Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| growth of paracoccus denitrificans on [2,3-13c]succinate and [1,4-13c]succinate. ii. isoleucine biosynthesis. | paracoccus denitrificans was grown on either [2,3-13c]succinate or [1,4-13c]succinate, and extracts were analysed by using gas chromatography-mass spectrometry. the distribution of label in isoleucine indicated that the 2-ketobutyrate required for isoleucine biosynthesis was mainly produced from pyruvate by 2-keto-acid chain elongation (i.e. the 'pyruvate elongation pathway'). approximately 10% of isoleucine was produced by a second pathway involving propionyl coa. threonine and glutamate were n ... | 1987 | 2888122 |
| subunit ii of cytochrome c oxidase from paracoccus denitrificans. dna sequence, gene expression and the protein. | cytochrome c oxidase from the bacterium paracoccus denitrificans, while being related to the mitochondrial enzyme in many ways, consists of only two to three different subunits. for the identification of its genes, a paracoccus dna library was constructed and screened with specific antibodies for expression of cloned inserts in e. coli. a positive clone expressing immunoreactive products in the molecular mass region of authentic subunit ii revealed a high homology of its dna-deduced amino acid s ... | 1987 | 2820725 |
| the genes of the paracoccus denitrificans bc1 complex. nucleotide sequence and homologies between bacterial and mitochondrial subunits. | the genes for the three subunits of the cytochrome bc1 complex from the bacterium paracoccus denitrificans were identified by screening a gene library constructed in pbr 322 for expression using a cytochrome c1-specific antibody. these three genes coding for the fes subunit, cytochrome b, and cytochrome c1 were located on contiguous sites on the genome in a presumed operon arrangement. the dna-deduced amino acid sequence shows that all three subunits are homologous to corresponding polypeptides ... | 1987 | 2820981 |
| control of respiration rate in non-growing cells of paracoccus denitrificans. | by means of fluorimetric measurement and by direct determination of intracellular nad+ and nadh contents, it was proved that the respiration rate of paracoccus denitrificans cells utilizing glucose is limited by processes preceding nadh oxidation in the respiratory chain, so that the membrane nadh dehydrogenase is not saturated by its substrate. in the separated membrane fraction on saturation with exogenous nadh the main limiting factor is represented by nadh: ubiquinone oxidoreductase. | 1987 | 2825653 |
| immunological and spectral characterization of partly purified cytochrome oxidase from the cyanobacterium synechocystis 6714. | membranes were isolated by french pressure cell extrusion of lysozyme-preincubated cells of the cyanobacterium synechocystis 6714 after growth in the presence of 0.4 m nacl for 4 days. these cells showed up to 6-fold respiratory activity (oxygen uptake) when compared to control cells. separation of plasma and thylakoid membranes revealed that the major part of cytochrome c oxidase was associated with the latter. immunoblotting of sodium dodecylsulfate polyacrylamide gel electrophorized membranes ... | 1987 | 2825695 |
| amino acid sequences of cytochrome c-554(548) and cytochrome c' from a halophilic denitrifying bacterium of the genus paracoccus. | the amino acid sequences of the cytochromes c-554(548) and c' from the moderately halophilic bacterium paracoccus sp., i.a.m. 203 (= a.t.c.c. 12084, n.c.i.b. 8669) have been determined. cytochrome c-554(548) consists of a single polypeptide chain of 83 residues, and dimerizes strongly. the most similar protein of known sequence is the n-terminal half of the dihaem cytochrome c4, and other related proteins include the cytochrome c-554(547) of thiobacillus neapolitanus and the cytochrome c-553 of ... | 1987 | 2829828 |
| detergent inhibition of nitric-oxide reductase activity. | gas chromatography revealed that exposure of extracts of the denitrifiers 'achromobacter cycloclastes', paracoccus denitrificans, pseudomonas aeruginosa and pseudomonas perfectomarina to triton x-100 inhibited reduction of no to n2o, and thus concomitantly inhibited reduction of no2- to n2o. after exposure of extracts to triton x-100, the ratio of h+ consumed to no2- added decreased from approx. 2.0 (for untreated extracts) to approx. 1.5, which indicated that no2- was reduced to no by the treat ... | 1987 | 3028488 |
| a simple, one-step purification of cytochrome b from the bc1 complexes of bacteria. | 1987 | 3030803 | |
| cytochrome c oxidase is a three-copper, two-heme-a protein. | metal contents of preparations of procaryotic (paracoccus denitrificans) and eucaryotic (beef heart) cytochrome c oxidases have been determined by inductively coupled plasma atomic emission spectroscopy and shown to be stoichiometrically related to the protein contents. the results show that oxidases which possess subunits i and ii have three copper atoms besides hemes a and a3 (paracoccus denitrificans, cu: 2.97 +/- 0.08 and fe: 2.09 +/- 0.10; bovine heart, cu: 2.83 +/- 0.07 and fe: 1.94 +/- 0. ... | 1987 | 3032614 |
| homology between bacterial dna and bovine mitochondrial dna encoding cytochrome c oxidase subunit iii. | a segment of mitochondrial dna encoding the bovine cytochrome c oxidase subunit iii gene was isolated and inserted into an escherichia coli plasmid vector. a 556 base pair fragment of the insert dna representing about 70% of the 3'-end of the subunit iii gene was used to search for homology with bacterial dna from strains that contain heme aa3-type cytochrome c oxidases. bacillus subtilis, thermus thermophilus, and ps3 dnas all showed strong hybridization to the probe, whereas paracoccus denitri ... | 1987 | 3032681 |
| purification and some characteristics of nitrous oxide reductase from paracoccus denitrificans. | nitrous oxide reductase from the denitrifying bacterium paracoccus denitrificans has been purified very nearly to homogeneity by an anaerobic procedure that results in a product with high specific activity. the enzyme is a dimer of about mr 144,000 composed of two subunits of apparently equal mr and contains 4 mol of cu per mol of subunit. the isoelectric point is 4.3; specific activity at 25 degrees c, ph 7.1, is 122 mumol x min-1 x mg of protein-1; and km is about 7 microm n2o under the same c ... | 1987 | 3032972 |
| isolation and characterization of ubiquinol oxidase complexes from paracoccus denitrificans cells cultured under various limiting growth conditions in the chemostat. | to obtain more information about the composition of the respiratory chain under different growth conditions and about the regulation of electron-transfer to several oxidases and reductases, ubiquinol oxidase complexes were partially purified from membranes of paracoccus denitrificans cells grown in carbon-source-limited aerobic, nitrate-limited anaerobic and oxygen-limited chemostat cultures. the isolated enzymes consisted of cytochromes bc1, c552 and aa3. in comparison with the aerobic ubiquino ... | 1987 | 3036512 |
| subfractionation and characterization of soluble c-type cytochromes from paracoccus denitrificans cultured under various limiting conditions in the chemostat. | soluble c-type cytochromes were partially purified from paracoccus denitrificans cells grown in succinate- and methanol-limited aerobic, nitrate-limited anaerobic and oxygen-limited chemostat cultures. five c types could be distinguished with the following apparent molecular masses, absorption maxima and midpoint potentials. (a) 9.2 kda, 549 nm and +190 mv; (b) 14 kda, 549 nm and +227 mv; (c) 22 kda, 552 nm and +190 mv; (d) 30 kda, 552.7 nm and +160 mv; (e) 45 kda, a dihaem: 555 nm, +128 mv and ... | 1987 | 3036513 |
| separate binding sites for antimycin and mucidin in the respiratory chain of the bacterium paracoccus denitrificans and their occurrence in other denitrificans bacteria. | by means of the method of fluorimetric titration it has been shown that mucidin does not affect the attachment of antimycin to membranes from anaerobically grown paracoccus denitrificans. the fluorimetric titration with antimycin can be used in the determination of the amount of the cytochrome bc1 complex in the membrane. in cells inhibited with antimycin, the oxidation of cytochromes c was accompanied by the reduction of cytochrome b; in the presence of mucidin this effect did not take place. t ... | 1988 | 2844159 |
| investigation by electron paramagnetic resonance spectroscopy of the molybdenum centre of respiratory nitrate reductase from paracoccus denitrificans. | the molybdenum centre of respiratory nitrate reductase from paracoccus denitrificans has been investigated by e.p.r. spectroscopy of mo(v). in common with the centres of the analogous enzymes from escherichia coli and pseudomonas aeruginosa, it undergoes a ph- and anion-dependent transition between two different e.p.r. signal-giving species. comparison of the relevant e.p.r. parameters extracted with the help of computer simulations reveals ligation of the metal in the active centres of the thre ... | 1988 | 2844161 |
| subunit i is the catalytic center of paracoccus denitrificans cytochrome c oxidase. | 1988 | 2854385 | |
| subunit ii of the paracoccus denitrificans cytochrome c oxidase. expression studies of its cloned gene in e. coli. | 1988 | 2854402 | |
| cytochrome o from escherichia coli is structurally related to cytochrome aa3. | 1988 | 2854403 | |
| some spectroscopic views of the cua site in cytochrome c oxidase preparations. | 1988 | 2854410 | |
| resonance raman spectroscopy of amicyanin, a blue copper protein from paracoccus denitrificans. | the copper binding site of amicyanin from paracoccus denitrificans has been examined by resonance raman spectroscopy. the pattern of vibrational modes is clearly similar to those of the blue copper proteins azurin and plastocyanin. intense resonance-enhanced peaks are observed at 377, 392, and 430 cm-1 as well as weaker overtones and combination bands in the high frequency region. most of the peaks below 500 cm-1 shift 0.5-1.5 cm-1 to lower energy when the protein is exposed to d2o. based on the ... | 1988 | 2830281 |
| electrophoretically monodisperse cytochrome c oxidases. | a discontinuous gradient polyacrylamide gel electrophoresis under nondenaturing conditions has been used to demonstrate monodispersity of procaryotic and eucaryotic cytochrome c oxidase preparations. alkaline treated bovine enzyme which contains nine subunits as analysed by subsequent discontinuous sds-polyacrylamide gel electrophoresis is a monodisperse dimer in 0.1% triton x-100 and a monomer in 0.1% dodecyl maltoside. the mr-values corrected for bound detergent are 286,000 in triton x-100 and ... | 1988 | 2831897 |
| growth of paracoccus denitrificans on [2,3-13c]succinate and [1,4-13c]succinate. iii. biosynthetic pathways. | the biosynthesis in vivo of a number of amino acids, sugars, and purines in paracoccus denitrificans grown on either [2,3-13c]succinate or [1,4-13c]succinate was investigated by using gas chromatography-mass spectrometry. the distribution of label in the tca-cycle-related amino acids indicated that carbon intermediates of energy metabolism were utilized as precursors for the biosynthesis of these amino acids in vivo. the biosynthesis of glycine, serine, phenylalanine and glycerol from labelled s ... | 1988 | 2895930 |
| intraliposomal nucleotides change the kinetics of reconstituted cytochrome c oxidase from bovine heart but not from paracoccus denitrificans. | isolated cytochrome c oxidases of p. denitrificans and bovine heart were reconstituted in liposomes and the kinetics of cytochrome c oxidation were measured in the presence and absence of nucleotides either inside or outside of proteoliposomes, and after photolabelling with 8-azido-atp. intraliposomal atp increases and adp decreases the kinetics of ferrocytochrome c oxidation of the bovine but not of the paracoccus enzyme. extra-liposomal atp and adp increase the km for cytochrome c of both enzy ... | 1988 | 2838021 |
| evolution of cytochrome c oxidase. | the current view on the regulatory function of nuclear-encoded subunits of cytochrome c oxidase from higher evolved organisms is presented. the activity of monomeric laurylmaltoside-dissolved, but not of reconstituted cytochrome c oxidase, is strongly affected by anions accompanied by a conformational change of the enzyme, as shown by changed visible spectra. addition of uncoupler to proteoliposomes induces the same anion sensitivity as obtained with the soluble enzyme, suggesting dissociation o ... | 1988 | 2841683 |
| subunit iii of cytochrome c oxidase is expressed in paracoccus denitrificans. | polyclonal antibodies have been obtained against a synthetic dodecapeptide identical to the aminoacid sequence 120-131 dspikdgvwppe (inferred from its dna sequence) of paracoccus denitrificans cytochrome c oxidase subunit iii. the antibodies had a titer higher than 1:10000 when tested against the antigen. these antibodies have been used to produce immunological evidence that, despite the fact that subunit iii is not isolated with cytochrome c oxidase, it exists in paracoccus denitrificans lysate ... | 1988 | 2841930 |
| proteolysis of paracoccus denitrificans cytochrome oxidase by trypsin and chymotrypsin. | paracoccus oxidase containing only two subunits was subjected to proteolysis by trypsin and chymotrypsin. both subunits of the purified enzyme were cleaved at only a few sites and enzymatic activity was not inhibited. the cleavage sites were identified by protein sequencing. subunit i was cleaved near the amino-terminus and subunit ii in the loop connecting the two predicted trans-membrane helices. in native membrane fragments, but not in intact spheroplasts, this loop was accessible to both pro ... | 1988 | 2842192 |
| cytochrome c oxidase from paracoccus denitrificans: both hemes are located in subunit i. | the two-subunit cytochrome c oxidase from paracoccus denitrificans has been sequentially digested with chymotrypsin and staphylococcus aureus v8 protease. the smaller subunit of the enzyme (apparent mr 32,000) was split into numerous peptides that were removed by anion-exchange hplc. the larger subunit was only digested to a limited extent (from an apparent mr 45,000 to mr 43,000), and the spectral properties were preserved relative to the native enzyme (a reduced minus oxidized difference spect ... | 1988 | 2842784 |
| stable isotope evidence for the localisation of some metabolic pathways in the bacterium paracoccus denitrificans. | paracoccus denitrificans was grown on [6-13c]-glucose as the sole carbon source for growth and the extracts were fractionated and analysed by gas chromatography-mass spectrometry. the 13c-enrichments of some metabolites indicated that the "hydrolysate pools" of these metabolites were not in isotopic equilibrium with the water soluble "free pools". it was concluded that localisation of some metabolic pathways had occurred in paracoccus during growth on [6-13c]-glucose. | 1988 | 3134030 |
| physiological regulation of paracoccus denitrificans methanol dehydrogenase synthesis and activity. | an enzyme-linked immunosorbent assay and a whole-cell activity assay were developed which allowed detection of methanol dehydrogenase (mdh) of paracoccus denitrificans with increased sensitivity. by these methods, it was shown that mdh was not induced by its natural substrate, methanol. relief from a catabolite repression-like mechanism seemed responsible for low-level mdh synthesis, while product induction was the hypothesized mechanism for synthesis of high amounts of mdh. in the latter proces ... | 1988 | 3042759 |
| different lipid a types in lipopolysaccharides of phototrophic and related non-phototrophic bacteria. | lipid a analyses confirm not only the present taxa of the purple nonsulfur bacteria (formerly rhodospirillaceae), but also phylogenetical relatedness of distinct phototrophic to distinct non-phototrophic bacteria, as was suggested by cataloguing 16s rrna. for example, lipid a with ester-bound 3-oh-10:0 and the rare amide-linked 3-oxo-14:0 is common to the phototrophic rhodobacter capsulatus and rhodobacter sphaeroides and also to paracoccus denitrificans and thiobacillus versutus. 'lipid adag' ( ... | 1988 | 3078741 |
| a bacteriophage t4 expression cassette that functions efficiently in a wide range of gram-negative bacteria. | we have constructed a derivative of the broad-host-range vector rsf1010. this plasmid, p alpha omega, contains an expression cassette derived from bacteriophage t4 gene 32, into which we have inserted the coding sequence for the xyle enzyme (c2,3o) of the tol plasmid pwwo. the composite plasmid, p alpha xyle omega, was transferred by conjugal mobilisation into a variety of gram-negative bacteria (agrobacter, paracoccus, erwinia, pseudomonas, rhizobium and xanthomonas). high levels of c2,3o activ ... | 1988 | 3259198 |
| trapping of nitric oxide produced during denitrification by extracellular hemoglobin. | a spectrophotometric method has been developed that uses extracellular hemoglobin (hb) to trap nitric oxide (no) released during denitrification as nitrosyl hemoglobin (hbno). the rate of complexation of no with hb is about at the diffusion controlled limit for protein molecules and the product, hbno, is essentially stable. hb was added to an anaerobic bacterial suspension and denitrification was initiated with either kno2 or kno3. hbno formation was observed for six species of denitrifying bact ... | 1988 | 3339013 |
| 19f-nuclear magnetic resonance: measurements of [o2] and ph in biological systems. | 19f-nuclear magnetic resonance (nmr) has been used to determine both intracellular ph and oxygen concentrations in cell suspensions. oxygen concentrations in paracoccus denitrificans and insulinoma cells, rinm5f, in the nmr probe can be monitored directly by 1/t1 measurements of perfluorotripropylamine (ftpa)/lecithin emulsion added to the suspensions. with ftpa oxygen monitoring, we investigated the relative aeration capabilities of two types of nmr chambers. both normal and transformed eucaryo ... | 1988 | 3345331 |
| respiratory nitrate reductase from paracoccus denitrificans. evidence for two b-type haems in the gamma subunit and properties of a water-soluble active enzyme containing alpha and beta subunits. | 1. the b-type haem centres of the three (alpha, beta and gamma) subunit nitrate reductase from paracoccus denitrificans have been analysed by redox potentiometry. two components were identified with mid-point potentials +95 mv and +210 mv. 2. washing, in the absence of mg2+ ions, of cytoplasmic membrane vesicles from p. denitrificans promoted selective release of nitrate reductase activity. the released enzyme was purified by chromatography and shown to contain alpha and beta, but not gamma poly ... | 1988 | 3371362 |
| increased outer membrane ornithine-containing lipid and lysozyme penetrability of paracoccus denitrificans grown in a complex medium deficient in divalent cations. | paracoccus denitrificans grown in a complex medium was highly susceptible to lysozyme, in contrast to cells grown in a complex medium supplemented with mg2+ and ca2+ or in a succinate-salts medium. the complex medium was deficient in divalent cations needed for optimum outer membrane stability. the major change in molecular compositions of outer membranes isolated from cells grown under the different conditions was a higher ratio of ornithine-containing lipid to phospholipid in complex-medium-gr ... | 1988 | 3384812 |
| stable isotope evidence for the localisation of phenylalanine biosynthesis in the bacterium paracoccus denitrificans. | paracoccus denitrificans was grown on [6-13c]-glucose as the sole carbon source for growth and the extracts were fractionated and analysed by gas chromatography-mass spectrometry. the 13c-labelling pattern observed in phenylalanine indicated that the biosynthetic sequences of enzymes for phenylalanine production were unequally distributed within the cell and that there are at least 2 separate loci of phenylalanine biosynthesis. the principal locus of phenylalanine production was associated with ... | 1988 | 3390200 |
| demonstration of ferric l-parabactin-binding activity in the outer membrane of paracoccus denitrificans. | under low-iron conditions, paracoccus denitrificans excretes a catecholamine siderophore, l-parabactin, to sequester and utilize iron. in this report, we demonstrate the presence of stereospecific high-affinity ferric l-parabactin-binding activity associated with p. denitrificans membranes grown in low-iron medium. isolated outer membrane components were shown to be three to four times higher in specific activity for ferric l-parabactin. the same amount of binding activity existed whether or not ... | 1988 | 3403511 |
| [formation of a methylotrophic denitrifying biocenosis in a system of sewage treatment for nitrates]. | a methylotrophic denitrifying bioenosis composed of hyphomicrobes and paracocci was isolated from the active ooze in a system of sewage purification from nitrates. the morphological and physiological characteristics of the isolated hyphomicrobium sp. z-115 and paracoccus denitrificans z-100 and z-121 strains differed from those of the type strains, which made it difficult to identify them and to isolate them as a pure culture. this should be taken into account while determining the agents operat ... | 1988 | 3419370 |
| preparation of a one-subunit cytochrome oxidase from paracoccus denitrificans: spectral analysis and enzymatic activity. | cytochrome c oxidase was isolated from paracoccus denitrificans as a two-subunit enzyme. chymotrypsin-catalyzed proteolysis reduced the molecular weight of each subunit by about 8000. the spectral properties of this preparation, as well as its km for cytochrome c(1.7 mum), remained unchanged with respect to the native enzyme. vmax was reduced by about 55% when assayed in triton x-100 or in triton x-100 supplemented with asolectin. following further proteolysis by staphylococcus aureus v8 proteas ... | 1988 | 2462906 |
| complex formation between methylamine dehydrogenase and amicyanin from paracoccus denitrificans. | two proteins isolated from paracoccus denitrificans, the copper-containing electron carrier amicyanin and the pyrroloquinoline quinone-containing enzyme methylamine dehydrogenase, have been shown to form a complex. complex formation between methylamine dehydrogenase and either oxidized or reduced amicyanin resulted in alterations in the absorbance spectrum of the pyrroloquinoline quinone prosthetic group of methylamine dehydrogenase. binding of amicyanin to the enzyme exhibited positive cooperat ... | 1988 | 3170535 |
| preliminary x-ray crystallographic studies of methylamine dehydrogenase and methylamine dehydrogenase--amicyanin complexes from paracoccus denitrificans. | 1988 | 3210240 | |
| kinetics of the interaction of cytochrome c oxidase of paracoccus denitrificans with paracoccus and mitochondrial cytochrome c. | we have studied the reactions of the oxidase of paracoccus dentrificans with its membrane-bound cytochrome c and with soluble cytochrome c550 of paracoccus and of bovine heart. the turnover rate of paracoccus oxidase with membrane-bound cytochrome c is high, approaching 1000/sec. at 25 degrees. when soluble cytochrome c is added to the electron transport chain oxidizing nadh or succinate, no increase in 02 uptake is observed. when the oxidase is reacting with the membrane-bound cytochrome c, the ... | 1988 | 2841681 |
| purification and properties of succinate-ubiquinone oxidoreductase complex from paracoccus denitrificans. | highly active succinate-ubiquinone reductase has been purified from cytoplasmic membranes of aerobically grown paracoccus denitrificans. the purified enzyme has a specific activity of 100 units per mg protein, and a turnover number of 305 s-1. succinate-ubiquinone reductase activity of the purified enzyme is inhibited by 3'-methylcarboxin and thenoyltrifluoroacetone. four subunits, with apparent molecular masses of 64.9, 28.9, 13.4 and 12.5 kda, were observed on sodium dodecyl sulfate polyacryla ... | 1988 | 2843228 |
| the paracoccus denitrificans cytochrome aa3 has a third subunit. | the presence of a third polypeptide subunit in paracoccus cytochrome c oxidase is demonstrated. this protein (apparent molecular mass 23 kda) binds dicyclohexylcarbodiimide in membranes of aerobically grown bacteria and in the purified enzyme. the n-terminal amino-acid sequence of this dicyclohexylcarbodiimide-binding protein is identical to the deduced sequence of the coiii gene product [raitio et al. (1987) embo j. 6, 2825-2833]. we conclude that the aa3-type oxidase in paracoccus is composed ... | 1988 | 2832167 |
| both hemes are located in subunit one of paracoccus denitrificans cytochrome c oxidase. | 1988 | 2841679 | |
| bacterial cytochrome c oxidases: cloning and sequence determination of subunit ii of the paracoccus oxidase. | 1988 | 2841680 | |
| kinetics of the interaction of the cytochrome c oxidase of paracoccus denitrificans with its own and bovine cytochrome c. | we have devised a relatively simple method for the purification of cytochrome aa3 of paracoccus denitrificans with three major subunits similar to those of the larger subunits of the mitochondrial cytochrome oxidase. this preparation has no c-type cytochrome. studies were made of the oxidation of soluble cytochromes c from bovine heart and paracoccus. the cytochrome-c oxidase activity was stimulated by low concentrations of either cytochrome c, providing an explanation for the multiphasic nature ... | 1988 | 2833305 |
| protonmotive q cycle pathway of electron transfer and energy transduction in the three-subunit ubiquinol-cytochrome c oxidoreductase complex of paracoccus denitrificans. | ubiquinol-cytochrome c oxidoreductase (cytochrome bc1) complex from paracoccus denitrificans consists of only three polypeptide subunits (yang, x., and trumpower, b. l. (1986) j. biol. chem. 261, 12282-12289), whereas the analogous complexes of eukaryotic mitochondria consist of nine or more polypeptides (schagger, h., link, t. a., engel, w. d., and von jagow, g. (1986) methods enzymol. 126, 224-237). using the purified three-subunit paracoccus complex we have tested whether this simple cytochro ... | 1988 | 2841340 |
| simultaneously occurring nitrification and denitrification under oxygen gradient by polyelectrolyte complex-coimmobilized nitrosomonas europaea and paracoccus denitrificans cells. | 1988 | 18584619 | |
| [polymer fixed microorganisms for water processing]. | 1989 | 2490194 | |
| [determination of the growth rate of bacteria in monitoring denitrification plants for drinking water processing]. | 1989 | 2490195 | |
| immunological comparison of subunits isolated from various hydrogenases of aerobic hydrogen bacteria. | polyclonal, monospecific antibodies were produced against the two subunits (mr 62,000, and mr 31,000), isolated from the membrane-bound hydrogenase of alcaligenes eutrophus h16. the antibodies (igg fractions) were purified from crude sera by protein a-sepharose cl-4b chromatography. by double immunodiffusion assays and tandem-crossed immunoelectrophoresis the large and the small subunit were demonstrated not to be immunologically related. immunological comparison of these subunits with the four ... | 1989 | 2493816 |
| regulation of methanol dehydrogenase synthesis in paracoccus denitrificans. | the region downstream from the methanol dehydrogenase (mdh) structural gene has been cloned and sequenced. mdh promoter activity have been studied by using a broad-host-range promoter probe vector. | 1989 | 2505667 |
| inhibition of bovine heart mitochondrial and paracoccus denitrificans nadh----ubiquinone reductase by dequalinium chloride and three structurally related quinolinium compounds. | 1. dequalinium chloride (deca) and three related quinolinium compounds inhibit bovine heart mitochondrial and paracoccus denitrificans electron transport activity, with inhibition localized between nadh and ubiquinone in both electron transport chains. 2. structure-activity studies reveal that two quinolinium rings and a long bridging group are necessary for significant inhibition of reduction of artificial electron acceptors and coenzyme q, whereas only one quinolinium ring and a long hydrocarb ... | 1989 | 2515858 |
| the effect of oxygen on denitrification in paracoccus denitrificans and pseudomonas aeruginosa. | denitrification by paracoccus denitrificans and pseudomonas aeruginosa was studied using quadrupole membrane-inlet mass spectrometry to measure simultaneously and continuously dissolved gases. evidence was provided for aerobic denitrification by both species: in the presence of o2, n2o production increased in pa. denitrificans, while that of n2 decreased; with ps. aeruginosa, the concentrations of both n2 and n2o increased on introducing o2 into the gas phase. disappearance of no-3 was monitored ... | 1989 | 2516869 |
| loss of penicillin resistance in paracoccus denitrificans induced by mitomycin c and acridine orange. | in an attempt to eliminate the penicillin resistance gene of p. dentrificans by curing agents, such as acridine orange (ao) and mitomycin c, it was observed that ao treatment caused temporary phenotypic curing where development of sensitivity was a function of concentration of both the curing agent and benzylpenicillin. however, curing with mitomycin produced sensitive clones at a frequency of 6 x 10(-3) and two permanently cured clones were isolated. heavy metal resistance and resistance to oth ... | 1989 | 2517537 |
| electron transport reactions in a cytochrome c-deficient mutant of paracoccus denitrificans. | a mutant of paracoccus denitrificans which is deficient in c-type cytochromes grows aerobically with generation times similar to those obtained with a wild-type strain. the aa3-type oxidase is functional in the mutant as judged by spectrophotometric assays of cytochrome c oxidation using the membrane particles and cytochrome aa3 reduction in whole cells. the cytochrome c oxidase (aa3-type) of the c-less mutant oxidizes soluble cytochrome c at rates equivalent to those obtained with the wild-type ... | 1989 | 2537100 |
| the membrane-bound hydrogenase from paracoccus denitrificans. purification and molecular characterization. | the membrane-bound hydrogenase from paracoccus denitrificans was purified 68-fold with a yield of 14.6%. the final preparation had a specific activity of 161.9 mumol h2 min-1 (mg protein)-1 (methylene blue reduction). purification involved solubilization by triton x-114, phase separation, chromatography on deae-sephacel, ammonium-sulfate precipitation and chromatography on procion-red he-3b-sepharose. gel electrophoresis under denaturing conditions revealed two non-identical subunits with molecu ... | 1989 | 2537196 |
| the amino acid sequence of adenylate kinase from paracoccus denitrificans and its relationship to mitochondrial and microbial adenylate kinases. | the complete amino acid sequence of adenylate kinase (mgatp + amp in equilibrium mgadp + adp) from paracoccus denitrificans has been determined. 1. the s-[14c]carboxymethylated protein was cleaved with clostripain, cyanogen bromide and endoproteinase lys-c; 18, 9 and 6 fragments, respectively, were analyzed. some of these peptides were further degraded by trypsin, staphylococcus aureus v8 protease and carboxypeptidases a and b. the fragments were separated by hplc and sequenced with a gas-phase ... | 1989 | 2537726 |
| cytochrome c-550 mediates electron transfer from inducible periplasmic c-type cytochromes to the cytoplasmic membrane of paracoccus denitrificans. | electron transfer from periplasmic cytochromes c to the membrane-bound respiratory chain has been studied with the isolated cytochromes and membrane preparations from paracoccus denitrificans. when reduced cytochromes were incubated with spheroplasts only the constitutive cytochrome c-550 was rapidly oxidized. the inducible cytochromes c-551i and c-553i were not oxidized at appreciable rates. cytochrome c-550 was able to mediate the transfer of electrons from either cytochrome c-551i or cytochro ... | 1989 | 2538362 |
| purification and some characteristics of nitric oxide reductase-containing vesicles from paracoccus denitrificans. | nitric oxide reductase of paracoccus denitrificans was purified, with the use of 3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate (chapso) detergent, as membrane vesicles of apparent mr = 2-3 x 10(6). fifty percent of the protein was a peptide of mr = 34,000. further fractionation with sodium dodecyl sulfate (sds) resulted in vesicles in which the peptide constituted 90-95% of the protein. this peptide, which is rich in ala, gly, ser, asx, and glx, is considered to be the pept ... | 1989 | 2708379 |
| oxidation of cytochrome c by cytochrome c oxidase: spectroscopic binding studies and steady-state kinetics support a conformational transition mechanism. | the long-known biphasic response of cytochrome c oxidase to the concentration of cytochrome c has been explained, alternatively, by the presence of a catalytic and a regulatory site on the oxidase, by negative cooperativity between adjacent active sites in dimeric oxidase, or by a transition of the enzyme molecule between different conformational states. the three mechanistic hypotheses allow testable predictions about the relationship between substrate binding and steady-state kinetics catalyze ... | 1989 | 2539857 |
| the energy-conserving nitric-oxide-reductase system in paracoccus denitrificans. distinction from the nitrite reductase that catalyses synthesis of nitric oxide and evidence from trapping experiments for nitric oxide as a free intermediate during denitrification. | 1. a clark-type electrode that responds to nitric oxide has been used to show that cytoplasmic membrane vesicles of paracoccus denitrificans have a nitric-oxide reductase activity. nitrous oxide is the reaction product. nadh, succinate or isoascorbate plus 2,3,5,6-tetramethyl-1,4-phenylene diamine can act as reductants. the nadh-dependent activity is resistant to freezing of the vesicles and thus the nadh:nitric-oxide oxidoreductase activity of stored frozen vesicles provides a method for calibr ... | 1989 | 2920732 |
| assimilation of methylamine by paracoccus denitrificans involves formaldehyde transport by a specific carrier. | assimilation of methylamine by paracoccus denitrificans involves the following enzymes: a periplasmic methylamine dehydrogenase, a formaldehyde transport system, cytoplasmic formaldehyde and formate dehydrogenase. formaldehyde transport follows saturation kinetics with a high substrate affinity (km = 7 microm), and is severely inhibited by iodoacetate, cyanide and p-trifluoromethoxy carbonylcyanide phenylhydrazone. expression of the formaldehyde carrier is regulated by the carbon source. | 1989 | 2612879 |
| formate dependent nitrate and nitrite reduction to ammonia by citrobacter freundii and competition with denitrifying bacteria. | citrobacter freundii, paracoccus denitrificans and pseudomonas stutzeri were grown either singly or in mixed culture in anaerobic nitrate or nitrite limited chemostats with formate and/or succinate as electron donors and carbon sources. c. freundii reduced nitrate or nitrite stoichiometrically to ammonia. maximum molar growth yields for nitrate (nitrite) were 15.3 (9.9) g/mol for c. freundii on formate with succinate as carbon source, 15.3 (9.5) g/mol for ps. stutzeri on succinate and 32.3 (20.4 ... | 1989 | 2619287 |
| structure, control and assembly of a bacterial electron transport system as exemplified by paracoccus denitrificans. | 1989 | 2697623 | |
| cytochrome o (bo) is a proton pump in paracoccus denitrificans and escherichia coli. | spheroplasts from aerobically grown wild-type paracoccus denitrificans cells respire with succinate despite specific inhibition of the cytochrome bc1 complex by myxothiazol. coupled to this activity, which involves only b-type cytochromes, there is translocation of 1.5-1.9 h+/e- across the cytoplasmic membrane. similar h+ translocation ratios are observed during oxidation of ubiquinol in spheroplasts from aerobically grown mutants of paracoccus lacking cytochrome c oxidase, or deficient in cytoc ... | 1989 | 2544445 |
| characterization of the cytochrome c oxidase in isolated and purified plasma membranes from the cyanobacterium anacystis nidulans. | functionally intact plasma membranes were isolated from the cyanobacterium (blue-green alga) anacystis nidulans through french pressure cell extrusion of lysozyme/edta-treated cells, separated from thylakoid membranes by discontinuous sucrose density gradient centrifugation, and purified by repeated recentrifugation. origin and identity of the chlorophyll-free plasma membrane fraction were confirmed by labeling of intact cells with impermeant protein markers, [35s]diazobenzenesulfonate and fluor ... | 1989 | 2545245 |
| omegon-km: a transposable element designed for in vivo insertional mutagenesis and cloning of genes in gram-negative bacteria. | to combine the features of the omega interposons with the advantages of in vivo transposition mutagenesis, we have constructed an artificial transposon, called omegon-km. the omegon-km transposon is carried on the plasmid pjff350 which can be conjugally mobilized into a broad range of gram-negative bacteria. omegon-km is flanked, in inverted orientation, by synthetic 28-bp repeats derived from the ends of is1. in addition, each end of omegon-km has the very efficient transcription and translatio ... | 1989 | 2546859 |
| a bacterial c-type cytochrome can be translocated to the periplasm as an apo form; the biosynthesis of cytochrome cd1 (nitrite reductase) from paracoccus denitrificans. | an apo form of cytochrome cd1 (nitrite reductase) of paracoccus denitrificans has been detected immunologically in the periplasm of a mutant that lacks all c-type cytochromes. a method for the preparation of apo-nitrite reductase (lacking both c- and d-type haem) from the holoenzyme of wild-type cells has been developed. the apoprotein synthesized by the mutant is indistinguishable from the chemically prepared apoprotein in respect of: (i) subunit molecular weight; (ii) formation of a homodimer; ... | 1989 | 2548064 |
| redox protein electron-transfer mechanisms: electrostatic interactions as a determinant of reaction site in c-type cytochromes. | the effect of ionic strength on the rate constant for electron transfer has been used to determine the magnitude and charge sign of the net electrostatic potential which exists in close proximity to the sites of electron transfer on various c-type cytochromes. the negatively charged ferricyanide ion preferentially reacts at the positively charged exposed heme edge region on the front side of horse cytochrome c and paracoccus cytochrome c2. in contrast, at low ionic strength, the positively charg ... | 1989 | 2551370 |
| deletion of the gene for subunit iii leads to defective assembly of bacterial cytochrome oxidase. | coiii is one of the major subunits in the mitochondrial and a bacterial cytochrome c oxidase, cytochrome aa3. it does not contain any of the enzyme's redox-active metal centres and can be removed from the enzyme without major changes in its established functions. we have deleted the coiii gene from paracoccus denitrificans. the mutant still expresses spectroscopically detectable enzyme almost as the wild-type, but its cytochrome c oxidase activity is much lower. from 50 to 80% of cytochrome a is ... | 1989 | 2555169 |
| transposable elements for efficient manipulation of a wide range of gram-negative bacteria: promoter probes and vectors for foreign genes. | we describe here the construction and use of a series of modified transposons based on the insertion sequence is1. like their parent, omegon-km [fellay et al., gene 76 (1989) 215-226], these elements permit efficient insertional mutagenesis of a variety of gram-negative bacteria. the presence of a functional pbr322 origin of replication within the transposable element facilitates subsequent cloning of the mutated gene. the omegon-km system was previously shown to function in pseudomonas putida, ... | 1989 | 2559879 |
| cloning and sequencing of the gene encoding the 72-kilodalton dehydrogenase subunit of alcohol dehydrogenase from acetobacter aceti. | a genomic library of acetobacter aceti dna was constructed by using a broad-host-range cosmid vector. complementation of a spontaneous alcohol dehydrogenase-deficient mutant resulted in the isolation of a plasmid designated paa701. subcloning and deletion analysis of paa701 limited the region that complemented the deficiency in alcohol dehydrogenase activity of the mutant. the nucleotide sequence of this region was determined and showed that this region contained the full structural gene for the ... | 1989 | 2722742 |
| steady-state kinetic analysis of the quinoprotein methylamine dehydrogenase from paracoccus denitrificans. | a steady-state kinetic analysis was performed of the reaction of methylamine and phenazine ethosulphate (pes) with the quinoprotein methylamine dehydrogenase from paracoccus denitrificans. experiments with methylamine and pes as varied-concentration substrates produced a series of parallel reciprocal plots, and when the concentrations of these substrates were varied in a constant ratio a linear reciprocal plot of initial velocity against pes concentration was obtained. nearly identical values of ... | 1989 | 2775197 |
| some characteristics of a chromophoric lipid associated with nitric oxide reductase from paracoccus denitrificans. | a lipid with a uv chromophore (lambda max = 274 nm) was purified in small amounts from nitric oxide reductase of paracoccus denitrificans and determined to have a molecular weight of 686, a most probable formula of c43h78o4n2 and about two phenylhydrazine-reactive carbonyls. on the basis of 1h and 13c-nmr, ir and mass spectrometric studies, the chromophoric lipid was inferred to have something like bilateral symmetry and to contain ketone-like carbonyls, alkene centers and at least three alkyl o ... | 1989 | 2803506 |
| cox10 codes for a protein homologous to the orf1 product of paracoccus denitrificans and is required for the synthesis of yeast cytochrome oxidase. | respiratory-defective mutants of saccharomyces cerevisiae assigned to pet complementation group g19 lack cytochrome oxidase activity and cytochromes a and a3. the enzyme deficiency is caused by recessive mutations in the nuclear gene cox10. analyses of cytochrome oxidase subunits suggest that the product of cox10 provides an essential function at a posttranslational stage of enzyme assembly. the wild type cox10 gene has been cloned by transformation of a mutant from complementation group g19 wit ... | 1990 | 2167310 |
| kinetics of oxidative phosphorylation in paracoccus denitrificans. 2. evidence for a kinetic and thermodynamic modulation of f0f1-atpase by the activity of the respiratory chain. | (1) the affinity of the f0f1-atpase from paracoccus denitrificans for atp during nadh-driven oxidative phosphorylation has been analyzed under different conditions by examining the type and extent of product inhibition. (2) a limited collapse of the protonmotive force (delta p) due to partial uncoupling does not increase the affinity for atp at the active site(s) of the enzyme; instead, a partial noncompetitive inhibition becomes apparent, compatible with the binding of atp to a noncatalytic sit ... | 1990 | 2148691 |
| kinetics of oxidative phosphorylation in paracoccus denitrificans. 1. mechanism of atp synthesis at the active site(s) of f0f1-atpase. | (1) the rate of atp synthesis during nadh-driven aerobic respiration has been measured in plasma membrane vesicles from paracoccus denitrificans as a function of the concentration of the substrates, adp and inorganic phosphate (pi). in both cases, the response of the reaction to changes in the degree of saturation of the f0f1-atpase generated a perfect micaelian dependence which allowed the determination of the corresponding michaelis constants, kmadp and kmpi. (2) these kinetic parameters posse ... | 1990 | 2148690 |
| the kinetic and isotopic competence of nitric oxide as an intermediate in denitrification. | rates of no uptake by five denitrifying bacteria were estimated by no-electrode and gas chromatography methods under conditions of rather low cell densities and [noaq]. the rates so measured, vmaxno, represent lower limits for the true value of that parameter, but nevertheless exceed vmax for nitrite uptake, vmaxni, by a factor of two typically. previous estimates under suboptimal conditions had placed vmaxno at 0.3-0.5 of vmaxni (st. john, r. t., and hollocher, t. c. (1977) j. biol. chem. 252, ... | 1990 | 2295624 |
| hydroxylamine as an inhibitor and terminal acceptor in the respiratory chain of the bacterium paracoccus denitrificans. | three sites of inhibitory action of hydroxylamine were identified in the respiratory chain of anaerobically grown bacterium paracoccus denitrificans. terminal oxidases were blocked at concentrations of 10(-4) to 10(-3) mol.l-1, and the inhibitor competed with artificial donor of electrons n, n, n', n'-tetramethyl-l, 4-phenylenediamine. in the anaerobic part of the respiratory chain inhibition of nitrite reductase and apparently also nitric oxide reductase occurred, resulting in the increased acc ... | 1990 | 2269422 |
| mutagenesis of the gene encoding amicyanin of paracoccus denitrificans and the resultant effect on methylamine oxidation. | the gene encoding the blue-copper protein amicyanin was isolated from a genomic bank of paracoccus denitrificans by using a synthetic oligonucleotide. it is located directly downstream of the gene encoding the small subunit of methylamine dehydrogenase. amicyanin is transcribed as a precursor protein with a signal sequence, typical for periplasmic proteins. specific inactivation of amicyanin by means of gene replacement techniques resulted in the complete loss of the ability to grow on methylami ... | 1990 | 2261991 |
| chemical cross-linking study of complex formation between methylamine dehydrogenase and amicyanin from paracoccus denitrificans. | two soluble periplasmic redox proteins from paracoccus denitrificans, the quinoprotein methylamine dehydrogenase and the copper protein amicyanin, form a weakly associated complex that is critical to their physiological function in electron transport [gray, k. a., davidson, v. l., & knaff, d. b. (1988) j. biol. chem. 263, 13987-13990]. the specific interactions between methylamine dehydrogenase and amicyanin have been studied by using the water-soluble cross-linking agent 1-ethyl-3-[3-(dimethyla ... | 1990 | 2383547 |
| inhibition by trimethylamine of methylamine oxidation by paracoccus denitrificans and bacterium w3a1. | trimethylamine, a common substrate for methylotrophic growth, specifically inhibited methylamine-dependent respiration by paracoccus denitrificans and bacterium w3a1. these effects were caused by the specific inhibition by trimethylamine of the periplasmic quinoprotein methylamine dehydrogenase. steady-state kinetic analysis of the effect of trimethylamine on methylamine oxidation by methylamine dehydrogenase indicated that the inhibition was a mixed type. apparent ki values for trimethylamine o ... | 1990 | 2331476 |
| ph-dependent semiquinone formation by methylamine dehydrogenase from paracoccus denitrificans. evidence for intermolecular electron transfer between quinone cofactors. | the quinonoid confactors of paracoccus denitrificans methylamine dehydrogenase exhibited a ph-dependent redistribution of electrons from the 50% reduced plus 50% oxidized to the 100% semiquinone redox form. this phenomenon was only observed at ph values greater than 7.5. the semiquinone was not readily reduced by addition of methylamine, consistent with the view that this substrate donates two electrons at a time to each cofactor during catalysis. once formed at ph 9.0, no change in redox state ... | 1990 | 2271681 |
| paracoccus aminophilus sp. nov. and paracoccus aminovorans sp. nov., which utilize n,n-dimethylformamide. | two methylamine- and n,n-dimethylformamide-utilizing paracoccus spp. are described. these bacteria are gram-negative, nonsporeforming, nonmotile, coccoid or short rod-shaped organisms. their dna base composition is 62 to 68 mol% g + c. their cellular fatty acids include large amounts of c18:1 acid. their major hydroxy acids are 3-oh c10:0 and 3-oh c14:0 acids. the major ubiquinone is q-10. these bacteria are distinguished from paracoccus denitrificans and paracoccus alcaliphilus by physiological ... | 1990 | 2397196 |
| paracoccus kocurii sp. nov., a tetramethylammonium-assimilating bacterium. | a new species of tetramethylammonium-assimilating bacteria was isolated from an activated sludge which was used for the treatment of tetramethylammonium hydroxide contained in the wastewater from semiconductor manufacturing processes. cells of the bacteria were gram-negative, nonmotile, short rods (0.5 to 0.8 micron by 0.7 to 1.1 microns). the major respiratory quinone component of the bacteria was q-10. the g + c content was 71 mol%. isolates are mesophilic and assimilate methylated amines such ... | 1990 | 2397197 |
| periplasmic and membrane-bound respiratory nitrate reductases in thiosphaera pantotropha. the periplasmic enzyme catalyzes the first step in aerobic denitrification. | the unusual ability of thiosphaera pantotropha to catalyze respiratory nitrate reduction under aerobic conditions is shown to correlate with the activity of a periplasmic nitrate reductase that is expressed under both aerobic and anaerobic growth conditions. the organism also synthesizes, but only under anaerobic conditions, a membrane-bound nitrate reductase which resembles the corresponding enzyme in paracoccus denitrificans in respect of both catalytic properties and inhibition of activity in ... | 1990 | 2365057 |
| steady-state nitric oxide concentrations during denitrification. | three species of denitrifying bacteria, paracoccus denitrificans, pseudomonas stutzeri strain jm300, and achromobacter cycloclastes, were allowed to reduce nitrate or nitrite in anaerobic, closed vials while the equilibration of gases between aqueous and gas phases was facilitated by vigorous stirring. the gas phase was sampled and analyzed for no with use of a chemiluminescence detector calibrated against bottled no standards or against no produced by the nitrite-iodide reaction. [noaq] was inf ... | 1990 | 2365685 |
| formation of a potent respiratory inhibitor at nitrite reduction by nitrite reductase isolated from the bacterium paracoccus denitrificans. | a new method of dissimilatory nitrite reductase (cytochrome cd1) isolation from the periplasmic fraction of anaerobically grown cells of the bacterium paracoccus denitrificans was developed, using ionex and gel permeation chromatography with fplc system (pharmacia, sweden). in experiments with isolated enzyme it was shown that through a nitrite reduction, catalysed by this enzyme, a substance (presumably nitric oxide) was formed which at submicromolar concentrations inhibited terminal cytochrome ... | 1990 | 2266492 |
| cytochrome oxidase assembly in yeast requires the product of cox11, a homolog of the p. denitrificans protein encoded by orf3. | the synthesis of cytochrome oxidase in saccharomyces cerevisiae was recently shown to require a protein encoded by the nuclear gene cox10. this protein was found to be homologous to the putative protein product of the open reading frame orf1 reported in one of the cytochrome oxidase operons of paracoccus denitrificans. in the present study we demonstrate the existence in yeast of a second nuclear gene, cox11, whose encoded protein is homologous to another open reading frame (orf3) present in the ... | 1990 | 2167832 |
| apo forms of cytochrome c550 and cytochrome cd1 are translocated to the periplasm of paracoccus denitrificans in the absence of haem incorporation caused either mutation or inhibition of haem synthesis. | an apo form of cytochrome c550 can be detected by immunoblotting cell-free extracts of a mutant of paracoccus denitrificans that is deficient in c-type cytochromes. this apoprotein is found predominantly in the periplasm, the location of the holocytochrome in the wild-type organism, indicating that translocation of the polypeptide occurs in the absence of haem attachment. the polypeptide molecular weight, as judged by sodium dodecyl sulphate/polyacrylamide gel electrophoresis, is indistinguishab ... | 1990 | 2172694 |
| the cellular location and specificity of bacterial cytochrome c peroxidases. | the locations of cytochrome c peroxidase and catalase activities in the two gram-negative bacteria pseudomonas stutzeri (n.c.i.b. 9721) and paracoccus denitrificans (n.c.i.b. 8944) were investigated by the production of spheroplasts. in both species the cytochrome c peroxidase was predominantly periplasmic: 92% of total activity in ps. stutzeri and 98% of nonmembrane-bound activity in pa. denitrificans were found in this cellular compartment. in contrast, the catalase was mostly in the cytoplasm ... | 1990 | 2173903 |
| kinetics of iron acquisition from ferric siderophores by paracoccus denitrificans. | the kinetics of iron accumulation by iron-starved paracoccus denitrificans during the first 2 min of exposure to 55fe-labeled ferric siderophore chelates is described. iron is acquired from the ferric chelate of the natural siderophore l-parabactin in a process exhibiting biphastic kinetics by lineweaver-burk analysis. the kinetic data for 1 microm less than [fe l-parabactin] less than 10 microm fit a regression line which suggests a low-affinity system (km = 3.9 +/- 1.2 microm, vmax = 494 pg-at ... | 1990 | 2185228 |
| nucleotide sequence of the methylobacterium extorquens am1 moxf and moxj genes involved in methanol oxidation. | the nucleotide sequence has been determined for two genes involved in methanol oxidation in the facultative methylotroph, methylobacterium extorquens am1. the two genes are moxf, encoding the 66-kda subunit of the methanol dehydrogenase and moxj, located immediately downstream from moxf, which encodes a 30-kda protein with unknown function. this information completes the sequence of the 5.86-kb xhoi-sali fragment containing the moxfjgi region in m. extorquens am1, and the structure of this gene ... | 1990 | 2116368 |
| identification of the nadh-binding subunit of nadh-ubiquinone oxidoreductase of paracoccus denitrificans. | the nadh dehydrogenase complex isolated from paracoccus denitrificans is composed of approximately 10 unlike polypeptides and contains noncovalently bound fmn, non-heme iron, and acid-labile sulfide [yagi, t. (1986) arch. biochem. biophys. 250, 302-311]. when the paracoccus nadh dehydrogenase complex was irradiated by uv light in the presence of [adenylate-32p]nad, radioactivity was incorporated exclusively into one of three polypeptides of mr approximately 50,000. similar results were obtained ... | 1990 | 2117469 |
| radiorespirometry evidence for the discrimination between 13c-enriched glucose and unlabelled glucose molecules by paracoccus denitrificans. | paracoccus denitrificans was grown on either unlabelled glucose, [1-13c]glucose or [6-13c]glucose as the sole carbon source for growth. the cells were then incubated with a range of 14c-glucose substrates to compare the 14co2-evolution rates between cells grown on the glucose and the 13c-labelled glucose. cells grown on 13c-glucose had significantly faster rates of 14co2-evolution than those grown on unlabelled glucose. the % yields of 14co2, per [1-14c]-, [6-14c]- and [u-14c]glucose supplied we ... | 1990 | 2117553 |
| inhibition by capsaicin of nadh-quinone oxidoreductases is correlated with the presence of energy-coupling site 1 in various organisms. | the nadh-ubiquinone reductase activity of the respiratory chains of several organisms was inhibited by capsaicin and dihydrocapsaicin, which are the pungent principles of red pepper. this inhibition was correlated with the presence of an energy transducing site in this segment of the respiratory chain. where the nadh-quinone oxidoreductase segment involved an energy coupling site (e.g., in paracoccus denitrificans, escherichia coli, and thermus thermophilus hb-8 membranes and bovine heart mitoch ... | 1990 | 2118334 |
| genetics of carbon metabolism in methylotrophic bacteria. | the application of genetic techniques to the methylotrophic bacteria has greatly enhanced studies of these important organisms. two methylotrophic systems have been studied in some detail, the serine cycle for formaldehyde assimilation and the methanol oxidation system. in both cases, genes have been cloned and mapped in methylobacterium species (facultative serine cycle methanol-utilizers). in addition, methanol oxidation genes have been studied in an autotrophic methanol-utilizer (paracoccus d ... | 1990 | 2128803 |
| mutagenesis of the gene encoding cytochrome c550 of paracoccus denitrificans and analysis of the resultant physiological effects. | by using synthetic oligonucleotides, the gene encoding soluble cytochrome c550 was isolated from a genomic bank of paracoccus denitrificans. the nucleotide sequence of the gene was determined, and the deduced amino acid sequence of the mature protein was found to be similar to the primary structure of purified cytochrome c550 except for the presence of seven additional amino acid residues at the c terminus. at the n terminus of the primary structure was found an additional stretch of 19 amino ac ... | 1990 | 2153663 |