Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| effects of vaginal brucella ovis infection of red deer hinds on reproductive performance, and venereal transmission to stags. | to investigate the effects of vaginal brucella ovis infection on the reproductive performance of red deer (cervus elaphus) hinds. to determine whether stags may become infected with b. ovis by venereal transmission from mating infected hinds. | 2002 | 16032258 |
| identification of species of brucella using fourier transform infrared spectroscopy. | fourier transform infrared spectroscopy (ftir) is a technique that has been used over the years in chemical analysis for the identification of substances and is one that may be applied to the characterisation of microorganisms. the marked tendency of brucella towards variation in the smooth rough phase, together with the laboriousness and risk involved in the methods used in their identification, make their classification difficult. we studied the type strains of the different species and biovar ... | 2003 | 14500003 |
| molecular characterization of brucella abortus chromosome ii recombination. | large-scale genomic rearrangements including inversions, deletions, and duplications are significant in bacterial evolution. the recently completed brucella melitensis 16m and brucella suis 1330 genomes have facilitated the investigation of such events in the brucella spp. suppressive subtractive hybridization (ssh) was employed in identifying genomic differences between b. melitensis 16m and brucella abortus 2308. analysis of 45 ssh clones revealed several deletions on chromosomes of b. abortus ... | 2003 | 14526025 |
| [evaluation of 2 hemoculture media for the isolation of brucella spp.]]. | the diagnostic efficiency of two hemoculture media for the detection of different species of brucella strains was evaluated. strains of brucella melitensis, brucella suis, brucella abortus, brucella ovis, and brucella abortus s19 were used. each strain was diluted in phosphate buffer saline (pbs) to obtain a concentration of 10(5) colony forming units/ml (cfu/ml). blood from goats, pigs, cattle, and sheep was mixed with the bacterial suspension to obtain a final concentration minor or equal to 1 ... | 2003 | 14587372 |
| identification and distribution of insertion sequences of paracoccus solventivorans. | three novel insertion sequences (iss) (ispso1, ispso2, and ispso3) of the soil bacterium paracoccus solventivorans dsm 11592 were identified by transposition into entrapment vector pmec1. ispso1 (1,400 bp) carries one large open reading frame (orf) encoding a putative basic protein (with a dde motif conserved among transposases [tnps] of elements belonging to the is256 family) with the highest levels of similarity with the hypothetical tnps of rhodospirillum rubrum and sphingopyxis macrogoltabid ... | 2003 | 14660342 |
| evaluation of a pcr test for the diagnosis of brucella ovis infection in semen samples from rams. | the sensitivity and specificity of a pcr assay with primers derived from the insertion sequence is6501 was compared with that of bacteriological culture and serological tests for the diagnosis of brucella ovis infection in rams. no amplifications were detected with dnas from the strains phylogenetically related to brucella and from the seven bacterial species considered as the main etiologic agents of epididymitis in rams. in addition, the specificity of the pcr was 100% when testing semen sampl ... | 2003 | 12488071 |
| serological diagnosis of brucellosis caused by brucella canis. | blood serum samples from 2,328 dogs were tested to detect antibodies against brucella canis with the agar gel immunodiffusion (agid) and 2-mercaptoethanol slide agglutination test (me-sat) using brucella ovis as the antigen. all blood serum samples were also evaluated for antibodies against brucella abortus and brucella melitensis using the rose bengal test. twentyfive (1.07%) of the sera evaluated were considered positive with agid test. only 4 (16%) of these blood serum samples were positive w ... | 2003 | 12578313 |
| production of the type iv secretion system differs among brucella species as revealed with virb5- and virb8-specific antisera. | expression of the virb operon, encoding the type iv secretion system required for brucella suis virulence, occurred in the acidic phagocytic vacuoles of macrophages and could be induced in minimal medium at acidic ph values. to analyze the production of virb proteins, polyclonal antisera against b. suis virb5 and virb8 were generated. western blot analysis revealed that virb5 and virb8 were detected after 3 h in acidic minimal medium and that the amounts increased after prolonged incubation. unl ... | 2003 | 12595417 |
| role of the brucella suis lipopolysaccharide o antigen in phagosomal genesis and in inhibition of phagosome-lysosome fusion in murine macrophages. | brucella species are gram-negative, facultative intracellular bacteria that infect humans and animals. these organisms can survive and replicate within a membrane-bound compartment inside professional and nonprofessional phagocytic cells. inhibition of phagosome-lysosome fusion has been proposed as a mechanism for intracellular survival in both cell types. however, the molecular mechanisms and the microbial factors involved are poorly understood. smooth lipopolysaccharide (lps) of brucella has b ... | 2003 | 12595466 |
| the recombinant omp31 from brucella melitensis alone or associated with rough lipopolysaccharide induces protection against brucella ovis infection in balb/c mice. | immunogenicity and protective activity against brucella ovis of detergent-extracted recombinant omp31 (romp31 extract) from brucella melitensis produced in escherichia coli, purified rough lipopolysaccharide from b. ovis (r-lps) and a mixture of romp31 extract and r-lps (romp31 extract + r-lps) were assessed in balb/c mice. the experimental vaccines were compared with a hot saline extract (hs extract) from b. ovis mainly composed of outer membrane proteins (omps) and r-lps, and known to be prote ... | 2003 | 12650766 |
| type iii secretion homologs are present in brucella melitensis, b. ovis, and b. suis biovars 1, 2, and 3. | protein sequences from characterized type iii secretion (tts) systems were used as probes in silico to identify several tts gene homologs in the genome sequence of brucella suis biovar 1 strain 1330. four of the genes, named flhb, flip, flir, and flif on the basis of greatest homologies to known flagellar apparatus proteins, were targeted in pcr and hybridization assays to determine their distribution among other brucella nomen species and biovars. the results indicated that flhb, flip, flir and ... | 2003 | 12732970 |
| characterization of brucella abortus o-polysaccharide and core lipopolysaccharide mutants and demonstration that a complete core is required for rough vaccines to be efficient against brucella abortus and brucella ovis in the mouse model. | brucella abortus rough lipopolysaccharide (lps) mutants were obtained by transposon insertion into two wbk genes (wbka [putative glycosyltransferase; formerly rfbu] and per [perosamine synthetase]), into manb (pmm [phosphomannomutase; formerly rfbk]), and into an unassigned gene. consistent with gene-predicted roles, electrophoretic analysis, 2-keto-3-manno-d-octulosonate measurements, and immunoblots with monoclonal antibodies to o-polysaccharide, outer and inner core epitopes showed no o-polys ... | 2003 | 12761107 |
| association of maedi visna virus with brucella ovis infection in rams. | maedi visna virus (mvv) is the etiological agent of a systemic disease of sheep, which causes lesions in lungs, the central nervous system, joints, and mammary glands. it has been speculated that the association with brucella ovis may lead to the venereal shedding of the virus. in this work, samples of epididymis from ten rams positive for mvv and infected experimentally with brucella ovis, were subjected to liquid-phase pcr, immunohistochemistry (ihc) and in situ pcr tests, aimed at identifying ... | 2003 | 12777212 |
| identification and characterization of transposable elements of paracoccus pantotrophus. | we studied diversity and distribution of transposable elements residing in different strains (dsm 11072, dsm 11073, dsm 65, and lmd 82.5) of a soil bacterium paracoccus pantotrophus (alpha-proteobacteria). with application of a shuttle entrapment vector pmec1, several novel insertion sequences (iss) and transposons (tns) have been identified. they were sequenced and subjected to detailed comparative analysis, which allowed their characterization (i.e., identification of transposase genes, termin ... | 2003 | 12813068 |
| epitope mapping of the brucella melitensis bp26 immunogenic protein: usefulness for diagnosis of sheep brucellosis. | sequencing of bp26, the gene encoding the brucella sp. immunogenic bp26 periplasmic protein, was performed in the reference strains of brucella abortus, b. suis, and b. ovis. the three bp26 sequences were almost identical to that published for b. melitensis 16m bp26, and only minor nucleotide substitutions, without modifying the amino acid sequence, were observed between species. the bp26 genes of the seven b. abortus biovar reference strains and b. abortus s19 and rb51 vaccine strains were also ... | 2003 | 12853399 |
| brucella 'hoof-prints': strain typing by multi-locus analysis of variable number tandem repeats (vntrs). | currently, there are very few tools available for subtyping brucella isolates for epidemiological trace-back. subtyping is difficult because of the genetic homogeneity within the genus. sequencing of the genomes from three brucella species has facilitated the search for dna sequence variability. recently, hypervariability among short tandem repeat sequences has been exploited for strain-typing of several bacterial pathogens. | 2003 | 12857351 |
| brucella abortus rough mutants are cytopathic for macrophages in culture. | rough mutants of brucella spp. are attenuated for survival in animal models. however, conflicting in vitro evidence has been obtained concerning the intracellular survival of rough mutants. transposon-derived rough mutants isolated in our laboratory were previously shown to exhibit small but significant reductions in intracellular survival in a 12-h in vitro assay. several recent publications report that rough mutants exhibited increased macrophage uptake relative to their smooth parental strain ... | 2004 | 14688125 |
| antibody reactivity to omp31 from brucella melitensis in human and animal infections by smooth and rough brucellae. | group 3 of outer membrane proteins (omps) of brucella includes omp25 and omp31, which share 34% identity. omp25 is highly conserved in brucella species, and omp31 is present in all brucella species, except brucella abortus. antibodies to brucella melitensis omp31 have been sought only in infected sheep, and western blotting of sera from infected sheep did not reveal anti-omp31 reactivity. we obtained recombinant purified omp31 (b. melitensis) and tested its recognition by sera from humans and an ... | 2004 | 14715555 |
| bartholin's gland abscess caused by brucella melitensis. | we report herein a case of bartholin's gland abscess caused by brucella melitensis. clinical microbiology laboratory workers in areas where this disease is endemic should be familiar with the bacteriological features of this organism and consider the possibility of a brucellar etiology in a broad range of clinical settings. | 2004 | 14766890 |
| influence of the co-encapsulation of different excipients on the properties of polyester microparticle-based vaccine against brucellosis. | this work evaluates the influence of different pharmaceutical auxiliaries (pluronic f68, polyvinylpyrrolidone [pvp] or tween 20), when mixed with an antigenic extract from brucella ovis (hot saline; hs), on the characteristics of the resulting poly(epsilon-caprolactone) (pec) and poly(lactide-co-glycolide) (plga) microparticles. in all cases, pec microparticles were smaller than plga ones. concerning the hs loading, plga microparticles were highly dependent on the type of the excipient used, whe ... | 2004 | 15129979 |
| lack of evidence of brucella ovis infection in rams in quebec. | a study was conducted to estimate the seroprevalence of brucella ovis infection in rams in the estrie and bas-saint-laurent regions (quebec). rams sera (n = 258) were serologically evaluated from 224 rams in 30 commercial flocks and from 34 rams at 2 slaughterhouses by using an enzyme linked immunosorbent assay. epididymides and testes were examined by palpation on farms and microscopically for culled rams. no ram was seropositive to brucella ovis or had lesions suggestive of brucellosis from th ... | 2004 | 15144103 |
| development and evaluation as vaccines in mice of brucella melitensis rev.1 single and double deletion mutants of the bp26 and omp31 genes coding for antigens of diagnostic significance in ovine brucellosis. | the live attenuated brucella melitensis rev.1 strain is considered the best vaccine available for the prophylaxis of brucellosis in sheep caused by either b. melitensis or brucella ovis. however, its application stimulates antibody responses in vaccinated animals indistinguishable by the current conventional serological tests from those observed in infected animals. the periplasmic protein bp26 and the outer membrane protein (omp) omp31 are immunodominant antigens in the serological responses of ... | 2004 | 15246618 |
| occurrence and potential diagnostic applications of serological cross-reactivities between brucella and other alpha-proteobacteria. | agrobacterium, sinorhizobium, and ochrobactrum are genera closely related to brucella but, in contrast to the latter, are not pathogenic for humans and animals. we studied by an indirect enzyme-linked immunosorbent assay (elisa) the reactivities of brucellosis sera against cytosolic (cyt) and membrane (ma) antigens from these nonpathogenic bacteria, and we evaluated the potential usefulness of these cross-reactions for the diagnosis of brucellosis in humans, sheep, cows, and dogs. canine infecti ... | 2004 | 15358645 |
| dna polymorphism in the omp25/omp31 family of brucella spp.: identification of a 1.7-kb inversion in brucella cetaceae and of a 15.1-kb genomic island, absent from brucella ovis, related to the synthesis of smooth lipopolysaccharide. | five genes homologous to the well-known omp25 and omp31 genes, that code for two major brucella spp. outer membrane proteins (omps), have been detected in the genome of brucella melitensis 16m and brucella suis 1330. in this work we have determined the nucleotide sequence of these five genes, named omp31b, omp25b, omp25c, omp25d and omp22, in the six classical brucella species reference strains and in representative strains of the recently proposed species brucella cetaceae and brucella pinniped ... | 2004 | 15374004 |
| genetic bases of the rifampin resistance phenotype in brucella spp. | rifampin is one of the most potent and broad-spectrum antibiotics against bacterial pathogens. its bactericidal activity is due to its ability to bind to the beta subunit of the dna-dependent rna polymerase encoded by the rpob gene. mutations of the rpob gene have been characterized in rifampin-resistant (rif(r)) strains of escherichia coli and mycobacterium tuberculosis. the genetic bases of rif(r) in brucella spp. are still unknown. in the present study, the nucleotide sequences of the rpob ge ... | 2004 | 15583262 |
| atp-binding cassette transporters are targets for the development of antibacterial vaccines and therapies. | 2004 | 15557595 | |
| use of brucella canis antigen for detection of ovine serum antibodies against brucella ovis. | brucella ovis causes a genital disease of sheep manifested by epididymitis in rams and placentitis in ewes producing reduced fertility in the flock. clinical diagnosis is not sensitive enough and bacteriological testing is not feasible for detection of the disease in large numbers of animals. indirect methods of serological testing are preferred for routine diagnosis, of which agar gel immunodiffusion (agid), complement fixation (cf) and elisa tests are recommended as the most efficient. since b ... | 2004 | 15708814 |
| subcellular fractions of brucella ovis distinctively induce the production of interleukin-2, interleukin-4, and interferon-gamma in mice. | the aim of this study was to evaluate the effect of 3 brucella ovis subcellular protein fractions: outer membrane (omp), inner membrane (imp), and cytoplasm (cp), on cellular immune response by in vitro production of interleukin (il)-2, il-4, and interferon (ifn)-gamma. each fraction was inoculated 3 times into balb/c mice, primary cultures of mice spleen cells were done, and these were then stimulated with the fractions. culture supernatants were collected at 24, 48, 72, 96, and 120 h postinocu ... | 2005 | 15745223 |
| rhizobial 16s rrna and dnak genes: mosaicism and the uncertain phylogenetic placement of rhizobium galegae. | the phylogenetic relatedness among 12 agriculturally important species in the order rhizobiales was estimated by comparative 16s rrna and dnak sequence analyses. two groups of related species were identified by neighbor-joining and maximum-parsimony analysis. one group consisted of mesorhizobium loti and mesorhizobium ciceri, and the other group consisted of agrobacterium rhizogenes, rhizobium tropici, rhizobium etli, and rhizobium leguminosarum. although bootstrap support for the placement of t ... | 2005 | 15746335 |
| restriction site polymorphisms in the genes encoding new members of group 3 outer membrane protein family of brucella spp. | thirty-seven brucella reference and field strains representing all the species and their biovars were analysed by pcr-rflp to determine the degree of variation in the genes encoding the new members of group 3 outer membrane protein (omp) family. analysis of the omp22 and omp25c/omp25d genes indicated that the restriction patterns were identical for all species and biovars with all restriction enzymes tested, except for brucella ovis that showed a short 30 bp deletion close to omp22 gene, and for ... | 2005 | 15796983 |
| demonstration of polymorphism among brucella ovis field isolates by pulsed-field gel electrophoresis. | brucella ovis is recognized worldwide as an important pathogen of sheep, and has also been identified in farmed deer in new zealand. previously, only one strain type of b. ovis has been identified. the objective of this paper was to perform pulsed-field gel electrophoresis (pfge) on field isolates of b. ovis to determine whether strain variations exist, whether sheep and deer are affected by the same strains, and to compare the performance of the rare-cutting restriction enzymes xbai and swai. t ... | 2005 | 15871911 |
| evaluation of the sprague-dawley rat as a model for vertical transmission of brucella abortus. | vertical transmission of brucella abortus in sprague-dawley (sd) rats was verified with microbiologic, serologic, and polymerase chain reaction (pcr) methods. the 38 initially brucella-free sd rats, weighing 200 to 250 g, were injected subcutaneously with 50 microl of a suspension containing 1 x 10(9) colony-forming units (cfu) of b. abortus biotype 1 korean isolate. the rats were allowed to mate with uninfected sd rats. the isolate was detected by culture and by amos (abortus, melitensis, ovis, ... | 2005 | 16479730 |
| efficacy of several serological tests and antigens for diagnosis of bovine brucellosis in the presence of false-positive serological results due to yersinia enterocolitica o:9. | yersinia enterocolitica o:9 bears a smooth lipopolysaccharide (s-lps) of brucella sp. o-chain a+c/y epitopic structure and is a cause of false-positive serological reactions (fpsr) in standard tests for cattle brucellosis. brucella s-lps, cross-reacting s-lpss representing several o-chain epitope combinations, brucella core lipid a epitopes (rough lps), brucella abortus s-lps-derived polysaccharide, native hapten polysaccharide, rough lps group 3 outer membrane protein complexes, recombinant bp2 ... | 2005 | 15642999 |
| characterization of the genome composition of bartonella koehlerae by microarray comparative genomic hybridization profiling. | bartonella henselae is present in a wide range of wild and domestic feline hosts and causes cat-scratch disease and bacillary angiomatosis in humans. we have estimated here the gene content of bartonella koehlerae, a novel species isolated from cats that was recently identified as an agent of human endocarditis. the investigation was accomplished by comparative genomic hybridization (cgh) to a microarray constructed from the sequenced 1.93-mb genome of b. henselae. control hybridizations of labe ... | 2005 | 16109957 |
| whole-genome analyses of speciation events in pathogenic brucellae. | despite their high dna identity and a proposal to group classical brucella species as biovars of brucella melitensis, the commonly recognized brucella species can be distinguished by distinct biochemical and fatty acid characters, as well as by a marked host range (e.g., brucella suis for swine, b. melitensis for sheep and goats, and brucella abortus for cattle). here we present the genome of b. abortus 2308, the virulent prototype biovar 1 strain, and its comparison to the two other human patho ... | 2005 | 16299333 |
| brucella outer membrane complex-loaded microparticles as a vaccine against brucella ovis in rams. | due to the important drawbacks of the brucella melitensis rev 1 vaccine, a safer vaccine based on an outer membrane complex from brucella ovis encapsulated in poly-epsilon-caprolactone (pec) microparticles (mp) was developed and tested in rams. homogeneous batches of microparticles were prepared by a new double emulsion solvent evaporation method called "total recirculation one-machine system" (troms). such microparticles presented a mean diameter of 2 microm and displayed an antigen loading of ... | 2006 | 16337315 |
| highly sensitive real-time pcr for specific detection and quantification of coxiella burnetii. | coxiella burnetii, the bacterium causing q fever, is an obligate intracellular biosafety level 3 agent. detection and quantification of these bacteria with conventional methods is time consuming and dangerous. during the last years, several pcr based diagnostic assays were developed to detect c. burnetii dna in cell cultures and clinical samples. we developed and evaluated taqman-based real-time pcr assays that targeted the singular icd (isocitrate dehydrogenase) gene and the transposase of the ... | 2006 | 16423303 |
| experiments on a sub-unit vaccine encapsulated in microparticles and its efficacy against brucella melitensis in mice. | the aim of this study was to evaluate the effect of the excipients used to facilitate the encapsulation of high hydrophobic antigenic complex extracted from brucella ovis (hs) on the physico-chemical properties of the resulting microparticles. poly(epsilon-caprolactone) (pec) microparticles containing hs were prepared by the solvent extraction/evaporation method using total recirculation one-machine system (troms). different excipients, beta-cyclodextrin (beta-cd), pluronic f68, tween 20 or twee ... | 2006 | 16481077 |
| brucella outer membrane protein omp31 is a haemin-binding protein. | the expression of haemin-binding proteins (hbps) in the outer membrane is one of the strategies used by gram-negative bacteria to obtain iron from the host. no hbp has been described in brucella spp. we investigated whether omp31, an outer membrane protein from brucella with homology to hbps from bartonella quintana, is an hbp. soluble recombinant omp31 bound specifically to haemin-agarose, while an unrelated brucella protein (sura) did not. a similar experiment showed that native omp31 found in ... | 2006 | 16517201 |
| identification of an immunogenic protein of actinobacillus seminis that is present in microvesicles. | actinobacillus seminis is a gram-negative bacterium of the pasteurellaceae family that is involved in ovine epididymitis. looking for a protein specific to this species, we determined the protein profile of subcellular fractions of a. seminis (american type culture collection number 15768): proteins from the outer membrane (omps), inner membrane (imps), and cytoplasm (cps). these profiles provide the first data, to our knowledge, regarding subcellular fractions of a. seminis. in the omp fraction ... | 2006 | 16548331 |
| brucella spp noncanonical lps: structure, biosynthesis, and interaction with host immune system. | brucella spp. are facultative intracellular pathogens that have the ability to survive and multiply in professional and non-professional phagocytes, and cause abortion in domestic animals and undulant fever in humans. several species are recognized within the genus brucella and this classification is mainly based on the difference in pathogenicity and in host preference. brucella strains may occur as either smooth or rough, expressing smooth lps (s-lps) or rough lps (r-lps) as major surface anti ... | 2006 | 16556309 |
| persistence, serodiagnosis and effects on semen characteristics of artificial brucella ovis infection in red deer stags. | to investigate the persistence of infection and serum antibody titres after infection of red deer (cervus elaphus) stags with brucella ovis, and compare these with those of rams. to assess the effects of recent and chronic infection on semen characteristics of stags. | 2006 | 16596160 |
| protective immunity elicited by a divalent dna vaccine encoding both the l7/l12 and omp16 genes of brucella abortus in balb/c mice. | this study was designed to evaluate the immunogenicity and the protective efficacy of a divalent fusion dna vaccine encoding both the brucella abortus l7/l12 protein (ribosomal protein) and omp16 protein (outer membrane lipoprotein), designated pcdna3.1-l7/l12-omp16. intramuscular injection of this divalent dna vaccine into balb/c mice elicited markedly both humoral and cellular immune responses. the specific antibodies exhibited a dominance of immunoglobulin g2a (igg2a) over igg1. in addition, ... | 2006 | 16622210 |
| cghscan: finding variable regions using high-density microarray comparative genomic hybridization data. | comparative genomic hybridization can rapidly identify chromosomal regions that vary between organisms and tissues. this technique has been applied to detecting differences between normal and cancerous tissues in eukaryotes as well as genomic variability in microbial strains and species. the density of oligonucleotide probes available on current microarray platforms is particularly well-suited for comparisons of organisms with smaller genomes like bacteria and yeast where an entire genome can be ... | 2006 | 16638145 |
| rapid detection of brucella spp. in blood cultures by fluorescence in situ hybridization. | brucellosis is a severe systemic disease in humans. we describe a new 16s rrna-based fluorescence in situ hybridization assay that facilitates rapid and specific detection of all human pathogenic species of brucella and that can be applied directly to positive blood cultures. | 2006 | 16672413 |
| detection of antibodies to brucella ovis in sheep milk using b. ovis and b. canis antigen. | the diagnostic techniques most widely used for detecting brucellosis caused by brucella ovis are serological tests such as complement fixation (cft), agar gel immunodiffusion (agid), and elisas. however, to our knowledge, milk tests, with the advantage that samples may be taken in a non invasive manner, have not been investigated as diagnostic tools. we studied 144 samples of milk and sera from lactating ewes, comparing bacteriological studies, serological and milk tests using brucella canis and ... | 2006 | 16678362 |
| optimization of the entrapment of bacterial cell envelope extracts into microparticles for vaccine delivery. | the encapsulation of a brucella ovis extract (hs) in microparticles has been proved effective against experimental infections in mice. this work describes different strategies to increase the hs loading and prepare large batches as necessary to test this vaccine in ovine. the mixture of hs with beta-cyclodextrin was optimized in order to increase the hs loading in microparticles. on the other hand, troms ('total recirculation one-machine system') led microparticles with a more homogeneous size t ... | 2006 | 16754373 |
| cloning, characterization, and expression of bartonella henselae p26. | in order to identify immunoreactive bartonella henselae proteins, b. henselae antiserum from an experimentally infected cat was used to screen a b. henselae genomic dna expression library. one immunoreactive phage clone contained a gene (p26) with significant nucleotide identity with orthologs in brucellae, bartonellae, and several plant-associated bacteria. p26 gene sequences from four b. henselae strains, one b. koehlerae strain, and one b. clarridgeiae strain were cloned. comparative nucleoti ... | 2006 | 16893981 |
| intrinsic and selected resistance to antibiotics binding the ribosome: analyses of brucella 23s rrn, l4, l22, ef-tu1, ef-tu2, efflux and phylogenetic implications. | brucella spp. are highly similar, having identical 16s rna. however, they have important phenotypic differences such as differential susceptibility to antibiotics binding the ribosome. neither the differential susceptibility nor its basis has been rigorously studied. differences found among other conserved ribosomal loci could further define the relationships among the classical brucella spp. | 2006 | 17014718 |
| cultivation of denitrifying bacteria: optimization of isolation conditions and diversity study. | an evolutionary algorithm was applied to study the complex interactions between medium parameters and their effects on the isolation of denitrifying bacteria, both in number and in diversity. growth media with a ph of 7 and a nitrogen concentration of 3 mm, supplemented with 1 ml of vitamin solution but not with sodium chloride or riboflavin, were the most successful for the isolation of denitrifiers from activated sludge. the use of ethanol or succinate as a carbon source and a molar c/n ratio ... | 2006 | 16597968 |
| genomics against flatulence. | 2007 | 17635535 | |
| multiplex pcr for the detection of brucella ovis, actinobacillus seminis and histophilus somni in ram semen. | to develop a multiplex polymerase chain reaction (pcr) assay for the rapid detection of brucella ovis, actinobacillus seminis, histophilus somni in fresh ram semen samples. | 2007 | 17300467 |
| phylogenomics and signature proteins for the alpha proteobacteria and its main groups. | alpha proteobacteria are one of the largest and most extensively studied groups within bacteria. however, for these bacteria as a whole and for all of its major subgroups (viz. rhizobiales, rhodobacterales, rhodospirillales, rickettsiales, sphingomonadales and caulobacterales), very few or no distinctive molecular or biochemical characteristics are known. | 2007 | 18045498 |
| evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting gram-negative rods. | to evaluate the activity of pyrrolidonyl arylamidase (pyr) for the differentiation and identification of nonfermenting gram negative rods (nfgnr), 293 isolates were tested. a 24 h culture of each test organism was prepared. from this a 108-109 cfu/ml suspension was added to 0.25 ml of sterile physiologic solution. a pyr disk was then added and the test was incubated for 30 minutes at 35-37 degrees c, at environmental atmosphere. reading was done by adding 1 drop of cinnamaldehyde reagent. strain ... | 2007 | 16822636 |
| improved immunogenicity of a vaccination regimen combining a dna vaccine encoding brucella melitensis outer membrane protein 31 (omp31) and recombinant omp31 boosting. | in the present study, we report an attempt to improve the immunogenicity of the omp31 antigen by a dna prime-protein boost immunization regimen. we immunized balb/c mice with an omp31 dna vaccine (pciomp31) followed by boosting with recombinant omp31 (romp31) in incomplete freund's adjuvant and characterized the resulting immune responses and the protective efficacy against brucella ovis and b. melitensis infection. immunoglobulin g1 (igg1) and igg2a titers were higher in sera from pciomp31/romp ... | 2007 | 17428946 |
| a recombinant subunit vaccine based on the insertion of 27 amino acids from omp31 to the n-terminus of bls induced a similar degree of protection against b. ovis than rev.1 vaccination. | the development of an effective subunit vaccine against brucellosis is a research area of intense interest. the enzyme lumazine synthase from brucella spp. (bls) is highly immunogenic, presumably due to its decameric arrangement and remarkable stability. in this work we decided to develop a chimera with the scaffold protein bls decorated with 10 copies of a known protective epitope derived from an outer membrane protein of 31kda (omp31) from brucella spp. vaccination of balb/c mice with the chim ... | 2007 | 17442465 |
| characterisation of the genetic diversity of brucella by multilocus sequencing. | brucella species include economically important zoonotic pathogens that can infect a wide range of animals. there are currently six classically recognised species of brucella although, as yet unnamed, isolates from various marine mammal species have been reported. in order to investigate genetic relationships within the group and identify potential diagnostic markers we have sequenced multiple genetic loci from a large sample of brucella isolates representing the known diversity of the genus. | 2007 | 17448232 |
| role of the omp25/omp31 family in outer membrane properties and virulence of brucella ovis. | the genes coding for the five outer membrane proteins (omps) of the omp25/omp31 family expected to be located in the outer membrane (om) of rough virulent brucella ovis pa were inactivated to evaluate their role in virulence and om properties. the om properties of the mutant strains and of the mutants complemented with the corresponding wild-type genes were analyzed, in comparison with the parental strain and rough b. abortus rb51, in several tests: (i) binding of anti-omp25 and anti-omp31 monoc ... | 2007 | 17562767 |
| gantrez an nanoparticles as an adjuvant for oral immunotherapy with allergens. | the aim of this study was to investigate the adjuvant properties of oral-administered gantrez an nanoparticles with ovalbumin (as allergen model) and, in some cases, lipopolysaccharide of brucella ovis as immunomodulator. for this purpose, balb/c mice were administered by oral gavage with ova nanoparticles and both th1 and th2 markers (igg2a and igg1, respectively) were enhanced. on the other hand, these carriers administered by oral route were able to protect a model of sensitized mice to ovalb ... | 2007 | 17576025 |
| a dna vaccine coding for the chimera blsomp31 induced a better degree of protection against b. ovis and a similar degree of protection against b. melitensis than rev.1 vaccination. | in the present study, we reported an attempt to improve the immunogenicity and protective capacity of the chimera blsomp31 using a different antigen delivery: dna vaccination. vaccination of balb/c mice with the dna vaccine coding for the chimera blsomp31 (pciblsomp31) provided the best protection level against brucella ovis, which was significantly higher than the given by the co-delivery of both plasmids coding for the whole proteins (pcdnabls+pciomp31) and even higher than the control vaccine ... | 2007 | 17600596 |
| detection of ovine antibody to brucella ovis by indirect enzyme immunoassay. | because some batch-to-batch variation in the preparation of rough lipopolysaccharide (rlps) from brucella ovis has been experienced, several protocols were tested to establish the most reliable method for detection of antibody in indirect enzyme immunoassay. an early version of the assay gave a performance index (pi=sum of optimum percent sensitivity and percent specificity, determined by receiver operator characteristic analysis) of 198.6. this assay used rlps from b. ovis as the antigen and a ... | 2007 | 17613670 |
| brucella abortus uses a stealthy strategy to avoid activation of the innate immune system during the onset of infection. | to unravel the strategy by which brucella abortus establishes chronic infections, we explored its early interaction with innate immunity. | 2007 | 17637846 |
| bvrr/bvrs-controlled outer membrane proteins omp3a and omp3b are not essential for brucella abortus virulence. | the brucella abortus two-component regulatory system bvrr/bvrs controls the expression of outer membrane proteins (omp) omp3a (omp25) and omp3b (omp22). disruption of bvrs or bvrr generates avirulent mutants with altered cell permeability, higher sensitivity to microbicidal peptides, and complement. consequently, the role of omp3a and omp3b in virulence was examined. similar to bvrs or bvrr mutants, omp3a and omp3b mutants displayed increased attachment to cells, indicating surface alterations. ... | 2007 | 17664262 |
| cghsweep: an algorithm for analyzing chromosomal aberrations in genome using acgh profiles. | dna copy number aberrations along the genome are vital markers for studying pathogenesis of various diseases including cancers. array-based comparative genome hybridization (acgh), which is a high-throughput cytogenetic method, helps in identifying genome-wide copy number aberrations, both gains and losses. here, we propose a computational technique to analyze acgh data and to identify potential dna copy number alterations along the genome. our technique detects the possible breakpoints by compa ... | 2007 | 18467776 |
| rapid identification of brucella isolates to the species level by real time pcr based single nucleotide polymorphism (snp) analysis. | brucellosis, caused by members of the genus brucella, remains one of the world's major zoonotic diseases. six species have classically been recognised within the family brucella largely based on a combination of classical microbiology and host specificity, although more recently additional isolations of novel brucella have been reported from various marine mammals and voles. classical identification to species level is based on a biotyping approach that is lengthy, requires extensive and hazardo ... | 2008 | 18518958 |
| allergen immunotherapy with nanoparticles containing lipopolysaccharide from brucella ovis. | the adjuvant and protective capacity against anaphylactic shock of the association between rough lipopolysaccharide of brucella ovis (lps) coencapsulated with ovalbumin (ova), as a model allergen, in gantrez an nanoparticles was investigated. several strategies were performed in order to study the adjuvant effect of the lps either encapsulated or coating the nanoparticles. ova, as well as lps, was incorporated either during the manufacturing process (ova-encapsulated or lps-encapsulated nanopart ... | 2008 | 18582571 |
| a bioinformatic approach to understanding antibiotic resistance in intracellular bacteria through whole genome analysis. | intracellular bacteria survive within eukaryotic host cells and are difficult to kill with certain antibiotics. as a result, antibiotic resistance in intracellular bacteria is becoming commonplace in healthcare institutions. owing to the lack of methods available for transforming these bacteria, we evaluated the mechanisms of resistance using molecular methods and in silico genome analysis. the objective of this review was to understand the molecular mechanisms of antibiotic resistance through i ... | 2008 | 18619818 |
| brucellosis vaccines: assessment of brucella melitensis lipopolysaccharide rough mutants defective in core and o-polysaccharide synthesis and export. | the brucellae are facultative intracellular bacteria that cause brucellosis, one of the major neglected zoonoses. in endemic areas, vaccination is the only effective way to control this disease. brucella melitensis rev 1 is a vaccine effective against the brucellosis of sheep and goat caused by b. melitensis, the commonest source of human infection. however, rev 1 carries a smooth lipopolysaccharide with an o-polysaccharide that elicits antibodies interfering in serodiagnosis, a major problem in ... | 2008 | 18648644 |
| rnai screen of endoplasmic reticulum-associated host factors reveals a role for ire1alpha in supporting brucella replication. | brucella species are facultative intracellular bacterial pathogens that cause brucellosis, a global zoonosis of profound importance. although recent studies have demonstrated that brucella spp. replicate within an intracellular compartment that contains endoplasmic reticulum (er) resident proteins, the molecular mechanisms by which the pathogen secures this replicative niche remain obscure. here, we address this issue by exploiting drosophila s2 cells and rna interference (rnai) technology to de ... | 2008 | 18654626 |
| prediction of sinorhizobium meliloti srna genes and experimental detection in strain 2011. | small non-coding rnas (srnas) have emerged as ubiquitous regulatory elements in bacteria and other life domains. however, few srnas have been identified outside several well-studied species of gamma-proteobacteria and thus relatively little is known about the role of rna-mediated regulation in most other bacterial genera. here we have conducted a computational prediction of putative srna genes in intergenic regions (igrs) of the symbiotic alpha-proteobacterium s. meliloti 1021 and experimentally ... | 2008 | 18793445 |
| incidence of brucella species in marine mammals of the german north sea. | in this study, organ samples from 426 common seals phoca vitulina, 298 harbour porpoises phocoena phocoena, 34 grey seals halichoerus grypus and 10 other marine mammals were assessed for the presence of brucella species. forty-seven common seals, 2 harbour porpoises and 1 grey seal were found to be positive for these bacteria. a total of 91 brucella strains were successfully isolated, due to the fact that brucella spp. were found in more than one organ sample in 15 animals. the primary organ in ... | 2008 | 18828563 |
| stability of poly(epsilon-caprolactone) microparticles containing brucella ovis antigens as a vaccine delivery system against brucellosis. | in previous works, our research group has successfully proved the use of subcellular vaccines based on poly(epsilon-caprolactone) (pec) microparticles containing an antigenic extract of brucella ovis (hs) against experimental brucellosis in both mice and rams. however, the successful exploitation of pharmaceutical products, and therefore of this product as veterinary vaccine, requires preservation of both biological activity and native structure in all steps of development from purification to s ... | 2008 | 18923907 |
| phenotypic and molecular characterisation of brucella isolates from marine mammals. | bacteria of the genus brucella are the causative organisms of brucellosis in animals and man. previous characterisation of brucella strains originating from marine mammals showed them to be distinct from the terrestrial species and likely to comprise one or more new taxa. recently two new species comprising brucella isolates from marine mammals, b. pinnipedialis and b. ceti, were validly published. here we report on an extensive study of the molecular and phenotypic characteristics of marine mam ... | 2008 | 19091076 |
| novel brucella strain (bo1) associated with a prosthetic breast implant infection. | we report the microbiological, biochemical, and molecular characterization of an unusual brucella strain (bo1) isolated from a breast implant wound in a 71-year-old woman with clinical symptoms consistent with brucellosis. initial phenotypic analysis, including biochemical and antimicrobial susceptibility testing, cellular fatty acid analysis, and molecular analysis based on dna-dna reassociation and the presence of multiple copies of is711 element suggested that the isolate was a brucella-like ... | 2008 | 17977982 |
| novel brucella strain (bo1) associated with a prosthetic breast implant infection. | we report the microbiological, biochemical, and molecular characterization of an unusual brucella strain (bo1) isolated from a breast implant wound in a 71-year-old woman with clinical symptoms consistent with brucellosis. initial phenotypic analysis, including biochemical and antimicrobial susceptibility testing, cellular fatty acid analysis, and molecular analysis based on dna-dna reassociation and the presence of multiple copies of is711 element suggested that the isolate was a brucella-like ... | 2008 | 17977982 |
| brucella isolated in humans and animals in latin america from 1968 to 2006. | we report a retrospective analysis of 1933 brucella strains isolated from humans and animals in latin american countries between 1968 and 1991 and in argentina between 1994 and 2006. during the first period 50% of strains were from humans, mainly from argentina, mexico and peru but, while b. suis was the main cause of infection in argentina, b. melitensis was responsible for most infections in the other countries. in argentina in the later years, b. melitensis and b. suis were observed more freq ... | 2008 | 17559694 |
| brucella isolated in humans and animals in latin america from 1968 to 2006. | we report a retrospective analysis of 1933 brucella strains isolated from humans and animals in latin american countries between 1968 and 1991 and in argentina between 1994 and 2006. during the first period 50% of strains were from humans, mainly from argentina, mexico and peru but, while b. suis was the main cause of infection in argentina, b. melitensis was responsible for most infections in the other countries. in argentina in the later years, b. melitensis and b. suis were observed more freq ... | 2008 | 17559694 |
| demonstration of is711 transposition in brucella ovis and brucella pinnipedialis. | the brucella genome contains an insertion sequence (is) element called is711 or is6501, which is specific to the genus. the copy number of is711 varies in the genome of the different brucella species, ranging from 7 in b. abortus, b. melitensis and b. suis to more than 30 in b. ovis and in brucella strains isolated from marine mammals. at present, there is no experimental evidence of transposition of is711, but the occurrence of this element with a high copy number in some species, and the isola ... | 2008 | 18218072 |
| brucella microti sp. nov., isolated from the common vole microtus arvalis. | two gram-negative, non-motile, non-spore-forming, coccoid bacteria (strains ccm 4915(t) and ccm 4916), isolated from clinical specimens of the common vole microtus arvalis during an epizootic in the czech republic in 2001, were subjected to a polyphasic taxonomic study. on the basis of 16s rrna (rrs) and reca gene sequence similarities, both isolates were allocated to the genus brucella. affiliation to brucella was confirmed by dna-dna hybridization studies. both strains reacted equally with bru ... | 2008 | 18218934 |
| putative quorum-sensing regulator blxr of brucella melitensis regulates virulence factors including the type iv secretion system and flagella. | brucella melitensis is an intracellular pathogen that establishes a replicative niche within macrophages. while the intracellular lifestyle of brucella is poorly understood and few virulence factors have been identified, components of a quorum-sensing pathway in brucella have recently been identified. the luxr-type regulatory protein, vjbr, and an n-acylhomoserine lactone signaling molecule are both involved in regulating expression of the virb-encoded type iv secretion system. we have identifie ... | 2008 | 18310341 |
| brucella melitensis b115-based complement fixation test to detect antibodies induced by brucella rough strains. | to assess the efficiency of a brucella melitensis b115 rough strain, naturally devoid of anticomplementary activity, used as antigen in a complement fixation test (cft) to detect antibodies induced by brucella strains with rough phenotype, such as brucella abortus rb51, brucella ovis and brucella canis. | 2008 | 18355234 |
| rapid polymerase chain reaction-based screening assay for bacterial biothreat agents. | to design and evaluate a rapid polymerase chain reaction (pcr)-based assay for detecting eubacteria and performing early screening for selected class a biothreat bacterial pathogens. | 2008 | 18370996 |
| brucella melitensis, b. neotomae and b. ovis elicit common and distinctive macrophage defense transcriptional responses. | brucella spp. establish an intracellular replicative niche in macrophages, while macrophages attempt to eliminate the bacteria by innate defense mechanisms. brucella spp. possess similar genomes yet exhibit different macrophage infections. few b. melitensis and b. neotomae enter macrophages with intracellular adaptation occurring over 4-8 hr. conversely, b. ovis are readily ingested by macrophages and exhibit a persistent plateau of infection. evaluating early macrophage interaction with brucell ... | 2009 | 19934366 |
| a novel brucella isolate in association with two cases of stillbirth in non-human primates - first report. | brucellosis is veterinary and human health problem. | 2009 | 19187435 |
| brucella tir domain-containing protein mimics properties of the toll-like receptor adaptor protein tirap. | toll-like receptors (tlrs) play essential roles in the activation of innate immune responses against microbial infections. tlrs and downstream adaptor molecules contain a conserved cytoplasmic tir domain. tirap is a tir domain-containing adaptor protein that recruits the signaling adaptor myd88 to a subset of tlrs. many pathogenic microorganisms subvert tlr signaling pathways to suppress host immune responses to benefit their survival and persistence. brucella encodes a tir domain-containing pro ... | 2009 | 19196716 |
| real-time pcr for identification of brucella spp.: a comparative study of is711, bcsp31 and per target genes. | culture is considered as the reference standard assay for diagnosis of brucella spp. in humans and animals but it is time-consuming and hazardous. in this study, we evaluated the performances of newly designed real-time pcr assays using taqman probes and targeting the 3 following specific genes: (i) the insertion sequence is711, (ii) bcsp31 and (iii) per genes for the detection of brucella at genus level. the real-time pcr assays were compared to previously described conventional pcr assays targ ... | 2009 | 19200666 |
| intracellular adaptation of brucella abortus. | macrophages were infected with virulent brucella abortus strain 2308 or attenuated strain 19. intracellular bacteria were recovered at different times after infection and their proteomes compared. the virulent strain initially reduced most biosynthesis and altered its respiration; adaptations reversed later in infection. the attenuated strain was unable to match the magnitude of the virulent strain's adjustments. the results provide insight into mechanisms utilized by brucella to establish intra ... | 2009 | 19216536 |
| rapid differential diagnosis between extrapulmonary tuberculosis and focal complications of brucellosis using a multiplex real-time pcr assay. | arduous to differ clinically, extrapulmonary tuberculosis and focal complications of brucellosis remain important causes of morbidity and mortality in many countries. we developed and applied a multiplex real-time pcr assay (m rt-pcr) for the simultaneous detection of mycobacterium tuberculosis complex and brucella spp. | 2009 | 19225565 |
| analysis of ten brucella genomes reveals evidence for horizontal gene transfer despite a preferred intracellular lifestyle. | the facultative intracellular bacterial pathogen brucella infects a wide range of warm-blooded land and marine vertebrates and causes brucellosis. currently, there are nine recognized brucella species based on host preferences and phenotypic differences. the availability of 10 different genomes consisting of two chromosomes and representing six of the species allowed for a detailed comparison among themselves and relatives in the order rhizobiales. phylogenomic analysis of ortholog families show ... | 2009 | 19346311 |
| lab on a chip genotyping for brucella spp. based on 15-loci multi locus vntr analysis. | brucellosis is an important zoonosis caused by the genus brucella. in addition brucella represents potential biological warfare agents due to the high contagious rates for humans and animals. therefore, the strain typing epidemiological tool may be crucial for tracing back source of infection in outbreaks and discriminating naturally occurring outbreaks versus bioterroristic event. a multiple locus variable-number tandem repeats (vntr) analysis (mlva) assay based on 15 polymorphic markers was pr ... | 2009 | 19351390 |
| comparative proteomics analyses reveal the virb of b. melitensis affects expression of intracellular survival related proteins. | brucella melitensis is a facultative, intracellular, pathogenic bacterium that replicates within macrophages. the type iv secretion system encoded by the virb operon (virb) is involved in brucella intracellular survival. however, the underlying molecular mechanisms, especially the target proteins affected by the virb, remain largely unclear. | 2009 | 19401764 |
| characterization of possible correlates of protective response against brucella ovis infection in rams immunized with the b. melitensis rev 1 vaccine. | vaccination with the live attenuated brucella melitensis rev 1 vaccine is used to control ovine brucellosis caused by brucella ovis in sheep. the objective of this study was to identify possible correlates of protective response to b. ovis infection through the characterization by microarray hybridization and real-time rt-pcr of inflammatory and immune response genes differentially expressed in rams previously immunized with b. melitensis rev 1 and experimentally challenged with b. ovis. gene ex ... | 2009 | 19428917 |
| pathogenicity of different strains of histophilus somni in the experimental induction of ovine epididymitis. | the purpose of this study was to determine any differences in pathogenicity when sheep are experimentally infected with different histophilus somni isolates: a) 2336 bovine origin strain; b) an isolate from ram orchitis and epididymitis; c) an isolate from the brain of a sheep with neurological signs; d) an isolate from the vagina of a clinically healthy ewe. a total of 20 rams divided in groups of 5 animals each were inoculated in the epididymis with 1 x 10(7) cfu/ml of h. somni; a negative con ... | 2009 | 19436586 |
| genome degradation in brucella ovis corresponds with narrowing of its host range and tissue tropism. | brucella ovis is a veterinary pathogen associated with epididymitis in sheep. despite its genetic similarity to the zoonotic pathogens b. abortus, b. melitensis and b. suis, b. ovis does not cause zoonotic disease. genomic analysis of the type strain atcc25840 revealed a high percentage of pseudogenes and increased numbers of transposable elements compared to the zoonotic brucella species, suggesting that genome degradation has occurred concomitant with narrowing of the host range of b. ovis. th ... | 2009 | 19436743 |
| design and influence of gamma-irradiation on the biopharmaceutical properties of nanoparticles containing an antigenic complex from brucella ovis. | despite vaccination campaigns, brucellosis is still one of the most common bacterial zoonosis in the world. this work describes the development of a novel formulation strategy to the delivery of the brucella ovis antigenic extract (hs) into ovine mucosal surfaces. thus, hs was entrapped in conventional and mannosylated poly(anhydride) nanoparticles by the solvent displacement method, and the resulting nanosystems were gamma-irradiated to accomplish the sterilization required for the ophthalmic a ... | 2009 | 19442721 |
| first evidence of brucella ovis infection in republic of croatia. | we researched the spread of brucella ovis (b. ovis) infection in sheep during 2002 and 2003 in croatia. a total of 30,635 sheep blood samples were examined using the enzyme-linked immunosorbent assay (elisa). in 2002, 1014 out of 14,404 examined sheep blood samples (7%) from six counties gave positive reactions while 2060 (14.3%) were found suspicious. in 2003, 638 out of 16,221 examined sheep blood samples in nine counties (3.9%) tested positive while 1083 (6.7%) were suspicious. in rams and sh ... | 2009 | 19537042 |
| a novel nanoparticulate adjuvant for immunotherapy with lolium perenne. | specific immunotherapy implies certain drawbacks which could be minimized by the use of appropriate adjuvants, capable of amplifying the right immune response with minimal side effects. in this context, previous studies of our group have demonstrated the adjuvant capacity of gantrez an nanoparticles, which can effectively enhance the immune response. in this work, two types of nanoparticles (with and without lps of brucella ovis as immunomodulator) with encapsulated lolium perenne extract are te ... | 2009 | 19545572 |
| rapid real-time pcr assays for detection of klebsiella pneumoniae with the rmpa or maga genes associated with the hypermucoviscosity phenotype: screening of nonhuman primates. | the relationship of mucoviscosity-associated (maga) and/or regulator of mucoid phenotype (rmpa) genes to the klebsiella pneumoniae hypermucoviscosity (hmv) phenotype has been reported. we previously demonstrated that rmpa+ k. pneumoniae can cause serious disease in african green monkeys and isolated rmpa+ and maga+ hmv k. pneumoniae from other species of non-human primates. to rapidly screen african green monkeys/non-human primates for these infections, we developed three real-time pcr assays. t ... | 2009 | 19644019 |
| brucella microti: the genome sequence of an emerging pathogen. | using a combination of pyrosequencing and conventional sanger sequencing, the complete genome sequence of the recently described novel brucella species, brucella microti, was determined. b. microti is a member of the genus brucella within the alphaproteobacteria, which consists of medically important highly pathogenic facultative intracellular bacteria. in contrast to all other brucella species, b. microti is a fast growing and biochemically very active microorganism with a phenotype more simila ... | 2009 | 19653890 |
| efflux-mediated drug resistance in bacteria: an update. | drug efflux pumps play a key role in drug resistance and also serve other functions in bacteria. there has been a growing list of multidrug and drug-specific efflux pumps characterized from bacteria of human, animal, plant and environmental origins. these pumps are mostly encoded on the chromosome, although they can also be plasmid-encoded. a previous article in this journal provided a comprehensive review regarding efflux-mediated drug resistance in bacteria. in the past 5 years, significant pr ... | 2009 | 19678712 |