Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| alkaline cyanide degradation by pseudomonas pseudoalcaligenes cect5344 in a batch reactor. influence of ph. | water containing cyanide was biologically detoxified with the bacterial strain pseudomonas pseudoalcaligenes cect5344 in a batch reactor. volatilization of toxic hydrogen cyanide (hcn) was avoided by using an alkaline medium for the treatment. the operational procedure was optimized to assess cyanide biodegradation at variable ph values and dissolved oxygen concentrations. using an initial ph of 10 without subsequent adjustment allowed total cyanide to be consumed at a mean rate of approximately ... | 2010 | 20346583 |
| experimental evidence that source genetic variation drives pathogen emergence. | a pathogen can readily mutate to infect new host types, but this does not guarantee successful establishment in the new habitat. what factors, then, dictate emergence success? one possibility is that the pathogen population cannot sustain itself on the new host type (i.e. host is a sink), but migration from a source population allows adaptive sustainability and eventual emergence by delivering beneficial mutations sampled from the source's standing genetic variation. this idea is relevant regard ... | 2010 | 20484240 |
| nitroaromatic compounds, from synthesis to biodegradation. | nitroaromatic compounds are relatively rare in nature and have been introduced into the environment mainly by human activities. this important class of industrial chemicals is widely used in the synthesis of many diverse products, including dyes, polymers, pesticides, and explosives. unfortunately, their extensive use has led to environmental contamination of soil and groundwater. the nitro group, which provides chemical and functional diversity in these molecules, also contributes to the recalc ... | 2010 | 20508249 |
| an intramolecular disulfide bond is required for the thermostability of methyl parathion hydrolase, ophc2. | ophc2, a methyl parathion hydrolase (mph) from pseudomonas pseudoalcaligenes c2-1 (cgmcc 1150), can degrade a wide range of organophosphate pesticides. compared with other mphs, ophc2 exhibits high thermostability. its thermostability mechanism, however, remains unknown. in the present study, sequence analysis demonstrated that two cysteines (cys110 and cys146) exist in ophc2, but not in other mphs. the three-dimensional structural model of ophc2 performed by computer-assisted homology modelling ... | 2010 | 20607231 |
| structural characterization of iscr8, iscr22, and iscr23, subgroups of is91-like insertion elements. | analysis of iscr8 (ispps1) revealed that this group of insertion elements has to be subdivided into three subgroups: iscr8, iscr22, and iscr23. the distinction of three subgroups is supported by phylogenetic analysis of the transposase open reading frames (orfs). comparison of over 20 complete and partial iscr8/22/23 elements identified oriis candidate sequences for all groups and a teris candidate sequence for iscr8. the oriis sequences, their distance to the transposase orfs, and the sequence ... | 2010 | 20625149 |
| efficiency of phenol biodegradation by planktonic pseudomonas pseudoalcaligenes (a constructed wetland isolate) vs. root and gravel biofilm. | in the last two decades, constructed wetland systems gained increasing interest in wastewater treatment and as such have been intensively studied around the world. while most of the studies showed excellent removal of various pollutants, the exact contribution, in kinetic terms, of its particular components (such as: root, gravel and water) combined with bacteria is almost nonexistent. in the present study, a phenol degrader bacterium identified as pseudomonas pseudoalcaligenes was isolated from ... | 2010 | 20705318 |
| hybrid pseudomonads engineered by two-step homologous recombination acquire novel degradation abilities toward aromatics and polychlorinated biphenyls. | pseudomonas pseudoalcaligenes kf707 possesses a chromosomally encoded bph gene cluster responsible for the catabolism of biphenyl and polychlorinated biphenyls. previously, we constructed chimeric versions of the bpha1 gene, which encodes a large subunit of biphenyl dioxygenase, by using dna shuffling between bpha1 genes from p. pseudoalcaligenes kf707 and burkholderia xenovorans lb400. in this study, we demonstrate replacement of the bpha1 gene with chimeric bpha1 sequence within the chromosoma ... | 2010 | 20809076 |
| biodegradation of polyfluorinated biphenyl in bacteria. | fluorinated aromatic compounds are significant environmental pollutants, and microorganisms play important roles in their biodegradation. the effect of fluorine substitution on the transformation of fluorobiphenyl in two bacteria was investigated. pseudomonas pseudoalcaligenes kf707 and burkholderia xenovorans lb400 used 2,3,4,5,6-pentafluorobiphenyl and 4,4'-difluorobiphenyl as sole sources of carbon and energy. the catabolism of the fluorinated compounds was examined by gas chromatography-mass ... | 2010 | 20830605 |
| tolerance of pseudomonas pseudoalcaligenes kf707 to metals, polychlorobiphenyls and chlorobenzoates: effects on chemotaxis-, biofilm- and planktonic-grown cells. | pseudomonas pseudoalcaligenes kf707 is a polychlorinated biphenyls (pcbs) degrader, also tolerant to several toxic metals and metalloids. the work presented here examines for the first time the chemotactic response of p. pseudoalcaligenes kf707 to biphenyl and intermediates of the pcb biodegradation pathway in the presence and absence of metals. chemotaxis analyses showed that biphenyl, benzoic acid and chlorobenzoic acids acted as chemoattractants for kf707 cells and that metal cations such as ... | 2010 | 20846140 |
| application of a novel bacterial consortium for mineralization of sulphonated aromatic amines. | a novel bacterial consortium (tj-2) for mineralization of aromatic amines resulting from decolorization of azo dyes was developed. three bacterial strains were identified as pseudomonas pseudoalcaligenes (tj-21,eu072476), pseudomonas citronellolis (tj-22,eu072477) and pseudomonas testosterone (tj-23,eu072477) by 16s rrna gene sequence analysis. aromatic amine mineralization under aerobic conditions was observed to be significantly higher with the consortium as compared to pure strains indicating ... | 2010 | 20863689 |
| absolute configuration-dependent epoxide formation from isoflavan-4-ol stereoisomers by biphenyl dioxygenase of pseudomonas pseudoalcaligenes strain kf707. | biphenyl dioxygenase from pseudomonas pseudoalcaligenes strain kf707 expressed in escherichia coli was found to exhibit monooxygenase activity toward four stereoisomers of isoflavan-4-ol. lc-ms and lc-nmr analyses of the metabolites revealed that the corresponding epoxides formed between c2' and c3' on the b-ring of each isoflavan-4-ol substrate were the sole products. the relative reactivity of the stereoisomers was found to be in the order: (3s,4s)-cis-isoflavan-4-ol > (3r,4s)-trans-isoflavan- ... | 2010 | 21063701 |
| structural insight into the expanded pcb-degrading abilities of a biphenyl dioxygenase obtained by directed evolution. | the biphenyl dioxygenase of burkholderia xenovorans lb400 is a multicomponent rieske-type oxygenase that catalyzes the dihydroxylation of biphenyl and many polychlorinated biphenyls (pcbs). the structural bases for the substrate specificity of the enzyme's oxygenase component (bphae(lb400)) are largely unknown. bphae(p4), a variant previously obtained through directed evolution, transforms several chlorobiphenyls, including 2,6-dichlorobiphenyl, more efficiently than bphae(lb400), yet differs fr ... | 2010 | 21073881 |
| structural insight into the expanded pcb-degrading abilities of a biphenyl dioxygenase obtained by directed evolution. | the biphenyl dioxygenase of burkholderia xenovorans lb400 is a multicomponent rieske-type oxygenase that catalyzes the dihydroxylation of biphenyl and many polychlorinated biphenyls (pcbs). the structural bases for the substrate specificity of the enzyme's oxygenase component (bphae(lb400)) are largely unknown. bphae(p4), a variant previously obtained through directed evolution, transforms several chlorobiphenyls, including 2,6-dichlorobiphenyl, more efficiently than bphae(lb400), yet differs fr ... | 2010 | 21073881 |
| metalworking fluids biodiversity characterization. | hypersensitivity pneumonitis of machinists associated with metalworking fluids (mwf) was recently linked to mycobacterium immunogenum. in addition to mycobacterium, impacts of continuous and massive contact to other micro-organisms, such as pseudomonas, were little studied. this report intended to quantify and characterize the microbial load of 44 in-use mwf. | 2010 | 19614850 |
| diversity of clavulanic acid-inhibited extended-spectrum β-lactamases in aeromonas spp. from the seine river, paris, france. | environmental aeromonas sp. isolates resistant to ceftazidime were recovered during an environmental survey performed with water samples from the seine river, in paris, france, in november 2009. selected isolates were identified by sequencing of the 16s rrna and rpob genes. pcr and cloning experiments were used to identify broad-spectrum-β-lactamase-encoding genes and their genetic context. clavulanic acid-inhibited extended-spectrum-β-lactamase (esbl) genes were identified in 71% of the aeromon ... | 2010 | 21149627 |
| diversity of clavulanic acid-inhibited extended-spectrum β-lactamases in aeromonas spp. from the seine river, paris, france. | environmental aeromonas sp. isolates resistant to ceftazidime were recovered during an environmental survey performed with water samples from the seine river, in paris, france, in november 2009. selected isolates were identified by sequencing of the 16s rrna and rpob genes. pcr and cloning experiments were used to identify broad-spectrum-β-lactamase-encoding genes and their genetic context. clavulanic acid-inhibited extended-spectrum-β-lactamase (esbl) genes were identified in 71% of the aeromon ... | 2010 | 21149627 |
| cyanide degradation by pseudomonas pseudoalcaligenes cect5344 involves a malate:quinone oxidoreductase and an associated cyanide-insensitive electron transfer chain. | the alkaliphilic bacterium pseudomonas pseudoalcaligenes cect5344 is able to grow with cyanide as the sole nitrogen source. membrane fractions from cells grown under cyanotrophic conditions catalysed the production of oxaloacetate from l-malate. several enzymic activities of the tricarboxylic acid and glyoxylate cycles in association with the cyanide-insensitive respiratory pathway seem to be responsible for the oxaloacetate formation in vivo. thus, in cyanide-grown cells, citrate synthase and i ... | 2010 | 21178163 |
| expression in escherichia coli of biphenyl 2,3-dioxygenase genes from a gram-positive polychlorinated biphenyl degrader, rhodococcus jostii rha1. | rhodococcus jostii rha1 is a polychlorinated biphenyl degrader. multi-component biphenyl 2,3-dioxygenase (bpha) genes of rha1 encode large and small subunits of oxygenase component and ferredoxin and reductase components. they did not express enzyme activity in escherichia coli. to obtain bpha activity in e. coli, hybrid bpha gene derivatives were constructed by replacing ferredoxin and/or reductase component genes of rha1 with those of pseudomonas pseudoalcaligenes kf707. the results obtained i ... | 2011 | 21228494 |
| the escherichia coli btue protein functions as a resistance determinant against reactive oxygen species. | this work shows that the recently described escherichia coli btue peroxidase protects the bacterium against oxidative stress that is generated by tellurite and by other reactive oxygen species elicitors (ros). cells lacking btue (?btue) displayed higher sensitivity to k(2)teo(3) and other oxidative stress-generating agents than did the isogenic, parental, wild-type strain. they also exhibited increased levels of cytoplasmic reactive oxygen species, oxidized proteins, thiobarbituric acid reactive ... | 2011 | 21264338 |
| bacterial cyanide degradation is under review: pseudomonas pseudoalcaligenes cect5344, a case of an alkaliphilic cyanotroph. | there are thousands of areas in the u.s.a. and europe contaminated with cyanide-containing wastes as a consequence of a large number of industrial activities such as gold mining, steel and aluminium manufacturing, electroplating and nitrile pesticides used in agriculture. chemical treatments to remove cyanide are expensive and generate other toxic products. by contrast, cyanide biodegradation constitutes an appropriate alternative treatment. in the present review we provide an overview of how ce ... | 2011 | 21265786 |
| rapid identification of gram-negative bacteria with and without ctx-m extended-spectrum ß-lactamase from positive blood culture bottles by pcr followed by microchip gel electrophoresis. | we evaluated the usefulness of pcr analysis of the 16s-23s rrna gene internal transcribed spacer (its) and the ctx-m extended-spectrum ß-lactamase (esbl) followed by microchip gel electrophoresis (mge) for direct identification and ctx-m detection of gram-negative bacteria (gnb) from positive blood culture bottles. of 251 gnb isolated from blood cultures containing a single bacterium, 225 (90%) were correctly identified at the species level directly from positive blood culture bottles by compari ... | 2011 | 21289149 |
| development of a strain for efficient degradation of polychlorinated biphenyls by patchwork assembly of degradation pathways. | rhodococcus jostii rha1 accumulates chlorobenzoates (cba) during the degradation of polychlorinated biphenyls (pcbs). cba degradation is considered one of the rate-limiting steps in the complete degradation of pcbs. to reduce the accumulation of cbas, the upper pathway enzyme genes for pcb degradation of rha1 were introduced into a cba-degrading bacterium, burkholderia sp. nk8. the resulting recombinant strain exhibited no biphenyl 2,3-dioxygenase (bpha) activity encoded by bphaaabacad genes, wh ... | 2011 | 21310654 |
| extracellular streptomyces vesicles: amphorae for survival and defence. | blue-pigmented exudates arise as droplets on sporulated lawns of streptomyces coelicolor m110 grown on agar plates. our electron microscopical and biochemical studies suggest that droplets contain densely packed vesicles with large assemblies of different protein types and/or the polyketide antibiotic actinorhodin. frozen-hydrated vesicles were unilamellar with a typical bilayer membrane, and ranged from 80 to 400 nm in diameter with a preferred width of 150-300 nm. by means of cryo-electron tom ... | 2011 | 21342473 |
| automated identification of medically important bacteria by 16s rrna gene sequencing using a novel comprehensive database, 16spathdb. | despite the increasing use of 16s rrna gene sequencing, interpretation of 16s rrna gene sequence results is one of the most difficult problems faced by clinical microbiologists and technicians. to overcome the problems we encountered in the existing databases during 16s rrna gene sequence interpretation, we built a comprehensive database, 16spathdb (http://147.8.74.24/16spathdb) based on the 16s rrna gene sequences of all medically important bacteria listed in the manual of clinical microbiology ... | 2011 | 21389154 |
| comparative activities of tigecycline and other tetracyclines against non-fermenting gram-negative bacilli (excluding acinetobacter spp.). | tigecycline, a glycylcycline, is a semisynthetic derivative of minocycline with a broad spectrum of activity against aerobic and anaerobic bacteria (2, 12, 16).… | 2011 | 21606223 |
| flavonoids biotransformation by bacterial non-heme dioxygenases, biphenyl and naphthalene dioxygenase. | this review details recent progresses in the flavonoid biotransformation by bacterial non-heme dioxygenases, biphenyl dioxygenase (bdo), and naphthalene dioxygenase (ndo), which can initially activate biphenyl and naphthalene with insertion of dioxygen in stereospecfic and regiospecific manners. flavone, isoflavone, flavanone, and isoflavanol were biotransformed by bdo from pseudomonas pseudoalcaligenes kf707 and ndo from pseudomonas sp. strain ncib9816-4, respectively. in general, bdo showed wi ... | 2011 | 21626021 |
| xenopus laevis oocytes infected with multi-drug-resistant bacteria: implications for electrical recordings. | the xenopus laevis oocyte has been the workhorse for the investigation of ion transport proteins. these large cells have spawned a multitude of novel techniques that are unfathomable in mammalian cells, yet the fickleness of the oocyte has driven many researchers to use other membrane protein expression systems. here, we show that some colonies of xenopus laevis are infected with three multi-drug-resistant bacteria: pseudomonas fluorescens, pseudomonas putida, and stenotrophomonas maltophilia. o ... | 2011 | 21788613 |
| improving the thermostability of a methyl parathion hydrolase by adding the ionic bond on protein surface. | the thermostability of the methyl parathion hydrolase (mph_och) from ochrobactrum sp. m231 was improved using site-directed mutagenesis. two prolines (pro76 and pro78) located on the protein surface were selected for mutations after inspection of the sequence alignment of mph_och and ophc2, a thermostable organophosphorus hydrolase from pseudomonas pseudoalcaligenes c2-1. the temperature of the double-point mutant (p76d/p78k) at which the mutant lost 50% of its activity (t50) was approximately 6 ... | 2011 | 21728027 |
| Role of Fur on cyanide tolerance of Pseudomonas pseudoalcaligenes CECT5344. | Pseudomonas pseudoalcaligenes CECT5344 can be used in cyanide bioremediation processes because it grows at pH 9.5 using 2.0 mM cyanide at the sole nitrogen source. Cyanide strongly binds to metals creating iron-deprivation conditions. The bacterium responds to the presence of cyanide by inducing several processes such as siderophore synthesis for iron capture, cyanide-insensitive respiration system and defence mechanisms against oxidative stress. Since high concentrations of cyanide cause iron d ... | 2011 | 22103539 |
| Spoilage potentials and antimicrobial resistance of Pseudomonas spp. isolated from cheeses. | Pseudomonas spp. are aerobic, gram-negative bacteria that are recognized as major food spoilage microorganisms. A total of 32 (22.9%) Pseudomonas spp. from 140 homemade white cheese samples collected from the open-air public bazaar were isolated and characterized. The aim of the present study was to investigate the biochemical characteristics, the production of extracellular enzymes, slime and ß-lactamase, and antimicrobial susceptibility of Pseudomonas spp. isolated from cheeses. The identified ... | 2011 | 22118075 |
| the conserved yjgf protein family deaminates enamine/imine intermediates of pyridoxal-5'-phosphate (plp)-dependent enzyme reactions. | the yjgf/yer057c/uk114 family of proteins is conserved in all domains of life, suggesting that the role of these proteins arose early and was maintained throughout evolution. metabolic consequences of lacking this protein in salmonella enterica and other organisms have been described, but the biochemical function of yjgf remained unknown. this work provides the first description of a conserved biochemical activity for the yjgf protein family. our data support the conclusion that yjgf proteins ha ... | 2011 | 22094463 |
| the conserved yjgf protein family deaminates enamine/imine intermediates of pyridoxal-5'-phosphate (plp)-dependent enzyme reactions. | the yjgf/yer057c/uk114 family of proteins is conserved in all domains of life, suggesting that the role of these proteins arose early and was maintained throughout evolution. metabolic consequences of lacking this protein in salmonella enterica and other organisms have been described, but the biochemical function of yjgf remained unknown. this work provides the first description of a conserved biochemical activity for the yjgf protein family. our data support the conclusion that yjgf proteins ha ... | 2011 | 22094463 |
| biochemical studies and ligand-bound structures of biphenyl dehydrogenase from pandoraea pnomenusa strain b-356 reveal a basis for broad specificity of the enzyme. | biphenyl dehydrogenase, a member of short-chain dehydrogenase/reductase enzymes, catalyzes the second step of the biphenyl/polychlorinated biphenyls catabolic pathway in bacteria. to understand the molecular basis for the broad substrate specificity of pandoraea pnomenusa strain b-356 biphenyl dehydrogenase (bphb(b-356)), the crystal structures of the apo-enzyme, the binary complex with nad(+), and the ternary complexes with nad(+)-2,3-dihydroxybiphenyl and nad(+)-4,4'-dihydroxybiphenyl were det ... | 2011 | 21880718 |
| purification and characterization of hydroquinone dioxygenase from sphingomonas sp. strain ttnp3. | abstract: hydroquinone-1,2-dioxygenase, an enzyme involved in the degradation of alkylphenols in sphingomonas sp. strain ttnp3 was purified to apparent homogeneity. the extradiol dioxygenase catalyzed the ring fission of hydroquinone to 4-hydroxymuconic semialdehyde and the degradation of chlorinated and several alkylated hydroquinones. the activity of 1 mg of the purified enzyme with unsubstituted hydroquinone was 6.1 μmol per minute, the apparent km 2.2 μm. icp-ms analysis revealed an iron con ... | 2011 | 21906340 |
| carbapenem-hydrolyzing ges-5-encoding gene on different plasmid types from a sewage treatment plant. | plasmids prsb113 and prsb115 were recovered from an activated sludge bacterial community of a municipal wastewater treatment plant in germany. both plasmids carry the same bla(ges-5) carbapenemase gene that is located within two distinct class 1 integrons. these plasmids have different backbones, belong to different incompatibility groups and could replicate in both pseudomonas aeruginosa and escherichia coli. | 2011 | 22156421 |
| impact of an indigenous microbial enhanced oil recovery field trial on microbial community structure in a high pour-point oil reservoir. | based on preliminary investigation of microbial populations in a high pour-point oil reservoir, an indigenous microbial enhanced oil recovery (meor) field trial was carried out. the purpose of the study is to reveal the impact of the indigenous meor process on microbial community structure in the oil reservoir using 16sr dna clone library technique. the detailed monitoring results showed significant response of microbial communities during the field trial and large discrepancies of stimulated mi ... | 2011 | 22159733 |
| Metabolic adaptation of Pseudomonas pseudoalcaligenes CECT5344 to cyanide: role of malate-quinone oxidoreductases, aconitase and fumarase isoenzymes. | In general, the biodegradation of a toxic compound by a micro-organism requires the concurrence of, at least, two features in the biological system: first, the capability of the micro-organism to metabolize the toxic compound, and secondly, the capacity to resist its toxic effect. Pseudomonas pseudoalcaligenes CECT5344 is a bacterium used in the biodegradation of cyanide because it is capable to use it as a nitrogen source. The present review is mainly focused on the putative role of iron-contai ... | 2011 | 22103538 |
| strategy for improving extracellular lipolytic activities by a novel thermotolerant staphylococcus sp. strain. | extracellular bacterial lipases received much attention for their substrate specificity and their ability to function under extreme environments (ph, temperature...). many staphylococci produced lipases which were released into the culture medium. reports of extracellular thermostable lipases from staphylococcus sp. and active in alkaline conditions are not previously described. | 2011 | 22078466 |
| Production of a solvent, detergent, and thermotolerant lipase by a newly isolated Acinetobacter sp. in submerged and solid-state fermentations. | The lipase production ability of a newly isolated Acinetobacter sp. in submerged (SmF) and solid-state (SSF) fermentations was evaluated. The results demonstrated this strain as one of the rare bacterium, which is able to grow and produce lipase in SSF even more than SmF. Coconut oil cake as a cheap agroindustrial residue was employed as the solid substrate. The lipase production was optimized in both media using artificial neural network. Multilayer normal and full feed forward backpropagation ... | 2011 | 21960739 |
| Cutinolytic esterase activity of bacteria isolated from mixed-plant compost and characterization of a cutinase gene from Pseudomonas pseudoalcaligenes. | The objective of the current study was to examine cutinolytic esterase (i.e., cutinase) activity by pseudomonads and bacteria isolated from mixed-plant compost. Approximately 400 isolates representing 52 taxa recovered from mixed-plant compost using cuticle baits, along with 117 pseudomonad isolates obtained from a culture collection (i.e., non-compost habitats), were evaluated. The ability of isolates to degrade the synthetic cutin polycaprolactone (PCL) was initially measured. Isolates from 23 ... | 2011 | 22029433 |
| a histidine-kinase chea gene of pseudomonas pseudoalcaligens kf707 not only has a key role in chemotaxis but also affects biofilm formation and cell metabolism. | a histidine-kinase chea gene in pseudomonas pseudoalcaligenes kf707 plays a central role in the regulation of metabolic responses as well as in chemotaxis. non-chemotactic mutants harboring insertions into the chea gene were screened for their ability to form biofilms in the calgary biofilm device. notably, ≥95% decrease in the number of cells attached to the polystyrene surface was observed in chea mutants compared to the kf707 wild-type biofilm phenotype. the ability to form mature biofilms wa ... | 2011 | 21108067 |
| proteomics analysis of the effects of cyanate on chromobacterium violaceum metabolism. | chromobacterium violaceum is a gram-negative betaproteobacterium that has been isolated from various brazilian ecosystems. its genome contains the cyn operon, which gives it the ability to metabolize highly toxic cyanate into ammonium and carbon dioxide. we used a proteomics approach to investigate the effects of cyanate on the metabolism of this bacterium. the proteome of cells grown with and without cyanate was compared on 2-d gels. differential spots were digested and identified by mass spect ... | 2011 | 24710289 |
| plasmid metagenome reveals high levels of antibiotic resistance genes and mobile genetic elements in activated sludge. | the overuse or misuse of antibiotics has accelerated antibiotic resistance, creating a major challenge for the public health in the world. sewage treatment plants (stps) are considered as important reservoirs for antibiotic resistance genes (args) and activated sludge characterized with high microbial density and diversity facilitates arg horizontal gene transfer (hgt) via mobile genetic elements (mges). however, little is known regarding the pool of args and mges in sludge microbiome. in this s ... | 2011 | 22016806 |
| occurrence of plasmids in the aromatic degrading bacterioplankton of the baltic sea. | plasmids are mobile genetic elements that provide their hosts with many beneficial traits including in some cases the ability to degrade different aromatic compounds. to fulfill the knowledge gap regarding catabolic plasmids of the baltic sea water, a total of 209 biodegrading bacterial strains were isolated and screened for the presence of these mobile genetic elements. we found that both large and small plasmids are common in the cultivable baltic sea bacterioplankton and are particularly prev ... | 2011 | 24710296 |
| antioxidant, antimicrobial activity and total phenolic content within the aerial parts of artemisia absinthum, artemisia santonicum and saponaria officinalis. | three native turkish medicinal and aromatic plants (artemisia absinthum, artemisia santonicum and saponaria officinalis) were investigated to analyze their antioxidant activity, total phenolic content and antimicrobial activity. their total antioxidant activity was determined by using a β-carotene bleaching assay and their antimicrobial activity was determined by utilizing an agar disc diffusion assay. methanol extracts of the three species analyzed showed high antioxidant activity and among the ... | 2011 | 24363680 |
| evaluation of the bruker biotyper and vitek ms matrix-assisted laser desorption ionization-time of flight mass spectrometry systems for identification of nonfermenting gram-negative bacilli isolated from cultures from cystic fibrosis patients. | the bruker biotyper and vitek ms matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry (ms) instruments were evaluated for the identification of nonfermenting gram-negative bacilli (nfgnb) by a blinded comparison to conventional biochemical or molecular methods. two hundred nfgnb that were recovered from cultures from cystic fibrosis patients in the university of iowa health care (uihc) microbiology laboratory between 1 january 2006 and 31 october 2010 were sen ... | 2012 | 22495566 |
| multidrug-resistance and toxic metal tolerance of medically important bacteria isolated from an aquaculture system. | the use of antimicrobials and toxic metals should be considered carefully in aquaculture and surrounding environments. we aimed to evaluate medically relevant bacteria in an aquaculture system and their susceptibility to antimicrobials and toxic metals. selective cultures for enterobacteria (ent), non-fermenting gram-negative rods (nfr) and gram-positive cocci (gpc) were obtained from water samples collected in two different year seasons. the isolated bacteria were biochemically identified and a ... | 2012 | 22972388 |
| bactquant: an enhanced broad-coverage bacterial quantitative real-time pcr assay. | bacterial load quantification is a critical component of bacterial community analysis, but a culture-independent method capable of detecting and quantifying diverse bacteria is needed. based on our analysis of a diverse collection of 16 s rrna gene sequences, we designed a broad-coverage quantitative real-time pcr (qpcr) assay--bactquant--for quantifying 16 s rrna gene copy number and estimating bacterial load. we further utilized in silico evaluation to complement laboratory-based qpcr characte ... | 2012 | 22510143 |
| metagenomic analysis of subgingival microbiota following non-surgical periodontal therapy: a pilot study. | this study tested the feasibility of a high throughput metagenomic approach to analyze the pre- and posttreatment of subgingival plaque in two subjects with aggressive periodontitis. dna was extracted from subgingival samples and subjected to pcr amplification of the c2-c4 regions of the 16s rdna using primers with bar codes to identify individual samples. the pcr products were pooled and sequenced for the v4 region of the 16s rdna using the 454 flx standard platform. the results were analyzed f ... | 2012 | 23341849 |
| bacterial communities and the nitrogen cycle in the gypsum soils of cuatro ciénegas basin, coahuila: a mars analogue. | the omega/mars express hyperspectral imager identified gypsum at several sites on mars in 2005. these minerals constitute a direct record of past aqueous activity and are important with regard to the search of extraterrestrial life. gale crater was chosen as mars science laboratory curiosity's landing site because it is rich in gypsum, as are some desert soils of the cuatro ciénegas basin (ccb) (chihuahuan desert, mexico). the gypsum of the ccb, which is overlain by minimal carbonate deposits, w ... | 2012 | 22920518 |
| a randomized trial to evaluate a launderable bed protection system for hospital beds. | hospital beds are potential reservoirs of bacteria in hospitals. preventing contamination of the bed and providing a cleaner surface should help prevent hospital-acquired infections (hais). most hospital beds are cleaned between patients (terminal cleaning) using quaternary ammonia compounds (quats). | 2012 | 22958605 |
| crystallization and preliminary x-ray diffraction analysis of the organophosphorus hydrolase ophc2 from pseudomonas pseudoalcaligenes. | enzymes that are capable of degrading neurotoxic organophosphorus compounds are of increasing interest because of the lack of efficient and clean methods for their removal. recently, a novel organophosphorus hydrolase belonging to the metallo-β-lactamase superfamily was identified and isolated from the mesophilic bacterium pseudomonas pseudoalcaligenes. this enzyme, named ophc2, is endowed with significant thermal and ph stability, making it an appealing candidate for engineering studies to deve ... | 2012 | 23295492 |
| crystallization and preliminary x-ray diffraction analysis of the organophosphorus hydrolase ophc2 from pseudomonas pseudoalcaligenes. | enzymes that are capable of degrading neurotoxic organophosphorus compounds are of increasing interest because of the lack of efficient and clean methods for their removal. recently, a novel organophosphorus hydrolase belonging to the metallo-β-lactamase superfamily was identified and isolated from the mesophilic bacterium pseudomonas pseudoalcaligenes. this enzyme, named ophc2, is endowed with significant thermal and ph stability, making it an appealing candidate for engineering studies to deve ... | 2012 | 23295492 |
| first report of an extensively drug-resistant vim-2 metallo-β-lactamase-producing brevundimonas diminuta clinical isolate. | in the literature, only three brevundimonas diminuta environmental isolates carrying metallo-β-lactamase genes were recently published. however, so far, no b. diminuta clinical isolates carrying these carbapenem resistance genes have been described. here we report the first vim-2 metallo-β-lactamase-producing b. diminuta clinical isolate obtained from an immunocompromised patient. | 2012 | 22692741 |
| origin and evolution of antibiotic resistance: the common mechanisms of emergence and spread in water bodies. | the environment, and especially freshwater, constitutes a reactor where the evolution and the rise of new resistances occur. in water bodies such as waste water effluents, lakes, and rivers or streams, bacteria from different sources, e.g., urban, industrial, and agricultural waste, probably selected by intensive antibiotic usage, are collected and mixed with environmental species. this may cause two effects on the development of antibiotic resistances: first, the contamination of water by antib ... | 2012 | 22303296 |
| genetic diversity and ecological evaluation of fluorescent pseudomonads isolated from the leaves and roots of potato plants. | a total of 828 isolates of fluorescent pseudomonads (fps) were obtained from the leaves (305 isolates) and roots (523 isolates) of potato plants grown in different geographical locations in japan, and 16s rrna gene sequences of 776 isolates were successfully determined by direct pcr sequencing. clustering analysis (≥99% identity) identified 13 and 26 operational taxonomic units (otus) for leaf- and root-associated fps, respectively, and 29 otus were identified in the phytosphere of potato plants ... | 2012 | 22791043 |
| possible reasons for past failures of genetic engineering techniques for creating novel, xenobiotics-degrading bacteria. | 2012 | 22705891 | |
| degradation of 3-phenoxybenzoic acid by a bacillus sp. | 3-phenoxybenzoic acid (3-pba) is of great environmental concern with regards to endocrine disrupting activity and widespread occurrence in water and soil, yet little is known about microbial degradation in contaminated regions. we report here that a new bacterial strain isolated from soil, designated dg-02, was shown to degrade 95.6% of 50 mg·l(-1) 3-pba within 72 h in mineral salt medium (msm). strain dg-02 was identified as bacillus sp. based on the morphology, physio-biochemical tests and 16s ... | 2012 | 23226289 |
| pollution impacts on bacterioplankton diversity in a tropical urban coastal lagoon system. | despite a great number of published studies addressing estuarine, freshwater and marine bacterial diversity, few have examined urban coastal lagoons in tropical habitats. there is an increasing interest in monitoring opportunistic pathogens as well as indigenous microbial community members in these water bodies by current molecular and microbiological approaches. in this work, bacterial isolates were obtained through selective plate dilution methods to evaluate antibiotic resistances. in additio ... | 2012 | 23226484 |
| stable isotope probing in the metagenomics era: a bridge towards improved bioremediation. | microbial biodegradation and biotransformation reactions are essential to most bioremediation processes, yet the specific organisms, genes, and mechanisms involved are often not well understood. stable isotope probing (sip) enables researchers to directly link microbial metabolic capability to phylogenetic and metagenomic information within a community context by tracking isotopically labeled substances into phylogenetically and functionally informative biomarkers. sip is thus applicable as a to ... | 2012 | 23022353 |
| stable isotope probing in the metagenomics era: a bridge towards improved bioremediation. | microbial biodegradation and biotransformation reactions are essential to most bioremediation processes, yet the specific organisms, genes, and mechanisms involved are often not well understood. stable isotope probing (sip) enables researchers to directly link microbial metabolic capability to phylogenetic and metagenomic information within a community context by tracking isotopically labeled substances into phylogenetically and functionally informative biomarkers. sip is thus applicable as a to ... | 2012 | 23022353 |
| microbial communities and their performances in anaerobic hybrid sludge bed-fixed film reactor for treatment of palm oil mill effluent under various organic pollutant concentrations. | the anaerobic hybrid reactor consisting of sludge and packed zones was operated with organic pollutant loading rates from 6.2 to 8.2 g cod/l day, composed mainly of suspended solids (ss) and oil and grease (o&g) concentrations between 5.2 to 10.2 and 0.9 to 1.9 g/l, respectively. the overall process performance in terms of chemical oxygen demands (cod), ss, and o&g removals was 73, 63, and 56%, respectively. when the organic pollutant concentrations were increased, the resultant methane potentia ... | 2012 | 22927723 |
| genome sequence of the polychlorinated-biphenyl degrader pseudomonas pseudoalcaligenes kf707. | pseudomonas pseudoalcaligenes kf707 is a soil polychlorinated biphenyl (pcb) degrader, able to grow both planktonically and as a biofilm in the presence of various toxic metals and metalloids. here we report the genome sequence (5,957,359 bp) of p. pseudoalcaligenes kf707, which provides insights into metabolic degradation pathways, flagellar motility, and chemotaxis. | 2012 | 22843571 |
| characterization of biphenyl dioxygenase sequences and activities encoded by the metagenomes of highly polychlorobiphenyl-contaminated soils. | total extracted dna from two heavily polychlorobiphenyl-contaminated soils was analyzed with respect to biphenyl dioxygenase sequences and activities. this was done by pcr amplification and cloning of a dna segment encoding the active site of the enzyme. the translated sequences obtained fell into three similarity clusters (i to iii). sequence identities were high within but moderate or low between the clusters. members of clusters i and ii showed high sequence similarities with well-known biphe ... | 2012 | 22327590 |
| beta-lactams resistance and presence of class 1 integron in pseudomonas spp. isolated from untreated hospital effluents in brazil. | the aim of the present study was to investigate the resistance profile, to detect the presence of beta-lactam resistance genes, phenotypic expression of efflux pump systems and class 1 integrons in pseudomonas spp. strains obtained from untreated hospital effluents. effluent samples were collected from four hospitals in porto alegre, rs, brazil. pseudomonas were isolated on macconkey agar plates and the identification was confirmed by 16s rrna pcr and biochemical tests. susceptibility testing wa ... | 2012 | 22382676 |
| the paax-type repressor meqr2 of arthrobacter sp. strain rue61a, involved in the regulation of quinaldine catabolism, binds to its own promoter and to catabolic promoters and specifically responds to anthraniloyl coenzyme a. | the genes coding for quinaldine catabolism in arthrobacter sp. strain rue61a are clustered on the linear plasmid pal1 in two upper pathway operons (meqabc and meqdef) coding for quinaldine conversion to anthranilate and a lower pathway operon encoding anthranilate degradation via coenzyme a (coa) thioester intermediates. the meqr2 gene, located immediately downstream of the catabolic genes, codes for a paax-type transcriptional repressor. meqr2, purified as recombinant fusion protein, forms a di ... | 2012 | 23275246 |
| the nit1c gene cluster of pseudomonas pseudoalcaligenes cect5344 involved in assimilation of nitriles is essential for growth on cyanide. | a proteomic approach was used to identify several proteins induced by cyanide in the alkaliphilic bacterium pseudomonas pseudoalcaligenes cect5344, two of them, nitb and nitg, encoded by genes that belong to the nit1c gene cluster. the predicted products of the nit1c gene cluster are a fis-like σ(54) -dependent transcriptional activator (nita), a nitrilase (nitc), an s-adenosylmethionine superfamily member (nitd), an n-acyltransferase superfamily member (nite), a trifunctional polypeptide of the ... | 2012 | 23760796 |
| factors influencing the microbial composition of metalworking fluids and potential implications for machine operator's lung. | hypersensitivity pneumonitis, also known as "machine operator's lung" (mol), has been related to microorganisms growing in metalworking fluids (mwfs), especially mycobacterium immunogenum. we aimed to (i) describe the microbiological contamination of mwfs and (ii) look for chemical, physical, and environmental parameters associated with variations in microbiological profiles. we microbiologically analyzed 180 mwf samples from nonautomotive plants (e.g., screw-machining or metal-cutting plants) i ... | 2012 | 22057869 |
| blue-carba, an easy biochemical test for detection of diverse carbapenemase producers directly from bacterial cultures. | 2013 | 24108615 | |
| protective role of the neuropeptide urocortin ii against experimental sepsis and leishmaniasis by direct killing of pathogens. | we currently face an alarming resurgence in infectious diseases characterized by antimicrobial resistance and therapeutic failure. this has generated the urgent need of developing new therapeutic approaches that include agents with nontraditional modes of action. a recent interest focused on approaches based on our natural immune defenses, especially on peptides that combine innate antimicrobial activity against diverse pathogens and immunoregulatory functions. in this study, to our knowledge, w ... | 2013 | 24249730 |
| silaffins of diatoms: from applied biotechnology to biomedicine. | silaffins are involved in the formation of the cell walls of diatoms. it is known that silaffins can precipitate silica in vitro, forming nano- and micro-particles in the shape of spheres and plates containing many pores. it is important to note that the deposition of silica and the particle morphology in the presence of silaffins affects chemical and physical agents (e.g., peptides, polyamines, phosphate, nitrogen, and the mechanical changes of the reaction mixture). it is believed that silaffi ... | 2013 | 24065158 |
| clonal dissemination, emergence of mutator lineages and antibiotic resistance evolution in pseudomonas aeruginosa cystic fibrosis chronic lung infection. | chronic respiratory infection by pseudomonas aeruginosa is a major cause of mortality in cystic fibrosis (cf). we investigated the interplay between three key microbiological aspects of these infections: the occurrence of transmissible and persistent strains, the emergence of variants with enhanced mutation rates (mutators) and the evolution of antibiotic resistance. for this purpose, 10 sequential isolates, covering up to an 8-year period, from each of 10 cf patients were studied. as anticipate ... | 2013 | 23951065 |
| pseudomonas pseudoalcaligenes peritoneal dialysis-associated peritonitis. | 2013 | 23478381 | |
| molecular characterization of blandm-1 in a sequence type 235 pseudomonas aeruginosa isolate from france. | an ndm-1 carbapenemase-producing pseudomonas aeruginosa isolate was recovered from a patient hospitalized in france after a previous hospitalization in serbia. genetic studies revealed that the blandm-1 gene was surrounded by insertion sequence isaba125 and a truncated bleomycin resistance gene. this blandm-1 region was a part of the variable region of a new complex class 1 integron bearing is common region 1 (iscr1). the presence of ispa7 upstream of this integron suggests insertion in a chromo ... | 2013 | 23612200 |
| [effect of lipolytic and catalase activity on physico-mechanical properties of coating polyken 980-25]. | lipolytic and catalase activity of pseudomonas pseudoalcaligenes 109, rhodococcus erythropolis 102, bacillus subtilis 138 and their association with different growth models: biofilm and plankton ones. it is shown that under biofilm conditions the fermentative activity of bacteria under study was 1.5-1.7 times higher than under plankton conditions. monocultures of bacteria displayed much lower activity than associative ones. changes of physico-chemical properties of the specimens of protective co ... | 2013 | 23516839 |
| diversity of chlorobiphenyl-metabolizing bacteria and their biphenyl dioxygenases in contaminated sediment. | bacteria and bacterial communities in sites contaminated with polychlorinated biphenyls have been extensively studied in the past decades. however, there are still major gaps in the knowledge of environmental processes, especially in the behavior of previously described bacteria in vitro, their real degradation abilities and the enzymes that are involved in the degradation processes. in this work we analyzed actively degrading bacterial populations by stable isotope probing with (13)c biphenyl a ... | 2013 | 24007621 |
| metabolic and molecular methods to evaluate the organoclay effects on a bacterial community. | the aim of this work was to evaluate the influence exerted by two different commercial organoclays (dellite 43b and dellite 67g) on a model microbial consortium using microbial metabolic characterization with biolog system and denaturing gradient gel electrophoresis (dgge) molecular approach. the information obtained from the molecular analyses, in their complex, account for the differences in species composition induced on the reference consortium by the contact with the organoclays under study ... | 2013 | 23644274 |
| effect of aluminium and copper on biofilm development of pseudomonas pseudoalcaligenes kf707 and p. fluorescens as a function of different media compositions. | bioremediation efforts worldwide are faced with the problem of metals interfering with the degradation of organic pollutants. there has been little systematic investigation into how the important environmental factors of media composition, buffering agent, and carbon source affect the exertion of metal toxicity on bacteria. this study aimed to systematically separate and investigate the influence of these factors by examining planktonic and biofilm establishment and growth. two pseudomonads were ... | 2013 | 23604327 |
| draft whole genome sequence of the cyanide-degrading bacterium pseudomonas pseudoalcaligenes cect5344. | pseudomonas pseudoalcaligenes cect5344 is a gram-negative bacterium able to tolerate cyanide and to use it as the sole nitrogen source. we report here the first draft of the whole genome sequence of a p. pseudoalcaligenes strain that assimilates cyanide. three aspects are specially emphasized in this manuscript. first, some generalities of the genome are shown and discussed in the context of other pseudomonadaceae genomes, including genome size, g + c content, core genome and singletons among ot ... | 2013 | 22998548 |
| genome sequence of a strain of the human pathogenic bacterium pseudomonas alcaligenes that caused bloodstream infection. | pseudomonas alcaligenes, a gram-negative aerobic bacterium, is a rare opportunistic human pathogen. here, we report the whole-genome sequence of p. alcaligenes strain mry13-0052, which was isolated from a bloodstream infection in a medical institution in japan and is resistant to antimicrobial agents, including broad-spectrum cephalosporins and monobactams. | 2013 | 24179116 |
| structural and enzymatic characterization of the phosphotriesterase ophc2 from pseudomonas pseudoalcaligenes. | organophosphates (ops) are neurotoxic compounds for which current methods of elimination are unsatisfactory; thus bio-remediation is considered as a promising alternative. here we provide the structural and enzymatic characterization of the recently identified enzyme isolated from pseudomonas pseudoalcaligenes dubbed ophc2. ophc2 belongs to the metallo-β-lactamase superfamily and exhibits an unusual thermal resistance and some op degrading abilities. | 2013 | 24223749 |
| biodegradation potential of cyano-based ionic liquid anions in a culture of cupriavidus spp. and their in vitro enzymatic hydrolysis by nitrile hydratase. | biodegradation tests with bacteria from activated sludge revealed the probable persistence of cyano-based ionic liquid anions when these leave waste water treatment plants. a possible biological treatment using bacteria capable of biodegrading similar compounds, namely cyanide and cyano-complexes, was therefore examined. with these bacteria from the genera cupriavidus, the ionic liquid anions b(cn)₄(-), c(cn)₃(-), n(cn)₂(-) combined with alkaline cations were tested in different growth media usi ... | 2013 | 24338070 |
| biodegradation potential of cyano-based ionic liquid anions in a culture of cupriavidus spp. and their in vitro enzymatic hydrolysis by nitrile hydratase. | biodegradation tests with bacteria from activated sludge revealed the probable persistence of cyano-based ionic liquid anions when these leave waste water treatment plants. a possible biological treatment using bacteria capable of biodegrading similar compounds, namely cyanide and cyano-complexes, was therefore examined. with these bacteria from the genera cupriavidus, the ionic liquid anions b(cn)₄(-), c(cn)₃(-), n(cn)₂(-) combined with alkaline cations were tested in different growth media usi ... | 2013 | 24338070 |
| influence of root exudates on the bacterial degradation of chlorobenzoic acids. | degradation of chlorobenzoic acids (e.g., products of microbial degradation of pcb) by strains of microorganisms isolated from pcb contaminated soils was assessed. from seven bulk-soil isolates two strains unique in ability to degrade a wider range of chlorobenzoic acids than others were selected, individually and even in a complex mixture of 11 different chlorobenzoic acids. such a feature is lacking in most tested degraders. to investigate the influence of vegetation on chlorobenzoic acids deg ... | 2013 | 24222753 |
| mechanisms of antimicrobial resistance in finfish aquaculture environments. | consumer demand for affordable fish drives the ever-growing global aquaculture industry. the intensification and expansion of culture conditions in the production of several finfish species has been coupled with an increase in bacterial fish disease and the need for treatment with antimicrobials. understanding the molecular mechanisms of antimicrobial resistance prevalent in aquaculture environments is important to design effective disease treatment strategies, to prioritize the use and registra ... | 2013 | 23986749 |
| host-microbe interactions in caenorhabditis elegans. | a good understanding of how microbes interact with hosts has a direct bearing on our capability of fighting infectious microbial pathogens and making good use of beneficial ones. among the model organisms used to study reciprocal actions among microbes and hosts, c. elegans may be the most advantageous in the context of its unique attributes such as the short life cycle, easiness of laboratory maintenance, and the availability of different genetic mutants. this review summarizes the recent advan ... | 2013 | 23984180 |
| novel pcr-based methods enhance characterization of vaginal microbiota in a bacterial vaginosis patient before and after treatment. | deep characterization, even by next-generation sequencing, of the vaginal microbiota in healthy women or posttreatment bacterial vaginosis patients is limited by the dominance of lactobacilli. to improve detection, we offer two approaches: quantitative pcr (qpcr) using phylogenetic branch-inclusive primers and sequencing of broad-spectrum amplicons generated with oligomers that block amplification of lactobacilli. | 2013 | 23624483 |
| association with soil bacteria enhances p38-dependent infection resistance in caenorhabditis elegans. | the importance of our inner microbial communities for proper immune responses against invading pathogens is now well accepted, but the mechanisms underlying this protection are largely unknown. in this study, we used caenorhabditis elegans to investigate such mechanisms. since very little is known about the microbes interacting with c. elegans in its natural environment, we began by taking the first steps to characterize the c. elegans microbiota. we established a natural-like environment in whi ... | 2013 | 23230286 |
| plant growth-promoting rhizobacteria and root system functioning. | the rhizosphere supports the development and activity of a huge and diversified microbial community, including microorganisms capable to promote plant growth. among the latter, plant growth-promoting rhizobacteria (pgpr) colonize roots of monocots and dicots, and enhance plant growth by direct and indirect mechanisms. modification of root system architecture by pgpr implicates the production of phytohormones and other signals that lead, mostly, to enhanced lateral root branching and development ... | 2013 | 24062756 |
| cyanuric acid hydrolase: evolutionary innovation by structural concatenation. | the cyanuric acid hydrolase, atzd, is the founding member of a newly identified family of ring-opening amidases. we report the first x-ray structure for this family, which is a novel fold (termed the 'toblerone' fold) that likely evolved via the concatenation of monomers of the trimeric yjgf superfamily and the acquisition of a metal binding site. structures of atzd with bound substrate (cyanuric acid) and inhibitors (phosphate, barbituric acid and melamine), along with mutagenesis studies, allo ... | 2013 | 23651355 |
| the three-species consortium of genetically improved strains cupriavidus necator rw112, burkholderia xenovorans rw118, and pseudomonas pseudoalcaligenes rw120 grows with technical polychlorobiphenyl, aroclor 1242. | burkholderia xenovorans lb400, cupriavidus necator h850, and pseudomonas pseudoalcaligenes kf707 are bacterial strains able to mineralize biphenyl and to co-oxidize many of its halogenated derivatives (pcbs). only strain lb400 also mineralizes a few mono- and dichlorobiphenyls, due to the presence of a functioning chlorocatechol pathway. here, we used a tn5-based minitransposon shuttle system to chromosomically introduce genes tcbrcdef, encoding the chlorocatechol pathway into kf707, and genes c ... | 2013 | 23658554 |
| the novel bacterial n-demethylase pdmab is responsible for the initial step of n,n-dimethyl-substituted phenylurea herbicide degradation. | the environmental fate of phenylurea herbicides has received considerable attention in recent decades. the microbial metabolism of n,n-dimethyl-substituted phenylurea herbicides can generally be initiated by mono-n-demethylation. in this study, the molecular basis for this process was revealed. the pdmab genes in sphingobium sp. strain ybl2 were shown to be responsible for the initial mono-n-demethylation of commonly used n,n-dimethyl-substituted phenylurea herbicides. pdmab is the oxygenase com ... | 2013 | 24123738 |
| genomic and functional analyses of the 2-aminophenol catabolic pathway and partial conversion of its substrate into picolinic acid in burkholderia xenovorans lb400. | 2-aminophenol (2-ap) is a toxic nitrogen-containing aromatic pollutant. burkholderia xenovorans lb400 possess an amn gene cluster that encodes the 2-ap catabolic pathway. in this report, the functionality of the 2-aminophenol pathway of b. xenovorans strain lb400 was analyzed. the amnrjbacdfehg cluster located at chromosome 1 encodes the enzymes for the degradation of 2-aminophenol. the absence of haba and habb genes in lb400 genome correlates with its no growth on nitrobenzene. rt-pcr analyses ... | 2013 | 24124510 |
| characterizing the promiscuity of ligab, a lignin catabolite degrading extradiol dioxygenase from sphingomonas paucimobilis syk-6. | ligab from sphingomonas paucimobilis syk-6 is the only structurally characterized dioxygenase of the largely uncharacterized superfamily of type ii extradiol dioxygenases (edo). this enzyme catalyzes the oxidative ring-opening of protocatechuate (3,4-dihydroxybenzoic acid or pca) in a pathway allowing the degradation of lignin derived aromatic compounds (ldacs). ligab has also been shown to utilize two other ldacs from the same metabolic pathway as substrates, gallate, and 3-o-methyl gallate; ho ... | 2013 | 23977959 |
| three-component o-demethylase system essential for catabolism of a lignin-derived biphenyl compound in sphingobium sp. strain syk-6. | sphingobium sp. strain syk-6 is able to assimilate lignin-derived biaryls, including a biphenyl compound, 5,5'-dehydrodivanillate (ddva). previously, ligxa (slg_07770), which is similar to the gene encoding oxygenase components of rieske-type nonheme iron aromatic-ring-hydroxylating oxygenases, was identified to be essential for the conversion of ddva; however, the genes encoding electron transfer components remained unknown. disruption of putative electron transfer component genes scattered thr ... | 2014 | 25217011 |
| evaluation of the bruker biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry system for identification of blood isolates of acinetobacter species. | matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) (bruker biotyper) was able to accurately identify 98.6% (142/144) of acinetobacter baumannii isolates, 72.4% (63/87) of a. nosocomialis isolates, and 97.6% (41/42) of a. pittii isolates. all acinetobacter junii, a. ursingii, a. johnsonii, and a. radioresistens isolates (n = 28) could also be identified correctly by bruker biotyper. | 2014 | 24899038 |
| biodegradation of cyanide by a new isolated strain under alkaline conditions and optimization by response surface methodology (rsm). | biodegradation of free cyanide from industrial wastewaters has been proven as a viable and robust method for treatment of wastewaters containing cyanide. | 2014 | 24921051 |
| proteomic strategy for the analysis of the polychlorobiphenyl-degrading cyanobacterium anabaena pd-1 exposed to aroclor 1254. | the cyanobacterium anabaena pd-1, which was originally isolated from polychlorobiphenyl (pcb)-contaminated paddy soils, has capabilities for dechlorinatin and for degrading the commercial pcb mixture aroclor 1254. in this study, 25 upregulated proteins were identified using 2d electrophoresis (2-de) coupled with matrix-assisted laser desorption/ionization time of flight mass spectrometry (maldi-tof ms). these proteins were involved in (i) pcb degradation (i.e., 3-chlorobenzoate-3,4-dioxygenase); ... | 2014 | 24618583 |
| microbial diversity similarities in periodontal pockets and atheromatous plaques of cardiovascular disease patients. | the immune and infectious alterations occurring in periodontitis have been shown to alter the development and severity of cardiovascular disease. one of these relationships is the translocation of oral bacteria to atheroma plaques, thereby promoting plaque development. thus, the aim of this study was to assess, by 16s cloning and sequencing, the microbial diversity of the subgingival environment and atheroma plaques of patients concomitantly suffering from periodontitis and obstructive coronary ... | 2014 | 25329160 |
| arhodomonas sp. strain seminole and its genetic potential to degrade aromatic compounds under high-salinity conditions. | arhodomonas sp. strain seminole was isolated from a crude oil-impacted brine soil and shown to degrade benzene, toluene, phenol, 4-hydroxybenzoic acid (4-hba), protocatechuic acid (pca), and phenylacetic acid (paa) as the sole sources of carbon at high salinity. seminole is a member of the genus arhodomonas in the class gammaproteobacteria, sharing 96% 16s rrna gene sequence similarity with arhodomonas aquaeolei ha-1. analysis of the genome predicted a number of catabolic genes for the metabolis ... | 2014 | 25149520 |