Publications
Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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human monoclonal antibodies. | 1984 | 6087967 | |
t-cell-mediated regression of "spontaneous" and of epstein-barr virus-induced b-cell transformation in vitro: studies with cyclosporin a. | the regression of epstein-barr (eb) virus-transformed b-cell outgrowth which is seen in experimentally-infected cultures of blood mononuclear (um) cells from healthy seropositive donors can be abolished in medium containing the t-cell-suppressive agent cyclosporin a (csa) at concentrations of 0.05 microgram/ml and above. csa mediates its effect within the first 4 days post-infection of the um cells and this prevents subsequent in vitro generation of the eb virus-specific cytotoxic-t-cell respons ... | 1984 | 6088089 |
immune responses in cardiac transplantation. i. detection of activated tia+ cells in the blood during herpes virus infections. | lymphocyte subpopulations in the blood of cardiac transplant recipients were monitored by single and double marker rosetting tests; using monoclonal antibodies to monomorphic determinants on t cells and 'ia' antigens. elevated absolute numbers and percentages of tia+ cells were found in association with primary cytomegalovirus (cmv), and reactivation of epstein-barr virus or herpes simplex virus. serial tests showed that in primary cmv tia+ cells peaked before the maximal igm and igg anti-viral ... | 1984 | 6088138 |
factors influencing the human cytotoxic t cell response to autologous lymphoblastoid cell lines in vitro. | epstein-barr virus (ebv)- and fetal calf serum (fcs)-specific cytotoxic human t cells can be generated in vitro, and have been shown to be hla-antigen restricted. in the present work, peripheral blood mononuclear cells were stimulated with the gamma-irradiated autologous lymphoblastoid cell line (lcl) grown previously in fcs, human serum, or without serum. the induction and generation of cytotoxic t cells was carried out exclusively in culture medium containing autologous serum. with ebv-seroneg ... | 1984 | 6088142 |
human leukaemic cell lines. | 1984 | 6088146 | |
early nasopharyngeal carcinoma among iga/vca antibody positive individuals detected by anticomplement immunoenzymatic method. | 1984 | 6088182 | |
acid lipase and carboxylesterases in ebv-transformed lymphoid cell line from wolman's disease: influence of fatty acid structure of substrate. | lymphoid cell lines established by epstein-barr virus transformation of blood b lymphocytes from a patient with wolman's disease exhibited the acid lipase deficiency characteristic for this disease. comparison of hydrolysis by normal and wolman's cells of 4-methylumbelliferyl-acyl esters with variable chain length demonstrates that: (1) the best substrates for acid lipase were characterized by an acyl chain length of 12-18 carbon atoms; (2) the acid residual activity in wolman's cells showed a s ... | 1984 | 6088221 |
increased serum levels of antibodies to epstein-barr virus in adults with history of atopic dermatitis. | serum antibodies to epstein-barr virus (ebv), varicella-zoster (vzv) and herpes simplex virus (hsv) were determined in 140 patients with active or healed atopic dermatitis (ad) and 48 control individuals. antibody titers against ebv were significantly higher in ad patients than in the controls irrespective of whether the ad was active or healed at the time of blood sampling. no difference in ebv antibody titers were observed between ad patients with asthma and/or hay fever and those without such ... | 1984 | 6088400 |
a population-based case-control study of ebv and other viral antibodies among persons with hodgkin's disease and their siblings. | the epstein-barr virus (ebv) has been consistently found to be associated with hodgkin's disease (hd) in two ways: cases generally have elevated titer distributions of antibodies against the viral capsid antigen, and the occurrence of hd among persons with a history of ebv infectious mononucleosis is two or three times higher than expected. we evaluated this association by measuring the prevalence and level of antibodies against ebv and related viruses among 304 cases of hd interviewed in a popu ... | 1984 | 6088401 |
type-i htlv antibody in urban and rural ghana, west africa. | the prevalence of antibodies against the newly described human t-cell lymphoma virus, type i (htlv-i) in two communities in ghana, west africa, is described. there was no difference by community (urban, 3.6% and rural, 4.0%). prevalence increased with age, being 5.9% among persons greater than 10 years old, but did not differ by sex. there was no difference when data were analysed by housing status or crowding. non-confirmed reactions in the assay system were frequent and correlated with both pr ... | 1984 | 6088402 |
infectious complications after open heart surgery. | 1984 | 6088432 | |
a human monoclonal antibody to cytomegalovirus (cmv). | we report the development of a human monoclonal antibody to cytomegalovirus (cmv) produced from a human x human hybridoma. this hybrid was developed by fusion of an ebv-transformed cell line making antibody to cmv and a human lymphoblastoid cell line wi-l2. the antibody is directed to a cmv-specific antigen primarily in the nucleus of cmv-infected human fibroblasts. it cross-reacts with at least 10 different strains of cmv and may provide a method for the rapid in vitro diagnosis of cmv infectio ... | 1984 | 6088634 |
reverse enzyme-linked immunospot assay (relispot) for the detection of cells secreting immunoreactive substances. | a reverse modification of the recently described enzyme-linked immunospot assay (elispot), based on localized enzyme-substrate reactions in gel, is described for the enumeration of antigen-secreting cells using petri dishes coated with specific antibodies. as a model the detection of mouse and human immunoglobulin-secreting cells has been evaluated. simple and sensitive, this new method, termed relispot, can be adapted for the quantitation of secreted antigen thus providing additional informatio ... | 1984 | 6088638 |
persistence of epstein-barr virus in the parotid gland. | two independent techniques, in situ hybridization on frozen sections and reassociation kinetics, have been used to localize epstein-barr virus genomes in tissue samples from healthy human adults. whereas specimens taken from the palatine tonsils were invariably negative, all samples from the parotid gland were positive when tested with either technique. this observation suggests that the parotid gland is, besides the peripheral lymphocytes, a site of lifelong persistence of epstein-barr virus an ... | 1984 | 6088802 |
tumorigenicity of nasopharyngeal carcinoma hybrid cell line. | the spontaneous production of epstein-barr virus (ebv) and tumorigenicity in the balb/c mutant nude mouse were studied with the use of the following 4 cell lines derived from the human nasopharynx: a) ad-ah, an 8-azahypoxanthine-resistant epithelioid cell line; b) a2l, an ebv-carrying lymphoblastoid cell line; c) a2l/ah, an ebv-carrying epithelioid hybrid cell line established by fusion of ad-ah cells with a2l cells; and d) npc-kt, an ebv-carrying epithelioid hybrid cell line established by fusi ... | 1984 | 6088881 |
recovery of epstein-barr virus from genital ulcers. | 1984 | 6088983 | |
complications of infection with epstein-barr virus during childhood: a study of children admitted to the hospital. | we undertook a study of infection with epstein-barr virus (ebv) in order to ascertain the spectrum of complications from this disease. during a 6-year period, 51 children were admitted; review of the 50 available records confirmed the diagnosis of ebv infection in 48, 37 of whom had clinical infectious mononucleosis. the indications for admission were diagnostic evaluation in 14 (29%) children, airway obstruction in 12 (25%) children, dysphagia and dehydration in 11 (23%) children, airway obstru ... | 1984 | 6089127 |
[epstein-barr virus in human pathology (excluding infectious mononucleosis)]. | 1984 | 6089294 | |
prevalence of markers of hepatotrophic viruses in alcoholics with symptomatic liver cirrhosis or pancreatitis. | the reason why similar amounts of alcohol consumption cause different types of organ damage in alcoholics is obscure. recent studies indicate that hepatitis b virus infection may influence the development of liver cirrhosis in alcoholics. we investigated the prevalence of markers of viruses known to cause hepatitis (hav, hbv, ebv, cmv) in two groups of patients, one with alcoholic pancreatitis without known liver cirrhosis and one with alcoholic liver cirrhosis without known pancreatitis. we fou ... | 1984 | 6089303 |
studies on mechanism of entry of eb virus into non-b lymphocytes. establishment of an eb virus-producing human-tupaia hybrid cell line tuh18. | with the use of the eb virus-producing lymphoblastoid cell line h18, an attempt was made to infect the monolayer cell culture of nasopharyngeal tissue from the normal tupaia, and an eb virus-producing, human-tupaia hybrid cell line was established. the mechanism of entry of eb virus into cells other than b lymphocytes is discussed in this paper. | 1984 | 6089329 |
effect of an anticancer agent, vinblastine, on mouse skin. | the effect of vinblastine (vlb), an anticancer agent which has recently been shown to have epstein-barr virus (ebv)-activating potency, on cd-1 mouse skin was investigated. a single topical application of either 15 or 100 micrograms of vlb induced epidermal hyperplasia. twice weekly applications of these doses of vlb either for 29 weeks after a 7,12-dimethylbenz(a)anthracene (dmba) initiation (one-stage promotion) or for 27 weeks after dmba initiation and a limited treatment with 12-o-tetradecan ... | 1984 | 6089384 |
an ebna-negative, ebv-genome-positive human lymphoblast cell line in which superinfecting ebv dna is not maintained. | a human b-lymphoid cell line, designated tg8, which does not express detectable levels of the ebv (epstein-barr virus)-associated nuclear antigen (ebna), yet carries an average of one to two plasmid copies of the p3hr-1 ebv genome has been identified. tg8 can be superinfected by b95-8 ebv, resulting in up to 60-70% of the population becoming ebna-positive and 20-30% of the incoming ebv genomes becoming circular by 48 hr postinfection. neither ebna expression nor the superinfecting viral dna is m ... | 1984 | 6089410 |
epstein-barr virus (p3hr-1) defective dna codes for components of both the early antigen and viral capsid antigen complexes. | a set of lambda phages containing overlapping fragments of epstein-barr virus (ebv) defective dna has been cloned from p3hr-1-superinfected raji cells. mapping data obtained using these cloned dna fragments confirmed the structure of p3hr-1 defective dna previously deduced directly from virion dna (m.-s. cho, g. w. bornkamm, and h. zur hausen, 1984, j. virol., in press). the ability of the cloned defective dna fragments to induce ebv antigens in transfected baby hamster kidney (bhk) cells was te ... | 1984 | 6089423 |
[lymphatic diseases specific to the tropics (burkitt's lymphoma, tropical splenomegaly and mediterranean lymphoma)]. | certain lymphoproliferative disorders (burkitt's lymphoma, tropical splenomegaly, and mediterranean lymphoma) are more frequently observed in the tropical or subtropical areas than in other parts of the world. the polymicrobial exposure of human beings in those areas to viral, bacterial and protozoal infections led to the assumption of a specific connection between these conditions and the lymphoproliferative disease. some evidence has accumulated that the epstein-barr virus might be an oncogeni ... | 1984 | 6089444 |
abnormal b-cell proliferation associated with combined immunodeficiency, cytomegalovirus, and cultured thymus grafts. | a male infant in whom multiple recurrent multiorgan infections developed during the first six months of life was found to have combined immunodeficiency. progressive pulmonary disease developed at age two years; cytomegalovirus (cmv) was isolated from the respiratory tract and urine. three separate intramuscular grafts of cultured thymus fragments did not produce change in the course of the illness. soon after age three years, igg lambda appeared in the serum as an m-component. the patient died ... | 1984 | 6089543 |
[nasopharyngeal carcinoma: clinical and therapeutic aspects. i. introduction and symptomatology]. | 1984 | 6089603 | |
acyclovir: an update of the clinical applications of this antiherpes agent. | this paper reviews the clinical evaluation of acyclovir in the treatment of herpes-virus infections, predominantly those due to herpes simplex and varicella-zoster viruses. intravenous, oral and topical acyclovir have been reported to be effective in the therapy of a wide variety of established herpes simplex virus infections and the systemic drug has been shown to be capable of suppressing reactivation of that virus. although acyclovir has less activity against varicella-zoster virus, infection ... | 1984 | 6089659 |
effects of n-butyrate and phorbol ester (tpa) on induction of epstein-barr virus antigens and cell differentiation. | n-butyrate, an effective inducer of synthesis of epstein-barr virus (ebv) antigens in virus-producer p3hr-1 cells, has recently been shown (2) to induce morphological differentiation towards plasma cell in nonproducer raji cells. the effects of n-butyrate and 12-o-tetradecanoylphorbol-13-acetate (tpa) on both ebv-antigen induction and cell differentiation in two virus-nonproducer lymphoblastoid cell lines, raji and nc37, were now studied. the following observations were made (1). on its own eith ... | 1984 | 6089703 |
further characterisation of a human monoclonal antibody to the rhesus d antigen produced in vitro. | uch d4 is a human monoclonal antibody produced by an epstein-barr (eb) virus-transformed lymphoblastoid cell line. the antibody is specific for the rhesus d antigen, and is a good laboratory reagent for red blood cell typing. uch d4 can be purified from the culture supernatant medium with the removal of all detectable dna and infectious eb virus particles. this purified antibody will be used for in vivo assessment of its ability to prevent rhesus disease of the newborn. | 1984 | 6089730 |
establishment of t and b cell lines from patients with mycosis fungoides. | eighteen patients with mycosis fungoides were studied in order to establish cell lines that might be associated with the human t cell leukaemia-lymphoma virus (htlv). three t cell lines were established, two from affected skin and one from a lymph node showing dermatopathic lymphadenopathy. the t cells expressed okt3 and okt4 antigens. they temporarily expressed an htlv p19-like antigen in up to 5% of the cells during culture. none of our patients had lymphocytosis or abnormal lymphocytes, excep ... | 1984 | 6089859 |
determination of the internal volume of reconstituted sendai virus envelopes by quenching of calcein fluorescence. | sendai virus envelopes (sve) were isolated from sendai virus particles by triton x-100 solubilization and ultracentrifugation. the envelopes were reconstituted in the presence of the fluorescent dye calcein by gradual removal of the detergent with bio-beads sm-2. the internal volume of reconstituted sendai virus envelopes (rsve) was determined by quenching the fluorescence of calcein with cobalt (ii) ions. the internal volume of rsve was found to be proportional to the initial sve protein concen ... | 1984 | 6089930 |
arh-77, an established human igg-producing myeloma cell line. i. morphology, b-cell phenotypic marker profile, and expression of epstein-barr virus. | monolayer cultures of arh-77 cells, a human myeloma cell line propagated in vitro, display a variety of morphologic entities ranging from small lymphocytes to classic plasma cells. the cells show intense pyronin and periodic acid-schiff affinity but are negative for colloidal iron, sudan black, and naphtol as-d chloroacetate esterase. the cells exhibit phenotypic markers pertaining to each stage of the b-cell lineage. they fail to display sheep erythrocyte and bovine erythrocyte-igg antibody com ... | 1984 | 6090003 |
activated oncogenes in burkitt's lymphoma. | 1984 | 6090072 | |
spliced rna from the ir1-u2 region of epstein-barr virus: presence of an open reading frame for a repetitive polypeptide. | we have constructed a cdna library from the cytoplasmic rnas of raji cells, a burkitt's lymphoma cell line latently infected with epstein-barr virus. we report here the characterization of a cdna representing a spliced rna transcribed from the ir1-u2 region of the viral genome. the cdna is 1007 bp long. the 5' region contains three tandem repeats of two exons, 66 and 132 bp, which are transcribed from the ir1 repeats. the 3' region is formed from four exons transcribed from u2. an open reading f ... | 1984 | 6090131 |
[effects of anti-mb and anti-dr monoclonal antibodies on in vitro immune responses]. | the present study has addressed the effects of anti-dr and anti-mb monoclonal antibodies on various in vitro immune responses. the anti-dr monoclonal antibody hu-4 was found to inhibit t cell proliferative responses to ppd, autologous non-t cells, autologous eb-virus-transformed b cells, and alloantigens. furthermore, hu-4 strongly inhibited generation of killer t cells specific for autologous eb-virus-transformed b cells and alloantigens. in contrast, the anti-mb monoclonal antibody hu-18 did n ... | 1984 | 6090290 |
paired epstein-barr virus-carrying lymphoma and lymphoblastoid cell lines from burkitt's lymphoma patients: comparative sensitivity to non-specific and to allo-specific cytotoxic responses in vitro. | paired epstein-barr (eb) virus-carrying cell lines have been established from burkitt's lymphoma (bl) patients; one the bl-cell line derived from the malignant cells of the tumour and bearing the relevant chromosomal translocation, the other the diploid lymphoblastoid cell line (lcl) derived from the patient's normal b cells by experimental infection with the virus. comparative studies have shown that both bl and lcl cells are relatively resistant to fresh nk cells but are more susceptible to in ... | 1984 | 6090321 |
molecular size variation of ebna is determined by the eb viral genome. | the size classes of the epstein-barr virus(ebv)-induced nuclear antigen (eb-na) have been determined in ebv-carrying sublines of two originally ebv-negative burkitt lymphomas, converted in vitro by two different ebv substrains, and in somatic hybrids between two ebv-carrying cell lines or between an ebv-positive and an ebv-negative line. partially purified ebna components were detected by western blotting and subsequent exposure to antiserum/alkaline-phosphatase-coupled protein a complexes. conv ... | 1984 | 6090339 |
hla loss variants of a b27+ lymphoblastoid cell line: genetic and cellular characterization. | variants of a lymphoblastoid cell line, lcl 526 (sb3 mb1 dr1 b44 c5 a2/sb4 mt4 dr4 b27 c2 a24), which lost various hla specificities were selected with monoclonal antibodies and complement using a method developed by kavathas et al. (pnas 77:4251, 1980). using alpha b27 monoclonals, 8 b27 only loss mutants and 4 b27 haplotype multiple loss mutants were generated. the parental lcl 526 and two of the b27- mutants were used to select alpha b27 ctls. the selection of six a2 loss, one a2-c5 loss, and ... | 1984 | 6090351 |
identification of multiple epstein-barr virus-induced nuclear antigens with sera from patients with rheumatoid arthritis. | by means of the protein immunoblot technique, the epstein-barr virus (ebv) nuclear antigen (ebna) could be identified in a variety of ebv-transformed cell lines with anti-ebna-positive sera from normal donors. the molecular weight of ebna expressed in each of the cell lines varied between 70,000 and 75,000 and was dependent upon the strain of infecting virus. in contrast, 15 of 21 sera from patients with rheumatoid arthritis identified antigens in addition to ebna. the most prominent of these an ... | 1984 | 6090712 |
a case of japanese burkitt's lymphoma associated with eb virus. | a 6-year-old girl was admitted for cervical lymphadenopathy. she was diagnosed histologically as having burkitt's lymphoma with partial involvement of follicles. immunological study of a biopsy specimen demonstrated that many cells were surface immunoglobulin (lambda light chain)-positive, and chromosomal analysis revealed a translocation of (8;14) (q24;q32) in all blastic cells. her serum antibody titers to epstein-barr virus (ebv) capsid antigen and to early antigen were 1:320 and 1:160, respe ... | 1984 | 6090736 |
establishment of lymphomatous cell lines from bone marrow samples from patients with burkitt's lymphoma. | a total of 233 bone marrow aspirates were obtained from 43 patients with burkitt's lymphoma (bl). lymphoma cells were absent and lymphoblastoid cell lines could not be established from 197 samples, which were characterized by limited initial cell proliferation and development of an adherent population, followed by cell death after 2-4 weeks. in 14 aspirates, after a similar pattern of growth, cell proliferation began again after about 6 weeks, with a rapid appearance and growth of cell clumps fr ... | 1984 | 6090749 |
defective immune surveillance in viral carcinogenesis. | 1984 | 6090808 | |
epstein-barr-virus induced lymphoproliferation. implications for antiviral chemotherapy. | 1984 | 6090906 | |
expression of epstein-barr virus (ebv) dna and cloned dna fragments in human lymphocytes following sendai virus envelope-mediated gene transfer. | purified ebv dna and cloned dna fragments were trapped in sendai virus (sv) envelopes during envelope reconstitution. the dna-loaded reconstituted envelopes (rsve/dna) served as gene-transfer vehicles using the capability of rsve to fuse with normal and tumor cells. the efficiency of rsve-mediated ebv dna transfer into lymphoid tumor cells and fresh human lymphocytes was 5-10% of the enveloped 3h-labeled ecori fragment b of ebv dna. purified intracellular ebv (b95-8 strain) dna induced ebv nucle ... | 1984 | 6091119 |
lymphoblastoid cell-induced suppression of human peripheral blood leukocyte mitogenic responses. | two lymphoblastoid tumor cell lines, the burkitt lymphoma derived bjab cell line which is free of epstein-barr virus (ebv) and b95-8 cells, which are marmoset lymphocytes transformed by ebv isolated from an infectious mononucleosis patient, were studied in regards to their effects on the blastogenic responsiveness of normal human peripheral blood leukocytes stimulated in vitro with mitogens. mitomycin c treated tumor cell suspensions, when cocultured with normal human blood leukocytes, markedly ... | 1984 | 6091146 |
grand rounds: pathogenesis of infections mononucleosis. | 1984 | 6091358 | |
[virologico-serologic studies in patients with brain tumors]. | the etiological role played by human pathogenic herpes viruses for diseases of the central nervous system has been verified (herpes simplex virus, varicella zoster virus) or is being discussed (cytomegalo virus, epstein-barr virus). in this study, the significance of herpes virus infections for surgically treated cerebral tumour patients is examined. in a total of 30 patients with various cerebral tumours, infectious virus from cerebral tumour tissue could be isolated in none of the cases. the m ... | 1984 | 6091370 |
[post-infectious epstein-barr virus encephalitis]. | a case of meningoencephalitis is reported in a 14 year old boy. after an acute onset with fever and coma, the outcome was rapidly favorable. antibody titers in acute and convalescent sera demonstrated recent infection with ebv. ebv encephalitis appears to be a post-infectious encephalitis with no direct viral invasion of the cns and no acute viral replication. | 1984 | 6091587 |
application of flow cytometry to studies on the distribution, persistence, and regeneration rate of epstein-barr virus receptors. | flow cytometry has been applied to study the persistence and regeneration rate of epstein-barr virus (ebv) receptors and other membrane proteins in normal and tumor cells. ebv receptors were detected in a binding assay utilizing fluorescein isothiocyanate-conjugated virions (fitc-ebv). after stripping receptors from the cell surface with trypsin, binding of fitc-ebv was rapidly regenerated in the lymphoma cell line loukes, reaching 75% of the control levels by 3 h. fresh human lymphocytes regene ... | 1984 | 6092014 |
epidemiology of hodgkin's disease. | hodgkin's disease displays an intriguing variation in incidence with age. for adult disease, there is striking bimodality in incidence with peaks in young adulthood and in older adulthood. on epidemiologic grounds, there appear to be three different diseases: childhood (0-14 years), young adult (15-34 years), and older adult hodgkin's disease (55+ years). there are marked geographic variations in hodgkin's disease incidence, but the variations are different for the three age groups. there are al ... | 1984 | 6092122 |
establishment and characterization of adult t-cell leukemia virus-containing b-cell lines derived from peripheral blood of adult t-cell leukemia patients. | b-cell lines which showed predominantly surface iga kappa immunoglobulins were established from each of seven adult t-cell leukemia (atl) patients. atl virus proviral dna was found in five of the lines. though the chromosomal analysis of the b-cell lines yielded normal results, fresh leukemic t-cells showed chromosome abnormalities. | 1984 | 6092185 |
[epstein-barr virus infection and immune responses in primary immunodeficiency syndromes]. | 1984 | 6092247 | |
low secretory iga concentrations in oral secretions during the acute phase of infectious mononucleosis. | although the epstein-barr virus (ebv) relation to infectious mononucleosis (im) has been well established, the question why im is not compulsory during a primary ebv infection has yet to be solved. assuming a possible oropharyngeal secretory immunoincompetence as an etiological component of im, the secretory iga concentrations in oral secretions during the acute phase of im was investigated. low concentrations of secretory iga in im patients (n = 18) were found, mean value 0.180 g/l as compared ... | 1984 | 6093236 |
chronic mononucleosis syndrome. | we present data on 14 patients with chronic symptoms of disabling fatigue in association with serologic evidence of active epstein-barr virus (ebv) infection. two thirds were women, and the average age at onset was 29.6 years. forty-three percent were known to have had previous infectious mononucleosis, but the usual criteria for that diagnosis were not helpful with the present syndrome. eighty-six percent had serologic evidence of cytomegalovirus (cmv) infection. profound immunodeficiency was n ... | 1984 | 6093268 |
the effect of cytomegalovirus and epstein-barr virus infection on t lymphocyte subsets in cardiac transplant patients on cyclosporine. | 1984 | 6093294 | |
use of epstein-barr virus to transform fetal human spleen cells for hla typing. | 1984 | 6093295 | |
identification of epstein-barr virus genes expressed during the early phase of virus replication and during lymphocyte immortalization. | transcription of the epstein-barr virus (ebv) genome in raji cells superinfected with p3hr-1 ebv in the presence of cycloheximide was compared to transcription in human lymphocytes infected with transforming ebv (b95-8). this was done to identify regions of the ebv genome which contain genes that may mediate initiation of virus replication. hybridization of 32p-labeled cdna to cloned fragments of ebv dna (dot blot hybridization) was employed to identify transcriptionally active regions of the vi ... | 1984 | 6093376 |
hypothesis: the pathogenesis of aids. activation of the t- and b-cell cascades. | the hypothesis is presented that a human t-cell leukemia virus (htlv) or a related agent produces a lytic response of t cells manifested by the acquired immune deficiency syndrome (aids) and a proliferative response represented by the adult leukemia/lymphoma (atl) syndromes. the sequence or cascade of t-cell events following loss of t4 helper cells in aids includes reactivation of epstein-barr virus and a b-cell cascade of cytomegalovirus, resulting in kaposi sarcoma in genetically susceptible p ... | 1984 | 6093395 |
differentiation of chronic lymphocytic leukemia cells after in vitro treatment with epstein-barr virus or phorbol ester. i. immunologic and morphologic studies. | leukemic cells from ten patients with "nonsecretory," b-type chronic lymphocytic leukemia (cll) were cultured alone or in the presence of epstein-barr virus (ebv) or a phorbol ester (12-0-tetradecanoylphorbol-13-acetate; tpa) for 7 days. at periodic intervals the cell morphology, cytoplasmic immunoglobulin content (direct immunofluorescence) and capacity for immunoglobulin secretion (hemolytic plaque assay) were assessed. a variable but significant number of the tpa-treated cll cells from all pa ... | 1984 | 6093504 |
nih conference. rheumatoid arthritis: evolving concepts of pathogenesis and treatment. | rheumatoid arthritis is a chronic inflammatory synovitis that primarily involves peripheral diarthrodial joints. immunohistologic analysis of diseased synovium has shown a spectrum of abnormalities that resemble various stages of a cell-mediated, or delayed-type, immune reaction. the infiltrating mononuclear cells produce various factors that modulate adjacent tissues and appear to produce the characteristic destructive features of the disorder. our understanding of the mechanisms of action of v ... | 1984 | 6093663 |
antibodies to epstein-barr virus in patients with nasopharyngeal carcinoma and in comparison groups. | one hundred fifty-one patients with nasopharyngeal carcinoma (npc) were enrolled in a prospective collaborative study of north american patients (most were white). serum samples were collected concurrently from 903 mayo patients for comparisons of serologic analyses. both iga antiviral capsid antigen [vca (iga)] and the anti-early antigen [ea (d)] antibody responses were associated with diagnosis, but not always in the same way. both responses were positive in 85% of patients with world health o ... | 1984 | 6093669 |
lytic, nontransforming epstein-barr virus (ebv) from a patient with chronic active ebv infection. | a new wild-type isolate of epstein-barr virus (ebv) was identified in follow-up studies of a case of chronic active ebv infection in an 8-year-old girl who had high titres of antibody to viral capsid antigen and early antigen (ea) (greater than 20 480 and 2560 respectively), persistent splenomegaly and abnormal immunologic features. more than 10 throat washings from this patient failed to transform cord blood lymphocytes (cbl), but at least 7 were able to induce ea in raji cells. supernatants fr ... | 1984 | 6093976 |
the concurrence of saethre-chotzen syndrome and malignancy in a family with in vitro immune dysfunction. | the occurrence among 13 siblings of a malformation-mental retardation syndrome and diverse malignancies was investigated for etiologic relationship by clinical, genetic, immunologic, and virologic techniques. three sisters and their father had saethre-chotzen syndrome, an autosomal dominant trait with craniosynostosis and asymmetric facies. one affected sister also had nasopharyngeal carcinoma; tow nondysmorphic brothers had hodgkin's disease, and another had seminoma with teratocarcinoma of the ... | 1984 | 6093987 |
production of autoantibodies to cellular antigens by human b cells transformed by epstein-barr virus. | lymphocytes obtained from the blood of three patients who lacked clinical and serologic evidence of autoimmunity were inoculated with epstein-barr virus (ebv) and plated in microwell cultures at low cell densities. large numbers of the resulting transformed cell lines produced igm autoantibodies which reacted by elisa with antigens in cultured human fibroblasts. precursor frequencies of autoantibody-secreting transformed cells ranged from 27 to 630 per 10(6) cells. autoantibodies from 20 elisa-p ... | 1984 | 6094060 |
herpes virus infections: clinical manifestations and therapeutic strategies in immunocompromised patients. | herpesvirus infections are a major cause of morbidity and mortality in immunosuppressed patients. bone marrow and organ transplant recipients are model patient populations for studying the natural history of herpesvirus infections because the infections occur predictably after transplantation. herpes simplex virus infections occur within the first month after transplantation and may cause severe mucocutaneous disease. cytomegalovirus infections occur one to three months after transplantation and ... | 1984 | 6094062 |
t-lymphocyte t4 molecule behaves as the receptor for human retrovirus lav. | many viruses, including retroviruses, are characterized by their specific cell tropism. lymphadenopathy-associated virus (lav) is a human lymphotropic retrovirus isolated from patients with acquired immune deficiency syndrome (aids) or related syndromes, that displays selective tropism for a subset of t lymphocytes defined by the expression of a surface glycoprotein of relative molecular mass 62,000 (62k) termed t4 (refs 6-8). this glycoprotein delineates a subset of t lymphocytes with mainly he ... | 1984 | 6083454 |
[a monoclonal antibody, specific for burkitt's lymphoma, is also a blood group pk antibody]. | we have previously described a monoclonal antibody, referred to as 38.13, reacting with most burkitt's lymphoma (bl) derived lines, either containing the epstein-barr virus (ebv) or not, but not reacting with ebv-positive lymphoblastoid cell lines (lcl). (pnas 78: 6485, 1981; int. j. cancer 29: 653, 1982). the antibody reacted with 41 bl lines out of 57 tested so far. the target antigen. bla, was shown to be a neutral glycolipid, identified as globotriaosylceramide (gal alpha 1----4 gal beta 1-- ... | 1984 | 6084615 |
in vitro synthesis of ige by human lymphocytes. iii. ige-potentiating activity of culture supernatants from epstein-barr virus (ebv) transformed b cells. | seven epstein--barr virus (ebv)-transformed b cell lines were derived from circulating lymphocytes of two atopic and two non-atopic individuals, two preparations of cord blood lymphocytes and one tonsillar lymphocyte preparation. all the cell lines contained a significant proportion of cells expressing fc epsilon r as detected by rosette formation with ige-coated bovine erythrocytes (e-ige) and by flow cytometry using ige-linked to fluorescent microspheres. none of the cell lines displayed fcr f ... | 1984 | 6092268 |
homozygous deletions that simultaneously eliminate expressions of class i and class ii antigens of ebv-transformed b-lymphoblastoid cells. i. reduced proliferative responses of autologous and allogeneic t cells to mutant cells that have decreased expression of class ii antigens. | mutant human b-lymphoblastoid cell lines, 721.174 and 721.180, that have greatly reduced expressions of known class i and class ii hla antigens were produced by two cycles of gamma-ray mutagenesis followed by selection for hla antigen loss. residual binding of monoclonal antibodies directed against class ii antigens was negligible except for 10% residual binding of sb-binding antibody ilr1. however, deletion of sb was functionally complete as indicated by failure of the mutants to stimulate prol ... | 1984 | 6092300 |
the antiviral spectrum of (e)-5-(2-bromovinyl)-2'-deoxyuridine. | the antiviral activity spectrum of (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) is not restricted to herpes simplex virus type 1 (hsv-1) and varicella-zoster virus (vzv) but also encompasses several other herpesviruses such as suid herpesvirus type 1 (shv-1), bovid herpesvirus type 1 (bhv-1), simian varicella virus (svv), herpesvirus saimiri, herpesvirus platyrrhinae, and the baculovirus trichoplusia ni multiple nuclear polyhedrosis virus. other herpesviruses such as herpes simplex virus type 2, ... | 1984 | 6092320 |
epstein-barr virus (ebv)-specific cell-mediated and humoral immune responses in ataxia-telangectasia patients. | as a part of studies on cell-mediated immune (cmi) responses of immunocompromised, epstein-barr virus (ebv)-infected patients who can or cannot restrict the proliferation of ebv-transformed b cells, we have studied 16 turkish patients with ataxia-telangectasia (at). fifteen were ebv seropositive; one was seronegative. among the seropositives, eight had no or only low anti-ebv-determined nuclear antigen (ebna) antibody titers, while seven had normal anti-ebna levels. ebv-seropositive and -seroneg ... | 1984 | 6092413 |
interferon production in primary immunodeficiencies. | alpha- and gamma-interferon (ifn) production by peripheral blood mononuclear cells (pbmc) from 18 patients affected by primary immunodeficiency syndromes was examined and compared with that of 20 normal donors. patients included 8 with common variable immunodeficiency (cvi), 2 with congenital agammaglobulinemia, 4 with ataxia-telangiectasia, 2 with hyper-ige syndrome, 1 with chronic ebv infection, 1 with combined immunodeficiency, and 1 with immunodeficiency with hyper-igm. no spontaneous ifn pr ... | 1984 | 6092414 |
use of a simple separation column in detection of immunoglobulin m antibody to epstein-barr virus. | the use of a new commercial separation column on whole test serum improved the method for detecting immunoglobulin m antibody to epstein-barr virus. the efficiency of this product in absorbing interfering immunoglobulin g was similar to that of another absorption product, staphylococcal protein a, but it has the advantage of being stable without refrigeration. | 1984 | 6092420 |
a human hybrid myeloma for production of human monoclonal antibodies. | we produced somatic cell hybrids between human myeloma cells and a lymphoblastoid cell line that is hypoxanthine phosphoribosyl transferase-deficient and ouabain-resistant. these hybrids were phenotypically similar to the human myeloma parental cells and grew as well as the human lymphoblastoid parental cells. after counterselection in 6-thioguanine, mutants that were 6-thioguanine-and ouabain-resistant were obtained, one of which was used as a fusion partner with lymphoblastoid b cells that pro ... | 1984 | 6092464 |
b cell lines as models for inherited phagocytic diseases: abnormal superoxide generation in chronic granulomatous disease and giant granules in chediak-higashi syndrome. | epstein barr virus (ebv)-transformed b cell lines (bcl) were developed from peripheral blood lymphocytes of individuals with chediak-higashi syndrome (chs) and chronic granulomatous disease (cgd), and were compared to ebv bcl from normals. the cells that were studied expressed both b cell surface antigens and ebv nuclear antigens. bcl from normals contained small cytoplasmic granules. when stimulated with phorbol myristate acetate (pma), these b cells reduced nitroblue tetrazolium (nbt) and gene ... | 1984 | 6092465 |
cellular origins of human polymeric and monomeric iga: intracellular and secreted forms of iga. | intracellular and secreted iga from pokeweed mitogen (pwm)-stimulated normal peripheral blood lymphocytes, from 12-o-tetradecanoylphorbol-13-acetate (tpa)-stimulated peripheral blood lymphocytes of a patient with chronic lymphocytic leukemia (cll), or from an iga-producing human epstein barr virus (ebv)-transformed lymphoblastoid cell line were analyzed by molecular-sieve chromatography, electrophoresis in sodium dodecyl sulfate, and sucrose density ultracentrifugation. fluorochrome-labeled anti ... | 1984 | 6092467 |
infectious mononucleosis: a polyclonal b cell transformation in vivo. | we obtained spontaneous formation of epstein-barr virus-transformed b lymphocyte colonies from the blood of four patients with mononucleosis with the use of soft-agar medium. the colonies were propagated into separate cell lines and analyzed for their immunoglobulin secretion. of 52 such lines, 44 produced immunoglobulin composed of a single class of heavy chain and single type of light chain. among these clonal transformants, 27 (62%) of 44 produced mu-chain, 13 (29%) of 44 produced gamma-chain ... | 1984 | 6092482 |
[antibodies to rheumatoid arthritis nuclear antigen (rana) in patients with rheumatoid arthritis]. | 1984 | 6092497 | |
expression of interleukin 2 receptors on activated human b cells. | using anti-tac, a monoclonal anti-interleukin 2 (il-2) receptor antibody, we have explored the possibility that certain activated b cells display receptors for il-2. resting normal b cells and unselected b cell lines established from normal individuals were tac antigen negative. in contrast, the cell surface tac antigen expression was demonstrable on 6 of 10 b cell lines from patients with burkitt's lymphoma, 5 of 6 b cell lines derived from patients with htlv-i-associated adult t cell leukemia ... | 1984 | 6092511 |
detection and functional studies of p60-65 (tac antigen) on activated human b cells. | a monoclonal antibody, at-1, is shown to precipitate a p60-65 molecule identical to the tac antigen. with at-1, the expression of il-2 receptors by normal activated human b cells from peripheral blood and tonsils is documented by biosynthetic and immunofluorescence studies. at-1 precipitated a p60-65 protein from [35s]methionine-labeled activated b cells, similar to that from activated t cells. the interleukin 2 (il-2) receptor appeared shortly after activation with anti-igm and b cell-stimulato ... | 1984 | 6092512 |
characterization of human b-cell lines harbouring both adult t-cell leukaemia (atl) virus and epstein-barr virus derived from atl patients. | human b-cell lines, designated atlb cell lines, were spontaneously established from peripheral blood of adult t-cell leukaemia (atl) patients. the cell lines consistently expressed atl-associated antigen (atla) and epstein-barr virus-associated nuclear antigen (ebna). a cloned atlb line, designated atlb 2, showed that both atla and ebna antigens were present in the same b-cell clone. in this study, we have further characterized atlv and ebv in the cloned atlb 2 cell line by biochemical technique ... | 1984 | 6092523 |
[demonstration of epstein-barr virus (ebv)-related antigens and tumorigenicity in nude mice in a hybrid cell line derived from the fusion of an ebv-positive lymphoblastoid cell and an ebv-negative epithelial cell from the human nasopharynx]. | 1984 | 6092593 | |
electron microscopic evidence for replication of circular epstein-barr virus genomes in latently infected raji cells. | raji cells, collected at various times from a synchronized culture, were gently lysed, and the high-molecular-weight dna was enriched ca. 10-fold for latent epstein-barr virus (ebv) genomes by equilibrium density gradient centrifugation in neutral cscl. the heavy-density dna pool, which included more than 90% of the total intracellular ebv dna sequences, was further fractionated by velocity sedimentation on neutral glycerol gradients, and material from fractions containing potential ebv dna repl ... | 1984 | 6092676 |
epstein-barr virus-transformed lymphocytes produce monoclonal autoantibodies that react with antigens in multiple organs. | peripheral blood lymphocytes from normal individuals and patients with autoimmune abnormalities such as insulin-dependent diabetes mellitus and thyroiditis were infected with epstein-barr virus, and the culture supernatants were tested for autoantibodies that reacted with normal tissues. between 58 and 86% of epstein-barr virus-transformed cultures produced immunoglobulin m antibodies, and between 9 and 24% of the transformed cultures produced immunoglobulin g antibodies that reacted with normal ... | 1984 | 6092698 |
[fluctuation in epstein-barr virus-related antibody titers by age: response differences between the igg antibodies to vca and anti-ebna antibodies]. | 1984 | 6092744 | |
epstein-barr virus-associated antibodies and serum biochemistry in nasopharyngeal carcinoma. | nasopharyngeal carcinoma is difficult to diagnose in its early stages. it also has frequent recurrences and/or distant metastases after radiotherapy. extensive clinical, serological and biochemical studies were done during 1980-1982 on 351 patients to aid in the diagnosis of the disease, especially with recurrence or metastasis. seropositive rates of the antibody titers against viral capsid antigens (vca) and early antigens (ea) of epstein-barr virus (ebv) in igg and iga classes were 41.7%-100%. ... | 1984 | 6092804 |
acquired immune deficiency syndrome. a surgical perspective. | eighty-nine lymph node biopsies were performed on 82 patients with lymphadenopathy, immunosuppression, and possible acquired immune deficiency syndrome. the 21 patients with diagnoses of lymphoma or kaposi's sarcoma were older, had more sexual contacts, and were sexually active for more years than patients with benign diagnoses. cytomegalovirus and epstein-barr viral titers were elevated in both groups but were not significantly different in the benign and malignant groups. skin flora were cultu ... | 1984 | 6316870 |
demyelinating disease: association with epstein-barr virus. | 1984 | 6316879 | |
antineutrophil antibodies in infectious mononucleosis. | antineutrophil antibodies were detected in the serum of 12 of 14 patients with acute epstein-barr virus-induced infectious mononucleosis. these antibodies were present in two patients with mononucleosis and severe neutropenia, and in 10 patients with mononucleosis uncomplicated by severe neutropenia. antineutrophil antibodies were not detected in serum from five patients with cytomegalovirus-induced mononucleosis, or five renal allograft recipients with primary or reactivation epstein-barr virus ... | 1984 | 6318560 |
heterosexual and homosexual patients with the acquired immunodeficiency syndrome. a comparison of surveillance, interview, and laboratory data. | homosexual and heterosexual patients with the acquired immunodeficiency syndrome were compared by risk group. race; diagnoses; history of sexually transmitted diseases, sexual behavior, and drug use; and socioeconomic indicators differed considerably among risk groups, suggesting different risk factors for acquisition of the syndrome. patients in the homosexual, intravenous drug user, and haitian risk groups differed in their serologic response to cytomegalovirus and syphilis testing, presumably ... | 1984 | 6318632 |
human anti-pneumococci antibody produced by an epstein barr virus (ebv)-immortalized cell line. | anti-pneumococcal antibody-producing lymphocytes from a patient who was immunized with pneumovax (a polysaccharide mixture from 14 different pneumococci types) were selected and immortalized with epstein barr virus (ebv). the cells of the emerging cloned line were shown on a single cell level assay to secrete an igm, kappa anti-pneumococci antibody. this human monoclonal antibody bound specifically to polysaccharides of pneumococci type 8, but did not bind to any of the other 13 pneumococci type ... | 1984 | 6317751 |
early acquisition of cytomegalovirus antibodies in communal settlements (kibbutzim) in israel. | the kibbutzim (communal settlements) in israel are unique societies. although socioeconomic status is above average and hygienic conditions are usually excellent, infants in these communities living together in nurseries acquire infection with cytomegalovirus and epstein-barr virus at an early age. we have studied the occurrence of specific immunoglobulin g (igg) and immunoglobulin m (igm) antibodies to cytomegalovirus and epstein-barr virus in a kibbutz in northern israel where many of the infa ... | 1984 | 6320638 |
the influence of human plasma lipoproteins and fatty acids in immunological reactions. | 1984 | 6320687 | |
inhibition of polyclonally induced immunoglobulin secretion by aurothiomalate. | the influence of sodium aurothiomalate on the secretion of immunoglobulins by normal human lymphocytes in vitro was investigated by means of a reverse hemolytic plaque forming cell (pfc) assay. aurothiomalate inhibited the pfc response induced by pokeweed mitogen (pwm) and by epstein-barr virus (ebv) in a dose dependent manner. the inhibition was irreversible, as pre-incubation for 2 h with the drug followed by extensive washing and further culture in gold salt-free medium still caused an inhibi ... | 1984 | 6320688 |
the effect of tinidazole on primary ebv infection and immunocompetence. | tinidazole and metronidazole have been reported beneficial in the treatment of acute anginose infectious mononucleosis. as this disease is caused by the epstein-barr virus (ebv), the effect of tinidazole on ebv infection of human b-lymphocytes was investigated. tinidazole had no effect on induction of ebv determined nuclear antigen (ebna) or dna synthesis, while immunoglobulin synthesis was increased in the presence of the drug. cytotoxicity directed against ebv positive cell lines and long term ... | 1984 | 6320359 |
early events in the infection of human b lymphocytes by epstein-barr virus: the internalization process. | the early events in the infection of normal b lymphocytes and b lymphoblastoid cells by epstein-barr virus (ebv) were examined by electron and immunoelectron microscopy and by infectivity and inhibition studies. purified ebv remained on the cell surface at 4 degrees and appeared as 250-nm ovoid particles in contact with the cell membrane through 50-nm envelope projections. internalization of ebv in normal b lymphocytes into large (300-500 nm) uncoated vacuoles was initiated within 2 to 5 min at ... | 1984 | 6320532 |
the capsid antigen of epstein-barr virus in nasopharyngeal carcinomas: comparison of serum antibody titre, cellular antigen content and histopathology. | in a retrospective study of 21 patients with nasopharyngeal malignancies (lymphoepithelial carcinomas, both undifferentiated and dedifferentiated serological and immunohistological methods were used to examine the significance of the igg- and igm-antibody against the capsid antigen of epstein-barr virus for early detection and follow-up, and the findings compared with those from a control group. furthermore, the iga-antibody is taken into account and presented with examples from the literature. ... | 1984 | 6318710 |
establishment of epstein-barr virus (ebv)-associated nuclear antigen (ebna)-positive nasopharyngeal carcinoma hybrid cell line (npc-kt). | primary nasopharyngeal carcinoma (npc) cells were fused to hypoxanthine-guanine phosphoribosyltransferase (hgprt)-defective cells derived from adenoid tissues using sendai virus. some of the fused cells developed into epithelial-like hybrid cells in a selective hat medium. the hybrid cells (npc-kt) were epstein-barr virus (ebv)-associated nuclear antigen (ebna)-positive cells. there have been no reports on the establishment of ebna-positive epithelial cell lines derived from npc. thus, the epith ... | 1984 | 6318711 |
evidence for integrated ebv genomes in raji cellular dna. | human lymphoid cell lines cannot be grown in long-term tissue culture, as a rule, unless the cells have been transformed by epstein-barr virus (ebv). the latent ebv dna in established cell lines, is mainly present as free covalently closed circles but viral dna sequences with properties of integrated dna also seem to be present. we have extended the studies on the physical state of the eb viral dna sequences in the cell line raji which appear at a lower density than that for free eb viral dna du ... | 1984 | 6320116 |
possible dna-rna tumor virus interaction in human lymphomas: expression of retroviral proteins in ramos lymphoma lines is enhanced after conversion with epstein-barr virus. | epstein-barr virus (ebv)-genome-negative human lymphoma lines, ramos and bjab, can be converted by ebv in vitro into ebv-genome-positive virus nonproducer lines. these cell lines have been used to identify surface antigens unique to ebv, with the expectation that such determinants might be related to the antigenic target responsible for ebv-specific immunosurveillance. antisera prepared in rabbits immunized with either whole cells or purified plasma membranes were used in immunoblot experiments ... | 1984 | 6320170 |