Publications

ecotoxicological characterization of industrial wastewater--sulfite pulp mill with bleaching.	different process wastewaters from a sulfite pulp mill with bleaching were characterized by chemical analysis and toxicity tests. the amount of adsorbable organically bound halogen (aox) from the bleachery was 3.6 kg per ton pulp. the extractable organically bound chlorine was 15% of aox. some identified organochlorine compounds in the effluent could be traced in the receiving water. effluents from the chlorination and alkaline extraction stages and the condensate were the main contributors to t ...	1989	2612424
effects of postnatal dexamethasone treatment on development of alveoli in adult rats.	we compared the effects of dexamethasone given to rats from age 4 to 14 days, on alveolar development at age 99 days. judged by similar nose-to-tail length and body weight, somatic growth was not altered by dexamethasone treatment. lung volumes in both air- and saline-filled lungs and mean chord length (lm) were increased, whereas alveolar surface area (sa) and surface-to-volume ratio (s/v) were diminished in dexamethasone-treated adult rats. these changes were associated with a significant redu ...	1989	2612450
effects of postnatal dexamethasone treatment on development of alveoli in adult rats.	we compared the effects of dexamethasone given to rats from age 4 to 14 days, on alveolar development at age 99 days. judged by similar nose-to-tail length and body weight, somatic growth was not altered by dexamethasone treatment. lung volumes in both air- and saline-filled lungs and mean chord length (lm) were increased, whereas alveolar surface area (sa) and surface-to-volume ratio (s/v) were diminished in dexamethasone-treated adult rats. these changes were associated with a significant redu ...	1989	2612450
pathophysiological variations in the rat liver plasma membrane serine proteinase activity.	the effects of fasting, diabetes, cholestasis, two-third hepatectomy and adrenalectomy on the rat liver plasma membrane serine proteinase activity were studied. our results show a significant decrease of the enzyme activity during fasting (-50%), during experimental diabetes (-50%), in regenerating liver after partial hepatectomy (-70%) and after extrahepatic cholestasis (-70%). no modifications are noted when the rats are bilaterally adrenalectomized. these findings suggest that the enzyme acti ...	1989	2612451
pathophysiological variations in the rat liver plasma membrane serine proteinase activity.	the effects of fasting, diabetes, cholestasis, two-third hepatectomy and adrenalectomy on the rat liver plasma membrane serine proteinase activity were studied. our results show a significant decrease of the enzyme activity during fasting (-50%), during experimental diabetes (-50%), in regenerating liver after partial hepatectomy (-70%) and after extrahepatic cholestasis (-70%). no modifications are noted when the rats are bilaterally adrenalectomized. these findings suggest that the enzyme acti ...	1989	2612451
characterization of excretory-secretory proteins synthesized in vitro by schistosoma mansoni primary sporocysts.	excretory-secretory (e-s) products released by larval schistosomes have been implicated in the interference of host snail defense systems. because of the potentially important role that e-s products play in the parasite-host relationship, total and newly synthesized e-s proteins from in vitro-cultured schistosoma mansoni primary sporocysts were characterized using incorporation of [35s]methionine followed by sodium dodecylsulfate polyacrylamide gel electrophoresis (sds-page) and fluorography. to ...	1989	2614597
indomethacin blocks the anorexic action of interleukin-1.	it has been reported recently that the central nervous system actions of interleukin-1 are mediated by the prostaglandin system in the brain. the present study was therefore performed in order to examine the hypothesis that indomethacin, an inhibitor of prostaglandin biosynthesis, might alleviate the interleukin-1-induced suppression of food intake in rats. the i.p. injection of interleukin-1 (2 micrograms/rat) resulted in a significant decrease in food intake. the pre-injection of indomethacin ...	1989	2620696
indomethacin blocks the anorexic action of interleukin-1.	it has been reported recently that the central nervous system actions of interleukin-1 are mediated by the prostaglandin system in the brain. the present study was therefore performed in order to examine the hypothesis that indomethacin, an inhibitor of prostaglandin biosynthesis, might alleviate the interleukin-1-induced suppression of food intake in rats. the i.p. injection of interleukin-1 (2 micrograms/rat) resulted in a significant decrease in food intake. the pre-injection of indomethacin ...	1989	2620696
synaptonemal complex analysis of hybrid cattle. i. pachytene substaging and the normal full bloods.	meiotic chromosome pairing abnormalities in full-blood and hybrid bos taurus and b. indicus cattle have been surveyed by electron microscopy of pachytene synaptonemal complex spreads. in this paper the full-blood spreads are described in detail, including the use of xy and nucleolar morphology and other measured parameters for pachytene substaging. pairing abnormalities were observed in up to 9% of the full-blood spreads. most of these pairing abnormalities (83%) occurred in early-pachytene spre ...	1989	2620818
synaptonemal complex analysis of hybrid cattle. i. pachytene substaging and the normal full bloods.	meiotic chromosome pairing abnormalities in full-blood and hybrid bos taurus and b. indicus cattle have been surveyed by electron microscopy of pachytene synaptonemal complex spreads. in this paper the full-blood spreads are described in detail, including the use of xy and nucleolar morphology and other measured parameters for pachytene substaging. pairing abnormalities were observed in up to 9% of the full-blood spreads. most of these pairing abnormalities (83%) occurred in early-pachytene spre ...	1989	2620818
[experimental and clinical studies on the exclusion and bypassing operation for abdominal aortic and iliac artery aneurysms].	for the purpose of simplifying operative techniques and decreasing operative risks, we evaluated excusion and bypassing operation for infrarenal abdominal aortic and iliac artery aneurysm (aaa) both experimentally and clinically. this method includes dissection and closure of proximal stump of the aneurysm and anatomical bypass technique in principle. in the experiments, the ratio of internal pressure of the excluded aorta (e.ao) to systemic blood pressure (e.p.i.) was 0.62 +/- 0.03 (n = 7, mean ...	1989	2626156
[experimental and clinical studies on the exclusion and bypassing operation for abdominal aortic and iliac artery aneurysms].	for the purpose of simplifying operative techniques and decreasing operative risks, we evaluated excusion and bypassing operation for infrarenal abdominal aortic and iliac artery aneurysm (aaa) both experimentally and clinically. this method includes dissection and closure of proximal stump of the aneurysm and anatomical bypass technique in principle. in the experiments, the ratio of internal pressure of the excluded aorta (e.ao) to systemic blood pressure (e.p.i.) was 0.62 +/- 0.03 (n = 7, mean ...	1989	2626156
increased erythrocyte adenosine deaminase activity in children with perinatal human immunodeficiency virus infection.	erythrocyte adenosine deaminase (ada) activity was assessed in 33 children born to human immuno-deficiency virus (hiv)-positive mothers. the enzyme values were significantly increased in infected, symptom-free children compared with a control group of hiv-negative subjects (mean +/- sd: 0.34 +/- 0.01 unit/ml red blood cells (rbc) vs. 0.25 +/- 0.04 unit/ml rbc, p less than 0.01) and a further significant increase was found in symptomatic children (0.45 +/- 0.02 unit/ml rbc, p less than 0.01 vs. i ...	1989	2626286
[effects of smoking on human health].	in this study, 30 cases of male smokers aged 35.7 +/- 5.2 years mean and 30 male nonsmokers aged 36.6 +/- 6.8 years were selected from a factory. before the assay, everyone smoked one pack of cigarette in a day, after that these tests were done. the experimental results as follows. 1. comparing with control group, the levels of igg and igm in serum of smoking group are lower. it was shown that smoking has an inhibitive effect on phagocytosis and bactericidal of am, so smoking has a damaging and ...	1989	2632033
[effects of smoking on human health].	in this study, 30 cases of male smokers aged 35.7 +/- 5.2 years mean and 30 male nonsmokers aged 36.6 +/- 6.8 years were selected from a factory. before the assay, everyone smoked one pack of cigarette in a day, after that these tests were done. the experimental results as follows. 1. comparing with control group, the levels of igg and igm in serum of smoking group are lower. it was shown that smoking has an inhibitive effect on phagocytosis and bactericidal of am, so smoking has a damaging and ...	1989	2632033
superoxide dismutase activity in some strains of lactobacilli: induction by manganese.	dialyzed cell-free extract of lactobacilli was found to contain superoxide dismutase activity by using a test system in which superoxide ion is generated by xanthine oxidase. the specific activities of lactobacillus acidophilus atcc 4356, lactobacillus murinus atcc 35020, lactobacillus acidophilus crl 358, lactobacillus plantarum atcc 8014, lactobacillus casei crl 431, lactobacillus plantarum crl 353, lactobacillus fermentum atcc 9338, lactobacillus buchneri ncdo 110, and lactobacillus fermentum ...	1989	2632048
superoxide dismutase activity in some strains of lactobacilli: induction by manganese.	dialyzed cell-free extract of lactobacilli was found to contain superoxide dismutase activity by using a test system in which superoxide ion is generated by xanthine oxidase. the specific activities of lactobacillus acidophilus atcc 4356, lactobacillus murinus atcc 35020, lactobacillus acidophilus crl 358, lactobacillus plantarum atcc 8014, lactobacillus casei crl 431, lactobacillus plantarum crl 353, lactobacillus fermentum atcc 9338, lactobacillus buchneri ncdo 110, and lactobacillus fermentum ...	1989	2632048
ozagrel hydrochloride monohydrate, a thromboxane synthase inhibitor, and its metabolites as inhibitors of hepatic microsomal drug metabolism.	the change in the hepatic oxidative drug-metabolizing capacity in humans treated with ozagrel hydrochloride monohydrate (oza), an imidazole derivative and a new thromboxane a2 synthase inhibitor, was studied and the inhibitory potencies of the metabolites of oza (m-1 and m-2) on the mouse hepatic microsomal monooxygenase system in vitro were compared with that of oza. in vitro, m-1 and m-2, which are the beta-oxidized form and the reduced form of oza, respectively, inhibited aminopyrine n-demeth ...	1989	2632083
ozagrel hydrochloride monohydrate, a thromboxane synthase inhibitor, and its metabolites as inhibitors of hepatic microsomal drug metabolism.	the change in the hepatic oxidative drug-metabolizing capacity in humans treated with ozagrel hydrochloride monohydrate (oza), an imidazole derivative and a new thromboxane a2 synthase inhibitor, was studied and the inhibitory potencies of the metabolites of oza (m-1 and m-2) on the mouse hepatic microsomal monooxygenase system in vitro were compared with that of oza. in vitro, m-1 and m-2, which are the beta-oxidized form and the reduced form of oza, respectively, inhibited aminopyrine n-demeth ...	1989	2632083
long residual activity of bacillus sphaericus 1593 against culex quinquefasciatus larvae in artificial pools.	the microbial larvicide, bacillus sphaericus 1593, was evaluated against culex quinquefasciatus larvae in artificial pools at huay kwang area in bangkok between march and october 1985. this study was aimed at determining the residual activity of b. sphaericus 1593 in waste water under field conditions. the larvicidal activity of b. sphaericus 1593 was found to persist for at least 5 months in artificial pools containing waste water. the populations of b. sphaericus 1593 in the test water fluctua ...	1989	2633350
distribution, oxygenation, and clonogenicity of macrophages in a murine tumor.	transplantable murine tumors, such as the squamous cell carcinoma growing in c3h mice, include a significant proportion of normal cells. the nature of these cells, their locations relative to the blood supply, their oxygenation status, and ability to incorporate 3h-thymidine were examined by sorting cells staining positive or negative with fluorescein isothiocyanate-conjugated goat antimouse igg. of the cells that were recovered from this tumor, 39% +/- 19 (n = 25) were igg+ cells, and this perc ...	1989	2639726
molecular studies of human response to allergens.		1989	2639765
recurrent shigellosis complicating human immunodeficiency virus infection: failure of pre-existing antibodies to confer protection.		1989	2642653
clinical applications of recombinant human colony-stimulating factors.	the differentiation and maturation of hematopoietic progenitor cells are regulated by certain growth factors. several of these glycoproteins have been characterized, and their amino acid sequences have been delineated. modern dna technology provides sufficient quantities of these hormones for testing in clinical trials. erythropoietin (epo) has been shown to increase the hemoglobin level and hematocrit in patients with end-stage renal disease. granulocyte colony-stimulating factor (g-csf) and gr ...	1989	2642725
intracellular spread of shigella flexneri associated with the kcpa locus and a 140-kilodalton protein.	escherichia coli k-12 hybrids carrying both the 220-kilobase plasmid and the pure-linked kcpa locus from shigella flexneri expressed a 140-kilodalton (kda) protein which was recognized by convalescent sera from monkeys infected with s. flexneri. these hybrids were tested for the ability to produce plaques in hela cell monolayers. hybrid strains which carried both the 220-kilobase plasmid and the kcpa locus had a plaque-forming efficiency of at least 10(-4) pfu/cfu, whereas the plaque-forming eff ...	1989	2643571
characterization of opiate binding sites on membranes of rat lymphocytes.	in view of the importance of membrane receptors for the interconnection between the central nervous system and the immune system, we carried out a study to characterize opiate binding sites on membranes of rat lymphocytes. we found that mitogen-activated spleen cells, but not thymocytes, possess specific and displaceable binding sites for [3h]naloxone. the binding was equally effective and intense while using b-cell-depleted spleen cells. the binding showed two sites of saturation, one at 10 nm ...	1989	2643626
characterization of opiate binding sites on membranes of rat lymphocytes.	in view of the importance of membrane receptors for the interconnection between the central nervous system and the immune system, we carried out a study to characterize opiate binding sites on membranes of rat lymphocytes. we found that mitogen-activated spleen cells, but not thymocytes, possess specific and displaceable binding sites for [3h]naloxone. the binding was equally effective and intense while using b-cell-depleted spleen cells. the binding showed two sites of saturation, one at 10 nm ...	1989	2643626
invasion of vero cells by salmonella species.	the invasiveness of salmonella strains for vero cells was studied by quantitative bacteriology; the technique was more sensitive than phase contrast microscopy. all of 59 salmonella strains, of 19 different serotypes, were more invasive than escherichia coli k12. three strains of shigella were as invasive as most of the salmonella strains whereas 29 strains of e. coli, two of proteus, three of klebsiella and one of serratia were much less invasive. two citrobacter strains exhibited intermediate ...	1989	2643706
virg, a plasmid-coded virulence gene of shigella flexneri: identification of the virg protein and determination of the complete coding sequence.	on the 230-kilobase-pair (kb) virulence plasmid of shigella flexneri 2a strain ysh6000, at least seven separate genetic determinants have been identified. one of them, an approximately 4-kb region, virg, that is required for the sereny reaction, was extensively studied to examine the role of the virg region. the phenotype of a virg- mutant (m94) of ysh6000 in the cytoplasm of cultured mk cells was characterized by a kinetic study of the invading shigellae. the observed phenotype of m94 in the cy ...	1989	2644195
chronic shigella flexneri infection preceding development of acquired immunodeficiency syndrome.	shigella sp. is known to be an important cause of diarrhea in homosexual men, although chronic infection is infrequently recognized. we describe recurrent and relapsing symptomatic infection due to shigella flexneri in a human immunodeficiency virus-infected homosexual man subsequently developed acquired immunodeficiency syndrome. patients with acquired immunodeficiency syndrome may be prone to developing chronic shigellosis because of impaired intestinal cell-mediated immunity.	1989	2644299
shigellosis and aids. report of a case and brief review of the literature.	this report describes the fatal outcome of an infection with shigella flexneri in a 39-yr-old man with the acquired immunodeficiency syndrome (aids). the pertinent literature is reviewed.	1989	2644558
ketopantoyl-lactone reductase from candida parapsilosis: purification and characterization as a conjugated polyketone reductase.	ketopantoyl-lactone reductase (2-dehydropantoyl-lactone reductase, ec 1.1.1.168) was purified and crystallized from cells of candida parapsilosis ifo 0708. the enzyme was found to be homogeneous on ultracentrifugation, high-performance gel-permeation liquid chromatography and sds-polyacrylamide gel electrophoresis. the relative molecular mass of the native and sds-treated enzyme is approximately 40,000. the isoelectric point of the enzyme is 6.3. the enzyme was found to catalyze specifically the ...	1989	2644973
role of m cells in initial antigen uptake and in ulcer formation in the rabbit intestinal loop model of shigellosis.	strains of shigella flexneri with different invasive and pathogenic potentials were inoculated into the intestinal lumen of acutely ligated loops in nonimmune rabbits. after 90 min, tissues processed for ultrastructural as well as light microscopy showed that the bacilli were phagocytosed by m cells over lymphoid follicles of peyer's patches and carried in vacuoles into the epithelium. nonpathogenic as well as pathogenic strains were readily taken up regardless of the presence of the 140-megadal ...	1989	2645214
association of ki-ras with amplified dna sequences, detected in human ovarian carcinomas by a modified in-gel renaturation assay.	a modified in-gel dna renaturation technique, which detects dna sequences amplified greater than 7-fold in human dna, was used to analyze gene amplification in surgical specimens of primary and metastatic ovarian carcinomas. amplified dna sequences were detected in two of eight tumors. hybridization of these samples with different oncogene probes revealed that both tumors contained an amplified ki-ras gene, which in one case was coamplified with c-myc. in one of the tumors, ki-ras was found to b ...	1989	2647292
association of ki-ras with amplified dna sequences, detected in human ovarian carcinomas by a modified in-gel renaturation assay.	a modified in-gel dna renaturation technique, which detects dna sequences amplified greater than 7-fold in human dna, was used to analyze gene amplification in surgical specimens of primary and metastatic ovarian carcinomas. amplified dna sequences were detected in two of eight tumors. hybridization of these samples with different oncogene probes revealed that both tumors contained an amplified ki-ras gene, which in one case was coamplified with c-myc. in one of the tumors, ki-ras was found to b ...	1989	2647292
identification of cell types containing s-100b protein-like immunoreactivity in the islets of langerhans of the guinea pig pancreas with light and electron microscopy.	cell types containing s-100b protein-like immunoreactivity in the islets of langerhans of the guinea pig were studied by light- and electron-microscopic immunocytochemistry using antisera to s-100b protein, insulin, glicentin, somatostatin, and pancreatic polypeptide. two types of s-100b-immunoreactive cells were identified. the first type was stellate and characterized by thin cytoplasmic processes sheathing endocrine-type cells, especially pancreatic a-cells. it was located predominantly in th ...	1989	2647297
identification of cell types containing s-100b protein-like immunoreactivity in the islets of langerhans of the guinea pig pancreas with light and electron microscopy.	cell types containing s-100b protein-like immunoreactivity in the islets of langerhans of the guinea pig were studied by light- and electron-microscopic immunocytochemistry using antisera to s-100b protein, insulin, glicentin, somatostatin, and pancreatic polypeptide. two types of s-100b-immunoreactive cells were identified. the first type was stellate and characterized by thin cytoplasmic processes sheathing endocrine-type cells, especially pancreatic a-cells. it was located predominantly in th ...	1989	2647297
heterotopic heart transplantation: a reliable option for a select group of high-risk patients.	from july 1982 through february 1988, 229 patients underwent heart transplantations at the texas heart institute, houston, 11 of whom had heterotopic transplantations. indications for the heterotopic procedure included pulmonary hypertension (eight patients) and marked body-weight mismatch (greater than or equal to 20%) between the recipient and donor (three patients). the 1-year actuarial survival rate for the heterotopic group is 80.4% compared with 77.9% for the orthotopic group. despite simi ...	1989	2647930
delivered volume during high-frequency ventilation.		1989	2648741
emergence of highly trimethoprim-sulfamethoxazole-resistant shigella in a native american population: an epidemiologic study.	resistance to trimethoprim-sulfamethoxazole (tmp-smx) emerged among shigella isolates from the navajo reservation in the southwestern united states in 1985, years after this antimicrobial agent came into common use. in the study area, tmp-smx resistance increased dramatically from 3 per cent in 1983 to 21 per cent in 1985. resistance was polyclonal and occurred in both s. sonnei and s. flexneri. no single plasmid was common to all resistant strains. however, all 28 tmp-smx resistant isolates exa ...	1989	2650535
identification of an escherichia coli gene homologous to virr, a regulator of shigella virulence.	virulence in shigella spp., as well as in strains of enteroinvasive escherichia coli, is regulated by growth temperature. previously, virr had been identified as the gene controlling the temperature-regulated expression of shigella virulence. since shigella spp. and e. coli are also known to share greater than 90% dna sequence homology, we sought to determine if nonpathogenic e. coli k-12 c600 contains a gene homologous to the shigella flexneri 2a gene virr. through the use of transduction and m ...	1989	2651420
the human gastric bactericidal barrier: mechanisms of action, relative antibacterial activity, and dietary influences.		1989	2651535
[resistance to infection. physiology and pathology of the immune system].	the contribution of observations in congenital deficiency disorders to our understanding of normal mechanisms can hardly be overestimated, as may in particular be seen in the complex field of mechanisms aiming at maintaining the individual integrity. after a brief summary of the natural lines of defense we demonstrate a few cases with typical deficiencies, i.e. of the integuments (ciliary dyskinesia syndrome), of the phagocytes (chronic granulomatous disease = cgd), of specific immune reactions ...	1989	2652037
tropical infections after renal transplantation.		1989	2652678
androgen-regulated gene expression.	while a great deal of knowledge on the mechanisms of steroid hormone regulated gene expression now exists, specific information relating to androgens is lacking. a number of experimental systems have been developed and show promise as models for molecular studies of androgen regulation. further development of these models must address the issue of whether or not the androgen receptor behaves similarly to other steroid receptors. this will require progress in the purification of the receptor itse ...	1989	2653194
t-lymphocyte clones responsive to shigella flexneri.	t lymphocytes from a patient with shigella flexneri dysentery and postdysenteric reactive arthritis were cloned by limiting dilution with recombinant interleukin-2 and a strain of s. flexneri different from that which had infected her. five of eight clones produced proliferated in response to the shigellae used to generate the clones. the response required irradiated syngeneic blood mononuclear cells as antigen-presenting cells. one such clone, mc12, proliferated in response to both the shigella ...	1989	2654177
studies with antibodies to cultured rat glomerular epithelial cells. subepithelial immune deposit formation after in vivo injection.	to investigate the role of glomerular epithelial cell (gec) membrane proteins in the in situ formation of subepithelial immune deposits, the authors raised a rabbit antiserum against gec that had been grown in culture (anti-gec). by indirect immunofluorescence (if) on normal rat kidney, anti-gec stained proximal tubular brush border (bb). after intravenous injection into animals, granular glomerular capillary wall staining for igg was present by ie and subepithelial immune deposits were identifi ...	1989	2655461
control of diarrheal diseases.	the tremendous advances made in the control of diarrheal diseases in the past few years indicate what more can be achieved. even though the lives of an estimated three quarters of a million children are being saved each year, over three million children are still dying from readily preventable diarrheal disease. the challenge is to build on the successes thus far, to learn from experience, to promote changes in health habits that will prevent diarrhea, and to make all of these activities sustain ...	1989	2655632
inoculum size in shigellosis and implications for expected mode of transmission.		1989	2656880
[determination of shigella antigens in the feces of people, immunized with enteral vaccine prepared from s. flexneri antigen].	the dynamic determination of the presence of the specific antigen and its activity in the excreta of humans subjected to enteral immunization with vaccine prepared from s. flexneri antigen was made in the agglutination test and neutralization test with the use of, respectively, antibody and antigenic erythrocyte diagnosticums. in the feces and urine of the vaccinees antibodies occurred less commonly and, as a rule, they were less active than those detected in dysentery patients at the correspond ...	1989	2658436
oral shigella vaccines.		1989	2659269
the enteric immune response to shigella antigens.	mucosal immunity to some enteropathogens occurs naturally following infection. by learning how to optimize initiation of the mucosal immune response it will be possible to develop vaccines against a wide variety of enteropathogens and their toxic products. in the past few years, we have examined stimulation of the mucosal response to shigella antigens. we have found that the mucosal memory response to shigella lps can be stimulated by oral immunization with live, but not with killed shigella. th ...	1989	2659270
electropherotyping of plasmid dna of different serotypes of shigella flexneri isolated in bangladesh.	one hundred and twenty-five shigella flexneri strains, isolated during january-december 1984, at the dhaka treatment centre of the international centre for diarrhoeal disease research, bangladesh, were serotyped using absorbed rabbit antisera specific for all type- and group-factor antigens, as well as a group of ten mouse and rat monoclonal antibodies. electropherotypes of the plasmid deoxyribonucleic acid (dna) were also determined. s. flexneri 2a was the predominant serotype followed by 3b, 1 ...	1989	2661251
renal allograft rejection: possible involvement of lymphokine-activated killer cells.	human renal allograft tissue was recovered at transplant nephrectomy from three patients with irreversible loss of graft function. this tissue was disaggregated and separated into two fractions on the basis of particle size. fraction 1 contained glomeruli and developed a mixed outgrowth containing adherent epithelial and mesangial cells after a limited period of culture. fraction 2 contained fragments of renal tubules and produced monolayers of tubular epithelial cells during culture. a populati ...	1989	2661417
inhibition of proteoglycan synthesis alters extracellular matrix deposition, proliferation, and cytoskeletal organization of rat aortic smooth muscle cells in culture.	arterial proteoglycans have been implicated in several important physiological processes ranging from lipid metabolism to regulation of smooth muscle cell growth. vascular smooth muscle (vsm) cells are the major producers of proteoglycans in the medial layer of blood vessels. to study functional consequences of alterations in vsm proteoglycan metabolism we used 4-methylumbelliferyl-beta-d-xyloside to inhibit proteoglycan synthesis in primary and early passage cultures of rat aortic smooth muscle ...	1989	2661564
[biological properties of bacteria of the species shigella flexneri].	some biological properties of s. flexneri have been studied. the strains of this bacterial species have been shown to produce dnaase in 98.8 +/- 0.77% of cases and rnaase in 97.4 +/- 1.5% of cases. the capacity for the positive reaction with congo red as early as after 24-hour incubation in the thermostat has proved to be characteristic of s. flexneri (91.1 +/- 3.6%). if stored at 4 degrees c in semiliquid agar, s. flexneri cultures have been found to retain their capacity for producing the abov ...	1989	2662686
lineage-specific gene rearrangement/deletion: a nonconservative model.	for the lymphocytic descendants of the hematopoietic differentiation pathway, characteristic gene rearrangements result in deletions of significant portions of chromosome regions specifying lymphocyte-specific gene products on either or both chromosomes of each involved pair. molecular mechanisms facilitating the rearrangement/deletion events have been elegantly and fruitfully elaborated in the years since the first documentation of their occurrence by hozumi and tonegawa (n. hozumi and s. toneg ...	1989	2663139
elimination of malignant clonogenic breast cancer cells from human bone marrow.	autologous bone marrow transplantation is a promising approach to the treatment of breast cancer but is at present limited to patients without bone marrow metastases. to eliminate malignant clonogenic breast cancer cells from normal human bone marrow, immunomagnetic separation has been combined with chemoseparation using 4-hydroperoxycyclophosphamide. breast cancer cell lines have been mixed with a 10-fold excess of irradiated human bone marrow from normal donors. mixtures have been incubated wi ...	1989	2663144
chloroplast and nuclear genomes of chlamydomonas reinhardtii share homology with escherichia coli genes for dna replication, repair and transcription.	most of the cpdna genes studied to date are genes encoding elements of the photosynthetic apparatus and translational machinery. much less is known about genes encoding the polypeptides involved in transcription, cpdna replication, recombination and repair. the similarities between bacterial and some cpdna genes were exploited to identify some of these chloroplast genes using bacterial probes. probes derived from the escherichia coli genes dnaa, reca, uvrc, transcriptional factor rho, and rpoc w ...	1989	2663186
congo red-mediated regulation of levels of shigella flexneri 2a membrane proteins.	the ability of shigella spp. to bind congo red from agar medium is generally correlated with their virulence properties. we used a metabolically active culture of shigella flexneri 2a to determine the effect of congo red on its membrane protein profiles. virulent s. flexneri grown in the presence of congo red at 37 degrees c showed increased levels of three proteins with mrs of 43,000, 58,000, and 63,000 (43k, 58k, and 63k proteins) in the sarkosyl-soluble membrane fractions. the observed phenom ...	1989	2663721
plasmid-associated adherence of shigella flexneri in a hela cell model.	the initial interaction of shigella flexneri with hela cells was studied at 4 degrees c, a temperature that inhibits parasite-directed endocytosis. it was found that invasive strains were 10-fold more adherent to hela cells than were isogenic, noninvasive strains which had lost a 140-megadalton plasmid. adherent strains were also more hydrophobic than were nonadherent strains.	1989	2663729
use of monoclonal antibodies to type shigella flexneri in bangladesh.	a panel of 10 mouse and rat monoclonal antibodies specific for different type- and group-specific o-antigenic determinants of shigella flexneri lipopolysaccharide was used to serotype 240 isolates of s. flexneri from bangladesh. three immunoglobulin m antibodies were used in a direct slide agglutination test; seven immunoglobulin g antibodies were absorbed to staphylococcus aureus and used in a coagglutination assay. all but 13 of the isolates could be serotyped by using the monclonal antibodies ...	1989	2666435
percutaneous nephrostomy in pyonephrosis.	a series of 76 pyonephrotic kidneys in 73 patients were drained by percutaneous nephrostomy (pn) tube and examined to evaluate the contribution of this technique to the treatment of pyonephrosis. in 71 patients, clinical symptoms disappeared 24-48 h after the procedure. two patients died from sepsis subsequent to anuria and underlying malignancy. once the acute phase had remitted, interventional procedures were carried out in 39 cases, and constituted the definitive therapy in 36. in 32 cases, e ...	1989	2667249
mechanics of the healed meniscus in a canine model.	the mechanical behavior of the intact canine stifle joint was studied, using a surgical model of meniscal injury and repair. thirty-eight animals were divided into five study groups: group s received only a sham arthrotomy (without a meniscal incision), groups p13 and p26 received peripheral medial meniscal incisions, and groups r13 and r26 received radial medial meniscus incisions. all meniscal incisions were repaired anatomically with absorbable suture. groups p13 and r13 were sacrificed at 13 ...	1989	2667375
internalization of shigella flexneri into hela cells occurs without an increase in cytosolic ca2+ concentration.	pathogenic shigella flexneri invades epithelial cells through directed phagocytosis. the role of intracellular free calcium ([ca2+]i) as a signal for this process was tested. no variation in the [ca2+]i could be detected by using the fluorescent indicator fura 2 to measure the [ca2+]i in hela cells during the invasion process. in addition, neither clamping nor maximal decreasing or increasing of the [ca2+]i of hela cells had any effect on their susceptibility to invasion. these data demonstrate ...	1989	2668195
purification, crystallization, and preliminary x-ray diffraction studies of the flavoenzyme mercuric ion reductase from bacillus sp. strain rc607.	the flavoenzyme mercuric ion reductase from bacillus sp. strain rc607 was purified by dye-ligand affinity chromatography. the protein was crystallized from solutions of high ionic strength, and one of the two crystal forms obtained has proven suitable for x-ray diffraction studies. preliminary analysis showed that these crystals belong to the tetragonal space group 1422. the unit cell dimensions are a = b = 180.7 a; c = 127.9 a. the diffraction pattern extends to better than 3 a resolution. crys ...	1989	2668287
molecular cloning and characterization of chromosomal virulence region kcpa of shigella flexneri.	in shigella flexneri, in addition to several well-recognized plasmid-borne virulence loci, at least three genetic loci implicated in pathogenesis have been recognized on the chromosome. to understand more about the pathogenesis of bacillary dysentery at a molecular level, the genetically recognized but previously unidentified kcpa region (one of the chromosomal regions near pure) was cloned and sequenced. a single translatable open reading frame encoding a 12310 dalton protein corresponding to t ...	1989	2668687
fungistatic activity of miconazole against candida albicans in relation to ph and concentration of nonprotonated drug.	at less than 10(-5) m, miconazole (mcz) exerts a fungistatic effect on candida albicans, presumably by interfering with ergosterol biosynthesis. the imidazole moiety of mcz is subject to protonation (pka approximately 6.5). based on pka and greater water solubility of protonated (mczh+) versus nonprotonated (mczo) drug, fungistatic action ought to be markedly affected by environmental ph, but apparently it is not. in this report growth phase, ph, and concentrations of mcz and mczo have been stud ...	1989	2668754
[bacillary dysentery--1987].		1989	2669032
liver disease associated with exposure to 1,1,1-trichloroethane.	1,1,1-trichloroethane is a halogenated hydrocarbon solvent commonly used in industry because of its supposed lack of hepatotoxicity. nonetheless, animal studies performed by several independent groups have shown the solvent to induce fat deposition, vacuolar degeneration, and centrilobular necrosis, changes similar to those seen after exposure to carbon tetrachloride, albeit of a much reduced magnitude, in animals exposed to the agent. four patients with fatty liver disease whose work entailed s ...	1989	2669664
oviducal storage of spermatozoa in the turkey: its relevance to artificial insemination technology.	1. this paper addresses questions concerning the role of oviducal sperm selection and storage in the relative inefficiency of turkey reproduction. 2. before the onset of egg production, the turkey has the biological potential to store spermatozoa in its oviduct for periods up to 5 weeks; after the onset of production fewer spermatozoa are retained by the oviduct. 3. a better understanding of the biological mechanisms which regulate sperm selection and storage in the turkey oviduct is necessary t ...	1989	2670069
putative start codon ttg for the regulatory protein virg of the hairy-root-inducing plasmid pria4.	the nucleotide sequence of the virg gene for a transcriptional activator on the agropine-type hairy-root-inducing plasmid pria4 was determined. the sequence contained one possible open reading frame. the gene product with a molecular size of 26.5 kda was identified by an escherichia coli coupled-transcription-translation system using cloned virg plasmids as templates. however, neither an atg nor a gtg start codon which could give rise to such a protein was identified in the nucleotide sequence. ...	1989	2670679
interleukin-1 potentiates granulopoiesis and thrombopoiesis by producing hematopoietic factors in vivo.	in vivo administration of recombinant human interleukin-1 beta (rhu il-1 beta) selectively enhanced the recovery from granulocytopenia and thrombocytopenia caused by whole body irradiation, in a dose dependent manner. since il-1 itself in vitro had no colony-stimulating activity (csa), we studied whether il-1 can produce hematopoietic factors in vivo, which in turn will promote granulopoiesis and thrombopoiesis. serum from il-1 injected mice showed marked granulocyte/macrophage csa (gm-csa), but ...	1989	2671565
synthesis of prostacyclin and thromboxane a2 by rat gastric mucosa. comparison of 1,6-dimethyl-4-oxo-1,6,7,8,9,9a-hexahydro-4h-pyrido (1,1-a)-pyrimidine-3-carboxamide and cimetidine.	the production of prostacyclin (pgi2) and thromboxane a2 (txa2) was studied in an indometacin-induced ulcer model in rats. the specific activities of prostacyclin synthetase and thromboxane synthetase as well as the tissue content of these prostaglandins were determined. the anti-ulcer effect of cimetidine and a novel agent, i.e. 1,6-dimethyl-4-oxo-1,6,7,8,9,9a-hexahydro-4h-pyrido(1,1-a)-pyrimidine-3- carboxamide (chinoin-127) was compared in this respect. the indometacin treatment shifted the b ...	1989	2673252
computer-assisted structural analysis of polysaccharides with an extended version of casper using 1h- and 13c-n.m.r. data.	the computer program casper, used in the structural analysis of polysaccharides composed of repeating units, has been extended. the extended version uses either unassigned 1h- or 13c-n.m.r. chemical shifts or the complete unassigned c,h-correlation spectrum, and can predict the structure of linear and branched oligo- and poly-saccharides. the number of possible structures, consistent with sugar and methylation analysis, can be decreased by the use of 1jc,h and 3jh,h values. the database, which c ...	1989	2673508
clinical & bacteriological profiles of shigellosis in calcutta before & after an epidemic (1984-87).	patients below 5 yr of age, hospitalised for shigellosis over a period of four years (1984-87), were studied. during the epidemic of bacillary dysentery (1984) isolation of different shigella spp. as well as shigella dysenteriae type 1 was high. decreased isolation of sh. dysenteriae type 1 and increased isolation of sh. flexneri was observed during post-epidemic years (1985-87). isolation of different shigella spp. was always above 25 per cent from patients with dysentery and greater than 7 per ...	1989	2674001
identification of shigella invasion genes by isolation of temperature-regulated inv::lacz operon fusions.	penetration and multiplication within cells of the human colonic epithelium are hallmarks of shigella spp. pathogenicity. shigella spp. virulence is regulated by growth temperature. strains phenotypically virulent when grown at 37 degrees c are phenotypically avirulent when grown at 30 degrees c. the number of genes involved in shigella spp. pathogenicity and how many virulence genes are temperature regulated are unknown. to facilitate the study of temperature-regulated virulence in shigella spp ...	1989	2674014
studies on interactions of endotoxin with factors of the contact system of plasma.		1989	2675058
lymphocyte homing.	the phenomenon of "homing" is discussed with respect to patterns of lymphocyte circulation and the molecules on the surface of both endothelium and lymphocytes that mediate this process. in addition, the data are analysed in the context of a model for lymphocyte homing.	1989	2675225
transcription by rna polymerase i stimulates mitotic recombination in saccharomyces cerevisiae.	the recombination-stimulating sequence hot1 is derived from the ribosomal dna array of saccharomyces cerevisiae and corresponds to sequences that promote transcription by rna polymerase i. when inserted at a chromosomal location outside the ribosomal dna array, hot1 stimulates mitotic recombination in the adjacent sequences. to investigate the relationship between transcription and recombination, transcription promoted by hot1 was directly examined. these studies demonstrated that transcription ...	1989	2677675
a 101-kilodalton heme-binding protein associated with congo red binding and virulence of shigella flexneri and enteroinvasive escherichia coli strains.	the ability of shigella flexneri to bind congo red or hemin is associated with virulence. a 101-kilodalton (kda) protein responsible for this phenotype (crb+) in s. flexneri was identified by a tetramethylbenzidine staining procedure which detects heme-protein complexes in polyacrylamide gels. labeling of cell-surface polypeptides with 125i revealed that the 101-kda heme-binding protein is expressed on the cell surface. expression of the protein was regulated by growth temperature and was found ...	1989	2680975
orientation of reaction center complexes from rhodobacter sphaeroides in proteoliposomes and the effect of o-phenanthroline on electrogenesis during primary photochemical reaction.	the orientation of rhodobacter sphaeroides reaction center complexes (rc complexes) in proteoliposomal membranes was investigated by a direct electrometric method. conditions were found that allow monitoring of only that rc complex fraction that is oriented with its donor side to the inner part of the proteoliposome. it is shown that o-phenanthroline, an inhibitor of electron transfer between primary (qa) and secondary (qb) quinone acceptors, can also inhibit the photoinduced qa reduction. the e ...	1989	2681179
herpes simplex infection associated with pemphigus vulgaris. case report and literature review.	a 55-year-old man had an 8-week history of oral ulcers. studies revealed concurrent pemphigus vulgaris and active herpes simplex infection. there have been seven previous case reports of this association, and these are reviewed.	1989	2681299
indicators of spontaneous and stimulated nitroblue tetrazole test of polymorphous-nuclear leucocytes in acute dysentery patients.	140 healthy individuals and 93 sick with acute dysentery were subjected to an examination by spontaneous and by bacterial preparations stimulated reaction with nitroblue tetrazole (nbt test). indicators in healthy persons were normal in the spontaneous, and increased in the nbt test, stimulated by bacterial preparations. indicators of the spontaneous nbt test in patients with acute dysentery were raised with a maximum in the period of early convalescence. stimulation by a live shigella culture-- ...	1989	2681402
the presence of nicotine on root surfaces of periodontally diseased teeth in smokers.	there is a growing body of scientific evidence to support the concept that the use of tobacco products significantly contributes to the progression of periodontal disease or is detrimental to healing following periodontal therapy. several studies have shown toxic effects of nicotine on peripheral circulation and the immune response. the purpose of the present study was to identify and compare the quantity of nicotine present on root planed and nonroot planed surfaces of teeth from smokers. twent ...	1989	2681674
mechanism of action of gnrh-induced oocyte maturation.		1989	2681744
mucoepidermoid carcinoma arising in a lymphoepithelial cyst--report of a case and review of the literature on branchiogenic carcinoma.	a 59-year-old japanese man who had recently noticed a gradual enlargement in a nodule located in the left anterior part of his neck was admitted for surgical treatment. the histopathological features of the resected tumor were consistent with a typical lymphoepithelial cyst, however, the inner surface epithelium of the cyst showed histological transition to mucoepidermoid carcinoma, which was partially invading the cyst wall. despite clinical efforts in a systemic search, no primary lesions whic ...	1989	2681906
[coexistence of abdominal aortic aneurysm and intraabdominal malignancy; two case reports].	we experienced two cases of abdominal aortic aneurysm which had intra-abdominal malignancy (early gastric cancer). case 1 was a 72 year-old man who was treated by two-stage operation of them. gastrectomy was performed about 7 months prior to the aneurysmectomy. case 2 was a 70 year-old man who was diagnosed both lethal diseases, renal dysfunction, chronic respiratory failure and multiple ventricular arrhythmia. he was treated by one-stage operation and was discharged with no complications. coexi ...	1989	2682201
a comparison of structural features of the walls of coronary arteries from 10 different species.	the intimal thickness of the coronary arteries of humans less than 20 years of age was compared with that 9 other species. it was found that humans were unique amongst the species studied in showing substantial intimal thickening at an early age. the intimal thickening was associated with defects of the internal elastic lamina (iel), which in humans were not repaired, but which in other species were associated with an effective reduplication of the iel. this reformed iel appeared in other specie ...	1989	2682490
thymotaxin, a chemotactic protein, is identical to beta 2-microglobulin.	thymotaxin, an 11-kilodalton protein chemotactic for rat bone marrow hematopoietic precursors, was purified from media conditioned by a rat thymic epithelial cell line. the nh2-terminal sequence of thymotaxin was identical to that of rat beta 2-microglobulin (beta 2m). antibodies to beta 2m removed thymotaxin activity from the fraction containing the 11-kilodalton protein. chemotactic activity was observed with rat plasma beta 2m, human beta 2m, and mouse recombinant beta 2m, further supporting ...	1989	2683083
construction and evaluation of a double mutant of shigella flexneri as a candidate for oral vaccination against shigellosis.	based on studies on the genetic and molecular basis of shigella flexneri invasive properties, we have constructed and evaluated a double mutant of s. flexneri serotype 5 for utilization as a live attenuated oral vaccine against shigellosis. the first mutation, icsa, blocks intracellular spread of bacteria as well as cell-to-cell infection. it affects the capacity of the invasive pathogen to form large abscesses in epithelia. the second mutation, iuc, eliminates production of the siderophore aero ...	1989	2683460
interspecies gene transfer in vivo producing an outbreak of multiply resistant shigellosis.	in 1983, a small outbreak of infections caused by a previously unrecognized multiply-drug-resistant shigella flexneri 3a strain occurred on the hopi indian reservation. the index patient, a diabetic woman with recurrent escherichia coli bacteriuria on prophylactic trimethoprim/sulfamethoxazole (tmp/smz) therapy, was hospitalized with concurrent e. coli urinary tract infection and shigellosis. both e. coli isolated from her urine and s. flexneri isolated from her stool were resistant to ampicilli ...	1989	2685126
[problems in the epidemiology and prevention of intestinal infections].		1989	2686152
[modern theoretical and practical conceptions of the epidemiology of shigellosis].		1989	2686310
multicentre experience in the treatment of burns with autologous and allogenic cultured epithelium, fresh or preserved in a frozen state.	this report describes the clinical results obtained from a multicentre experience of the use of autologous and allogenic cultured human epidermal cells in the treatment of partial and full skin thickness burns. a laboratory has been organized to supply cultured epithelium to burns units in different cities. from may 1986 to december 1988, 58 patients with an age range of 1 to 59 years, and with burns covering between 7 and 95 per cent of the body surface area, have been treated. graftable cultur ...	1989	2686683
a novel maturation marker for human sertoli cells.	the maturation of sertoli cells at puberty is critical for the initiation and maintenance of spermatogenesis. little is known about the factors which control sertoli cell maturation. we have examined sertoli cells in testicular specimens from 44 subjects, ranging in age from 6 months to 67 years, for reactivity with a mouse monoclonal antibody (m2a) using the immunoperoxidase reaction. we found positive reactivity with prepubertal but not postpubertal sertoli cells, suggesting that the antigen d ...	1989	2687179
use of shigella flexneri ipac and ipah gene sequences for the general identification of shigella spp. and enteroinvasive escherichia coli.	the products of the ipab, ipac, and ipad genes are involved in the expression of the invasive phenotype in all species of shigella and enteroinvasive escherichia coli (eiec). dna probes derived from these genes are accurate indicators of the invasive phenotype (m. venkatesan, j. m. buysse, e. v. vandendries, and d. j. kopecko, j. clin. microbiol. 26:261-266, 1988); however, spontaneous loss of the invasion plasmid or selective deletion of invasion-associated genes may restrict the usefulness of ...	1989	2687318
isoenzyme analysis of typhoid-shigella and escherichia-shigella hybrid vaccines and their parental strains.	isoenzyme analysis can be used to measure phenotypic changes and to characterize bacterial strains. the use of isoenzyme analysis to characterize bacterial hybrid vaccine strains that were prepared for human vaccination is described in this report. the hybrid vaccine strains were two lots of salmonella typhi-shigella sonnei vaccine, two lots of escherichia coli-shigella flexneri vaccine, and their parental strains. the two lots of s. typhi-s. sonnei hybrid vaccine had only 5 of 11 reactive isoen ...	1989	2687324
complement component c1q enhances invasion of human mononuclear phagocytes and fibroblasts by trypanosoma cruzi trypomastigotes.	internalization and infectivity of trypanosoma cruzi trypomastigotes by macrophages is enhanced by prior treatment of parasites with normal human serum. heating serum or removing c1q from serum abrogates the enhancement, but augmentation of attachment and infectivity is restored by addition of purified c1q to either serum source. although both noninfective epimastigotes (epi) and vertebrate-stage tissue culture trypomastigotes (tct) bind c1q in saturable fashion at 4 degrees c, internalization b ...	1989	2687332
the induction of interferon production in fibroblasts by invasive bacteria: a comparison of salmonella and shigella species.	as the role of interferon (ifn) in host defense against facultative intracellular bacterial infections continues to expand, it has become increasingly important to understand what cell types can produce ifn following infection and/or interaction with these invasive bacteria. we have demonstrated previously that shigella flexneri was able to induce high levels of ifn in primary cultures of human and murine fibroblasts following bacterial invasion. in this study, we examined the ability of salmone ...	1989	2687612