0.125 and 4 mg/l. norfloxacin, amifloxacin and pefloxacin were active against most strains, with mic90 ranges (mg/l) of 0.5 - 32, 0.25 - 32 and less than 0.125 - 16, respectively. cefpirome showed less activity than the quinolone ...19863469178
in-vitro antibacterial activity of imipenem compared with four other beta-lactam antibiotics (ceftazidime, cefotaxime, piperacillin and azlocillin) against 828 separate clinical isolates from a portuguese hospital.an agar dilution technique was used to determine the mic50 and mic90 of imipenem in comparison with ceftazidime, cefotaxime, piperacillin and azlocillin. eight hundred and eighteen of the 828 (98.8%) unselected gram-positive cocci and gram-negative bacilli were inhibited by imipenem at a concentration of 8 mg/l or less. imipenem was shown to be superior to the other four beta-lactam antibiotics against acinetobacter calcoaceticus (40 strains), all the staphylococcus aureus (80 strains, 10 of the ...19863469195
in vitro activity of antimicrobial agents against acinetobacter calcoaceticus.the aim of this study was to evaluate the in vitro activity of several new microbial agents against 96 acinetobacter calcoaceticus isolates. among several new beta-lactams, imipenem, a new, broad-spectrum, highly potent penem, was the most active drug in vitro against these strains, with a geometric mean mic of about 0.3 mg/l. ceftazidime and ceftizoxime also demonstrated good in vitro activity with geometric mean mics of 6 mg/l and 7 mg/l respectively. among new quinolones, ciprofloxacin, oflox ...19863471431
[cell envelope-bound proteinase activities of acinetobacter calcoaceticus].acinetobacter calcoaceticus contains proteolytic activity after growth on various culture media. the enzyme activity could be found in the cytosolic fraction as well as in the cell envelopes (also containing the intracytoplasmic membranes). the highest proteolytic activity could be detected during the transition from the logarithmic growth phase to the stationary phase and in the early stationary phase, respectively. in the culture medium proteolytic activity was only evident in the later statio ...19863518708
[acinetobacter calcoaceticus strain with a wide spectrum of utilizing aromatic compounds and carrying a plasmid for resorcin degradation].a bacterial strain was isolated from soil and identified as acinetobacter calcoaceticus var. lwoffii. the strain can utilize a wide spectrum of aromatic compounds. it carries a transmissive plasmid pbsw13 which determines resorcin utilization via the ortho pathway including the following steps: resorcin-hydroxyhydroquinone-maleylacetate-beta-ketoadipi c acid. the plasmid has been transferred by conjugation into the recipient strains of a. calcoaceticus 5734 ccm rifr, escherichia coli j-53 met-pr ...19863523168
an infant with simultaneous beta-lactamase-positive haemophilus influenzae meningitis and beta-lactamase-negative h. influenzae septicemia, escherichia coli pyelonephritis and herpes encephalitis.we describe an infant with meningitis and septicemia due to infection with two different strains of haemophilus influenzae, with a urinary tract infection due to escherichia coli and in whom herpes virus encephalitis was diagnosed within three days. acinetobacter calcoaceticus septicemia developed three weeks later. no immunological deficiency could be demonstrated in the patient who recovered finally, albeit with sequelae due to encephalitis.19863524107
cloning and expression of the acinetobacter calcoaceticus mutarotase gene in escherichia coli.this article describes the cloning of the mutarotase gene from acinetobacter calcoaceticus and its expression in escherichia coli. purification of mutarotase (ec 5.1.3.3) led to a single polypeptide of 40 kilodaltons. the sequences of 27 n-terminal and 76 c-terminal amino acids were determined. from six amino acids of the n-terminal and seven amino acids of the c-terminal portion of the protein, the sequences of two oligonucleotides were deduced. these were synthesized and used as gene probes. c ...19863531172
[in vitro study of the pefloxacine-ceftriaxone combination against 18 strains of acinetobacter].the bacteriostatic and bactericidal activity of the pefloxacin-ceftriaxone combination was studied in mueller-hinton broth against 18 clinical strains of acinetobacter calcoaceticus var. anitratum by the checkerboard technique. the bactericidal activity was tested after 2, 4, 6 and 24 hours contact. mics were 0.12 to 4 mg/l for pefloxacin and 2 to 64 mg/l for ceftriaxone. the bacteriostatic interaction of the antibiotics was synergistic for 5 strains (fic index = 0.375 to 0.5) and additive for 1 ...19863534716
[comparative activity in vitro of ceftizoxime, ceftazidime and imipenem against acinetobacter calcoaceticus].acinetobacter calcoaceticus, a nosocomial pathogenic agent, is isolated with increasing frequency from hospitalized patients. acinetobacter is one of the most resistant pathogens to currently available antibiotics, particularly beta-lactam antibiotics. beta-lactamases (tem penicillinase and cephalosporinase) and problems of permeability are the most frequent mechanisms of resistance. the authors compared the in vitro activity of ceftizoxim, ceftazidim and imipenem against 82 clinical isolates of ...19863534756
cloning and expression of acinetobacter calcoaceticus catechol 1,2-dioxygenase structural gene cata in escherichia coli.catechol 1,2-dioxygenase (ec 1.13.1.1), the product of the cata gene, catalyzes the first step in catechol utilization via the beta-ketoadipate pathway. enzymes mediating subsequent steps in the pathway are encoded by the catbcde genes which are carried on a 5-kilobase-pair (kbp) ecori restriction fragment isolated from acinetobacter calcoaceticus. this dna was used as a probe to identify escherichia coli colonies carrying recombinant puc19 plasmids with overlapping sequences. repetition of the ...19863536862
role for emulsan in growth of acinetobacter calcoaceticus rag-1 on crude oil.when acinetobacter calcoaceticus rag-1 was grown together with an emulsan-deficient mutant on crude oil, only the emulsan-producing rag-1 was found to grow, regardless of whether the medium was supplemented with emulsan. the results suggested that the cell-associated form of the bioemulsifier is the biologically active species required for growth on crude oil. a revertant of an emulsan-deficient strain was isolated which simultaneously regained the ability to produce both cell-associated and cel ...198616347027
microbiological degradation of malodorous substances of swine waste under aerobic conditions.phenol, p-cresol, and volatile fatty acids (vfa; acetic, propionic, isobutyric, butyric, isovaleric, and valeric acids) were used as odor indicators of swine waste. aeration of the waste allowed the indigenous microorganisms to grow and degrade these malodorous substances. the time required for degradation of these substances varied according to the waste used, and it was not necessarily related to their concentrations. using a minimal medium which contained one of the malodorous compounds as so ...198716347254
quantification of the degree of acclimation of a mixed culture to an industrial landfill leachate.quantification of the degree of acclimation of a mixed culture is demonstrated. an industrial landfill leachate was used as a model mixed substrate. the biodegradable fraction, the specific rate of substrate uptake, and the apparent reaction order were measured. the method of acclimation did not appear to affect the biodegradable fraction. the apparent reaction order is shown to be related to the difference between the rates of uptake of the various leachate components. this difference is define ...198718581494
cloning of the genes involved in synthesis of coenzyme pyrrolo-quinoline-quinone from acinetobacter calcoaceticus.mutants of acinetobacter calcoaceticus lmd79.41 were isolated that are defective in the synthesis of the coenzyme pyrrolo-quinoline-quinone (pqq). a gene bank of the wild-type. a. calcoaceticus genome was constructed with the binary plasmid system plv21-rp4 delta km. the dna of a. calcoaceticus lmd79.41 was partially digested with sau3a, and fragments of about 15 kilobases were inserted into the bamhi site of plv21. the hybrid plasmids maintained in escherichia coli were transferred by conjugati ...19873539922
influence of the catbce sequence on the phenotypic reversion of a pcae mutation in acinetobacter calcoaceticus.isofunctional beta-ketoadipate:succinyl coenzyme a transferases i and ii are encoded by the pcae and cate genes, respectively, of acinetobacter calcoaceticus. the genes are under separate transcriptional control and genetically unlinked. mutations in the pcae gene result in a p-hydroxybenzoate-negative (pob-) phenotype, whereas cate mutations cause a benzoate-negative (ben-) phenotype. a. calcoaceticus adp125 carries the pcae3125 mutation and gave rise to pob+ revertants with a frequency of 10(- ...19873474227
in vitro and in vivo activity of ny-198, a new difluorinated quinolone.ny-198 [1-ethyl-6,8-difluoro-1,4-dihydro-7-(3-methyl-1-piperazinyl)-4-oxo-3- quinolinecarboxylic acid hydrochloride] is a new difluorinated quinolone characterized by the presence of a c-methyl group at the 3 position of the piperazine moiety. it has a broad antibacterial spectrum. the in vitro antibacterial activity of ny-198 was almost the same as those of ofloxacin and norfloxacin, but far exceeded that of pipemidic acid. ny-198 was more active than norfloxacin against pseudomonas maltophilia ...19873476021
hospital acquired native valve endocarditis caused by acinetobacter calcoaceticus and treated with imipenem/cilastin.a case of hospital acquired endocarditis due to acinetobacter calcoaceticus in a severely burned patient is presented. both aortic and mitral native valves were affected and the organism was resistant to most antimicrobial agents.19873479562
isolation and characterization of a prokaryotic sulfurtransferase.a sulfurtransferase has been purified to apparent homogeneity from the prokaryote acinetobacter calcoaceticus lwoffi by conventional protein fractionation techniques. steady-state kinetic studies of the enzyme revealed that its formal mechanism varies with the acceptor substrate employed. with inorganic thiosulfate as the sulfane sulfur-donor substrate and cyanide anion as the acceptor, the enzyme was shown to catalyze the reaction by a double displacement mechanism like that of mammalian rhodan ...19873480285
leucine aminopeptidase in intracytoplasmic membranes of acinetobacter calcoaceticus.cells of acinetobacter calcoaceticus strain 69-v contain an aminopeptidase that cleaves l-leucine amide, leucylglycine or leucine hydrazide with high efficiency. leucine 4-nitroanilide and hydrazide are hydrolyzed to less than 0.1% and 1%, resp. of leucine amide. grown on acetate-nh4+ medium the activity of the enzyme in the cytoplasm is increased 5-fold compared with cells grown on a casamino acid medium or on yeast extract. in these cases the specific activity of the unpurified enzyme is about ...19873503925
[selection of mutants of microorganisms utilizing ethanol].mutants of the bacteria acinetobacter calcoaceticus 34 and acinetobacter sp. 172 as well as of the yeast candida requinyii 316 resistant to acetaldehyde grow better in a medium with ethanol than their parent cultures. in their specific growth rate and alcohol dehydrogenase activity, 28.7-66.7% of such mutants are superior to any clone isolated in a non-selective medium. a medium containing ethanol and acetaldehyde (0.5 to 1.0% by volume) is proposed to select and isolate highly productive mutant ...19873295493
detection of the cofactor pyrroloquinoline quinone.in order to demonstrate the presence or absence of a pyrroloquinoline quinone (pqq) synthesizing capacity in microorganisms, we have found that media and equipment must be treated to remove contaminating pqq. procedures are described which appear to be effective for that purpose. these have been used with acinetobacter calcoaceticus pqq- strains to develop a sensitive and reliable assay for pqq. they also have been used to show that under our conditions of growth escherichia coli does not synthe ...19873300411
[evaluation of the atb system and the 32 gn tests for the identification and determination of the antibiotic sensitivity of acinetobacter calcoaceticus var. anitratum. comparison with the reference dilution method in a gel medium].the authors determined the susceptibility to 24 antibiotics of 95 strains of acinetobacter calcoaceticus variety anitratum with the automated api atb system in comparison with the agar dilution method for mic measurement. the strains have been identified with api 32 gn (automated system) and api ne. there was a good agreement between the two sets of identification results. in susceptibility tests the discrepancies ranged from 0.86% to 3% with a mean value of 1.8%. the overall agreement was 95% b ...19873302861
[proteases in different membrane fractions of acinetobacter calcoaceticus].distinct protease activities were found in membrane fractions from acinetobacter calcoaceticus grown on acetate-nh4+ medium until early stationary phase. mechanical or enzymatic cell disintegration followed by membrane fractionation through sucrose gradient revealed higher activities in the outer membrane than in the cytoplasmic membrane. using azocasein and synthetic p-nitroanilides as substrates we found very low proteinase activities in intracytoplasmic membrane fractions. however, these frac ...19873309254
purification and properties of l-mandelate dehydrogenase and comparison with other membrane-bound dehydrogenases from acinetobacter calcoaceticus.l-mandelate dehydrogenase was purified from acinetobacter calcoaceticus by triton x-100 extraction from a 'wall + membrane' fraction, ion-exchange chromatography on deae-sephacel, (nh4)2so4 fractionation and gel filtration followed by further ion-exchange chromatography. the purified enzyme was partially characterized with respect to its subunit mr (44,000), ph optimum (7.5), pi value (4.2), substrate specificity and susceptibility to various potential inhibitors including thiol-blocking reagent ...19873325042
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