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[experimental studies on the pathogenesis of coli-enterotoxemia in swine. 4. effect of lipopolysaccharide endotoxin on weaned piglets following parenteral administration].ten clinically intact weaned piglets were experimentally intoxicated by intravenous injection of lipoproteide-free lipopolysaccharide endotoxin according to westphal of e. coli o 127:b8. severe endotoxin shock with all clinical manifestations of experimental coli-enterotoxaemia was induced in all animals and included circulatory disorder with tachycardia, intermittent pallor and/or cyanosis, symptoms of severe systemic intoxication, neurological symptoms, such as lack of coordination, hindleg st ...1977332109
[enterotoxin-producing e.-coli bacteria in enteritis and edema disease of swine. quick diagnosis of heat-labile and heat resistant toxin]. 1977332152
effect of heat stable and heat labile escherichia coli enterotoxins, cholera toxin and theophylline on unidirectional sodium and chloride fluxes in the proximal and distal jejunum of weanling swine.acute, isolated loops of proximal and distal jejunum of weanling swine were exposed to either heat stable porcine escherichia coli enterotoxin, heat labile porcine escherichia coli enterotoxin, cholera toxin or theophylline. unidirectional sodium fluxes in response to heat stable in the proximal jejunum were dependent on the length of time that the intestinal mucosae was exposed to the enterotoxin. net water, sodium and chloride and unidirectional sodium and chloride flux measurements in the pro ...1977332292
breeding record analysis in pig herds and its veterinary applications--2: experiences with a large commercial unit.a computer program was used to analyse data from a large commercial pig unit over two years to assess reproductive efficiency and the efficiency of weaner production. monthly inspections of the farm and quarterly post mortem examinations of all dead pigs provided further information. the effects of controlled changes in management on production were assessed. overall the number of piglets reared per sow per year was increased from 15-1 to 17-2 and the eight-week weights improved from a mean of 1 ...1977335631
human milk and gut infection in the newborn. 1977336123
uptake of maternal antibody by the neonatal pig following intramuscular and intramammary vaccination of the preparturient sow.administration of heat inactivated escherichia coli antigens by intramuscular and intramammary routes induced elevated antibody levels in sow serum and colostrum, predominantly associated with igg. colostral igg accounted for approximately 80 per cent of the total antibody activity, and there was a similar distribution in the sera of one-day-old piglets. the additional antibody activity was carried almost entirely by igm following intramuscular injections and was evenly distributed between igm a ...1977337428
effects of various nutrient levels and enviromental temperatures on the incidence of colibacillary diarrhea in pigs: intestinal fistulation and titration studies. 1977340437
alternatives to aspirin, derived from biological sources. 1977343554
detecting enteropathogenic escherichia coli strains of porcine origin. 1. correlations between o and k antigens and the enterotoxin production in strains isolated from the newborn piglet.a study of enterotoxin production by means of biological tests (y1 adrenal cells and suckling mice) using 67 escherichia coli strains responsible for diarrhea in newborn piglets, confirmed the advantages of these methods and demonstrated the correlation between antigenic specificity and enterotoxin production. in particular the presence of capsular antigen k 88 is related to the thermolabile (lt) fraction of the enterotoxin in 73.9 % of the enteropathogenic strains studied.1977343700
preweaning mortality in the pig. pathological findings in piglets dying between birth and weaning.during a survey of preweaning mortality 538 piglets which died between birth and weaning were autopsied. the results of laboratory examinations permitted a division of the findings into a number of syndromes which were considered to be associated with the immediate cause of death. the non-infectious conditions such as trauma, starvation and suffocation were the most common. seventy-eight piglets were included in the trauma group because of depressed cranial bone fractures or visceral rupture lea ...1977200212
survival times of bacteria in liquid manure. 1977382807
septic shock in obstetrics and gynecology. pathophysiology. 1977407404
acrolein. 1978415230
[causative agents of category-ii escherichiasis and the results of studies testifying to their circulation in the ussr]. 1978394394
electron paramagnetic resonance spectra of myeloperoxidase in polymorphonuclear leukocytes. 1978202508
mechanisms in the pathogenesis of diarrhea: a review. 1978203563
study of the in vitro pharmacological effects of gram-negative endotoxins in parallel with toxicity testing in normal adult guinea-pigs. 1978206397
the effect of cholera toxin and heat labile and heat stable escherichia coli enterotoxin on cyclic amp concentrations in small intestinal mucosa of pig and rabbit.the effect of cholera toxin, heat labile and heat stable escherichia coli enterotoxin on mucosal cyclic amp concentrations was determined on the proximal jejunum of weanling pigs and young rabbits. ligated loops were injected with solutions containing no enterotoxin for control and either cholera toxin, heat labile or heat stable e. coli enterotoxin. the loops were drained after either two, four or six hours incubation at which time accumulated fluid was recorded and mucosal samples removed for ...1978210913
panel report on the colloquium on selected diarrheal diseases of the young. 1978212391
naturally occurring and experimentally induced rotaviral infections of domestic and laboratory animals. 1978212392
elisa: enzyme-linked immunosorbent assay.similar in design to radioimmunoassay, comparable in sensitivity and specificity but easier, safer, and less expensive, this new diagnostic technique uses enzyme-labeled rather than isotope-labeled reagents. the end point is a color change that can be assessed by colorimetry or with the naked eye. various techniques of elisa are described, along with examples of current and potential clinical applications.1978215505
purification and properties of a pig heart thiolase with broad chain length specificity and comparison of thiolases from pig heart and escherichia coli.a thiolase (acetyl coa acyltransferase, ec 2.3-1.16) which acts on substrates of various chain lengths (thiolase i) has been purified from pig heart muscle 366-fold to near homogeneity as judged by gel electrophoresis. its molecular weight was estimated to be 200,000 in the absence and 46,000 in the presence of sodium dodecyl sulfate. kinetic measurements with acetoacetyl coenzyme a, 3-ketohexanoyl-coa, 3-ketooctanoyl-coa, and 3-ketodecanoyl-coa yielded apparent km values of 16, 8.3, 2.4, and 1. ...1978344310
microbial iron-chelators and their action on klebsiella infections in the skin of guinea-pigs.preparations of catechols from ethyl acetate extracts of cultures of klebsiellae in a low-iron medium contained iron-chelators whose potency was measured by the reversal of the bacteristasis of escherichia coli and klebsiellae in unheated horse serum, and of the growth-inhibition of these two organisms by ethylene diamine di-orthohydroxyphenyl acetic acid (edda). as revealed by in situ tests of paper chromatograms, there was a multiplicity of biologically active chelators in the preparations. ca ...1978350255
[animal tissue polynucleotide phosphorylase].localization, physico-chemical and catalytic properties and possible biological functions of polynucleotide phosphorylase (pnpase) from animal tissues are discussed. in animal tissue cells pnpase has multiple localization; the major amount of the enzyme is localized in the endoplasmic reticulum ribosomes. in the nuclei pnpase, similar to other endo- and exo-rnases participates in the processing of precursor molecules of mature forms of rna, whereas in the cytoplasm it is involved in the destruct ...1978350293
hydrogen sulphide-positive strains of escherichia coli from swine.thirty-two h2s-positive strains of escherichia coli, isolated from the caecal contents or mesenteric lymph nodes or both of 60 apparently healthy pigs, were characterized. eighteen different serotypes and 18 different fermentative types were found. during conjugation with e. coli k12, four strains transferred their hys plasmids linked to raf, and another transferred hys associated with the tc determinant. all hys-raf plasmids belong to incompatibility group fi, whilst tc-hys is an fi- plasmid.1978351185
characterization of plasmids that encode for the k88 colonization antigen.k88 antigen, and important virulence factor in porcine enteropathogenic escherichia coli (eec), can be transferred along with the ability to ferment the trisaccharide raffinose (raf). the plasmids from a number of eec strains that encode these two properties were isolated and characterized. in most strains the k88 and raf genes were found on a single nonconjugative plasmid approximately 50 x 10(6) daltons in size. this plasmid core was conserved with only slight variation among the strains teste ...1978352948
effect of heat stable and heat labile escherichia coli enterotoxins and cholera toxin in combination with theophylline on unidirectional sodium and chloride flux in the small intestine of weanling swine.the effect of heat stable and heat labile escherichia coli enterotoxins or cholera toxin in combination with theophylline on net water, sodium and chloride and unidirectional sodium and chloride fluxes was examined in acute isolated loops of jejunum of weanling swine. the effect of heat stable enterotoxin in combination with theophylline was determined in loops located in the proximal jejunum, while combinations of theophylline and either heat labile enterotoxin or cholera toxin were studied in ...1978356942
in vitro adhesion of escherichia coli to porcine small intestinal epithelial cells: pili as adhesive factors.escherichia coli strains with pili (k99 or 987p) known to facilitate intestinal colonization adhered in vitro to porcine intestinal epithelial cells. these strains adhered equally to both ileal and jejunal epithelial cells. a laboratory e. coli strain that has type 1 pili also adhered to porcine intestinal epithelial cells. when nonpiliated cells derived from 987p+, k99+, or type 1 pilus+ strains were used for in vitro adhesion assays, they failed to adhere. the attachment of piliated bacteria t ...1978357285
biological evaluation of a methanol-soluble, heat-stable escherichia coli enterotoxin in infant mice, pigs, rabbits, and calves.escherichia coli p16 was shown to produce two heat-stable toxins (st) with differing biological activity. the toxins were separated by methanol extraction, and the first, sta, was methanol soluble, partially heat stable, active in neonatal piglets (1 to 3 days old) and infant mice, but inactive in weaned pigs (7 to 9 weeks old); the second, stb, was methanol insoluble, active in weaned pigs and rabbit ligated loops, but inactive in infant mice. it is therefore suggested that use of suckling mice ...1978357288
[various aspects of competitive relationship between shigella and escherichia coli].one of the aspects of antagonistic relations could be the competence of microorganisms for specific sites of attachement to the epithelium common for shigellae and some e. coli serological types; this was demonstrated on a model of shigella-induced keratoconjunctivitis and in experiments with the hep-2 cells infected with h3-glucose-labeled sh. flexneri 5a, with combined administration of the latter with e. coli 08 and 028. the data obtained emphasized the importance for the development of the i ...1978358685
[natural antibodies (isohemagglutinins, heterophilic, antierythrocytic and antibacterial) in children with congenital immunologic deficiency syndromes].examination of 30 patients with immunodeficient diseases showed that the system of natural antibodies was considerably changed depending on the form and the extent of deficiency of the immune system: in agammaglobuinemia the antibodies under study were almost completely absent, and in immunological insufficiency with ataxia-teleangiectasis the production of antibacterial antibodies proved to be sharply decreased. the data obtained can be used both for the diagnosis of immunodeficiencies and for ...1978358690
proteinase, lipase and amylase activities in the small intestine of pigs suffering from colienterotoxaemia. 1978358811
superiority of human complement for assaying bacterial lipopolysaccharides by their anticomplementary activity.in assaying bacterial lipopolysaccharides (lps) for anticomplementary activity, human complement (c) allowed detection of approximately 200 times smaller amounts of lps than guinea-pigs c. pig c was slightly inferior to human.1978363447
the influence of plasmid-determined and other characteristics of enteropathogenic escherichia coli on their ability to proliferate in the alimentary tracts of piglets, calves and lambs. 1978364065
[production of live attenuated vaccine against colibacillosis in pigs].two strains of beta-hemolytic escherichia coli, p-0141 and i-0149 were used to obtain mutant e. coli organisms with unchanged cultural, morphological, biochemical, and serological properties. the mutants proved apathogenic for albino mice at intravenous injection of a 6-hour broth culture at the rate of 0.3 cm3 or an endotoxin in the same dose. neither were they enteropathogenic for pigs when inoculated into intestinal segments. they could be used as vaccinal strains.1978369110
studies on escherichia coli in pigs v. determination of enterotoxicity and frequency of o groups and k 88 antigen in strains from 200 piglets with neonatal diarrhoea. 1978369239
detection of iga anti-escherichia coli plasma cells in the intestine and salivary glands of pigs orally and locally infected with e. coli. 1978369316
[cerebrospinal angiopathy after experimental protracted shock (author's transl)]. 1978371211
the sources of "oh" serotypes of escherichia coli.the sources from which different "oh" serotypes of escherichia coli have been isolated are listed in a series of tables. it is considered that these tables should provide a basis for obtaining a greater understanding of the ecology of e. coli.1978340583
immunological aspects of cell membrane adhesion demonstrated by porcine enteropathogenic escherichia coli. 197875179
escherichia coli strains that cause diarrhoea but do not produce heat-labile or heat-stable enterotoxins and are non-invasive.three enteropathogenic escherichia coli (e.p.e.c.) strains (o127:k63:h6, o128:k67:h2, and o142:k86:h6) isolated from outbreaks of infantile diarrhoea and one strain from the "normal" colonic flora (e. coli hs) of a healthy adult were fed in doses of 10(6), 10(8), and 10(10) organisms in nahco3 to adult volunteers. the strains, which had been stored for 7--9 years, gave negative results in sensitive tests for heat-labile (l.t.) enterotoxin (y-1 adrenal-cell test), heat-stable (s.t.) enterotoxin ( ...197877415
detecting enteropathogenic strains of escherichia coli of porcine origin. 2. relationship between o and k antigens and the production of enterotoxin in strains isolated from the piglet after weaning.the production of enterotoxin by biological tests (yl adrenal cells and the suckling mouse) has been examined in 96 strains of escherichia coli, isolated from sick piglets after weaning. there is a good correlation between the presence of the capsular antigen k88 and that of the thermolabile fraction (lt) of the enterotoxin (69.2 per cent of the enteropathogenic strains studied). however, the presence of the thermostable fraction (st) of the enterotoxin of strains which, according to their serol ...197886320
gnotobiotic pigs-derivation and rearing.the procurement, rearing, nutrition and microbiological monitoring of gnotobiotic pigs and a method for conditioning of primary, colostrum-deprived, specific pathogen free pigs is described. as compared to the established hysterectomy and closed hysterotomy methods for the derivation of gnotobiotic piglets an alternative approach, open caesarian section with the sow maintained under general halothane-nitrous oxide anaesthesia and the introduction of each fetus into the sterile isolator via a liq ...1978154359
incidence and relationship of group d streptococci with other indicator organisms in meats.raw and processed meats were analyzed for presumptive group d streptococci using kf streptococcus agar. counts were compared with coliform, presumptive escherichia coli, and enterobacteriaceae counts but no meaningful relationships were observed. results indicated that group d streptococci and e. coli type i were principally contaminants from the packing plant, rather than at retail level. the predominating group d streptococcus in both beef and pork cuts was streptococcus faecalis, while in pro ...1978106944
[demonstration of enterotoxin-forming pseudomonas aeruginosa strains in the intestinal ligature test in piglets]. 1979119390
[preliminary results of an atiepizootic field test using edta-na extract vaccine from escherichia coli strains pathogenic to swine in swine production industrial units].an anti-epizootic field test was applied to industrialised pig farms in the region of wrocław, poland, to test the effectiveness of a pentavalent edta (calcium disodium edetate)--sodium vaccine extracted from escherichia coli strains with pathogenicity to swine. the vaccine had been received from a centre in berlin-buch, gdr. the vaccine failed to provide any protection, when orally applied to nursed piglets. however, both morbidity and mortality were reduced and, thus, an anti-epizootic effect ...1979121226
[observations on antibacterial activity of thiamphenicol glycinate acetylcysteinate (author's transl)].in vitro thiamphenicol antibacterial activity in the presence of n-acetylcysteine as sodium salt proved to be enhanced when compared with that of the antibacterial alone against both gram-positive and gram-negative bacterial strains. the in vitro results were confirmed in vivo in the guinea-pig, where the sera from animals previously treated by i.m. route with thiamphenicol glycinate acetylcysteinate, showed a higher antibacterial activity than sera from animals treated with thiamphenicol glycin ...1979122158
diastereoisomeric glucuronides of oxazepam. isolation and stereoselective enzymic hydrolysis.oxazepam glucuronide isolated from swine urine by previously published methods was separated into its diastereoisomers by ion-exchange chromatography on a preparative scale. quantitative high-performance liquid chromatography was used to monitor the separation. the two isomers were obtained in analytically pure form and then characterized by elemental analysis, oxazepam content, mass spectrometry, ultraviolet spectroscopy, optical rotation and optical rotatory dispersion-circular dichroism. the ...197935325
an examination of the o and k specificity involved in the antibody-induced loss of the k88 plasmid from porcine enteropathogenic strains of escherichia coli.the heat-labile k88 antigen, a virulence determinant coded for by a transmissible plasmid, was eliminated from enteropathogenic strains of escherichia coli by passage through media containing antibodies to the heat stable antigens of an abbotstown (o149:k91,k88ac) strain. the plasmid-curing activity of o149 antisera was not o-antigen specific as o149, o45, o8 and o138 strains of e. coli could be 'cured' of their k88 plasmids by this technique. the curing activity was differentiated from the o-an ...197992455
drug resistance in escherichia coli isolated from diarrhoeic piglets. 197992850
relationships among raffinose plasmids determined by the immunochemical cross-reaction of their alpha-galactosidases.plasmid-encoded alpha-galactosidase served as a marker enzyme for the recognition and comparison of raffinose (raf) plasmids present in strains of escherichia coli. immunochemical relationships were established among raf plasmids of 39 independent isolates from man and domestic animals (from three continents) by using antiserum against alpha-galactosidase. immunodiffusion revealed three serological subclasses of alpha-galactosidase, which are correlated with the biological and geographical origi ...197994343
mechanisms of immunity in bacterial infections. 197995156
[evaluation of the efficacy of carbadox as a growth stimulant by microbiological criteria]. 1979375628
influence of o and k antigens on the surface properties of escherichia coli in relation to phagocytosis.strains of escherichia coli with different o and k antigens were investigated with respect to physicochemical surface characteristics and liability to phagocytosis. using two-phase partitioning analysis for the surface characterization, three main groups of strains emerged: group i (o1:k1, o2:k1, o3:k2ab) showing both smooth hydrophilic o antigens and negatively-charged k antigen which rendered the strains maximally resistant to phagocytosis. group ii (o55:k59, o111:k58) showed no acidic k antig ...1979375674
effect of circulating, maternally derived antibody on the development of a local immune response in the intestine of the neonatal pig. 1979378047
renal damage due to urinary tract infection.chronic pyelonephritis is a disease of childhood which is carried on into adult life. children under the age of 4 in whom uti, vesico-ureteric reflux and pyelotubular back-flow coexist appear to be particularly at risk of developing kidney damage. attempts to prevent kidney damage in adults by detection and treatment of covert uti are doomed to failure because persistent bacteriuria in the adult is a consequence of underlying abnormality of the urinary tract rather than its cause. our main hope ...1979380901
immunological studies in mice with swine edema disease principle (neurotoxin). 1979382704
contamination of the deep tissues of carcasses by bacteria present on the slaughter instruments or in the gut. 1979222722
evidence for bactericidal activity of polymorphonuclear leukocytes without phagocytosis.the relationship between phagocytosis and bactericidal action of polymorphonuclear leukocytes was examined by comparing the functions of cytochalasin d-treated leukocytes with those of the control. measurement of phagocytotic and bacterial dna-degrading activities using escherichia coli prelabeled with [3h]thymidine revealed that phagocytosis and bacterial dna degradation were inhibited by treatment with cytochalasin d to about 50 and 10% of the control, respectively. nevertheless, the bacterici ...1979225308
the aetiology of diarrhoea in pigs weaned at two days of age.when pigs are weaned at two days of age large numbers of excherichia coli appear in the anterior gut and the incidence of diarrhoea rises. the two phenomena do not appear to be directly related because the strains of e coli isolated are not serotypes previously found to be associated with neonatal pig scouring. representative strains of the non-enteropathogenic serotypes did not produce enterotoxin and did not adhere to small intestine brush borders. moreover when antibiotics were fed to elimina ...1979228367
the role of thromboxane a2 in endotoxin-induced aggregation of guinea-pig platelets in vitro.large concentrations of endotoxin lipopolysaccharides (lps) e. coli o127:b8 and e. coli o26:b6 were needed for induction of platelet aggregation in citrated platelet rich plasma (prp) from normal guinea-pigs. when guinea-pigs were actively immunized with lps e. coli o127:b8 their prp became more sensitive to the aggregatory effects of this type of lps. the increase in sensitivity was rather selective since the responses to adp and lps e. coli o26:b6 remained unchanged. in endotoxin-stimulated pr ...1979386740
aspartate transaminase from e. coli: amino acid sequences of the nh2-terminal 33 residues and chymotryptic pyridoxyl tetrapeptide. 1979387032
relationship between the intestinal permeability to macromolecules and invasion of septicemia-inducing escherichia coli in neonatal piglets.the influence of age and diet on the invasion of septicemia-inducing escherichia coli and the endocytotic activity of the small intestinal epithelium were examined in colostrum-deprived conventional and gnotobiotic piglets orally infected with e. coli 078. the piglets infected at birth and the animals fed glucose-amino acids solution and infected at 3 days after birth soon suffered from septicemia caused by the invasion of e. coli 378. the piglets fed artifical milk and infected at 3 days after ...1979387609
determinants of the action of phospholipases a on the envelope phospholipids of escherichia coli. 1979387763
[effect of reduced gastrointestinal motility on the regulation of gastrointestinal flora and the pathogenesis of coli enterotoxinemia in market swine].opium tincture and spasmentral were applied to piglets early after weaning and reduced their gastro-intestinal motility, which, however, caused only very minor changes in quantitative germ flora composition in those first days. short-time suppression of gastro-intestinal motility obviously does not result in detrimental consequences to the organism as a whole, since there seem to be several factors which are involved in the control and regulation of the intestinal germ flora. impairment of gastr ...1979389190
the ability of cholestyramine resin and other adsorbents to bind escherichia coli enterotoxins.several adsorbent materials were evaluated for their ability to bind escherichia coli enterotoxins. cholestyramine, a strong anion-exchange resin, bound the heat-labile and the heat-stable types of enterotoxin and reduced significantly their effects in some animal models. however, its efficacy in the treatment of diarrhoeic piglets appeared to be adversely affected by the presence of milk in the alimentary tract.1979390155
the binding of lps to the lymphocyte surface.bacterial lipopolysaccharide (lps) from escherichia coli labelled with tritium has been used to follow the binding of lps to lymphocytes. binding to cells rose to a maximum 2-7 min after addition of [3h]-lps, followed by loss of [3h]-lps from cells, reducing to about 10% of the peak level at 20-30 min. peripheral blood lymphocytes, mesenteric lymph node and thymus cells of the pig and cba, c3h/he and c3h/hej mouse spleen cells all bound [3h]-lps transiently at similar levels. it is concluded tha ...1979391698
changes in the intestinal flora of young pigs with postweaning diarrhea or edema disease. 1979393690
duration of anti-adhesive and bactericidal activities of milk from vaccinated sows on escherichia coli o149 in the digestive tract of piglets during the nursing period.piglets were exposed orogastrically to escherichia coli to enable study of the duration of anti-adhesive and bactericidal activities of milk of sows vaccinated with a k88 enriched e coli vaccine. there was a marked increase in the number of the challenge strain in the digestive tract of weaned piglets of all ages (between 888 and 2144 per cent). in contrast, there was a decrease in their number (75 per cent) in the day-old colostrum-fed piglets. when the piglets were two weeks old milk was still ...1979396618
[immunobiological activity of various blood serum fractions from cattle and swine. the dynamics of ig in specific serum against colibacillosis in pigs].the level of the igm and igg agglutinating antibody specific hyperimmune serum against coli-bacteriosis and its fractions were studied. the serum was obtained at various stages of the preparation, hyperimmunization and exploitation of serum producing swine by using the 2-mercapto-ethanol test. it was established that serum of the total globulin, alpha-beta-globulin and gamma-globulin fractions contains igm and igg agglutinating antibodies against e. coli. these immunoglobulin classes are synthet ...1979396718
some biological characteristics of escherichia coli strains isolated from acute diarrhoeal diseases of infants.the aim of this study was to evaluate the methods of biological characterization of escherichia coli strains, in order to use them as screening tests in clinical microbiological laboratories. in two thirds of the 45 e. coli, isolated from acute intestinal infection of infants, different factors of virulence were found. the strains showed prevalently an enterotoxigenic character (66,6%), which was in correlation with the increased permeability (62,2%). adhesive properties were present in 20% of e ...1979396738
course of antibiotic sensitivities in escherichia coli and staphylococcus aureus from animals.in order to establish the evolution of resistance against the common antibiotics from the beginning of their use up to the present time, the sensitivity of escherichia coli isolated from the cecum of wild boars was determined. the mic's of e. coli from these animals in a zoological garden was examined for comparison. the samples from the wild boars were collected in five forests in the belgian ardennes. the mic's obtained (table 1) are comparable to the mean values given in the early literature ...1979397700
enterotoxins of enteric bacteria--a review.a review of the enterotoxins of enteric bacteria was compiled, taking into account recent developments of the field as well as results of the author's own research work. after characterization of enterotoxins of different origin, their pathogenic role is described, with special attention to the genetic background of these agents. the possible use of enterotoxins for active immunization purposes is also discussed.1979399581
[activity of various enzymes in the blood, liver, intestines and kidneys in spontaneous colibacteriosis of piglets].the purpose of the studies was to determine the activity of enzymes in the serum and pig organs with colibacteriosis in the form of oedema and stomach-intestines disease playing a diagnostic role in the determination of the organ damage degree. studies were carried out on 29 pigs. the activity of lap, gpt, got, am, fdpa, lp, ap and acp was determined in the serum liver, empty intestines and kidneys of the diseased and control pigs. it resulted from the experiments that in the serum of all the di ...1979400185
factors contributing to postweaning diarrhoea in a large intensive piggery.some aspects of postweaning diarrhoea (pwd) in a piggery during the first week after early weaning were investigated. a haemolytic enterotoxigenic strain of e. coli (o149:k88:h10) was regularly recovered from piglets with pwd while rotavirus was demonstrated on a number of occasions. prior to weaning piglets were either free of, or shed very few, haemolytic e. coli in their faeces. however, all piglets were excreting haemolytic e. coli between 5 and 7 days after weaning. the role of rotavirus in ...19806252883
[nuclear translocation and effect of camp-dependent protein kinase on transcription].the influence of camp-dependent pig brain protein kinase and its subunits on transcription in vitro was studied. the increase in the template activity of chromatin isolated from the nuclei after treatment with the catalytic subunit was observed. the regulatory subunit of protein kinase was found to increase the number of binding sites for rna polymerase on chromatin. the camp-dependent pig brain protein kinase was found to phosphorylate the sigma-factor of escherichia coli rna polymerase. this p ...19806260240
wound infection: a controlled clinical and experimental demonstration of synergy between aerobic (escherichia coli) and anaerobic (bacteroides fragilis) bacteria.pathogenic synergy between escherichia coli and bacteroides fragilis was suggested by clinical trials and proved to exist in an experimental guinea-pig wound model. this finding is thought to have important theoretical and practical therapeutic implications.19806987938
antibacterial antibodies in swine colostrum.antibacterial antibody activities were examined in 138 samples of swine colostrum by o-agglutinin test with serotypes of escherichia coli (o-6, 0-45, 0-81, 0-83, 0-115, 0-138, o-139, and o-126), salmonella typhimurium, and salmonella cholerae suis. the mean agglutinin titer of e coli o-83 was the highest; e coli o-115 was next highest. the mean agglutinin titer of s cholerae suis was lowest; s typhimurium was next to lowest. correlation coefficients among these o-agglutinin titers were between 0 ...19806989301
influence of oral antibiotics on resistance and enterotoxigenicity of escherichia coli.three groups of five piglets were formed and 1390 escherichia coli isolates were obtained during the 45-day period of observation. one of the groups received feed without antibiotic whereas the second received feed containing 100 ppm neomycin and the third feed with 100 ppm neomycin plus 100 ppm tetracycline. rectal swabbings for bacterial isolation were repeated ten times, twice during an adaptation period and eight times during the treatment period. resistance among the isolates to tetracyclin ...19806994861
the effects of laboratory animal diets on the potency tests of bacterial vaccines.comparative studies of the responses elicited by mice fed on pcd and ffg diets to a number of bacterial vaccines have shown a significant reduction in the immune response to tetanus toxoid but not to clostridium septicum toxoid and increased resistance to challenge with e. coli and syngeneic tumour cells but not to pasteurella multocida. these differences cannot readily be explained in terms of differences between the identifiable constituents of the diets and illustrte the dangers of vaccine po ...19806995215
identification of a carbohydrate receptor recognized by uropathogenic escherichia coli.earlier investigations have shown that pyelonephritic escherichia coli specifically recognize and bind to carbohydrate structures correlated to the p blood group antigens. these findings are confirmed and extended in this study. twenty-two of 23 nonselected e. coli strains from children with acute febrile pyelonephritis failed to agglutinate human erythrocytes lacking the antigens within the p blood group system. only one of 32 faecal isolates exhibited this specific agglutinating property. the ...19806997213
persistence and distribution of erysipelothrix rhusiopathiae and bacterial indicator organisms on land used for disposal of piggery effluent. 19806997256
effects of iron on the anti-coli capacity of sow's milk in vitro and in ligated intestinal segments.the effect of iron on the ability of sow's milk to suppress escherichia coli was examined both in vitro and in ligated intestinal segments of neonatal pigs. sow's milk from day 7 of lactation was diluted with nahco3/kh2po4 and divided into four treatment groups: (1) diluted milk; (2) diluted milk + ferric citrate; (3) diluted milk heated at 100 degrees for 20 minutes, and (4) treatment 3 + ferric citrate. treatment groups were tested for antibacterial activity in vitro after inoculation of 10(4) ...19806997445
[prospects for production of improved preparations of preventing infectious diseases]. 19806998226
[escherichia coli r live vaccine suicolplex "dessau"].immunisation of pregnant sows prior to parturition has long proved to be a good method to forestall coli dysentery in piglets before weaning. inactivated vaccines of the pathogenetically important e. coli serogroups with and without adjuvant so far were primarily used at international level. a vaccine of that kind has become available in the gdr more than eight years ago. its name is coliporc "dessau". a live vaccine has been developed from two r-mutants at the authors' institute. the effectiven ...19806998398
cephamycin c treatment of induced enterotoxigenic colibacillosis (scours) in calves and piglets.cephamycin c is a beta-lactam antibiotic that has broad gram-negative activity and is resistant to degradation by beta-lactamases and safe for use in animals. in colostrum-fed calves infected with escherichia coli strain b44, cephamycin c administered by gavage at 31.3 to 1,000 mg per calf (0.75 to 24 mg/kg) twice a day for 6 days starting at 20 h post-inoculation eliminated the diarrhea and reduced the mortality from 90% in infected, nonmedicated calves to 14% in infected, medicated calves (p < ...19806999985
[experiences with the e. coli vaccines ecopig and porcovac]. 19807004435
[biochemical investigations on the blood serum of piglets with colibacillosis (edema disease) (author's transl)]. 19807006964
the recovery of sublethally injured escherichia coli from frozen meat. 19807007308
sensitivities of escherichia coli isolated from bovine and porcine enteric infections to antimicrobial antibiotics.the antibiotic sensitivities of escherichia coli isolated from 91 diarrheal pigs and 98 diarrheal calves were determined to 19 different microbials by the kirby-bauer method. virtually all e coli isolated from pigs and calves were sensitive to amikacin, colistin, gentamicin, and polymyxin b. in vitro minimum inhibitory concentrations for these 4 antibiotics were determined for isolates from both pigs and calves.19807011124
[the protective effect of an edta-sodium-extract-vaccine obtained from swine-pathogenic e. coli in a field trial. 2. communication: immunization of weanling piglets (author's transl)].pentavalent edta-sodium-extract vaccines and monovalent edta-sodium-extract vaccines obtained from swine-pathogenic e. coli were used to test their antiepizootic effect in industrial pig farms, 2929 piglets after weaning were included in this field trial, 1309 were immunized partly orally, partly i.m., 1620 piglets received placebos accordingly. by means of oral immunization the pentavalent vaccine was capable of reducing the morbidity of the vaccinated animals significantly. intramuscular immun ...19806775450
studies on the predisposing role of stress in the e. coli-diarrhoea of pigs. 19807013371
prevalences of various bioactive escherichia coli strains isolated from clinical materials in tokyo area in 1978. 19807024609
escherichia coli vaccine for prevention of neonatal enteric colibacillosis in pigs. 19807018203
evidence of species specificity in the cytocidal effects of pasteurella haemolytica.pasteurella haemolytica was found to have a cytotoxic effect on the peripheral blood mononuclear leukocytes of cattle, sheep, and goats, but no effect on the mononuclear leukocytes of swine, horses, or humans. in contrast, escherichia coli caused marked cell death among mononuclear leukocytes of all species tested.19807439998
[model for in vivo study of antibiotics efficacy and level of escherichia coli implantation in the digestive tract (author's transl)]. 19817044257
dietary manipulation of gastric ph in the prophylaxis of enteric disease in weaned pigs: some field observations. 19817039079
a method for the intraocular injection of micro-volumes in small animal species.a method has been developed for the intraocular administration of small volumes (0.5-1.5 microliters) in small animal species such as guniea pigs and rats. the method was found to yield reproducible injection volumes and resulted in minimal ocular trauma. the aqueous humor of eyes injected with saline contained normal levels of protein and was free of white blood cells. responses to prostaglandin e2 and e.coli endotoxin were dose-dependent and as expected.19817049581
synergistic interaction of bactericidal drugs and a factor present in serum ultrafiltrate.ultrafiltrate of serum of guinea-pig and man contains a factor which interacts synergistically with cephaloridine, kanamycin, and nalidixic acid against escherichia coli. the factor on its own is not bactericidal. gel filtration showed that the main peaks of the fractions with biological activity correspond to a mw of about 200. chemical analysis excluded the presence of carbohydrates, proteins, ribonucleic acid at levels detectable with standard methods. moreover the activity of the factor was ...19817018501
polyserositis and meningitis associated with escherichia coli infection in piglets.two piglets which had a history of anorexia and weakness were examined pre and postmortem. other piglets in the same herd had died within 24 hours of the onset of similar signs. the two piglets examined had a fibrinous polyserositis. grossly, the pleura, peritoneum and joints were affected and an acute meningitis was noted on microscopic examination of the brains. pure cultures of escherichia coli were recovered from all but one of the organs and exudates cultured.19817018661
Displaying items 301 - 400 of 1980